Standard name
Human Ortholog
Description Protein required for membrane insertion of C-terminus of Cox2p; mitochondrial integral inner membrane protein; interacts genetically and physically with Mss2p and Pnt1p; similar to S. cerevisiae Oxa1, N. crassa Oxa2p, and E. coli YidC; respiratory defect of the null mutant is functionally complemented by human COX18 carrying the N-terminal 54 amino acids of S. cerevisiae Cox18p
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0 0 0 0 0
Bud 0 0 0.05 0 0 0.06 0.06 0.06 0 0.07 0.05 0 0 0 0 0.05 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0.06 0 0 0 0 0 0
Cytoplasm 0.23 0.2 0.12 0.12 0.11 0.14 0.07 0.07 0.07 0.07 0.07 0 0.6 0.63 0.69 0.28 0.33 0.36 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.07 0.06 0 0 0 0 0 0
Endosome 0.07 0.11 0.09 0.22 0.14 0 0 0 0 0 0 0 0.11 0.12 0.11 0.07 0.11 0.14 0.07 0.08 0.1 0.06 0.07 0.08
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.66 0.62 0.72 0.67 0.76 0.86 0.94 0.92 0.95 0.9 0.94 0.95 0.06 0.05 0 0.24 0.21 0.11 0.68 0.7 0.72 0.7 0.66 0.66
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0.07 0 0.14 0.14 0.08 0 0 0 0 0 0 0 0 0 0 0.05 0.06 0 0 0 0.05 0 0 0
SpindlePole 0 0 0.07 0.11 0.06 0.05 0.05 0.06 0 0.1 0 0 0.05 0.07 0.07 0.21 0.09 0.17 0 0 0 0 0 0
Vac/Vac Membrane 0.09 0.11 0 0.07 0.1 0 0 0 0 0 0 0 0.12 0.11 0.09 0.1 0.14 0.11 0 0.06 0 0.07 0.08 0.07
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 3 1 0 0 1 0 0 1 0 0 0 2 4 7 3 5 3 8 21 8 5 5 9
Bud 2 8 14 4 10 23 29 32 16 31 23 23 4 5 2 6 5 1 0 5 1 3 1 2
Bud Neck 0 0 0 0 2 3 0 0 0 1 1 1 1 0 2 1 3 2 0 5 2 6 4 8
Bud Site 0 0 1 0 1 4 4 11 7 4 8 9 0 0 1 0 0 0 – – – – – –
Cell Periphery 6 18 5 1 3 0 0 0 1 0 3 3 4 1 9 4 10 8 0 3 0 0 1 0
Cytoplasm 24 96 34 21 45 50 35 41 32 33 28 26 133 163 237 31 46 51 3 16 1 6 3 1
Endoplasmic Reticulum 0 10 8 3 0 4 1 0 0 0 4 9 7 8 9 7 9 9 0 4 4 3 1 6
Endosome 7 53 26 38 57 6 0 2 1 3 0 1 24 30 37 8 15 19 8 48 34 22 18 28
Golgi 2 1 0 0 0 0 0 0 0 0 0 0 1 1 2 4 1 4 4 10 6 8 6 8
Mitochondria 70 293 199 114 302 310 447 516 420 406 396 580 14 14 1 26 29 16 83 450 234 244 172 244
Nucleus 2 3 5 2 5 7 6 15 9 9 3 5 0 2 1 1 4 4 0 3 0 1 1 0
Nuclear Periphery 0 1 1 0 1 1 2 1 5 2 7 8 0 0 0 0 0 0 0 2 3 2 1 3
Nucleolus 2 0 1 1 0 3 2 4 3 2 2 4 0 0 0 0 0 1 0 0 0 1 0 2
Peroxisomes 7 18 39 23 30 3 5 8 3 13 1 5 5 8 14 6 8 5 0 12 16 7 10 15
SpindlePole 2 17 18 18 25 19 22 33 19 43 17 22 12 18 23 23 12 24 1 9 5 6 4 7
Vac/Vac Membrane 10 54 10 12 39 12 11 9 11 11 9 6 27 29 31 11 19 16 2 38 2 24 22 25
Unique Cell Count 106 470 275 169 398 361 474 561 443 452 421 612 221 257 344 110 138 140 122 645 328 350 262 372
Labelled Cell Count 134 575 362 237 520 446 564 672 528 558 502 702 234 283 376 131 166 163 122 645 328 350 262 372


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 7.3 4.6 4.1 4.1 3.7 3.7 3.0 3.2 3.1 3.0 3.0 2.9 5.7 5.6 5.6 5.4 5.3 5.6 6.4 6.4 6.3
Std Deviation (1e-4) 4.2 0.9 1.4 1.2 1.2 1.4 1.0 0.9 0.8 0.6 0.7 0.6 1.1 1.0 1.0 1.3 1.2 1.4 1.2 1.5 1.4
Intensity Change (Log2) – – – 0.02 -0.14 -0.16 -0.45 -0.37 -0.41 -0.42 -0.45 -0.49 0.48 0.44 0.45 0.4 0.38 0.47 0.65 0.65 0.64


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – 0 0 0
Bud Neck – – – – – – – – – – – – 0 0 0
Bud Site – – – – – – – – – – – – 0 0 0
Cell Periphery – – – – – – – – – – – – 0 0 0
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – 0 0 0
Endosome – – – – – – – – – – – – 0 0 0
Golgi – – – – – – – – – – – – 0 0 0
Mitochondria – – – – – – – – – – – – 0 0 0
Nuclear Periphery – – – – – – – – – – – – 0 0 0
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – 0 0 0
Peroxisomes – – – – – – – – – – – – 0 0 0
SpindlePole – – – – – – – – – – – – 0 0 0
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – 0 0 0
Cytoplasm – – – – – – – – – – – – 0 0 0
Nucleus – – – – – – – – – – – – 0 0 0
Vacuole – – – – – – – – – – – – 2.5 3.8 3.1

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 0.4209 0.498 0.4928 0.289 0.3394 0.3703 2.8756 3.1478 3.1349 2.6937 2.6637 3.188 0.9324 1.259 1.0661 1.2593 1.3798 1.2663
Actin 0.0184 0.007 0.0104 0.0025 0.0262 0.0098 0.0428 0.0295 0.0268 0.0048 0.0049 0.0187 0.018 0.0081 0.0067 0.0078 0.004 0.0068
Bud 0.0025 0.0039 0.0026 0.0065 0.003 0.0026 0.0032 0.0076 0.0034 0.003 0.0027 0.002 0.0013 0.0021 0.0027 0.0103 0.0012 0.0012
Bud Neck 0.005 0.0018 0.008 0.0034 0.0093 0.0054 0.002 0.0019 0.0078 0.0023 0.0014 0.0163 0.0017 0.0018 0.0018 0.0015 0.001 0.0048
Bud Periphery 0.0039 0.0108 0.0075 0.0118 0.0097 0.0056 0.0063 0.0106 0.0106 0.0135 0.0088 0.0058 0.0039 0.0038 0.0081 0.0153 0.0042 0.0035
Bud Site 0.0149 0.0384 0.0144 0.0046 0.0191 0.002 0.0087 0.0586 0.0194 0.0046 0.0046 0.0041 0.0088 0.0217 0.0251 0.0035 0.0016 0.0016
Cell Periphery 0.0016 0.0017 0.0015 0.0019 0.002 0.0006 0.0024 0.0028 0.0019 0.0066 0.0013 0.0016 0.0026 0.002 0.0025 0.0006 0.001 0.001
Cytoplasm 0.0227 0.0498 0.0259 0.0135 0.0231 0.0315 0.0391 0.0664 0.0539 0.0443 0.0275 0.0542 0.0453 0.0842 0.0399 0.054 0.0604 0.0512
Cytoplasmic Foci 0.0891 0.0963 0.1009 0.0425 0.101 0.1046 0.0979 0.1106 0.1049 0.0912 0.0455 0.0978 0.1021 0.1152 0.0774 0.0736 0.1003 0.1013
Eisosomes 0.001 0.0007 0.0011 0.0004 0.0018 0.0004 0.001 0.0011 0.0006 0.0008 0.0013 0.0009 0.0014 0.0005 0.0011 0.0009 0.0005 0.0004
Endoplasmic Reticulum 0.0064 0.0041 0.0047 0.0008 0.0024 0.0024 0.0053 0.0026 0.007 0.0014 0.0018 0.0033 0.0104 0.0083 0.01 0.0021 0.0151 0.0057
Endosome 0.1683 0.1281 0.1047 0.0738 0.135 0.1189 0.1617 0.1391 0.1376 0.0669 0.1276 0.0872 0.2013 0.1782 0.149 0.1567 0.1328 0.1592
Golgi 0.0534 0.0386 0.0432 0.0297 0.0525 0.0415 0.0812 0.0722 0.0602 0.0267 0.0818 0.068 0.0635 0.089 0.0618 0.0539 0.0661 0.09
Lipid Particles 0.0473 0.0245 0.0276 0.0228 0.0584 0.0456 0.0508 0.0149 0.0138 0.0097 0.0044 0.0248 0.0682 0.0311 0.0263 0.016 0.0066 0.0268
Mitochondria 0.3359 0.4301 0.4465 0.6043 0.3162 0.4134 0.3562 0.2792 0.4094 0.5911 0.5632 0.4206 0.3506 0.3295 0.4767 0.5107 0.4775 0.4599
None 0.1208 0.0771 0.1086 0.0859 0.1353 0.1079 0.0527 0.1342 0.0812 0.0667 0.0802 0.1141 0.056 0.0664 0.0619 0.0567 0.0925 0.0357
Nuclear Periphery 0.0125 0.0021 0.0033 0.0005 0.0039 0.0029 0.0047 0.0011 0.0022 0.0015 0.0006 0.0028 0.0026 0.002 0.0019 0.0017 0.0014 0.0015
Nucleolus 0.0034 0.0089 0.0036 0.0012 0.0038 0.0015 0.0014 0.0016 0.0014 0.0017 0.0006 0.0008 0.0022 0.0008 0.0057 0.0007 0.0007 0.0005
Nucleus 0.0051 0.0044 0.0047 0.0014 0.0029 0.0023 0.0017 0.0025 0.0018 0.0018 0.0008 0.001 0.002 0.0015 0.0036 0.001 0.0011 0.001
Peroxisomes 0.0532 0.0438 0.0625 0.0786 0.0672 0.0824 0.046 0.0396 0.0402 0.0466 0.0207 0.0637 0.0331 0.0327 0.0198 0.0217 0.0185 0.0333
Punctate Nuclear 0.009 0.0023 0.0035 0.0009 0.0049 0.0058 0.0066 0.0043 0.0018 0.0017 0.0003 0.0018 0.0016 0.0007 0.0009 0.0016 0.0011 0.0006
Vacuole 0.0188 0.0199 0.0101 0.008 0.0176 0.0081 0.0219 0.0147 0.0097 0.0088 0.0118 0.007 0.0168 0.0152 0.011 0.0058 0.0072 0.0089
Vacuole Periphery 0.0069 0.0058 0.0046 0.0049 0.0046 0.0047 0.0064 0.0049 0.0046 0.0043 0.0083 0.0035 0.0067 0.0053 0.0061 0.0037 0.0051 0.0049

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 5.8013 7.2604 11.5447 11.4142 10.5303 12.7558 8.914 11.6788 10.5299 10.4865
Translational Efficiency – – – – – – – – – –

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✔
Gene Expression ✘
Translational Efficiency –
Standard name
Human Ortholog
Description Protein required for membrane insertion of C-terminus of Cox2p; mitochondrial integral inner membrane protein; interacts genetically and physically with Mss2p and Pnt1p; similar to S. cerevisiae Oxa1, N. crassa Oxa2p, and E. coli YidC; respiratory defect of the null mutant is functionally complemented by human COX18 carrying the N-terminal 54 amino acids of S. cerevisiae Cox18p
Localization
Cell Percentages mitochondrion (94%)
Cell Cycle Regulation No
Subcompartmental Group mito-1

Cox18

Cox18


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Cox18-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available