Standard name
Human Ortholog
Description Protein proposed to be involved in vacuolar functions; mutant shows defect in CPY processing and fragmented vacuoles; deletion mutant has increased glycogen accumulation and displays elongated buds; green fluorescent protein (GFP)-fusion protein localizes to the nucleus; ENV11 has a paralog, VID22, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0.07 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.05 0.1 0.08 0.19 0.13 0.11 0.13 0.12 0.07 – 0.07 0 0.08 0.16 0.19 0.09 0.06 0.09 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0
Endosome 0.05 0 0 0 0 0 0 0 0 – 0 0 0 0.07 0.05 0 0.06 0.07 0.11 0.09 0.08 0.05 0.07 0.08
Golgi 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.43 0.43 0.08 0.22 0.21 0.48 0.47 0.31 0.67 – 0.74 0.49 0.24 0 0.12 0.08 0.05 0.14 0 0 0.05 0.06 0 0.05
Nucleus 0.52 0.53 0.65 0.52 0.6 0.53 0.57 0.6 0.56 – 0.57 0.68 0.68 0.67 0.59 0.76 0.74 0.7 0.32 0.36 0.29 0.37 0.33 0.27
Nuclear Periphery 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.07 0 0 0 0 0 0 0 0 – 0 0 0.05 0 0.06 0.06 0 0 0.19 0.12 0.15 0.13 0.14 0.15
Peroxisomes 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0.05 0.19 0.2 0.12 0.1 0.1 0.14 0 – 0.05 0.11 0 0 0 0 0 0.05 0.16 0.09 0.14 0.11 0.12 0.14
Vac/Vac Membrane 0.05 0.08 0 0 0.07 0 0 0 0 – 0 0 0 0.05 0.06 0 0.09 0.06 0.09 0.17 0.17 0.17 0.17 0.13
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 1 0 – 0 1 0 0 0 0 0 0 0 0 0 0 3 2
Bud 1 0 0 3 4 1 3 5 3 – 7 6 0 0 2 2 0 0 0 0 1 3 9 14
Bud Neck 0 0 10 4 4 2 5 16 5 – 5 7 0 0 0 0 0 0 1 2 2 3 7 12
Bud Site 0 0 0 0 0 4 4 3 11 – 11 7 1 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 6 8 12 32 25 30 43 43 23 – 19 12 18 42 60 22 11 15 0 5 6 3 4 11
Endoplasmic Reticulum 2 1 0 0 0 0 1 1 0 – 0 0 0 0 0 9 6 8 1 0 0 1 0 5
Endosome 6 1 4 7 7 9 1 3 3 – 1 2 7 20 15 8 11 11 20 11 17 7 26 34
Golgi 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 4 2 4 1 1 0 0 1 1
Mitochondria 57 36 11 37 40 125 159 115 212 – 205 136 55 12 37 19 10 22 4 4 11 9 13 23
Nucleus 69 44 93 86 115 138 193 222 178 – 158 188 155 180 189 193 140 112 60 47 67 57 115 116
Nuclear Periphery 1 1 0 0 0 1 0 0 2 – 3 0 1 0 1 3 0 0 3 3 5 1 4 4
Nucleolus 9 2 0 0 0 0 2 3 13 – 11 4 12 9 20 15 7 2 36 16 35 19 51 63
Peroxisomes 0 0 0 0 0 0 0 0 0 – 0 0 0 1 1 0 0 0 0 0 0 0 0 1
SpindlePole 2 4 27 33 23 26 34 52 14 – 15 31 9 8 7 11 7 8 29 11 31 16 44 62
Vac/Vac Membrane 6 7 2 3 14 8 4 5 9 – 3 6 6 13 20 11 17 10 16 21 38 26 60 55
Unique Cell Count 133 83 143 166 191 262 340 372 318 276 275 229 268 319 253 189 159 188 131 231 156 355 434
Labelled Cell Count 159 104 159 205 232 344 449 469 473 438 400 264 285 352 297 211 192 188 131 231 156 355 434


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.4 4.2 4.9 4.7 5.1 3.9 4.2 4.4 3.7 – 3.6 4.0 4.7 4.9 4.8 7.3 7.1 7.4 4.4 4.6 4.8
Std Deviation (1e-4) 0.8 0.8 0.9 1.6 1.5 0.9 1.2 0.9 1.2 – 1.2 0.8 1.1 1.2 1.3 1.4 1.1 1.8 1.4 1.7 1.9
Intensity Change (Log2) – – – -0.06 0.06 -0.3 -0.2 -0.14 -0.39 – -0.45 -0.28 -0.06 0.0 -0.01 0.6 0.56 0.6 -0.15 -0.09 -0.01

WT3RAP60RAP140RAP220RAP300RAP380RAP460RAP620RAP70002468WT3HU80HU120HU16002468WT3rpd3Δ_1rpd3Δ_2rpd3Δ_302468WT1AF100AF140AF18002468
rapamycinhydroxy-urearpd3 knockoutalpha-factorMultiple Condition Time-Course Intensity PlotsGFP Intensity (1e-4)GFP Intensity (1e-4)GFP Intensity (1e-4)GFP Intensity (1e-4)

Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – 0 0
Bud Neck – – – – – – – – – – – – – 0 0
Bud Site – – – – – – – – – – – – – 0 0
Cell Periphery – – – – – – – – – – – – – 0 0
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – 0 0
Endosome – – – – – – – – – – – – – 0 0
Golgi – – – – – – – – – – – – – 0 0
Mitochondria – – – – – – – – – – – – – 0 0
Nuclear Periphery – – – – – – – – – – – – – 0 0
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – 0 0
Peroxisomes – – – – – – – – – – – – – 0 0
SpindlePole – – – – – – – – – – – – – 0 0
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – 0 0
Cytoplasm – – – – – – – – – – – – – 0 0
Nucleus – – – – – – – – – – – – – 0 0
Vacuole – – – – – – – – – – – – – 3.0 2.2

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 1.8199 1.9261 1.7328 1.6858 1.3279 1.9263 -0.0656 -0.0923 -0.3914 -0.2401 -0.2088 -0.3257 -0.1268 -0.1069 -0.2661 0.121 -0.484 -0.1912
Actin 0.0235 0.0033 0.0073 0.0014 0.0013 0.0019 0.0157 0.0006 0.004 0.0066 0.0028 0.001 0.0007 0.0001 0.0006 0.0003 0.0001 0.0001
Bud 0.0003 0.0001 0.0001 0.0001 0.0001 0.0002 0.0004 0.0001 0.0003 0.0001 0.0009 0.0001 0.0001 0.0001 0 0 0 0
Bud Neck 0.0114 0.0013 0.0014 0.0017 0.0032 0.0152 0.0114 0.0016 0.0009 0.001 0.0032 0.006 0.0008 0.0007 0.0007 0.001 0.0008 0.0026
Bud Periphery 0.0004 0.0002 0.0002 0.0001 0.0002 0.0005 0.0009 0.0004 0.0006 0.0001 0.0018 0.0001 0.0001 0 0.0001 0 0 0
Bud Site 0.0098 0.0016 0.0008 0.0005 0.0004 0.0007 0.0018 0.0008 0.0014 0.0005 0.0119 0.0001 0.0003 0.0004 0.0002 0.0001 0 0
Cell Periphery 0.0004 0.0001 0.0001 0.0001 0.0001 0.0007 0.0002 0.0009 0.0001 0.0001 0.0002 0 0.0001 0 0 0 0 0
Cytoplasm 0.0341 0.0178 0.0104 0.0324 0.0109 0.0189 0.0097 0.0091 0.0083 0.004 0.0079 0.0021 0.0046 0.0049 0.0051 0.0032 0.0011 0.0021
Cytoplasmic Foci 0.0167 0.0063 0.0065 0.0029 0.0071 0.0072 0.0128 0.0003 0.003 0.0086 0.0077 0.0017 0.0033 0.0003 0.0004 0.0003 0.0002 0.0005
Eisosomes 0.0015 0.0002 0.0007 0.0001 0.0001 0.0003 0.0003 0 0 0.0001 0.0001 0 0.0001 0 0 0 0 0
Endoplasmic Reticulum 0.005 0.0017 0.001 0.0031 0.0009 0.0011 0.0022 0.001 0.0008 0.0007 0.001 0.0001 0.0008 0.0002 0.0004 0.0002 0.0001 0.0001
Endosome 0.0163 0.005 0.0065 0.0038 0.0077 0.0036 0.0157 0.0019 0.0072 0.0097 0.0258 0.0021 0.0017 0.0002 0.0008 0.0004 0.0003 0.0002
Golgi 0.0052 0.001 0.0024 0.0002 0.002 0.003 0.0081 0.0002 0.0018 0.0043 0.0373 0.0016 0.0002 0 0.0002 0 0 0
Lipid Particles 0.0204 0.009 0.0083 0.0005 0.0267 0.0066 0.0326 0.0005 0.005 0.0033 0.0252 0.0032 0.0011 0 0.0007 0.0001 0 0.0001
Mitochondria 0.0119 0.0017 0.0111 0.0008 0.0026 0.0104 0.0284 0.0013 0.0029 0.0008 0.0191 0.0046 0.0022 0.0001 0.0014 0.0002 0 0
None 0.0923 0.0939 0.0829 0.0521 0.056 0.0828 0.0047 0.014 0.0128 0.0032 0.0029 0.0009 0.012 0.0123 0.006 0.0021 0.0004 0.0024
Nuclear Periphery 0.0385 0.0346 0.0671 0.0309 0.0393 0.0041 0.0289 0.0951 0.0671 0.0166 0.0422 0.0113 0.0153 0.0105 0.0193 0.0282 0.0355 0.0039
Nucleolus 0.0389 0.0443 0.0427 0.0331 0.0673 0.0586 0.037 0.0487 0.0438 0.0403 0.0375 0.0756 0.0254 0.026 0.0314 0.0222 0.0212 0.0428
Nucleus 0.5331 0.6491 0.6039 0.5473 0.5754 0.6985 0.7001 0.7304 0.7464 0.8465 0.6322 0.8336 0.6695 0.6053 0.5943 0.4941 0.5598 0.6202
Peroxisomes 0.0173 0.0065 0.0109 0.0003 0.0173 0.004 0.0216 0.0001 0.0039 0.0009 0.0056 0.0017 0.0002 0 0.0002 0 0 0
Punctate Nuclear 0.1199 0.12 0.1334 0.2874 0.1795 0.08 0.0637 0.0841 0.0872 0.0518 0.1261 0.0497 0.2601 0.3386 0.3378 0.4473 0.3801 0.3249
Vacuole 0.0023 0.0014 0.0009 0.0007 0.0009 0.0013 0.0022 0.0073 0.0015 0.0004 0.0073 0.0034 0.0007 0.0001 0.0002 0.0002 0.0001 0
Vacuole Periphery 0.0007 0.0009 0.0013 0.0003 0.001 0.0003 0.0014 0.0016 0.001 0.0003 0.0011 0.0011 0.0006 0.0001 0.0002 0.0001 0.0001 0

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 12.8154 5.3462 4.5339 9.0573 8.8617 8.1119 8.6875 8.7301 7.6177 11.8097
Translational Efficiency 0.3537 0.8267 0.9361 0.3731 0.433 0.4996 0.5692 0.3817 0.5696 0.3778

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1510 332 228 1638 1473 1911 565 66 2983 2243 793 1704

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 668.57 737.65 1043.83 896.13 617.28 761.52 929.91 906.30 643.24 757.99 962.66 896.52
Standard Deviation 73.47 109.45 140.65 117.98 94.11 99.06 106.89 117.00 88.11 101.02 128.40 117.96
Intensity Change Log 2 — 0.141858 0.642736 0.422629 — 0.302957 0.591166 0.554064 — 0.221442 0.618210 0.487221

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000133 0.002372 0.004484 0.001317 0.000379 0.000477 0.001669 0.006515 0.000254 0.000757 0.002478 0.001518
Bud Neck 0.003308 0.011041 0.016457 0.027110 0.003574 0.009493 0.016514 0.026185 0.003439 0.009723 0.016498 0.027075
Bud Site 0.000805 0.010281 0.004831 0.012450 0.001001 0.003175 0.003864 0.156894 0.000902 0.004227 0.004142 0.018044
Cell Periphery 0.000126 0.000216 0.000165 0.000146 0.000164 0.000127 0.000090 0.000372 0.000145 0.000140 0.000111 0.000155
Cytoplasm 0.194562 0.079473 0.003733 0.039550 0.117143 0.106550 0.014938 0.051006 0.156333 0.102542 0.011716 0.039994
Cytoplasmic Foci 0.064209 0.044404 0.000416 0.001214 0.028068 0.040859 0.000213 0.006975 0.046362 0.041384 0.000272 0.001437
Eisosomes 0.000070 0.000113 0.000103 0.000037 0.000061 0.000072 0.000054 0.000112 0.000065 0.000078 0.000068 0.000040
Endoplasmic Reticulum 0.001601 0.008617 0.020481 0.009980 0.002785 0.003638 0.009465 0.026689 0.002186 0.004375 0.012632 0.010628
Endosome 0.003675 0.009203 0.001169 0.003037 0.002307 0.004985 0.000903 0.003785 0.003000 0.005609 0.000979 0.003066
Golgi 0.001204 0.005069 0.000417 0.002766 0.001090 0.002466 0.000197 0.000381 0.001148 0.002851 0.000260 0.002673
Lipid Particles 0.019308 0.012794 0.001471 0.000741 0.009151 0.016876 0.000754 0.003792 0.014293 0.016272 0.000960 0.000860
Mitochondria 0.009102 0.008013 0.002269 0.010426 0.008052 0.020264 0.001856 0.009861 0.008584 0.018450 0.001975 0.010404
Mitotic Spindle 0.008672 0.039246 0.009461 0.083354 0.006893 0.040966 0.014214 0.132976 0.007794 0.040712 0.012848 0.085276
None 0.030761 0.015688 0.002689 0.008464 0.052746 0.014533 0.006961 0.006767 0.041617 0.014704 0.005733 0.008399
Nuclear Periphery 0.008117 0.006303 0.002113 0.008229 0.004116 0.010895 0.002319 0.008219 0.006141 0.010215 0.002260 0.008229
Nuclear Periphery Foci 0.011435 0.019013 0.008402 0.002855 0.010692 0.009282 0.002647 0.015737 0.011068 0.010722 0.004302 0.003354
Nucleolus 0.064740 0.060206 0.027445 0.013441 0.077722 0.074717 0.029658 0.015794 0.071150 0.072569 0.029022 0.013532
Nucleus 0.569273 0.643138 0.881347 0.753058 0.667456 0.625895 0.883997 0.456765 0.617755 0.628447 0.883235 0.741582
Peroxisomes 0.003752 0.013525 0.001676 0.000648 0.002458 0.007365 0.000521 0.003572 0.003113 0.008277 0.000853 0.000761
Vacuole 0.003903 0.009820 0.010445 0.017661 0.003205 0.006021 0.008896 0.066331 0.003558 0.006583 0.009341 0.019546
Vacuole Periphery 0.001244 0.001464 0.000425 0.003516 0.000938 0.001345 0.000269 0.001271 0.001093 0.001363 0.000314 0.003429

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -3.92 -15.61 -23.16 1.14 10.21 -1.37 -13.42 -2.45 -2.42 -1.58 -5.37 -20.12 -14.43 -7.85 6.92
Bud Neck -5.05 -9.68 -20.39 -10.69 -1.23 -8.27 -11.27 -4.28 -3.32 -1.14 -10.09 -14.78 -20.78 -15.23 -4.10
Bud Site -3.09 -5.56 -10.07 -0.68 -1.06 -3.10 -7.27 -3.50 -3.42 -3.31 -4.68 -9.28 -9.23 -6.58 -4.78
Cell Periphery -3.65 -4.53 -0.91 3.34 4.09 3.80 4.91 -2.22 -2.99 -3.23 0.77 0.85 0.81 0.04 -0.05
Cytoplasm 10.92 30.04 24.37 5.65 -14.17 1.93 20.42 8.71 7.83 -3.68 9.59 34.74 27.34 17.20 -11.42
Cytoplasmic Foci 4.46 20.53 20.49 7.40 -1.17 -3.98 14.01 11.25 15.99 -2.03 2.91 24.44 24.26 20.49 -3.67
Eisosomes -4.38 -4.10 7.96 7.20 7.99 -2.85 -1.17 -3.27 -2.49 -2.72 -3.50 -2.46 6.77 9.03 6.68
Endoplasmic Reticulum -6.82 -15.30 -26.91 -3.48 5.04 -2.85 -14.30 -6.16 -5.97 -3.45 -8.79 -20.76 -25.76 -18.94 1.34
Endosome -1.86 6.91 5.85 2.87 -1.66 -5.06 4.99 -2.43 0.67 -4.02 -4.03 8.88 4.91 6.49 -3.70
Golgi -1.91 2.69 0.89 2.11 -0.58 -2.74 4.76 4.18 5.34 -0.73 -3.43 5.69 1.07 3.50 -1.21
Lipid Particles 3.22 14.40 15.00 5.81 3.50 -5.94 10.29 5.70 11.14 -3.82 -0.93 17.69 17.81 16.26 0.67
Mitochondria 1.07 6.47 1.90 0.44 -5.16 -6.23 4.52 0.74 6.13 -2.48 -6.31 7.72 1.63 7.54 -5.77
Mitotic Spindle -4.65 -2.41 -13.38 -3.16 -5.83 -10.38 -5.05 -4.73 -3.65 -3.98 -11.56 -5.55 -14.45 -6.15 -7.95
None 6.99 16.73 13.98 5.07 -7.75 12.18 15.38 15.75 8.49 2.20 14.39 21.02 20.17 9.14 -1.94
Nuclear Periphery 1.18 6.75 -3.88 -3.53 -12.59 -8.10 -0.58 -3.05 -0.50 -2.92 -5.38 3.89 -7.40 -1.79 -10.97
Nuclear Periphery Foci -2.02 3.21 7.55 4.78 1.62 0.91 4.80 0.06 -0.20 -1.28 0.19 6.18 9.26 8.32 1.64
Nucleolus 2.83 8.18 17.23 8.31 4.54 -0.25 7.78 16.15 18.34 6.92 0.46 10.73 23.70 22.45 7.76
Nucleus -4.29 -19.24 -18.92 -6.40 5.77 4.61 -14.65 4.11 2.96 8.22 -0.97 -24.74 -15.19 -13.40 9.16
Peroxisomes -2.95 5.53 11.12 4.49 6.63 -7.22 7.32 2.44 8.18 -1.93 -7.17 10.62 13.07 12.76 5.24
Vacuole -3.62 -7.11 -12.53 -6.20 -5.87 -4.60 -5.27 -4.61 -4.45 -4.01 -5.71 -7.16 -13.46 -11.51 -7.02
Vacuole Periphery -0.53 1.39 -0.31 0.22 -2.07 -1.27 3.04 -0.19 1.04 -3.20 -1.04 3.04 -0.61 0.15 -2.87
Protein Complexes26S Proteasome complex37S mitochondrial small ribosomal subunit54S mitochondrial large ribosomal subunit60S cytosolic large ribosomal subunitAar2-U5 snRNPAnaphase-Promoting Complex variant 1Anaphase-Promoting Complex variant 2Anaphase-Promoting Complex variant 3Anaphase-Promoting Complex variant 4Arp2/3 complexATG1 kinase complexBLOC-1 complexCcr4-Not complexCentral kinetochore CTF19 complexCleavage and polyadenylation specificity factorcomplexCMG helicase complexCMG-Pol epsilon complexCOMPASS complexCOP9 signalosome COPI vesicle coat complexCoQ6 biosynthesis complexCore mediator complexCORVET complexCTF18-RFC complexCURI complex variant 1CURI complex variant 2CURI complex variant 3Cytoplasmic exosome complex, DIS3 variantDASH complexDNA-directed RNA Polymerase I complexDNA-directed RNA polymerase II complexDNA-directed RNA polymerase III complexDNA replication factor C complexEGO complexELG1-RFC complex Elongator holoenzyme complexEndoplasmic Reticulum Membrane ComplexEukaryotic translation initiation factor 2BcomplexEukaryotic translation initiation factor 3core complexExocystExomer complexFAR complexGeneral transcription factor complex TFIIDGeneral transcription factor complex TFIIHGET4-GET5 transmembrane domain recognition complexGID ubiquitin ligase complexGin4 complexGolgi transport complexGSE complexHMC complexHOPS complexINO80 chromatin remodeling complexLSM1-7 complexLsm1-7-Pat1 complexLSM2-7 complexLSM2-8 complexMCM complexMICOS complexMitochondrial electron transport complex IIIMitochondrial inner membrane pre-sequence translocasecomplexMitochondrial outer membrane translocase holocomplexMitochondrial proton-transporting ATP synthase complexMitochondrial pyruvate dehydrogenase complexNineTeen complexNOP8 60s ribosome pre-assembly complexNuA3 histone acetyltransferase complexNuA4 histone acetyltransferase complexNuclear condensin complexNuclear/nucleolar exosome complex, DIS3-RRP6 variantNuclear pore complexNucleolar ribonuclease MRP complexNucleolar ribonuclease P complexOligosaccharyl transferase complex variant 1Oligosaccharyl transferase complex variant 2PAF1 complexPAS complexPhosphatidylinositol 3-kinase complex IPolarisomePrefoldin complexPRP19-associated complexRad17 RFC-like complexRetromer complexRPD3L histone deacetylase complexRPD3S histone deacetylase complexRQC complexRSC complex variant RSC1RSC complex variant RSC2SAGA complexSCF-Met30 ubiquitin ligase complexSCF-Ufo1 ubiquitin ligase complexSEA complexSeptin complexSET3C histone deacetylase complexSignal recognition particleSLIK (SAGA-like) complexSmall ribosomal subunit processome, variant1Small ribosomal subunit processome, variant2Small ribosomal subunit processome, variant3SMC5-SMC6 SUMO ligase complexSm complexSpliceosomal commitment complexSPOTS complexSWI/SNF chromatin remodelling complexSwr1 chromatin remodelling complexTHO complexTranscription factor TFIIIC complexTranslocon complexTRAPPIII protein complexTRAPPII protein complexTRAPPI protein complexTREX complexU1 snRNPU2 snRNPU4 snRNPU4/U6 snRNPU4/U6.U5 tri-snRNP complexU5 snRNPU6 snRNP complexUTP-A complexUTP-B complexUTP-C complex variant 1UTP-C complex variant 2UTP-C complex variant 3Vacuolar proton translocating ATPase complex,Golgi variantVacuolar proton translocating ATPase complex,vacuole variantCellular Components••••••••••••••••actinbud neckcell peripherycytoplasmendosomeERGolgimitochondrionmixednuclear peripherynucleolusnucleusperoxisomespindle polevacuolar membranevacuole
Standard name
Human Ortholog
Description Protein proposed to be involved in vacuolar functions; mutant shows defect in CPY processing and fragmented vacuoles; deletion mutant has increased glycogen accumulation and displays elongated buds; green fluorescent protein (GFP)-fusion protein localizes to the nucleus; ENV11 has a paralog, VID22, that arose from the whole genome duplication
Localization
Cell Percentages nucleus (79%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-3

Env11

Env11


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Env11-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
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