Standard name
Human Ortholog
Description Mitochondrial ribosomal protein of the large subunit; mutation confers increased replicative lifespan

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0.05 0.05 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0.05 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0.12 0.16 0.06 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.06 0.07 0.05 0.14 0.05 0 0 0.08 0 0.06
Golgi 0 0 0 0 0 0 0 0 0 0 0 0.1 0.07 0.1 0.33 0.05 0.25 0.08 0.1 0.06 0.08 0.1 0.09
Mitochondria 0.94 0.87 0.97 0.96 0.85 0.91 0.86 0.92 0.9 0.93 0.95 0.9 0.87 0.82 0 0.05 0.14 0.72 0.69 0.79 0.67 0.65 0.62
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0.06 0 0.06 0.09 0.09 0.09 0.07 0.09 0.08 0.06 0 0 0.1 0.05 0 0 0 0 0 0 0
Nucleolus 0 0 0 0.06 0.07 0.1 0.13 0.08 0.07 0.09 0.1 0 0 0 0.1 0.11 0.11 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0.09 0.07 0.37 0.17 0 0 0.07 0.08 0.1 0.08
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.08 0 0 0 0 0 0
Vac/Vac Membrane 0.14 0.22 0.08 0 0.08 0.07 0 0 0.05 0 0 0.09 0.09 0.13 0.19 0.05 0.19 0.06 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 1 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 3 5 0 6 13 11
Bud 4 0 5 1 3 3 4 9 8 6 8 4 4 2 2 1 0 0 0 0 0 2 3
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 3 0
Bud Site 0 0 0 1 0 2 2 1 2 4 4 1 1 0 0 0 0
Cell Periphery 0 2 2 0 2 1 1 1 5 2 2 9 7 16 3 1 0 0 1 0 0 0 0
Cytoplasm 4 3 3 1 8 3 2 5 6 1 2 5 9 17 5 3 2 3 0 1 0 1 1
Endoplasmic Reticulum 3 2 3 2 2 1 0 0 0 3 1 5 1 1 1 0 3 0 0 0 0 1 3
Endosome 1 6 0 2 5 0 3 0 1 2 0 16 23 27 3 1 5 9 6 4 17 11 24
Golgi 6 4 2 0 1 0 0 0 0 0 0 37 25 45 14 1 9 17 14 6 19 37 34
Mitochondria 198 127 106 78 134 160 173 207 257 207 269 345 309 373 1 1 5 154 99 89 157 252 229
Nucleus 1 0 1 0 1 2 4 3 4 1 2 0 1 0 0 0 1 0 0 0 0 1 1
Nuclear Periphery 6 4 6 2 9 16 17 21 20 20 24 22 10 7 4 1 1 1 2 0 2 2 7
Nucleolus 8 2 3 5 11 18 26 19 20 21 28 8 8 6 4 2 4 0 0 0 2 3 3
Peroxisomes 2 3 0 0 6 3 3 3 5 3 2 14 24 41 3 7 6 4 4 8 17 39 28
SpindlePole 1 1 1 0 3 0 5 5 10 2 4 8 1 5 0 1 3 0 1 0 1 3 4
Vac/Vac Membrane 30 32 9 0 12 12 6 7 14 6 9 33 32 60 8 1 7 12 6 1 5 8 14
Unique Cell Count 210 146 109 81 158 175 200 225 287 223 283 384 356 454 42 19 36 214 144 113 236 390 372
Labelled Cell Count 265 186 141 92 197 221 246 281 352 278 355 508 455 600 48 20 46 214 144 113 236 390 372


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 7.5 8.6 6.1 7.0 7.6 6.3 6.0 5.6 5.7 5.9 5.4 8.0 8.1 8.2 9.7 9.7 9.1 8.0 8.4 8.3
Std Deviation (1e-4) 1.4 1.6 1.5 3.3 1.8 1.9 1.6 2.0 1.6 2.2 1.5 1.6 1.7 1.8 2.2 2.0 2.3 2.0 2.0 1.9
Intensity Change (Log2) 0.18 0.3 0.03 -0.04 -0.13 -0.11 -0.06 -0.17 0.39 0.41 0.42 0.66 0.66 0.56 0.38 0.45 0.44


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 2.7 2.6 0 0 0
Golgi 0 0 0 0 0 0 0 0 2.7 2.0 2.7 0 0 0
Mitochondria -0.4 -3.3 -2.0 -3.0 -1.9 -2.5 -1.6 -1.0 -2.4 -3.1 -4.0 -11.5 -10.0 -10.2
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0.1 1.1 1.0 1.2 0.5 1.1 1.0 0.1 0 0 0 0 0
Nucleolus 0 1.5 2.4 3.0 2.0 1.6 2.2 2.3 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 2.8 3.3 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 -0.2 -0.4 -2.1 -2.1 -1.3 0 -2.2 0.1 0.2 1.4 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 3.7144 3.9227 3.5777 3.0508 3.1432 3.5206 3.5289 3.6978 3.16 3.5372 2.9257 3.3437 4.4193 4.2878 4.2169 4.3901 3.3769 4.4154
Actin 0.0464 0.0245 0.0163 0.0677 0.0266 0.0051 0.0131 0.0088 0.0138 0.0021 0.01 0.0047 0.0744 0.0214 0.0266 0.0039 0.0545 0.0014
Bud 0.0004 0.0009 0.001 0.0006 0.0001 0.0002 0.0006 0.0009 0.0005 0.0005 0.0005 0.0003 0.0009 0.0011 0.0006 0.0094 0.0004 0
Bud Neck 0.0007 0.0003 0.0006 0.0003 0.0002 0.0001 0.0004 0.0002 0.0005 0.0001 0.0002 0.0003 0.0016 0.0007 0.0005 0.0002 0.0001 0.0002
Bud Periphery 0.0007 0.0016 0.0022 0.0011 0.0003 0.0003 0.0018 0.0017 0.0008 0.001 0.0009 0.0004 0.0013 0.0018 0.0016 0.0407 0.0008 0.0001
Bud Site 0.0015 0.0132 0.0126 0.0016 0.0003 0.0004 0.001 0.0064 0.0019 0.0006 0.0012 0.0007 0.0064 0.012 0.0022 0.0014 0.0004 0.0001
Cell Periphery 0.0003 0.0002 0.0009 0.0003 0.0001 0.0001 0.0003 0.0003 0.0001 0.0001 0.0001 0.0001 0.0003 0.0009 0.0008 0.0002 0.0002 0.0001
Cytoplasm 0.0022 0.0012 0.0044 0.0006 0.0001 0.0002 0.0028 0.0008 0.0005 0.0001 0.0007 0.0002 0.0012 0.0026 0.0009 0.0002 0.0001 0.0199
Cytoplasmic Foci 0.013 0.015 0.0166 0.0157 0.0096 0.003 0.02 0.0108 0.015 0.0038 0.0117 0.0059 0.0259 0.0124 0.012 0.0262 0.0026 0.0033
Eisosomes 0.001 0.0002 0.0007 0.0007 0.0001 0.0001 0.001 0.0003 0.0006 0.0001 0.0002 0.0003 0.0008 0.0005 0.0006 0.0002 0.0003 0.0001
Endoplasmic Reticulum 0.0021 0.0001 0.0008 0.0021 0 0.0001 0.003 0.0003 0.0002 0.0001 0.0008 0.0005 0.0015 0.0009 0.0021 0.0001 0 0.0003
Endosome 0.0332 0.0147 0.0277 0.0365 0.0091 0.006 0.0547 0.0277 0.0164 0.011 0.0472 0.0148 0.0317 0.0273 0.0154 0.0108 0.0412 0.0075
Golgi 0.0291 0.0295 0.0362 0.0309 0.0293 0.018 0.0499 0.0452 0.0328 0.0085 0.0283 0.0343 0.0368 0.0449 0.023 0.0189 0.0117 0.0244
Lipid Particles 0.0489 0.0272 0.0107 0.0151 0.0052 0.0032 0.0272 0.0084 0.0077 0.0076 0.0193 0.0091 0.0441 0.0199 0.0082 0.0485 0.0197 0.0187
Mitochondria 0.7688 0.8157 0.8307 0.7971 0.8806 0.9548 0.7439 0.8592 0.8811 0.9382 0.836 0.897 0.6874 0.7969 0.8405 0.7497 0.8223 0.9006
None 0.0109 0.0003 0.0045 0.0007 0.0001 0.0002 0.0057 0.0002 0.0004 0.0001 0.0009 0.0003 0.0032 0.0017 0.0006 0.0005 0.0001 0.0001
Nuclear Periphery 0.0044 0.0001 0.0005 0.0017 0 0.0001 0.0158 0.0001 0.0002 0 0.0067 0.0033 0.0018 0.0006 0.0002 0.0001 0 0.0015
Nucleolus 0.0013 0.0008 0.0003 0.0001 0 0.0001 0.0019 0.0006 0.0001 0.0001 0.0005 0.0003 0.0007 0.0004 0.0001 0.0003 0.0001 0.0001
Nucleus 0.0026 0.0003 0.0006 0.0002 0 0.0002 0.0075 0.0002 0.0001 0.0001 0.0012 0.001 0.0004 0.0002 0.0001 0.0002 0 0.0101
Peroxisomes 0.0242 0.0507 0.0281 0.0191 0.0369 0.0058 0.0331 0.0223 0.0251 0.0232 0.0239 0.0234 0.0748 0.05 0.061 0.0869 0.0392 0.0097
Punctate Nuclear 0.0023 0.0001 0.0008 0.0008 0.0002 0.0002 0.0089 0.0001 0.0005 0.0001 0.0026 0.0007 0.0015 0.0004 0.0002 0.0006 0 0.0003
Vacuole 0.0024 0.0022 0.0018 0.0037 0.0003 0.0005 0.0033 0.0031 0.0007 0.0013 0.0043 0.0007 0.0015 0.0017 0.0014 0.0008 0.0032 0.0004
Vacuole Periphery 0.0037 0.0012 0.0019 0.0034 0.0008 0.0012 0.0039 0.0025 0.0008 0.0013 0.0027 0.0017 0.0017 0.0018 0.0014 0.0006 0.003 0.0011

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 16.295 20.8096 18.53 18.2995 9.0545 18.6126 19.9886 25.5618 24.4622 25.2471
Translational Efficiency 2.3757 1.5476 1.9842 2.0398 3.8992 3.2664 2.5738 1.9792 2.3667 2.4531

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1426 1239 219 1859 1699 1889 1925 1804 3125 3128 2144 3663

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 681.08 870.58 1269.53 1069.10 807.39 897.76 1064.95 1051.17 749.75 886.99 1085.85 1060.27
Standard Deviation 109.44 152.58 191.36 183.74 121.92 140.68 150.99 167.35 132.31 146.12 167.47 176.09
Intensity Change Log 2 0.354153 0.898398 0.650501 0.153064 0.399448 0.380658 0.248566 0.649271 0.510418

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.001130 0.003687 0.002497 0.005622 0.001723 0.001452 0.004255 0.007653 0.001452 0.002337 0.004076 0.006623
Bud Neck 0.005683 0.002181 0.006555 0.003550 0.001138 0.001585 0.002090 0.001961 0.003212 0.001821 0.002546 0.002768
Bud Site 0.010618 0.008185 0.005242 0.017522 0.002850 0.003758 0.009177 0.015002 0.006395 0.005512 0.008775 0.016281
Cell Periphery 0.001046 0.000376 0.000170 0.000528 0.000568 0.000242 0.000167 0.000824 0.000786 0.000295 0.000168 0.000674
Cytoplasm 0.006063 0.001709 0.000802 0.003401 0.000637 0.001005 0.001561 0.002861 0.003113 0.001284 0.001484 0.003135
Cytoplasmic Foci 0.021646 0.026975 0.066464 0.019886 0.011320 0.017212 0.031060 0.017507 0.016032 0.021079 0.034677 0.018714
Eisosomes 0.000073 0.000093 0.000068 0.000071 0.000053 0.000060 0.000059 0.000089 0.000062 0.000073 0.000060 0.000080
Endoplasmic Reticulum 0.000418 0.000293 0.000935 0.000622 0.000155 0.000187 0.000375 0.000472 0.000275 0.000229 0.000432 0.000548
Endosome 0.064295 0.041157 0.047994 0.035436 0.025891 0.030283 0.038010 0.029827 0.043415 0.034590 0.039029 0.032673
Golgi 0.042578 0.038215 0.062878 0.048718 0.020663 0.028292 0.046493 0.042445 0.030663 0.032222 0.048167 0.045629
Lipid Particles 0.006995 0.008059 0.010941 0.001968 0.004776 0.004939 0.001330 0.002202 0.005788 0.006175 0.002312 0.002083
Mitochondria 0.779986* 0.804392* 0.649436* 0.781841* 0.901069* 0.873221* 0.779961* 0.819246* 0.845816* 0.845958* 0.766628* 0.800262*
Mitotic Spindle 0.002402 0.006515 0.055448 0.022801 0.001614 0.002950 0.032667 0.011540 0.001974 0.004362 0.034994 0.017255
None 0.001900 0.000336 0.000663 0.000605 0.000177 0.000331 0.000241 0.000289 0.000963 0.000333 0.000284 0.000449
Nuclear Periphery 0.000062 0.000082 0.005282 0.000746 0.000051 0.000050 0.000461 0.000376 0.000056 0.000063 0.000953 0.000564
Nuclear Periphery Foci 0.000652 0.000158 0.004229 0.000570 0.000126 0.000211 0.000384 0.000437 0.000366 0.000190 0.000777 0.000504
Nucleolus 0.001281 0.000199 0.001655 0.000476 0.000069 0.000402 0.000142 0.000158 0.000622 0.000322 0.000297 0.000320
Nucleus 0.001902 0.000211 0.001218 0.000450 0.000075 0.000253 0.000179 0.000192 0.000909 0.000236 0.000285 0.000323
Peroxisomes 0.037136 0.047924 0.017259 0.015419 0.022497 0.027532 0.026051 0.022696 0.029177 0.035610 0.025153 0.019003
Vacuole 0.006757 0.005073 0.026071 0.019490 0.001796 0.003120 0.012205 0.014232 0.004060 0.003893 0.013621 0.016901
Vacuole Periphery 0.007375 0.004182 0.034192 0.020278 0.002755 0.002917 0.013130 0.009992 0.004863 0.003418 0.015282 0.015212

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -4.65 -2.18 -4.41 -1.68 -2.63 0.85 -3.84 -5.46 -5.81 -2.78 -3.14 -4.61 -6.93 -5.58 -2.76
Bud Neck 3.33 -0.65 1.77 -1.85 1.92 -0.98 -2.72 -2.05 -0.87 0.49 2.57 1.08 0.71 -2.18 -0.36
Bud Site 1.13 2.56 -3.13 -4.10 -5.62 -0.86 -5.09 -7.47 -6.84 -3.16 0.81 -2.07 -7.28 -7.96 -5.03
Cell Periphery 3.36 4.56 2.37 -1.27 -3.35 1.58 1.97 -0.75 -2.08 -2.37 3.40 4.38 0.55 -2.53 -3.44
Cytoplasm 3.33 4.13 1.81 -2.79 -4.79 -1.70 -4.37 -4.98 -3.80 -2.79 2.95 2.70 -0.12 -4.73 -4.52
Cytoplasmic Foci -1.92 -5.41 0.95 2.88 5.69 -3.93 -10.00 -3.95 -0.20 6.21 -3.50 -9.26 -2.00 1.60 7.88
Eisosomes -2.85 0.72 0.57 3.24 -0.34 -1.52 -1.65 -4.91 -3.93 -4.11 -2.76 0.62 -3.91 -1.42 -4.30
Endoplasmic Reticulum 1.87 -1.73 -1.70 -2.82 1.01 -1.02 -5.12 -3.08 -2.69 -0.88 1.30 -3.00 -3.49 -4.07 -1.29
Endosome 5.46 2.59 7.43 1.74 2.33 -1.73 -4.67 -1.55 0.18 3.20 3.69 1.70 4.68 0.94 2.92
Golgi 1.21 -2.32 -1.62 -2.77 1.61 -3.28 -9.02 -7.38 -4.63 1.16 -0.75 -6.38 -6.33 -5.63 0.85
Lipid Particles -0.59 -1.47 4.12 4.53 3.72 -0.17 3.61 2.42 2.74 -1.61 -0.39 4.35 4.65 5.15 0.49
Mitochondria -2.11 5.10 -0.14 2.12 -5.23 3.70 13.81 9.55 6.11 -3.99 -0.04 9.42 6.60 6.68 -3.96
Mitotic Spindle -3.07 -5.51 -9.87 -6.73 3.33 -2.60 -13.39 -8.28 -6.99 8.24 -3.93 -14.38 -12.64 -9.76 6.86
None 3.20 2.55 2.48 -1.34 0.23 -1.50 -2.26 -3.26 0.44 -2.08 2.73 3.06 2.12 -0.98 -1.66
Nuclear Periphery -0.94 -3.04 -6.02 -5.81 2.63 0.01 -3.57 -2.03 -2.04 0.42 -0.61 -4.37 -5.19 -5.12 1.70
Nuclear Periphery Foci 2.72 -1.97 0.51 -4.74 2.03 -1.62 -3.63 -1.71 -1.16 -0.27 1.94 -1.86 -0.96 -3.02 1.24
Nucleolus 2.87 -0.39 2.00 -1.80 1.18 -2.65 -2.03 -1.21 1.71 -0.21 1.62 1.58 1.59 -0.03 -0.14
Nucleus 2.43 0.63 2.00 -1.99 1.14 -2.16 -2.26 -1.70 0.52 0.06 2.11 1.82 1.73 -1.27 -0.29
Peroxisomes -2.25 3.66 6.38 7.93 0.39 -1.68 -1.25 -0.09 1.61 1.18 -2.42 1.48 4.52 6.94 2.69
Vacuole 1.74 -5.52 -8.20 -9.31 1.88 -2.92 -11.35 -8.49 -7.43 -1.17 0.34 -10.23 -12.10 -12.20 -2.35
Vacuole Periphery 3.41 -5.94 -8.25 -11.35 2.99 -0.39 -9.90 -8.10 -7.85 2.38 3.01 -9.49 -11.54 -13.98 0.04
Standard name
Human Ortholog
Description Mitochondrial ribosomal protein of the large subunit; mutation confers increased replicative lifespan
Localization
Cell Percentages mitochondrion (97%)
Cell Cycle Regulation No
Subcompartmental Group mito-2

Mrpl25

Mrpl25


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Mrpl25-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available