Standard name
Human Ortholog
Description Part of the heteromeric co-chaperone GimC/prefoldin complex; complex promotes efficient protein folding
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0.06 – 0 0.07 0.05 0.07 0 0 0 0 0.05 0.05 0 0 0 0 0 0
Cytoplasm 0.96 0.99 0.98 0.98 0.88 0.89 0.83 – 0.8 0.76 0.72 0.76 1.0 0.99 0.99 0.96 0.98 0.98 0.92 0.97 0.97 0.95 0.97 0.93
Endoplasmic Reticulum 0 0 0 0 0 0 0 – 0 0 0 0.07 0 0 0 0 0 0.05 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.12 0 – 0.15 0.15 0.17 0.13 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0.08 0 0.12 – 0.06 0.11 0.11 0.07 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 1 1 6 – 5 5 5 6 0 0 0 0 0 0 0 0 0 0 0 0
Bud 1 1 0 0 0 5 1 – 5 8 2 2 0 0 0 0 0 0 1 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 3 0 4 7 11 5 30 – 17 29 17 31 6 13 8 4 7 7 0 0 0 0 0 0
Cytoplasm 240 241 301 252 269 277 449 – 344 322 239 329 262 369 340 110 134 137 265 238 292 193 253 245
Endoplasmic Reticulum 5 2 1 0 0 3 16 – 8 11 6 30 1 4 1 3 2 7 2 1 0 2 2 3
Endosome 1 0 0 0 1 0 0 – 1 2 0 1 0 0 0 0 0 0 2 0 0 0 0 0
Golgi 0 0 0 1 4 0 0 – 1 0 1 0 0 1 0 1 0 2 1 0 0 0 1 0
Mitochondria 0 0 2 2 6 39 11 – 64 64 57 56 0 0 1 4 1 1 1 0 1 1 0 1
Nucleus 5 0 3 0 0 4 1 – 3 1 2 1 1 0 0 0 0 1 3 1 0 0 0 0
Nuclear Periphery 1 0 0 0 0 0 0 – 0 0 1 1 0 0 0 0 0 0 0 0 1 0 0 1
Nucleolus 0 0 0 0 0 0 0 – 0 0 0 0 0 1 0 0 0 0 0 0 0 1 0 1
Peroxisomes 0 0 0 0 0 0 0 – 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 1 0 1 – 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 6 0 1 2 25 14 63 – 25 45 36 29 1 0 2 3 2 0 2 1 2 0 0 4
Unique Cell Count 250 243 307 257 305 312 542 428 426 330 431 263 374 344 115 137 140 287 245 300 203 260 264
Labelled Cell Count 262 244 312 264 318 348 578 473 487 367 487 271 388 352 125 146 155 287 245 300 203 260 264


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 14.6 13.7 12.5 12.3 12.3 8.1 10.3 – 9.1 8.8 8.7 8.9 13.8 12.7 14.7 15.0 15.5 16.2 13.0 13.0 13.4
Std Deviation (1e-4) 3.0 1.9 1.7 1.7 1.7 2.0 2.2 – 1.8 1.5 1.7 1.8 2.3 2.0 2.2 4.0 3.4 3.2 2.2 1.9 2.2
Intensity Change (Log2) – – – -0.02 -0.02 -0.62 -0.27 – -0.46 -0.51 -0.52 -0.49 0.15 0.03 0.24 0.26 0.31 0.38 0.06 0.06 0.1


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Cell Periphery 1.2 1.8 0 3.0 – 2.1 3.5 2.7 3.7 0 1.8 1.0 0 0 0
Cytoplasm 0 -4.8 -4.6 -6.6 – -7.2 -8.4 -9.0 -8.2 1.7 0.6 0.8 -1.4 -0.2 -0.1
Endoplasmic Reticulum 0 0 0 2.6 – 0 2.4 0 4.4 0 0 0 0 0 0
Endosome 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 5.9 0 – 6.7 6.7 7.2 6.1 0 0 0 0 0 0
Nucleus 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Vacuole 0 4.8 3.4 6.0 – 4.0 5.6 5.7 4.3 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 8.6143 11.7056 10.3824 9.9231 9.327 10.4563 11.5456 14.2962 13.2496 12.3068 10.5314 12.8308 15.3223 18.381 16.6513 16.159 16.0879 16.5626
Actin 0.0121 0.0002 0.0007 0.0001 0.0066 0.0001 0.0137 0.0001 0.0051 0.0003 0.0172 0.0079 0.0014 0.0002 0.001 0.0005 0.0001 0.0004
Bud 0.0004 0.0001 0.0001 0.0001 0.0002 0.0001 0.0036 0.0001 0.0003 0.0006 0.0005 0.0001 0.0001 0 0.0002 0.0002 0 0.0001
Bud Neck 0.0007 0.0001 0.0002 0.0002 0.0005 0.0004 0.0006 0.0001 0.0002 0.0002 0.0007 0.0005 0.0001 0.0001 0.0003 0.0003 0.0003 0.0007
Bud Periphery 0.0003 0 0 0 0.0003 0 0.0013 0 0.0001 0.0002 0.0007 0 0 0 0.0001 0 0 0.0001
Bud Site 0.0008 0.0002 0.0001 0 0.0002 0 0.0034 0.0001 0.0004 0.0001 0.0014 0 0.0001 0.0001 0.0013 0.0002 0 0
Cell Periphery 0.0002 0 0 0 0.0001 0 0.0004 0 0.0001 0 0.0002 0 0 0 0.0001 0 0 0
Cytoplasm 0.8781 0.9934 0.9888 0.9886 0.9162 0.9892 0.8809 0.9955 0.9656 0.9716 0.8079 0.97 0.9759 0.9948 0.9844 0.98 0.9927 0.9857
Cytoplasmic Foci 0.0129 0.0013 0.0014 0.0021 0.0091 0.0022 0.0104 0.0014 0.0068 0.0032 0.0279 0.0054 0.0032 0.0011 0.0025 0.0062 0.0015 0.0018
Eisosomes 0.0001 0 0 0 0.0001 0 0.0003 0 0 0 0.0002 0 0 0 0 0 0 0
Endoplasmic Reticulum 0.0046 0.0012 0.0038 0.0015 0.0042 0.0027 0.0037 0.0003 0.002 0.0128 0.0046 0.0017 0.0091 0.0009 0.0015 0.0035 0.0016 0.0028
Endosome 0.0213 0.0007 0.0014 0.0031 0.0195 0.0018 0.0257 0.0005 0.0076 0.0042 0.0508 0.0083 0.0035 0.0008 0.003 0.0037 0.0013 0.002
Golgi 0.0068 0.0001 0.0002 0.0001 0.0034 0.0001 0.0071 0.0001 0.0054 0.0001 0.0225 0.0015 0.0002 0.0002 0.0014 0.0005 0 0.0004
Lipid Particles 0.0054 0 0 0 0.0009 0 0.0047 0 0.0004 0 0.0134 0.0001 0.0001 0 0.0001 0 0 0
Mitochondria 0.0138 0.0001 0.0001 0.0001 0.0055 0.0001 0.0152 0 0.0021 0.0001 0.0227 0.0005 0.0001 0 0.0018 0.0001 0.0001 0.0031
None 0.009 0.0006 0.0007 0.0006 0.0013 0.0005 0.0032 0.0004 0.0007 0.0006 0.0025 0.0006 0.0021 0.0004 0.0004 0.0006 0.0003 0.0004
Nuclear Periphery 0.0155 0.0005 0.0005 0.0008 0.0213 0.0005 0.007 0.0002 0.0005 0.0023 0.0077 0.0005 0.0013 0.0002 0.0003 0.0012 0.0004 0.0004
Nucleolus 0.0005 0 0 0 0.0002 0 0.0012 0 0 0 0.0003 0 0 0 0 0 0 0
Nucleus 0.0082 0.0013 0.0012 0.0015 0.0052 0.0015 0.008 0.0009 0.0011 0.0018 0.0042 0.0017 0.0017 0.0008 0.0008 0.0014 0.001 0.0015
Peroxisomes 0.0036 0 0 0 0.0002 0 0.0019 0 0.0005 0 0.0072 0.0003 0.0001 0 0.0002 0.0001 0 0
Punctate Nuclear 0.0022 0.0001 0.0001 0.0001 0.001 0.0001 0.0031 0 0.0002 0.0001 0.0028 0.0001 0.0003 0 0 0.0003 0 0
Vacuole 0.0024 0.0002 0.0005 0.001 0.0015 0.0005 0.0029 0.0002 0.0008 0.0013 0.0026 0.0006 0.0007 0.0002 0.0004 0.0007 0.0003 0.0003
Vacuole Periphery 0.001 0.0001 0.0001 0.0003 0.0025 0.0001 0.0013 0 0.0002 0.0003 0.002 0.0002 0.0001 0 0.0001 0.0002 0.0001 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 60.6872 75.6222 69.5337 66.6657 92.9898 44.1117 61.0861 65.6298 72.3229 60.6179
Translational Efficiency 1.9876 1.8345 1.7642 1.947 1.5469 2.1247 1.8214 1.7339 1.3362 1.7715

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1367 1031 295 1621 2046 2157 191 1279 3413 3188 486 2900

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 1010.43 1363.43 1512.80 1608.13 1119.60 1348.21 1585.94 1722.60 1075.87 1353.13 1541.54 1658.62
Standard Deviation 897.49 226.41 190.97 263.15 159.64 203.15 216.85 293.00 583.75 211.07 204.68 282.49
Intensity Change Log 2 — 0.432271 0.582252 0.670415 — 0.268062 0.502355 0.621604 — 0.348292 0.540808 0.644965

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000514 0.000276 0.000591 0.000317 0.000194 0.000128 0.000629 0.003290 0.000322 0.000176 0.000606 0.001628
Bud Neck 0.001632 0.000440 0.003978 0.001104 0.000448 0.000538 0.002482 0.002710 0.000922 0.000506 0.003390 0.001812
Bud Site 0.039093 0.009014 0.017608 0.010960 0.006092 0.006724 0.012668 0.018863 0.019310 0.007464 0.015667 0.014446
Cell Periphery 0.000702 0.000942 0.000209 0.000186 0.000066 0.000048 0.000210 0.000221 0.000321 0.000337 0.000209 0.000201
Cytoplasm 0.685596* 0.907572* 0.713535* 0.890118* 0.849706* 0.903583* 0.769325* 0.852254* 0.783975* 0.904873* 0.735461* 0.873418*
Cytoplasmic Foci 0.170589 0.036315 0.060840 0.009305 0.094708 0.041972 0.044068 0.020454 0.125100 0.040143 0.054248 0.014222
Eisosomes 0.000017 0.000004 0.000011 0.000003 0.000006 0.000003 0.000009 0.000008 0.000010 0.000003 0.000010 0.000005
Endoplasmic Reticulum 0.006051 0.000973 0.001286 0.000119 0.001262 0.000612 0.000617 0.000264 0.003180 0.000729 0.001023 0.000183
Endosome 0.004448 0.001974 0.012416 0.001830 0.002914 0.002491 0.003271 0.005493 0.003528 0.002324 0.008822 0.003446
Golgi 0.011327 0.003943 0.001359 0.001554 0.005157 0.003384 0.001461 0.010284 0.007628 0.003565 0.001399 0.005404
Lipid Particles 0.001082 0.000874 0.003638 0.001489 0.000716 0.000521 0.002065 0.002418 0.000863 0.000635 0.003020 0.001899
Mitochondria 0.006014 0.000165 0.000085 0.000103 0.000774 0.000308 0.000070 0.000976 0.002873 0.000261 0.000079 0.000488
Mitotic Spindle 0.001360 0.002263 0.009140 0.004930 0.001037 0.002044 0.012091 0.016507 0.001166 0.002115 0.010300 0.010036
None 0.014068 0.003064 0.000642 0.002296 0.001965 0.002301 0.005309 0.001444 0.006812 0.002548 0.002476 0.001920
Nuclear Periphery 0.000059 0.000032 0.000355 0.000079 0.000028 0.000051 0.000279 0.000123 0.000040 0.000045 0.000325 0.000099
Nuclear Periphery Foci 0.001116 0.002658 0.008693 0.001362 0.001166 0.000716 0.007972 0.003184 0.001146 0.001344 0.008409 0.002166
Nucleolus 0.000354 0.000061 0.000129 0.000179 0.000378 0.000167 0.000129 0.000265 0.000368 0.000133 0.000129 0.000217
Nucleus 0.025483 0.012677 0.027079 0.035470 0.014211 0.013693 0.065465 0.025347 0.018726 0.013364 0.042165 0.031005
Peroxisomes 0.000659 0.000255 0.000442 0.000290 0.000315 0.000309 0.000226 0.001546 0.000453 0.000292 0.000357 0.000844
Vacuole 0.016013 0.013436 0.136826 0.036568 0.016570 0.018751 0.070985 0.030797 0.016347 0.017032 0.110950 0.034023
Vacuole Periphery 0.013822 0.003065 0.001138 0.001737 0.002288 0.001655 0.000669 0.003553 0.006908 0.002111 0.000954 0.002538

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 4.76 -1.47 3.30 -0.90 3.81 1.09 -4.18 -2.65 -2.75 -2.05 3.63 -4.80 -2.48 -2.93 -1.54
Bud Neck 6.26 -4.90 0.22 -9.52 5.15 -1.44 -7.93 -5.81 -5.46 1.78 4.32 -8.70 -5.89 -8.14 5.02
Bud Site 13.89 8.11 13.28 -0.33 4.06 -0.85 -4.59 -6.57 -5.91 -1.11 11.66 2.09 5.57 -4.96 2.47
Cell Periphery -0.25 4.46 4.86 0.96 1.58 4.13 -7.26 -9.66 -11.10 1.42 -0.00 2.02 2.95 0.55 2.02
Cytoplasm -26.08 1.66 -16.88 11.73 -10.70 -9.79 10.43 3.39 10.97 -8.61 -24.73 10.00 -7.83 16.41 -13.68
Cytoplasmic Foci 24.21 17.12 30.99 10.22 11.44 14.32 9.09 21.56 9.93 5.66 26.56 17.08 37.06 15.54 12.42
Eisosomes 7.44 3.72 12.72 0.17 9.19 3.30 -4.58 -0.58 -3.96 3.99 8.59 -1.89 6.79 -2.72 8.97
Endoplasmic Reticulum 7.42 7.75 9.75 2.63 5.32 2.75 3.44 5.80 2.67 3.73 8.02 7.73 11.21 3.88 6.20
Endosome 6.75 -5.52 7.41 1.42 7.94 1.02 -0.81 -0.49 -1.03 0.06 3.95 -5.83 3.35 0.24 7.00
Golgi 7.97 16.56 15.43 3.85 0.77 2.81 7.96 -0.30 -1.49 -3.18 7.66 17.07 6.16 0.67 -2.87
Lipid Particles 1.03 -7.51 -4.22 -4.47 3.84 1.96 -5.84 -7.20 -7.59 -0.02 2.42 -9.85 -8.44 -9.10 2.84
Mitochondria 6.89 7.01 7.00 1.42 -0.43 1.67 2.55 0.71 -1.23 -2.55 6.84 7.36 6.66 -0.27 -2.58
Mitotic Spindle -1.29 -3.76 -2.91 -1.69 2.77 -1.53 -2.77 -4.77 -4.19 0.26 -1.92 -4.69 -5.59 -4.44 2.07
None 4.68 6.21 5.59 1.07 -5.42 -0.79 -1.60 2.09 2.21 2.14 4.51 5.38 5.71 1.87 -0.05
Nuclear Periphery 3.18 -10.21 -5.71 -7.71 7.79 -1.02 -9.84 -5.85 -3.04 6.66 -0.34 -14.34 -8.73 -4.97 10.21
Nuclear Periphery Foci -5.68 -10.13 -4.38 2.59 9.02 3.35 -8.79 -4.36 -5.23 5.56 -2.15 -13.35 -5.88 -4.65 10.33
Nucleolus 4.74 3.50 2.58 -7.44 -2.90 0.73 0.76 0.62 -0.45 -0.83 1.31 1.27 0.99 -1.35 -2.05
Nucleus 5.78 -1.45 -8.18 -19.61 -6.05 0.25 -9.24 -13.39 -13.51 5.88 5.25 -8.98 -16.10 -23.63 1.72
Peroxisomes 5.23 3.52 4.81 0.05 2.08 0.10 1.13 -2.55 -2.60 -2.85 3.35 1.96 -0.89 -2.23 -1.67
Vacuole 1.09 -13.72 -18.55 -18.48 8.01 -2.83 -10.15 -14.23 -12.55 6.33 -1.99 -16.98 -23.35 -21.69 10.26
Vacuole Periphery 6.35 9.54 9.20 1.86 -0.19 1.49 5.10 0.53 -0.44 -2.51 6.89 10.49 8.21 1.35 -2.03
Standard name
Human Ortholog
Description Part of the heteromeric co-chaperone GimC/prefoldin complex; complex promotes efficient protein folding
Localization
Cell Percentages cytoplasm (99%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-7

Pac10

Pac10


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Pac10-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available