Standard name
Human Ortholog
Description Protein required for pre-rRNA processing; associated with the 90S pre-ribosome and 43S small ribosomal subunit precursor; interacts with U3 snoRNA; deletion mutant has synthetic fitness defect with an sgs1 deletion mutant
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 – 0 0 0 0 – 0 0 0.09 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 – 0 0 0.1 0.07 – 0.07 0.06 0.08 0.05 0.05 0.11 0.14 0.07 0.07 0.07 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 – 0 0.07 0 0.14 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05
Golgi 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 – 0 0 0.43 0 – 0.19 0.26 0.26 0.22 0 0 0 0.1 0.06 0 0 0 0 0 0 0
Nucleus 0.77 0.66 – 0.18 0.24 0.27 0.32 – 0.34 0.32 0.32 0.32 0.31 0.17 0.14 0.31 0.31 0.37 0.56 0.59 0.36 0.32 0.16 0.12
Nuclear Periphery 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.13 0.24 – 0.92 0.73 0.58 0.59 – 0.64 0.51 0.53 0.56 0.72 0.75 0.74 0.61 0.57 0.56 0.37 0.27 0.55 0.51 0.55 0.53
Peroxisomes 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 – 0.05 0.08 0.06 0.1 – 0 0.06 0 0.07 0 0.05 0.05 0 0 0 0 0 0 0 0.08 0.07
Vac/Vac Membrane 0.13 0.12 – 0 0 0 0 – 0 0 0 0 0 0 0 0.1 0.07 0.07 0 0 0 0.06 0.1 0.14
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0
Bud 0 1 – 0 0 0 0 – 0 1 2 1 0 1 0 0 0 0 3 3 0 3 7 5
Bud Neck 0 0 – 1 0 0 0 – 1 1 0 0 0 0 0 0 0 0 0 0 0 0 0 2
Bud Site 0 1 – 0 0 3 0 – 2 3 9 3 0 0 1 0 0 1 – – – – – –
Cell Periphery 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 5 5 – 1 3 9 9 – 5 8 8 6 10 46 67 8 6 10 1 1 0 2 1 1
Endoplasmic Reticulum 0 1 – 0 0 0 0 – 0 0 0 0 0 1 0 2 1 0 0 0 0 0 2 1
Endosome 1 1 – 3 7 0 19 – 0 1 1 0 7 10 21 0 3 2 1 3 0 4 10 14
Golgi 0 0 – 0 0 0 0 – 0 0 0 0 0 0 1 0 0 0 0 1 0 0 0 1
Mitochondria 0 3 – 2 1 38 4 – 14 33 25 28 1 3 4 12 5 4 0 2 0 1 3 3
Nucleus 111 118 – 16 25 24 43 – 25 40 31 42 65 73 67 37 28 57 93 120 8 62 51 34
Nuclear Periphery 0 0 – 0 0 0 2 – 0 1 0 3 0 0 0 0 0 0 0 0 0 1 3 4
Nucleolus 19 43 – 80 75 51 80 – 47 64 52 73 150 325 353 72 51 85 62 55 13 98 178 146
Peroxisomes 0 0 – 0 0 0 0 – 0 1 0 0 0 2 8 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 – 4 8 5 14 – 2 7 2 9 2 20 23 0 0 1 1 3 0 3 27 20
Vac/Vac Membrane 19 21 – 1 1 0 5 – 0 1 0 2 2 3 3 12 6 10 2 6 0 12 32 38
Unique Cell Count 145 180 87 103 88 136 74 125 98 130 207 433 476 119 89 153 167 205 24 193 322 278
Labelled Cell Count 155 194 108 120 130 176 96 161 130 167 237 484 548 143 100 170 167 205 24 193 322 278


Nucleolus, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 13.5 11.9 – 11.5 10.9 5.6 8.0 – 6.0 6.0 5.9 6.0 13.9 13.4 12.7 15.7 17.8 18.4 12.2 13.0 12.8
Std Deviation (1e-4) 2.5 3.0 – 2.2 3.0 1.8 1.7 – 1.6 1.4 1.4 1.6 3.5 4.5 4.5 5.1 5.5 5.2 2.5 2.6 2.5
Intensity Change (Log2) – – – – – – – – – – – – – – – – – – – – –


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 7.4097 8.5649 7.4363 6.3112 6.9928 7.412 9.2244 11.5836 10.7787 9.4948 9.4572 10.3478 11.2856 13.695 11.1639 10.8865 9.8677 11.2442
Actin 0.005 0 0.0001 0 0.0001 0.0004 0.0149 0 0.012 0.0029 0.0001 0.0016 0.0097 0 0 0 0.0001 0
Bud 0.0001 0 0 0 0.0001 0.0001 0.0005 0.0001 0.0002 0.0029 0.0016 0.0003 0.0001 0 0 0.0001 0.0001 0
Bud Neck 0.0003 0.0002 0.0001 0.0004 0.0008 0.0006 0.0012 0.0003 0.0021 0.0007 0.0002 0.0013 0.0004 0.0002 0.0001 0.0003 0.0003 0.0006
Bud Periphery 0.0001 0 0.0001 0.0001 0.0002 0.0002 0.001 0.0001 0.0003 0.0114 0.004 0.0007 0.0002 0 0 0.0001 0.0002 0.0001
Bud Site 0.0003 0.0001 0.0001 0.0001 0.0002 0.0001 0.0034 0.0002 0.0017 0.002 0.0015 0.0004 0.0004 0.0001 0.0001 0.0002 0.0003 0.0001
Cell Periphery 0.0002 0 0.0001 0.0001 0.0002 0.0002 0.0007 0.0002 0.0003 0.0012 0.0005 0.0005 0.0001 0.0001 0.0001 0.0001 0.0003 0.0001
Cytoplasm 0.0014 0 0.0005 0 0.0002 0.0001 0.0068 0.0001 0.0028 0.0006 0.0004 0.0011 0.0016 0 0.0001 0 0.0002 0
Cytoplasmic Foci 0.0103 0 0.0014 0.0001 0.0014 0.0005 0.0098 0.0001 0.0057 0.0199 0.0013 0.0029 0.0011 0 0.0005 0.0001 0.003 0
Eisosomes 0.0002 0 0 0 0 0 0.0002 0 0.0001 0.0001 0 0 0.0002 0 0 0 0.0001 0
Endoplasmic Reticulum 0.0005 0 0.0003 0 0.0001 0.0001 0.0029 0 0.0023 0.0026 0.0005 0.0018 0.0006 0 0.0001 0 0.0001 0
Endosome 0.0052 0 0.002 0 0.0005 0.001 0.0172 0 0.0087 0.0182 0.0243 0.0101 0.0017 0 0.0002 0.0001 0.0005 0
Golgi 0.0015 0 0.0002 0 0.0002 0.0002 0.0056 0 0.005 0.0061 0.0026 0.0033 0.001 0 0 0 0.0002 0
Lipid Particles 0.0142 0 0.0017 0.0002 0.0046 0.0033 0.0265 0.0001 0.0055 0.0089 0.0017 0.0042 0.0013 0 0.0014 0.0003 0.0088 0.0004
Mitochondria 0.0025 0.0001 0.0054 0.0001 0.0008 0.0022 0.0151 0.0003 0.0068 0.006 0.0166 0.0047 0.0007 0 0.0002 0.0002 0.0061 0.0001
None 0.0093 0 0.0005 0 0.0002 0.0001 0.0015 0 0.0035 0.0002 0.0002 0.0006 0.0017 0 0.0002 0 0.0003 0
Nuclear Periphery 0.0052 0.0003 0.0021 0.0013 0.0138 0.006 0.012 0.0005 0.0043 0.0034 0.0015 0.0327 0.0034 0 0.0006 0.0009 0.0023 0.0001
Nucleolus 0.6476 0.6549 0.7215 0.7606 0.5941 0.721 0.5546 0.6515 0.6725 0.6005 0.6375 0.5496 0.6431 0.7088 0.7668 0.6472 0.5456 0.7019
Nucleus 0.2817 0.3428 0.2585 0.2336 0.358 0.258 0.3004 0.3449 0.2565 0.2901 0.2661 0.3533 0.3116 0.2898 0.2248 0.3479 0.4207 0.2928
Peroxisomes 0.0026 0 0.0001 0 0.0001 0.0005 0.0084 0 0.0024 0.0024 0.0003 0.0009 0.0002 0 0.0001 0 0.0002 0
Punctate Nuclear 0.0099 0.0014 0.0028 0.0033 0.0221 0.0045 0.009 0.0007 0.0047 0.0014 0.0004 0.0247 0.0204 0.0006 0.004 0.0021 0.0082 0.0033
Vacuole 0.001 0.0001 0.0009 0.0002 0.0011 0.0005 0.0056 0.0006 0.0014 0.0122 0.0238 0.0032 0.0004 0.0002 0.0003 0.0003 0.001 0.0002
Vacuole Periphery 0.001 0 0.0014 0.0001 0.0014 0.0004 0.0026 0.0003 0.0011 0.0061 0.0148 0.0021 0.0003 0.0001 0.0002 0.0001 0.0013 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 38.349 23.3738 18.5007 29.2069 29.3807 1.7422 22.0577 22.8629 18.8728 31.9724
Translational Efficiency – – – – – – – – – –

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency –

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
206 1233 199 1748 2334 1980 1976 1495 2540 3213 2175 3243

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 928.83 1247.64 1464.84 1207.10 1104.41 1283.07 1436.91 1479.98 1090.17 1269.47 1439.47 1332.90
Standard Deviation 150.21 271.75 204.21 246.85 181.43 218.71 261.27 320.63 185.41 241.07 256.71 314.23
Intensity Change Log 2 — 0.425715 0.657257 0.378059 — 0.216324 0.379694 0.422302 — 0.315762 0.513143 0.402259

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000570 0.000461 0.000999 0.000859 0.000247 0.000318 0.000528 0.000671 0.000273 0.000373 0.000571 0.000772
Bud Neck 0.006051 0.005737 0.014070 0.022279 0.004516 0.007337 0.011908 0.023799 0.004641 0.006723 0.012106 0.022980
Bud Site 0.001268 0.000641 0.002166 0.004762 0.000445 0.000742 0.000552 0.001672 0.000512 0.000704 0.000700 0.003337
Cell Periphery 0.000117 0.000056 0.000058 0.000336 0.000040 0.000061 0.000028 0.000088 0.000047 0.000059 0.000030 0.000222
Cytoplasm 0.004832 0.000548 0.000296 0.012868 0.000192 0.000150 0.000122 0.001248 0.000568 0.000303 0.000138 0.007511
Cytoplasmic Foci 0.004521 0.000634 0.000420 0.001251 0.000276 0.000196 0.000050 0.000451 0.000621 0.000364 0.000084 0.000882
Eisosomes 0.000054 0.000065 0.000062 0.000044 0.000055 0.000093 0.000052 0.000054 0.000055 0.000082 0.000053 0.000048
Endoplasmic Reticulum 0.003514 0.001008 0.001482 0.002721 0.000957 0.001194 0.000562 0.000730 0.001164 0.001123 0.000647 0.001803
Endosome 0.003047 0.001029 0.000176 0.001337 0.000327 0.000868 0.000051 0.000527 0.000548 0.000930 0.000062 0.000963
Golgi 0.000501 0.000662 0.000345 0.000498 0.000238 0.000220 0.000100 0.000427 0.000259 0.000389 0.000122 0.000465
Lipid Particles 0.002296 0.000249 0.000123 0.000266 0.000065 0.000061 0.000012 0.000071 0.000246 0.000133 0.000022 0.000176
Mitochondria 0.000974 0.001117 0.001967 0.002632 0.000619 0.001398 0.001315 0.002881 0.000648 0.001290 0.001375 0.002746
Mitotic Spindle 0.001945 0.001075 0.001758 0.007143 0.001851 0.001952 0.000294 0.005873 0.001859 0.001616 0.000428 0.006557
None 0.001584 0.001753 0.001034 0.017326 0.000565 0.000592 0.000565 0.001424 0.000648 0.001038 0.000608 0.009996
Nuclear Periphery 0.000879 0.000251 0.000425 0.000966 0.000252 0.000297 0.000469 0.002686 0.000303 0.000280 0.000465 0.001759
Nuclear Periphery Foci 0.006806 0.000711 0.000468 0.001746 0.000644 0.000445 0.000104 0.001466 0.001144 0.000547 0.000137 0.001617
Nucleolus 0.464221* 0.432893* 0.548289* 0.216305 0.458886* 0.382787* 0.407692* 0.445848* 0.459319* 0.402016* 0.420556* 0.322123*
Nucleus 0.487721* 0.545214* 0.412067* 0.700942* 0.526672* 0.595880* 0.572646* 0.500257* 0.523513* 0.576437* 0.557954* 0.608428*
Peroxisomes 0.002472 0.002755 0.011129 0.001498 0.001522 0.001280 0.001632 0.002553 0.001599 0.001846 0.002501 0.001984
Vacuole 0.005552 0.001437 0.001941 0.003110 0.000972 0.001709 0.000446 0.003163 0.001344 0.001605 0.000583 0.003134
Vacuole Periphery 0.001077 0.001703 0.000724 0.001112 0.000658 0.002418 0.000870 0.004112 0.000692 0.002143 0.000857 0.002495

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 1.11 -3.89 -2.51 -10.11 2.85 -3.52 -14.50 -15.22 -13.10 -5.51 -4.09 -13.33 -21.01 -17.66 -7.47
Bud Neck 0.91 -5.66 -13.00 -17.13 -3.32 -5.33 -13.09 -18.14 -15.62 -10.77 -4.85 -14.24 -25.11 -22.79 -13.51
Bud Site 1.95 -2.50 -4.35 -6.16 -2.23 -2.37 -2.67 -8.42 -6.56 -7.63 -1.64 -3.78 -7.90 -7.50 -7.06
Cell Periphery 1.96 1.40 -3.23 -5.56 -4.94 -1.50 1.37 -4.99 -2.67 -8.30 -1.07 1.72 -6.35 -5.95 -7.26
Cytoplasm 2.44 2.56 -3.42 -8.09 -8.30 0.74 0.85 -4.58 -4.75 -4.77 1.55 2.65 -8.47 -8.89 -9.10
Cytoplasmic Foci 2.01 2.10 1.77 -1.00 -1.61 0.74 2.14 -1.62 -2.79 -4.71 1.31 2.82 -0.58 -1.92 -3.37
Eisosomes -1.75 -2.25 2.12 5.91 5.68 -8.55 1.35 0.60 7.12 -0.14 -8.24 0.71 2.79 9.99 2.39
Endoplasmic Reticulum 3.79 2.34 1.16 -4.41 -1.75 -1.99 5.15 1.96 3.75 -3.61 0.51 5.13 -2.93 -3.27 -5.38
Endosome 1.45 2.08 1.37 -0.38 -4.15 -1.63 3.70 -1.69 0.92 -4.63 -1.26 3.67 -1.81 0.07 -6.20
Golgi -0.42 0.67 0.20 0.54 -0.46 0.20 1.49 -1.97 -2.84 -4.99 -0.87 1.52 -1.97 -0.60 -4.76
Lipid Particles 1.38 1.43 1.38 -0.12 -1.01 0.19 1.58 -0.36 -0.84 -4.57 0.87 1.77 0.54 -0.73 -3.94
Mitochondria -0.43 -3.64 -5.06 -4.87 -0.77 -1.79 -7.10 -12.89 -3.72 -9.53 -2.24 -7.90 -12.26 -5.02 -8.14
Mitotic Spindle 1.18 0.44 -3.63 -5.16 -4.81 -0.11 2.04 -3.62 -3.65 -5.03 0.30 2.05 -5.43 -6.16 -7.88
None -0.18 0.98 -7.89 -7.37 -8.13 -0.19 -0.23 -6.28 -5.69 -6.05 -1.38 0.15 -8.95 -8.21 -8.96
Nuclear Periphery 3.07 1.85 -0.05 -5.65 -2.93 -0.63 -1.65 -4.75 -4.66 -4.20 0.53 -1.34 -5.85 -6.04 -4.77
Nuclear Periphery Foci 3.78 3.97 3.17 -1.62 -2.27 1.00 4.17 -2.31 -2.80 -4.16 2.56 5.61 -1.13 -3.01 -4.79
Nucleolus 1.45 -1.33 13.45 22.28* 13.15 9.34 6.09 2.57 -4.99 -2.70 7.96 5.40 19.39 12.57 11.84
Nucleus -3.06 1.32 -11.53 -15.07 -11.58 -8.54 -5.37 2.43 9.33 6.74 -7.45 -4.67 -11.44 -4.72 -5.59
Peroxisomes -0.60 -5.51 2.04 3.99 6.23 2.09 -1.38 -5.32 -6.73 -4.43 -1.88 -4.99 -2.65 -1.14 2.41
Vacuole 1.41 0.92 0.37 -3.60 -1.78 -1.23 1.34 -3.22 -2.52 -4.85 -0.32 1.57 -3.88 -4.40 -6.78
Vacuole Periphery -1.00 0.65 0.10 1.41 -1.35 -3.78 -1.31 -7.95 -2.66 -7.94 -4.09 -1.11 -6.85 -0.65 -7.45
Standard name
Human Ortholog
Description Protein required for pre-rRNA processing; associated with the 90S pre-ribosome and 43S small ribosomal subunit precursor; interacts with U3 snoRNA; deletion mutant has synthetic fitness defect with an sgs1 deletion mutant
Localization
Cell Percentages nucleolus (44%), nucleus (18%), mixed (37%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Slx9

Slx9


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Slx9-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available