Standard name | |
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Human Ortholog | |
Description | Ser/Thr kinase involved in transcription and stress response; functions as part of a network of genes in exit from mitosis; localization is cell cycle regulated; activated by Cdc15p during the exit from mitosis; also plays a role in regulating the stability of SWI5 and CLB2 mRNAs; phosphorylates Chs2p to regulate primary septum formation and Hof1p to regulate cytokinesis; DBF2 has a paralog, DBF20, that arose from the whole genome duplication |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 18 | 36 | 19 | 26 | 24 | 27 | 28 | – | 8 | 9 | 3 | 3 | 0 | 0 | 0 | 12 | 10 | 8 | 9 | 16 | 11 | 2 | 0 | 4 |
Bud | 2 | 4 | 3 | 6 | 6 | 3 | 13 | – | 7 | 9 | 8 | 6 | 0 | 0 | 0 | 1 | 1 | 1 | 11 | 16 | 5 | 2 | 1 | 3 |
Bud Neck | 0 | 0 | 0 | 3 | 3 | 1 | 5 | – | 5 | 4 | 1 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 19 | 26 | 10 | 4 | 0 | 1 |
Bud Site | 1 | 1 | 0 | 3 | 4 | 12 | 9 | – | 2 | 0 | 3 | 1 | 0 | 0 | 0 | 3 | 1 | 0 | – | – | – | – | – | – |
Cell Periphery | 3 | 15 | 13 | 9 | 8 | 4 | 7 | – | 4 | 2 | 2 | 4 | 5 | 4 | 12 | 26 | 20 | 15 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 264 | 276 | 277 | 227 | 273 | 213 | 309 | – | 215 | 245 | 165 | 214 | 173 | 258 | 249 | 173 | 158 | 162 | 238 | 257 | 256 | 71 | 160 | 167 |
Endoplasmic Reticulum | 11 | 10 | 6 | 9 | 4 | 11 | 15 | – | 1 | 2 | 6 | 6 | 0 | 3 | 1 | 8 | 4 | 10 | 3 | 1 | 4 | 0 | 0 | 1 |
Endosome | 5 | 6 | 4 | 4 | 5 | 5 | 0 | – | 0 | 3 | 2 | 0 | 2 | 1 | 1 | 21 | 24 | 20 | 8 | 12 | 9 | 3 | 4 | 10 |
Golgi | 1 | 3 | 1 | 0 | 0 | 0 | 0 | – | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 5 | 5 | 1 | 2 | 3 | 1 | 0 | 0 | 2 |
Mitochondria | 2 | 2 | 1 | 0 | 0 | 8 | 18 | – | 31 | 24 | 80 | 31 | 0 | 0 | 0 | 4 | 2 | 4 | 1 | 2 | 4 | 0 | 3 | 6 |
Nucleus | 1 | 0 | 1 | 0 | 1 | 3 | 7 | – | 1 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 1 | 0 | 1 | 1 | 3 | 2 | 2 | – | 1 | 0 | 1 | 1 | 0 | 0 | 0 | 18 | 11 | 12 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 1 | 3 | 0 | 1 | 3 | 1 | 3 | – | 3 | 2 | 0 | 0 | 0 | 0 | 0 | 1 | 2 | 0 | 0 | 1 | 0 | 0 | 0 | 3 |
SpindlePole | 1 | 0 | 6 | 9 | 10 | 3 | 17 | – | 6 | 1 | 4 | 2 | 0 | 1 | 0 | 32 | 21 | 17 | 3 | 5 | 6 | 0 | 0 | 2 |
Vac/Vac Membrane | 0 | 0 | 1 | 2 | 2 | 2 | 2 | – | 2 | 2 | 4 | 3 | 2 | 2 | 0 | 22 | 13 | 11 | 2 | 3 | 16 | 1 | 2 | 5 |
Unique Cell Count | 298 | 328 | 306 | 272 | 319 | 267 | 376 | 256 | 278 | 229 | 251 | 177 | 264 | 251 | 255 | 211 | 214 | 304 | 351 | 330 | 90 | 178 | 214 | |
Labelled Cell Count | 311 | 356 | 333 | 300 | 346 | 295 | 435 | 286 | 304 | 281 | 274 | 182 | 269 | 263 | 327 | 272 | 261 | 304 | 351 | 330 | 90 | 178 | 214 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 7.7 | 9.9 | 8.7 | 8.1 | 8.0 | 6.8 | 6.4 | – | 5.3 | 5.1 | 4.4 | 4.6 | 8.3 | 8.3 | 7.5 | 12.0 | 12.5 | 11.2 | 7.4 | 8.2 | 8.7 |
Std Deviation (1e-4) | 1.1 | 1.5 | 1.1 | 1.3 | 1.6 | 1.5 | 1.2 | – | 1.1 | 1.1 | 0.7 | 0.9 | 1.4 | 1.2 | 1.3 | 2.2 | 2.5 | 2.1 | 1.1 | 1.4 | 1.3 |
Intensity Change (Log2) | – | – | – | -0.11 | -0.12 | -0.35 | -0.43 | – | -0.72 | -0.77 | -0.98 | -0.93 | -0.07 | -0.08 | -0.22 | 0.46 | 0.52 | 0.37 | -0.22 | -0.09 | 0.01 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 1.5 | 0.6 | 1.7 | 0.6 | – | -1.7 | -1.7 | 0 | 0 | -3.4 | -4.1 | -4.0 | -0.8 | -0.7 | -1.2 |
Bud | 0 | 0 | 0 | 2.1 | – | 0 | 1.9 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 3.7 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | -0.6 | -1.2 | -1.9 | -1.8 | – | -1.9 | -2.7 | 0 | 0 | -0.8 | -1.9 | 0.3 | 2.8 | 2.4 | 1.4 |
Cytoplasm | -2.5 | -1.9 | -3.6 | -3.1 | – | -2.3 | -0.9 | -5.6 | -1.9 | 3.0 | 3.6 | 4.4 | -6.7 | -4.8 | -4.6 |
Endoplasmic Reticulum | 1.0 | 0 | 1.5 | 1.5 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0.9 | 0 | 1.8 |
Endosome | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 4.0 | 5.0 | 4.3 |
Golgi | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | 3.5 | – | 6.0 | 5.0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 4.4 | 0 | 3.8 |
Peroxisomes | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 1.0 | 0.9 | 0 | 1.8 | – | 0.3 | 0 | 0 | 0 | 0 | 0 | 0 | 5.0 | 4.0 | 3.3 |
Vacuole | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 4.9 | 4.0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 4.1656 | 4.8838 | 3.7823 | 3.7877 | 3.7594 | 4.9031 | 3.7934 | 4.1051 | 3.1573 | 2.8662 | 3.4102 | 4.5224 | 3.9275 | 4.7572 | 3.7547 | 3.644 | 3.8714 | 4.8237 |
Actin | 0.0179 | 0.0063 | 0.0164 | 0.0048 | 0.0018 | 0.0069 | 0.0362 | 0.003 | 0.0089 | 0.1107 | 0.0054 | 0.0205 | 0.0865 | 0.0103 | 0.0226 | 0.0757 | 0.001 | 0.0406 |
Bud | 0.0017 | 0.0017 | 0.003 | 0.0035 | 0.0029 | 0.0011 | 0.0016 | 0.0012 | 0.0009 | 0.0016 | 0.0019 | 0.0003 | 0.0087 | 0.0069 | 0.0036 | 0.0008 | 0.0001 | 0.0008 |
Bud Neck | 0.0291 | 0.0017 | 0.0023 | 0.0007 | 0.0013 | 0.3533 | 0.034 | 0.0012 | 0.0019 | 0.0016 | 0.0045 | 0.459 | 0.0121 | 0.001 | 0.0151 | 0.0019 | 0.0795 | 0.421 |
Bud Periphery | 0.002 | 0.001 | 0.003 | 0.0035 | 0.0024 | 0.0009 | 0.0026 | 0.0009 | 0.001 | 0.0019 | 0.0019 | 0.0005 | 0.0069 | 0.0021 | 0.0035 | 0.0008 | 0.0001 | 0.0007 |
Bud Site | 0.0289 | 0.0565 | 0.0182 | 0.0027 | 0.0274 | 0.002 | 0.0514 | 0.0951 | 0.0123 | 0.0074 | 0.0184 | 0.0014 | 0.05 | 0.0717 | 0.0236 | 0.0009 | 0.0004 | 0.0021 |
Cell Periphery | 0.0011 | 0.0006 | 0.0005 | 0.0002 | 0.0002 | 0.0002 | 0.0035 | 0.0021 | 0.0009 | 0.0003 | 0.0004 | 0.0008 | 0.0013 | 0.0007 | 0.0006 | 0.0001 | 0.0001 | 0.0005 |
Cytoplasm | 0.6849 | 0.8631 | 0.7757 | 0.7832 | 0.6756 | 0.5276 | 0.6737 | 0.7833 | 0.8863 | 0.6035 | 0.7811 | 0.4126 | 0.6404 | 0.841 | 0.844 | 0.8072 | 0.8004 | 0.4538 |
Cytoplasmic Foci | 0.0604 | 0.0243 | 0.0389 | 0.041 | 0.0878 | 0.0424 | 0.0301 | 0.0157 | 0.0194 | 0.041 | 0.0628 | 0.0719 | 0.0735 | 0.0316 | 0.0094 | 0.0553 | 0.0469 | 0.0481 |
Eisosomes | 0.0001 | 0 | 0.0001 | 0 | 0 | 0 | 0.0005 | 0.0001 | 0 | 0.0006 | 0 | 0.0001 | 0.0005 | 0 | 0.0001 | 0.0002 | 0 | 0.0001 |
Endoplasmic Reticulum | 0.004 | 0.0025 | 0.0029 | 0.0035 | 0.0023 | 0.0016 | 0.0217 | 0.0027 | 0.0064 | 0.0026 | 0.0067 | 0.0032 | 0.0108 | 0.0036 | 0.0155 | 0.0026 | 0.0035 | 0.0024 |
Endosome | 0.0462 | 0.0074 | 0.0258 | 0.0476 | 0.087 | 0.0117 | 0.0261 | 0.0026 | 0.0135 | 0.0432 | 0.0418 | 0.0112 | 0.0234 | 0.0065 | 0.0157 | 0.0092 | 0.0269 | 0.005 |
Golgi | 0.0179 | 0.0018 | 0.0139 | 0.0198 | 0.0216 | 0.0028 | 0.0084 | 0.0007 | 0.0047 | 0.0373 | 0.0078 | 0.003 | 0.0146 | 0.0011 | 0.0043 | 0.0039 | 0.0166 | 0.0083 |
Lipid Particles | 0.0169 | 0.0002 | 0.0041 | 0.0082 | 0.0092 | 0.0015 | 0.0149 | 0.0003 | 0.0041 | 0.038 | 0.0143 | 0.0008 | 0.0145 | 0.0009 | 0.0035 | 0.0024 | 0.0098 | 0.0007 |
Mitochondria | 0.0125 | 0.0005 | 0.0112 | 0.0195 | 0.039 | 0.0081 | 0.0066 | 0.0001 | 0.0008 | 0.0312 | 0.0029 | 0.0002 | 0.0119 | 0.0004 | 0.0052 | 0.0006 | 0.001 | 0.001 |
None | 0.0377 | 0.023 | 0.0615 | 0.0325 | 0.0113 | 0.024 | 0.0472 | 0.0866 | 0.0271 | 0.0183 | 0.0213 | 0.0053 | 0.0125 | 0.0139 | 0.0172 | 0.0045 | 0.0024 | 0.0025 |
Nuclear Periphery | 0.0037 | 0.0009 | 0.001 | 0.0064 | 0.0012 | 0.0003 | 0.0102 | 0.0004 | 0.0009 | 0.0018 | 0.0024 | 0.0003 | 0.0019 | 0.0006 | 0.0036 | 0.0005 | 0.0005 | 0.0005 |
Nucleolus | 0.0007 | 0.0002 | 0.0003 | 0.0005 | 0.0007 | 0.0001 | 0.0006 | 0.0002 | 0.0002 | 0.0003 | 0.001 | 0.0001 | 0.0003 | 0.0001 | 0.0002 | 0.0002 | 0 | 0 |
Nucleus | 0.0027 | 0.0018 | 0.0018 | 0.0033 | 0.0022 | 0.0008 | 0.0044 | 0.0009 | 0.0009 | 0.0007 | 0.0037 | 0.0006 | 0.0008 | 0.001 | 0.0011 | 0.0065 | 0.0006 | 0.0012 |
Peroxisomes | 0.0173 | 0.0015 | 0.0145 | 0.0072 | 0.0041 | 0.0079 | 0.0174 | 0.0003 | 0.0047 | 0.0511 | 0.0124 | 0.0031 | 0.0201 | 0.0008 | 0.0077 | 0.0198 | 0.0082 | 0.0075 |
Punctate Nuclear | 0.0048 | 0.0031 | 0.0016 | 0.0054 | 0.0041 | 0.0055 | 0.0042 | 0.0006 | 0.0011 | 0.0038 | 0.0025 | 0.0038 | 0.0074 | 0.0039 | 0.0008 | 0.0011 | 0.001 | 0.0028 |
Vacuole | 0.0067 | 0.0017 | 0.0029 | 0.005 | 0.0124 | 0.0011 | 0.0036 | 0.002 | 0.0036 | 0.002 | 0.0059 | 0.0012 | 0.0013 | 0.0021 | 0.0023 | 0.0053 | 0.0008 | 0.0003 |
Vacuole Periphery | 0.0029 | 0.0002 | 0.0006 | 0.0015 | 0.0055 | 0.0002 | 0.0011 | 0.0001 | 0.0003 | 0.0009 | 0.0009 | 0.0001 | 0.0006 | 0.0002 | 0.0004 | 0.0005 | 0.0001 | 0.0001 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 9.6283 | 21.2309 | 35.3406 | 36.8498 | 15.8125 | 12.1891 | 26.9782 | 40.7243 | 39.4495 | 18.974 |
Translational Efficiency | 0.5526 | 0.8119 | 1.3445 | 1.0235 | 0.7474 | 0.8178 | 1.0752 | 1.0435 | 1.1261 | 1.0448 |
Dataset | Hit |
---|---|
Protein Concentration | ✔ |
Protein Localization | ✔ |
Gene Expression | ✔ |
Translational Efficiency | ✔ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
119 | 902 | 829 | 357 | 1885 | 1847 | 119 | 238 | 2004 | 2749 | 948 | 595 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 713.23 | 966.10 | 1234.59 | 1336.62 | 841.09 | 915.55 | 1324.69 | 1285.43 | 833.50 | 932.14 | 1245.90 | 1316.14 |
Standard Deviation | 81.46 | 141.11 | 139.78 | 253.54 | 106.96 | 125.38 | 140.48 | 213.98 | 109.85 | 132.89 | 143.01 | 239.82 |
Intensity Change Log 2 | — | 0.437805 | 0.791593 | 0.906150 | — | 0.122378 | 0.655323 | 0.611919 | — | 0.275715 | 0.719454 | 0.754412 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000153 | 0.001036 | 0.001834 | 0.012160 | 0.000710 | 0.002844 | 0.002679 | 0.003482 | 0.000677 | 0.002251 | 0.001940 | 0.008689 |
Bud Neck | 0.048746 | 0.017090 | 0.019181 | 0.012883 | 0.062761 | 0.045249 | 0.017954 | 0.015155 | 0.061929 | 0.036009 | 0.019027 | 0.013792 |
Bud Site | 0.034879 | 0.089492 | 0.196367* | 0.181206* | 0.089460 | 0.104955* | 0.169112* | 0.189240* | 0.086219 | 0.099882 | 0.192946* | 0.184420* |
Cell Periphery | 0.000470 | 0.000199 | 0.000263 | 0.000678 | 0.000266 | 0.000175 | 0.000424 | 0.000240 | 0.000279 | 0.000183 | 0.000283 | 0.000503 |
Cytoplasm | 0.456349* | 0.553351* | 0.625987* | 0.461558* | 0.500982* | 0.501101* | 0.412142* | 0.259574* | 0.498332* | 0.518245* | 0.599143* | 0.380764* |
Cytoplasmic Foci | 0.280788* | 0.204059* | 0.061525 | 0.158045 | 0.209342* | 0.180152* | 0.106403 | 0.205897* | 0.213584* | 0.187997* | 0.067158 | 0.177185 |
Eisosomes | 0.000343 | 0.000067 | 0.000016 | 0.000058 | 0.000177 | 0.000087 | 0.000061 | 0.000042 | 0.000187 | 0.000081 | 0.000022 | 0.000052 |
Endoplasmic Reticulum | 0.002952 | 0.000701 | 0.000638 | 0.000651 | 0.002029 | 0.000995 | 0.002845 | 0.000588 | 0.002084 | 0.000898 | 0.000915 | 0.000626 |
Endosome | 0.040364 | 0.024972 | 0.005286 | 0.013496 | 0.020391 | 0.030614 | 0.015110 | 0.041061 | 0.021577 | 0.028763 | 0.006519 | 0.024522 |
Golgi | 0.006434 | 0.005787 | 0.001187 | 0.015684 | 0.006284 | 0.011238 | 0.009917 | 0.039587 | 0.006293 | 0.009450 | 0.002283 | 0.025245 |
Lipid Particles | 0.011864 | 0.002120 | 0.001845 | 0.012561 | 0.003115 | 0.002613 | 0.004593 | 0.005364 | 0.003635 | 0.002451 | 0.002190 | 0.009682 |
Mitochondria | 0.002913 | 0.001117 | 0.000111 | 0.000166 | 0.001637 | 0.002838 | 0.000064 | 0.007595 | 0.001713 | 0.002273 | 0.000105 | 0.003138 |
Mitotic Spindle | 0.000156 | 0.008841 | 0.008520 | 0.035751 | 0.002084 | 0.005788 | 0.029788 | 0.083935 | 0.001969 | 0.006790 | 0.011190 | 0.055025 |
None | 0.000809 | 0.000534 | 0.001356 | 0.000227 | 0.000953 | 0.000735 | 0.000129 | 0.000188 | 0.000944 | 0.000669 | 0.001202 | 0.000212 |
Nuclear Periphery | 0.000291 | 0.000047 | 0.000092 | 0.000148 | 0.000075 | 0.000042 | 0.000222 | 0.000682 | 0.000088 | 0.000044 | 0.000108 | 0.000362 |
Nuclear Periphery Foci | 0.000823 | 0.001339 | 0.006680 | 0.002471 | 0.000874 | 0.000472 | 0.020707 | 0.003790 | 0.000871 | 0.000756 | 0.008441 | 0.002999 |
Nucleolus | 0.000470 | 0.000192 | 0.000078 | 0.000114 | 0.000323 | 0.000451 | 0.000057 | 0.000421 | 0.000332 | 0.000366 | 0.000076 | 0.000237 |
Nucleus | 0.024934 | 0.006624 | 0.023088 | 0.002922 | 0.015675 | 0.006329 | 0.011464 | 0.002642 | 0.016225 | 0.006426 | 0.021629 | 0.002810 |
Peroxisomes | 0.005968 | 0.007075 | 0.003234 | 0.034075 | 0.005966 | 0.012916 | 0.001880 | 0.040432 | 0.005967 | 0.010999 | 0.003064 | 0.036617 |
Vacuole | 0.076603 | 0.073918 | 0.042311 | 0.053434 | 0.075443 | 0.087969 | 0.193987 | 0.095105 | 0.075512 | 0.083358 | 0.061351 | 0.070103 |
Vacuole Periphery | 0.003691 | 0.001438 | 0.000401 | 0.001712 | 0.001451 | 0.002438 | 0.000459 | 0.004981 | 0.001584 | 0.002110 | 0.000408 | 0.003020 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -5.35 | -4.80 | -5.37 | -4.78 | -4.15 | -2.99 | -3.95 | -3.08 | 0.42 | -0.40 | -3.27 | -3.88 | -5.57 | -3.65 | -4.07 |
Bud Neck | 2.77 | 2.88 | 3.39 | 2.74 | 3.04 | 3.59 | 9.78 | 12.84 | 10.63 | 0.32 | 6.04 | 11.71 | 13.43 | 10.88 | 3.31 |
Bud Site | -5.07 | -9.60 | -7.49 | -3.84 | 2.03 | -2.34 | -0.89 | -2.18 | -1.12 | -0.48 | -2.29 | -6.81 | -4.60 | -3.26 | 2.23 |
Cell Periphery | 1.46 | 1.09 | -0.95 | -4.82 | -4.15 | 4.85 | -2.76 | 2.49 | -1.01 | 4.24 | 4.48 | -0.16 | -3.27 | -5.07 | -3.25 |
Cytoplasm | -3.64 | -6.25 | -0.19 | 4.45 | 7.72 | 0.01 | 3.90 | 8.84 | 8.76 | 3.02 | -2.12 | -7.65 | 6.48 | 7.85 | 11.19 |
Cytoplasmic Foci | 3.59 | 10.37 | 6.19 | 5.13 | -6.57 | 4.11 | 11.13 | 2.63 | 0.74 | -4.39 | 3.98 | 22.48 | 6.25 | 3.87 | -8.71 |
Eisosomes | 3.62 | 4.31 | 3.91 | 1.40 | -4.07 | 7.30 | 10.17 | 12.97 | 7.19 | 4.66 | 8.63 | 14.82 | 12.41 | 5.25 | -3.77 |
Endoplasmic Reticulum | 1.88 | 1.85 | 2.12 | 1.49 | 1.76 | 1.76 | -0.05 | 3.07 | 2.51 | 2.56 | 2.20 | 2.23 | 3.24 | 2.60 | 3.35 |
Endosome | 2.63 | 5.90 | 5.14 | 5.60 | -1.54 | -5.34 | 2.99 | -0.35 | 2.22 | -2.05 | -4.19 | 12.09 | 3.53 | 6.48 | -3.75 |
Golgi | 0.35 | 2.56 | -0.31 | -0.59 | -2.03 | -4.48 | 0.33 | -1.77 | -0.97 | -1.65 | -3.76 | 7.42 | -1.63 | -0.65 | -3.11 |
Lipid Particles | 2.12 | 2.02 | -0.82 | -5.13 | -4.97 | 0.76 | -2.02 | -1.95 | -2.10 | -1.01 | 2.24 | 1.75 | -4.78 | -5.37 | -5.23 |
Mitochondria | 1.29 | 2.15 | 2.14 | 1.90 | -0.33 | -1.39 | 3.01 | -0.77 | -0.22 | -1.54 | -0.78 | 3.27 | 0.33 | 0.89 | -1.53 |
Mitotic Spindle | -4.19 | -5.50 | -5.55 | -4.22 | -4.02 | -2.87 | -2.04 | -6.03 | -5.80 | -3.44 | -4.03 | -4.61 | -7.96 | -7.32 | -6.24 |
None | 1.11 | -0.88 | 2.48 | 1.17 | 3.34 | 0.92 | 6.55 | 6.02 | 2.59 | -0.73 | 1.44 | -0.59 | 5.95 | 2.67 | 3.44 |
Nuclear Periphery | 1.29 | 0.80 | 0.19 | -4.49 | -2.46 | 1.43 | -4.12 | -2.18 | -2.36 | -0.59 | 1.75 | -3.17 | -3.13 | -3.79 | -2.19 |
Nuclear Periphery Foci | -1.79 | -9.86 | -6.97 | -5.27 | 4.45 | 1.79 | -5.46 | -2.94 | -3.29 | 4.37 | 0.07 | -11.15 | -6.28 | -6.48 | 5.74 |
Nucleolus | 2.76 | 3.72 | 2.96 | 0.72 | -0.97 | -0.70 | 4.87 | -0.10 | 0.45 | -1.33 | -0.31 | 5.77 | 1.15 | 0.94 | -1.57 |
Nucleus | 5.21 | -0.93 | 5.55 | 1.40 | 12.45 | 11.28 | 0.40 | 12.18 | 2.10 | 4.33 | 12.95 | -6.38 | 13.97 | 2.04 | 13.49 |
Peroxisomes | -0.46 | 1.98 | -3.56 | -3.56 | -4.79 | -5.15 | 6.28 | -3.89 | -2.31 | -4.89 | -4.82 | 5.10 | -5.44 | -3.65 | -6.67 |
Vacuole | 0.11 | 1.02 | -3.97 | -5.42 | -6.26 | -3.28 | -9.42 | -7.24 | -6.46 | 2.74 | -2.62 | -3.14 | -8.97 | -7.99 | -6.37 |
Vacuole Periphery | 1.17 | 1.72 | 1.43 | 1.36 | -1.42 | -3.15 | 6.48 | -0.69 | 0.20 | -1.75 | -1.93 | 6.29 | 0.35 | 1.33 | -2.18 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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