Standard name
Human Ortholog
Description Mitochondrial and cytoplasmic valyl-tRNA synthetase; human homolog VARS2 implicated in mitochondrial diseases, can partially complement yeast null mutant
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 – 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0.06 0.07 0.06 0.05 – 0.13 0.13 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 1.0 0.98 0.99 0.99 0.95 0.92 0.82 0.79 0.78 – 0.77 0.71 0.99 0.99 0.98 0.97 0.98 0.99 0.97 0.96 0.95 0.94 0.94 0.95
Endoplasmic Reticulum 0 0 0 0 0 0.05 0.15 0.15 0.13 – 0.11 0.2 0 0 0 0 0.06 0.05 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0.06 – 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 1 0 7 16 12 – 10 21 0 0 0 0 0 0 0 1 0 0 0 0
Bud 0 1 2 0 2 0 1 1 1 – 1 1 0 1 3 0 1 0 0 3 2 1 0 3
Bud Neck 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 2 7 1 12 14 31 25 16 – 36 42 2 2 2 3 8 5 0 1 0 0 0 0
Cytoplasm 120 436 368 244 255 221 339 315 232 – 210 235 262 356 355 241 264 256 131 425 362 191 211 321
Endoplasmic Reticulum 0 4 7 2 8 11 63 60 39 – 31 65 2 2 6 9 17 14 1 1 6 2 7 9
Endosome 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 1 0 0 0
Golgi 0 1 0 0 0 0 0 3 2 – 2 2 0 0 0 1 0 1 0 0 0 0 0 0
Mitochondria 0 0 0 1 0 0 6 6 19 – 13 14 0 0 0 3 0 1 0 0 0 0 0 0
Nucleus 0 0 0 0 0 1 0 1 1 – 1 0 0 0 0 0 0 0 0 1 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 – 1 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 1 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 1 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 1 0 0 0 0 0 2 0 – 1 1 0 2 0 2 2 0 0 4 4 3 0 0
Unique Cell Count 120 444 373 247 268 239 415 401 298 273 332 264 358 361 248 270 258 135 442 380 203 224 339
Labelled Cell Count 120 447 384 248 278 247 447 429 322 306 381 266 363 366 259 292 277 135 442 380 203 224 339


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 107.7 104.0 66.2 63.5 55.5 48.3 44.4 40.8 36.9 – 34.4 33.3 87.9 82.7 68.9 115.2 121.7 124.7 89.6 89.4 96.1
Std Deviation (1e-4) 24.1 21.3 13.5 12.5 12.0 10.7 9.0 9.3 8.7 – 7.8 7.9 15.8 18.3 15.9 31.1 38.5 39.5 23.7 25.4 23.2
Intensity Change (Log2) – – – -0.06 -0.25 -0.45 -0.58 -0.7 -0.84 – -0.94 -0.99 0.41 0.32 0.06 0.8 0.88 0.91 0.44 0.43 0.54


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 3.9 3.9 – 0 4.9 0 0 0 0 0 0
Bud 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Cell Periphery 0 1.9 2.6 3.7 3.0 2.5 – 5.7 5.6 0 0 0 0 0.9 0
Cytoplasm 0.1 -2.7 -3.9 -7.8 -8.7 -8.7 – -8.9 -10.5 0.7 1.1 -0.4 -1.3 -0.9 0.7
Endoplasmic Reticulum 0 0.9 1.9 6.6 6.5 5.7 – 5.1 7.7 0 0 -0.2 1.3 2.9 2.4
Endosome 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 4.9 – 4.3 4.0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 65.735 110.5874 99.6922 94.3489 96.2217 99.5278 74.6424 120.4267 109.0319 104.829 70.54 107.1728 90.4277 116.6255 104.0802 105.3266 93.3884 115.525
Actin 0.0317 0.0003 0.0004 0.0003 0.0001 0.0015 0.0244 0.0004 0.0005 0.0009 0.0023 0.0024 0.0026 0.0003 0.0138 0.0367 0.0054 0.0003
Bud 0.0021 0.0006 0.0016 0.0019 0.0013 0.001 0.0015 0.0002 0.0014 0.0013 0.0007 0.0004 0.0009 0.0007 0.0009 0.0006 0.0011 0.0002
Bud Neck 0.0029 0.001 0.0016 0.0016 0.0015 0.0091 0.0014 0.0004 0.0009 0.0012 0.0006 0.0013 0.0005 0.0004 0.0007 0.0004 0.0008 0.0019
Bud Periphery 0.003 0.0005 0.0022 0.0022 0.0021 0.0008 0.0017 0.0002 0.0015 0.0006 0.0005 0.0004 0.001 0.001 0.0008 0.0004 0.0009 0.0002
Bud Site 0.0034 0.0011 0.0009 0.0004 0.0004 0.0002 0.0037 0.0003 0.0028 0.0005 0.0008 0.0004 0.0007 0.0009 0.0006 0.0009 0.0004 0.0001
Cell Periphery 0.0524 0.0147 0.051 0.0469 0.0416 0.0109 0.0051 0.0109 0.0188 0.0072 0.0034 0.0096 0.0106 0.0052 0.0022 0.012 0.001 0.0023
Cytoplasm 0.5029 0.9003 0.734 0.5659 0.6657 0.7942 0.7448 0.9515 0.8953 0.797 0.6877 0.8918 0.8005 0.9652 0.8785 0.8788 0.8112 0.9481
Cytoplasmic Foci 0.0103 0.0003 0.0015 0.0007 0.001 0.0021 0.0174 0.0005 0.0016 0.0183 0.0349 0.0019 0.0135 0.0004 0.0094 0.0014 0.0201 0.0017
Eisosomes 0.0016 0 0.0001 0 0 0 0.0007 0.0001 0 0.0004 0.0005 0 0.0002 0 0.0002 0.0001 0.0002 0
Endoplasmic Reticulum 0.0578 0.0054 0.0136 0.0228 0.0012 0.0648 0.0148 0.0044 0.0062 0.0018 0.0095 0.02 0.0233 0.0057 0.0052 0.0009 0.0053 0.0197
Endosome 0.0146 0.0006 0.0017 0.0033 0.0016 0.0063 0.0113 0.0004 0.0016 0.0022 0.0294 0.0044 0.0044 0.0004 0.0068 0.0017 0.0121 0.004
Golgi 0.0033 0.0001 0.0002 0.0001 0.0001 0.0007 0.0044 0.0001 0.0002 0.0003 0.0037 0.0006 0.0008 0.0001 0.0019 0.0006 0.0082 0.0003
Lipid Particles 0.0101 0.0004 0.0021 0.0014 0.0004 0.0006 0.0089 0.0006 0.0011 0.0077 0.036 0.0013 0.0048 0.0003 0.0065 0.0013 0.0161 0.0002
Mitochondria 0.0325 0.0005 0.0073 0.0005 0.0005 0.0009 0.0192 0.0006 0.0009 0.004 0.031 0.0009 0.0066 0.0002 0.0062 0.0005 0.0197 0.0002
None 0.0088 0.0003 0.0013 0.0003 0.0006 0.0004 0.0083 0.0003 0.0012 0.0153 0.0156 0.0015 0.0111 0.0002 0.0071 0.0002 0.0153 0.0002
Nuclear Periphery 0.0495 0.0068 0.0213 0.0305 0.0039 0.0173 0.0219 0.0038 0.0036 0.0042 0.0341 0.0123 0.0137 0.0019 0.0059 0.0031 0.007 0.0032
Nucleolus 0.0243 0.0009 0.0069 0.0009 0.0006 0.0002 0.0044 0.0015 0.0008 0.0066 0.0172 0.0015 0.0091 0.0001 0.0017 0.0003 0.0037 0
Nucleus 0.0314 0.0059 0.0112 0.016 0.0055 0.0068 0.0124 0.0031 0.0034 0.0056 0.0107 0.0113 0.0123 0.0017 0.0051 0.0025 0.0041 0.0017
Peroxisomes 0.0021 0 0.0004 0.0001 0.0001 0.0001 0.0038 0 0.0001 0.0005 0.0056 0.0002 0.0022 0 0.002 0.0003 0.009 0
Punctate Nuclear 0.0086 0 0.0001 0.0001 0 0.0001 0.0123 0.0004 0.0001 0.0009 0.0032 0.0051 0.0039 0 0.003 0.0001 0.0008 0
Vacuole 0.121 0.0559 0.1219 0.2356 0.2501 0.0696 0.0672 0.0193 0.0545 0.1104 0.053 0.0281 0.0618 0.0147 0.0326 0.0516 0.0424 0.0136
Vacuole Periphery 0.0258 0.0043 0.0189 0.0686 0.0218 0.0125 0.0105 0.0011 0.0034 0.0131 0.0197 0.0046 0.0155 0.0007 0.0089 0.0057 0.0152 0.0021

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 411.5715 447.5143 472.721 421.8083 408.6847 407.8507 420.967 371.4886 385.8672 372.3785
Translational Efficiency 1.1444 1.1484 1.0082 1.2251 1.0143 0.987 0.9891 0.9115 0.8553 0.8464

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1779 2025 2286 387 1727 1510 100 565 3506 3535 2386 952

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 4756.91 6579.81 9388.02 5456.66 4971.01 5583.45 6661.59 6694.19 4862.37 6154.21 9273.75 6191.12
Standard Deviation 859.70 1323.65 1516.70 1106.14 1306.40 1779.56 971.90 1543.39 1107.77 1612.23 1594.37 1510.16
Intensity Change Log 2 — 0.468021 0.980796 0.197993 — 0.167618 0.422328 0.429370 — 0.322327 0.722335 0.320854

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000021 0.000048 0.000022 0.000351 0.000030 0.000103 0.000235 0.000070 0.000026 0.000072 0.000031 0.000184
Bud Neck 0.000090 0.000246 0.000171 0.002408 0.000152 0.000532 0.006194 0.001376 0.000121 0.000368 0.000424 0.001795
Bud Site 0.001022 0.001555 0.000948 0.014619 0.000988 0.002827 0.004761 0.013687 0.001006 0.002099 0.001108 0.014066
Cell Periphery 0.003172 0.009050 0.002275 0.041609 0.007883 0.007921 0.053199 0.024113 0.005493 0.008568 0.004409 0.031226
Cytoplasm 0.932981* 0.894980* 0.944135* 0.623798* 0.869874* 0.872117* 0.316761* 0.824378* 0.901896* 0.885214* 0.917841* 0.742839*
Cytoplasmic Foci 0.004013 0.004802 0.002249 0.006303 0.003616 0.006172 0.011817 0.001010 0.003817 0.005387 0.002650 0.003162
Eisosomes 0.000000 0.000001 0.000000 0.000005 0.000001 0.000001 0.000010 0.000003 0.000000 0.000001 0.000001 0.000004
Endoplasmic Reticulum 0.000404 0.000315 0.000183 0.000177 0.000550 0.000347 0.000144 0.000090 0.000476 0.000328 0.000181 0.000126
Endosome 0.000179 0.000249 0.000102 0.000528 0.000265 0.000354 0.000658 0.000143 0.000221 0.000294 0.000125 0.000299
Golgi 0.015498 0.022234 0.005576 0.013868 0.024998 0.020094 0.017922 0.000331 0.020177 0.021320 0.006094 0.005834
Lipid Particles 0.002607 0.009647 0.001517 0.030370 0.006269 0.007176 0.026080 0.000338 0.004410 0.008591 0.002546 0.012547
Mitochondria 0.000090 0.000093 0.000119 0.000119 0.000097 0.000163 0.000084 0.000259 0.000094 0.000123 0.000117 0.000202
Mitotic Spindle 0.000331 0.000434 0.000275 0.001049 0.000304 0.001133 0.000718 0.000014 0.000318 0.000733 0.000294 0.000435
None 0.001474 0.001673 0.001300 0.001002 0.003077 0.009016 0.000656 0.000507 0.002264 0.004809 0.001273 0.000709
Nuclear Periphery 0.000117 0.000291 0.000217 0.000412 0.000335 0.000328 0.002133 0.000007 0.000224 0.000307 0.000297 0.000172
Nuclear Periphery Foci 0.003383 0.006766 0.002416 0.005496 0.008106 0.007464 0.010811 0.000022 0.005710 0.007064 0.002768 0.002247
Nucleolus 0.000020 0.000024 0.000027 0.000254 0.000049 0.000086 0.000145 0.000151 0.000034 0.000051 0.000032 0.000193
Nucleus 0.004946 0.006998 0.007375 0.018704 0.007021 0.022338 0.021551 0.001095 0.005968 0.013550 0.007970 0.008254
Peroxisomes 0.000007 0.000013 0.000012 0.000073 0.000013 0.000012 0.000021 0.000281 0.000010 0.000013 0.000013 0.000196
Vacuole 0.011739 0.025785 0.019523 0.223115* 0.032895 0.028521 0.497240* 0.125016 0.022160 0.026954 0.039544 0.164895
Vacuole Periphery 0.017905 0.014797 0.011556 0.015740 0.033477 0.013295 0.028861 0.007109 0.025576 0.014156 0.012281 0.010618

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -4.69 -1.43 -6.04 -5.46 -5.97 -2.27 -4.98 -5.91 1.24 4.29 -3.32 -2.77 -6.79 -3.93 -6.32
Bud Neck -3.67 -3.94 -10.24 -9.39 -9.61 -6.40 -5.76 -3.91 -2.39 4.93 -6.94 -5.95 -8.83 -7.31 -6.03
Bud Site -2.90 0.16 -5.51 -5.26 -5.52 -4.62 -3.11 -13.16 -10.32 -3.86 -5.45 -1.58 -11.29 -10.15 -11.08
Cell Periphery -8.35 1.72 -9.90 -8.09 -10.13 -0.17 -4.30 -5.00 -4.94 2.16 -5.32 2.38 -10.12 -8.72 -10.64
Cytoplasm 6.70 -1.33 19.77 17.15 20.35 0.21 17.15 3.81 3.68 -15.30 3.93 -2.19 16.40 14.42 17.16
Cytoplasmic Foci -1.69 3.89 -2.50 -1.84 -3.83 -5.08 -3.43 10.79 10.13 3.99 -4.73 2.00 0.43 3.32 -0.74
Eisosomes -4.40 -1.22 -11.55 -9.58 -11.19 -4.05 -6.29 -9.88 -5.38 5.19 -5.66 -3.10 -14.94 -10.99 -11.90
Endoplasmic Reticulum 0.84 2.00 2.12 4.17 0.53 1.67 3.89 4.57 3.67 3.09 1.84 3.85 4.70 5.81 2.98
Endosome -2.32 2.82 -3.74 -3.03 -4.61 -1.91 -4.67 4.64 4.70 5.77 -2.75 4.27 -1.90 -0.06 -4.09
Golgi -4.15 8.44 1.55 4.94 -4.25 2.61 4.33 18.07 16.15 10.95 -0.99 14.86 13.53 14.72 1.21
Lipid Particles -7.30 2.22 -10.86 -7.62 -11.28 -1.12 -6.61 8.38 9.65 8.84 -5.99 3.60 -6.74 -2.88 -8.42
Mitochondria -0.39 -2.64 -0.71 -0.59 0.13 -1.61 -0.65 -3.67 -1.30 -3.07 -1.63 -3.21 -3.54 -1.98 -2.64
Mitotic Spindle -2.20 0.59 -1.46 -1.27 -1.52 -2.37 -1.26 10.12 3.09 1.43 -2.70 -0.89 -0.68 1.13 -0.34
None -1.67 1.52 2.02 2.85 1.37 -3.94 5.95 4.89 5.69 0.50 -3.88 4.72 5.18 6.17 2.46
Nuclear Periphery -6.85 -2.50 -6.80 -2.71 -2.71 -0.36 -5.22 10.87 10.65 6.20 -3.85 -1.91 1.66 5.07 2.64
Nuclear Periphery Foci -5.78 1.94 -4.11 1.79 -5.64 0.47 -1.76 10.26 11.89 8.93 -2.52 5.65 7.38 10.70 2.11
Nucleolus -2.26 -3.42 -2.63 -2.57 -2.52 -3.01 -4.50 -8.71 -3.46 0.96 -3.04 -0.34 -4.33 -3.76 -4.30
Nucleus -2.19 -3.31 -5.32 -4.75 -4.28 -7.96 -4.74 8.80 11.46 5.80 -7.80 -4.11 -2.53 2.87 0.01
Peroxisomes -3.59 -3.01 -7.51 -6.73 -6.85 0.12 -2.30 -10.59 -10.61 -9.63 -2.19 -1.83 -11.73 -11.54 -11.52
Vacuole -7.18 -5.47 -17.84 -16.59 -16.98 1.05 -14.44 -10.54 -10.94 11.18 -3.28 -6.40 -19.87 -19.04 -16.47
Vacuole Periphery 1.74 3.71 1.65 0.27 -1.45 8.22 2.12 11.51 5.19 4.72 7.55 8.63 10.21 4.00 2.05
Standard name
Human Ortholog
Description Mitochondrial and cytoplasmic valyl-tRNA synthetase; human homolog VARS2 implicated in mitochondrial diseases, can partially complement yeast null mutant
Localization
Cell Percentages cytoplasm (100%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-2

Vas1

Vas1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Vas1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available