Standard name
Human Ortholog
Description Cytoplasmic GTPase-activating protein; activates Ypt/Rab transport GTPases Ypt6p, Ypt31p and Sec4p; involved in recycling of internalized proteins and regulation of Golgi secretory function
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.08 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0.09 0.09 0.08 0.06 0 0.05 0.14 0.16 0.19
Bud 0 0 0.06 0.05 0.05 0.09 0 0.05 0.08 0.06 0.08 0.07 0.05 0 0.06 0 0 0 0 0 0 0.07 0.05 0.05
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.62 0.85 0.73 0.64 0.68 0.6 0.65 0.55 0.53 0.5 0.49 0.41 0.86 0.88 0.88 0.56 0.53 0.54 0.41 0.62 0.28 0.25 0.27 0.23
Endoplasmic Reticulum 0.13 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0.05 0.08 0 0.05 0.08 0.08 0.13
Endosome 0.13 0 0.05 0 0.11 0 0.07 0.06 0 0 0 0 0 0 0 0.19 0.19 0.19 0.17 0.07 0.11 0.11 0.09 0.11
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0.1 0 0 0 0 0.07
Mitochondria 0 0.22 0.16 0.36 0.2 0.4 0.38 0.48 0.59 0.62 0.63 0.69 0.05 0 0.22 0.05 0 0 0.06 0.07 0.33 0.16 0.09 0.12
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.1 0.09 0 0 0 0 0 0
Vac/Vac Membrane 0.1 0 0 0 0 0 0 0.07 0 0 0 0 0 0 0 0.08 0.08 0.08 0 0.1 0.07 0.05 0.07 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 3 0 6 12 6 5 3 2 5 0 4 2 1 2 0 28 21 29 6 3 16 7 20 24
Bud 0 1 17 13 17 29 17 31 25 21 26 35 2 3 8 3 2 5 2 1 4 3 6 6
Bud Neck 0 0 1 0 0 0 0 1 0 0 1 0 0 0 0 0 2 0 1 3 5 1 7 3
Bud Site 0 0 0 0 1 1 2 3 3 4 3 4 0 0 0 0 0 0 – – – – – –
Cell Periphery 1 0 2 4 10 2 10 5 6 10 8 7 0 0 0 12 6 13 0 0 0 0 0 0
Cytoplasm 24 47 193 168 215 196 314 342 171 164 168 196 36 70 114 177 121 184 40 57 92 12 36 30
Endoplasmic Reticulum 5 2 10 7 6 11 4 9 6 6 10 11 1 2 2 19 9 17 7 1 15 3 11 16
Endosome 5 0 14 7 35 14 32 37 3 3 0 1 1 2 2 61 43 66 17 6 36 5 12 14
Golgi 1 0 1 0 0 0 0 1 0 0 0 2 1 1 0 9 12 14 9 0 6 1 4 8
Mitochondria 1 12 41 95 62 132 185 298 188 205 214 334 2 2 28 16 9 13 5 6 108 8 11 15
Nucleus 1 0 1 0 0 0 5 0 1 1 2 3 1 0 1 2 1 4 0 0 0 1 0 0
Nuclear Periphery 0 1 0 0 0 0 0 2 0 0 1 0 0 0 0 1 0 0 1 0 2 0 0 0
Nucleolus 0 0 0 0 0 0 3 2 0 0 1 1 0 2 1 1 1 1 0 0 0 0 0 0
Peroxisomes 0 0 3 1 5 2 6 5 1 1 0 1 0 0 0 0 1 0 0 0 2 0 1 1
SpindlePole 1 0 5 7 14 10 14 17 3 1 6 14 0 0 0 17 22 32 1 1 8 0 3 1
Vac/Vac Membrane 4 1 4 1 3 6 9 41 3 5 5 14 0 1 0 26 18 26 4 8 21 2 8 2
Unique Cell Count 39 55 263 264 315 328 483 620 320 331 342 483 42 80 129 316 229 342 99 92 326 50 135 130
Labelled Cell Count 46 64 298 315 374 408 604 796 415 421 449 625 45 85 156 372 268 404 99 92 326 50 135 130


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 8.4 4.8 5.1 4.9 5.2 4.7 5.0 4.5 4.3 4.4 4.3 4.2 6.5 7.2 6.5 8.5 9.2 9.5 5.9 7.2 8.0
Std Deviation (1e-4) 1.0 0.7 0.9 1.6 1.2 1.2 1.6 1.1 1.0 1.4 1.0 1.1 1.1 1.0 1.0 1.2 1.4 1.6 1.5 2.0 1.8
Intensity Change (Log2) – – – -0.04 0.03 -0.11 -0.03 -0.17 -0.23 -0.19 -0.24 -0.26 0.36 0.5 0.36 0.74 0.86 0.9 0.21 0.52 0.66


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – 0 0 0
Bud Neck – – – – – – – – – – – – 0 0 0
Bud Site – – – – – – – – – – – – 0 0 0
Cell Periphery – – – – – – – – – – – – 0 0 0
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – 0 0 0
Endosome – – – – – – – – – – – – 0 0 0
Golgi – – – – – – – – – – – – 0 0 0
Mitochondria – – – – – – – – – – – – 0 0 0
Nuclear Periphery – – – – – – – – – – – – 0 0 0
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – 0 0 0
Peroxisomes – – – – – – – – – – – – 0 0 0
SpindlePole – – – – – – – – – – – – 0 0 0
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – 0 0 0
Cytoplasm – – – – – – – – – – – – 0 0 0
Nucleus – – – – – – – – – – – – 0 0 0
Vacuole – – – – – – – – – – – – 3.6 3.4 3.4

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 2.7623 3.4122 3.1103 2.5279 2.7073 3.0593 2.042 3.0316 2.7337 2.6234 2.3402 3.0537 3.8319 4.7483 4.1961 3.8676 4.1569 4.1512
Actin 0.0231 0.0093 0.0209 0.0023 0.0102 0.0121 0.0194 0.0069 0.0166 0.0115 0.0018 0.0092 0.0043 0.0168 0.0145 0.0028 0.0086 0.0095
Bud 0.0032 0.0028 0.002 0.0176 0.0018 0.0019 0.0011 0.0005 0.0017 0.0009 0.0024 0.0003 0.0016 0.0028 0.0021 0.0006 0.0014 0.004
Bud Neck 0.0065 0.0016 0.0033 0.0018 0.0016 0.0194 0.0057 0.0015 0.0009 0.0006 0.0015 0.0139 0.0013 0.0007 0.0048 0.0005 0.0007 0.004
Bud Periphery 0.0056 0.0018 0.002 0.0101 0.0014 0.0031 0.0014 0.0004 0.0015 0.0012 0.0023 0.0004 0.0015 0.001 0.001 0.0004 0.0018 0.0042
Bud Site 0.0294 0.0359 0.0065 0.0041 0.0021 0.0027 0.0081 0.0059 0.0118 0.0021 0.0097 0.0003 0.0502 0.0391 0.0099 0.0007 0.004 0.0005
Cell Periphery 0.0035 0.0015 0.0014 0.0033 0.0004 0.0008 0.0009 0.0006 0.0005 0.0003 0.0004 0.0002 0.0007 0.0008 0.0005 0.0003 0.0005 0.0004
Cytoplasm 0.5259 0.6934 0.6725 0.6734 0.5129 0.6471 0.5649 0.7522 0.6304 0.5674 0.7053 0.6892 0.6074 0.6676 0.6196 0.6449 0.6186 0.6451
Cytoplasmic Foci 0.0602 0.0469 0.0633 0.0237 0.0956 0.0704 0.081 0.0691 0.0789 0.0842 0.0551 0.0648 0.0691 0.0508 0.1001 0.0894 0.0656 0.0739
Eisosomes 0.0006 0.0001 0.0003 0.0001 0.0001 0.0001 0.0002 0.0002 0.0002 0.0001 0 0.0001 0.0002 0.0001 0.0001 0.0001 0.0002 0.0001
Endoplasmic Reticulum 0.0371 0.0146 0.0094 0.0135 0.0176 0.0178 0.0167 0.0041 0.0047 0.0038 0.003 0.0074 0.0106 0.0042 0.0101 0.0321 0.0063 0.0102
Endosome 0.132 0.1121 0.1202 0.1215 0.1962 0.1293 0.1209 0.058 0.0991 0.1435 0.0999 0.0793 0.1574 0.0723 0.1505 0.123 0.1075 0.123
Golgi 0.038 0.0305 0.0224 0.0059 0.0443 0.0329 0.0335 0.0116 0.0184 0.0182 0.0286 0.0235 0.035 0.0131 0.0221 0.0173 0.0264 0.0396
Lipid Particles 0.0097 0.001 0.0061 0.0007 0.0168 0.0039 0.0093 0.0018 0.0081 0.0048 0.0047 0.0055 0.0013 0.0005 0.0015 0.0008 0.0205 0.0006
Mitochondria 0.0499 0.008 0.0266 0.0143 0.0352 0.0298 0.0102 0.0017 0.0159 0.0106 0.0053 0.0066 0.0046 0.0028 0.0062 0.0084 0.0065 0.0178
None 0.0261 0.0218 0.0199 0.0733 0.0349 0.0105 0.0929 0.0742 0.0935 0.1131 0.0642 0.0876 0.0422 0.1204 0.0378 0.0674 0.1082 0.0568
Nuclear Periphery 0.0055 0.0015 0.002 0.0015 0.0015 0.0016 0.0085 0.0006 0.0008 0.0017 0.0007 0.0005 0.0016 0.0006 0.0037 0.0012 0.0038 0.0006
Nucleolus 0.002 0.0003 0.0004 0.001 0.0005 0.0003 0.0005 0.0005 0.0002 0.0002 0.0004 0.0001 0.0001 0.0001 0.0011 0.0001 0.0012 0.0001
Nucleus 0.0031 0.0015 0.0014 0.0086 0.0013 0.0012 0.0047 0.0009 0.0009 0.0012 0.0017 0.0005 0.0014 0.0009 0.0013 0.0008 0.0017 0.0006
Peroxisomes 0.0072 0.0006 0.0023 0.0004 0.0101 0.0029 0.0069 0.0028 0.0047 0.009 0.0027 0.0062 0.0012 0.0007 0.004 0.0011 0.0061 0.0026
Punctate Nuclear 0.0046 0.0009 0.0019 0.0004 0.0016 0.0008 0.0041 0.0012 0.001 0.0011 0.0008 0.0006 0.0012 0.0004 0.0014 0.0003 0.0046 0.0002
Vacuole 0.0212 0.0115 0.0124 0.0166 0.01 0.008 0.0075 0.0044 0.0083 0.0175 0.0078 0.0026 0.0053 0.0035 0.0058 0.0058 0.0044 0.0047
Vacuole Periphery 0.0057 0.0025 0.0031 0.0057 0.004 0.0033 0.0017 0.0007 0.002 0.0071 0.0018 0.0012 0.0018 0.0008 0.0021 0.0019 0.0014 0.0016

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 11.0224 7.6061 12.3143 19.1278 14.0404 12.7558 17.2163 19.9366 22.0451 20.6851
Translational Efficiency 0.6029 0.7542 0.7376 0.584 0.5098 0.6295 0.538 0.3637 0.5311 0.4709

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
490 1455 425 914 1321 2157 309 70 1811 3612 734 984

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 747.42 874.51 1427.04 966.71 734.14 880.23 1160.57 1020.68 737.73 877.93 1314.86 970.55
Standard Deviation 94.63 120.74 177.97 134.88 130.16 115.53 133.76 150.04 121.72 117.69 207.80 136.72
Intensity Change Log 2 — 0.226556 0.933035 0.371164 — 0.261825 0.660706 0.475404 — 0.244140 0.804503 0.423758

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000638 0.001539 0.009173 0.006078 0.001002 0.001151 0.007321 0.017415 0.000903 0.001307 0.008393 0.006884
Bud Neck 0.017988 0.006229 0.005420 0.010239 0.007303 0.008637 0.007202 0.006761 0.010194 0.007667 0.006170 0.009992
Bud Site 0.029343 0.035336 0.122386* 0.075003 0.024393 0.032283 0.061735 0.150428* 0.025732 0.033512 0.096853 0.080368
Cell Periphery 0.000317 0.000394 0.000658 0.000140 0.000240 0.000168 0.000166 0.000329 0.000261 0.000259 0.000451 0.000153
Cytoplasm 0.293076* 0.239072* 0.431431* 0.276295* 0.248525* 0.255248* 0.300344* 0.194548* 0.260579* 0.248732* 0.376246* 0.270480*
Cytoplasmic Foci 0.384492* 0.456584* 0.210376* 0.228884* 0.435588* 0.412325* 0.232816* 0.303143* 0.421763* 0.430153* 0.219823* 0.234167*
Eisosomes 0.000461 0.000149 0.000035 0.000078 0.000203 0.000204 0.000072 0.000125 0.000273 0.000182 0.000051 0.000081
Endoplasmic Reticulum 0.004271 0.003421 0.005113 0.002410 0.007359 0.002758 0.004087 0.002128 0.006523 0.003025 0.004681 0.002390
Endosome 0.081529 0.077977 0.041423 0.080871 0.084543 0.082701 0.045098 0.066688 0.083728 0.080798 0.042970 0.079862
Golgi 0.062993 0.088132 0.037314 0.014752 0.070488 0.093749 0.005502 0.034338 0.068460 0.091486 0.023922 0.016146
Lipid Particles 0.014173 0.004872 0.012430 0.004717 0.015408 0.005549 0.007084 0.008233 0.015074 0.005276 0.010179 0.004967
Mitochondria 0.018315 0.010168 0.000095 0.005748 0.015207 0.016012 0.000206 0.000150 0.016048 0.013657 0.000142 0.005350
Mitotic Spindle 0.001005 0.003925 0.037642 0.038997 0.001569 0.005479 0.024820 0.010475 0.001417 0.004853 0.032244 0.036968
None 0.000444 0.000243 0.000142 0.001614 0.003169 0.000247 0.000297 0.000897 0.002431 0.000245 0.000207 0.001563
Nuclear Periphery 0.000200 0.000098 0.000155 0.000251 0.000655 0.000099 0.001171 0.000111 0.000532 0.000098 0.000583 0.000241
Nuclear Periphery Foci 0.000334 0.000780 0.008146 0.000947 0.001141 0.000181 0.008387 0.003035 0.000923 0.000422 0.008247 0.001095
Nucleolus 0.000256 0.000302 0.000039 0.000387 0.001712 0.000230 0.000232 0.000075 0.001318 0.000259 0.000121 0.000365
Nucleus 0.006316 0.002337 0.004912 0.010667 0.009525 0.002455 0.023526 0.005942 0.008657 0.002408 0.012748 0.010331
Peroxisomes 0.009458 0.006710 0.002687 0.025588 0.005065 0.005711 0.013032 0.035211 0.006253 0.006114 0.007042 0.026272
Vacuole 0.055825 0.047278 0.066688 0.211973* 0.048180 0.056411 0.255903* 0.157225 0.050249 0.052732 0.146344 0.208078*
Vacuole Periphery 0.018567 0.014455 0.003735 0.004363 0.018727 0.018404 0.000998 0.002744 0.018684 0.016813 0.002582 0.004248

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -3.31 -5.08 -4.31 -3.38 2.00 -0.61 -3.58 -2.14 -2.12 -1.39 -2.04 -6.07 -4.56 -4.20 1.19
Bud Neck 5.83 6.23 3.81 -3.88 -4.90 -1.59 0.40 0.89 1.86 0.46 3.12 4.77 0.44 -2.42 -4.02
Bud Site -1.58 -9.43 -6.67 -6.56 5.43 -4.04 -4.72 -4.68 -4.35 -3.40 -4.48 -10.93 -9.88 -8.28 2.83
Cell Periphery -0.33 -4.99 5.37 1.16 8.52 2.99 3.42 -0.85 -1.96 -2.00 0.03 -4.80 5.62 1.28 7.94
Cytoplasm 4.13 -7.39 0.59 -4.02 8.37 -0.84 -3.28 1.37 1.61 2.85 1.70 -9.49 -1.76 -3.08 6.97
Cytoplasmic Foci -6.58 14.37 15.97 29.02 0.44 3.23 20.01 6.12 5.27 -2.88 -1.39 27.02 26.39 30.06 -0.37
Eisosomes 3.55 4.95 4.53 3.77 -4.33 -0.04 7.41 3.22 3.20 -2.30 3.09 8.32 7.25 7.24 -3.21
Endoplasmic Reticulum 0.81 -0.70 2.06 1.68 3.25 4.65 2.63 5.18 1.26 2.55 4.49 2.04 4.91 1.53 3.51
Endosome 0.66 7.63 2.56 2.55 -5.67 0.53 9.84 2.62 2.47 -2.25 0.99 13.16 4.16 3.86 -6.48
Golgi -4.40 4.90 10.51 22.86 6.51 -6.24 22.70 4.17 6.50 -2.52 -7.49 14.99 20.67 32.43 3.70
Lipid Particles 5.80 0.49 6.01 0.71 6.98 7.82 6.29 2.93 -1.21 -0.54 9.67 3.70 9.59 1.08 6.83
Mitochondria 3.12 7.39 5.03 3.79 -3.42 -0.55 12.54 12.60 19.20 1.49 1.91 14.49 7.46 7.27 -3.39
Mitotic Spindle -3.30 -7.97 -9.08 -8.37 -0.45 -3.89 -5.03 -2.22 -1.13 2.82 -4.90 -9.31 -9.12 -8.22 -0.97
None 1.50 3.44 -0.87 -1.06 -1.18 3.49 3.45 2.21 -0.71 -0.68 3.56 3.65 1.08 -1.11 -1.16
Nuclear Periphery 1.38 0.21 -2.16 -7.13 -5.05 3.30 -1.30 3.06 -0.79 2.54 3.47 -0.50 1.48 -8.19 1.56
Nuclear Periphery Foci -1.17 -9.65 -4.84 -0.72 8.87 6.72 -7.01 -1.61 -2.38 3.43 2.70 -11.46 -1.79 -4.08 11.01
Nucleolus -0.40 8.90 -2.98 -0.60 -6.93 3.75 3.73 4.19 3.11 4.34 3.54 4.15 3.14 -1.47 -5.07
Nucleus 4.33 0.04 -4.17 -7.14 -4.38 6.90 -4.84 0.14 -1.22 3.14 7.90 -3.78 -2.81 -7.26 1.06
Peroxisomes 2.28 6.13 -5.13 -6.88 -9.01 -1.36 -3.20 -4.17 -4.07 -3.17 0.29 0.20 -7.91 -8.05 -7.57
Vacuole 1.66 -2.52 -20.92 -23.60 -17.83 -2.70 -17.30 -5.25 -4.96 3.08 -1.05 -14.35 -23.88 -23.81 -7.52
Vacuole Periphery 2.16 8.28 8.16 11.54 0.15 0.24 17.81 12.81 13.78 -1.86 1.78 17.87 14.86 16.61 -1.51
Standard name
Human Ortholog
Description Cytoplasmic GTPase-activating protein; activates Ypt/Rab transport GTPases Ypt6p, Ypt31p and Sec4p; involved in recycling of internalized proteins and regulation of Golgi secretory function
Localization
Cell Percentages cytoplasm (52%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-10

Mdr1

Mdr1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Mdr1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available