Standard name
Human Ortholog
Description Component of several different pre-ribosomal particles; forms a complex with Ytm1p and Erb1p that is required for maturation of the large ribosomal subunit; required for exit from G<sub>0</sub> and the initiation of cell proliferation
DOWNLOAD

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 – – – 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 – 0 0 0 0 – – – 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 – – – 0 0 0 0 0 0 0 0 0.05
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0.05 – – – 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 – 0 0 0 0 – – – 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0.06 0 0 0 0 0 – 0 0 0 0 – – – 0 0 0 0 0 0 0.07 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 – 0 0 0 0 – – – 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0.1 – 0 0 0 0 – – – 0 0 0 0 0 0 0 0.05 0.09
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 – – – 0 0 0 0 0 0 0 0 0
Mitochondria 0.11 0.1 0 0.12 0 0.14 0.08 – 0.14 0.1 0.19 0.09 – – – 0.09 0.17 0.07 0 0 0 0 0 0
Nucleus 0.4 0.35 0.4 0 0.08 0.08 0.1 – 0.12 0.13 0.25 0.17 – – – 0.16 0.13 0.15 0.2 0.27 0.25 0 0.06 0.11
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 – – – 0 0 0 0 0 0 0.05 0 0
Nucleolus 0.62 0.58 0.62 0.98 0.88 0.83 0.83 – 0.76 0.76 0.69 0.76 – – – 0.83 0.8 0.8 0.69 0.64 0.63 0.45 0.47 0.41
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 – – – 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0.11 0.14 0.11 0.14 – 0.13 0.14 0.13 0.14 – – – 0 0 0 0 0 0 0.29 0.17 0.1
Vac/Vac Membrane 0 0.05 0 0 0 0 0 – 0 0 0 0 – – – 0 0 0 0 0 0 0 0.09 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 – – – 0 0 0 1 0 0 0 0 0
Bud 0 0 0 0 0 0 0 – 1 0 0 2 – – – 0 0 0 0 2 1 0 0 0
Bud Neck 0 0 0 2 1 0 0 – 1 1 1 0 – – – 0 0 0 1 0 0 0 0 1
Bud Site 0 0 0 0 0 0 0 – 2 2 3 10 – – – 0 0 1 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 – 0 0 0 0 – – – 0 0 0 0 0 0 0 0 0
Cytoplasm 5 18 3 2 1 3 7 – 2 5 1 2 – – – 6 7 6 1 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 – 0 0 0 0 – – – 1 3 1 0 0 0 0 0 0
Endosome 1 0 0 6 8 6 27 – 5 6 1 8 – – – 0 0 2 1 2 2 0 0 2
Golgi 0 0 0 0 1 0 0 – 0 0 0 0 – – – 1 0 0 0 0 0 0 0 0
Mitochondria 28 28 1 22 8 22 23 – 19 18 26 19 – – – 18 31 12 3 1 1 0 0 0
Nucleus 98 98 42 4 15 13 27 – 16 25 33 35 – – – 30 24 27 54 83 35 0 1 3
Nuclear Periphery 1 0 0 0 0 3 2 – 1 4 2 3 – – – 0 0 0 3 2 0 0 0 0
Nucleolus 155 165 66 179 172 135 230 – 103 142 93 159 – – – 158 143 145 192 197 88 5 8 13
Peroxisomes 3 4 1 5 7 4 6 – 3 3 2 1 – – – 1 1 2 0 0 0 0 0 0
SpindlePole 0 1 1 21 27 18 39 – 18 27 17 29 – – – 0 1 2 4 1 4 3 3 3
Vac/Vac Membrane 0 15 1 0 8 4 6 – 3 7 1 7 – – – 4 6 5 9 13 3 0 1 1
Unique Cell Count 248 283 106 183 195 162 276 136 188 134 208 190 178 182 277 308 141 12 19 32
Labelled Cell Count 291 329 115 241 248 208 367 174 240 180 275 219 216 203 277 308 141 12 19 32


Nucleolus, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 21.7 34.0 24.0 23.0 20.0 15.6 14.5 – 12.7 11.5 9.6 10.9 – – – 47.8 56.4 52.2 – – –
Std Deviation (1e-4) 7.5 14.7 7.4 7.0 5.6 4.3 4.2 – 5.4 3.3 2.7 3.4 – – – 23.6 24.2 22.8 – – –
Intensity Change (Log2) – – – -0.06 -0.26 -0.63 -0.73 – -0.92 -1.07 -1.32 -1.13 – – – 0.99 1.23 1.12 – – –


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 – 0 0 0 0 – – – 0 0 0
Bud 0 0 0 0 – 0 0 0 0 – – – 0 0 0
Bud Neck 0 0 0 0 – 0 0 0 0 – – – 0 0 0
Bud Site 0 0 0 0 – 0 0 0 0 – – – 0 0 0
Cell Periphery 0 0 0 0 – 0 0 0 0 – – – 0 0 0
Cytoplasm 0 0 0 0 – 0 0 0 0 – – – 0 0 0
Endoplasmic Reticulum 0 0 0 0 – 0 0 0 0 – – – 0 0 0
Endosome 0 0 0 3.3 – 0 0 0 0 – – – 0 0 0
Golgi 0 0 0 0 – 0 0 0 0 – – – 0 0 0
Mitochondria 3.4 0 3.6 2.7 – 3.7 2.9 4.5 2.8 – – – 2.9 4.2 0
Nucleus -8.4 -6.8 -6.3 -6.8 – -5.0 -5.2 -2.5 -4.4 – – – -4.6 -5.0 -4.8
Nuclear Periphery 0 0 0 0 – 0 0 0 0 – – – 0 0 0
Nucleolus 8.1 5.3 3.9 4.4 – 2.3 2.4 1.2 2.6 – – – 4.0 3.3 3.2
Peroxisomes 0 0 0 0 – 0 0 0 0 – – – 0 0 0
SpindlePole 3.3 3.7 3.2 3.8 – 3.5 3.8 3.4 3.7 – – – 0 0 0
Vacuole 0 0 0 0 – 0 0 0 0 – – – 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 17.4907 25.4308 21.304 20.1886 20.1327 21.0398 18.0732 21.9529 19.2484 16.626 16.3107 18.7259 18.6102 27.0207 23.6623 22.1346 23.1138 23.5554
Actin 0.0184 0 0.0072 0 0.003 0.0001 0.0152 0.0013 0.0092 0.0362 0.0114 0.0004 0.0286 0 0.0136 0 0 0.0002
Bud 0.0003 0 0 0 0.0001 0.0001 0.0004 0.0001 0.0001 0.0016 0.0003 0 0.0006 0 0.0014 0 0 0
Bud Neck 0.0055 0.0001 0.0001 0 0.0007 0.0001 0.0019 0.0012 0.0046 0.0013 0.0004 0.0002 0.0023 0.0001 0.0025 0 0.0001 0.0001
Bud Periphery 0.0004 0 0 0 0.0002 0.0003 0.0006 0.0001 0.0001 0.0018 0.0004 0.0002 0.0008 0.0001 0.0064 0.0002 0 0.0002
Bud Site 0.0063 0.0001 0.0001 0 0.0003 0.0001 0.0066 0.0033 0.001 0.0293 0.0011 0 0.0086 0.0001 0.0011 0 0 0
Cell Periphery 0.0004 0.0002 0.0001 0.0001 0.0001 0.001 0.0004 0.0001 0.0006 0.0004 0.0002 0.0006 0.0003 0.0003 0.0003 0.0002 0 0.0003
Cytoplasm 0.0016 0 0 0.0001 0.0002 0.0001 0.0026 0.0009 0.003 0.0009 0.0021 0.0005 0.0011 0 0.0001 0 0 0
Cytoplasmic Foci 0.0087 0 0.0003 0.0075 0.0062 0.0101 0.0246 0.0024 0.0144 0.0072 0.0122 0.0257 0.0271 0.0001 0.0043 0 0.0001 0.0004
Eisosomes 0.0004 0 0 0 0 0.0001 0.0003 0 0.0001 0.0003 0.0003 0.0002 0.0002 0 0.0001 0 0 0.0001
Endoplasmic Reticulum 0.0007 0 0 0 0.0001 0 0.0103 0.0006 0.0012 0.0004 0.0025 0 0.0027 0 0.0001 0 0 0
Endosome 0.0087 0 0.0022 0 0.0104 0.0001 0.0104 0.0048 0.0017 0.0093 0.0163 0.0005 0.0214 0 0.0003 0.0001 0 0.0001
Golgi 0.0026 0 0.0038 0 0.0244 0.0001 0.0049 0.0014 0.0014 0.0091 0.0107 0.0003 0.0157 0 0.0006 0 0 0.0001
Lipid Particles 0.0101 0.0002 0.0004 0.0018 0.0042 0.006 0.0106 0.0019 0.0079 0.0061 0.0262 0.0407 0.0282 0.0002 0.0015 0.0001 0.0002 0.0097
Mitochondria 0.0118 0.0003 0.001 0.0007 0.0031 0.0121 0.0128 0.0008 0.0014 0.019 0.0194 0.0178 0.0067 0.0005 0.0042 0.0004 0.0002 0.0114
None 0.0015 0 0 0.0003 0.0001 0.0004 0.0028 0.0005 0.0022 0.0004 0.0024 0.0028 0.0016 0 0.0001 0 0 0.0001
Nuclear Periphery 0.0064 0.0064 0.0008 0.0007 0.0258 0.0109 0.011 0.0031 0.0016 0.0005 0.0424 0.0091 0.0075 0.0038 0.0001 0.0102 0.0004 0.0241
Nucleolus 0.7788 0.8073 0.851 0.8562 0.7934 0.8246 0.7714 0.8466 0.8625 0.8106 0.7655 0.713 0.7334 0.9034 0.8836 0.8706 0.8433 0.8268
Nucleus 0.111 0.1823 0.1317 0.1306 0.1164 0.1131 0.0884 0.1254 0.0758 0.0548 0.0589 0.1355 0.0867 0.0847 0.0758 0.1037 0.1548 0.1101
Peroxisomes 0.0044 0 0 0.0001 0.0011 0.0002 0.011 0.0006 0.0033 0.0078 0.0173 0.0005 0.0122 0 0.0025 0 0 0.0001
Punctate Nuclear 0.012 0.0016 0.0005 0.0013 0.0062 0.0038 0.0101 0.0031 0.0025 0.0013 0.0066 0.0271 0.0116 0.0023 0.0003 0.0003 0.0006 0.0024
Vacuole 0.0044 0.0004 0.0003 0.0001 0.0006 0.0024 0.0022 0.0008 0.0024 0.001 0.0011 0.0013 0.0009 0.0015 0.0005 0.0027 0.0001 0.0008
Vacuole Periphery 0.0056 0.0011 0.0004 0.0004 0.0033 0.0141 0.0016 0.0009 0.0031 0.0006 0.0023 0.0236 0.0018 0.0027 0.0004 0.0113 0.0002 0.013

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 190.8805 205.6307 173.5222 119.7153 142.8251 161.3548 127.957 105.1529 84.6527 122.8105
Translational Efficiency 1.4072 1.3724 1.1843 1.4205 1.2991 1.1273 1.2408 1.2075 1.1007 1.223

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1800 1222 435 1749 1651 1924 2069 874 3451 3146 2504 2623

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 1340.04 1892.06 2348.74 2963.12 1711.85 1909.02 2391.26 2103.40 1517.92 1902.43 2383.87 2676.66
Standard Deviation 271.43 516.74 390.32 808.67 392.31 457.83 490.63 792.14 382.82 481.64 475.00 899.64
Intensity Change Log 2 — 0.497682 0.809611 1.144841 — 0.157276 0.482215 0.297167 — 0.316706 0.635184 0.731292

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000380 0.000500 0.000244 0.000911 0.000398 0.000505 0.000612 0.000957 0.000388 0.000503 0.000548 0.000927
Bud Neck 0.001242 0.001080 0.002120 0.007428 0.000951 0.001552 0.002570 0.013611 0.001103 0.001369 0.002492 0.009489
Bud Site 0.000219 0.000533 0.000615 0.001321 0.000141 0.000265 0.000355 0.004177 0.000182 0.000369 0.000400 0.002273
Cell Periphery 0.000028 0.000025 0.000012 0.000112 0.000013 0.000029 0.000019 0.000235 0.000021 0.000027 0.000018 0.000153
Cytoplasm 0.000145 0.000166 0.000024 0.000437 0.000160 0.000160 0.000045 0.007315 0.000152 0.000162 0.000042 0.002728
Cytoplasmic Foci 0.001347 0.001986 0.004799 0.006335 0.001338 0.001416 0.001570 0.011131 0.001343 0.001637 0.002131 0.007933
Eisosomes 0.000112 0.000125 0.000120 0.000205 0.000158 0.000140 0.000100 0.000118 0.000134 0.000134 0.000104 0.000176
Endoplasmic Reticulum 0.000285 0.000186 0.000078 0.000155 0.000135 0.000151 0.000100 0.000589 0.000213 0.000164 0.000096 0.000299
Endosome 0.000067 0.000057 0.000015 0.000235 0.000013 0.000095 0.000018 0.001918 0.000041 0.000080 0.000018 0.000796
Golgi 0.000114 0.000085 0.000403 0.000581 0.000082 0.000135 0.000183 0.001200 0.000099 0.000116 0.000221 0.000787
Lipid Particles 0.000198 0.000037 0.000013 0.000103 0.000015 0.000101 0.000012 0.001101 0.000110 0.000076 0.000012 0.000435
Mitochondria 0.000878 0.001061 0.000794 0.005686 0.001224 0.001057 0.002495 0.005240 0.001043 0.001059 0.002199 0.005538
Mitotic Spindle 0.001351 0.000260 0.019229 0.007819 0.000478 0.001594 0.006359 0.010109 0.000933 0.001076 0.008594 0.008582
None 0.000814 0.000744 0.000252 0.001118 0.000686 0.000507 0.000350 0.004788 0.000752 0.000599 0.000333 0.002341
Nuclear Periphery 0.000947 0.000317 0.000111 0.004408 0.000510 0.000785 0.002179 0.002033 0.000738 0.000603 0.001820 0.003617
Nuclear Periphery Foci 0.000770 0.000290 0.000104 0.002212 0.000488 0.000362 0.000834 0.001204 0.000635 0.000334 0.000707 0.001876
Nucleolus 0.634710* 0.586520* 0.703031* 0.701370* 0.646606* 0.594385* 0.700753* 0.489263* 0.640401* 0.591330* 0.701149* 0.630695*
Nucleus 0.324156* 0.372288* 0.131852* 0.214702* 0.302274* 0.364210* 0.236161* 0.403548* 0.313688* 0.367347* 0.218040* 0.277627*
Peroxisomes 0.031368 0.032117 0.135501* 0.030732 0.042921 0.030229 0.043206 0.031470 0.036895 0.030963 0.059240 0.030978
Vacuole 0.000314 0.000563 0.000307 0.003049 0.000391 0.000575 0.000319 0.005066 0.000351 0.000570 0.000317 0.003721
Vacuole Periphery 0.000556 0.001060 0.000377 0.011082 0.001021 0.001747 0.001759 0.004928 0.000778 0.001480 0.001519 0.009031

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -3.34 3.59 -12.19 -10.65 -17.70 -1.75 -8.52 -8.26 -5.12 -5.49 -2.69 -6.84 -15.07 -8.13 -10.76
Bud Neck 1.13 -5.12 -16.59 -17.18 -13.32 -3.55 -11.53 -11.50 -11.02 -10.27 -2.25 -12.13 -18.17 -17.55 -15.33
Bud Site -0.99 -8.32 -9.66 -2.95 -5.63 -2.75 -9.10 -5.67 -5.50 -5.37 -1.52 -9.55 -8.38 -7.12 -7.46
Cell Periphery 0.36 2.30 -5.60 -5.97 -6.96 -2.63 -3.33 -8.89 -8.13 -8.66 -1.25 0.77 -10.18 -9.62 -10.65
Cytoplasm -0.33 2.89 -2.85 -2.62 -3.77 0.03 1.06 -4.19 -4.19 -4.26 -0.10 1.93 -4.54 -4.52 -4.73
Cytoplasmic Foci -0.83 -6.79 -8.23 -5.47 -1.85 -0.36 -1.75 -5.56 -5.43 -5.34 -0.85 -4.53 -9.60 -8.56 -8.16
Eisosomes -1.55 -2.01 -4.92 -4.11 -4.10 2.25 8.10 4.48 2.99 -1.93 -0.09 5.75 -3.14 -3.13 -5.54
Endoplasmic Reticulum 2.37 9.59 5.93 0.77 -8.15 -0.81 4.11 -4.33 -4.08 -4.60 1.90 9.94 -2.70 -3.46 -5.48
Endosome 0.28 1.71 -1.78 -1.95 -2.47 -2.17 -3.10 -2.44 -2.32 -2.43 -1.35 1.48 -2.85 -2.68 -2.95
Golgi 1.58 -12.74 -3.51 -3.71 -1.02 -1.76 -13.57 -2.40 -2.26 -2.16 -0.91 -14.89 -3.91 -3.77 -3.13
Lipid Particles 0.93 1.06 0.56 -2.68 -4.81 -1.92 0.72 -1.79 -1.60 -1.79 0.30 1.08 -1.40 -1.70 -2.12
Mitochondria -1.82 0.37 -12.70 -12.31 -12.83 0.76 -4.89 -10.09 -11.17 -6.95 -0.17 -6.33 -15.57 -15.91 -10.61
Mitotic Spindle 2.80 -5.27 -5.13 -6.32 3.41 -1.87 -7.13 -5.00 -4.22 -1.94 -0.39 -8.25 -7.34 -6.86 0.15
None 0.34 2.78 -1.18 -1.96 -4.71 0.92 1.79 -3.32 -3.51 -3.64 1.07 3.03 -3.56 -4.11 -4.78
Nuclear Periphery 1.64 2.26 -5.28 -7.55 -8.10 -0.67 -3.32 -2.89 -1.92 0.20 0.42 -2.39 -6.45 -6.46 -3.20
Nuclear Periphery Foci 2.87 5.29 -4.81 -6.65 -7.95 0.91 -2.08 -2.23 -2.66 -1.12 2.80 -0.43 -5.58 -7.13 -5.10
Nucleolus 4.64 -5.06 -7.10 -10.79 -0.54 5.79 -6.41 13.56 9.62 18.23* 7.26 -8.99 2.95 -3.51 10.64
Nucleus -4.52 19.39 12.70 15.30 -9.24 -6.62 7.82 -8.93 -4.28 -14.52 -7.70 14.66 3.88 10.66 -9.28
Peroxisomes -0.30 -17.37 -0.20 0.13 17.39* 5.96 -0.67 4.30 -0.35 5.36 4.06 -12.07 3.48 -0.41 14.84
Vacuole -1.51 -0.34 -7.74 -6.55 -7.75 -1.16 0.72 -4.38 -4.20 -4.45 -1.93 0.49 -7.75 -7.13 -7.86
Vacuole Periphery -4.09 2.78 -11.55 -10.93 -11.78 -2.08 -2.92 -5.33 -4.05 -4.36 -3.37 -4.61 -12.48 -11.06 -11.28
Standard name
Human Ortholog
Description Component of several different pre-ribosomal particles; forms a complex with Ytm1p and Erb1p that is required for maturation of the large ribosomal subunit; required for exit from G<sub>0</sub> and the initiation of cell proliferation
Localization
Cell Percentages nucleolus (56%), nucleus (10%), mixed (32%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Nop7

Nop7


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Nop7-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available