ORF
Human Ortholog
Description Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0
Cytoplasm 0.99 0.94 0.98 0.97 0.96 0.84 0.78 0.75 0.74 0.75 0.69 0.71 1.0 0.98 0.99 0.97 0.96 0.93 0.95 0.9 0.95 0.84 0.89 0.89
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0.12 0.33 0.42 0.39 0.47 0.39 0.55 0.51 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0 0 0 0.05 0.05 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 1 0 0 1 0 0 0 0 0 0 0 0 1 1
Bud 0 0 1 0 4 7 10 13 9 7 18 17 3 1 0 0 0 0 0 0 0 0 1 3
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 2
Bud Site 0 0 0 0 0 0 0 0 1 1 1 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 1 1 0 6 2 12 1 9 6 15 9 11 9 9 7 5 14 7 0 0 0 0 0 0
Cytoplasm 88 74 119 266 333 446 409 555 400 542 275 310 211 345 334 256 255 149 91 88 117 98 257 364
Endoplasmic Reticulum 1 1 1 1 1 0 4 2 0 2 0 0 2 8 5 14 7 4 0 0 1 0 6 9
Endosome 0 0 0 1 0 3 1 7 3 8 0 2 0 1 0 1 0 0 0 1 0 1 2 0
Golgi 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 2 1 0 1 0 0
Mitochondria 1 2 3 11 43 177 222 287 255 279 217 222 0 0 0 4 6 0 1 1 0 2 2 7
Nucleus 0 3 1 1 1 2 3 10 5 3 5 2 2 0 0 3 5 2 0 0 0 0 0 2
Nuclear Periphery 0 0 0 0 0 1 4 4 0 1 2 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 1 0 3 2 0 1 1 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 1 0 2 1 1 0 0 0 0 0 0 0 0 0 0 0 1
Vac/Vac Membrane 0 0 0 2 1 4 7 20 14 20 10 6 0 0 0 1 2 12 0 2 0 6 15 13
Unique Cell Count 89 79 121 273 348 529 525 739 542 720 396 435 212 352 339 263 265 161 96 98 124 117 291 411
Labelled Cell Count 92 81 125 288 386 652 664 910 693 881 540 571 227 365 346 284 289 176 96 98 124 117 291 411


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 6.3 7.2 6.1 6.4 5.5 4.8 4.3 4.5 4.4 4.3 4.3 4.1 7.2 7.5 7.6 8.2 8.4 8.5 8.2 8.3 8.2
Std Deviation (1e-4) 0.9 1.0 0.7 1.5 1.2 1.1 1.4 1.0 1.3 1.1 1.2 1.0 0.8 1.0 1.0 1.3 1.2 1.6 1.7 1.4 1.4
Intensity Change (Log2) – – – 0.07 -0.14 -0.34 -0.49 -0.43 -0.47 -0.5 -0.51 -0.58 0.24 0.31 0.33 0.44 0.47 0.48 0.43 0.45 0.44


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm -0.6 -1.3 -4.1 -5.2 -5.8 -5.9 -5.7 -6.5 -6.3 1.1 -0.2 0.1 -0.6 -1.1 -2.2
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 3.1 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 3.1

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration – – – – – – – – – – – – – – – – – –
Actin 0.0006 0.0017 0.0004 0.0013 0 0.0002 0.0004 0.0003 0.0006 0.0014 0.0011 0.0003 – – – – – –
Bud 0.0004 0.0006 0.001 0.0009 0.0003 0.0002 0.0002 0.0004 0.0004 0.0007 0.0002 0.0002 – – – – – –
Bud Neck 0.0017 0.0004 0.0008 0.0011 0.0004 0.0008 0.0002 0.0001 0.0002 0.0003 0.0003 0.0005 – – – – – –
Bud Periphery 0.0004 0.0003 0.0009 0.0005 0.0001 0.0001 0.0002 0.0001 0.0002 0.0006 0.0002 0.0001 – – – – – –
Bud Site 0.0044 0.0031 0.0061 0.0015 0.0008 0.0001 0.0016 0.0022 0.0011 0.0005 0.0002 0 – – – – – –
Cell Periphery 0.0003 0.0001 0.0002 0.0001 0 0 0.0003 0.0003 0.0002 0.0002 0.0001 0.0001 – – – – – –
Cytoplasm 0.8687 0.9208 0.8914 0.8453 0.8869 0.8913 0.8529 0.9176 0.8458 0.7982 0.8741 0.9297 – – – – – –
Cytoplasmic Foci 0.0153 0.0193 0.0201 0.0282 0.0168 0.0183 0.0206 0.0158 0.0167 0.0209 0.0139 0.0084 – – – – – –
Eisosomes 0 0 0 0 0 0 0.0001 0 0 0 0 0 – – – – – –
Endoplasmic Reticulum 0.0089 0.0022 0.0033 0.0042 0.0019 0.0037 0.005 0.0014 0.0024 0.0047 0.0055 0.0025 – – – – – –
Endosome 0.0139 0.0139 0.0212 0.0252 0.0144 0.0201 0.0138 0.014 0.0283 0.057 0.0256 0.0078 – – – – – –
Golgi 0.0015 0.0013 0.001 0.0016 0.0004 0.0009 0.0012 0.0014 0.0014 0.0027 0.0012 0.0006 – – – – – –
Lipid Particles 0.0004 0.0001 0.0003 0.0005 0 0.0001 0.001 0.0001 0.0002 0.0007 0.0002 0.0001 – – – – – –
Mitochondria 0.0025 0.0006 0.0006 0.0009 0.0004 0.0011 0.0011 0.0002 0.0006 0.0009 0.0003 0.0003 – – – – – –
None 0.0704 0.0283 0.0379 0.0729 0.0698 0.0579 0.0901 0.0422 0.0866 0.1008 0.0708 0.0471 – – – – – –
Nuclear Periphery 0.0012 0.0004 0.001 0.0018 0.0005 0.0003 0.0009 0.0004 0.0006 0.0009 0.0006 0.0002 – – – – – –
Nucleolus 0.0001 0.0001 0.0004 0.0001 0 0 0.002 0 0.0044 0.0001 0 0 – – – – – –
Nucleus 0.0029 0.0018 0.0032 0.0029 0.0021 0.001 0.0023 0.0005 0.0009 0.0008 0.0006 0.0005 – – – – – –
Peroxisomes 0.0005 0.0005 0.0003 0.0005 0.0001 0.0002 0.0003 0.0001 0.0001 0.0001 0.0001 0 – – – – – –
Punctate Nuclear 0.0004 0.0006 0.0014 0.0024 0.0003 0.0002 0.0013 0.0001 0.0002 0.0002 0.0002 0.0001 – – – – – –
Vacuole 0.005 0.0035 0.0076 0.0067 0.0042 0.0027 0.0038 0.0024 0.0077 0.0068 0.004 0.0013 – – – – – –
Vacuole Periphery 0.0005 0.0004 0.0008 0.0014 0.0005 0.0006 0.0004 0.0004 0.0011 0.0014 0.0007 0.0003 – – – – – –

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 26.2165 15.8537 24.5512 28.2638 27.9924 21.5781 23.1733 23.7546 26.3955 30.9505
Translational Efficiency 0.6209 0.9269 0.6678 0.5709 0.5293 0.7374 0.7406 0.643 0.6951 0.536

Hit Data

Dataset Hit
Protein Concentration –
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
788 1499 1401 422 1228 921 835 1258 2016 2420 2236 1680

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 808.72 908.65 1074.50 1119.55 823.99 879.14 1093.52 1067.30 818.02 897.42 1081.60 1080.42
Standard Deviation 105.52 144.24 141.09 158.27 126.18 121.68 131.72 134.71 118.77 136.85 137.98 142.81
Intensity Change Log 2 — 0.168084 0.409953 0.469207 — 0.093466 0.408281 0.373267 — 0.130909 0.409110 0.421586

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000185 0.000367 0.000577 0.000455 0.000214 0.000688 0.000369 0.000760 0.000202 0.000489 0.000499 0.000684
Bud Neck 0.003481 0.003930 0.000973 0.001613 0.004766 0.008126 0.000725 0.002100 0.004264 0.005527 0.000880 0.001978
Bud Site 0.012555 0.021791 0.008007 0.023884 0.014194 0.034698 0.010576 0.025174 0.013554 0.026703 0.008966 0.024850
Cell Periphery 0.000231 0.000109 0.000088 0.000139 0.000195 0.000709 0.000062 0.000068 0.000209 0.000337 0.000078 0.000086
Cytoplasm 0.652316* 0.703086* 0.786140* 0.746092* 0.574708* 0.566984* 0.857927* 0.638713* 0.605043* 0.651288* 0.812948* 0.665685*
Cytoplasmic Foci 0.224168* 0.152139 0.053309 0.103178 0.259432* 0.230556* 0.059257 0.082531 0.245648* 0.181983* 0.055530 0.087717
Eisosomes 0.000058 0.000032 0.000014 0.000017 0.000087 0.000105 0.000007 0.000016 0.000075 0.000060 0.000012 0.000016
Endoplasmic Reticulum 0.002197 0.000863 0.001218 0.000436 0.004046 0.001833 0.000195 0.000258 0.003323 0.001232 0.000836 0.000303
Endosome 0.016188 0.014683 0.005503 0.004928 0.025959 0.035648 0.001880 0.012125 0.022139 0.022662 0.004150 0.010317
Golgi 0.007255 0.008222 0.000409 0.002054 0.009128 0.011662 0.000302 0.004086 0.008396 0.009531 0.000369 0.003576
Lipid Particles 0.003202 0.001319 0.001146 0.001136 0.004445 0.001927 0.000485 0.000526 0.003959 0.001551 0.000899 0.000679
Mitochondria 0.002392 0.002037 0.000056 0.000040 0.003330 0.003545 0.000033 0.002423 0.002963 0.002611 0.000047 0.001825
Mitotic Spindle 0.000285 0.002804 0.002436 0.006300 0.001244 0.002546 0.000753 0.004022 0.000869 0.002706 0.001807 0.004594
None 0.002224 0.000818 0.001366 0.000672 0.001243 0.000435 0.001485 0.000810 0.001626 0.000672 0.001411 0.000776
Nuclear Periphery 0.000083 0.000151 0.000196 0.000075 0.000271 0.000041 0.000049 0.000047 0.000197 0.000109 0.000141 0.000054
Nuclear Periphery Foci 0.000862 0.000397 0.003407 0.004019 0.000829 0.000317 0.003666 0.000649 0.000842 0.000367 0.003504 0.001495
Nucleolus 0.000244 0.000208 0.000165 0.000094 0.000369 0.000140 0.000086 0.000116 0.000320 0.000182 0.000136 0.000110
Nucleus 0.015676 0.007871 0.030500 0.009453 0.014905 0.005205 0.017076 0.005589 0.015206 0.006856 0.025487 0.006560
Peroxisomes 0.001241 0.001945 0.000457 0.001419 0.001576 0.002974 0.000307 0.001374 0.001445 0.002337 0.000401 0.001385
Vacuole 0.051198 0.073825 0.103633 0.093486 0.074001 0.086530 0.044627 0.216918* 0.065088 0.078660 0.081598 0.185913
Vacuole Periphery 0.003962 0.003402 0.000398 0.000511 0.005058 0.005330 0.000131 0.001694 0.004630 0.004136 0.000298 0.001396

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -1.11 -1.11 -4.10 -0.44 0.24 -3.25 -0.78 -1.67 0.39 -0.95 -2.48 -1.26 -2.07 -0.31 -0.40
Bud Neck -0.92 5.88 3.58 5.70 -2.58 -4.11 10.89 7.68 8.22 -7.72 -2.98 12.09 8.26 10.35 -7.65
Bud Site -5.04 2.91 -3.23 0.05 -5.35 -7.86 2.58 -2.99 4.39 -4.50 -9.21 4.66 -4.14 2.80 -6.97
Cell Periphery 6.89 8.09 6.18 -0.81 -2.89 -0.97 12.52 11.35 1.24 0.81 -0.63 13.38 13.02 1.32 1.02
Cytoplasm -4.82 -10.92 -3.56 -0.21 4.42 0.70 -27.21 -4.28 -4.57 21.08 -6.09 -25.85 -4.20 1.14 18.31
Cytoplasmic Foci 9.76 26.51 15.79 8.38 -7.77 3.69 33.13 30.83 23.53 -4.19 12.04 43.78 34.79 23.46 -9.10
Eisosomes 6.94 14.43 12.97 5.53 -1.69 -1.11 15.79 14.34 5.80 -6.47 2.26 19.25 18.18 7.21 -3.30
Endoplasmic Reticulum 3.12 2.39 4.56 3.10 3.12 2.02 5.00 4.99 2.07 -0.20 3.58 4.98 6.23 3.14 3.46
Endosome 1.05 9.30 9.35 9.47 1.26 -4.02 19.78 10.66 11.29 -8.63 -0.37 20.34 12.33 11.61 -6.16
Golgi -1.27 12.58 6.07 7.28 -1.19 -2.46 16.54 5.74 6.81 -2.78 -1.86 20.62 7.07 8.13 -2.95
Lipid Particles 4.10 4.47 4.38 0.87 -0.07 6.92 11.46 11.59 9.01 0.44 8.53 10.95 11.97 8.42 3.04
Mitochondria 0.38 3.42 3.45 2.87 1.79 -0.12 2.91 1.15 1.19 -1.98 0.37 3.94 1.72 1.44 -1.96
Mitotic Spindle -2.59 -4.61 -2.37 -1.32 -1.31 -0.93 0.46 -2.13 -0.57 -2.65 -2.16 -2.69 -3.36 -1.25 -2.00
None 3.10 2.14 3.67 1.15 3.28 2.42 -0.37 1.32 -0.39 1.84 3.53 1.23 2.69 0.26 2.24
Nuclear Periphery -0.58 -5.01 -0.46 0.44 3.92 2.73 2.33 2.43 -3.65 1.33 1.01 0.40 2.34 0.48 6.54
Nuclear Periphery Foci 4.71 -10.27 -7.74 -9.12 -0.19 5.45 -8.87 1.29 -6.70 9.43 6.80 -13.59 -5.36 -10.28 9.90
Nucleolus 0.73 1.65 4.27 3.06 2.19 3.35 4.11 3.74 1.09 -2.17 2.87 3.83 4.81 2.97 1.32
Nucleus 6.81 -10.02 3.12 -4.10 13.75 7.72 -3.43 6.41 -3.76 12.59 9.33 -10.27 7.91 -2.74 20.55
Peroxisomes -1.50 2.51 -0.48 0.77 -2.34 -2.64 4.71 1.28 3.72 -6.04 -2.58 4.97 0.69 3.33 -5.20
Vacuole -5.84 -12.21 -9.46 -7.04 -2.10 -2.76 1.32 -24.80 -21.92 -24.10 -4.62 -8.85 -26.86 -24.20 -18.75
Vacuole Periphery 1.01 9.23 8.61 6.70 -0.16 -0.36 11.70 6.67 5.77 -2.84 1.12 14.58 8.62 6.83 -2.55
ORF
Human Ortholog
Description Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm
Localization
Cell Percentages cytoplasm (98%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-4

YGR117C

YGR117C


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ygr117c-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available