Standard name
Human Ortholog
Description Essential component of the conserved oligomeric Golgi complex; a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments; the components of the Golgi complex are Gog1p through Cog8p

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0.17 0 0 0 0.07 0.07 0 0 0 0 0 0
Bud 0 0 0.05 0 0.05 0 0.06 0 0 0.08 0.05 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.07 0 0 0.05 0 0 0 0 0 0 0 0.07 0.1 0.09 0.11 0.09 0.1 0 0 0 0 0 0
Endoplasmic Reticulum 0.1 0 0 0 0 0 0 0 0 0 0 0.22 0.11 0.12 0.07 0 0.05 0 0 0 0 0 0
Endosome 0 0 0.12 0.07 0 0 0 0 0 0 0 0.1 0.25 0.2 0.4 0.36 0.37 0.07 0.07 0.15 0.12 0.14 0.12
Golgi 0.33 0 0 0 0 0 0 0 0 0 0 0.3 0.23 0.34 0.19 0.29 0.28 0.73 0.66 0.58 0.52 0.54 0.58
Mitochondria 0.81 0.94 0.88 0.92 0.95 0.94 0.91 0.98 0.97 0.95 0.96 0.32 0.29 0.25 0.16 0.07 0.05 0.09 0.18 0.12 0.23 0.2 0.17
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0.08 0.05 0 0 0 0 0 0 0 0 0 0 0.1 0.12 0 0.11 0 0.09 0.05 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.18 0.13 0.1 0 0 0 0 0 0
Vac/Vac Membrane 0.05 0 0 0 0 0 0 0 0 0 0 0.08 0.13 0.14 0.06 0.08 0.1 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 8 0 1 0 2 0 0 1 0 1 0 42 13 6 3 8 9 0 3 6 6 12 20
Bud 2 4 9 4 16 17 25 12 13 26 22 7 2 2 1 4 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 2 0 1 4 5 5 6 0 0 0 0 0 0
Cell Periphery 0 0 2 1 2 1 3 0 1 2 1 0 0 3 0 2 1 0 0 0 0 0 0
Cytoplasm 13 2 4 12 9 13 16 6 17 11 12 18 28 26 12 11 14 0 1 0 0 1 2
Endoplasmic Reticulum 19 7 2 3 6 4 13 8 5 2 7 54 32 36 7 2 7 0 1 0 2 6 3
Endosome 2 1 21 17 6 11 19 2 4 1 4 24 74 60 42 44 50 2 14 25 40 65 58
Golgi 64 5 7 0 0 0 0 1 0 1 0 76 69 102 20 35 38 23 140 100 176 248 296
Mitochondria 156 148 154 235 277 441 388 341 464 329 408 80 84 73 17 9 7 2 38 19 78 90 87
Nucleus 0 0 0 2 3 3 6 1 0 2 1 1 1 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 1 1 0 0 1 1 4 0 8 1 4 0 1 2 0 1 4 0 0 0 1 2 0
Nucleolus 1 2 1 1 3 3 4 3 7 3 5 0 0 0 2 1 3 0 0 0 0 0 0
Peroxisomes 1 2 14 14 10 12 12 3 9 5 8 0 8 2 11 15 4 3 4 15 17 14 21
SpindlePole 1 0 6 6 9 9 13 6 14 11 8 11 8 12 19 16 14 0 0 0 0 1 0
Vac/Vac Membrane 9 1 6 8 8 7 14 9 14 5 3 19 37 41 6 10 13 0 6 1 10 10 13
Unique Cell Count 192 157 175 256 293 468 426 349 479 346 424 250 294 296 105 122 135 32 212 173 340 458 510
Labelled Cell Count 277 173 227 303 354 522 518 397 561 405 489 332 358 365 140 158 164 32 212 173 340 458 510


Early Golgi, Golgi

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 6.8 5.6 5.5 5.1 4.6 4.2 4.5 4.1 3.9 4.2 4.2 7.0 6.9 7.0 6.5 6.7 6.8 8.2 7.8 7.8
Std Deviation (1e-4) 0.9 0.8 0.9 1.4 1.0 1.0 1.1 0.9 0.8 1.2 0.9 0.8 0.8 0.9 1.0 1.2 1.1 1.5 1.2 1.3
Intensity Change (Log2) -0.04 -0.13 -0.28 -0.43 -0.32 -0.45 -0.52 -0.42 -0.42 0.33 0.31 0.32 0.22 0.26 0.27 0.54 0.48 0.48


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin
Bud 1.2 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0
Cyto
Endoplasmic Reticulum 0 0 4.7 2.3 2.7 0.8 0 0.3
Endosome 4.2 2.9 3.7 6.7 5.8 8.4 8.0 8.2
Golgi 0.4 0 6.7 5.5 7.5 4.3 6.0 6.0
Mitochondria -2.0 -0.9 -12.3 -13.3 -14.1 -12.8 -14.5 -15.2
Nuclear Periphery 0 0 0 0 0 0 0 0
Nuc
Nucleolus 0 0 0 0 0 0 0 0
Peroxisomes 2.9 2.1 0 0 0 3.4 3.8 0
SpindlePole 0 0 0 0 0 5.5 4.7 4.1
Vac
Cortical Patches 0 0 5.4 0 0 0 0 0
Cytoplasm 0 1.9 2.7 3.3 3.2 3.6 3.0 3.4
Nucleus 0 0 0 0 0 0 0 0
Vacuole 0 0 3.2 4.4 4.6 0 0 3.6

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 1.6357 1.7276 1.6901 1.5829 1.4648 1.5634 2.4777 2.9885 2.8666 2.6771 2.7755 2.9291 0.2351 0.9955 0.813 0.8821 0.1242 0.9074
Actin 0.0135 0.002 0.0068 0.0028 0.0436 0.0036 0.0239 0.003 0.0159 0.0086 0.0069 0.0155 0.0112 0.0118 0.0074 0.0009 0.0006 0.0032
Bud 0.0005 0.0003 0.0004 0.0003 0.0002 0.0008 0.0005 0.0072 0.0008 0.0001 0.0005 0.0001 0.0002 0.0003 0.0003 0.0003 0.0001 0.0001
Bud Neck 0.0022 0.0003 0.0004 0.0018 0.0003 0.0009 0.0008 0.0004 0.0006 0.0002 0.0003 0.0005 0.0005 0.0004 0.0004 0.0002 0.0002 0.0003
Bud Periphery 0.0008 0.0002 0.0003 0.0002 0.0001 0.0006 0.0005 0.0029 0.0007 0.0001 0.001 0.0001 0.0002 0.0002 0.0003 0.0004 0 0.0001
Bud Site 0.0062 0.0015 0.0012 0.0011 0.0004 0.0004 0.0035 0.0069 0.0021 0.0002 0.0006 0.0002 0.0007 0.0019 0.0008 0.0002 0.0001 0.0002
Cell Periphery 0.0011 0.0001 0.0001 0.0001 0.0001 0.0002 0.0004 0.0006 0.0002 0 0.0001 0.0001 0.0001 0.0001 0.0001 0.0001 0 0.0001
Cytoplasm 0.0111 0.0174 0.0104 0.0097 0.0161 0.0071 0.0088 0.0205 0.0129 0.0181 0.0028 0.0073 0.0056 0.024 0.0133 0.0034 0.0028 0.0124
Cytoplasmic Foci 0.1519 0.1532 0.1509 0.1071 0.1547 0.1166 0.1396 0.1381 0.13 0.0944 0.1253 0.1323 0.1594 0.1471 0.1122 0.0802 0.1379 0.0945
Eisosomes 0.0006 0 0.0002 0.0001 0.0001 0 0.0011 0.0002 0.0001 0 0.0001 0.0001 0.0001 0 0.0001 0 0 0
Endoplasmic Reticulum 0.0033 0.0013 0.0011 0.0012 0.001 0.0012 0.0044 0.0014 0.0023 0.0009 0.0007 0.0018 0.0018 0.003 0.0022 0.0012 0.0016 0.0013
Endosome 0.2833 0.3552 0.3256 0.3473 0.2961 0.2544 0.3099 0.2991 0.2908 0.4209 0.3252 0.2319 0.3385 0.3655 0.3324 0.3571 0.316 0.3157
Golgi 0.3523 0.3756 0.3765 0.3555 0.343 0.4899 0.3505 0.4182 0.3911 0.4047 0.4466 0.4792 0.3217 0.3768 0.4342 0.3644 0.5119 0.5086
Lipid Particles 0.0378 0.017 0.0164 0.0132 0.0305 0.0238 0.0294 0.0049 0.022 0.0122 0.0183 0.0148 0.067 0.0109 0.0196 0.0134 0.009 0.0199
Mitochondria 0.0562 0.0447 0.0599 0.1295 0.0524 0.0492 0.0602 0.0644 0.0752 0.0097 0.0526 0.0557 0.02 0.0374 0.0448 0.1507 0.0099 0.0246
None 0.0238 0.0058 0.0029 0.0009 0.0045 0.0019 0.0059 0.0203 0.0159 0.0022 0.0003 0.016 0.0191 0.0015 0.0032 0.0002 0.0001 0.0008
Nuclear Periphery 0.0022 0.0003 0.0006 0.0008 0.001 0.0003 0.0049 0.0002 0.0006 0.0003 0.0005 0.0004 0.0008 0.0008 0.0004 0.0002 0.0006 0.0006
Nucleolus 0.0016 0.0001 0.0008 0.0002 0.0012 0.0006 0.0004 0.0009 0.0009 0 0.0001 0.0001 0.0004 0 0.0001 0 0 0.0001
Nucleus 0.001 0.0003 0.0012 0.0002 0.0015 0.0004 0.001 0.0003 0.0007 0.0001 0.0001 0.0002 0.0005 0.0002 0.0002 0.0001 0.0001 0.0002
Peroxisomes 0.0392 0.0192 0.0367 0.0174 0.0419 0.0403 0.0443 0.0067 0.0303 0.0185 0.0105 0.0407 0.044 0.0124 0.0225 0.0203 0.0048 0.0129
Punctate Nuclear 0.0044 0.0005 0.0014 0.0031 0.0039 0.002 0.0038 0.0002 0.0013 0.0002 0.0004 0.0003 0.0027 0.0003 0.0004 0.0001 0.0001 0.0003
Vacuole 0.0043 0.0029 0.0035 0.0034 0.005 0.0033 0.0034 0.0021 0.0032 0.0051 0.0036 0.0013 0.0033 0.0029 0.0026 0.0029 0.0017 0.0019
Vacuole Periphery 0.0024 0.0019 0.0027 0.0041 0.0024 0.0026 0.0029 0.0016 0.0025 0.0033 0.0034 0.0015 0.0023 0.0025 0.0027 0.0038 0.0024 0.0024

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 15.3833 13.7518 15.7704 12.25 8.1594 9.784 15.8004 10.6642 14.0279 11.7474
Translational Efficiency 0.8948 1.1224 0.9293 1.5174 1.5128 1.5452 1.0526 1.3301 1.1957 1.2259

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1784 1846 1438 1651 1324 1361 2373 1196 3108 3207 3811 2847

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 753.95 832.54 945.05 923.13 762.14 821.14 961.57 916.61 757.44 827.70 955.34 920.39
Standard Deviation 91.06 121.17 122.91 133.27 87.86 102.18 106.85 120.63 89.80 113.64 113.46 128.15
Intensity Change Log 2 0.143051 0.325922 0.292065 0.107572 0.335336 0.266252 0.125325 0.330662 0.279146

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000421 0.001586 0.002214 0.001639 0.001255 0.001373 0.003785 0.015421 0.000776 0.001496 0.003192 0.007429
Bud Neck 0.001961 0.000069 0.000522 0.001191 0.000349 0.000095 0.000206 0.000822 0.001274 0.000080 0.000326 0.001036
Bud Site 0.000627 0.000525 0.004159 0.004916 0.001355 0.000684 0.002272 0.010549 0.000937 0.000593 0.002984 0.007282
Cell Periphery 0.000093 0.000026 0.000045 0.000046 0.000085 0.000054 0.000024 0.000081 0.000090 0.000038 0.000032 0.000061
Cytoplasm 0.003015 0.000587 0.004360 0.004215 0.001833 0.000872 0.001954 0.005531 0.002511 0.000708 0.002862 0.004768
Cytoplasmic Foci 0.143579 0.145476 0.328697* 0.261829* 0.140989 0.135435 0.268206* 0.288958* 0.142475 0.141215 0.291031* 0.273226*
Eisosomes 0.000100 0.000066 0.000030 0.000028 0.000126 0.000091 0.000033 0.000133 0.000111 0.000076 0.000032 0.000072
Endoplasmic Reticulum 0.000450 0.000382 0.003699 0.000676 0.000495 0.000268 0.000691 0.000771 0.000469 0.000334 0.001826 0.000716
Endosome 0.127020 0.138560 0.138009 0.313947* 0.093057 0.131289 0.204354* 0.210215* 0.112552 0.135474 0.179320 0.270370*
Golgi 0.533027* 0.670265* 0.407230* 0.252348* 0.670039* 0.666644* 0.449743* 0.374393* 0.591394* 0.668728* 0.433701* 0.303618*
Lipid Particles 0.072336 0.005091 0.007542 0.011843 0.006655 0.006403 0.005364 0.006830 0.044356 0.005648 0.006186 0.009737
Mitochondria 0.087054 0.027363 0.005644 0.014535 0.066388 0.040725 0.011109 0.014036 0.078250 0.033033 0.009047 0.014325
Mitotic Spindle 0.001506 0.001466 0.046535 0.056050 0.000793 0.000918 0.017624 0.017216 0.001202 0.001234 0.028533 0.039736
None 0.001114 0.000020 0.000068 0.000080 0.000039 0.000113 0.000022 0.000064 0.000656 0.000059 0.000039 0.000073
Nuclear Periphery 0.000133 0.000015 0.000352 0.000678 0.000038 0.000028 0.000159 0.000045 0.000092 0.000020 0.000232 0.000412
Nuclear Periphery Foci 0.000372 0.000077 0.003032 0.003575 0.000096 0.000124 0.000987 0.000391 0.000254 0.000097 0.001758 0.002238
Nucleolus 0.000894 0.000007 0.000094 0.000471 0.000085 0.000049 0.000035 0.000100 0.000550 0.000025 0.000057 0.000315
Nucleus 0.000884 0.000019 0.000401 0.000968 0.000127 0.000030 0.000380 0.000185 0.000562 0.000024 0.000388 0.000639
Peroxisomes 0.014989 0.002678 0.003133 0.009038 0.007433 0.007266 0.003249 0.034034 0.011770 0.004625 0.003205 0.019539
Vacuole 0.004462 0.002213 0.032171 0.050294 0.002482 0.002548 0.021181 0.015254 0.003619 0.002355 0.025328 0.035574
Vacuole Periphery 0.005963 0.003510 0.012061 0.011633 0.006280 0.004992 0.008623 0.004972 0.006098 0.004139 0.009920 0.008835

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -3.57 -5.42 -2.40 -0.09 1.00 -0.35 -3.11 -7.10 -7.05 -5.48 -3.03 -4.77 -7.41 -6.49 -4.15
Bud Neck 3.40 2.54 1.21 -9.11 -4.89 2.39 1.28 -3.17 -6.50 -5.41 3.70 2.90 0.56 -11.17 -7.94
Bud Site 0.97 -9.08 -7.32 -7.48 -1.31 2.15 -2.45 -7.05 -7.71 -6.39 2.35 -8.31 -9.85 -10.52 -6.53
Cell Periphery 5.13 3.52 2.84 -2.06 -0.06 2.26 5.22 0.19 -1.87 -4.43 5.43 6.32 2.37 -2.75 -3.59
Cytoplasm 6.17 -2.03 -2.28 -5.35 -0.55 2.98 -0.49 -5.36 -6.91 -5.18 6.83 -1.18 -4.60 -8.10 -3.78
Cytoplasmic Foci -0.16 -34.75 -22.23 -23.33 11.02 1.46 -30.10 -26.67 -27.70 -3.53 0.74 -44.13 -33.86 -35.50 4.43
Eisosomes 4.90 13.67 13.91 6.37 0.61 2.40 7.38 -0.36 -2.44 -6.39 4.70 12.83 4.35 0.47 -5.83
Endoplasmic Reticulum 0.30 -5.15 -0.89 -1.25 4.72 1.61 -1.30 -1.66 -4.00 -0.50 0.97 -5.20 -1.53 -2.63 4.13
Endosome -2.43 -2.32 -27.58* -27.04* -27.41* -8.44 -25.32 -20.68 -12.85 -0.93 -6.75 -19.11 -33.63 -28.93 -19.23
Golgi -16.59 13.34 31.48* 58.61* 18.80* 0.41 28.00 32.38 33.03 8.94 -12.88 24.90 43.35* 64.35* 22.11*
Lipid Particles 15.43 14.89 13.54 -4.41 -2.87 0.14 1.12 -0.22 -0.32 -1.40 14.60 14.67 12.88 -3.78 -3.71
Mitochondria 13.22 19.96 17.16 5.35 -5.97 5.15 13.66 12.30 7.64 -1.48 13.46 23.59 21.07 9.22 -4.23
Mitotic Spindle 0.02 -12.62 -14.54 -14.50 -2.16 -0.50 -9.98 -7.16 -7.09 0.09 -0.14 -15.82 -16.01 -15.94 -4.08
None 2.05 1.96 1.93 -3.17 -0.54 -0.82 2.40 -0.75 0.48 -1.19 1.94 2.01 1.89 -0.45 -1.70
Nuclear Periphery 2.47 -3.84 -3.97 -5.09 -2.47 0.52 -5.12 -0.50 -1.53 6.02 2.52 -4.33 -4.00 -5.16 -2.38
Nuclear Periphery Foci 5.24 -6.45 -5.12 -5.59 -1.09 -0.69 -5.78 -1.96 -1.82 1.71 4.39 -8.11 -5.42 -5.84 -1.63
Nucleolus 2.96 2.66 1.40 -5.01 -3.83 0.46 0.72 -0.39 -1.02 -1.42 2.99 2.81 1.29 -4.90 -4.39
Nucleus 3.81 1.65 -0.31 -1.99 -1.14 1.38 -1.10 -0.84 -1.99 0.70 4.02 0.63 -0.44 -2.23 -0.81
Peroxisomes 11.21 10.17 4.23 -6.72 -5.75 0.12 4.33 -8.85 -8.65 -10.45 8.37 10.74 -5.09 -10.43 -11.69
Vacuole 4.50 -13.21 -18.67 -19.77 -6.01 -0.17 -15.88 -9.11 -9.17 3.00 3.81 -19.90 -20.30 -21.30 -5.93
Vacuole Periphery 4.72 -6.01 -6.43 -9.93 0.43 1.70 -2.85 1.35 0.03 3.82 4.45 -6.29 -4.11 -7.65 1.54
Standard name
Human Ortholog
Description Essential component of the conserved oligomeric Golgi complex; a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments; the components of the Golgi complex are Gog1p through Cog8p
Localization
Cell Percentages Golgi (15%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Cog2

Cog2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Cog2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available