Standard name
Human Ortholog
Description Ammonium permease; belongs to Mep-Amt-Rh family of well-conserved ammonium (NH4+) transporters that includes the human Rh factors; expression is under the nitrogen catabolite repression regulation; activity regulated by TORC1 effectors, Npr1p and Par32p; human homolog RHCG complements yeast null mutant; mutations in human homolog RHCG implicated in metabolic acidosis; MEP1 has a paralog, MEP3, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.27 0.26 0.19 0.38 0.33 0.44 0.31 0.2 0.15 0.15 0.12 0.08 0.3 0.25 0.27 0.16 0.07 0.07 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0.07 0.08 0.06 0.07 0.09 0.08 0 0 0 0 0.06 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0.89 0.92 0.91 0.94 0.81 0.74 0.63 0.4 0.5 0.4 0.39 0.37 0.94 0.89 0.84 0.65 0.68 0.81 0.92 0.91 0.92 0.89 0.88 0.88
Cytoplasm 0 0.05 0.05 0 0.08 0.18 0.24 0.3 0.4 0.46 0.56 0.61 0.05 0.1 0.14 0.34 0.33 0.15 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0.14 0.13 0.2 0.25 0.22 0.19 0.14 0.14 0 0 0 0.08 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.09 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.11 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0.06 0 0.05 0 0.07 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 51 104 68 101 74 170 166 84 58 60 49 37 42 29 32 14 5 5 4 12 8 4 9 8
Bud 2 6 4 5 6 17 35 34 25 26 38 37 3 1 1 3 4 0 1 6 3 2 5 5
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 2 3
Bud Site 0 1 0 0 0 0 0 1 2 1 1 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 170 368 325 250 180 284 335 167 199 158 160 164 130 102 100 57 49 59 245 455 383 138 238 267
Cytoplasm 7 18 18 11 17 69 128 126 158 182 228 274 7 12 17 30 24 11 1 5 2 0 2 2
Endoplasmic Reticulum 3 7 10 3 30 51 107 105 88 77 58 61 2 2 2 7 0 0 2 6 5 3 3 6
Endosome 0 0 0 0 0 0 0 1 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 1 0 3 0 0 0 0 0 0 0 0 2 0 0 0 0 0 1 0 0 0 0 0 0
Mitochondria 17 5 7 4 0 5 1 4 0 1 2 2 0 0 0 3 3 8 6 6 8 1 2 4
Nucleus 0 0 0 0 2 0 2 2 2 6 4 5 0 0 0 1 0 1 0 0 0 0 0 0
Nuclear Periphery 1 0 1 0 0 1 2 5 1 0 0 0 0 0 0 0 0 0 0 1 1 0 0 0
Nucleolus 0 0 0 0 0 1 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 2 1 2 1 0 0 2 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 4 3 1 0 1 0 1 0 2 1 1 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 1 0 0 0 1 4 25 7 18 18 31 1 0 0 0 0 0 1 1 0 0 1 2
Unique Cell Count 190 398 359 267 221 383 534 416 395 395 410 447 139 115 119 88 72 73 266 499 418 155 270 305
Labelled Cell Count 252 510 436 374 309 605 784 557 541 531 558 617 185 148 153 116 85 85 266 499 418 155 270 305


Cell Periphery

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 28.5 28.6 25.4 26.0 23.6 20.6 20.3 18.9 19.1 18.5 18.4 18.2 22.9 19.9 18.8 19.1 24.1 29.7 22.7 24.1 27.3
Std Deviation (1e-4) 6.5 6.7 6.7 6.5 6.9 7.2 6.5 6.7 5.9 6.0 5.7 5.4 5.9 6.0 5.8 6.0 7.9 9.3 7.3 7.2 7.6
Intensity Change (Log2) – – – 0.03 -0.1 -0.3 -0.32 -0.42 -0.41 -0.46 -0.47 -0.48 -0.15 -0.35 -0.43 -0.41 -0.08 0.23 -0.16 -0.07 0.1


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 5.3 4.0 7.4 4.0 0.4 -1.6 -1.4 -2.7 -4.5 2.7 1.5 1.8 -0.7 -2.5 -2.5
Bud 0 0 2.7 3.9 4.5 3.7 3.8 5.0 4.6 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 1.4 -3.2 -5.8 -9.3 -14.5 -12.0 -14.4 -14.8 -15.6 1.1 -0.6 -2.0 -6.1 -5.1 -2.4
Cytoplasm -0.5 1.3 5.5 7.5 9.0 11.3 12.8 15.0 16.5 0 2.1 3.4 7.9 7.4 0
Endoplasmic Reticulum -1.4 5.0 5.2 7.5 8.8 7.9 7.2 5.5 5.4 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 -0.7 0 -1.2 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 4.7 0 4.1 4.0 5.1 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 4.5208 7.0007 5.6957 6.3225 5.8545 7.0587 6.7608 10.7437 8.1218 7.9164 8.4091 9.7054 7.176 11.2967 9.111 7.6007 8.791 9.4971
Actin 0.0509 0.0141 0.0154 0.0132 0.0161 0.008 0.0218 0.0026 0.0157 0.0174 0.0163 0.0077 0.0129 0.0111 0.008 0.0059 0.0024 0.0087
Bud 0.0044 0.0073 0.0088 0.0337 0.008 0.0022 0.0016 0.0033 0.0027 0.0062 0.0019 0.0008 0.002 0.0024 0.0023 0.0038 0.0007 0.0012
Bud Neck 0.0093 0.0036 0.0072 0.0366 0.0039 0.0043 0.0046 0.0022 0.0059 0.0059 0.0068 0.0102 0.0018 0.0042 0.0066 0.0081 0.0029 0.013
Bud Periphery 0.0448 0.0305 0.055 0.0341 0.076 0.0349 0.0179 0.0286 0.0351 0.0746 0.0127 0.0209 0.025 0.0155 0.0216 0.0339 0.0152 0.0231
Bud Site 0.0333 0.12 0.0599 0.0179 0.0345 0.003 0.0294 0.0756 0.0334 0.0244 0.0325 0.0018 0.0202 0.0989 0.0424 0.0118 0.0227 0.003
Cell Periphery 0.4806 0.4887 0.5428 0.6021 0.4944 0.6735 0.7217 0.7462 0.7294 0.7725 0.7554 0.8313 0.7742 0.7075 0.7812 0.8136 0.844 0.8303
Cytoplasm 0.2067 0.2601 0.1821 0.0883 0.1691 0.166 0.0831 0.1049 0.126 0.0475 0.092 0.083 0.0838 0.1058 0.0875 0.0649 0.0767 0.0732
Cytoplasmic Foci 0.0106 0.0043 0.0124 0.0017 0.0086 0.0032 0.002 0.0006 0.0007 0.0006 0.0043 0.0008 0.0011 0.001 0.0009 0.005 0.0002 0.0005
Eisosomes 0.0228 0.0087 0.0091 0.0093 0.0019 0.0069 0.0361 0.0048 0.0166 0.0174 0.0187 0.0112 0.0226 0.0169 0.0126 0.0077 0.0065 0.0068
Endoplasmic Reticulum 0.0249 0.0105 0.0117 0.0682 0.0056 0.0164 0.0172 0.0097 0.0067 0.019 0.0124 0.0152 0.0114 0.0154 0.0107 0.0149 0.0183 0.015
Endosome 0.0233 0.0028 0.017 0.0023 0.0271 0.003 0.0018 0.0001 0.0002 0.0004 0.0118 0.0012 0.0004 0.0004 0.0001 0.0005 0 0.0002
Golgi 0.0122 0.0014 0.0082 0.0018 0.018 0.0017 0.0018 0.0001 0.0009 0.0018 0.0073 0.0008 0.0014 0.0009 0.0007 0.0004 0.0004 0.0008
Lipid Particles 0.0136 0.002 0.0073 0.0057 0.0056 0.0076 0.0035 0.0006 0.0009 0.0022 0.0018 0.0017 0.0017 0.0013 0.0012 0.0009 0.0013 0.0026
Mitochondria 0.0136 0.0013 0.0109 0.0505 0.0191 0.0068 0.0028 0.0005 0.0007 0.0007 0.002 0.0042 0.0015 0.0005 0.0002 0.0008 0.0001 0.0025
None 0.0171 0.0358 0.033 0.0185 0.0576 0.0397 0.0431 0.014 0.0217 0.0016 0.017 0.0027 0.0314 0.0122 0.0197 0.0195 0.0007 0.0125
Nuclear Periphery 0.0067 0.0009 0.0011 0.0018 0.0006 0.0016 0.0019 0.0009 0.0006 0.001 0.0005 0.0012 0.0011 0.0012 0.0005 0.0008 0.0006 0.0005
Nucleolus 0.0009 0.0003 0.0004 0.0013 0.0003 0.0002 0.0004 0.0002 0.0001 0.0002 0.0003 0.0001 0.0002 0.0001 0.0001 0.0001 0.0001 0.0001
Nucleus 0.0037 0.0007 0.0011 0.002 0.0008 0.0058 0.0007 0.0003 0.0002 0.0004 0.0005 0.0002 0.0003 0.0003 0.0002 0.0003 0.0002 0.0001
Peroxisomes 0.0017 0.0003 0.0065 0.001 0.0018 0.0025 0.0012 0 0.0001 0.0001 0.0003 0.0001 0.0003 0.0001 0.0001 0.0017 0 0.0014
Punctate Nuclear 0.0023 0.0002 0.0009 0.0004 0.0001 0.0008 0.0004 0.0001 0.0001 0.0001 0.0001 0.0003 0.0001 0.0001 0.0001 0.0002 0 0
Vacuole 0.0148 0.0063 0.0085 0.0085 0.0484 0.0113 0.0068 0.0046 0.0022 0.006 0.0051 0.0045 0.0066 0.0041 0.0033 0.005 0.0069 0.0042
Vacuole Periphery 0.0015 0.0002 0.0009 0.0008 0.0026 0.0003 0.0003 0.0001 0 0.0002 0.0003 0.0001 0.0002 0.0001 0 0.0001 0.0001 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 8.2065 4.0015 8.9079 8.1771 6.771 6.7107 10.9578 6.8268 9.2652 8.6863
Translational Efficiency 0.9731 1.0696 0.4253 0.6026 0.674 0.8996 0.4354 0.5778 0.6701 0.4936

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
111 366 335 1319 2349 1891 2368 1682 2460 2257 2703 3001

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 1246.37 1867.64 1439.43 865.89 1925.47 1754.03 1616.60 844.35 1894.83 1772.45 1594.64 853.82
Standard Deviation 244.96 433.49 224.55 125.83 415.92 389.89 310.83 120.58 433.31 399.48 307.08 123.38
Intensity Change Log 2 — 0.583484 0.207765 -0.525477 — -0.134537 -0.252248 -1.189298 — 0.191335 -0.053661 -0.891121

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.013369 0.002710 0.041324 0.031179 0.000233 0.001744 0.006265 0.027360 0.000826 0.001901 0.010610 0.029039
Bud Neck 0.023834 0.002637 0.016707 0.027943 0.000478 0.001269 0.005010 0.027754 0.001532 0.001491 0.006460 0.027837
Bud Site 0.044634 0.030657 0.176814* 0.080270 0.002269 0.022945 0.037744 0.079615 0.004180 0.024195 0.054980 0.079903
Cell Periphery 0.574592* 0.931156* 0.616028* 0.035043 0.982616* 0.951600* 0.914205* 0.034186 0.964205* 0.948285* 0.877250* 0.034563
Cytoplasm 0.068602 0.007699 0.061414 0.389064* 0.000723 0.006720 0.016705 0.434047* 0.003786 0.006879 0.022246 0.414276*
Cytoplasmic Foci 0.008021 0.003548 0.007410 0.032005 0.000085 0.000875 0.000331 0.036855 0.000443 0.001309 0.001209 0.034723
Eisosomes 0.005410 0.004940 0.009116 0.020624 0.001157 0.001946 0.004516 0.016701 0.001349 0.002431 0.005086 0.018425
Endoplasmic Reticulum 0.007197 0.002825 0.021737 0.007232 0.001639 0.004344 0.004781 0.005018 0.001890 0.004098 0.006883 0.005991
Endosome 0.008304 0.001468 0.004136 0.001961 0.000313 0.000643 0.000691 0.003579 0.000673 0.000777 0.001118 0.002868
Golgi 0.007897 0.002376 0.005042 0.001044 0.000329 0.000616 0.000467 0.001663 0.000670 0.000902 0.001034 0.001391
Lipid Particles 0.002522 0.001034 0.014124 0.003493 0.000126 0.000422 0.000986 0.003556 0.000234 0.000521 0.002614 0.003528
Mitochondria 0.018777 0.000236 0.000251 0.000569 0.000224 0.000957 0.000546 0.001419 0.001061 0.000840 0.000509 0.001045
Mitotic Spindle 0.002520 0.000548 0.000773 0.004291 0.000425 0.000547 0.000488 0.003365 0.000519 0.000547 0.000523 0.003772
None 0.000034 0.000019 0.000045 0.003650 0.000015 0.000228 0.000058 0.001912 0.000016 0.000194 0.000056 0.002676
Nuclear Periphery 0.000226 0.000292 0.000290 0.000268 0.001105 0.000755 0.000675 0.000286 0.001065 0.000680 0.000627 0.000278
Nuclear Periphery Foci 0.001257 0.000158 0.001845 0.001695 0.000101 0.000115 0.000173 0.001105 0.000153 0.000122 0.000380 0.001364
Nucleolus 0.000018 0.000024 0.000017 0.000208 0.000039 0.000027 0.000031 0.000356 0.000038 0.000027 0.000029 0.000291
Nucleus 0.006394 0.000288 0.001648 0.154031* 0.000086 0.000322 0.000802 0.150513* 0.000371 0.000317 0.000907 0.152059*
Peroxisomes 0.000510 0.000104 0.000910 0.001517 0.000074 0.000179 0.000386 0.001690 0.000093 0.000167 0.000451 0.001614
Vacuole 0.196084 0.006581 0.020068 0.203786* 0.006584 0.003024 0.004679 0.167841 0.015135 0.003601 0.006586 0.183639
Vacuole Periphery 0.009796 0.000699 0.000301 0.000125 0.001380 0.000721 0.000462 0.001180 0.001759 0.000718 0.000442 0.000716

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 2.67 -4.68 -2.44 -8.95 3.36 -4.96 -9.89 -11.83 -10.96 -8.33 -3.22 -11.73 -15.29 -14.46 -7.67
Bud Neck 2.71 0.84 0.10 -11.89 -2.59 -5.09 -12.65 -15.59 -15.07 -12.37 0.05 -9.66 -18.73 -19.61 -14.54
Bud Site 1.19 -7.36 -1.33 -4.65 8.52 -7.92 -13.86 -17.37 -10.36 -7.08 -8.00 -17.29 -21.43 -11.97 -3.19
Cell Periphery -8.93 -1.33 12.86 90.12* 31.40* 7.88 16.85 339.04* 212.82* 196.03* 3.29 16.20 274.55 240.20* 176.55*
Cytoplasm 5.79 0.69 -22.72 -45.94 -30.69 -5.93 -11.12 -64.69* -63.09* -60.75* -2.76 -11.39 -79.47* -78.12* -72.80*
Cytoplasmic Foci 1.48 0.26 -7.32 -12.18 -11.33 -2.32 -6.00 -23.18 -22.17 -23.02 -2.02 -3.31 -30.61 -28.22 -29.67
Eisosomes 0.17 -2.36 -4.76 -5.39 -3.43 -3.67 -9.89 -11.48 -10.71 -8.25 -4.06 -11.46 -14.19 -12.76 -9.79
Endoplasmic Reticulum 1.95 -4.58 0.26 -3.31 5.93 -3.72 -5.43 -6.67 -0.66 -0.09 -3.40 -8.06 -7.74 -2.22 1.96
Endosome 2.11 1.23 2.01 -0.66 3.20 -3.78 -4.70 -7.67 -6.77 -6.68 -0.60 -2.51 -6.98 -7.07 -5.81
Golgi 1.47 0.76 2.03 1.24 6.33 -3.79 -1.53 -2.26 -1.65 -1.95 -0.93 -1.98 -1.37 -0.62 -0.31
Lipid Particles 1.53 -3.63 -1.08 -2.52 3.46 -2.38 -5.71 -15.24 -12.34 -9.56 -1.68 -5.66 -18.14 -12.07 -1.67
Mitochondria 1.77 1.77 1.74 -1.72 -1.65 -2.70 -5.64 -3.27 -0.72 -2.24 0.37 1.13 0.23 -0.25 -2.15
Mitotic Spindle 1.46 1.29 -0.95 -2.78 -2.61 -1.95 -2.21 -3.72 -3.54 -3.64 -0.42 -0.11 -4.38 -4.34 -4.38
None 1.27 -0.88 -4.22 -4.25 -4.21 -1.62 -2.67 -3.44 -2.91 -3.36 -1.62 -2.87 -5.46 -4.87 -5.37
Nuclear Periphery -0.92 -0.97 -3.43 -3.07 -3.70 4.31 5.66 10.19 8.79 8.98 5.09 6.08 9.69 7.49 7.70
Nuclear Periphery Foci 5.49 -1.47 -2.20 -12.85 0.08 -0.49 -2.63 -12.65 -12.98 -12.27 1.32 -4.56 -17.12 -18.50 -13.02
Nucleolus -0.54 0.21 -3.46 -3.41 -3.50 5.47 4.09 -1.55 -1.62 -1.60 5.54 4.37 -2.27 -2.39 -2.37
Nucleus 1.66 1.34 -23.05 -31.38 -31.08 -2.59 -6.39 -35.18 -35.12 -34.99* 0.52 -2.37 -46.98 -47.08 -46.87
Peroxisomes 3.16 -1.81 -3.41 -5.89 -1.44 -3.18 -3.15 -7.05 -6.59 -5.18 -2.35 -3.94 -9.10 -8.68 -5.92
Vacuole 6.94 6.43 -1.65 -34.35 -30.32 4.79 2.42 -35.38 -36.33 -35.99 6.98 5.11 -44.47 -50.38 -49.52
Vacuole Periphery 2.01 2.10 2.15 5.82 4.34 2.38 3.35 0.86 -0.80 -1.54 3.11 3.97 2.97 0.53 -0.84
Protein Complexes26S Proteasome complex37S mitochondrial small ribosomal subunit54S mitochondrial large ribosomal subunit60S cytosolic large ribosomal subunitAar2-U5 snRNPAnaphase-Promoting Complex variant 1Anaphase-Promoting Complex variant 2Anaphase-Promoting Complex variant 3Anaphase-Promoting Complex variant 4Arp2/3 complexATG1 kinase complexBLOC-1 complexCcr4-Not complexCentral kinetochore CTF19 complexCleavage and polyadenylation specificity factorcomplexCMG helicase complexCMG-Pol epsilon complexCOMPASS complexCOP9 signalosome COPI vesicle coat complexCoQ6 biosynthesis complexCore mediator complexCORVET complexCTF18-RFC complexCURI complex variant 1CURI complex variant 2CURI complex variant 3Cytoplasmic exosome complex, DIS3 variantDASH complexDNA-directed RNA Polymerase I complexDNA-directed RNA polymerase II complexDNA-directed RNA polymerase III complexDNA replication factor C complexEGO complexELG1-RFC complex Elongator holoenzyme complexEndoplasmic Reticulum Membrane ComplexEukaryotic translation initiation factor 2BcomplexEukaryotic translation initiation factor 3core complexExocystExomer complexFAR complexGeneral transcription factor complex TFIIDGeneral transcription factor complex TFIIHGET4-GET5 transmembrane domain recognition complexGID ubiquitin ligase complexGin4 complexGolgi transport complexGSE complexHMC complexHOPS complexINO80 chromatin remodeling complexLSM1-7 complexLsm1-7-Pat1 complexLSM2-7 complexLSM2-8 complexMCM complexMICOS complexMitochondrial electron transport complex IIIMitochondrial inner membrane pre-sequence translocasecomplexMitochondrial outer membrane translocase holocomplexMitochondrial proton-transporting ATP synthase complexMitochondrial pyruvate dehydrogenase complexNineTeen complexNOP8 60s ribosome pre-assembly complexNuA3 histone acetyltransferase complexNuA4 histone acetyltransferase complexNuclear condensin complexNuclear/nucleolar exosome complex, DIS3-RRP6 variantNuclear pore complexNucleolar ribonuclease MRP complexNucleolar ribonuclease P complexOligosaccharyl transferase complex variant 1Oligosaccharyl transferase complex variant 2PAF1 complexPAS complexPhosphatidylinositol 3-kinase complex IPolarisomePrefoldin complexPRP19-associated complexRad17 RFC-like complexRetromer complexRPD3L histone deacetylase complexRPD3S histone deacetylase complexRQC complexRSC complex variant RSC1RSC complex variant RSC2SAGA complexSCF-Met30 ubiquitin ligase complexSCF-Ufo1 ubiquitin ligase complexSEA complexSeptin complexSET3C histone deacetylase complexSignal recognition particleSLIK (SAGA-like) complexSmall ribosomal subunit processome, variant1Small ribosomal subunit processome, variant2Small ribosomal subunit processome, variant3SMC5-SMC6 SUMO ligase complexSm complexSpliceosomal commitment complexSPOTS complexSWI/SNF chromatin remodelling complexSwr1 chromatin remodelling complexTHO complexTranscription factor TFIIIC complexTranslocon complexTRAPPIII protein complexTRAPPII protein complexTRAPPI protein complexTREX complexU1 snRNPU2 snRNPU4 snRNPU4/U6 snRNPU4/U6.U5 tri-snRNP complexU5 snRNPU6 snRNP complexUTP-A complexUTP-B complexUTP-C complex variant 1UTP-C complex variant 2UTP-C complex variant 3Vacuolar proton translocating ATPase complex,Golgi variantVacuolar proton translocating ATPase complex,vacuole variantCellular Components••••••••••••••••actinbud neckcell peripherycytoplasmendosomeERGolgimitochondrionmixednuclear peripherynucleolusnucleusperoxisomespindle polevacuolar membranevacuole
Standard name
Human Ortholog
Description Ammonium permease; belongs to Mep-Amt-Rh family of well-conserved ammonium (NH4+) transporters that includes the human Rh factors; expression is under the nitrogen catabolite repression regulation; activity regulated by TORC1 effectors, Npr1p and Par32p; human homolog RHCG complements yeast null mutant; mutations in human homolog RHCG implicated in metabolic acidosis; MEP1 has a paralog, MEP3, that arose from the whole genome duplication
Localization
Cell Percentages cell periphery (96%)
Cell Cycle Regulation No
Subcompartmental Group cell periphery-1

Mep1

Mep1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Mep1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available