ORF | |
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Human Ortholog | |
Description | Protein that may be involved in pH regulation; probable ortholog of A. nidulans PalC, which is involved in pH regulation and binds to the ESCRT-III complex; null mutant does not properly process Rim101p and has decreased resistance to rapamycin; GFP-fusion protein is cytoplasmic; relative distribution to cytoplasm increases upon DNA replication stress |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 4 | 10 | 8 | 2 | 7 | 4 | 4 | 2 | 3 | 0 | 0 | 0 | 11 | 19 | 20 | – | – | – | 33 | 34 | 87 | 27 | 70 | 77 |
Bud | 2 | 10 | 14 | 7 | 12 | 11 | 28 | 21 | 18 | 17 | 10 | 15 | 5 | 10 | 5 | – | – | – | 0 | 0 | 0 | 0 | 0 | 3 |
Bud Neck | 0 | 0 | 1 | 0 | 1 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 2 | 2 | – | – | – | 1 | 8 | 5 | 3 | 10 | 11 |
Bud Site | 1 | 0 | 0 | 0 | 1 | 3 | 2 | 3 | 0 | 5 | 1 | 2 | 0 | 0 | 0 | – | – | – | – | – | – | – | – | – |
Cell Periphery | 0 | 1 | 12 | 10 | 6 | 3 | 11 | 7 | 6 | 8 | 4 | 4 | 3 | 8 | 9 | – | – | – | 1 | 0 | 3 | 2 | 3 | 3 |
Cytoplasm | 87 | 107 | 151 | 94 | 172 | 173 | 233 | 205 | 160 | 168 | 89 | 80 | 149 | 215 | 298 | – | – | – | 58 | 66 | 78 | 78 | 127 | 161 |
Endoplasmic Reticulum | 1 | 5 | 1 | 1 | 4 | 0 | 3 | 2 | 2 | 1 | 1 | 1 | 7 | 4 | 2 | – | – | – | 2 | 0 | 2 | 0 | 0 | 0 |
Endosome | 0 | 0 | 16 | 4 | 6 | 5 | 2 | 21 | 2 | 1 | 4 | 2 | 1 | 1 | 1 | – | – | – | 7 | 7 | 11 | 9 | 11 | 9 |
Golgi | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | 0 | 0 | 1 | 0 | 1 | 0 |
Mitochondria | 13 | 2 | 7 | 18 | 13 | 61 | 110 | 104 | 127 | 134 | 100 | 92 | 0 | 0 | 0 | – | – | – | 4 | 8 | 21 | 5 | 13 | 9 |
Nucleus | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 1 | 3 | 4 | 2 | 1 | 0 | 0 | 1 | – | – | – | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 2 | 0 | 1 | 0 | 1 | 3 | 2 | 0 | 1 | 1 | 0 | 0 | 0 | – | – | – | 0 | 0 | 1 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 12 | 3 | 2 | 1 | 0 | 6 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | 1 | 0 | 4 | 1 | 1 | 1 |
SpindlePole | 1 | 0 | 15 | 9 | 8 | 5 | 6 | 9 | 4 | 1 | 2 | 3 | 5 | 3 | 8 | – | – | – | 1 | 0 | 4 | 0 | 3 | 1 |
Vac/Vac Membrane | 0 | 0 | 7 | 1 | 4 | 3 | 10 | 22 | 20 | 24 | 13 | 8 | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 | 3 | 3 | 2 |
Unique Cell Count | 98 | 122 | 204 | 114 | 207 | 226 | 342 | 320 | 282 | 281 | 170 | 169 | 167 | 237 | 326 | 113 | 131 | 227 | 135 | 251 | 286 | |||
Labelled Cell Count | 110 | 135 | 246 | 150 | 238 | 269 | 412 | 407 | 348 | 364 | 227 | 210 | 181 | 262 | 346 | 113 | 131 | 227 | 135 | 251 | 286 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 4.6 | 5.8 | 6.3 | 6.2 | 6.5 | 5.4 | 5.3 | 5.3 | 4.8 | 4.9 | 4.3 | 4.6 | 6.3 | 6.5 | 6.3 | – | – | – | 5.7 | 6.6 | 7.0 |
Std Deviation (1e-4) | 0.5 | 0.9 | 2.6 | 3.1 | 3.8 | 2.4 | 2.1 | 1.8 | 1.1 | 2.1 | 1.3 | 1.7 | 1.0 | 1.4 | 1.1 | – | – | – | 0.9 | 1.4 | 1.6 |
Intensity Change (Log2) | – | – | – | -0.03 | 0.04 | -0.24 | -0.26 | -0.26 | -0.39 | -0.36 | -0.57 | -0.45 | -0.01 | 0.03 | -0.0 | – | – | – | -0.15 | 0.05 | 0.14 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | -0.3 | -1.4 | 0 | 0 | 0 | 0 | 0 | 0 | 1.2 | 1.8 | 1.1 | – | – | – |
Bud | -0.2 | -0.4 | -0.9 | 0.6 | -0.1 | 0 | -0.4 | 0 | 0 | -1.7 | -1.2 | -3.2 | – | – | – |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – |
Cell Periphery | 1.0 | -1.5 | -2.6 | -1.5 | -2.2 | 0 | -1.7 | 0 | 0 | -2.0 | -1.3 | -1.8 | – | – | – |
Cytoplasm | 1.7 | 2.2 | 0.6 | -1.5 | -2.4 | -3.9 | -3.3 | -4.4 | -5.3 | 3.7 | 4.7 | 5.4 | – | – | – |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – |
Endosome | -1.5 | -2.2 | -2.7 | -4.6 | -0.6 | 0 | -4.4 | 0 | 0 | -3.3 | -4.0 | -4.8 | – | – | – |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – |
Mitochondria | 3.9 | 1.3 | 6.7 | 7.9 | 7.9 | 0 | 10.6 | 0 | 0 | 0 | 0 | 0 | – | – | – |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – |
Peroxisomes | -1.3 | -2.7 | -3.3 | 0 | -2.5 | 0 | -4.1 | 0 | 0 | -3.2 | -3.8 | 0 | – | – | – |
SpindlePole | 0.2 | -1.5 | -2.5 | -3.3 | -2.4 | 0 | -4.3 | 0 | 0 | -1.8 | -3.2 | -2.7 | – | – | – |
Vacuole | 0 | -0.9 | 0 | -0.3 | 1.7 | 1.7 | 2.3 | 1.8 | 0.6 | 0 | 0 | 0 | – | – | – |
R1 | R2 | R3 | ||||||||||||||||
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G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 1.416 | 1.7199 | 1.5974 | 1.2043 | 1.3516 | 1.6842 | 0.8388 | 1.2548 | 1.147 | 1.064 | 0.8837 | 0.9534 | 0.062 | 0.4629 | -0.0727 | 0.1903 | 0.032 | 0.1783 |
Actin | 0.0574 | 0.0343 | 0.0444 | 0.0979 | 0.0719 | 0.0257 | 0.0582 | 0.0191 | 0.0625 | 0.0628 | 0.0357 | 0.0384 | 0.0451 | 0.0413 | 0.0932 | 0.0196 | 0.0043 | 0.0254 |
Bud | 0.0008 | 0.0006 | 0.0005 | 0.0013 | 0.0005 | 0.0003 | 0.0012 | 0.0017 | 0.0012 | 0.0016 | 0.0032 | 0.0018 | 0.0003 | 0.0005 | 0.002 | 0.0005 | 0.0001 | 0.0003 |
Bud Neck | 0.0046 | 0.0022 | 0.0051 | 0.0029 | 0.013 | 0.0078 | 0.0041 | 0.0015 | 0.0029 | 0.0018 | 0.0059 | 0.01 | 0.0023 | 0.0011 | 0.0039 | 0.0012 | 0.0004 | 0.0195 |
Bud Periphery | 0.0009 | 0.0009 | 0.0004 | 0.0022 | 0.001 | 0.0004 | 0.0016 | 0.001 | 0.0012 | 0.0014 | 0.0049 | 0.0026 | 0.0003 | 0.0003 | 0.0016 | 0.0006 | 0 | 0.0002 |
Bud Site | 0.0074 | 0.0085 | 0.0015 | 0.001 | 0.0005 | 0.0002 | 0.0047 | 0.02 | 0.0023 | 0.0037 | 0.0128 | 0.0012 | 0.0024 | 0.0143 | 0.0144 | 0.0007 | 0.0001 | 0.0021 |
Cell Periphery | 0.0008 | 0.0005 | 0.0005 | 0.0003 | 0.0002 | 0.0002 | 0.0005 | 0.0004 | 0.0003 | 0.0001 | 0.0004 | 0.0001 | 0.0003 | 0.0007 | 0.0004 | 0.0002 | 0.0001 | 0.0002 |
Cytoplasm | 0.4353 | 0.496 | 0.4415 | 0.5856 | 0.4289 | 0.5665 | 0.5767 | 0.7521 | 0.6268 | 0.6982 | 0.5972 | 0.7868 | 0.4913 | 0.7041 | 0.5348 | 0.6523 | 0.5711 | 0.6835 |
Cytoplasmic Foci | 0.128 | 0.0839 | 0.1123 | 0.0722 | 0.1404 | 0.0635 | 0.0896 | 0.039 | 0.0751 | 0.0742 | 0.0482 | 0.0578 | 0.1043 | 0.0309 | 0.0972 | 0.0652 | 0.022 | 0.0733 |
Eisosomes | 0.0004 | 0.0003 | 0.0003 | 0.0002 | 0.0003 | 0.0002 | 0.0004 | 0.0001 | 0.0002 | 0.0001 | 0.0003 | 0.0001 | 0.0002 | 0.0002 | 0.0002 | 0.0002 | 0.0001 | 0.0001 |
Endoplasmic Reticulum | 0.0037 | 0.0021 | 0.0031 | 0.0059 | 0.0027 | 0.0029 | 0.0103 | 0.0051 | 0.0054 | 0.0043 | 0.0063 | 0.006 | 0.0074 | 0.0044 | 0.0039 | 0.0071 | 0.0059 | 0.0074 |
Endosome | 0.0077 | 0.0063 | 0.0074 | 0.0128 | 0.0063 | 0.0041 | 0.0245 | 0.0057 | 0.0231 | 0.0255 | 0.0323 | 0.013 | 0.0173 | 0.0021 | 0.0047 | 0.0221 | 0.0079 | 0.008 |
Golgi | 0.0061 | 0.0023 | 0.0037 | 0.0039 | 0.0068 | 0.0013 | 0.014 | 0.0029 | 0.0085 | 0.0121 | 0.0214 | 0.0024 | 0.0047 | 0.0015 | 0.0035 | 0.0029 | 0.001 | 0.0024 |
Lipid Particles | 0.0055 | 0.0005 | 0.0019 | 0.001 | 0.0013 | 0.0005 | 0.0136 | 0.0006 | 0.0029 | 0.0205 | 0.0423 | 0.0011 | 0.0036 | 0.0006 | 0.0017 | 0.0012 | 0.0005 | 0.0019 |
Mitochondria | 0.0015 | 0.0006 | 0.0009 | 0.0033 | 0.0013 | 0.0007 | 0.0067 | 0.0004 | 0.0144 | 0.0036 | 0.0711 | 0.0011 | 0.0013 | 0.0003 | 0.0008 | 0.0008 | 0.0002 | 0.0005 |
None | 0.3011 | 0.3441 | 0.3541 | 0.1904 | 0.286 | 0.315 | 0.1479 | 0.1433 | 0.1366 | 0.0428 | 0.0746 | 0.0566 | 0.2743 | 0.1911 | 0.2115 | 0.2131 | 0.3824 | 0.1667 |
Nuclear Periphery | 0.0011 | 0.0004 | 0.0005 | 0.0012 | 0.0003 | 0.0004 | 0.0062 | 0.0009 | 0.0037 | 0.0025 | 0.0049 | 0.0041 | 0.0122 | 0.0006 | 0.0007 | 0.0019 | 0.0008 | 0.0007 |
Nucleolus | 0.0004 | 0.0002 | 0.0001 | 0.0003 | 0.0001 | 0.0001 | 0.0023 | 0.0001 | 0.0004 | 0.0002 | 0.0011 | 0.0004 | 0.0005 | 0.0001 | 0.0001 | 0.0001 | 0 | 0.0001 |
Nucleus | 0.0038 | 0.0013 | 0.0008 | 0.0038 | 0.0004 | 0.0009 | 0.0029 | 0.0012 | 0.0021 | 0.0018 | 0.0062 | 0.0023 | 0.0058 | 0.0009 | 0.0008 | 0.0017 | 0.0009 | 0.0011 |
Peroxisomes | 0.0295 | 0.0121 | 0.0185 | 0.0081 | 0.036 | 0.0077 | 0.0221 | 0.0026 | 0.0229 | 0.0347 | 0.0212 | 0.0091 | 0.0176 | 0.0031 | 0.0206 | 0.0043 | 0.0006 | 0.0043 |
Punctate Nuclear | 0.003 | 0.0021 | 0.0018 | 0.0034 | 0.0017 | 0.0011 | 0.0086 | 0.0012 | 0.0055 | 0.005 | 0.0058 | 0.0037 | 0.0054 | 0.0013 | 0.0031 | 0.0025 | 0.0009 | 0.0018 |
Vacuole | 0.0008 | 0.0007 | 0.0005 | 0.0019 | 0.0003 | 0.0004 | 0.0025 | 0.0009 | 0.0013 | 0.0023 | 0.0024 | 0.001 | 0.002 | 0.0005 | 0.0005 | 0.0013 | 0.0006 | 0.0005 |
Vacuole Periphery | 0.0002 | 0.0001 | 0.0001 | 0.0004 | 0.0001 | 0.0001 | 0.0012 | 0.0001 | 0.0006 | 0.0007 | 0.0017 | 0.0003 | 0.0013 | 0.0001 | 0.0001 | 0.0003 | 0.0001 | 0.0001 |
R1 | R2 | |||||||||
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G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 8.6703 | 9.7903 | 4.8432 | 10.8642 | 7.6915 | 9.1215 | 13.6111 | 12.2488 | 10.0266 | 12.1304 |
Translational Efficiency | 0.8462 | 0.8507 | 1.6192 | 0.6337 | 0.9318 | 0.8221 | 0.6462 | 0.8652 | 0.8646 | 0.8125 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
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37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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