Standard name
Human Ortholog
Description Protein serine/threonine phosphatase; regulates Hsp90 chaperone by affecting its ATPase and cochaperone binding activities; has similarity to human phosphatase PP5; present in both the nucleus and cytoplasm; expressed during logarithmic growth
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 – – 0 – – – – 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 – – 0.06 – – – – 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 – – 0 – – – – 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 – – 0 – – – – 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 – – 0 – – – – 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.98 0.97 0.96 1.0 0.97 – – 0.44 – – – – 0.97 0.97 0.9 0.93 0.92 0.89 0.95 0.92 0.92 0.94 0.95 0.86
Endoplasmic Reticulum 0 0 0 0 0 – – 0.06 – – – – 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 – – 0 – – – – 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 – – 0 – – – – 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 – – 0.06 – – – – 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 – – 0 – – – – 0 0 0 0.07 0.11 0.07 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 – – 0 – – – – 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 – – 0.06 – – – – 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 – – 0 – – – – 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 – – 0 – – – – 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0.07 – – 0.44 – – – – 0 0.06 0.21 0.08 0.05 0.11 0 0 0 0 0 0.05
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 – – 0 – – – – 0 0 0 0 0 0 1 0 0 0 0 0
Bud 0 2 0 0 0 – – 1 – – – – 0 0 0 0 0 0 0 0 0 0 0 1
Bud Neck 0 0 0 0 0 – – 0 – – – – 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 – – 0 – – – – 0 0 0 0 0 0 – – – – – –
Cell Periphery 1 1 0 0 0 – – 0 – – – – 3 0 2 10 16 7 0 0 0 0 0 0
Cytoplasm 364 240 25 16 29 – – 8 – – – – 314 283 304 488 348 340 347 236 58 192 222 223
Endoplasmic Reticulum 0 1 1 0 1 – – 1 – – – – 4 3 1 6 8 10 2 0 2 1 1 1
Endosome 0 0 0 0 0 – – 0 – – – – 0 0 1 0 0 2 2 1 0 0 1 2
Golgi 2 0 0 0 0 – – 0 – – – – 0 0 1 0 1 0 2 0 0 0 0 1
Mitochondria 7 2 0 0 0 – – 1 – – – – 1 1 2 5 1 5 2 0 0 1 1 1
Nucleus 2 3 0 0 0 – – 0 – – – – 2 1 2 37 40 28 2 10 0 1 1 7
Nuclear Periphery 0 0 0 0 0 – – 0 – – – – 0 0 0 0 0 0 0 0 0 0 0 1
Nucleolus 0 0 0 0 0 – – 1 – – – – 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 – – 0 – – – – 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 – – 0 – – – – 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 4 0 0 2 – – 8 – – – – 6 18 72 43 20 43 1 4 1 2 1 13
Unique Cell Count 371 247 26 16 30 18 323 292 336 524 379 383 367 257 63 204 234 260
Labelled Cell Count 376 253 26 16 32 20 330 306 385 589 434 435 367 257 63 204 234 260


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 12.8 15.3 10.8 9.4 9.3 – – 6.8 – – – – 13.8 13.8 13.1 15.5 18.9 18.2 12.3 12.0 12.4
Std Deviation (1e-4) 2.1 3.1 1.5 2.2 1.5 – – 1.3 – – – – 2.1 2.4 2.2 4.1 4.8 5.7 2.4 2.4 2.2
Intensity Change (Log2) – – – -0.21 -0.22 – – -0.67 – – – – 0.34 0.35 0.27 0.51 0.8 0.74 0.18 0.15 0.19


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 – – 0 – – – – 0 0 0 0 0 0
Bud 0 0 – – 0 – – – – 0 0 0 0 0 0
Bud Neck 0 0 – – 0 – – – – 0 0 0 0 0 0
Bud Site 0 0 – – 0 – – – – 0 0 0 0 0 0
Cell Periphery 0 0 – – 0 – – – – 0 0 0 0 0 0
Cytoplasm 0.8 0.1 – – -3.9 – – – – 0.3 0.2 -1.0 -0.6 -0.8 -1.2
Endoplasmic Reticulum 0 0 – – 0 – – – – 0 0 0 0 0 0
Endosome 0 0 – – 0 – – – – 0 0 0 0 0 0
Golgi 0 0 – – 0 – – – – 0 0 0 0 0 0
Mitochondria 0 0 – – 0 – – – – 0 0 0 0 0 0
Nucleus 0 0 – – 0 – – – – 0 0 0 0 0 0
Nuclear Periphery 0 0 – – 0 – – – – 0 0 0 0 0 0
Nucleolus 0 0 – – 0 – – – – 0 0 0 0 0 0
Peroxisomes 0 0 – – 0 – – – – 0 0 0 0 0 0
SpindlePole 0 0 – – 0 – – – – 0 0 0 0 0 0
Vacuole 0 0 – – 0 – – – – 0 0 2.6 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 11.6145 15.1359 14.0402 13.3091 13.6309 13.8325 10.7396 13.2693 11.655 10.969 11.1469 11.9199 11.8385 15.4155 13.6487 13.5882 12.0695 13.6272
Actin 0.0421 0.0007 0.0164 0.0003 0.0002 0.0009 0.0051 0.0002 0.008 0.0041 0.0002 0.001 0.0082 0.0035 0.0174 0.0118 0.0203 0.0042
Bud 0.0007 0.0029 0.0013 0.0019 0.0001 0.0001 0.0002 0.0001 0.0002 0.0014 0.0001 0.0002 0.0006 0.0009 0.0004 0.0003 0.0016 0.0001
Bud Neck 0.0012 0.0002 0.001 0.0008 0.0005 0.0012 0.0005 0.0001 0.0013 0.0007 0.0005 0.0011 0.001 0.0003 0.0015 0.0007 0.0016 0.001
Bud Periphery 0.0012 0.0005 0.0005 0.0011 0 0 0.0001 0 0.0002 0.0013 0 0.0003 0.0005 0.0003 0.0004 0.0003 0.0018 0.0001
Bud Site 0.0021 0.001 0.0006 0.0011 0 0.0001 0.0003 0.0001 0.0016 0.0024 0 0.0003 0.0008 0.0009 0.0032 0.0003 0.0027 0.0002
Cell Periphery 0.0003 0.0002 0.0001 0.0001 0 0 0 0 0.0001 0.0001 0 0.0001 0.0001 0 0.0002 0 0.0003 0
Cytoplasm 0.768 0.968 0.9173 0.9456 0.9065 0.9157 0.8922 0.9828 0.9345 0.8728 0.9397 0.9375 0.7673 0.9687 0.8726 0.8493 0.7013 0.8757
Cytoplasmic Foci 0.0295 0.0019 0.0035 0.0028 0.001 0.0133 0.0069 0.0011 0.0034 0.008 0.0017 0.006 0.051 0.0022 0.0127 0.013 0.049 0.0137
Eisosomes 0.0003 0 0.0002 0 0 0 0 0 0.0002 0 0 0 0 0 0.0026 0 0.0001 0
Endoplasmic Reticulum 0.022 0.0023 0.0027 0.0015 0.0013 0.0049 0.0077 0.0007 0.0029 0.0032 0.0013 0.003 0.0094 0.0006 0.0064 0.0007 0.0071 0.0041
Endosome 0.025 0.0005 0.0027 0.0136 0.0005 0.0084 0.0081 0.0004 0.0022 0.0076 0.0008 0.0075 0.0322 0.0003 0.0151 0.0178 0.1029 0.0141
Golgi 0.0057 0.0001 0.0015 0.0013 0 0.0003 0.0029 0 0.0012 0.0014 0 0.0012 0.0041 0.0001 0.0051 0.0157 0.044 0.0056
Lipid Particles 0.008 0.0001 0.0008 0 0 0.0001 0.0013 0 0.0008 0.0007 0 0.0002 0.01 0 0.0022 0.0068 0.0169 0.0072
Mitochondria 0.0027 0.0016 0.0006 0.0056 0.0001 0.0004 0.0009 0.0001 0.0018 0.0027 0.0001 0.0035 0.0027 0.0003 0.0068 0.0107 0.0126 0.0018
None 0.0047 0.004 0.0015 0.0006 0.0012 0.0014 0.0042 0.0006 0.0038 0.0019 0.0009 0.0022 0.011 0.0013 0.0057 0.0014 0.0018 0.0024
Nuclear Periphery 0.0238 0.0021 0.004 0.002 0.0049 0.0054 0.0089 0.001 0.0024 0.0091 0.0027 0.0029 0.0111 0.002 0.0064 0.0036 0.0041 0.0067
Nucleolus 0.0005 0.0003 0.0001 0.0001 0 0 0.0001 0 0.0001 0.0001 0 0.0001 0.0002 0.0001 0.0001 0.0001 0.0002 0.0002
Nucleus 0.0467 0.0117 0.0397 0.0112 0.0821 0.0423 0.0552 0.0123 0.0321 0.0768 0.0505 0.0298 0.0625 0.0158 0.0354 0.0513 0.0168 0.0425
Peroxisomes 0.0032 0.0001 0.004 0.0001 0 0.0002 0.0012 0 0.0015 0.0007 0 0.0002 0.0158 0.0002 0.0029 0.0128 0.0067 0.0103
Punctate Nuclear 0.0054 0.0005 0.0007 0.0002 0.0006 0.0034 0.0028 0.0002 0.0012 0.0015 0.0009 0.0017 0.005 0.0022 0.0012 0.0012 0.0018 0.0083
Vacuole 0.0044 0.0011 0.0006 0.0072 0.0007 0.0013 0.0009 0.0002 0.0004 0.0028 0.0004 0.0009 0.005 0.0002 0.0012 0.0013 0.0048 0.0009
Vacuole Periphery 0.0023 0.0002 0.0002 0.003 0.0002 0.0004 0.0003 0 0.0001 0.0006 0.0001 0.0003 0.0015 0.0001 0.0005 0.0009 0.0015 0.0005

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 55.4564 53.0929 42.2452 52.9407 52.4187 30.7524 45.7592 42.5615 33.8378 49.6082
Translational Efficiency 1.861 1.9089 2.0661 1.3798 1.522 2.5285 1.5704 1.6317 1.6042 1.5738

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
217 1534 461 142 1866 2001 144 185 2083 3535 605 327

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 1161.83 1182.18 1493.47 1732.00 1251.95 1321.57 1845.30 1595.21 1242.56 1261.08 1577.21 1654.61
Standard Deviation 144.93 235.52 220.10 304.43 177.22 224.21 211.39 243.42 176.30 239.37 264.57 279.94
Intensity Change Log 2 — 0.025051 0.362269 0.576040 — 0.078076 0.559678 0.349569 — 0.052796 0.468023 0.463019

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000415 0.000234 0.000553 0.000769 0.000177 0.000154 0.000898 0.000937 0.000202 0.000189 0.000635 0.000864
Bud Neck 0.000567 0.000473 0.003581 0.003001 0.000473 0.000573 0.003244 0.008543 0.000483 0.000530 0.003501 0.006137
Bud Site 0.003952 0.002967 0.005495 0.009577 0.001900 0.003739 0.004855 0.027936 0.002114 0.003404 0.005343 0.019963
Cell Periphery 0.000237 0.000057 0.000335 0.000525 0.000062 0.000036 0.000325 0.000226 0.000081 0.000045 0.000332 0.000356
Cytoplasm 0.785935* 0.867699* 0.498606* 0.757027* 0.830670* 0.855809* 0.556852* 0.523643* 0.826010* 0.860969* 0.512469* 0.624990*
Cytoplasmic Foci 0.055524 0.012724 0.023085 0.041849 0.029744 0.015516 0.014360 0.052560 0.032430 0.014304 0.021008 0.047909
Eisosomes 0.000022 0.000003 0.000067 0.000011 0.000005 0.000003 0.000012 0.000029 0.000007 0.000003 0.000054 0.000021
Endoplasmic Reticulum 0.005392 0.000484 0.004324 0.002332 0.001580 0.000595 0.001594 0.001326 0.001977 0.000547 0.003674 0.001763
Endosome 0.003805 0.000855 0.007478 0.009512 0.000931 0.001423 0.002470 0.037114 0.001231 0.001177 0.006286 0.025128
Golgi 0.000461 0.000786 0.000382 0.010081 0.000520 0.001501 0.000541 0.013192 0.000514 0.001191 0.000420 0.011841
Lipid Particles 0.002294 0.000440 0.003835 0.005117 0.000529 0.000385 0.002698 0.020316 0.000713 0.000409 0.003564 0.013716
Mitochondria 0.000154 0.000122 0.000235 0.021804 0.000419 0.000295 0.000359 0.003465 0.000391 0.000220 0.000264 0.011429
Mitotic Spindle 0.000448 0.002060 0.006509 0.004914 0.002214 0.003031 0.005062 0.045611 0.002030 0.002610 0.006165 0.027938
None 0.001753 0.009345 0.006792 0.002191 0.004451 0.006435 0.003971 0.001126 0.004170 0.007698 0.006121 0.001589
Nuclear Periphery 0.000356 0.000322 0.001366 0.000433 0.000232 0.000149 0.001233 0.000442 0.000245 0.000224 0.001334 0.000438
Nuclear Periphery Foci 0.012229 0.003572 0.010559 0.010354 0.004581 0.002933 0.008195 0.004033 0.005378 0.003211 0.009997 0.006778
Nucleolus 0.000380 0.000254 0.000859 0.000108 0.000124 0.000480 0.000160 0.000583 0.000151 0.000382 0.000693 0.000377
Nucleus 0.091654 0.083799 0.204566* 0.039453 0.103916 0.079659 0.287944* 0.024429 0.102638 0.081456 0.224411* 0.030953
Peroxisomes 0.000243 0.000373 0.000383 0.000477 0.000088 0.000222 0.000095 0.005827 0.000104 0.000287 0.000315 0.003504
Vacuole 0.033790 0.013215 0.220594* 0.069011 0.017110 0.026643 0.104921 0.214903* 0.018848 0.020816 0.193062 0.151549
Vacuole Periphery 0.000388 0.000217 0.000395 0.011453 0.000273 0.000417 0.000213 0.013758 0.000285 0.000330 0.000352 0.012757

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 2.39 -3.98 -1.92 -3.06 0.02 0.73 -8.07 -2.54 -2.80 3.98 0.36 -10.65 -3.93 -4.13 2.17
Bud Neck 0.83 -11.76 -4.97 -5.10 1.84 -2.17 -9.84 -3.66 -3.49 0.78 -1.64 -14.74 -5.60 -5.45 1.74
Bud Site 1.71 -4.86 -6.47 -7.26 -3.70 -2.33 -8.24 -5.20 -4.38 -3.11 -2.65 -10.38 -7.80 -6.80 -4.15
Cell Periphery 6.19 -1.87 -3.65 -6.54 -2.80 6.25 -9.77 -4.37 -5.68 6.97 6.99 -12.77 -6.73 -7.82 1.05
Cytoplasm -5.69 18.84 3.33 7.43 -10.23 -2.30 19.51 13.89 14.41 -3.21 -5.85 35.09 13.60 15.27 -8.04
Cytoplasmic Foci 7.90 6.87 1.43 -5.49 -4.55 8.69 5.39 -1.76 -5.03 -4.66 11.83 8.45 -2.24 -7.43 -6.37
Eisosomes 6.89 -4.33 3.84 -4.48 7.24 3.36 -8.40 -3.45 -4.10 1.89 6.99 -8.98 -3.23 -5.52 6.74
Endoplasmic Reticulum 3.04 1.24 2.66 -0.83 4.27 4.87 -1.31 4.83 0.28 5.16 5.92 -3.18 3.96 -0.66 7.25
Endosome 4.00 -2.35 0.06 -2.15 1.48 -2.18 -5.75 -2.93 -2.80 -2.32 0.58 -10.22 -3.24 -3.29 -1.28
Golgi -1.00 0.65 -1.37 -1.24 -1.39 -1.84 -0.93 -1.53 -1.09 -1.45 -1.91 0.25 -2.04 -1.64 -2.07
Lipid Particles 2.66 -1.80 -3.01 -4.88 -2.31 1.88 -8.27 -1.90 -1.94 -0.82 3.00 -11.97 -2.95 -3.08 -1.52
Mitochondria 0.46 -2.65 -1.24 -1.25 -1.20 0.55 -0.06 -1.08 -1.55 -1.20 0.82 0.07 -1.32 -1.40 -1.33
Mitotic Spindle -2.10 -4.79 -1.93 -1.58 0.14 -0.80 -2.30 -2.81 -2.67 -1.67 -0.70 -4.41 -3.23 -3.10 -1.34
None -9.35 -2.71 0.18 9.73 2.82 -2.27 2.61 5.63 10.48 4.25 -5.06 -0.04 5.43 14.12 3.54
Nuclear Periphery 0.23 -11.87 -0.08 -0.28 12.47 1.40 -10.32 -2.79 -5.16 9.23 0.23 -17.15 -2.48 -1.65 15.81
Nuclear Periphery Foci 5.75 1.54 1.64 -2.93 0.49 3.01 -3.77 1.69 -0.74 4.41 4.52 -4.96 -0.47 -2.93 3.46
Nucleolus 0.60 -1.00 1.31 2.39 6.08 -2.06 -3.99 -1.36 1.18 -0.53 -2.21 -5.33 -0.63 1.70 3.94
Nucleus 0.60 -10.28 4.22 5.80 16.20 6.12 -9.88 13.39 10.09 13.71 6.01 -15.57 13.93 11.10 22.49
Peroxisomes -0.57 -0.91 -0.29 0.40 0.25 -2.10 -3.30 -2.18 -1.98 -2.11 -1.98 -6.34 -2.32 -1.85 -1.95
Vacuole 3.80 -18.14 -6.79 -8.37 6.53 -8.62 -11.34 -14.33 -13.35 -2.85 -5.36 -23.66 -15.41 -14.77 0.76
Vacuole Periphery 1.61 0.38 -0.97 -1.02 -0.98 -1.26 0.39 -2.15 -2.07 -2.17 -0.39 -0.72 -1.98 -1.97 -1.96
Standard name
Human Ortholog
Description Protein serine/threonine phosphatase; regulates Hsp90 chaperone by affecting its ATPase and cochaperone binding activities; has similarity to human phosphatase PP5; present in both the nucleus and cytoplasm; expressed during logarithmic growth
Localization
Cell Percentages cytoplasm (100%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-7

Ppt1

Ppt1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ppt1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available