Standard name
Human Ortholog
Description Putative protein of unknown function; deletion mutant has decreased rapamycin resistance but normal wormannin resistance; green fluorescent protein (GFP)-fusion protein localizes to the vacuole
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0.11 0.06 – 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
Cytoplasm 0.22 0.43 0.13 0.3 0.36 0.44 0.49 0.47 0.59 0.59 0.51 0.59 0.32 0.23 0.42 0.59 0.6 – 0 0 0 0 0 0
Endoplasmic Reticulum 0.24 0.31 0.42 0.6 0.55 0.5 0.44 0.36 0.21 0.27 0.29 0.26 0.36 0.33 0.32 0.07 0 – 0.38 0.44 0.67 0.23 0.27 0.3
Endosome 0.06 0 0.12 0 0 0 0 0 0 0 0 0 0 0 0 0.11 0.09 – 0.05 0.05 0.05 0.05 0.05 0.07
Golgi 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0.07 0.16 0.2 0.24 0.17 0 0 0 0.09 0.07 – 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
Vac/Vac Membrane 0.59 0.29 0.42 0.07 0.14 0.08 0.09 0.14 0.08 0.08 0 0.06 0.41 0.47 0.34 0.16 0.16 – 0.5 0.42 0.21 0.66 0.61 0.54
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 4 3 3 9 11 12 8 27 10 8 8 5 0 0 1 5 4 – 0 0 0 0 1 0
Bud 0 1 1 3 1 3 4 7 6 9 4 6 0 0 0 0 2 – 2 1 1 0 0 2
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 1 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – – –
Cell Periphery 0 0 0 0 0 2 4 2 5 4 9 13 0 0 0 1 3 – 0 0 0 0 0 0
Cytoplasm 79 56 26 67 108 146 216 244 182 221 149 238 7 10 16 26 41 – 5 6 2 1 0 2
Endoplasmic Reticulum 84 40 84 132 166 164 193 188 66 101 84 105 8 14 12 3 2 – 156 72 169 28 34 49
Endosome 22 2 24 7 8 9 16 16 13 9 4 5 0 1 0 5 6 – 19 9 12 6 6 10
Golgi 0 0 5 11 11 0 3 5 1 4 0 1 0 0 0 1 0 – 6 1 4 1 1 2
Mitochondria 0 0 3 3 2 14 18 38 50 75 70 69 0 0 0 4 5 – 2 0 2 0 0 1
Nucleus 1 0 0 1 1 0 0 2 0 1 2 0 0 0 1 0 0 – 0 0 0 0 0 0
Nuclear Periphery 0 1 0 1 0 1 2 7 0 0 1 3 0 0 0 0 0 – 9 1 4 1 4 2
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 – 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
Vac/Vac Membrane 209 38 85 16 41 28 39 74 26 30 12 24 9 20 13 7 11 – 202 70 54 81 79 88
Unique Cell Count 353 130 202 221 300 331 442 521 311 372 290 405 22 43 38 44 68 407 166 254 124 131 163
Labelled Cell Count 399 141 231 250 349 379 503 610 359 462 343 469 24 45 43 52 75 407 166 254 124 131 163


Vacuole

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 10.6 9.8 10.8 12.4 14.1 10.1 9.9 9.2 8.6 8.0 7.9 7.8 11.0 10.4 10.4 8.7 9.3 – 11.4 11.1 11.2
Std Deviation (1e-4) 1.7 1.7 2.1 2.5 2.7 2.3 1.7 1.6 1.8 1.7 1.6 1.4 1.4 1.5 1.4 1.2 1.7 – 2.1 2.3 1.6
Intensity Change (Log2) – – – 0.2 0.39 -0.09 -0.12 -0.22 -0.32 -0.43 -0.45 -0.47 0.03 -0.05 -0.05 -0.31 -0.21 – 0.09 0.05 0.06


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 1.6 1.5 1.4 0 2.2 1.2 0 0 0 0 0 0 0 0 –
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 –
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 –
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 –
Cytoplasm 4.3 5.7 7.5 8.8 8.5 10.3 10.8 8.8 10.7 0 1.7 4.4 6.8 7.8 –
Endoplasmic Reticulum 3.7 3.0 1.8 0.5 -1.4 -5.0 -3.5 -2.9 -3.9 -0.5 -1.1 -1.2 -4.4 -5.9 –
Endosome -3.4 -4.1 -4.3 -4.0 -4.6 -3.3 -4.7 -4.9 -5.8 0 0 0 -0.1 -0.7 –
Golgi 1.3 0.7 0 0 0 0 0 0 0 0 0 0 0 0 –
Mitochondria 0 0 1.7 1.7 3.0 5.3 6.2 7.0 5.6 0 0 0 0 0 –
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 –
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 –
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 –
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 –
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 –
Vacuole -8.4 -7.2 -9.2 -9.9 -8.1 -9.1 -9.7 -10.4 -10.9 -0.1 0.5 -0.9 -3.3 -3.9 –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration – – – – – – – – – – – – – – – – – –
Actin 0.0319 0.0005 0.0019 0.0114 0.0106 0.0008 0.0723 0.0005 0.0068 0.0027 0.0004 0.0003 – – – – – –
Bud 0.0009 0.0004 0.0002 0.0006 0.0007 0.0005 0.0006 0.0006 0.0003 0.0017 0.0003 0.0002 – – – – – –
Bud Neck 0.001 0.0007 0.0005 0.0052 0.0005 0.0009 0.0009 0.0005 0.0005 0.002 0.0004 0.0009 – – – – – –
Bud Periphery 0.0015 0.001 0.0003 0.0006 0.0016 0.0008 0.0011 0.0018 0.0007 0.0023 0.0005 0.0005 – – – – – –
Bud Site 0.0042 0.0024 0.0021 0.0285 0.0005 0.0005 0.0028 0.0011 0.0006 0.0018 0.0007 0.0005 – – – – – –
Cell Periphery 0.0015 0.0051 0.0016 0.0009 0.0014 0.0008 0.0024 0.0032 0.0027 0.0031 0.0011 0.002 – – – – – –
Cytoplasm 0.1169 0.1926 0.0965 0.1192 0.0265 0.0728 0.0306 0.1737 0.0894 0.1382 0.0681 0.0639 – – – – – –
Cytoplasmic Foci 0.0126 0.0185 0.0317 0.0293 0.0106 0.0089 0.0145 0.0104 0.0193 0.0217 0.0151 0.0127 – – – – – –
Eisosomes 0.0006 0 0.0001 0.0002 0.0001 0 0.0008 0 0.0001 0.0001 0 0 – – – – – –
Endoplasmic Reticulum 0.2603 0.3578 0.3309 0.1794 0.0881 0.3673 0.4746 0.384 0.4545 0.2462 0.176 0.44 – – – – – –
Endosome 0.1639 0.0771 0.2265 0.3317 0.1814 0.1708 0.1251 0.1142 0.112 0.2222 0.3269 0.1705 – – – – – –
Golgi 0.0358 0.0026 0.0181 0.0132 0.0062 0.0049 0.0158 0.0048 0.0055 0.0208 0.0187 0.0113 – – – – – –
Lipid Particles 0.0219 0.0456 0.0218 0.0163 0.0064 0.0145 0.0312 0.0245 0.038 0.0233 0.0086 0.012 – – – – – –
Mitochondria 0.014 0.0017 0.0035 0.0028 0.0074 0.003 0.0149 0.0034 0.0182 0.0271 0.0064 0.0036 – – – – – –
None 0.0342 0.0056 0.0103 0.0026 0.0011 0.0125 0.0039 0.0047 0.0052 0.0044 0.0018 0.0008 – – – – – –
Nuclear Periphery 0.099 0.0627 0.0457 0.0633 0.1153 0.0852 0.0477 0.0416 0.0623 0.0344 0.0359 0.0525 – – – – – –
Nucleolus 0.0009 0.0003 0.0007 0.0004 0.0004 0.0003 0.0003 0.0003 0.0006 0.0058 0.0002 0.0003 – – – – – –
Nucleus 0.0136 0.025 0.0083 0.0054 0.0085 0.0092 0.0024 0.0047 0.0047 0.0074 0.0022 0.0036 – – – – – –
Peroxisomes 0.0011 0.0002 0.0089 0.0041 0.0002 0.0027 0.0079 0.0003 0.008 0.0036 0.0004 0.0003 – – – – – –
Punctate Nuclear 0.0023 0.0013 0.0033 0.001 0.0012 0.0006 0.0023 0.0008 0.0013 0.0086 0.0003 0.0003 – – – – – –
Vacuole 0.1603 0.1872 0.169 0.1505 0.4542 0.2053 0.1355 0.2089 0.1544 0.1941 0.2856 0.2012 – – – – – –
Vacuole Periphery 0.0217 0.0116 0.0181 0.0334 0.0774 0.0377 0.0122 0.0161 0.0149 0.0284 0.0505 0.0226 – – – – – –

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 17.9113 12.0483 15.7633 25.2369 21.8432 13.8248 22.0399 21.7452 21.6286 24.702
Translational Efficiency 0.739 1.1115 0.9147 0.6078 0.5805 1.0113 0.5887 0.613 0.642 0.5427

Hit Data

Dataset Hit
Protein Concentration –
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
2318 981 237 943 1634 421 226 1517 3952 1402 463 2460

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 926.10 1009.07 1029.85 1115.01 1199.22 986.89 1145.24 1038.77 1039.02 1002.41 1086.17 1068.00
Standard Deviation 111.06 167.23 124.92 164.80 219.24 178.56 126.24 155.30 212.60 171.01 138.18 163.27
Intensity Change Log 2 — 0.123786 0.153194 0.267817 — -0.281135 -0.066446 -0.207220 — -0.090597 0.033395 0.019191

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000241 0.000371 0.000580 0.001447 0.003198 0.000238 0.000161 0.000487 0.001463 0.000331 0.000376 0.000855
Bud Neck 0.003907 0.000769 0.005758 0.007581 0.000258 0.001176 0.002358 0.004006 0.002398 0.000891 0.004099 0.005376
Bud Site 0.004564 0.004998 0.008534 0.028241 0.005511 0.005526 0.002677 0.013733 0.004955 0.005157 0.005675 0.019294
Cell Periphery 0.000173 0.000834 0.000204 0.000839 0.006990 0.000555 0.000247 0.000430 0.002991 0.000750 0.000225 0.000587
Cytoplasm 0.052098 0.040852 0.069575 0.077267 0.009641 0.033419 0.038126 0.070692 0.034544 0.038620 0.054224 0.073213
Cytoplasmic Foci 0.034299 0.046295 0.041605 0.042465 0.005220 0.047502 0.011172 0.057456 0.022276 0.046657 0.026750 0.051709
Eisosomes 0.000047 0.000048 0.000025 0.000065 0.000491 0.000044 0.000016 0.000035 0.000231 0.000047 0.000021 0.000046
Endoplasmic Reticulum 0.216004* 0.650395* 0.078048 0.118197* 0.938651* 0.453662* 0.425380* 0.195252* 0.514791* 0.591319* 0.247588* 0.165714*
Endosome 0.075558 0.052793 0.070368 0.082343 0.004712 0.063815 0.051968 0.106080 0.046266 0.056103 0.061386 0.096981
Golgi 0.004109 0.012723 0.005175 0.010164 0.003319 0.008651 0.002839 0.010317 0.003782 0.011500 0.004035 0.010258
Lipid Particles 0.028751 0.014605 0.016376 0.028162 0.007127 0.018588 0.011421 0.028140 0.019810 0.015801 0.013957 0.028148
Mitochondria 0.004655 0.002366 0.000858 0.001163 0.001854 0.006092 0.000230 0.001870 0.003497 0.003485 0.000551 0.001599
Mitotic Spindle 0.003320 0.000303 0.017372 0.006778 0.000077 0.001910 0.001621 0.003688 0.001979 0.000785 0.009684 0.004872
None 0.000174 0.001994 0.000317 0.000922 0.002732 0.000803 0.000061 0.000299 0.001232 0.001636 0.000192 0.000538
Nuclear Periphery 0.021310 0.001577 0.024701 0.003794 0.000174 0.005186 0.019952 0.004572 0.012571 0.002661 0.022383 0.004274
Nuclear Periphery Foci 0.015725 0.012239 0.157167 0.029875 0.000932 0.014633 0.105078 0.029681 0.009608 0.012958 0.131742 0.029755
Nucleolus 0.001434 0.000667 0.003812 0.000477 0.000261 0.000763 0.000243 0.000360 0.000949 0.000696 0.002070 0.000405
Nucleus 0.017339 0.003425 0.019242 0.006206 0.002030 0.003271 0.005752 0.004598 0.011010 0.003379 0.012657 0.005214
Peroxisomes 0.000998 0.001004 0.000765 0.000936 0.004236 0.001023 0.000118 0.000600 0.002337 0.001010 0.000449 0.000729
Vacuole 0.496161* 0.116661 0.376868* 0.536154* 0.001957 0.262881* 0.307995* 0.439847* 0.291827* 0.160569 0.343250* 0.476764*
Vacuole Periphery 0.019134 0.035081 0.102648* 0.016925 0.000629 0.070260 0.012584 0.027858 0.011483 0.045645 0.058686 0.023667

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -1.48 -3.60 -4.14 -3.67 -2.80 4.64 4.76 4.17 -2.57 -3.45 4.21 3.99 2.09 -4.04 -3.59
Bud Neck 12.20 -3.12 -6.96 -12.91 -2.71 -5.02 -7.27 -16.74 -10.48 -4.58 9.08 -4.82 -11.04 -17.50 -3.35
Bud Site -0.50 -3.14 -7.88 -7.70 -5.92 0.00 2.50 -5.54 -4.57 -8.53 -0.22 -1.14 -9.99 -9.60 -8.81
Cell Periphery -9.11 -1.59 -3.57 0.44 -3.33 7.72 8.09 7.87 1.71 -2.33 6.36 7.92 6.86 2.24 -4.17
Cytoplasm 3.53 -2.06 -6.33 -8.23 -1.55 -5.82 -4.65 -17.17 -7.77 -4.74 -1.06 -3.71 -13.68 -10.91 -3.80
Cytoplasmic Foci -3.49 -1.81 -2.53 1.40 0.10 -9.06 -3.38 -21.82 -1.82 -16.14 -8.95 -1.94 -15.37 -1.35 -8.96
Eisosomes -0.10 5.52 -2.46 -1.75 -6.10 5.77 6.14 5.90 1.62 -6.16 5.66 6.50 5.75 0.28 -8.08
Endoplasmic Reticulum -36.66* 13.47 12.72 44.16* -2.64 26.81* 22.99 94.56* 14.14 9.89 -6.74 17.24 42.71 41.10* 5.86
Endosome 6.90 1.06 0.01 -5.59 -0.95 -13.29 -10.70 -33.97* -7.36 -9.68 -4.02 -4.43 -18.27 -11.76 -8.15
Golgi -6.87 -1.11 -3.21 2.26 -2.39 -4.03 0.33 -6.67 -0.81 -3.90 -8.10 -0.20 -6.83 1.77 -4.80
Lipid Particles 7.83 4.84 -0.17 -6.42 -4.38 -4.10 -2.44 -11.96 -3.09 -7.18 2.74 3.50 -5.95 -7.48 -7.83
Mitochondria 2.84 5.29 4.92 3.09 -0.78 -1.94 3.71 0.12 1.99 -5.97 -0.02 6.45 4.08 2.76 -4.93
Mitotic Spindle 3.72 -3.46 -2.89 -5.04 2.07 -1.04 -1.55 -4.74 -1.58 -2.36 1.73 -3.54 -4.34 -5.42 1.47
None -2.07 -1.58 -0.96 1.10 -0.74 1.66 2.65 2.41 0.93 -2.41 -0.53 2.49 1.54 1.69 -1.14
Nuclear Periphery 14.10* -1.11 11.53 -4.29 7.54 -4.76 -6.36 -10.16 0.44 4.85 11.02 -4.42 9.02 -3.67 8.64
Nuclear Periphery Foci 2.20 -11.16 -6.32 -7.45 9.73 -4.32 -9.69 -14.59 -4.34 6.82 -2.19 -14.49 -12.92 -9.11 11.78
Nucleolus 1.92 -2.49 4.66 0.39 3.54 -1.26 0.07 -0.46 1.18 -1.13 0.87 -2.21 3.98 0.98 3.42
Nucleus 9.17 -0.60 8.12 -3.08 3.70 -1.22 -2.48 -3.32 -2.60 0.91 7.26 -1.05 6.50 -2.96 3.86
Peroxisomes 0.00 1.55 0.89 0.32 -0.64 4.78 6.46 5.69 1.98 -7.94 3.31 6.79 5.93 1.05 -2.72
Vacuole 45.23* 7.15 -4.21 -39.10 -9.21 -20.19 -15.83 -59.21* -12.20 -6.38 16.69 -3.85 -23.90 -38.92 -9.98
Vacuole Periphery -6.54 -6.80 2.78 7.76 7.14 -12.04 -5.12 -20.70 7.41 -5.44 -13.87 -7.03 -10.05 9.19 5.33
Standard name
Human Ortholog
Description Putative protein of unknown function; deletion mutant has decreased rapamycin resistance but normal wormannin resistance; green fluorescent protein (GFP)-fusion protein localizes to the vacuole
Localization
Cell Percentages ER (68%)
Cell Cycle Regulation No
Subcompartmental Group ER-3

Vsb1

Vsb1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Vsb1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available