Standard name
Human Ortholog
Description Member of the NineTeen Complex (NTC); NTC contains Prp19p and stabilizes U6 snRNA in catalytic forms of the spliceosome containing U2, U5, and U6 snRNAs; relocalizes to the cytosol in response to hypoxia; isy1 syf2 cells have defective spindles activiating cell cycle arrest
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0.06 0.05 0.06 0 – 0 0 0 0 0.1 0.11 0.15 0.07 0.1 0.07 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.25 0 – 0.14 0.09 0.13 0.11 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0.96 0.96 0.95 0.94 0.93 0.85 0.92 – 0.88 0.85 0.87 0.88 0.89 0.88 0.8 0.85 0.84 0.9 0.89 0.95 0.93 0.84 0.75 0.66
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0.05 0.05 – 0.06 0.1 0.11 0.09 0 0 0 0 0 0 0 0 0 0 0.06 0.07
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0.08 0.13 0.19
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0
Bud 0 0 0 0 0 3 0 – 0 2 4 4 1 0 1 1 0 0 2 1 0 0 3 3
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 2 7 2 – 8 12 20 28 0 0 1 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 6 3 8 14 19 19 11 – 10 6 13 8 21 48 56 8 10 6 0 0 0 3 1 3
Endoplasmic Reticulum 0 0 0 0 0 0 0 – 0 0 0 0 1 0 1 5 1 1 0 0 0 0 1 1
Endosome 1 0 1 0 0 0 3 – 0 1 0 0 0 1 1 1 1 0 3 0 2 0 5 3
Golgi 1 0 0 3 0 0 0 – 0 0 0 0 0 0 3 0 0 0 6 0 0 1 1 0
Mitochondria 3 7 0 1 1 76 14 – 56 40 75 71 0 0 2 1 2 1 1 2 1 2 6 5
Nucleus 278 167 207 238 354 259 417 – 339 370 493 567 190 404 298 93 84 79 269 172 210 206 303 231
Nuclear Periphery 0 0 0 1 4 0 18 – 6 10 4 12 0 1 3 0 0 0 1 0 0 0 0 2
Nucleolus 4 1 2 1 1 15 21 – 23 44 60 60 1 5 8 1 1 1 2 0 2 6 23 24
Peroxisomes 0 0 0 0 0 0 1 – 0 0 0 0 0 3 4 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 2 – 2 0 0 1 2 1 3 1 0 1 2 0 0 0 3 2
Vac/Vac Membrane 4 2 2 1 8 3 7 – 3 12 6 4 0 0 3 4 6 1 7 2 4 18 51 65
Unique Cell Count 291 174 217 254 379 305 454 387 434 564 643 214 457 373 109 100 88 303 182 226 245 406 351
Labelled Cell Count 297 180 220 259 389 382 496 447 497 675 755 216 463 384 115 105 90 303 182 226 245 406 351


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 8.2 7.6 7.9 7.6 7.6 4.9 6.7 – 6.0 6.1 5.9 6.0 8.5 7.8 8.9 10.8 11.6 11.6 7.8 8.1 8.5
Std Deviation (1e-4) 1.5 1.4 1.5 1.9 1.6 1.4 1.3 – 1.4 1.3 1.4 1.2 2.0 1.9 2.6 3.0 2.8 2.8 1.5 1.7 1.9
Intensity Change (Log2) – – – -0.05 -0.05 -0.68 -0.25 – -0.4 -0.37 -0.41 -0.4 0.1 -0.02 0.17 0.44 0.55 0.55 -0.02 0.04 0.11


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 – 0 0 2.8 3.1 0 0 0 0 0 0
Cell Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.9 0.7 1.3 -0.9 – -0.8 0 -1.1 0 2.5 3.0 4.3 1.4 2.3 0
Endoplasmic Reticulum 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 8.0 0 – 5.9 4.6 5.6 5.1 0 0 0 0 0 0
Nucleus -0.8 -1.0 -3.8 -1.7 – -3.1 -3.8 -3.3 -3.1 -2.5 -2.9 -5.2 -3.2 -3.4 -1.8
Nuclear Periphery 0 0 0 3.0 – 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 2.5 2.5 – 3.0 4.3 4.5 4.1 0 0 0 0 0 0
Peroxisomes 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 3.102 2.8553 2.4523 2.6562 1.9753 2.7853 5.1887 5.0935 5.0093 4.3158 4.7141 4.91 7.1153 7.0001 7.0491 6.9149 6.6302 7.0371
Actin 0.0036 0 0.005 0.0131 0.0037 0.0004 0.0324 0 0.0007 0 0.0058 0.0065 0.0007 0 0 0 0.001 0
Bud 0.0001 0 0.0003 0.0003 0.0002 0 0.0005 0 0.0004 0 0.0003 0 0.0001 0 0 0 0.0002 0
Bud Neck 0.0006 0 0.0002 0.0001 0.0004 0.0005 0.0052 0 0.0004 0.0001 0.0005 0.0004 0.0004 0 0 0 0.0003 0.0003
Bud Periphery 0.0001 0 0.0005 0.001 0.0004 0.0001 0.0012 0 0.0013 0 0.0005 0.0001 0.0005 0 0 0 0.001 0
Bud Site 0.0004 0 0.0017 0.001 0.0006 0.0001 0.0068 0 0.0005 0 0.0007 0 0.0004 0 0 0 0.0002 0
Cell Periphery 0.0001 0 0.0002 0.0002 0.0002 0 0.0006 0 0.0002 0 0.0002 0 0.0002 0 0 0 0.0002 0
Cytoplasm 0.0128 0.0001 0.0004 0.0001 0.002 0.0003 0.0023 0.0052 0.0004 0.0001 0.0002 0 0.0001 0 0 0.0001 0.0001 0
Cytoplasmic Foci 0.0057 0.0052 0.0055 0.0013 0.0197 0.0008 0.0116 0.0003 0.0013 0 0.0187 0.0002 0.0001 0 0 0 0 0
Eisosomes 0.0006 0 0.0001 0.0004 0 0 0.001 0 0 0 0.0001 0 0.0001 0 0 0 0.0001 0
Endoplasmic Reticulum 0.0033 0 0.0005 0.0008 0.0015 0.0002 0.0036 0.0002 0.0006 0 0.0004 0 0.0002 0 0 0 0.0005 0
Endosome 0.01 0.001 0.0079 0.0009 0.0123 0.0018 0.0157 0.0004 0.0065 0 0.031 0.0003 0.0005 0 0 0 0.0007 0
Golgi 0.0023 0.0003 0.0028 0.001 0.004 0.0005 0.0085 0 0.0039 0 0.0432 0.0011 0.0002 0 0 0 0 0
Lipid Particles 0.0111 0.0024 0.0161 0.0079 0.0225 0.0101 0.0139 0 0.0063 0 0.0219 0.001 0.0016 0 0 0 0.0001 0
Mitochondria 0.002 0.0002 0.0052 0.0109 0.0249 0.0014 0.0243 0.0001 0.0036 0.0001 0.0862 0.0002 0.0048 0.0001 0.0001 0.0001 0.0948 0.0001
None 0.0123 0 0.0022 0.0006 0.0011 0.0031 0.0119 0.0025 0.0002 0.0001 0.0001 0 0.0002 0 0 0 0.0002 0
Nuclear Periphery 0.0141 0.0052 0.0041 0.0042 0.0156 0.003 0.0073 0.005 0.0069 0.0048 0.0004 0.0002 0.0025 0.0003 0.0004 0.0007 0.0247 0.0001
Nucleolus 0.0093 0.0121 0.0062 0.0067 0.0254 0.0245 0.0048 0.0044 0.0056 0.0102 0.0208 0.0145 0.0141 0.0169 0.0029 0.0017 0.0249 0.0098
Nucleus 0.8932 0.9702 0.9301 0.9451 0.7922 0.9476 0.8188 0.9806 0.9559 0.9739 0.7292 0.9739 0.971 0.9824 0.9964 0.9974 0.8337 0.9894
Peroxisomes 0.0024 0.0016 0.006 0.002 0.0156 0.0004 0.014 0 0.0004 0 0.031 0.0003 0.0002 0 0 0 0.0001 0
Punctate Nuclear 0.0136 0.001 0.0011 0.0011 0.0385 0.0045 0.0094 0.0004 0.0021 0.0105 0.0004 0.001 0.0006 0.0002 0 0.0001 0.0002 0.0001
Vacuole 0.0013 0.0001 0.0021 0.0005 0.0126 0.0003 0.0039 0.0007 0.0019 0 0.004 0.0001 0.0008 0 0 0 0.0025 0
Vacuole Periphery 0.001 0.0001 0.0017 0.0009 0.0064 0.0004 0.0022 0.0001 0.001 0.0001 0.0043 0 0.0007 0 0 0 0.0144 0

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 2.5542 10.6553 7.9067 7.0371 7.7271 1.7018 6.5411 9.8684 7.049 7.9665
Translational Efficiency – – – – – – – – – –

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency –

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
723 1502 374 1958 2190 2467 1582 2159 2913 3969 1956 4117

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 791.90 976.54 1142.97 1117.20 837.24 990.46 1149.63 1291.87 825.99 985.19 1148.36 1208.80
Standard Deviation 127.17 200.19 147.16 198.00 115.18 144.85 159.28 201.60 119.88 168.09 157.06 218.10
Intensity Change Log 2 — 0.302361 0.529397 0.496497 — 0.242457 0.457456 0.625748 — 0.271886 0.492874 0.564366

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000499 0.000533 0.000905 0.000721 0.000150 0.000352 0.000468 0.000540 0.000237 0.000420 0.000551 0.000626
Bud Neck 0.004779 0.009237 0.010671 0.019031 0.003362 0.012304 0.016591 0.026575 0.003713 0.011144 0.015459 0.022987
Bud Site 0.001004 0.001462 0.001061 0.002506 0.000314 0.001796 0.000899 0.001739 0.000485 0.001670 0.000930 0.002104
Cell Periphery 0.000392 0.000573 0.000053 0.000177 0.000136 0.000359 0.000071 0.000105 0.000200 0.000440 0.000067 0.000139
Cytoplasm 0.004230 0.004466 0.000273 0.002758 0.001358 0.002172 0.000257 0.001501 0.002071 0.003040 0.000260 0.002099
Cytoplasmic Foci 0.002581 0.001035 0.000025 0.000431 0.000290 0.000816 0.000004 0.000506 0.000858 0.000899 0.000008 0.000470
Eisosomes 0.000038 0.000045 0.000044 0.000035 0.000021 0.000046 0.000045 0.000033 0.000026 0.000046 0.000045 0.000034
Endoplasmic Reticulum 0.008534 0.008281 0.004173 0.002710 0.005859 0.008562 0.003045 0.002741 0.006523 0.008456 0.003261 0.002727
Endosome 0.000736 0.000845 0.000098 0.000477 0.000289 0.001056 0.000056 0.001033 0.000400 0.000976 0.000064 0.000769
Golgi 0.000379 0.000559 0.000062 0.000588 0.000078 0.000999 0.000020 0.000685 0.000153 0.000833 0.000028 0.000639
Lipid Particles 0.002416 0.000772 0.000069 0.000400 0.000117 0.000091 0.000009 0.000047 0.000687 0.000349 0.000021 0.000215
Mitochondria 0.001281 0.001005 0.001097 0.001885 0.000409 0.001681 0.001062 0.002890 0.000625 0.001425 0.001069 0.002412
Mitotic Spindle 0.000321 0.000655 0.000576 0.006240 0.000637 0.002055 0.000071 0.004765 0.000558 0.001525 0.000168 0.005466
None 0.008187 0.014809 0.001158 0.006274 0.002622 0.003597 0.000754 0.001904 0.004003 0.007840 0.000831 0.003982
Nuclear Periphery 0.000774 0.000638 0.000200 0.000652 0.000373 0.000697 0.000329 0.001372 0.000473 0.000675 0.000304 0.001029
Nuclear Periphery Foci 0.001594 0.000571 0.000057 0.000391 0.000335 0.000263 0.000015 0.000390 0.000648 0.000380 0.000023 0.000391
Nucleolus 0.016857 0.014052 0.009432 0.004140 0.006484 0.009146 0.004022 0.005241 0.009058 0.011003 0.005056 0.004718
Nucleus 0.942262* 0.937493* 0.968527* 0.947791* 0.976313* 0.950181* 0.971717* 0.943608* 0.967862* 0.945379* 0.971107* 0.945598*
Peroxisomes 0.000655 0.000283 0.000423 0.000115 0.000099 0.000207 0.000076 0.000118 0.000237 0.000236 0.000143 0.000117
Vacuole 0.001723 0.001710 0.000987 0.001877 0.000485 0.002601 0.000415 0.002821 0.000792 0.002264 0.000524 0.002372
Vacuole Periphery 0.000759 0.000976 0.000110 0.000800 0.000266 0.001018 0.000075 0.001388 0.000389 0.001002 0.000081 0.001108

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -0.30 -6.91 -4.80 -3.29 3.14 -8.23 -14.15 -18.12 -8.48 -3.73 -5.67 -14.03 -17.34 -7.69 -3.19
Bud Neck -4.69 -5.05 -12.91 -9.83 -5.77 -10.18 -15.40 -19.55 -11.15 -7.24 -11.80 -15.98 -24.50 -14.14 -7.59
Bud Site -1.41 -1.00 -4.31 -2.35 -3.52 -5.20 -3.78 -7.66 -0.41 -3.17 -5.50 -3.63 -9.00 -1.96 -4.71
Cell Periphery -0.82 2.93 2.09 2.20 -4.16 -4.52 3.50 2.22 5.48 -1.93 -2.88 4.13 2.37 4.04 -4.17
Cytoplasm -0.16 4.09 1.00 1.16 -5.25 -1.56 2.93 -0.85 1.01 -4.78 -1.77 4.90 -0.76 1.27 -7.06
Cytoplasmic Foci 1.83 3.25 2.71 1.40 -2.15 -1.65 1.62 -0.31 1.25 -1.69 0.01 3.60 1.72 1.87 -2.70
Eisosomes -0.95 -1.97 1.01 1.94 4.04 -8.24 -10.17 -5.94 4.94 6.14 -6.92 -9.31 -4.05 4.54 6.40
Endoplasmic Reticulum 0.34 5.43 8.35 11.86 5.26 -6.03 9.70 10.70 15.46 2.28 -5.04 11.27 13.59 19.51 4.42
Endosome -0.28 4.03 2.05 1.74 -2.45 -3.17 3.27 -2.09 0.72 -3.13 -3.14 5.12 -1.41 1.66 -4.04
Golgi -0.32 1.73 -0.01 0.28 -1.36 -1.99 1.09 -2.12 0.90 -2.44 -2.14 2.09 -1.78 0.95 -2.70
Lipid Particles 1.56 2.52 2.24 0.67 -0.99 0.46 2.12 1.34 2.47 -4.83 1.12 2.82 2.04 0.65 -1.40
Mitochondria 1.15 0.55 -2.45 -4.74 -4.25 -3.70 -6.40 -5.14 -2.06 -3.61 -3.46 -4.68 -6.72 -3.07 -4.86
Mitotic Spindle -1.16 -0.86 -4.98 -4.43 -4.57 -2.26 1.58 -3.70 -2.06 -4.34 -2.36 1.33 -5.91 -4.20 -6.61
None -2.18 3.49 1.19 3.82 -4.97 -1.13 2.79 0.90 2.40 -3.91 -3.31 4.46 0.33 4.00 -6.28
Nuclear Periphery 0.27 1.19 0.00 -0.66 -4.60 -3.50 0.22 -3.64 -2.32 -3.51 -1.53 0.99 -3.25 -2.34 -4.35
Nuclear Periphery Foci 1.81 2.02 2.06 0.78 -0.22 0.32 1.84 0.05 -0.42 -3.52 1.39 2.86 1.49 0.14 -3.21
Nucleolus 1.59 2.95 6.76 7.72 2.38 -3.33 3.24 -0.50 2.36 -3.04 -2.55 4.96 3.91 6.57 -1.45
Nucleus 0.60 -3.69 -0.34 -1.23 4.66 9.02 3.45 11.45 2.80 9.54 8.31 -0.36 9.98 1.41 11.39
Peroxisomes 2.32 1.38 3.41 2.52 5.08 -2.47 0.85 -1.48 1.75 -3.04 0.22 2.05 2.65 3.01 1.49
Vacuole 0.05 -0.41 -0.94 -1.19 -0.21 -5.15 0.05 -4.36 -0.14 -4.40 -4.73 -0.05 -4.63 -0.65 -4.35
Vacuole Periphery -0.35 1.48 0.26 0.73 -2.39 -3.09 2.09 -3.27 -0.40 -4.20 -2.50 2.39 -2.46 0.34 -4.87
Standard name
Human Ortholog
Description Member of the NineTeen Complex (NTC); NTC contains Prp19p and stabilizes U6 snRNA in catalytic forms of the spliceosome containing U2, U5, and U6 snRNAs; relocalizes to the cytosol in response to hypoxia; isy1 syf2 cells have defective spindles activiating cell cycle arrest
Localization
Cell Percentages nucleus (99%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-4

Syf2

Syf2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Syf2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available