Standard name
Human Ortholog
Description Subunit of the prohibitin complex (Phb1p-Phb2p); prohibitin is a 1.2 MDa ring-shaped inner mitochondrial membrane chaperone that stabilizes newly synthesized proteins; determinant of replicative life span; involved in mitochondrial segregation; prohibitin deficiency induces a mitochondrial unfolded protein response (mtUPR)

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0.06 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0.08 0.11 0.05 0 0 0 0 0 0
Cytoplasm 0 0.13 0.11 0.06 0.05 0.08 0.06 0 0.05 0 0 0 0.21 0.39 0.54 0.14 0.12 0.15 0 0 0 0.06 0.09 0.1
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0.21 0.1 0 0.15 0.07 0.11 0 0 0 0 0.22 0.18 0.22 0.14 0.09 0.06 0.08 0.08 0.07 0.09 0.09 0.09
Golgi 0 0 0.07 0.07 0 0 0 0 0 0 0 0 0.12 0 0 0.26 0.41 0.38 0.05 0 0 0 0 0
Mitochondria 0.8 0.75 0.47 0.78 0.82 0.7 0.75 0.72 0.9 0.91 0.88 0.91 0.23 0.21 0.12 0.08 0.07 0.1 0.64 0.64 0.7 0.55 0.45 0.46
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0.05 0 0 0.07 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.06 0 0 0 0 0.06 0 0 0 0.06 0.06 0 0 0 0 0 0 0.07 0 0 0 0 0 0
Peroxisomes 0 0 0.06 0 0 0 0 0 0 0 0 0 0 0.09 0.05 0.09 0.05 0 0 0 0.06 0 0 0
SpindlePole 0 0 0.09 0 0 0 0.05 0.06 0 0.06 0 0 0 0.05 0 0 0 0.05 0 0 0 0 0 0
Vac/Vac Membrane 0.24 0.22 0.17 0.12 0.06 0.18 0.12 0.19 0 0.05 0.05 0 0.24 0.15 0.12 0.26 0.23 0.24 0.06 0.12 0 0.1 0.11 0.12
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 2 2 2 3 0 0 1 0 0 0 0 3 4 0 3 5 4 3 4 2 3 5 6
Bud 1 12 4 4 1 2 2 9 11 6 10 5 1 0 1 10 3 2 2 2 0 3 6 3
Bud Neck 0 0 0 0 0 0 0 1 1 0 0 0 0 0 0 0 0 0 0 5 1 3 5 7
Bud Site 0 1 0 1 1 0 0 0 3 9 2 2 0 0 0 0 0 0
Cell Periphery 3 5 2 4 2 1 2 2 2 0 1 1 5 7 2 20 19 12 0 2 0 0 0 0
Cytoplasm 3 40 16 10 7 17 13 5 10 6 8 4 22 62 108 34 21 34 0 11 1 10 27 29
Endoplasmic Reticulum 4 5 1 2 4 2 1 2 3 2 1 2 4 7 3 10 6 5 0 0 1 1 4 4
Endosome 2 11 32 15 5 30 16 26 2 1 2 0 23 29 45 35 16 14 13 28 14 16 27 26
Golgi 2 1 10 11 4 3 1 2 0 0 1 1 12 5 0 63 74 87 8 5 5 4 11 11
Mitochondria 108 238 70 122 119 140 168 170 175 193 159 129 24 33 24 19 13 23 102 228 141 101 138 131
Nucleus 5 9 5 0 5 5 6 5 4 6 5 5 1 0 1 1 2 7 0 7 2 2 4 2
Nuclear Periphery 3 1 1 0 4 4 11 5 8 14 4 5 0 0 0 3 0 1 2 3 1 1 5 6
Nucleolus 8 1 1 0 5 13 9 9 5 13 10 5 0 0 1 5 6 15 2 0 1 2 6 1
Peroxisomes 0 4 9 4 1 9 1 1 0 4 0 0 4 14 10 23 9 8 5 3 11 2 3 4
SpindlePole 1 8 14 5 4 7 12 14 3 12 4 4 3 8 4 7 1 12 3 2 2 3 13 8
Vac/Vac Membrane 32 69 26 19 9 37 27 46 5 11 9 4 25 24 24 63 41 56 9 41 7 17 35 33
Unique Cell Count 135 316 150 157 145 201 225 237 194 211 181 141 104 159 201 244 180 230 159 355 203 183 308 284
Labelled Cell Count 172 407 193 199 174 270 269 298 232 277 216 167 127 193 223 296 216 280 159 355 203 183 308 284


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 6.1 4.6 6.3 6.6 5.2 6.8 6.3 6.1 5.5 5.0 5.2 5.0 6.9 6.2 5.5 8.0 10.9 10.3 7.3 7.4 7.4
Std Deviation (1e-4) 1.4 0.8 1.5 1.3 1.4 1.8 1.5 1.2 2.0 1.2 1.3 1.2 1.6 1.2 1.4 2.2 3.3 2.6 2.3 2.3 2.0
Intensity Change (Log2) 0.06 -0.27 0.09 -0.01 -0.05 -0.22 -0.34 -0.29 -0.34 0.12 -0.02 -0.21 0.33 0.79 0.69 0.2 0.22 0.22


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin
Bud 0 0 0
Bud Neck 0 0 0
Bud Site 0 0 0
Cell Periphery 0 0 0
Cyto
Endoplasmic Reticulum 0 0 0
Endosome 0 0 0
Golgi 0 0 0
Mitochondria 0 0 0
Nuclear Periphery 0 0 0
Nuc
Nucleolus 0 0 0
Peroxisomes 0 0 0
SpindlePole 0 0 0
Vac
Cortical Patches 0 0 0
Cytoplasm 0 0 0
Nucleus 0 0 0
Vacuole 2.0 1.2 1.6

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 5.3451 6.3504 5.7069 5.3095 4.9854 6.0935 9.0715 9.4193 8.6084 9.2502 9.4172 9.644 7.3049 6.8528 7.3156 6.3687 6.5053 7.0252
Actin 0.0298 0.0392 0.0267 0.0056 0.0563 0.0256 0.051 0.0408 0.0456 0.0251 0.1203 0.0822 0.0115 0.0054 0.0297 0.0303 0.0167 0.0124
Bud 0.0007 0.0028 0.0021 0.002 0.0009 0.0005 0.0046 0.0297 0.0025 0.0004 0.0307 0.0014 0.0013 0.0067 0.0034 0.0074 0.0049 0.0017
Bud Neck 0.0017 0.0007 0.0025 0.002 0.0005 0.0194 0.0165 0.007 0.0025 0.0002 0.0002 0.0075 0.0014 0.0025 0.0017 0.0017 0.0069 0.004
Bud Periphery 0.0014 0.0032 0.0034 0.0099 0.0011 0.0009 0.0152 0.0271 0.0039 0.0009 0.0871 0.0024 0.0024 0.0083 0.0045 0.0291 0.009 0.003
Bud Site 0.0078 0.0087 0.0129 0.0035 0.0016 0.0012 0.0178 0.1966 0.0159 0.0005 0.0045 0.0019 0.003 0.0472 0.0102 0.0088 0.0099 0.0015
Cell Periphery 0.0014 0.0005 0.0012 0.0037 0.0002 0.0004 0.0038 0.0023 0.0007 0.0002 0.0003 0.0005 0.0006 0.002 0.0009 0.0025 0.0006 0.0006
Cytoplasm 0.002 0.0068 0.0016 0.0004 0.0014 0.0006 0.0144 0.0023 0.0138 0.0001 0.0006 0.0017 0.0073 0.0023 0.0016 0.0011 0.0018 0.0011
Cytoplasmic Foci 0.0451 0.0403 0.0315 0.0075 0.0447 0.019 0.0488 0.052 0.0396 0.0048 0.0007 0.0323 0.0512 0.0324 0.0224 0.0216 0.0433 0.0252
Eisosomes 0.0007 0.0004 0.0005 0.0002 0.0001 0.0002 0.0006 0.0004 0.0003 0.0004 0.0002 0.0005 0.0003 0.0002 0.0003 0.0002 0.0002 0.0002
Endoplasmic Reticulum 0.0022 0.0016 0.0007 0.0004 0.0002 0.0005 0.0058 0.0005 0.0012 0.0001 0.0005 0.0007 0.0021 0.0006 0.0036 0.0006 0.001 0.0007
Endosome 0.0726 0.0902 0.0649 0.0347 0.0631 0.048 0.125 0.0826 0.0463 0.0116 0.0019 0.0732 0.1075 0.1064 0.0558 0.0809 0.0799 0.065
Golgi 0.0823 0.1189 0.0747 0.0499 0.0823 0.1309 0.0706 0.1112 0.0758 0.0278 0.0031 0.1855 0.1106 0.0911 0.0831 0.0993 0.0864 0.1403
Lipid Particles 0.0538 0.0045 0.0162 0.0055 0.0081 0.0212 0.0303 0.0374 0.0223 0.0168 0.0005 0.0395 0.034 0.0183 0.0144 0.0124 0.0183 0.0247
Mitochondria 0.5967 0.6071 0.6984 0.8304 0.6792 0.6895 0.3747 0.3315 0.6384 0.8879 0.7455 0.4948 0.5847 0.5774 0.6888 0.631 0.6387 0.6592
None 0.0027 0.0003 0.0011 0.0002 0.0001 0.0001 0.0439 0.0004 0.0003 0.0001 0.0002 0.0003 0.0038 0.0002 0.0003 0.0004 0.002 0.0004
Nuclear Periphery 0.0021 0.0004 0.0005 0.0001 0.0001 0.0003 0.0306 0.0002 0.0037 0.0001 0.0006 0.0008 0.0012 0.0003 0.0005 0.0006 0.0043 0.0042
Nucleolus 0.0093 0.0004 0.0011 0.0018 0.0002 0.0006 0.0059 0.0032 0.0009 0.0002 0.0001 0.0004 0.0007 0.0142 0.0102 0.001 0.0031 0.0033
Nucleus 0.002 0.0006 0.0006 0.0008 0.0002 0.0002 0.0148 0.0012 0.0068 0.0002 0.0002 0.0003 0.0007 0.0019 0.0013 0.0006 0.0018 0.0015
Peroxisomes 0.0588 0.0475 0.0353 0.0175 0.0508 0.0325 0.0351 0.0473 0.0682 0.0171 0.0009 0.065 0.0438 0.029 0.0396 0.0402 0.0439 0.0309
Punctate Nuclear 0.0073 0.0002 0.0008 0.0001 0.0002 0.0001 0.0128 0.0004 0.0006 0 0.0002 0.0004 0.0011 0.0003 0.0003 0.0003 0.0058 0.0008
Vacuole 0.01 0.0184 0.0139 0.0169 0.004 0.0035 0.0433 0.0195 0.0068 0.0026 0.0007 0.0041 0.0166 0.0428 0.0208 0.0196 0.017 0.0119
Vacuole Periphery 0.0096 0.0075 0.0095 0.0071 0.0045 0.0047 0.0346 0.0064 0.0039 0.0031 0.0011 0.0045 0.0142 0.0107 0.0066 0.0102 0.0046 0.0075

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 97.0587 94.756 94.8803 103.2042 92.7249 91.8995 111.9681 128.938 139.6926 112.5043
Translational Efficiency 1.5724 1.5249 1.5354 1.6135 1.4134 1.8309 1.6174 1.6427 1.4951 1.3562

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
122 337 124 1583 2025 2447 2768 1692 2147 2784 2892 3275

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 667.81 912.03 1161.65 1081.83 784.76 938.84 1121.44 1099.25 778.11 935.59 1123.16 1090.83
Standard Deviation 84.97 142.48 184.16 176.12 99.84 128.33 183.50 172.61 102.69 130.42 183.71 174.54
Intensity Change Log 2 0.449644 0.798666 0.695964 0.258628 0.515029 0.486196 0.349596 0.652380 0.586435

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000494 0.003400 0.020714 0.005065 0.000414 0.000672 0.001603 0.003884 0.000419 0.001002 0.002423 0.004455
Bud Neck 0.027831 0.008727 0.017173 0.009428 0.004710 0.004527 0.005790 0.004494 0.006024 0.005035 0.006278 0.006879
Bud Site 0.018349 0.050795 0.039128 0.123380* 0.011589 0.014327 0.050532 0.077797 0.011973 0.018741 0.050043 0.099830
Cell Periphery 0.000953 0.000709 0.000946 0.000559 0.000299 0.000324 0.000530 0.000482 0.000336 0.000371 0.000548 0.000519
Cytoplasm 0.005599 0.008152 0.003122 0.032467 0.004047 0.003170 0.009844 0.023003 0.004135 0.003773 0.009556 0.027577
Cytoplasmic Foci 0.038088 0.073847 0.096754 0.060270 0.034269 0.025478 0.022000 0.055248 0.034486 0.031333 0.025205 0.057675
Eisosomes 0.000103 0.000110 0.000148 0.000062 0.000059 0.000058 0.000056 0.000058 0.000061 0.000064 0.000060 0.000060
Endoplasmic Reticulum 0.000439 0.000846 0.002787 0.001204 0.001080 0.000361 0.000667 0.001157 0.001043 0.000420 0.000758 0.001180
Endosome 0.048365 0.111327 0.152867 0.078966 0.084414 0.078650 0.066634 0.081854 0.082366 0.082605 0.070332 0.080458
Golgi 0.045548 0.135837* 0.106806 0.109403 0.097786 0.084534 0.078965 0.134244* 0.094818 0.090744 0.080159 0.122237*
Lipid Particles 0.002321 0.002393 0.003854 0.000560 0.003562 0.001421 0.000366 0.000543 0.003491 0.001538 0.000516 0.000551
Mitochondria 0.714978* 0.459980* 0.238725* 0.211790* 0.655452* 0.706245* 0.510358* 0.309843* 0.658835* 0.676435* 0.498711* 0.262448*
Mitotic Spindle 0.005652 0.037115 0.138571* 0.190741* 0.009433 0.010562 0.065853 0.112056* 0.009218 0.013776 0.068971 0.150089*
None 0.000560 0.000301 0.000304 0.000538 0.000366 0.000751 0.000752 0.000539 0.000377 0.000697 0.000733 0.000538
Nuclear Periphery 0.000053 0.000157 0.001741 0.000367 0.000383 0.000105 0.000705 0.000390 0.000364 0.000112 0.000749 0.000379
Nuclear Periphery Foci 0.000085 0.000217 0.003427 0.000807 0.000400 0.000393 0.000748 0.001409 0.000382 0.000371 0.000862 0.001118
Nucleolus 0.001388 0.000270 0.003316 0.000418 0.000856 0.000398 0.000866 0.000308 0.000886 0.000382 0.000971 0.000361
Nucleus 0.002282 0.000696 0.004563 0.001024 0.001294 0.000893 0.000971 0.000819 0.001350 0.000869 0.001125 0.000918
Peroxisomes 0.049507 0.055037 0.072406 0.013590 0.012296 0.014414 0.005700 0.008898 0.014410 0.019331 0.008560 0.011166
Vacuole 0.017982 0.028679 0.053816 0.105370 0.036103 0.029230 0.098334 0.105443 0.035074 0.029163 0.096425 0.105408
Vacuole Periphery 0.019422 0.021404 0.038833 0.053992 0.041188 0.023488 0.078726 0.077534 0.039951 0.023236 0.077016 0.066155

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -2.58 -2.61 -5.10 -1.17 2.01 -1.61 -5.60 -4.33 -3.98 -2.78 -2.94 -5.10 -6.78 -5.61 -2.87
Bud Neck 2.10 0.98 2.01 -0.63 2.54 0.25 -1.73 0.01 -0.30 2.36 1.13 -0.49 -1.38 -3.76 -1.37
Bud Site -3.99 -2.82 -17.25 -8.74 -10.42 -1.68 -15.94 -18.23 -17.40 -6.67 -3.88 -16.15 -28.58 -25.53 -13.89
Cell Periphery 0.91 0.02 1.61 0.93 0.80 -0.88 -6.35 -3.17 -2.75 0.83 -1.01 -4.97 -2.71 -2.17 0.53
Cytoplasm -0.90 0.94 -9.49 -10.89 -15.89 1.33 -7.00 -12.48 -13.33 -8.29 0.52 -6.79 -19.10 -20.00 -13.95
Cytoplasmic Foci -3.69 -4.66 -2.60 2.34 3.69 4.93 7.47 -8.18 -12.16 -14.17 1.69 5.52 -10.87 -12.78 -17.03
Eisosomes -0.29 -2.08 3.03 2.80 5.12 0.15 0.84 0.34 0.14 -0.54 -0.75 0.39 0.56 1.28 0.15
Endoplasmic Reticulum -1.88 -4.31 -4.23 -1.78 2.94 2.49 1.34 -0.31 -5.79 -3.19 2.28 0.94 -0.56 -7.72 -3.51
Endosome -5.74 -7.22 -3.75 3.94 5.90 1.46 4.97 0.78 -0.75 -4.57 -0.05 3.49 0.82 0.96 -3.47
Golgi -7.29 -4.03 -6.77 2.88 -0.02 3.00 4.40 -6.17 -9.06 -10.30 0.97 3.58 -5.55 -7.01 -9.84
Lipid Particles -0.06 -1.58 2.70 1.95 4.21 2.46 4.12 3.79 2.76 -2.18 2.38 4.05 3.92 3.32 -1.23
Mitochondria 7.37 12.34 17.48 12.00 1.13 -5.41 15.15 33.48* 40.03* 19.54* -1.89 17.09 46.71* 51.25* 27.99*
Mitotic Spindle -5.53 -7.02 -26.51* -17.77 -2.58 -0.99 -19.27 -20.36 -20.13 -8.10 -3.66 -20.41 -32.74 -31.37* -16.01
None 1.24 1.22 0.15 -4.75 -4.32 -1.87 -8.22 -3.60 1.08 5.41 -1.76 -7.72 -3.60 0.95 5.76
Nuclear Periphery -1.12 -2.71 -5.45 -2.11 2.17 2.95 -1.96 -0.11 -6.18 2.19 2.84 -2.43 -0.30 -6.40 2.60
Nuclear Periphery Foci -2.76 -2.98 -5.01 -4.07 2.26 -0.18 -1.95 -1.75 -1.66 -1.13 -0.15 -2.73 -2.44 -2.25 -0.86
Nucleolus 1.34 -1.18 1.12 -1.76 1.90 1.48 -0.11 1.84 0.92 2.99 1.71 -0.35 1.78 0.04 3.15
Nucleus 1.57 -1.21 1.02 -1.68 1.81 0.89 0.68 0.79 -0.19 0.21 1.16 0.38 0.60 -0.95 0.30
Peroxisomes -0.40 -1.28 3.10 5.30 4.39 -1.30 5.12 2.36 3.93 -3.23 -2.58 3.85 2.21 5.31 -2.57
Vacuole -1.72 -4.69 -14.80 -13.34 -7.67 3.05 -20.02 -18.24 -20.79 -2.05 2.68 -20.52 -23.79 -27.18 -3.25
Vacuole Periphery -0.39 -2.56 -6.59 -8.08 -2.13 8.69 -12.61 -10.44 -17.39 0.50 8.61 -12.95 -9.64 -19.23 3.95
Standard name
Human Ortholog
Description Subunit of the prohibitin complex (Phb1p-Phb2p); prohibitin is a 1.2 MDa ring-shaped inner mitochondrial membrane chaperone that stabilizes newly synthesized proteins; determinant of replicative life span; involved in mitochondrial segregation; prohibitin deficiency induces a mitochondrial unfolded protein response (mtUPR)
Localization
Cell Percentages mitochondrion (96%)
Cell Cycle Regulation No
Subcompartmental Group mito-2

Phb1

Phb1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Phb1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available