Standard name
Human Ortholog
Description Subunit of the CCR4-NOT transcriptional regulatory complex; CCR4-NOT complex is evolutionarily-conserved and involved in controlling mRNA initiation, elongation, and degradation
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches – – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – 0 0 0 0 0
Bud – – 0 0 0 0 0 0 0 – 0.06 0 0 0 0 0 0 0 – 0 0 0 0 0
Bud Neck – – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – 0 0 0 0 0
Bud Site – – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery – – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0.07 0 – 0 0 0 0 0
Cytoplasm – – 0.99 0.96 0.93 0.85 0.82 0.8 0.7 – 0.57 0.61 0.98 1.0 0.99 0.92 0.92 0.96 – 0 0.73 0.83 0.74 0.73
Endoplasmic Reticulum – – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0.05 0.05 – 0 0 0.05 0.05 0.07
Endosome – – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – 0 0 0 0 0
Golgi – – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – 0 0 0 0 0
Mitochondria – – 0 0.18 0.14 0.39 0.39 0.37 0.62 – 0.67 0.7 0 0 0 0 0 0 – 0 0.15 0.08 0.12 0.07
Nucleus – – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – 0 0 0 0 0
Nuclear Periphery – – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – 0.09 0 0 0 0
Nucleolus – – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – 0 0 0 0 0
Peroxisomes – – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – 0 0 0 0 0
SpindlePole – – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – 0 0 0 0 0
Vac/Vac Membrane – – 0 0 0 0 0 0 0 – 0 0 0 0 0 0.05 0.05 0 – 0.81 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches – – 0 0 0 0 0 1 1 – 0 0 1 0 0 0 0 1 – 0 1 0 2 1
Bud – – 0 2 1 5 11 10 9 – 15 7 2 1 8 0 0 0 – 0 2 0 3 1
Bud Neck – – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – 0 2 0 0 3
Bud Site – – 0 0 0 0 0 2 1 – 0 3 0 0 0 0 0 0 – – – – – –
Cell Periphery – – 4 2 6 4 2 6 5 – 7 6 3 1 4 9 16 6 – 0 2 0 1 0
Cytoplasm – – 358 276 350 299 353 368 243 – 146 213 236 266 264 324 215 276 – 0 275 168 163 181
Endoplasmic Reticulum – – 0 0 0 0 0 2 1 – 1 0 2 0 0 13 11 15 – 0 7 9 11 16
Endosome – – 0 1 4 2 2 1 0 – 0 0 1 0 0 7 4 3 – 0 6 0 1 4
Golgi – – 0 0 1 0 0 0 0 – 0 0 0 0 0 4 3 1 – 0 0 0 0 1
Mitochondria – – 7 51 52 139 167 170 214 – 172 247 0 1 5 10 1 9 – 0 57 17 25 17
Nucleus – – 0 2 0 4 1 4 1 – 1 1 0 0 1 2 2 2 – 0 1 0 0 1
Nuclear Periphery – – 0 0 0 0 0 0 0 – 1 1 0 0 0 0 0 0 – 0 0 0 0 0
Nucleolus – – 0 0 0 0 0 0 0 – 1 0 0 0 0 1 0 0 – 0 0 0 0 0
Peroxisomes – – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – 0 0 0 0 0
SpindlePole – – 0 0 0 0 3 1 1 – 3 5 0 0 0 1 0 1 – 0 0 0 0 1
Vac/Vac Membrane – – 3 0 5 1 3 3 2 – 2 4 0 0 0 19 12 5 – 0 11 5 4 8
Unique Cell Count 363 289 376 353 430 462 345 256 351 240 267 268 353 234 289 1 379 203 221 248
Labelled Cell Count 372 334 419 454 542 568 478 349 487 245 269 282 390 264 319 1 379 203 221 248


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) – – 4.4 4.4 4.4 4.0 3.8 4.0 3.3 – 3.3 3.1 5.9 5.7 5.2 7.3 7.5 7.1 6.3 6.4 6.6
Std Deviation (1e-4) – – 0.8 1.6 1.2 1.5 1.2 1.6 1.3 – 1.0 1.0 1.0 0.9 0.8 1.6 1.6 1.8 1.0 1.4 1.4
Intensity Change (Log2) – – – 0.02 0.02 -0.12 -0.2 -0.13 -0.39 – -0.4 -0.49 0.44 0.39 0.26 0.75 0.77 0.71 0.53 0.55 0.59


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 – 0 0 0 0 0 1.5 3.8 0
Cytoplasm -2.4 -3.8 -6.8 -7.6 -8.3 -10.5 – -13.1 -12.7 -0.3 1.3 -0.1 -4.3 -4.1 -2.4
Endoplasmic Reticulum 0 0 0 0 0 0 – 0 0 0 0 0 3.7 0 4.4
Endosome 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 – 0 0 0 0 -0.1 0.8 0 1.0
Nucleus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 – 0 0 0 0 0 3.5 3.3 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 3.2886 2.8552 2.6226 2.3835 3.2671 2.4948 -0.4099 0.705 0.5071 0.1205 -0.3651 0.4917 4.3714 5.0825 4.633 4.9709 4.7087 4.8483
Actin 0.1405 0.0006 0.0379 0.0002 0.1196 0.0116 0.001 0.0026 0.0019 0.003 0.0023 0.0006 0.023 0.0073 0.0089 0.0095 0.0584 0.0007
Bud 0.002 0.0059 0.0017 0.0001 0.0015 0.0001 0.0016 0.01 0.0089 0.0048 0.0035 0.0015 0.0005 0.0045 0.0016 0.0003 0.0012 0.0001
Bud Neck 0.0076 0.0002 0.001 0.0001 0.005 0.0007 0.0027 0.0008 0.0032 0.001 0.0005 0.0017 0.011 0.0003 0.0003 0.0497 0.0017 0.0005
Bud Periphery 0.0034 0.0031 0.0019 0.0001 0.002 0.0002 0.0018 0.0052 0.0061 0.0027 0.0056 0.0015 0.0007 0.0031 0.0014 0.0004 0.0013 0.0001
Bud Site 0.0132 0.0147 0.003 0.0001 0.0078 0.0002 0.0128 0.0375 0.0245 0.0107 0.0011 0.0005 0.0096 0.0114 0.0041 0.0014 0.0065 0.0001
Cell Periphery 0.0014 0.0012 0.0007 0.0001 0.0006 0.0002 0.0007 0.001 0.0009 0.0006 0.0002 0.0002 0.0007 0.0005 0.0003 0.0005 0.0002 0.0001
Cytoplasm 0.3141 0.7571 0.6967 0.7131 0.4816 0.7916 0.6116 0.6571 0.5927 0.6194 0.6919 0.6934 0.6058 0.6825 0.6344 0.6454 0.6197 0.7434
Cytoplasmic Foci 0.0505 0.0134 0.0099 0.0096 0.0443 0.0174 0.04 0.037 0.0422 0.0564 0.0332 0.0449 0.0255 0.0292 0.0332 0.0306 0.0413 0.017
Eisosomes 0.0043 0.0002 0.0002 0.0001 0.0009 0.0001 0.0001 0.0001 0.0001 0 0.0001 0 0.0003 0.0001 0.0004 0.0014 0.0002 0
Endoplasmic Reticulum 0.0407 0.0075 0.0067 0.0028 0.0142 0.0053 0.0117 0.0068 0.0094 0.0087 0.0056 0.0081 0.0106 0.0059 0.008 0.0013 0.0137 0.0061
Endosome 0.055 0.0055 0.0117 0.013 0.0545 0.0116 0.0418 0.0352 0.0516 0.0774 0.0336 0.0448 0.0326 0.0183 0.037 0.0095 0.0176 0.012
Golgi 0.0163 0.0004 0.0033 0.0004 0.0224 0.0042 0.0021 0.0025 0.0028 0.0072 0.0011 0.0012 0.0082 0.0022 0.0038 0.0042 0.0059 0.0012
Lipid Particles 0.0235 0.0005 0.0015 0.0002 0.0206 0.0043 0.002 0.0006 0.0011 0.0017 0.001 0.0004 0.0113 0.0011 0.0014 0.0081 0.0038 0.0004
Mitochondria 0.0403 0.0003 0.0057 0.0002 0.0244 0.0033 0.0023 0.0022 0.0034 0.0026 0.0026 0.0017 0.0028 0.0009 0.0119 0.0027 0.0031 0.0005
None 0.1878 0.1835 0.2016 0.2532 0.1581 0.1433 0.2466 0.1812 0.2208 0.15 0.2035 0.1836 0.2139 0.2264 0.2426 0.2112 0.1975 0.2148
Nuclear Periphery 0.0297 0.0007 0.0052 0.0007 0.0081 0.0004 0.0031 0.0025 0.0035 0.0044 0.002 0.0014 0.0206 0.0009 0.0031 0.0003 0.0019 0.0005
Nucleolus 0.0042 0.0001 0.0001 0 0.002 0.0001 0.0007 0.0008 0.0012 0.0015 0.0005 0.0003 0.0007 0.0001 0.0001 0.0013 0.0002 0
Nucleus 0.0191 0.0009 0.0021 0.001 0.0037 0.0006 0.0038 0.005 0.0088 0.0162 0.0025 0.003 0.0065 0.0011 0.0009 0.0007 0.0011 0.0006
Peroxisomes 0.0189 0.0001 0.0028 0 0.0068 0.002 0.0004 0.0005 0.0006 0.0005 0.0008 0.0003 0.0077 0.0005 0.0015 0.0186 0.0196 0.0001
Punctate Nuclear 0.0169 0.0004 0.001 0.0002 0.016 0.0005 0.0021 0.0027 0.0042 0.0091 0.0019 0.003 0.0034 0.0008 0.0016 0.001 0.0012 0.0004
Vacuole 0.0067 0.0033 0.0042 0.004 0.0033 0.0018 0.01 0.0077 0.0104 0.0196 0.0052 0.0068 0.0032 0.0025 0.0027 0.0016 0.0036 0.0012
Vacuole Periphery 0.0039 0.0002 0.001 0.0007 0.0025 0.0005 0.0013 0.0012 0.0018 0.0026 0.0013 0.0013 0.0013 0.0003 0.0007 0.0003 0.0005 0.0002

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 9.8255 13.7607 11.2973 15.1095 9.7667 16.6941 16.5037 15.4845 15.1226 15.2011
Translational Efficiency 0.4965 0.3862 0.4845 0.3715 0.3849 0.2868 0.3245 0.3882 0.303 0.2652

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
86 105 148 1300 1587 1208 145 946 1673 1313 293 2246

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 710.42 822.95 1057.67 854.81 653.56 731.77 1000.41 835.83 656.48 739.06 1029.33 846.82
Standard Deviation 76.95 90.11 105.44 112.17 89.25 85.35 107.67 109.12 89.54 89.24 110.33 111.29
Intensity Change Log 2 — 0.212133 0.574145 0.266932 — 0.163071 0.614200 0.354890 — 0.188832 0.593477 0.309747

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000114 0.000628 0.000707 0.000468 0.000144 0.000509 0.000302 0.001782 0.000142 0.000519 0.000507 0.001021
Bud Neck 0.027152 0.035824 0.002060 0.006405 0.009946 0.025616 0.002932 0.006205 0.010831 0.026433 0.002492 0.006321
Bud Site 0.016507 0.053939 0.007014 0.025844 0.005906 0.030430 0.005506 0.034205 0.006451 0.032310 0.006267 0.029365
Cell Periphery 0.000152 0.000343 0.000309 0.000109 0.000123 0.000119 0.000161 0.000176 0.000125 0.000137 0.000236 0.000137
Cytoplasm 0.368770* 0.269463* 0.453445* 0.615632* 0.408508* 0.384440* 0.454693* 0.581049* 0.406466* 0.375245* 0.454062* 0.601066*
Cytoplasmic Foci 0.396764* 0.388419* 0.062203 0.057572 0.322359* 0.328318* 0.039110 0.071561 0.326184* 0.333124* 0.050775 0.063464
Eisosomes 0.000212 0.000239 0.000098 0.000033 0.000128 0.000149 0.000076 0.000050 0.000133 0.000156 0.000087 0.000040
Endoplasmic Reticulum 0.000776 0.000851 0.007058 0.000984 0.004131 0.001595 0.003992 0.000890 0.003959 0.001536 0.005541 0.000944
Endosome 0.047283 0.082916 0.018596 0.009637 0.042979 0.054699 0.006723 0.009657 0.043200 0.056955 0.012720 0.009645
Golgi 0.009548 0.018870 0.000344 0.004303 0.010174 0.018785 0.000106 0.007205 0.010142 0.018792 0.000226 0.005525
Lipid Particles 0.006131 0.004558 0.007021 0.000990 0.020528 0.008367 0.003381 0.001024 0.019788 0.008063 0.005220 0.001005
Mitochondria 0.003280 0.004679 0.000165 0.002920 0.010650 0.014221 0.000126 0.002146 0.010271 0.013458 0.000146 0.002594
Mitotic Spindle 0.000155 0.004974 0.002689 0.030101 0.000563 0.004248 0.000480 0.011650 0.000542 0.004306 0.001595 0.022330
None 0.000273 0.000279 0.000963 0.002470 0.003187 0.001191 0.004611 0.001700 0.003037 0.001118 0.002769 0.002146
Nuclear Periphery 0.000082 0.000090 0.001620 0.000517 0.001162 0.000297 0.001374 0.000339 0.001106 0.000281 0.001498 0.000442
Nuclear Periphery Foci 0.000294 0.000627 0.019233 0.001863 0.000788 0.000462 0.008046 0.002179 0.000762 0.000475 0.013697 0.001996
Nucleolus 0.000250 0.000307 0.000482 0.000314 0.001403 0.001487 0.000239 0.000229 0.001343 0.001393 0.000362 0.000278
Nucleus 0.015346 0.009856 0.106587 0.063150 0.047105 0.021248 0.154496* 0.053810 0.045472 0.020337 0.130296* 0.059216
Peroxisomes 0.005098 0.018198 0.000625 0.001726 0.003894 0.009834 0.000305 0.001925 0.003955 0.010503 0.000467 0.001810
Vacuole 0.098549 0.099492 0.308269* 0.173041 0.100173 0.087554 0.313158* 0.210047* 0.100089 0.088509 0.310688* 0.188627
Vacuole Periphery 0.003264 0.005447 0.000513 0.001921 0.006150 0.006429 0.000183 0.002170 0.006001 0.006351 0.000350 0.002026

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -5.41 -7.78 -5.04 2.77 4.79 -5.31 -5.56 -2.99 -1.76 -2.32 -5.88 -8.67 -3.78 -0.84 -0.50
Bud Neck -0.74 4.97 4.53 3.56 -2.64 -8.63 3.69 4.50 11.28 -0.28 -8.47 6.99 6.41 12.56 -1.36
Bud Site -2.78 2.06 0.38 3.08 -4.07 -10.45 -0.35 -5.29 2.38 -4.36 -10.81 -0.49 -6.78 5.13 -5.78
Cell Periphery -2.12 -4.61 3.44 3.13 8.87 0.46 -1.26 -0.02 -0.19 0.68 -1.11 -6.29 2.73 3.10 7.25
Cytoplasm 3.02 -0.76 -5.18 -10.95 -5.11 2.27 3.14 -7.57 -8.94 -6.72 3.11 2.41 -11.12 -13.25 -8.42
Cytoplasmic Foci 0.69 14.51 15.14 14.78 1.82 -0.70 46.34 45.38 38.56 -6.01 -0.71 48.13 51.80 43.75 -2.18
Eisosomes -0.44 4.10 6.83 6.00 9.20 -1.86 6.30 13.94 10.18 4.63 -2.13 6.13 17.45 12.36 10.14
Endoplasmic Reticulum -0.21 -7.68 -1.50 -1.30 7.49 4.97 -0.35 7.16 1.99 6.73 5.05 -3.51 7.00 1.71 9.51
Endosome -2.54 4.35 6.19 6.97 7.40 -3.87 20.35 19.52 18.72 0.36 -4.55 16.85 21.75 20.59 6.21
Golgi -1.70 4.37 3.58 3.59 -2.11 -6.38 15.44 4.99 9.11 -2.47 -6.58 15.81 8.77 12.54 -3.26
Lipid Particles 1.53 -0.24 5.51 4.77 6.09 8.60 14.68 17.09 8.41 4.60 8.82 12.75 17.30 8.76 7.29
Mitochondria -0.57 2.98 1.82 1.73 -2.71 -1.79 9.56 8.02 7.55 -1.83 -1.70 9.68 8.37 7.75 -3.25
Mitotic Spindle -1.71 -2.09 -7.03 0.10 -5.77 -3.58 -1.95 -3.96 -2.04 -3.32 -3.67 -2.42 -7.92 -4.31 -6.74
None -0.13 -2.64 -7.24 -6.71 -4.24 2.94 0.38 2.29 -1.54 1.16 2.98 1.51 1.43 -3.79 -0.52
Nuclear Periphery -0.80 -10.38 -19.59 -17.24 5.40 3.90 -3.08 2.95 -2.63 7.84 3.94 -3.94 1.52 -5.74 9.04
Nuclear Periphery Foci -1.14 -7.74 -7.27 -4.10 7.01 3.55 -5.98 -5.56 -6.80 4.54 3.24 -8.99 -7.98 -9.48 7.99
Nucleolus -0.42 -1.80 -0.69 0.12 1.50 -0.34 8.80 9.13 2.63 0.84 -0.29 7.39 8.86 2.56 1.59
Nucleus 2.17 -9.62 -20.76 -22.49 3.02 10.26 -7.09 -6.47 -14.46 5.41 10.55 -9.66 -11.73 -23.68 6.02
Peroxisomes -2.56 6.29 5.57 3.48 -1.08 -7.45 9.12 5.52 11.57 -3.11 -7.78 9.33 7.02 12.37 -2.92
Vacuole -0.45 -10.78 -12.58 -12.57 2.92 2.78 -13.74 -22.53 -23.85 4.53 2.48 -18.51 -31.04 -32.69 5.40
Vacuole Periphery -1.17 4.12 3.47 2.89 -1.26 -0.43 16.57 9.72 7.83 -2.13 -0.54 16.44 12.66 9.35 -2.54
Standard name
Human Ortholog
Description Subunit of the CCR4-NOT transcriptional regulatory complex; CCR4-NOT complex is evolutionarily-conserved and involved in controlling mRNA initiation, elongation, and degradation
Localization
Cell Percentages cytoplasm (78%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Caf130

Caf130


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Caf130-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available