Standard name
Human Ortholog
Description GTP-binding protein of the Ras superfamily; required for bud site selection, morphological changes in response to mating pheromone, and efficient cell fusion; localized to the plasma membrane; significantly similar to mammalian Rap GTPases
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.87 0.99 0.98 0.99 0.93 0.74 0.64 0.58 0.55 0.53 0.49 0.47 0.96 0.99 0.97 0.93 0.94 0.95 0.89 0.92 0.96 0.92 0.91 0.9
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.05 0 0 0 0 0.16 0.26 0.23 0.4 0.27 0.42 0.46 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0.1 0 0 0 0 0 0.05 0.07 0.11 0.12 0.1 0.09 0 0 0 0 0.05 0.05 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0.15 0.18 0.25 0.09 0.21 0.14 0.16 0 0 0 0.05 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 1 0 0 0
Bud 0 0 2 0 0 8 7 6 4 1 16 24 0 0 0 0 0 0 1 3 2 1 2 2
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 2 0 0 3 3 0 0 1 0 1 4 0 0 2 0 2 3 0 0 0 0 0 0
Cytoplasm 34 152 318 288 305 394 392 334 271 313 288 372 78 192 189 215 164 155 59 145 313 91 140 176
Endoplasmic Reticulum 0 0 3 0 2 3 2 4 11 9 11 13 3 0 2 5 5 5 0 0 0 0 0 2
Endosome 0 0 0 0 1 4 2 9 0 7 1 4 1 1 0 2 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 1
Mitochondria 2 0 1 3 6 83 156 130 200 159 248 367 0 0 0 3 0 0 0 0 1 0 0 1
Nucleus 4 2 1 1 6 24 31 42 57 69 57 69 0 1 3 3 8 8 1 3 0 2 1 2
Nuclear Periphery 0 0 0 0 1 0 5 0 1 0 5 2 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 1 0 1 0 2 1 1 0 1 1 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 2 4 14 81 111 144 47 122 84 127 0 1 2 11 4 3 0 0 0 2 5 3
Unique Cell Count 39 154 324 291 327 535 609 574 496 590 592 798 81 194 195 230 174 164 67 158 326 100 155 196
Labelled Cell Count 40 156 327 296 338 601 706 670 592 682 713 983 82 196 199 240 183 174 67 158 326 100 155 196


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 6.6 9.3 6.1 6.1 6.0 5.8 5.1 5.4 5.2 5.4 5.2 5.3 8.5 8.8 8.6 11.5 12.0 12.1 9.0 8.8 9.3
Std Deviation (1e-4) 1.6 2.0 1.1 1.3 1.1 0.9 1.0 1.0 0.8 0.9 0.9 0.9 1.6 1.8 1.7 2.5 2.4 2.4 1.4 1.5 1.8
Intensity Change (Log2) – – – -0.01 -0.04 -0.08 -0.28 -0.18 -0.24 -0.18 -0.25 -0.22 0.47 0.53 0.5 0.91 0.97 0.98 0.56 0.52 0.6


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 2.2 2.4 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.8 -3.1 -9.2 -11.5 -12.9 -13.5 -14.1 -15.1 -16.1 -1.0 0.7 -0.9 -2.8 -2.4 -2.2
Endoplasmic Reticulum 0 0 0 0 0 1.4 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 7.3 9.8 9.1 13.0 10.1 13.5 14.8 0 0 0 0 0 0
Nucleus 0 0 3.5 3.8 4.7 6.1 6.2 5.5 5.2 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 3.0 7.0 7.8 9.5 5.2 8.5 6.7 7.3 0 0 0 3.2 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 2.6791 5.431 5.0962 4.277 4.1808 4.34 4.7726 8.7136 7.5209 6.1524 6.2406 6.8451 2.508 6.2561 5.3024 4.4224 4.3594 4.3987
Actin 0.0094 0.0011 0.0033 0.0045 0.0003 0.0023 0.0026 0.0013 0.0021 0.0001 0.0003 0.0017 0.0034 0.0002 0.0019 0.0003 0.0001 0.0001
Bud 0.0003 0.0003 0.0011 0.0002 0.0001 0.0002 0.0045 0.0006 0.0072 0.0001 0.0018 0.0005 0.0002 0.0001 0.0001 0.0001 0.0001 0.0001
Bud Neck 0.0008 0.0003 0.0005 0.0005 0.0004 0.0012 0.0005 0.0002 0.0004 0.0004 0.0006 0.0017 0.0012 0.0001 0.0003 0.0004 0.0004 0.0008
Bud Periphery 0.0002 0.0001 0.0006 0 0 0.0001 0.0025 0.0005 0.0032 0 0.0006 0.0002 0.0001 0 0.0001 0 0 0
Bud Site 0.0007 0.0087 0.0023 0.0003 0.0002 0.0003 0.006 0.0011 0.0044 0.0003 0.0003 0.0003 0.0004 0.0003 0.0003 0.0001 0.0001 0.0001
Cell Periphery 0.0001 0 0.0001 0 0 0 0.0002 0.0001 0.0001 0 0 0 0.0001 0 0.0001 0 0 0
Cytoplasm 0.802 0.9187 0.884 0.9023 0.8959 0.9183 0.897 0.9527 0.9387 0.9278 0.92 0.9249 0.8873 0.9744 0.9554 0.9193 0.9427 0.9497
Cytoplasmic Foci 0.018 0.0096 0.0076 0.0119 0.0068 0.0103 0.0077 0.0032 0.0043 0.0082 0.0051 0.007 0.0143 0.0024 0.0029 0.0031 0.0026 0.0038
Eisosomes 0.0001 0 0.0001 0 0 0 0 0 0 0 0 0 0.0001 0 0.0008 0 0 0
Endoplasmic Reticulum 0.0075 0.0021 0.0027 0.0023 0.0035 0.0045 0.0051 0.0008 0.0016 0.0009 0.0024 0.0022 0.0062 0.0011 0.0016 0.001 0.0011 0.0013
Endosome 0.0156 0.0058 0.0094 0.0046 0.0421 0.0081 0.0038 0.0009 0.0017 0.0025 0.0045 0.0046 0.0085 0.0008 0.0017 0.0014 0.001 0.0015
Golgi 0.0016 0.0004 0.0006 0.0005 0.0025 0.0016 0.0003 0.0001 0.0004 0.0001 0.0001 0.0003 0.0013 0.0001 0.0002 0 0 0
Lipid Particles 0.0013 0.0001 0.0004 0.0007 0.0039 0.0002 0.0002 0 0.0002 0 0.0001 0.0002 0.002 0 0.0002 0 0 0
Mitochondria 0.0007 0.0002 0.0005 0.0004 0.0006 0.0005 0.0003 0.0001 0.0003 0.0001 0.0002 0.0002 0.0014 0.0001 0.0019 0.0002 0.0001 0.0001
None 0.1056 0.0229 0.052 0.0161 0.0132 0.0276 0.0546 0.0095 0.0165 0.04 0.035 0.0336 0.0456 0.0075 0.0065 0.0502 0.0069 0.0119
Nuclear Periphery 0.0067 0.0024 0.0028 0.0031 0.0029 0.0026 0.0024 0.001 0.0014 0.001 0.0023 0.0021 0.0054 0.001 0.0017 0.0018 0.0035 0.0019
Nucleolus 0.0006 0.0001 0.0002 0.0001 0.0001 0.0001 0.0001 0 0.0001 0.0002 0.0001 0.0001 0.0001 0 0 0 0 0
Nucleus 0.016 0.024 0.0258 0.027 0.0232 0.0174 0.0087 0.0261 0.0143 0.0121 0.0231 0.0164 0.014 0.0109 0.0229 0.0204 0.0394 0.0265
Peroxisomes 0.0012 0.0003 0.0017 0.0215 0.0005 0.0003 0.0003 0 0.001 0.0001 0.0001 0.0002 0.003 0 0.0002 0.0001 0 0
Punctate Nuclear 0.0068 0.0017 0.0018 0.0025 0.0015 0.0025 0.0019 0.0012 0.0015 0.0051 0.0019 0.0029 0.003 0.0005 0.0006 0.0009 0.0009 0.0014
Vacuole 0.0041 0.0011 0.0019 0.0013 0.0016 0.0014 0.0014 0.0003 0.0006 0.001 0.0013 0.0007 0.0019 0.0003 0.0006 0.0006 0.0008 0.0006
Vacuole Periphery 0.0007 0.0002 0.0003 0.0002 0.0005 0.0003 0.0002 0 0.0001 0.0001 0.0002 0.0001 0.0004 0.0001 0.0001 0.0001 0.0001 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 40.4177 10.8393 8.0432 27.2944 38.4291 33.6628 12.1773 12.7393 15.8737 39.8246
Translational Efficiency 2.7408 2.2259 2.5838 1.3261 2.0195 3.1977 2.0646 1.3224 1.6859 1.8821

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
2005 1952 1691 1075 1695 1809 221 1628 3700 3761 1912 2703

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 837.30 946.16 1123.65 1201.05 868.49 868.71 1321.26 1215.42 851.59 908.91 1146.49 1209.70
Standard Deviation 130.51 152.59 177.43 212.95 130.54 142.21 157.35 216.47 131.45 152.67 186.27 215.19
Intensity Change Log 2 — 0.176340 0.424376 0.520480 — 0.000365 0.605333 0.484874 — 0.089426 0.519341 0.502461

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000170 0.000168 0.000228 0.005134 0.000126 0.000226 0.001294 0.003677 0.000150 0.000196 0.000351 0.004257
Bud Neck 0.003509 0.002881 0.002034 0.005283 0.003053 0.005486 0.002276 0.004822 0.003300 0.004134 0.002062 0.005005
Bud Site 0.006427 0.006002 0.004763 0.040104 0.004575 0.011122 0.008989 0.029884 0.005578 0.008465 0.005251 0.033949
Cell Periphery 0.000090 0.000057 0.000109 0.000252 0.000078 0.000087 0.000277 0.000110 0.000085 0.000071 0.000129 0.000167
Cytoplasm 0.739539* 0.779877* 0.600562* 0.545265* 0.760767* 0.730013* 0.439366* 0.708285* 0.749263* 0.755893* 0.581930* 0.643451*
Cytoplasmic Foci 0.070965 0.048108 0.005035 0.025599 0.072433 0.095790 0.012926 0.011061 0.071638 0.071042 0.005947 0.016843
Eisosomes 0.000029 0.000015 0.000012 0.000027 0.000023 0.000037 0.000063 0.000013 0.000026 0.000026 0.000018 0.000019
Endoplasmic Reticulum 0.000743 0.000611 0.001525 0.001281 0.000852 0.000693 0.004729 0.000595 0.000793 0.000650 0.001896 0.000868
Endosome 0.003642 0.003048 0.001116 0.014388 0.003493 0.008063 0.006899 0.003940 0.003574 0.005460 0.001784 0.008095
Golgi 0.000964 0.000996 0.000098 0.016183 0.000545 0.002003 0.000375 0.006865 0.000772 0.001480 0.000130 0.010570
Lipid Particles 0.001241 0.000699 0.000520 0.001056 0.000880 0.001492 0.003218 0.000996 0.001075 0.001081 0.000832 0.001020
Mitochondria 0.002888 0.000719 0.000318 0.003679 0.000958 0.001680 0.000323 0.001701 0.002003 0.001181 0.000319 0.002488
Mitotic Spindle 0.007843 0.004480 0.005200 0.085535 0.001332 0.006604 0.007881 0.040393 0.004860 0.005502 0.005510 0.058347
None 0.010138 0.007471 0.016838 0.028464 0.008698 0.004565 0.003922 0.023906 0.009478 0.006073 0.015345 0.025719
Nuclear Periphery 0.000240 0.000209 0.000717 0.000605 0.000170 0.000171 0.001374 0.000351 0.000208 0.000191 0.000793 0.000452
Nuclear Periphery Foci 0.001223 0.001738 0.002221 0.004285 0.001535 0.001131 0.014134 0.001092 0.001366 0.001446 0.003598 0.002362
Nucleolus 0.000892 0.000666 0.000250 0.000547 0.000376 0.000830 0.000199 0.000235 0.000656 0.000745 0.000244 0.000359
Nucleus 0.104313 0.096663 0.319041* 0.151408* 0.094371 0.066104 0.215190* 0.118476 0.099758 0.081964 0.307037* 0.131573*
Peroxisomes 0.000738 0.001122 0.000178 0.004243 0.000670 0.002311 0.005973 0.000978 0.000707 0.001694 0.000848 0.002277
Vacuole 0.043830 0.043894 0.039133 0.060098 0.044556 0.060598 0.270361* 0.040942 0.044163 0.051928 0.065859 0.048560
Vacuole Periphery 0.000574 0.000574 0.000103 0.006564 0.000510 0.000994 0.000229 0.001676 0.000545 0.000776 0.000117 0.003620

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 0.07 -3.77 -4.40 -4.42 -3.97 -2.41 -4.81 -2.02 -1.81 -0.54 -1.58 -7.08 -3.68 -3.54 -2.96
Bud Neck 1.48 0.55 -1.35 -2.80 -1.62 -4.09 -0.71 -0.52 3.28 0.08 -2.31 0.07 -0.87 1.28 -0.73
Bud Site 0.52 1.08 -4.79 -5.34 -5.53 -5.36 -3.33 -4.39 -0.18 -2.11 -3.55 0.08 -6.06 -3.59 -5.74
Cell Periphery 6.71 -5.27 -7.32 -10.36 -4.10 -1.22 -8.51 -1.77 -0.33 8.02 3.33 -8.52 -6.69 -9.15 1.70
Cytoplasm -4.11 27.84 28.01 31.39 4.68 4.46 24.59 22.31 16.68 -13.18 0.25 36.43 34.03 33.03 -4.71
Cytoplasmic Foci 7.07 26.77 23.10 16.85 -3.76 -5.48 22.56 23.83 27.73 2.17 0.26 35.85 33.85 32.20 -3.21
Eisosomes 8.39 7.30 2.33 -5.35 -4.23 -5.30 -6.82 4.29 8.16 8.58 0.27 3.64 5.54 4.21 1.56
Endoplasmic Reticulum 0.93 -8.73 -3.03 -5.48 7.33 1.05 -7.68 1.73 0.98 8.91 1.39 -10.70 -0.20 -2.55 12.49
Endosome 1.73 7.13 0.61 -0.32 -2.46 -6.17 -0.74 6.20 9.69 5.12 -4.12 8.25 4.07 6.85 -1.06
Golgi 0.10 3.62 -1.93 -1.95 -2.77 -3.76 2.73 -1.67 0.76 -2.24 -2.56 4.72 -2.41 -1.17 -3.69
Lipid Particles 3.83 3.47 -2.82 -7.48 -7.77 -3.66 -6.33 -0.37 2.84 5.65 0.07 1.00 -1.36 -1.36 -2.41
Mitochondria 2.53 2.89 1.90 -1.67 -2.99 -0.98 1.67 0.81 1.57 -1.73 1.48 3.24 2.24 0.83 -3.24
Mitotic Spindle 1.76 0.60 -7.15 -8.19 -7.66 -3.42 -3.40 -9.68 -7.04 -5.83 -0.52 -1.52 -11.16 -10.96 -10.24
None 2.95 -1.96 -2.40 -4.57 -0.82 4.18 4.61 -4.85 -10.24 -10.35 5.04 -2.08 -5.07 -10.06 -2.40
Nuclear Periphery 0.16 -15.68 -17.03 -21.29 -5.73 -0.13 -13.93 -19.90 -19.51 7.30 0.16 -24.48 -23.47 -28.34 0.46
Nuclear Periphery Foci -1.91 -6.44 -3.19 -2.03 -0.10 2.31 -6.93 1.68 -0.93 7.21 -0.47 -8.50 -2.44 -1.87 4.92
Nucleolus 0.92 2.65 2.40 2.40 -0.69 -1.98 3.60 2.45 2.56 -1.59 -0.43 2.98 2.90 3.56 -0.29
Nucleus 0.40 -41.21 -18.27 -18.53 17.98 7.93 -11.47 -21.16 -27.71 4.20 5.50 -44.33 -27.63 -31.66 22.97
Peroxisomes -1.57 5.27 -0.74 0.31 -2.78 -4.32 -0.60 2.60 5.59 0.92 -4.49 2.33 1.12 4.74 -1.18
Vacuole -1.88 -7.93 -20.96 -20.07 -15.72 -5.77 -14.99 -18.76 -14.65 9.08 -5.48 -13.54 -27.95 -24.75 -11.20
Vacuole Periphery 0.25 4.58 -1.35 -1.42 -2.30 -1.90 2.60 0.51 2.06 -1.16 -1.41 5.35 -0.82 0.01 -2.72
Standard name
Human Ortholog
Description GTP-binding protein of the Ras superfamily; required for bud site selection, morphological changes in response to mating pheromone, and efficient cell fusion; localized to the plasma membrane; significantly similar to mammalian Rap GTPases
Localization
Cell Percentages cytoplasm (97%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Rsr1

Rsr1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Rsr1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available