Standard name | |
---|---|
Human Ortholog | |
Description | GTP-binding protein of the Ras superfamily; required for bud site selection, morphological changes in response to mating pheromone, and efficient cell fusion; localized to the plasma membrane; significantly similar to mammalian Rap GTPases |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 |
Bud | 0 | 0 | 2 | 0 | 0 | 8 | 7 | 6 | 4 | 1 | 16 | 24 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 3 | 2 | 1 | 2 | 2 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 2 | 0 | 0 | 3 | 3 | 0 | 0 | 1 | 0 | 1 | 4 | 0 | 0 | 2 | 0 | 2 | 3 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 34 | 152 | 318 | 288 | 305 | 394 | 392 | 334 | 271 | 313 | 288 | 372 | 78 | 192 | 189 | 215 | 164 | 155 | 59 | 145 | 313 | 91 | 140 | 176 |
Endoplasmic Reticulum | 0 | 0 | 3 | 0 | 2 | 3 | 2 | 4 | 11 | 9 | 11 | 13 | 3 | 0 | 2 | 5 | 5 | 5 | 0 | 0 | 0 | 0 | 0 | 2 |
Endosome | 0 | 0 | 0 | 0 | 1 | 4 | 2 | 9 | 0 | 7 | 1 | 4 | 1 | 1 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
Mitochondria | 2 | 0 | 1 | 3 | 6 | 83 | 156 | 130 | 200 | 159 | 248 | 367 | 0 | 0 | 0 | 3 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 |
Nucleus | 4 | 2 | 1 | 1 | 6 | 24 | 31 | 42 | 57 | 69 | 57 | 69 | 0 | 1 | 3 | 3 | 8 | 8 | 1 | 3 | 0 | 2 | 1 | 2 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 1 | 0 | 5 | 0 | 1 | 0 | 5 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 2 | 1 | 1 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 0 | 0 | 2 | 4 | 14 | 81 | 111 | 144 | 47 | 122 | 84 | 127 | 0 | 1 | 2 | 11 | 4 | 3 | 0 | 0 | 0 | 2 | 5 | 3 |
Unique Cell Count | 39 | 154 | 324 | 291 | 327 | 535 | 609 | 574 | 496 | 590 | 592 | 798 | 81 | 194 | 195 | 230 | 174 | 164 | 67 | 158 | 326 | 100 | 155 | 196 |
Labelled Cell Count | 40 | 156 | 327 | 296 | 338 | 601 | 706 | 670 | 592 | 682 | 713 | 983 | 82 | 196 | 199 | 240 | 183 | 174 | 67 | 158 | 326 | 100 | 155 | 196 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 6.6 | 9.3 | 6.1 | 6.1 | 6.0 | 5.8 | 5.1 | 5.4 | 5.2 | 5.4 | 5.2 | 5.3 | 8.5 | 8.8 | 8.6 | 11.5 | 12.0 | 12.1 | 9.0 | 8.8 | 9.3 |
Std Deviation (1e-4) | 1.6 | 2.0 | 1.1 | 1.3 | 1.1 | 0.9 | 1.0 | 1.0 | 0.8 | 0.9 | 0.9 | 0.9 | 1.6 | 1.8 | 1.7 | 2.5 | 2.4 | 2.4 | 1.4 | 1.5 | 1.8 |
Intensity Change (Log2) | – | – | – | -0.01 | -0.04 | -0.08 | -0.28 | -0.18 | -0.24 | -0.18 | -0.25 | -0.22 | 0.47 | 0.53 | 0.5 | 0.91 | 0.97 | 0.98 | 0.56 | 0.52 | 0.6 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2.2 | 2.4 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 0.8 | -3.1 | -9.2 | -11.5 | -12.9 | -13.5 | -14.1 | -15.1 | -16.1 | -1.0 | 0.7 | -0.9 | -2.8 | -2.4 | -2.2 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 1.4 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 7.3 | 9.8 | 9.1 | 13.0 | 10.1 | 13.5 | 14.8 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 3.5 | 3.8 | 4.7 | 6.1 | 6.2 | 5.5 | 5.2 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 3.0 | 7.0 | 7.8 | 9.5 | 5.2 | 8.5 | 6.7 | 7.3 | 0 | 0 | 0 | 3.2 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 2.6791 | 5.431 | 5.0962 | 4.277 | 4.1808 | 4.34 | 4.7726 | 8.7136 | 7.5209 | 6.1524 | 6.2406 | 6.8451 | 2.508 | 6.2561 | 5.3024 | 4.4224 | 4.3594 | 4.3987 |
Actin | 0.0094 | 0.0011 | 0.0033 | 0.0045 | 0.0003 | 0.0023 | 0.0026 | 0.0013 | 0.0021 | 0.0001 | 0.0003 | 0.0017 | 0.0034 | 0.0002 | 0.0019 | 0.0003 | 0.0001 | 0.0001 |
Bud | 0.0003 | 0.0003 | 0.0011 | 0.0002 | 0.0001 | 0.0002 | 0.0045 | 0.0006 | 0.0072 | 0.0001 | 0.0018 | 0.0005 | 0.0002 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0001 |
Bud Neck | 0.0008 | 0.0003 | 0.0005 | 0.0005 | 0.0004 | 0.0012 | 0.0005 | 0.0002 | 0.0004 | 0.0004 | 0.0006 | 0.0017 | 0.0012 | 0.0001 | 0.0003 | 0.0004 | 0.0004 | 0.0008 |
Bud Periphery | 0.0002 | 0.0001 | 0.0006 | 0 | 0 | 0.0001 | 0.0025 | 0.0005 | 0.0032 | 0 | 0.0006 | 0.0002 | 0.0001 | 0 | 0.0001 | 0 | 0 | 0 |
Bud Site | 0.0007 | 0.0087 | 0.0023 | 0.0003 | 0.0002 | 0.0003 | 0.006 | 0.0011 | 0.0044 | 0.0003 | 0.0003 | 0.0003 | 0.0004 | 0.0003 | 0.0003 | 0.0001 | 0.0001 | 0.0001 |
Cell Periphery | 0.0001 | 0 | 0.0001 | 0 | 0 | 0 | 0.0002 | 0.0001 | 0.0001 | 0 | 0 | 0 | 0.0001 | 0 | 0.0001 | 0 | 0 | 0 |
Cytoplasm | 0.802 | 0.9187 | 0.884 | 0.9023 | 0.8959 | 0.9183 | 0.897 | 0.9527 | 0.9387 | 0.9278 | 0.92 | 0.9249 | 0.8873 | 0.9744 | 0.9554 | 0.9193 | 0.9427 | 0.9497 |
Cytoplasmic Foci | 0.018 | 0.0096 | 0.0076 | 0.0119 | 0.0068 | 0.0103 | 0.0077 | 0.0032 | 0.0043 | 0.0082 | 0.0051 | 0.007 | 0.0143 | 0.0024 | 0.0029 | 0.0031 | 0.0026 | 0.0038 |
Eisosomes | 0.0001 | 0 | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0.0001 | 0 | 0.0008 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0.0075 | 0.0021 | 0.0027 | 0.0023 | 0.0035 | 0.0045 | 0.0051 | 0.0008 | 0.0016 | 0.0009 | 0.0024 | 0.0022 | 0.0062 | 0.0011 | 0.0016 | 0.001 | 0.0011 | 0.0013 |
Endosome | 0.0156 | 0.0058 | 0.0094 | 0.0046 | 0.0421 | 0.0081 | 0.0038 | 0.0009 | 0.0017 | 0.0025 | 0.0045 | 0.0046 | 0.0085 | 0.0008 | 0.0017 | 0.0014 | 0.001 | 0.0015 |
Golgi | 0.0016 | 0.0004 | 0.0006 | 0.0005 | 0.0025 | 0.0016 | 0.0003 | 0.0001 | 0.0004 | 0.0001 | 0.0001 | 0.0003 | 0.0013 | 0.0001 | 0.0002 | 0 | 0 | 0 |
Lipid Particles | 0.0013 | 0.0001 | 0.0004 | 0.0007 | 0.0039 | 0.0002 | 0.0002 | 0 | 0.0002 | 0 | 0.0001 | 0.0002 | 0.002 | 0 | 0.0002 | 0 | 0 | 0 |
Mitochondria | 0.0007 | 0.0002 | 0.0005 | 0.0004 | 0.0006 | 0.0005 | 0.0003 | 0.0001 | 0.0003 | 0.0001 | 0.0002 | 0.0002 | 0.0014 | 0.0001 | 0.0019 | 0.0002 | 0.0001 | 0.0001 |
None | 0.1056 | 0.0229 | 0.052 | 0.0161 | 0.0132 | 0.0276 | 0.0546 | 0.0095 | 0.0165 | 0.04 | 0.035 | 0.0336 | 0.0456 | 0.0075 | 0.0065 | 0.0502 | 0.0069 | 0.0119 |
Nuclear Periphery | 0.0067 | 0.0024 | 0.0028 | 0.0031 | 0.0029 | 0.0026 | 0.0024 | 0.001 | 0.0014 | 0.001 | 0.0023 | 0.0021 | 0.0054 | 0.001 | 0.0017 | 0.0018 | 0.0035 | 0.0019 |
Nucleolus | 0.0006 | 0.0001 | 0.0002 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0 | 0.0001 | 0.0002 | 0.0001 | 0.0001 | 0.0001 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0.016 | 0.024 | 0.0258 | 0.027 | 0.0232 | 0.0174 | 0.0087 | 0.0261 | 0.0143 | 0.0121 | 0.0231 | 0.0164 | 0.014 | 0.0109 | 0.0229 | 0.0204 | 0.0394 | 0.0265 |
Peroxisomes | 0.0012 | 0.0003 | 0.0017 | 0.0215 | 0.0005 | 0.0003 | 0.0003 | 0 | 0.001 | 0.0001 | 0.0001 | 0.0002 | 0.003 | 0 | 0.0002 | 0.0001 | 0 | 0 |
Punctate Nuclear | 0.0068 | 0.0017 | 0.0018 | 0.0025 | 0.0015 | 0.0025 | 0.0019 | 0.0012 | 0.0015 | 0.0051 | 0.0019 | 0.0029 | 0.003 | 0.0005 | 0.0006 | 0.0009 | 0.0009 | 0.0014 |
Vacuole | 0.0041 | 0.0011 | 0.0019 | 0.0013 | 0.0016 | 0.0014 | 0.0014 | 0.0003 | 0.0006 | 0.001 | 0.0013 | 0.0007 | 0.0019 | 0.0003 | 0.0006 | 0.0006 | 0.0008 | 0.0006 |
Vacuole Periphery | 0.0007 | 0.0002 | 0.0003 | 0.0002 | 0.0005 | 0.0003 | 0.0002 | 0 | 0.0001 | 0.0001 | 0.0002 | 0.0001 | 0.0004 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0001 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 40.4177 | 10.8393 | 8.0432 | 27.2944 | 38.4291 | 33.6628 | 12.1773 | 12.7393 | 15.8737 | 39.8246 |
Translational Efficiency | 2.7408 | 2.2259 | 2.5838 | 1.3261 | 2.0195 | 3.1977 | 2.0646 | 1.3224 | 1.6859 | 1.8821 |
Dataset | Hit |
---|---|
Protein Concentration | ✔ |
Protein Localization | ✘ |
Gene Expression | ✔ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
---|
Gene | Images |
---|