Standard name
Human Ortholog
Description Essential component of CPF (cleavage and polyadenylation factor); involved in 3' end formation of snoRNA and mRNA; interacts directly with Pta1p; relocalizes to the cytosol in response to hypoxia; similar to mammalian Cleavage-Stimulation Factor CstF-64
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0.06 0.07 0 0 0 0.05 0.05 0.05 0 0 0.12 0.14 0.16 0.07 0.05 0.07 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0.06 0.05 0 0.11 0.13 0.1 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0.94 0.97 0.9 0.87 0.89 0.86 0.79 0.79 0.77 0.69 0.7 0.71 0.87 0.83 0.78 0.89 0.85 0.84 0.92 0.92 0.88 0.81 0.83 0.72
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.13 0.16 0.06 0.12 0.18 0.26 0.31 0.26 0.35 0.43 0.47 0.46 0.07 0 0.07 0 0.07 0.08 0 0 0 0 0.05 0.1
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.05 0.11
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 3 1 1 0 3 1 0 1 0 1 0 0 0 1 0 1 3 5
Bud Neck 0 0 1 0 0 0 4 0 3 1 5 3 0 0 0 0 0 0 0 0 0 0 0 1
Bud Site 0 0 0 0 2 5 6 6 2 6 11 8 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 2 3 9 10 8 12 16 19 14 18 11 9 29 56 75 17 16 21 1 0 0 1 1 0
Endoplasmic Reticulum 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 3 1 4 0 0 0 0 0 1
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 2 3 0 4 4 2 0 0 3 3 1
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 3 1 1 0 0 2 1 0
Mitochondria 9 1 2 0 1 13 25 17 10 42 40 34 0 0 1 1 13 3 0 0 3 1 1 5
Nucleus 224 123 137 117 172 263 323 275 222 260 221 240 216 333 369 203 283 254 218 125 138 151 244 247
Nuclear Periphery 2 0 0 1 1 4 4 2 1 2 3 3 1 2 2 0 5 2 0 0 0 1 0 0
Nucleolus 30 20 9 16 35 79 129 89 101 163 148 156 17 11 32 9 23 24 8 3 6 8 15 33
Peroxisomes 0 0 0 0 0 0 0 0 0 0 1 0 0 0 3 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 1 5 13 1 2 0 1 0 1 1 3 6
Vac/Vac Membrane 3 0 2 1 1 2 3 9 2 8 5 4 0 2 4 5 10 7 2 4 2 10 13 37
Unique Cell Count 238 127 153 135 193 306 411 349 288 378 317 337 248 399 475 228 332 303 238 136 157 187 295 345
Labelled Cell Count 271 147 160 145 220 378 514 418 356 500 449 458 264 412 502 241 360 320 238 136 157 187 295 345


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 8.8 10.8 10.0 9.3 9.0 8.1 7.6 8.3 7.9 7.9 7.2 7.3 10.4 10.8 9.9 10.9 10.2 10.5 9.6 10.9 11.2
Std Deviation (1e-4) 1.9 2.9 3.8 2.6 3.3 3.1 2.7 3.5 3.8 3.9 3.1 2.9 2.8 3.0 3.0 2.7 2.4 2.4 2.1 2.1 2.1
Intensity Change (Log2) – – – -0.11 -0.16 -0.31 -0.39 -0.27 -0.34 -0.34 -0.47 -0.46 0.06 0.1 -0.02 0.13 0.03 0.07 -0.07 0.12 0.16


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.5 -0.7 -0.9 -1.0 -0.2 -0.5 -0.5 -1.2 -1.8 1.9 2.7 3.1 0.6 -0.5 0.4
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 1.7 2.4 1.9 0 3.7 4.0 3.5 0 0 0 0 0 0
Nucleus -0.8 -0.1 -1.1 -3.0 -2.9 -3.2 -5.0 -4.7 -4.5 -0.7 -1.8 -3.2 -0.2 -1.3 -1.6
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 1.8 3.4 5.1 6.3 5.1 6.7 8.3 8.8 8.8 0.4 -1.8 0.4 -0.9 0.4 0.8
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 8.6942 8.9067 8.1227 7.5094 7.1785 7.8324 5.0579 6.7707 6.3906 6.1166 5.8348 5.9215 6.8412 7.2815 6.1948 6.6719 5.6618 6.5539
Actin 0.0059 0 0.0001 0 0 0 0.0434 0.0006 0.0014 0 0.0401 0.0007 0.0017 0.0002 0.0008 0.0006 0.0004 0.0008
Bud 0.0002 0 0 0 0 0 0.0013 0.001 0.0006 0 0.0071 0.0001 0.0007 0 0.0002 0.0004 0.0003 0.0001
Bud Neck 0.0002 0 0 0 0.0001 0.0004 0.0013 0.0002 0.0002 0 0.0016 0.0005 0.0008 0 0.0014 0.0003 0.0009 0.0004
Bud Periphery 0.0002 0 0 0 0.0001 0 0.0016 0.0004 0.0011 0 0.0428 0.0003 0.0027 0 0.0003 0.0002 0.0003 0.0001
Bud Site 0.0012 0 0.0001 0 0.0001 0 0.008 0.013 0.0029 0 0.0019 0.0002 0.0024 0 0.0026 0.0008 0.0006 0.0001
Cell Periphery 0.0001 0 0 0 0 0 0.0005 0.0001 0.0004 0 0.0038 0.0001 0.0022 0 0.0002 0 0.0002 0
Cytoplasm 0.001 0 0.0004 0 0 0 0.0218 0.0026 0.0007 0 0.0025 0.0003 0.0067 0.0001 0.0023 0.0157 0.008 0.0009
Cytoplasmic Foci 0.0019 0 0.0057 0 0 0 0.0478 0.0015 0.0056 0 0.0108 0.0045 0.0191 0.0003 0.0143 0.0084 0.0108 0.0024
Eisosomes 0 0 0 0 0 0 0.0003 0 0.0001 0 0.0003 0 0.0001 0 0 0 0 0
Endoplasmic Reticulum 0.0002 0 0.0001 0 0 0 0.0066 0 0.0009 0 0.0087 0.0004 0.0015 0 0.0011 0.0007 0.003 0.0007
Endosome 0.0011 0 0.0003 0 0 0 0.0307 0.0005 0.0035 0 0.037 0.015 0.0152 0.0002 0.0093 0.0161 0.0378 0.0036
Golgi 0.0005 0 0.0001 0 0 0 0.0128 0.0004 0.0018 0 0.0154 0.0054 0.0045 0.0001 0.0022 0.003 0.0035 0.0035
Lipid Particles 0.0009 0 0.0005 0 0 0 0.0509 0.0004 0.0021 0 0.0481 0.012 0.0381 0.0001 0.0086 0.0002 0.0236 0.0007
Mitochondria 0.0008 0.0001 0.0001 0.0001 0.0008 0.0001 0.0098 0.0034 0.006 0 0.0141 0.0115 0.0141 0.0001 0.0022 0.001 0.0029 0.0005
None 0.0007 0 0.0006 0 0 0 0.0188 0 0.0009 0 0.0007 0.0001 0.0015 0 0.0007 0.0002 0.0017 0.0001
Nuclear Periphery 0.0021 0.0006 0.0008 0.0002 0.0015 0.0004 0.0285 0.0002 0.0038 0.0002 0.0139 0.0048 0.0067 0.0012 0.0043 0.0005 0.0104 0.0055
Nucleolus 0.0242 0.0175 0.0094 0.0088 0.1408 0.0909 0.0317 0.0168 0.0137 0.0079 0.1383 0.062 0.0365 0.013 0.0139 0.0656 0.2633 0.0665
Nucleus 0.9519 0.9795 0.9765 0.9909 0.8545 0.9079 0.6393 0.9577 0.9401 0.9918 0.5762 0.8751 0.8163 0.9745 0.9187 0.8841 0.6139 0.9062
Peroxisomes 0.0005 0 0.0001 0 0 0 0.0162 0.0002 0.001 0 0.0015 0.0008 0.0086 0.0001 0.0024 0.0008 0.0016 0.0002
Punctate Nuclear 0.006 0 0.0051 0.0001 0.0016 0.0002 0.0207 0.0001 0.011 0.0001 0.0148 0.0024 0.0102 0.0098 0.0105 0.0007 0.0117 0.0072
Vacuole 0.0004 0.0005 0 0 0.0001 0.0001 0.0044 0.0005 0.0014 0 0.0151 0.0023 0.0066 0 0.0022 0.0006 0.0035 0.0003
Vacuole Periphery 0.0002 0.0016 0 0 0.0002 0 0.0037 0.0004 0.0009 0 0.0054 0.0015 0.0035 0 0.0019 0.0002 0.0015 0.0002

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 20.2895 14.6644 12.5998 15.0093 13.433 12.0807 19.3144 19.2247 16.0837 20.4263
Translational Efficiency 1.4685 1.7462 1.5894 0.996 1.819 2.7199 1.3217 1.2908 1.4453 1.5075

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1597 1097 269 133 1887 1768 993 115 3484 2865 1262 248

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 972.31 984.88 1253.60 1325.48 964.10 1037.41 1205.63 1384.37 967.86 1017.30 1215.85 1352.79
Standard Deviation 171.40 219.48 154.50 223.57 162.10 165.42 201.75 223.38 166.48 189.69 193.65 225.41
Intensity Change Log 2 — 0.018532 0.366589 0.447027 — 0.105731 0.322533 0.521975 — 0.062605 0.344822 0.484829

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000426 0.000778 0.001122 0.000972 0.000429 0.000418 0.000835 0.000800 0.000427 0.000556 0.000896 0.000892
Bud Neck 0.003201 0.007951 0.011502 0.011696 0.005709 0.008336 0.010570 0.011538 0.004560 0.008188 0.010769 0.011623
Bud Site 0.000735 0.002158 0.001091 0.001555 0.000687 0.000727 0.000948 0.000754 0.000709 0.001275 0.000978 0.001184
Cell Periphery 0.000118 0.000181 0.000035 0.000118 0.000218 0.000076 0.000065 0.000032 0.000172 0.000116 0.000059 0.000078
Cytoplasm 0.001251 0.002955 0.000078 0.000906 0.000333 0.000098 0.000246 0.000723 0.000754 0.001192 0.000210 0.000821
Cytoplasmic Foci 0.000133 0.000702 0.000040 0.000107 0.000114 0.000217 0.000254 0.000023 0.000122 0.000403 0.000208 0.000068
Eisosomes 0.000073 0.000054 0.000084 0.000048 0.000092 0.000068 0.000070 0.000048 0.000083 0.000063 0.000073 0.000048
Endoplasmic Reticulum 0.002481 0.002485 0.001917 0.001628 0.002645 0.002441 0.001915 0.001664 0.002570 0.002458 0.001916 0.001645
Endosome 0.000110 0.000327 0.000048 0.000184 0.000132 0.000427 0.000152 0.000044 0.000122 0.000389 0.000130 0.000119
Golgi 0.000133 0.000277 0.000095 0.009150 0.000118 0.000199 0.000125 0.000055 0.000125 0.000229 0.000118 0.004933
Lipid Particles 0.000034 0.000435 0.000038 0.000025 0.000037 0.000915 0.000966 0.000030 0.000036 0.000731 0.000768 0.000028
Mitochondria 0.000640 0.001578 0.001571 0.003899 0.001128 0.000928 0.001291 0.001598 0.000904 0.001177 0.001350 0.002832
Mitotic Spindle 0.000695 0.001761 0.000163 0.003429 0.001120 0.001696 0.003013 0.000139 0.000925 0.001721 0.002406 0.001903
None 0.002221 0.005136 0.000848 0.004696 0.001577 0.000863 0.002369 0.000847 0.001872 0.002499 0.002045 0.002911
Nuclear Periphery 0.000044 0.000100 0.000093 0.000239 0.000059 0.000168 0.000106 0.000193 0.000052 0.000142 0.000103 0.000218
Nuclear Periphery Foci 0.000299 0.000411 0.000025 0.000056 0.000281 0.000295 0.000214 0.000015 0.000289 0.000339 0.000174 0.000037
Nucleolus 0.030295 0.030207 0.040694 0.014412 0.034297 0.030218 0.032036 0.007229 0.032462 0.030214 0.033881 0.011081
Nucleus 0.955702* 0.940146* 0.937258* 0.945052* 0.949764* 0.949684* 0.942642* 0.972887* 0.952486* 0.946032* 0.941495* 0.957960*
Peroxisomes 0.000434 0.000861 0.002555 0.000646 0.000604 0.000419 0.001219 0.000659 0.000526 0.000588 0.001504 0.000652
Vacuole 0.000695 0.001040 0.000592 0.000645 0.000468 0.001259 0.000832 0.000626 0.000572 0.001175 0.000781 0.000636
Vacuole Periphery 0.000281 0.000458 0.000151 0.000536 0.000189 0.000549 0.000131 0.000095 0.000231 0.000514 0.000135 0.000332

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -2.88 -10.87 -5.28 -1.93 0.61 0.41 -4.71 -4.08 -4.16 -1.60 -2.49 -6.73 -6.46 -5.02 -1.59
Bud Neck -7.44 -8.30 -7.83 -3.89 -0.84 -3.35 -5.71 -5.26 -3.95 -2.77 -6.83 -9.19 -8.60 -5.42 -3.00
Bud Site -2.08 -1.63 -1.82 0.57 -1.13 -0.33 -1.99 -1.68 -1.64 -1.42 -2.02 -1.94 -2.38 -1.33 -1.88
Cell Periphery -1.48 3.39 0.46 1.73 -2.11 2.32 2.49 2.56 0.78 0.16 1.50 3.15 2.34 1.42 -1.16
Cytoplasm -1.31 2.24 0.88 1.91 -2.61 2.59 0.46 -1.19 -2.00 -1.37 -0.80 2.07 0.13 0.94 -2.45
Cytoplasmic Foci -2.13 1.26 0.41 2.30 -1.11 -0.82 -1.10 0.73 1.44 1.63 -2.24 -0.86 0.74 2.65 1.36
Eisosomes 2.12 -1.60 2.31 0.32 5.44 2.71 3.74 5.51 1.48 3.03 3.13 1.67 5.41 1.57 5.30
Endoplasmic Reticulum -0.01 2.06 1.66 1.68 0.47 1.15 3.44 1.09 0.55 -0.74 0.87 4.01 2.02 1.64 -0.13
Endosome -2.43 2.35 -0.41 1.61 -1.24 -2.14 -0.33 0.78 2.49 0.94 -2.93 -0.19 -0.00 2.75 0.16
Golgi -1.33 0.52 -0.99 -0.97 -1.00 -0.66 -0.01 -0.07 0.77 -0.08 -1.25 0.18 -1.00 -0.96 -1.00
Lipid Particles -1.11 -0.41 -0.23 1.10 0.28 -1.61 -1.06 -1.14 1.54 1.02 -1.92 -1.06 -1.03 1.87 1.03
Mitochondria -2.27 -6.33 -2.56 -1.66 -1.71 0.39 -0.47 -1.89 -4.36 -3.23 -0.89 -1.84 -2.94 -2.61 -2.34
Mitotic Spindle -1.03 0.97 -0.95 -0.74 -1.05 -0.60 -1.17 -0.00 0.53 1.10 -1.13 -1.19 -0.92 -0.64 -0.44
None -2.64 2.86 -0.99 0.75 -1.96 2.34 -0.89 1.68 -0.83 1.53 -1.32 -0.15 -0.78 -0.04 -0.55
Nuclear Periphery -4.05 -4.70 -2.78 -2.26 -2.26 -2.99 -3.74 -5.61 -2.62 -4.45 -3.88 -5.27 -4.57 -2.93 -3.64
Nuclear Periphery Foci -0.47 1.90 1.37 2.40 -1.53 -0.05 0.23 1.57 2.39 1.33 -0.28 0.65 2.03 3.17 1.03
Nucleolus 0.00 -2.80 2.69 2.64 4.19 1.82 0.41 4.55 3.61 4.02 1.32 -1.20 5.14 4.38 5.32
Nucleus 3.65 4.35 1.46 0.01 -0.44 0.14 2.35 -0.42 -0.47 -1.67 2.58 4.11 1.03 0.01 -0.90
Peroxisomes -2.21 -6.35 -2.69 0.58 5.15 1.86 -4.16 -1.08 -2.27 1.81 -0.62 -7.85 -2.19 -1.50 4.73
Vacuole -0.82 0.36 -0.51 0.58 -1.39 -1.64 -1.44 -2.70 -0.54 -0.33 -1.77 -1.28 -2.69 -0.01 0.04
Vacuole Periphery -1.33 1.90 -0.46 0.21 -0.95 -2.14 1.81 1.57 2.41 -0.38 -2.43 2.59 -0.37 1.31 -1.04
Standard name
Human Ortholog
Description Essential component of CPF (cleavage and polyadenylation factor); involved in 3' end formation of snoRNA and mRNA; interacts directly with Pta1p; relocalizes to the cytosol in response to hypoxia; similar to mammalian Cleavage-Stimulation Factor CstF-64
Localization
Cell Percentages nucleus (100%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-4

Pti1

Pti1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Pti1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available