Standard name | |
---|---|
Human Ortholog | |
Description | Phosphatidylethanolamine methyltransferase (PEMT); catalyzes the first step in the conversion of phosphatidylethanolamine to phosphatidylcholine during the methylation pathway of phosphatidylcholine biosynthesis |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 42 | 51 | 94 | 60 | 42 | 85 | 88 | 50 | – | 78 | 74 | 93 | 14 | 18 | 18 | 163 | 84 | 53 | 14 | 10 | 24 | 21 | 29 | 18 |
Bud | 7 | 18 | 7 | 8 | 9 | 12 | 11 | 12 | – | 17 | 17 | 13 | 1 | 1 | 2 | 27 | 13 | 9 | 0 | 0 | 0 | 0 | 2 | 2 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 1 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 76 | 60 | 124 | 69 | 42 | 58 | 63 | 37 | – | 71 | 56 | 73 | 50 | 50 | 86 | 201 | 116 | 94 | 27 | 26 | 67 | 35 | 65 | 87 |
Cytoplasm | 50 | 38 | 22 | 16 | 23 | 53 | 43 | 46 | – | 78 | 66 | 86 | 15 | 15 | 32 | 121 | 90 | 57 | 7 | 16 | 1 | 1 | 2 | 6 |
Endoplasmic Reticulum | 333 | 215 | 334 | 182 | 224 | 322 | 435 | 401 | – | 280 | 178 | 201 | 160 | 161 | 221 | 15 | 7 | 9 | 387 | 281 | 347 | 206 | 263 | 354 |
Endosome | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 4 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 |
Golgi | 16 | 0 | 41 | 34 | 44 | 11 | 25 | 14 | – | 16 | 3 | 7 | 3 | 7 | 0 | 95 | 85 | 97 | 1 | 1 | 5 | 4 | 8 | 12 |
Mitochondria | 1 | 0 | 1 | 12 | 3 | 25 | 19 | 21 | – | 27 | 53 | 66 | 0 | 0 | 0 | 46 | 27 | 13 | 1 | 1 | 3 | 5 | 7 | 7 |
Nucleus | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 2 | – | 3 | 3 | 8 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 1 | – | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 3 | 0 | 0 | 0 | 0 | 0 | 1 | 0 |
Vac/Vac Membrane | 2 | 1 | 2 | 1 | 1 | 3 | 7 | 6 | – | 3 | 4 | 5 | 0 | 0 | 1 | 13 | 5 | 6 | 3 | 5 | 0 | 2 | 1 | 3 |
Unique Cell Count | 418 | 302 | 447 | 267 | 300 | 436 | 546 | 511 | 440 | 337 | 404 | 196 | 200 | 286 | 560 | 349 | 271 | 447 | 349 | 455 | 282 | 390 | 501 | |
Labelled Cell Count | 527 | 383 | 627 | 383 | 388 | 571 | 694 | 590 | 573 | 454 | 552 | 243 | 252 | 360 | 687 | 431 | 339 | 447 | 349 | 455 | 282 | 390 | 501 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 27.9 | 27.5 | 24.1 | 25.9 | 27.6 | 24.4 | 26.6 | 25.0 | – | 22.5 | 20.5 | 20.0 | 28.9 | 28.9 | 27.8 | 18.6 | 21.7 | 25.1 | 27.5 | 29.5 | 29.4 |
Std Deviation (1e-4) | 5.6 | 5.2 | 4.8 | 4.7 | 5.8 | 5.2 | 5.5 | 5.4 | – | 4.6 | 3.9 | 4.1 | 5.6 | 5.6 | 5.5 | 4.5 | 6.2 | 6.6 | 6.8 | 6.9 | 6.3 |
Intensity Change (Log2) | – | – | – | 0.1 | 0.19 | 0.02 | 0.14 | 0.05 | – | -0.1 | -0.24 | -0.27 | 0.26 | 0.26 | 0.21 | -0.37 | -0.15 | 0.06 | 0.19 | 0.29 | 0.29 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0.5 | -2.4 | -0.6 | -2.0 | -4.9 | – | -1.2 | 0.3 | 0.7 | -4.3 | -3.7 | -5.4 | 2.9 | 1.0 | -0.5 |
Bud | 1.3 | 1.3 | 1.2 | 0.5 | 0.9 | – | 2.1 | 2.8 | 1.6 | 0 | 0 | 0 | 2.8 | 1.9 | 1.5 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | -0.6 | -4.4 | -5.3 | -6.5 | -8.5 | – | -4.2 | -3.7 | -3.3 | -0.6 | -0.7 | 0.7 | 2.7 | 1.7 | 2.0 |
Cytoplasm | 0.6 | 1.5 | 3.9 | 1.9 | 2.5 | – | 6.0 | 6.4 | 7.2 | 1.4 | 1.3 | 3.2 | 7.5 | 8.4 | 6.7 |
Endoplasmic Reticulum | -1.9 | 0 | -0.3 | 1.9 | 1.4 | – | -3.6 | -6.4 | -7.5 | 1.9 | 1.6 | 0.8 | -23.9 | -20.6 | -18.6 |
Endosome | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 1.5 | 2.3 | -4.2 | -2.9 | -4.3 | – | -3.4 | -5.0 | -4.7 | -3.5 | -2.5 | -5.3 | 3.6 | 5.8 | 8.8 |
Mitochondria | 0 | 0 | 4.8 | 3.6 | 4.0 | – | 5.0 | 8.5 | 8.7 | 0 | 0 | 0 | 6.0 | 5.7 | 4.3 |
Nucleus | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 2.4 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 19.7366 | 23.8982 | 23.4688 | 23.5241 | 19.9786 | 23.5394 | 24.29 | 27.8141 | 27.1376 | 24.3686 | 26.0797 | 26.2353 | 31.0299 | 28.6985 | 29.9258 | 29.1608 | 26.2358 | 28.7331 |
Actin | 0.0619 | 0.0152 | 0.0497 | 0.009 | 0.0064 | 0.0131 | 0.0212 | 0.0048 | 0.0305 | 0.0053 | 0.017 | 0.01 | 0.0136 | 0.0054 | 0.0105 | 0.0052 | 0.0051 | 0.005 |
Bud | 0.0012 | 0.0029 | 0.0057 | 0.0021 | 0.0002 | 0.0002 | 0.0047 | 0.0003 | 0.0018 | 0.0002 | 0.0003 | 0.0005 | 0.0002 | 0.0003 | 0.0005 | 0.0004 | 0.0001 | 0.0001 |
Bud Neck | 0.004 | 0.0026 | 0.0058 | 0.004 | 0.0215 | 0.0123 | 0.0011 | 0.0017 | 0.0032 | 0.0019 | 0.0046 | 0.0078 | 0.001 | 0.0015 | 0.0022 | 0.0173 | 0.0025 | 0.0065 |
Bud Periphery | 0.004 | 0.0039 | 0.0224 | 0.0117 | 0.0015 | 0.0012 | 0.0077 | 0.0019 | 0.0135 | 0.0028 | 0.0044 | 0.0032 | 0.0009 | 0.0013 | 0.003 | 0.0034 | 0.0006 | 0.0012 |
Bud Site | 0.0063 | 0.0306 | 0.0158 | 0.0009 | 0.0002 | 0.0002 | 0.0032 | 0.0057 | 0.0018 | 0.0003 | 0.0002 | 0.0003 | 0.0005 | 0.0024 | 0.0022 | 0.0012 | 0 | 0.0001 |
Cell Periphery | 0.0607 | 0.0891 | 0.0765 | 0.0752 | 0.0214 | 0.027 | 0.0458 | 0.1091 | 0.0848 | 0.0492 | 0.0224 | 0.0282 | 0.0555 | 0.0749 | 0.0759 | 0.0731 | 0.0379 | 0.03 |
Cytoplasm | 0.0297 | 0.122 | 0.0572 | 0.0171 | 0.044 | 0.0271 | 0.0156 | 0.0447 | 0.0212 | 0.0058 | 0.0066 | 0.0128 | 0.0111 | 0.0311 | 0.0185 | 0.0123 | 0.0144 | 0.0083 |
Cytoplasmic Foci | 0.0153 | 0.0022 | 0.003 | 0.0022 | 0.0007 | 0.0013 | 0.0058 | 0.001 | 0.0015 | 0.0004 | 0.005 | 0.0013 | 0.004 | 0.001 | 0.0008 | 0.0011 | 0.0026 | 0.0007 |
Eisosomes | 0.0034 | 0.0035 | 0.0023 | 0.0016 | 0.0007 | 0.0033 | 0.0051 | 0.0085 | 0.0038 | 0.0049 | 0.0047 | 0.0096 | 0.0081 | 0.0055 | 0.0034 | 0.0042 | 0.0055 | 0.0043 |
Endoplasmic Reticulum | 0.6447 | 0.6532 | 0.688 | 0.7807 | 0.8255 | 0.8717 | 0.7999 | 0.7744 | 0.7886 | 0.7912 | 0.8118 | 0.8904 | 0.8565 | 0.8245 | 0.8487 | 0.8343 | 0.864 | 0.9067 |
Endosome | 0.0306 | 0.0027 | 0.0076 | 0.0083 | 0.0042 | 0.0037 | 0.0109 | 0.0053 | 0.0034 | 0.0023 | 0.0117 | 0.0049 | 0.0023 | 0.0021 | 0.0021 | 0.0043 | 0.008 | 0.003 |
Golgi | 0.0136 | 0.0086 | 0.01 | 0.0146 | 0.0037 | 0.0212 | 0.0108 | 0.0109 | 0.01 | 0.0173 | 0.0739 | 0.0117 | 0.0088 | 0.016 | 0.0064 | 0.0139 | 0.0463 | 0.0203 |
Lipid Particles | 0.0248 | 0.0388 | 0.0113 | 0.0053 | 0.0031 | 0.006 | 0.0146 | 0.0104 | 0.012 | 0.0022 | 0.0124 | 0.0027 | 0.0051 | 0.0178 | 0.0081 | 0.0095 | 0.0029 | 0.0049 |
Mitochondria | 0.0063 | 0.0025 | 0.0064 | 0.0023 | 0.0031 | 0.0006 | 0.0059 | 0.0012 | 0.0054 | 0.1 | 0.0018 | 0.0015 | 0.0007 | 0.0014 | 0.0005 | 0.0011 | 0.0005 | 0.0005 |
None | 0.0038 | 0.0003 | 0.0012 | 0.0035 | 0.0008 | 0.0002 | 0.0018 | 0.0004 | 0.0005 | 0.0006 | 0.0017 | 0.0003 | 0.0031 | 0.0002 | 0.0004 | 0.0002 | 0.0002 | 0.0002 |
Nuclear Periphery | 0.0358 | 0.0098 | 0.0135 | 0.0239 | 0.0477 | 0.0065 | 0.0218 | 0.0093 | 0.0095 | 0.0081 | 0.0069 | 0.007 | 0.0152 | 0.0084 | 0.0089 | 0.0091 | 0.0061 | 0.0045 |
Nucleolus | 0.0011 | 0.0001 | 0.0012 | 0.001 | 0.0006 | 0 | 0.0007 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0002 | 0.0001 | 0 | 0 |
Nucleus | 0.0167 | 0.0009 | 0.0055 | 0.01 | 0.0073 | 0.0004 | 0.0027 | 0.0005 | 0.0006 | 0.0005 | 0.0003 | 0.0006 | 0.0007 | 0.0003 | 0.0015 | 0.0006 | 0.0002 | 0.0003 |
Peroxisomes | 0.0077 | 0.0001 | 0.0013 | 0.0007 | 0 | 0 | 0.0101 | 0 | 0.0002 | 0.0004 | 0.0066 | 0.0007 | 0.0003 | 0 | 0 | 0.0001 | 0 | 0 |
Punctate Nuclear | 0.0075 | 0.0002 | 0.0027 | 0.0133 | 0.0014 | 0.0001 | 0.0037 | 0.0001 | 0.0002 | 0 | 0.0051 | 0.0005 | 0.0066 | 0.0001 | 0.0006 | 0.0001 | 0 | 0.0001 |
Vacuole | 0.0168 | 0.0101 | 0.0112 | 0.0103 | 0.003 | 0.003 | 0.0051 | 0.0088 | 0.0063 | 0.0051 | 0.0015 | 0.0047 | 0.0048 | 0.0052 | 0.0052 | 0.0074 | 0.0024 | 0.0029 |
Vacuole Periphery | 0.0042 | 0.0006 | 0.0018 | 0.0024 | 0.0031 | 0.0006 | 0.0018 | 0.0007 | 0.0011 | 0.0014 | 0.001 | 0.001 | 0.0009 | 0.0006 | 0.0006 | 0.0011 | 0.0006 | 0.0005 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 190.2421 | 168.1752 | 204.4366 | 261.5011 | 180.0598 | 158.4474 | 163.0682 | 199.8747 | 202.8771 | 226.9856 |
Translational Efficiency | 0.6571 | 0.5636 | 0.566 | 0.4011 | 0.4355 | 0.8391 | 0.598 | 0.4057 | 0.4692 | 0.424 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✔ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
225 | 1333 | 146 | 1563 | 2261 | 2484 | 988 | 1638 | 2486 | 3817 | 1134 | 3201 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 1270.45 | 1658.16 | 2393.54 | 1608.63 | 1618.04 | 1765.76 | 2169.28 | 1408.48 | 1586.58 | 1728.18 | 2198.15 | 1506.21 |
Standard Deviation | 276.71 | 318.26 | 326.08 | 323.29 | 373.39 | 306.68 | 324.34 | 299.35 | 379.04 | 314.98 | 333.14 | 326.95 |
Intensity Change Log 2 | — | 0.384244 | 0.913806 | 0.340493 | — | 0.126042 | 0.422969 | -0.200108 | — | 0.245333 | 0.659610 | 0.062852 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.024111 | 0.001629 | 0.001784 | 0.015677 | 0.001009 | 0.001679 | 0.002244 | 0.020460 | 0.003100 | 0.001662 | 0.002185 | 0.018125 |
Bud Neck | 0.008983 | 0.000974 | 0.005510 | 0.008303 | 0.000638 | 0.000598 | 0.002517 | 0.010765 | 0.001393 | 0.000730 | 0.002903 | 0.009563 |
Bud Site | 0.080371 | 0.007187 | 0.004595 | 0.066039 | 0.002340 | 0.004424 | 0.003215 | 0.126462* | 0.009403 | 0.005389 | 0.003393 | 0.096958 |
Cell Periphery | 0.053908 | 0.032390 | 0.244826* | 0.170724* | 0.046384 | 0.034194 | 0.176466* | 0.021939 | 0.047065 | 0.033564 | 0.185267* | 0.094589 |
Cytoplasm | 0.080267 | 0.001880 | 0.000088 | 0.029197 | 0.001804 | 0.001351 | 0.000125 | 0.093617 | 0.008906 | 0.001536 | 0.000120 | 0.062161 |
Cytoplasmic Foci | 0.035138 | 0.005623 | 0.000126 | 0.011721 | 0.002032 | 0.004192 | 0.000206 | 0.098397 | 0.005028 | 0.004691 | 0.000196 | 0.056074 |
Eisosomes | 0.003183 | 0.000827 | 0.008616 | 0.003897 | 0.003361 | 0.001711 | 0.010504 | 0.001622 | 0.003345 | 0.001402 | 0.010261 | 0.002733 |
Endoplasmic Reticulum | 0.437974* | 0.866996* | 0.610453* | 0.431192* | 0.900479* | 0.902206* | 0.722038* | 0.212869* | 0.858619* | 0.889909* | 0.707672* | 0.319473* |
Endosome | 0.031393 | 0.013462 | 0.007482 | 0.016709 | 0.007029 | 0.008989 | 0.006219 | 0.062718 | 0.009234 | 0.010551 | 0.006382 | 0.040253 |
Golgi | 0.024105 | 0.017792 | 0.038433 | 0.018350 | 0.007125 | 0.009117 | 0.012534 | 0.068097 | 0.008662 | 0.012147 | 0.015869 | 0.043806 |
Lipid Particles | 0.059437 | 0.038444 | 0.018496 | 0.184364* | 0.013590 | 0.018225 | 0.023399 | 0.207115* | 0.017739 | 0.025286 | 0.022768 | 0.196006* |
Mitochondria | 0.000897 | 0.001083 | 0.000267 | 0.003572 | 0.001138 | 0.001587 | 0.000675 | 0.007106 | 0.001116 | 0.001411 | 0.000623 | 0.005380 |
Mitotic Spindle | 0.002954 | 0.000268 | 0.000670 | 0.001461 | 0.000307 | 0.000225 | 0.001171 | 0.008186 | 0.000546 | 0.000240 | 0.001106 | 0.004903 |
None | 0.000069 | 0.000285 | 0.000046 | 0.000483 | 0.001326 | 0.000333 | 0.000105 | 0.002444 | 0.001212 | 0.000316 | 0.000097 | 0.001487 |
Nuclear Periphery | 0.000483 | 0.000739 | 0.008011 | 0.002475 | 0.002216 | 0.001494 | 0.014350 | 0.000279 | 0.002059 | 0.001231 | 0.013534 | 0.001351 |
Nuclear Periphery Foci | 0.011051 | 0.001531 | 0.002061 | 0.002494 | 0.000347 | 0.000365 | 0.000919 | 0.006511 | 0.001316 | 0.000772 | 0.001066 | 0.004550 |
Nucleolus | 0.000029 | 0.000014 | 0.000020 | 0.000048 | 0.000018 | 0.000020 | 0.000030 | 0.000388 | 0.000019 | 0.000018 | 0.000028 | 0.000222 |
Nucleus | 0.003787 | 0.000116 | 0.000161 | 0.001144 | 0.000427 | 0.000149 | 0.000191 | 0.002561 | 0.000731 | 0.000137 | 0.000187 | 0.001869 |
Peroxisomes | 0.002641 | 0.001983 | 0.000030 | 0.013880 | 0.006272 | 0.005872 | 0.003110 | 0.009882 | 0.005943 | 0.004514 | 0.002713 | 0.011835 |
Vacuole | 0.135481 | 0.004155 | 0.039967 | 0.014710 | 0.001344 | 0.001769 | 0.017572 | 0.029940 | 0.013484 | 0.002602 | 0.020456 | 0.022504 |
Vacuole Periphery | 0.003736 | 0.002624 | 0.008358 | 0.003560 | 0.000814 | 0.001502 | 0.002409 | 0.008641 | 0.001078 | 0.001894 | 0.003175 | 0.006160 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | 4.45 | 4.41 | 1.75 | -11.61 | -10.84 | -1.90 | -1.85 | -14.14 | -13.42 | -12.05 | 2.68 | 1.30 | -14.25 | -17.65 | -14.90 |
Bud Neck | 6.55 | 1.90 | 0.52 | -12.42 | -1.86 | 0.32 | -5.21 | -11.22 | -11.27 | -8.53 | 4.00 | -3.92 | -14.70 | -16.28 | -10.33 |
Bud Site | 6.37 | 6.57 | 1.34 | -14.94 | -15.33 | -2.55 | -1.12 | -21.92 | -21.43 | -21.64* | 3.11 | 4.48 | -23.73 | -26.02 | -26.53 |
Cell Periphery | 2.44 | -9.11 | -12.43 | -22.72* | 3.64 | 4.69 | -18.90 | 9.02 | 5.28 | 22.77* | 5.61 | -20.95 | -12.49 | -17.61 | 12.92 |
Cytoplasm | 8.18 | 8.37 | 5.29 | -12.90 | -13.89 | 1.04 | 4.12 | -22.82 | -23.04 | -23.37 | 7.01 | 8.41 | -20.47 | -25.66 | -26.32 |
Cytoplasmic Foci | 6.65 | 7.95 | 5.26 | -5.24 | -12.48 | -3.80 | 5.65 | -28.12 | -27.35 | -28.78* | 0.60 | 9.11 | -25.09 | -25.71 | -28.52 |
Eisosomes | 3.95 | -2.79 | -1.27 | -8.48 | 2.44 | 3.02 | -5.59 | 3.62 | 0.33 | 7.42 | 4.14 | -6.04 | 1.34 | -4.94 | 6.98 |
Endoplasmic Reticulum | -17.01 | -5.33 | -0.50 | 44.37* | 6.72 | -0.37 | 19.63 | 89.77* | 90.99* | 46.92* | -6.00 | 15.82 | 72.45 | 90.01* | 39.27* |
Endosome | 4.05 | 5.48 | 3.27 | -3.07 | -9.58 | -3.96 | 2.03 | -25.41 | -24.45 | -25.95* | -2.26 | 5.14 | -23.13 | -23.01 | -26.87 |
Golgi | 0.81 | -2.15 | 0.71 | -0.27 | 4.73 | -3.16 | -7.80 | -21.64 | -20.83 | -19.52* | -4.09 | -7.29 | -19.42 | -17.74 | -15.11 |
Lipid Particles | 2.81 | 5.12 | -13.57 | -22.05 | -22.88 | -3.15 | -4.39 | -30.11* | -29.39* | -27.61* | -4.71 | -2.39 | -39.03 | -37.47* | -36.29* |
Mitochondria | -0.53 | 2.11 | -5.27 | -5.54 | -7.84 | -1.34 | 2.43 | -6.18 | -5.45 | -6.62 | -1.23 | 2.89 | -7.86 | -6.91 | -8.70 |
Mitotic Spindle | 1.77 | 1.53 | 1.03 | -7.47 | -4.61 | 3.42 | -13.61 | -6.59 | -6.66 | -5.89 | 2.17 | -3.40 | -6.82 | -7.50 | -6.14 |
None | -4.04 | 1.21 | -2.33 | -1.11 | -2.53 | 2.07 | 2.54 | -1.32 | -3.17 | -3.52 | 2.06 | 2.55 | -0.44 | -3.33 | -3.97 |
Nuclear Periphery | -3.35 | -10.02 | -10.57 | -9.26 | 7.20 | 5.44 | -18.85 | 16.95 | 14.76 | 22.14 | 7.34 | -20.01 | 5.24 | -1.15 | 21.30 |
Nuclear Periphery Foci | 4.88 | 4.64 | 4.33 | -3.14 | -1.60 | -0.53 | -12.80 | -9.79 | -9.77 | -8.94 | 3.00 | 1.67 | -7.87 | -10.58 | -9.86 |
Nucleolus | 3.89 | 2.04 | -3.13 | -7.60 | -5.15 | -0.19 | -2.80 | -1.34 | -1.33 | -1.31 | 0.37 | -2.41 | -1.39 | -1.40 | -1.36 |
Nucleus | 5.39 | 5.33 | 3.94 | -5.80 | -5.52 | 1.60 | 1.40 | -4.24 | -5.15 | -5.07 | 3.53 | 3.28 | -3.51 | -6.72 | -6.55 |
Peroxisomes | 1.14 | 6.55 | -8.79 | -9.35 | -11.30 | 0.47 | 3.25 | -2.97 | -3.27 | -5.17 | 2.11 | 3.74 | -6.07 | -8.07 | -8.64 |
Vacuole | 9.68 | 7.00 | 8.94 | -9.97 | 5.29 | -2.11 | -17.54 | -15.91 | -15.62 | -6.45 | 7.54 | -2.95 | -4.30 | -18.70 | -1.83 |
Vacuole Periphery | 0.89 | -1.44 | 0.00 | -1.96 | 1.50 | -3.10 | -9.76 | -8.13 | -7.24 | -6.40 | -4.26 | -4.75 | -9.32 | -7.63 | -4.32 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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