Standard name
Human Ortholog
Description Protein with a role in translation; forms a complex with Gir2p; has similarity to mammalian developmentally regulated GTP-binding protein
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0.05 0.05 0 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0.07 0.05 0.07 0.07 0.08 0.05 0.07 0.08 0.11 0.05 0 0 0.05 0.08 0 0 0 0 0 0 0
Cytoplasm 0.96 0.98 0.98 0.94 0.94 0.92 0.81 0.72 0.74 0.72 0.66 0.74 0.98 0.98 0.98 0.93 0.94 0.97 0.9 0.97 0.91 0.91 0.91 0.93
Endoplasmic Reticulum 0 0 0 0 0 0 0.09 0.13 0 0.05 0 0.05 0 0 0 0 0 0.05 0 0 0 0.05 0.07 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0.12 0.13 0.22 0.18 0.33 0.16 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0.05 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 4 7 19 15 11 4 19 0 0 0 0 0 0 1 0 0 0 0 0
Bud 0 0 0 0 0 0 1 1 4 7 9 2 0 0 0 0 0 0 2 0 1 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 3 1 6 12 13 17 21 28 17 24 20 33 10 7 11 10 7 5 0 0 0 0 0 0
Cytoplasm 264 86 139 162 265 212 260 252 232 236 172 218 210 259 292 188 78 139 249 104 127 191 240 234
Endoplasmic Reticulum 5 1 3 5 12 7 28 44 14 17 9 15 2 6 3 9 3 7 8 1 5 9 17 9
Endosome 0 0 0 0 0 0 0 1 0 2 0 1 0 1 0 0 0 0 0 0 0 0 1 0
Golgi 0 0 0 0 0 0 0 2 0 5 0 0 0 0 0 0 1 0 1 0 0 0 0 1
Mitochondria 4 1 0 0 3 8 38 46 68 61 86 47 0 0 0 0 2 1 3 1 0 0 1 0
Nucleus 1 0 0 0 2 1 2 3 2 3 4 2 2 0 1 1 1 0 0 0 0 0 0 0
Nuclear Periphery 1 0 0 0 1 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 1 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 1 0 1 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0
Vac/Vac Membrane 1 1 1 1 9 1 6 9 9 15 14 9 0 1 0 8 0 2 2 0 0 1 0 1
Unique Cell Count 275 88 142 172 283 231 322 351 313 330 262 294 214 264 298 202 83 144 277 108 141 209 264 251
Labelled Cell Count 279 90 149 180 305 250 363 405 361 382 318 346 225 274 308 216 92 154 277 108 141 209 264 251


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 14.6 21.2 16.9 15.5 13.8 11.5 10.1 10.3 8.6 8.9 8.3 8.7 17.9 18.3 17.5 26.0 26.5 26.5 17.4 17.5 17.6
Std Deviation (1e-4) 3.0 4.0 3.1 2.7 2.2 2.2 1.9 2.1 1.5 1.8 1.8 1.5 3.5 3.1 3.3 7.2 7.2 7.0 3.1 3.1 3.1
Intensity Change (Log2) – – – -0.13 -0.3 -0.55 -0.74 -0.72 -0.97 -0.92 -1.02 -0.95 0.09 0.12 0.05 0.62 0.65 0.65 0.05 0.05 0.06


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 2.8 0 0 0 3.1 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 1.0 0.2 1.2 1.0 1.5 0.5 1.2 1.3 2.4 0.2 0 -0.3 0.3 0 -0.3
Cytoplasm -1.6 -1.9 -2.4 -4.9 -6.5 -6.1 -6.5 -7.3 -6.0 0.2 0.2 0.1 -2.0 -1.5 -0.7
Endoplasmic Reticulum 0 1.1 0 2.6 3.6 1.2 1.5 0 1.5 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 4.3 4.5 6.0 5.5 7.7 5.0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 2.4 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration – – – – – – – – – – – – – – – – – –
Actin – – – – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – – – – –
Bud Periphery – – – – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – – – – –
Cytoplasmic Foci – – – – – – – – – – – – – – – – – –
Eisosomes – – – – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – – – – –
Lipid Particles – – – – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – – – – –
None – – – – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – – – – –
Punctate Nuclear – – – – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – – – – –
Vacuole Periphery – – – – – – – – – – – – – – – – – –

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 72.7629 59.6994 60.8292 50.7297 69.1398 54.791 52.2536 39.2201 46.6584 55.165
Translational Efficiency 1.7002 1.8488 1.3871 1.7361 1.3494 1.7883 1.7304 1.9478 1.5115 1.71

Hit Data

Dataset Hit
Protein Concentration –
Protein Localization –
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
86 1597 986 978 1825 1354 1269 633 1911 2951 2255 1611

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 1314.16 1583.53 1714.74 2238.44 1401.16 1558.15 1892.98 2293.09 1397.24 1571.88 1815.04 2259.91
Standard Deviation 1044.91 261.98 275.40 427.20 209.56 284.81 286.53 454.46 302.33 272.99 295.26 438.92
Intensity Change Log 2 — 0.269003 0.383849 0.768353 — 0.153212 0.434037 0.710671 — 0.210414 0.409965 0.738876

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.001182 0.000150 0.000445 0.000630 0.000131 0.000292 0.000272 0.001966 0.000178 0.000215 0.000348 0.001155
Bud Neck 0.000924 0.000150 0.001300 0.003830 0.000169 0.000210 0.001497 0.003832 0.000203 0.000178 0.001411 0.003831
Bud Site 0.075102 0.007751 0.013930 0.021735 0.008153 0.010676 0.009527 0.031193 0.011166 0.009093 0.011452 0.025451
Cell Periphery 0.003164 0.000348 0.002474 0.007664 0.000245 0.000384 0.000448 0.006929 0.000376 0.000365 0.001334 0.007375
Cytoplasm 0.504014* 0.931700* 0.905002* 0.641941* 0.877044* 0.922338* 0.860678* 0.669138* 0.860257* 0.927404* 0.880059* 0.652627*
Cytoplasmic Foci 0.216864* 0.030422 0.016310 0.017813 0.067999 0.029387 0.018856 0.020645 0.074698 0.029947 0.017743 0.018926
Eisosomes 0.000021 0.000001 0.000003 0.000007 0.000002 0.000002 0.000002 0.000008 0.000003 0.000002 0.000003 0.000007
Endoplasmic Reticulum 0.015369 0.001299 0.000883 0.001484 0.004916 0.001220 0.000994 0.000593 0.005387 0.001263 0.000946 0.001134
Endosome 0.016648 0.001461 0.002101 0.009879 0.002551 0.001830 0.003837 0.007651 0.003185 0.001630 0.003078 0.009004
Golgi 0.033376 0.010409 0.008794 0.023032 0.010377 0.013354 0.007053 0.017196 0.011412 0.011760 0.007815 0.020739
Lipid Particles 0.006075 0.001182 0.007545 0.020804 0.000835 0.001069 0.003745 0.023897 0.001071 0.001130 0.005407 0.022019
Mitochondria 0.002476 0.000400 0.000035 0.001546 0.000172 0.000284 0.000066 0.000473 0.000275 0.000347 0.000052 0.001124
Mitotic Spindle 0.005716 0.000642 0.002253 0.006646 0.000862 0.002002 0.002521 0.008676 0.001080 0.001266 0.002404 0.007444
None 0.000992 0.001501 0.000581 0.000318 0.000815 0.003156 0.000347 0.000197 0.000823 0.002260 0.000449 0.000271
Nuclear Periphery 0.000344 0.000011 0.000047 0.000258 0.000017 0.000010 0.000073 0.000261 0.000031 0.000011 0.000062 0.000259
Nuclear Periphery Foci 0.011880 0.001013 0.004314 0.005599 0.000644 0.001195 0.003233 0.005750 0.001150 0.001097 0.003706 0.005658
Nucleolus 0.002696 0.000185 0.000029 0.000263 0.000076 0.000028 0.000072 0.000197 0.000194 0.000113 0.000053 0.000237
Nucleus 0.014837 0.002181 0.006907 0.007310 0.002457 0.003521 0.009192 0.004372 0.003014 0.002796 0.008193 0.006156
Peroxisomes 0.000939 0.000079 0.000139 0.000537 0.000117 0.000139 0.000257 0.000698 0.000154 0.000107 0.000205 0.000600
Vacuole 0.048289 0.004517 0.024694 0.210104* 0.010319 0.004828 0.072428 0.183437 0.012027 0.004660 0.051557 0.199626
Vacuole Periphery 0.039094 0.004597 0.002215 0.018600 0.012100 0.004075 0.004901 0.012894 0.013315 0.004357 0.003726 0.016358

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 2.39 1.64 1.33 -4.58 -1.16 -1.81 -5.28 -1.57 -1.41 -1.41 -0.73 -5.26 -2.13 -2.03 -1.64
Bud Neck 4.76 -2.08 -10.85 -15.00 -10.04 -1.94 -10.34 -12.68 -12.55 -7.50 1.43 -13.33 -19.40 -19.53 -12.18
Bud Site 6.18 5.54 4.87 -9.30 -4.02 -2.40 -2.27 -8.60 -7.34 -7.58 2.19 -0.98 -9.31 -11.32 -8.88
Cell Periphery 2.04 0.47 -2.07 -8.43 -4.62 -3.26 -5.67 -7.98 -7.78 -7.69 0.14 -4.62 -11.23 -11.32 -8.85
Cytoplasm -12.75 -11.79 -3.73 28.17 24.13 -7.16 3.84 17.82 21.14 15.05 -12.19 -1.98 24.50 35.07 27.00
Cytoplasmic Foci 7.74 8.32 8.25 5.28 -0.78 11.14 15.97 14.15 3.13 -0.67 13.70 18.29 17.36 6.07 -0.68
Eisosomes 3.59 3.30 2.68 -7.71 -5.03 0.09 -2.60 -5.13 -5.08 -4.47 3.37 0.03 -6.13 -8.81 -6.81
Endoplasmic Reticulum 2.76 2.84 2.77 0.19 -0.58 4.43 4.99 5.59 2.12 2.38 5.16 5.66 5.36 0.94 0.03
Endosome 5.43 5.18 2.73 -6.17 -5.51 2.34 -3.43 -3.78 -4.34 -2.55 5.96 0.16 -5.54 -7.50 -5.59
Golgi 4.53 4.85 2.67 -4.29 -5.09 -2.04 5.11 -2.33 -0.62 -4.12 -0.41 5.60 -4.20 -3.62 -6.82
Lipid Particles 2.29 -0.71 -5.48 -11.92 -7.27 -1.50 -9.39 -10.71 -10.59 -9.20 -0.39 -11.40 -15.98 -15.95 -12.13
Mitochondria 1.58 1.89 0.91 -1.30 -2.11 -1.01 3.71 -1.17 -0.47 -1.79 -0.41 3.48 -1.73 -1.41 -2.41
Mitotic Spindle 1.60 1.10 -0.13 -4.05 -2.74 -1.94 -2.25 -2.58 -2.17 -1.90 -0.37 -2.32 -4.15 -4.16 -3.16
None -0.59 0.74 1.17 1.92 2.39 -3.83 2.68 3.61 5.05 3.76 -3.14 2.25 3.19 4.64 2.78
Nuclear Periphery 1.60 1.43 0.53 -5.54 -4.64 2.18 -7.93 -2.51 -2.58 -1.79 2.08 -3.02 -4.71 -5.34 -4.03
Nuclear Periphery Foci 1.37 0.97 0.83 -10.11 -2.06 -4.31 -9.38 -5.45 -4.83 -2.37 0.24 -5.76 -7.47 -9.91 -3.55
Nucleolus 1.57 1.67 1.54 -0.39 -5.79 0.96 -0.04 -2.25 -10.70 -7.60 0.71 1.61 -0.23 -1.21 -7.08
Nucleus 1.48 0.93 0.57 -8.92 -3.18 -2.65 -13.13 -6.12 -4.86 6.92 0.68 -9.67 -7.19 -9.84 0.86
Peroxisomes 3.94 3.63 1.97 -4.25 -3.43 -0.67 -1.26 -5.21 -4.79 -2.77 2.25 -0.76 -5.51 -6.17 -3.77
Vacuole 3.65 1.83 -12.40 -28.81 -25.05 5.71 -16.59 -21.26 -21.85 -12.67 7.66 -16.30 -34.62 -36.14 -25.22
Vacuole Periphery 2.95 3.16 2.02 -6.39 -8.86 6.29 6.41 0.70 -4.17 -4.03 7.07 8.28 -0.16 -7.72 -9.11
Protein Complexes26S Proteasome complex37S mitochondrial small ribosomal subunit54S mitochondrial large ribosomal subunit60S cytosolic large ribosomal subunitAar2-U5 snRNPAnaphase-Promoting Complex variant 1Anaphase-Promoting Complex variant 2Anaphase-Promoting Complex variant 3Anaphase-Promoting Complex variant 4Arp2/3 complexATG1 kinase complexBLOC-1 complexCcr4-Not complexCentral kinetochore CTF19 complexCleavage and polyadenylation specificity factorcomplexCMG helicase complexCMG-Pol epsilon complexCOMPASS complexCOP9 signalosome COPI vesicle coat complexCoQ6 biosynthesis complexCore mediator complexCORVET complexCTF18-RFC complexCURI complex variant 1CURI complex variant 2CURI complex variant 3Cytoplasmic exosome complex, DIS3 variantDASH complexDNA-directed RNA Polymerase I complexDNA-directed RNA polymerase II complexDNA-directed RNA polymerase III complexDNA replication factor C complexEGO complexELG1-RFC complex Elongator holoenzyme complexEndoplasmic Reticulum Membrane ComplexEukaryotic translation initiation factor 2BcomplexEukaryotic translation initiation factor 3core complexExocystExomer complexFAR complexGeneral transcription factor complex TFIIDGeneral transcription factor complex TFIIHGET4-GET5 transmembrane domain recognition complexGID ubiquitin ligase complexGin4 complexGolgi transport complexGSE complexHMC complexHOPS complexINO80 chromatin remodeling complexLSM1-7 complexLsm1-7-Pat1 complexLSM2-7 complexLSM2-8 complexMCM complexMICOS complexMitochondrial electron transport complex IIIMitochondrial inner membrane pre-sequence translocasecomplexMitochondrial outer membrane translocase holocomplexMitochondrial proton-transporting ATP synthase complexMitochondrial pyruvate dehydrogenase complexNineTeen complexNOP8 60s ribosome pre-assembly complexNuA3 histone acetyltransferase complexNuA4 histone acetyltransferase complexNuclear condensin complexNuclear/nucleolar exosome complex, DIS3-RRP6 variantNuclear pore complexNucleolar ribonuclease MRP complexNucleolar ribonuclease P complexOligosaccharyl transferase complex variant 1Oligosaccharyl transferase complex variant 2PAF1 complexPAS complexPhosphatidylinositol 3-kinase complex IPolarisomePrefoldin complexPRP19-associated complexRad17 RFC-like complexRetromer complexRPD3L histone deacetylase complexRPD3S histone deacetylase complexRQC complexRSC complex variant RSC1RSC complex variant RSC2SAGA complexSCF-Met30 ubiquitin ligase complexSCF-Ufo1 ubiquitin ligase complexSEA complexSeptin complexSET3C histone deacetylase complexSignal recognition particleSLIK (SAGA-like) complexSmall ribosomal subunit processome, variant1Small ribosomal subunit processome, variant2Small ribosomal subunit processome, variant3SMC5-SMC6 SUMO ligase complexSm complexSpliceosomal commitment complexSPOTS complexSWI/SNF chromatin remodelling complexSwr1 chromatin remodelling complexTHO complexTranscription factor TFIIIC complexTranslocon complexTRAPPIII protein complexTRAPPII protein complexTRAPPI protein complexTREX complexU1 snRNPU2 snRNPU4 snRNPU4/U6 snRNPU4/U6.U5 tri-snRNP complexU5 snRNPU6 snRNP complexUTP-A complexUTP-B complexUTP-C complex variant 1UTP-C complex variant 2UTP-C complex variant 3Vacuolar proton translocating ATPase complex,Golgi variantVacuolar proton translocating ATPase complex,vacuole variantCellular Components••••••••••••••••actinbud neckcell peripherycytoplasmendosomeERGolgimitochondrionmixednuclear peripherynucleolusnucleusperoxisomespindle polevacuolar membranevacuole
Standard name
Human Ortholog
Description Protein with a role in translation; forms a complex with Gir2p; has similarity to mammalian developmentally regulated GTP-binding protein
Localization
Cell Percentages cytoplasm (99%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-7

Rbg2

Rbg2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Rbg2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available