Standard name | |
---|---|
Human Ortholog | |
Description | Ribonucleotide-diphosphate reductase (RNR) small subunit; the RNR complex catalyzes the rate-limiting step in dNTP synthesis and is regulated by DNA replication and DNA damage checkpoint pathways via localization of the small subunits; relocalizes from nucleus to cytoplasm upon DNA replication stress; RNR4 has a paralog, RNR2, that arose from the whole genome duplication |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 |
Bud | 0 | 0 | 0 | 1 | 2 | 0 | 1 | 1 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 5 | 3 | 1 | 0 | 1 | 4 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 1 | 0 | 0 | 2 | 7 | 13 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 54 | 20 | 14 | 22 | 45 | 43 | 61 | 56 | 30 | 41 | 36 | 36 | 106 | 150 | 126 | 56 | 18 | 41 | 32 | 10 | 10 | 8 | 4 | 2 |
Endoplasmic Reticulum | 0 | 1 | 0 | 0 | 8 | 1 | 7 | 10 | 3 | 2 | 0 | 3 | 0 | 1 | 0 | 26 | 8 | 8 | 12 | 2 | 3 | 2 | 7 | 5 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 |
Golgi | 1 | 0 | 1 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 2 | 0 |
Mitochondria | 5 | 1 | 1 | 1 | 2 | 11 | 9 | 12 | 3 | 16 | 16 | 21 | 0 | 0 | 0 | 12 | 1 | 4 | 0 | 0 | 0 | 0 | 3 | 4 |
Nucleus | 116 | 39 | 33 | 63 | 75 | 132 | 157 | 191 | 129 | 187 | 143 | 139 | 18 | 3 | 3 | 163 | 56 | 126 | 126 | 53 | 50 | 63 | 161 | 172 |
Nuclear Periphery | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 12 | 3 | 3 | 6 | 18 | 19 |
Nucleolus | 22 | 6 | 5 | 9 | 16 | 18 | 38 | 37 | 14 | 31 | 30 | 36 | 5 | 0 | 1 | 18 | 6 | 12 | 13 | 2 | 4 | 8 | 35 | 30 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 1 | 0 | 3 | 1 | 7 | 1 | 2 | 3 | 1 | 0 | 0 | 4 | 1 | 2 | 1 | 24 | 7 | 10 | 8 | 2 | 2 | 8 | 8 | 10 |
Unique Cell Count | 184 | 61 | 51 | 87 | 148 | 183 | 244 | 273 | 155 | 239 | 182 | 186 | 127 | 158 | 141 | 268 | 89 | 187 | 226 | 83 | 82 | 105 | 267 | 268 |
Labelled Cell Count | 201 | 67 | 57 | 97 | 156 | 207 | 275 | 311 | 181 | 278 | 227 | 240 | 133 | 163 | 148 | 299 | 97 | 203 | 226 | 83 | 82 | 105 | 267 | 268 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 172.0 | 125.0 | 172.4 | 153.1 | 155.2 | 168.2 | 169.3 | 171.3 | 175.2 | 171.8 | 161.8 | 167.9 | 248.2 | 324.4 | 340.4 | 215.9 | 221.3 | 224.5 | 184.0 | 174.1 | 168.2 |
Std Deviation (1e-4) | 47.5 | 58.7 | 52.8 | 47.2 | 43.9 | 47.7 | 52.2 | 53.4 | 49.1 | 50.2 | 54.1 | 49.6 | 75.9 | 135.3 | 157.5 | 62.2 | 71.8 | 60.5 | 47.7 | 52.4 | 60.0 |
Intensity Change (Log2) | – | – | – | -0.17 | -0.15 | -0.04 | -0.03 | -0.01 | 0.02 | -0.0 | -0.09 | -0.04 | 0.53 | 0.91 | 0.98 | 0.32 | 0.36 | 0.38 | 0.09 | 0.01 | -0.04 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | -0.3 | 0.4 | -0.6 | -0.4 | -1.1 | -1.2 | -1.7 | -1.2 | -1.3 | 7.2 | 10.2 | 8.5 | -1.0 | -1.0 | -0.8 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0.9 | -1.7 | 1.0 | 0 | 0.7 | 2.8 | 2.1 | 2.0 | 1.4 | -6.7 | -10.3 | -9.8 | -0.5 | -0.2 | 0.4 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0.1 | 0.2 | 0 | 1.1 | 0.7 | 0 | 0.6 | 1.2 | 1.6 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 108.5885 | 134.5827 | 125.9553 | 128.7064 | 123.6713 | 140.0585 | 64.4327 | 120.0859 | 113.3171 | 120.4086 | 92.2192 | 130.951 | 111.8568 | 133.6405 | 127.8679 | 132.6923 | 102.3751 | 133.5785 |
Actin | 0.001 | 0.0004 | 0.0034 | 0.0005 | 0.0031 | 0.0001 | 0.0051 | 0.0003 | 0.0022 | 0.0054 | 0.0082 | 0.0002 | 0.0279 | 0.0004 | 0.0108 | 0.0112 | 0.027 | 0.0001 |
Bud | 0.0008 | 0.0023 | 0.005 | 0.0013 | 0.0006 | 0.0007 | 0.0034 | 0.0014 | 0.0039 | 0.0014 | 0.0003 | 0.0002 | 0.0021 | 0.0013 | 0.0027 | 0.0018 | 0.0027 | 0.0001 |
Bud Neck | 0.0002 | 0.0002 | 0.0005 | 0.0005 | 0.0012 | 0.0005 | 0.0003 | 0.0002 | 0.0005 | 0.0004 | 0.0002 | 0.0004 | 0.0011 | 0.0001 | 0.0004 | 0.0007 | 0.0022 | 0.0004 |
Bud Periphery | 0.0004 | 0.0004 | 0.0013 | 0.0005 | 0.0003 | 0.0003 | 0.0025 | 0.0004 | 0.0011 | 0.0014 | 0.0005 | 0.0001 | 0.0017 | 0.0003 | 0.0006 | 0.0004 | 0.0028 | 0 |
Bud Site | 0.0004 | 0.0023 | 0.0028 | 0.0003 | 0.0005 | 0.0001 | 0.0022 | 0.0008 | 0.0025 | 0.0006 | 0.0001 | 0.0001 | 0.0079 | 0.0005 | 0.0007 | 0.0019 | 0.0108 | 0 |
Cell Periphery | 0.0004 | 0.0052 | 0.007 | 0.0029 | 0.0019 | 0.0006 | 0.0006 | 0.0019 | 0.0047 | 0.0019 | 0.0002 | 0.0027 | 0.0026 | 0.0011 | 0.0016 | 0.001 | 0.0009 | 0.0001 |
Cytoplasm | 0.0062 | 0.269 | 0.3998 | 0.5784 | 0.3075 | 0.0608 | 0.0419 | 0.255 | 0.548 | 0.7116 | 0.1722 | 0.1658 | 0.0135 | 0.3341 | 0.6273 | 0.7518 | 0.3032 | 0.0978 |
Cytoplasmic Foci | 0.0044 | 0.002 | 0.0086 | 0.0056 | 0.0098 | 0.0021 | 0.0162 | 0.0007 | 0.0048 | 0.0016 | 0.0006 | 0.0013 | 0.0312 | 0.0005 | 0.002 | 0.0055 | 0.0133 | 0.0001 |
Eisosomes | 0.0006 | 0.0003 | 0.0003 | 0.0002 | 0.0012 | 0 | 0.0002 | 0.0002 | 0.0002 | 0.0001 | 0.0001 | 0 | 0.0008 | 0 | 0.0002 | 0.0003 | 0.0004 | 0 |
Endoplasmic Reticulum | 0.0002 | 0.0005 | 0.0015 | 0.0009 | 0.001 | 0.0004 | 0.0047 | 0.0003 | 0.0028 | 0.0041 | 0.003 | 0.0018 | 0.0014 | 0.0004 | 0.0012 | 0.0004 | 0.0052 | 0 |
Endosome | 0.0001 | 0.0002 | 0.0037 | 0.0011 | 0.0006 | 0.0005 | 0.0242 | 0.0001 | 0.0049 | 0.0042 | 0.0132 | 0.0012 | 0.0072 | 0.0001 | 0.0018 | 0.0044 | 0.0272 | 0 |
Golgi | 0.0001 | 0 | 0.0009 | 0.0001 | 0.0002 | 0 | 0.0012 | 0 | 0.002 | 0.0016 | 0.0037 | 0 | 0.0021 | 0 | 0.0006 | 0.0007 | 0.0082 | 0 |
Lipid Particles | 0.001 | 0.001 | 0.0018 | 0.0013 | 0.0034 | 0.0002 | 0.003 | 0.0003 | 0.0008 | 0.0007 | 0.0012 | 0.0004 | 0.0117 | 0.0001 | 0.0013 | 0.0029 | 0.0279 | 0 |
Mitochondria | 0.02 | 0.0032 | 0.0103 | 0.0101 | 0.0349 | 0.0036 | 0.0419 | 0.0047 | 0.0153 | 0.0058 | 0.0206 | 0.0007 | 0.0149 | 0.0062 | 0.0079 | 0.0044 | 0.011 | 0.001 |
None | 0.0026 | 0.0029 | 0.0046 | 0.0059 | 0.0073 | 0.0002 | 0.006 | 0.0021 | 0.0043 | 0.0012 | 0.0007 | 0.0019 | 0.016 | 0.0008 | 0.003 | 0.0028 | 0.0083 | 0.0001 |
Nuclear Periphery | 0.0039 | 0.0147 | 0.0231 | 0.0233 | 0.0473 | 0.0109 | 0.0468 | 0.0127 | 0.0283 | 0.0352 | 0.1785 | 0.0131 | 0.0176 | 0.018 | 0.0375 | 0.0177 | 0.0607 | 0.0317 |
Nucleolus | 0.1657 | 0.064 | 0.0924 | 0.0504 | 0.0386 | 0.0584 | 0.061 | 0.0493 | 0.0445 | 0.0191 | 0.0602 | 0.0218 | 0.0816 | 0.0314 | 0.0318 | 0.0113 | 0.0245 | 0.0335 |
Nucleus | 0.7817 | 0.6141 | 0.4016 | 0.2933 | 0.5254 | 0.856 | 0.6872 | 0.6594 | 0.2967 | 0.1898 | 0.514 | 0.7298 | 0.7358 | 0.5955 | 0.2545 | 0.171 | 0.3831 | 0.8328 |
Peroxisomes | 0.0004 | 0.0001 | 0.0005 | 0.0002 | 0.0005 | 0.0001 | 0.0008 | 0.0001 | 0.0003 | 0.0001 | 0.0001 | 0 | 0.0015 | 0.0001 | 0.002 | 0.0016 | 0.0204 | 0 |
Punctate Nuclear | 0.0064 | 0.0015 | 0.0024 | 0.0019 | 0.0028 | 0.0011 | 0.0304 | 0.0014 | 0.0014 | 0.0021 | 0.0023 | 0.0018 | 0.0062 | 0.0007 | 0.0018 | 0.0018 | 0.0409 | 0.0006 |
Vacuole | 0.0018 | 0.0121 | 0.0193 | 0.0144 | 0.0077 | 0.0016 | 0.0089 | 0.0053 | 0.0129 | 0.0064 | 0.003 | 0.0476 | 0.0104 | 0.0041 | 0.0055 | 0.0034 | 0.0129 | 0.0003 |
Vacuole Periphery | 0.0018 | 0.0036 | 0.0092 | 0.0069 | 0.0042 | 0.0016 | 0.0114 | 0.0034 | 0.0178 | 0.0053 | 0.017 | 0.009 | 0.0046 | 0.0042 | 0.0048 | 0.003 | 0.0063 | 0.0012 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 643.1211 | 654.7333 | 660.7147 | 616.5614 | 628.477 | 618.3294 | 593.5423 | 587.2023 | 566.3162 | 513.2685 |
Translational Efficiency | 1.6129 | 1.6629 | 1.4957 | 1.6796 | 1.5336 | 1.4722 | 1.5418 | 1.5436 | 1.6025 | 1.6299 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✔ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
2346 | 1014 | 2187 | 1150 | 1502 | 1531 | 429 | 295 | 3848 | 2545 | 2616 | 1445 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 11985.19 | 12947.21 | 9237.07 | 12357.38 | 11684.71 | 11481.49 | 11902.57 | 10165.37 | 11867.90 | 12065.47 | 9674.19 | 11909.88 |
Standard Deviation | 3137.96 | 3121.42 | 2165.97 | 3363.77 | 3316.04 | 3003.09 | 3368.60 | 3548.84 | 3211.99 | 3134.04 | 2599.42 | 3515.22 |
Intensity Change Log 2 | — | 0.111388 | -0.375746 | 0.044120 | — | -0.025312 | 0.026651 | -0.200959 | — | 0.045523 | -0.163103 | -0.071671 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.002757 | 0.004059 | 0.000515 | 0.000993 | 0.001793 | 0.002034 | 0.003011 | 0.001521 | 0.002381 | 0.002841 | 0.000924 | 0.001101 |
Bud Neck | 0.017069 | 0.022938 | 0.044867 | 0.022586 | 0.018556 | 0.023857 | 0.028009 | 0.023919 | 0.017650 | 0.023491 | 0.042103 | 0.022858 |
Bud Site | 0.001450 | 0.002108 | 0.009457 | 0.006193 | 0.001817 | 0.002692 | 0.003836 | 0.026270 | 0.001593 | 0.002460 | 0.008535 | 0.010292 |
Cell Periphery | 0.001518 | 0.002007 | 0.006204 | 0.020322 | 0.001354 | 0.000918 | 0.001186 | 0.006437 | 0.001454 | 0.001352 | 0.005381 | 0.017488 |
Cytoplasm | 0.014785 | 0.027037 | 0.398849* | 0.289073* | 0.026784 | 0.018120 | 0.034603 | 0.121345* | 0.019468 | 0.021673 | 0.339116* | 0.254831* |
Cytoplasmic Foci | 0.002102 | 0.007942 | 0.009453 | 0.015168 | 0.005097 | 0.005768 | 0.010464 | 0.025642 | 0.003271 | 0.006634 | 0.009618 | 0.017306 |
Eisosomes | 0.000575 | 0.000541 | 0.000012 | 0.000031 | 0.000420 | 0.000242 | 0.000129 | 0.000064 | 0.000514 | 0.000361 | 0.000031 | 0.000038 |
Endoplasmic Reticulum | 0.003099 | 0.001153 | 0.000560 | 0.000343 | 0.000800 | 0.000792 | 0.000637 | 0.003033 | 0.002202 | 0.000936 | 0.000572 | 0.000892 |
Endosome | 0.000083 | 0.000136 | 0.000998 | 0.001831 | 0.000084 | 0.000095 | 0.000345 | 0.021673 | 0.000083 | 0.000111 | 0.000891 | 0.005882 |
Golgi | 0.000088 | 0.000527 | 0.002308 | 0.003540 | 0.000338 | 0.000158 | 0.001502 | 0.007665 | 0.000185 | 0.000305 | 0.002176 | 0.004382 |
Lipid Particles | 0.000214 | 0.001252 | 0.008198 | 0.017199 | 0.000629 | 0.000240 | 0.002832 | 0.011391 | 0.000376 | 0.000643 | 0.007318 | 0.016014 |
Mitochondria | 0.003044 | 0.005733 | 0.003584 | 0.005728 | 0.003231 | 0.003495 | 0.010362 | 0.010865 | 0.003117 | 0.004386 | 0.004696 | 0.006777 |
Mitotic Spindle | 0.000118 | 0.000275 | 0.024756 | 0.027123 | 0.000192 | 0.001311 | 0.012417 | 0.071165 | 0.000147 | 0.000898 | 0.022733 | 0.036114 |
None | 0.001728 | 0.003885 | 0.005264 | 0.005465 | 0.001510 | 0.002705 | 0.002735 | 0.005217 | 0.001643 | 0.003175 | 0.004849 | 0.005415 |
Nuclear Periphery | 0.004378 | 0.007586 | 0.010546 | 0.003549 | 0.001350 | 0.003401 | 0.019521 | 0.002779 | 0.003196 | 0.005069 | 0.012018 | 0.003392 |
Nuclear Periphery Foci | 0.000283 | 0.001701 | 0.007774 | 0.006089 | 0.000705 | 0.000436 | 0.006437 | 0.006061 | 0.000448 | 0.000940 | 0.007554 | 0.006083 |
Nucleolus | 0.094366 | 0.231216* | 0.019651 | 0.038018 | 0.103811 | 0.127958 | 0.290578* | 0.086835 | 0.098053 | 0.169099 | 0.064080 | 0.047984 |
Nucleus | 0.827498* | 0.651857* | 0.400477* | 0.427137* | 0.817347* | 0.793736* | 0.522112* | 0.434161* | 0.823536* | 0.737208* | 0.420424* | 0.428571* |
Peroxisomes | 0.001188 | 0.005459 | 0.000393 | 0.001997 | 0.003511 | 0.003438 | 0.018241 | 0.010369 | 0.002095 | 0.004243 | 0.003320 | 0.003706 |
Vacuole | 0.013047 | 0.009942 | 0.041106 | 0.101375 | 0.005386 | 0.004115 | 0.020626 | 0.103291 | 0.010057 | 0.006436 | 0.037747 | 0.101766 |
Vacuole Periphery | 0.010612 | 0.012648 | 0.005029 | 0.006238 | 0.005285 | 0.004490 | 0.010419 | 0.020297 | 0.008532 | 0.007740 | 0.005913 | 0.009108 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -3.30 | 11.26 | 8.57 | 9.77 | -3.87 | -1.13 | -3.86 | 3.79 | 3.84 | 6.14 | -1.86 | 11.21 | 9.49 | 10.10 | -1.00 |
Bud Neck | -4.25 | -18.38 | -7.76 | -2.99 | 9.49 | -2.94 | -4.92 | -5.74 | -4.41 | -1.15 | -5.55 | -18.76 | -9.27 | -4.86 | 8.24 |
Bud Site | -4.13 | -15.75 | -9.20 | -7.27 | 4.28 | -3.35 | -4.01 | -4.74 | -4.36 | -3.48 | -5.32 | -16.04 | -9.07 | -7.41 | 0.61 |
Cell Periphery | -2.11 | -5.58 | -7.77 | -6.86 | -4.97 | 1.83 | -0.61 | -1.82 | -2.38 | -1.56 | -0.29 | -5.68 | -7.96 | -7.88 | -5.02 |
Cytoplasm | -4.16 | -39.45 | -22.38 | -19.97 | 7.79 | 2.02 | -2.95 | -6.37 | -7.01 | -4.13 | -1.94 | -37.17 | -22.61 | -21.87 | 7.04 |
Cytoplasmic Foci | -5.13 | -10.08 | -8.17 | -2.28 | -2.77 | -0.69 | -2.80 | -4.62 | -4.35 | -1.90 | -4.95 | -8.57 | -8.80 | -5.15 | -3.52 |
Eisosomes | 0.43 | 10.40 | 10.08 | 3.19 | -7.25 | 2.04 | 3.45 | 4.00 | 6.55 | 4.02 | 2.22 | 10.26 | 10.09 | 4.90 | -2.82 |
Endoplasmic Reticulum | 6.28 | 8.31 | 9.25 | 6.09 | 6.22 | 0.32 | 1.27 | -0.83 | -0.94 | -1.22 | 6.54 | 8.23 | 7.60 | 2.69 | 0.82 |
Endosome | -3.67 | -7.64 | -2.62 | -2.36 | 0.03 | -0.64 | -3.53 | -2.47 | -2.47 | -2.33 | -3.09 | -8.03 | -3.38 | -3.29 | -1.98 |
Golgi | -3.46 | -10.85 | -8.36 | -5.55 | -1.06 | 1.96 | -4.63 | -3.08 | -3.27 | -1.72 | -2.33 | -11.10 | -7.94 | -7.24 | -1.87 |
Lipid Particles | -2.33 | -8.27 | -8.44 | -6.93 | -3.02 | 1.02 | -2.19 | -3.78 | -3.92 | -2.54 | -1.46 | -8.41 | -9.14 | -8.69 | -3.75 |
Mitochondria | -8.45 | -5.81 | -5.84 | -0.22 | -2.72 | -1.11 | -4.61 | -2.85 | -2.62 | 1.60 | -6.59 | -7.43 | -6.47 | -3.54 | -1.37 |
Mitotic Spindle | -2.32 | -12.98 | -8.87 | -8.77 | -1.16 | -1.90 | -4.22 | -6.24 | -6.10 | -4.07 | -2.29 | -13.61 | -10.80 | -10.49 | -3.02 |
None | -2.46 | -10.57 | -8.36 | -1.82 | -0.40 | -3.09 | -2.97 | -4.62 | -2.98 | -2.76 | -3.75 | -11.54 | -9.78 | -4.08 | -1.48 |
Nuclear Periphery | -2.55 | -5.80 | -1.54 | 1.50 | 4.69 | -1.82 | -5.32 | -3.55 | -2.15 | 3.34 | -2.25 | -8.30 | -3.74 | -0.91 | 5.64 |
Nuclear Periphery Foci | -3.07 | -9.56 | -8.64 | -3.39 | 2.52 | -0.02 | -3.90 | -3.53 | -3.42 | 0.84 | -2.66 | -10.00 | -8.93 | -5.94 | 2.67 |
Nucleolus | -11.33 | 15.29 | 11.47 | 17.77 | -2.44 | -2.16 | -8.20 | 3.26 | 4.79 | 9.44 | -9.40 | 8.28 | 12.29 | 18.70 | 4.15 |
Nucleus | 14.78 | 42.60 | 25.42 | 9.52 | -5.07 | 2.16 | 15.45 | 13.46 | 12.55 | 1.05 | 11.58 | 45.87 | 28.75 | 19.58 | -4.26 |
Peroxisomes | -4.38 | 4.34 | -1.86 | 3.72 | -5.36 | 0.31 | -4.79 | -2.17 | -2.27 | 2.26 | -4.30 | -1.62 | -1.88 | 1.69 | -0.34 |
Vacuole | 1.12 | -10.27 | -11.68 | -11.98 | -6.00 | 0.58 | -4.54 | -7.40 | -7.45 | -4.40 | 2.47 | -12.23 | -14.15 | -14.74 | -7.37 |
Vacuole Periphery | -0.91 | 5.46 | 5.61 | 5.31 | 0.69 | 0.81 | -1.58 | -0.78 | -1.17 | 0.41 | 0.99 | 3.94 | 3.92 | 2.87 | 0.57 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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