Standard name | |
---|---|
Human Ortholog | |
Description | Cytoplasmic tyrosyl-tRNA synthetase; required for cytoplasmic protein synthesis; interacts with positions 34 and 35 of the tRNATyr anticodon; mutations in human ortholog YARS are associated with Charcot-Marie-Tooth (CMT) neuropathies; human ortholog YARS functionally complements the heat sensitivity of a ts allele; protein abundance increases in response to DNA replication stress |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 2 | 13 | 17 | 26 | 22 | 28 | 37 | 35 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 6 | 5 | 4 | 6 | 5 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 3 | 0 | 5 | 5 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 1 | 1 | 21 | 10 | 26 | 24 | 50 | 59 | 59 | 53 | 58 | 85 | 5 | 13 | 6 | 16 | 7 | 10 | 1 | 0 | 1 | 0 | 0 | 0 |
Cytoplasm | 201 | 385 | 551 | 369 | 474 | 534 | 467 | 474 | 426 | 406 | 390 | 397 | 352 | 283 | 316 | 536 | 395 | 398 | 193 | 374 | 539 | 206 | 281 | 235 |
Endoplasmic Reticulum | 1 | 4 | 7 | 7 | 15 | 36 | 109 | 128 | 103 | 114 | 74 | 120 | 4 | 3 | 6 | 16 | 15 | 19 | 1 | 3 | 15 | 0 | 1 | 2 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 12 | 2 | 2 | 6 | 4 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 1 | 1 | 1 | 11 | 32 | 21 | 19 | 38 | 37 | 49 | 0 | 0 | 0 | 2 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 1 | 0 | 0 | 0 | 1 | 0 | 2 | 0 | 1 | 0 | 1 | 1 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 1 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 4 | 1 | 1 | 22 | 9 | 1 | 0 | 2 | 3 | 4 | 2 | 0 | 0 | 0 | 2 | 2 | 1 |
Unique Cell Count | 203 | 387 | 558 | 381 | 495 | 592 | 614 | 664 | 579 | 569 | 567 | 614 | 354 | 285 | 324 | 548 | 399 | 405 | 197 | 383 | 566 | 217 | 295 | 253 |
Labelled Cell Count | 204 | 390 | 580 | 387 | 519 | 620 | 683 | 731 | 638 | 647 | 632 | 705 | 362 | 300 | 330 | 576 | 424 | 431 | 197 | 383 | 566 | 217 | 295 | 253 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 47.8 | 66.2 | 47.0 | 45.6 | 38.5 | 32.0 | 28.4 | 28.4 | 26.7 | 24.9 | 21.6 | 22.7 | 57.2 | 54.7 | 50.1 | 61.1 | 56.5 | 64.3 | 58.3 | 59.5 | 66.2 |
Std Deviation (1e-4) | 9.1 | 11.8 | 9.0 | 8.8 | 7.9 | 6.3 | 6.0 | 6.1 | 5.6 | 5.4 | 5.7 | 5.5 | 11.9 | 11.4 | 12.6 | 18.9 | 14.8 | 19.4 | 15.1 | 18.1 | 17.8 |
Intensity Change (Log2) | – | – | – | -0.05 | -0.29 | -0.56 | -0.73 | -0.73 | -0.82 | -0.92 | -1.13 | -1.05 | 0.28 | 0.22 | 0.09 | 0.38 | 0.26 | 0.45 | 0.31 | 0.34 | 0.49 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 3.5 | 4.0 | 4.7 | 4.6 | 5.3 | 6.1 | 5.7 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | -1.0 | 1.2 | 0.3 | 3.1 | 3.6 | 4.2 | 3.8 | 4.2 | 6.0 | -2.1 | 0.6 | -1.6 | -0.8 | -1.8 | -1.1 |
Cytoplasm | -2.0 | -3.0 | -6.3 | -11.5 | -13.0 | -12.2 | -12.8 | -13.6 | -14.8 | 1.0 | 0.7 | -1.3 | -1.2 | 0.4 | -0.6 |
Endoplasmic Reticulum | 0.7 | 2.0 | 4.3 | 9.4 | 10.0 | 9.4 | 10.2 | 7.7 | 10.1 | 0 | 0 | 0 | 1.9 | 2.5 | 3.2 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 3.2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 2.8 | 5.2 | 3.9 | 4.0 | 6.0 | 5.9 | 6.6 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 4.7 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 47.9352 | 59.3477 | 55.3505 | 52.3909 | 53.5121 | 58.6254 | 57.7046 | 76.341 | 72.5114 | 64.289 | 56.8269 | 68.7013 | 59.609 | 75.6163 | 68.0227 | 54.8805 | 62.833 | 69.3532 |
Actin | 0.0035 | 0.0004 | 0.0009 | 0.0001 | 0.0008 | 0.0007 | 0.0052 | 0.0002 | 0.001 | 0.0002 | 0.0027 | 0.0021 | 0.01 | 0.0001 | 0.0006 | 0.0493 | 0.0001 | 0.0003 |
Bud | 0.0007 | 0.0003 | 0.0006 | 0.0002 | 0.0002 | 0.0003 | 0.0018 | 0.0001 | 0.0003 | 0.0005 | 0.0018 | 0.0002 | 0.0004 | 0.0001 | 0.0008 | 0.0006 | 0.0003 | 0.0002 |
Bud Neck | 0.0009 | 0.0004 | 0.0007 | 0.0004 | 0.0005 | 0.0008 | 0.0004 | 0.0003 | 0.0003 | 0.0006 | 0.0005 | 0.0007 | 0.0006 | 0.0002 | 0.0004 | 0.0013 | 0.0003 | 0.0014 |
Bud Periphery | 0.0008 | 0.0002 | 0.0005 | 0.0001 | 0.0001 | 0.0001 | 0.0029 | 0 | 0.0002 | 0.0003 | 0.0024 | 0.0002 | 0.0006 | 0 | 0.0005 | 0.0008 | 0.0001 | 0.0002 |
Bud Site | 0.0006 | 0.0002 | 0.0004 | 0 | 0.0004 | 0 | 0.0007 | 0.0001 | 0.0001 | 0.0002 | 0.0009 | 0.0001 | 0.0007 | 0.0001 | 0.0001 | 0.0006 | 0 | 0 |
Cell Periphery | 0.0047 | 0.0016 | 0.0041 | 0.0005 | 0.0003 | 0.0002 | 0.0049 | 0.0048 | 0.0012 | 0.0185 | 0.0008 | 0.0008 | 0.0034 | 0.003 | 0.0015 | 0.0023 | 0.0002 | 0.0022 |
Cytoplasm | 0.8205 | 0.9555 | 0.9224 | 0.8867 | 0.8695 | 0.9603 | 0.8328 | 0.9685 | 0.9469 | 0.8161 | 0.8152 | 0.9134 | 0.8553 | 0.9778 | 0.9304 | 0.7676 | 0.9089 | 0.9297 |
Cytoplasmic Foci | 0.0044 | 0.0013 | 0.0023 | 0.0022 | 0.0187 | 0.004 | 0.0147 | 0.0006 | 0.004 | 0.0205 | 0.0231 | 0.0056 | 0.0049 | 0.0005 | 0.0017 | 0.0051 | 0.0025 | 0.0024 |
Eisosomes | 0.0007 | 0 | 0 | 0 | 0.0001 | 0 | 0.0001 | 0 | 0 | 0 | 0.0001 | 0 | 0.0009 | 0 | 0.0006 | 0.0004 | 0 | 0 |
Endoplasmic Reticulum | 0.0798 | 0.0281 | 0.0359 | 0.0574 | 0.0574 | 0.0158 | 0.0469 | 0.013 | 0.0121 | 0.0196 | 0.051 | 0.0369 | 0.0499 | 0.0061 | 0.0203 | 0.0253 | 0.0372 | 0.0362 |
Endosome | 0.0114 | 0.0019 | 0.0052 | 0.0058 | 0.0103 | 0.0068 | 0.0066 | 0.0008 | 0.0032 | 0.0047 | 0.0103 | 0.0064 | 0.0061 | 0.0006 | 0.0027 | 0.0059 | 0.0155 | 0.0055 |
Golgi | 0.0017 | 0.0004 | 0.0011 | 0.0003 | 0.001 | 0.002 | 0.0017 | 0.0002 | 0.0007 | 0.0003 | 0.0051 | 0.0008 | 0.0018 | 0.0001 | 0.0004 | 0.0032 | 0.0007 | 0.0006 |
Lipid Particles | 0.0047 | 0.0003 | 0.0007 | 0.0002 | 0.007 | 0 | 0.0058 | 0.001 | 0.0015 | 0.0156 | 0.0165 | 0.0015 | 0.0036 | 0.0003 | 0.0004 | 0.0213 | 0.0003 | 0.0005 |
Mitochondria | 0.0019 | 0.0001 | 0.0024 | 0.0001 | 0.0008 | 0.0013 | 0.0093 | 0.0001 | 0.0023 | 0.0013 | 0.0157 | 0.0015 | 0.009 | 0 | 0.0032 | 0.0336 | 0.0003 | 0.0006 |
None | 0.0048 | 0.0002 | 0.0002 | 0.0001 | 0.006 | 0.0002 | 0.0048 | 0.0001 | 0.0007 | 0.0033 | 0.0063 | 0.0012 | 0.003 | 0.0001 | 0.0073 | 0.0004 | 0.0005 | 0.0002 |
Nuclear Periphery | 0.0075 | 0.0011 | 0.002 | 0.0021 | 0.005 | 0.0019 | 0.0067 | 0.0016 | 0.0015 | 0.0051 | 0.0044 | 0.0038 | 0.0116 | 0.0007 | 0.0029 | 0.0054 | 0.0038 | 0.0047 |
Nucleolus | 0.0011 | 0 | 0 | 0 | 0.0012 | 0 | 0.0034 | 0 | 0.0013 | 0.0008 | 0.0014 | 0.0004 | 0.0021 | 0 | 0.0011 | 0.001 | 0 | 0 |
Nucleus | 0.0054 | 0.0008 | 0.001 | 0.001 | 0.003 | 0.0008 | 0.0052 | 0.0007 | 0.0016 | 0.0025 | 0.0031 | 0.0026 | 0.0038 | 0.0006 | 0.0024 | 0.0024 | 0.0009 | 0.0012 |
Peroxisomes | 0.0037 | 0 | 0 | 0 | 0.0003 | 0.0001 | 0.001 | 0 | 0.0017 | 0.0007 | 0.0197 | 0.0008 | 0.0037 | 0 | 0.0001 | 0.0233 | 0 | 0 |
Punctate Nuclear | 0.0021 | 0 | 0 | 0 | 0.0053 | 0 | 0.0021 | 0 | 0.0006 | 0.001 | 0.007 | 0.0029 | 0.0042 | 0 | 0.0002 | 0.0002 | 0 | 0 |
Vacuole | 0.0325 | 0.0064 | 0.0167 | 0.0385 | 0.009 | 0.0036 | 0.0378 | 0.0076 | 0.0153 | 0.0825 | 0.0089 | 0.0155 | 0.0191 | 0.0094 | 0.017 | 0.0319 | 0.0194 | 0.0115 |
Vacuole Periphery | 0.0067 | 0.0006 | 0.0028 | 0.0043 | 0.003 | 0.0011 | 0.0052 | 0.0003 | 0.0034 | 0.0057 | 0.0033 | 0.0026 | 0.0052 | 0.0003 | 0.0054 | 0.0183 | 0.009 | 0.0023 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 286.792 | 271.3575 | 267.4492 | 290.952 | 299.4024 | 258.7144 | 293.7266 | 257.6058 | 249.3664 | 267.2732 |
Translational Efficiency | 1.5428 | 1.7287 | 1.6341 | 1.4504 | 1.4093 | 1.5908 | 1.336 | 1.5148 | 1.5274 | 1.4629 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
1293 | 252 | 997 | 1069 | 1600 | 1799 | 1312 | 1396 | 2893 | 2051 | 2309 | 2465 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 3501.63 | 3949.35 | 4615.30 | 4961.20 | 3711.54 | 4177.65 | 4761.96 | 4999.90 | 3617.72 | 4149.60 | 4698.63 | 4983.12 |
Standard Deviation | 658.06 | 1072.05 | 819.91 | 1062.34 | 693.50 | 804.73 | 799.80 | 1115.29 | 685.88 | 845.49 | 811.80 | 1092.81 |
Intensity Change Log 2 | — | 0.173589 | 0.398398 | 0.502662 | — | 0.170674 | 0.359538 | 0.429881 | — | 0.172089 | 0.378533 | 0.465672 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000064 | 0.000159 | 0.000192 | 0.001145 | 0.000052 | 0.000097 | 0.000164 | 0.001361 | 0.000057 | 0.000104 | 0.000176 | 0.001268 |
Bud Neck | 0.000287 | 0.001202 | 0.002880 | 0.001435 | 0.000294 | 0.000957 | 0.002643 | 0.001592 | 0.000291 | 0.000987 | 0.002745 | 0.001524 |
Bud Site | 0.002027 | 0.009764 | 0.004891 | 0.009975 | 0.002108 | 0.002326 | 0.003794 | 0.009546 | 0.002072 | 0.003240 | 0.004268 | 0.009732 |
Cell Periphery | 0.012278 | 0.015167 | 0.021844 | 0.045917 | 0.009566 | 0.004085 | 0.014194 | 0.023160 | 0.010778 | 0.005447 | 0.017497 | 0.033029 |
Cytoplasm | 0.840962* | 0.795225* | 0.650727* | 0.683220* | 0.810055* | 0.860925* | 0.756561* | 0.731618* | 0.823868* | 0.852853* | 0.710863* | 0.710629* |
Cytoplasmic Foci | 0.005893 | 0.013022 | 0.007970 | 0.003406 | 0.008861 | 0.008304 | 0.009600 | 0.004872 | 0.007535 | 0.008884 | 0.008896 | 0.004236 |
Eisosomes | 0.000001 | 0.000002 | 0.000004 | 0.000009 | 0.000001 | 0.000002 | 0.000003 | 0.000008 | 0.000001 | 0.000002 | 0.000003 | 0.000009 |
Endoplasmic Reticulum | 0.000742 | 0.000291 | 0.000575 | 0.000209 | 0.001754 | 0.000396 | 0.000229 | 0.000298 | 0.001301 | 0.000383 | 0.000378 | 0.000259 |
Endosome | 0.000630 | 0.000858 | 0.001018 | 0.000941 | 0.000759 | 0.000770 | 0.000640 | 0.001296 | 0.000701 | 0.000781 | 0.000803 | 0.001142 |
Golgi | 0.043025 | 0.034670 | 0.026584 | 0.011737 | 0.056472 | 0.029797 | 0.021411 | 0.012721 | 0.050462 | 0.030396 | 0.023644 | 0.012294 |
Lipid Particles | 0.013590 | 0.017101 | 0.029386 | 0.021241 | 0.009314 | 0.008156 | 0.018023 | 0.022010 | 0.011225 | 0.009255 | 0.022930 | 0.021677 |
Mitochondria | 0.000069 | 0.000077 | 0.000091 | 0.000880 | 0.000107 | 0.000136 | 0.000090 | 0.000558 | 0.000090 | 0.000128 | 0.000090 | 0.000698 |
Mitotic Spindle | 0.000639 | 0.001292 | 0.001241 | 0.000337 | 0.001253 | 0.001203 | 0.001692 | 0.001058 | 0.000979 | 0.001214 | 0.001497 | 0.000746 |
None | 0.001446 | 0.003694 | 0.001089 | 0.001660 | 0.001575 | 0.001639 | 0.001717 | 0.001566 | 0.001518 | 0.001891 | 0.001446 | 0.001607 |
Nuclear Periphery | 0.000259 | 0.000304 | 0.000959 | 0.000335 | 0.000416 | 0.000284 | 0.000899 | 0.000283 | 0.000346 | 0.000287 | 0.000925 | 0.000305 |
Nuclear Periphery Foci | 0.011111 | 0.009897 | 0.015461 | 0.004190 | 0.013120 | 0.010317 | 0.014178 | 0.005734 | 0.012222 | 0.010266 | 0.014732 | 0.005064 |
Nucleolus | 0.000028 | 0.000100 | 0.000092 | 0.000170 | 0.000048 | 0.000085 | 0.000072 | 0.000130 | 0.000039 | 0.000087 | 0.000080 | 0.000148 |
Nucleus | 0.004723 | 0.014886 | 0.016589 | 0.016139 | 0.004601 | 0.012583 | 0.026581 | 0.015183 | 0.004655 | 0.012866 | 0.022266 | 0.015597 |
Peroxisomes | 0.000017 | 0.000045 | 0.000023 | 0.000113 | 0.000012 | 0.000011 | 0.000014 | 0.000090 | 0.000014 | 0.000015 | 0.000018 | 0.000100 |
Vacuole | 0.037629 | 0.063151 | 0.194927 | 0.184130 | 0.035329 | 0.044039 | 0.114923 | 0.155195 | 0.036357 | 0.046388 | 0.149468 | 0.167744 |
Vacuole Periphery | 0.024582 | 0.019093 | 0.023457 | 0.012809 | 0.044302 | 0.013887 | 0.012573 | 0.011720 | 0.035488 | 0.014527 | 0.017272 | 0.012193 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -4.32 | -6.41 | -1.89 | -1.71 | -1.57 | -2.50 | -10.80 | -1.63 | -1.56 | -1.40 | -2.89 | -11.38 | -2.37 | -2.26 | -2.01 |
Bud Neck | -3.71 | -10.99 | -8.73 | -1.35 | 6.62 | -3.24 | -10.33 | -8.84 | -3.23 | 4.48 | -3.82 | -14.97 | -12.12 | -3.29 | 7.61 |
Bud Site | -4.11 | -6.66 | -6.82 | -0.45 | -3.40 | -0.48 | -3.39 | -6.11 | -6.11 | -4.11 | -2.90 | -6.49 | -8.98 | -7.49 | -5.33 |
Cell Periphery | -1.14 | -5.07 | -11.72 | -8.36 | -8.12 | 6.72 | -4.04 | -6.99 | -11.27 | -3.90 | 7.20 | -6.40 | -13.33 | -17.61 | -8.69 |
Cytoplasm | 2.69 | 17.04 | 14.16 | 6.11 | -3.96 | -5.35 | 7.69 | 9.03 | 14.75 | 1.03 | -3.46 | 17.14 | 16.10 | 18.89 | -1.98 |
Cytoplasmic Foci | -4.23 | -5.16 | 3.89 | 5.52 | 8.37 | 0.19 | -3.44 | 5.09 | 5.08 | 7.75 | -2.83 | -5.81 | 6.31 | 8.11 | 11.10 |
Eisosomes | -2.49 | -10.06 | -3.33 | -2.87 | -1.91 | -1.19 | -10.58 | -2.55 | -1.89 | -1.42 | -1.25 | -14.56 | -3.99 | -2.92 | -2.25 |
Endoplasmic Reticulum | 4.12 | 1.39 | 6.14 | 1.19 | 3.17 | 3.92 | 4.45 | 4.27 | 1.51 | -0.66 | 4.61 | 4.68 | 5.39 | 2.26 | 2.15 |
Endosome | -1.62 | -5.15 | -0.61 | 0.00 | 0.69 | -0.55 | 0.48 | -1.63 | -1.44 | -1.75 | -1.58 | -3.38 | -1.55 | -1.05 | -0.64 |
Golgi | 1.70 | 6.47 | 12.47 | 5.04 | 8.42 | 9.29 | 13.50 | 17.28 | 10.19 | 6.69 | 8.97 | 14.56 | 21.24 | 11.78 | 10.64 |
Lipid Particles | -1.35 | -7.06 | -4.12 | -1.22 | 3.61 | 0.88 | -7.66 | -7.33 | -7.91 | -1.26 | 1.76 | -10.13 | -8.22 | -9.33 | 1.67 |
Mitochondria | -0.61 | -3.50 | -2.12 | -2.10 | -2.02 | -0.77 | 0.25 | -1.86 | -1.69 | -1.88 | -1.19 | -1.48 | -2.82 | -2.58 | -2.74 |
Mitotic Spindle | -2.69 | -4.54 | 2.16 | 3.60 | 5.49 | -0.05 | -1.41 | 0.36 | 0.52 | 1.74 | -1.24 | -2.56 | 0.73 | 2.06 | 3.17 |
None | -2.78 | 3.21 | -0.38 | 2.27 | -1.26 | -0.59 | -0.67 | 0.37 | 0.62 | 0.74 | -2.63 | 0.66 | -0.06 | 1.32 | -0.37 |
Nuclear Periphery | -1.08 | -11.28 | -2.98 | -0.65 | 10.27 | 2.68 | -8.06 | 3.00 | 0.26 | 10.63 | 1.47 | -13.24 | 1.35 | -0.41 | 14.70 |
Nuclear Periphery Foci | 0.74 | -3.82 | 7.50 | 4.59 | 12.70 | 2.61 | -1.31 | 8.78 | 6.15 | 8.77 | 2.12 | -3.44 | 11.48 | 8.19 | 14.41 |
Nucleolus | -3.99 | -11.20 | -5.13 | -2.24 | -2.30 | -1.08 | -2.54 | -5.40 | -1.23 | -5.25 | -1.59 | -6.58 | -7.38 | -1.81 | -4.18 |
Nucleus | -4.67 | -10.48 | -9.03 | -1.19 | 1.66 | -10.15 | -16.66 | -11.15 | -3.44 | 7.07 | -11.10 | -19.54 | -14.32 | -3.91 | 6.64 |
Peroxisomes | -2.11 | -2.29 | -3.30 | -1.99 | -2.87 | 0.03 | -2.22 | -4.65 | -4.65 | -4.34 | -0.74 | -3.05 | -5.42 | -5.25 | -4.89 |
Vacuole | -3.05 | -20.72 | -21.41 | -11.81 | 2.47 | -3.29 | -16.63 | -21.49 | -19.07 | -4.99 | -4.07 | -26.12 | -30.23 | -25.51 | -1.73 |
Vacuole Periphery | 1.49 | 0.96 | 6.36 | 1.91 | 6.01 | 11.77 | 12.59 | 13.07 | 2.68 | 1.26 | 11.79 | 10.93 | 14.40 | 2.96 | 5.32 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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