Standard name | |
---|---|
Human Ortholog | |
Description | Protein kinase involved in the cell cycle checkpoint into anaphase; in complex with Mad1p and Bub3p, prevents progression into anaphase in presence of spindle damage; Cdc28p-mediated phosphorylation at Bub1p-T566 is important for degradation in anaphase and adaptation of checkpoint to prolonged mitotic arrest; associates with centromere DNA via Skp1p; involved in Sgo1p relocalization in response to sister kinetochore tension; paralog MAD3 arose from whole genome duplication |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 5 | 3 | 2 | 3 | 6 |
Bud | 0 | 0 | 7 | 11 | 5 | 9 | 22 | 26 | 8 | 24 | 8 | 13 | 2 | 0 | 9 | 0 | 0 | 0 | 4 | 2 | 2 | 4 | 9 | 8 |
Bud Neck | 8 | 0 | 34 | 20 | 42 | 38 | 46 | 35 | 11 | 20 | 11 | 18 | 0 | 4 | 5 | 0 | 0 | 0 | 3 | 2 | 0 | 2 | 0 | 4 |
Bud Site | 2 | 0 | 4 | 5 | 6 | 10 | 17 | 20 | 12 | 16 | 3 | 9 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 1 | 1 | 2 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 1 |
Cytoplasm | 164 | 91 | 424 | 235 | 294 | 325 | 437 | 508 | 288 | 323 | 205 | 216 | 156 | 156 | 162 | 69 | 44 | 49 | 109 | 55 | 195 | 108 | 148 | 173 |
Endoplasmic Reticulum | 2 | 3 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 3 | 0 | 0 | 1 | 0 | 18 | 9 | 11 | 1 | 2 | 3 | 2 | 1 | 3 |
Endosome | 8 | 14 | 1 | 0 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 59 | 46 | 24 | 59 | 24 | 30 | 13 | 6 | 11 | 2 | 3 | 3 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 1 | 3 | 4 | 0 | 0 | 3 | 0 |
Mitochondria | 2 | 25 | 44 | 50 | 54 | 121 | 218 | 264 | 267 | 262 | 213 | 276 | 2 | 1 | 6 | 10 | 7 | 5 | 25 | 19 | 54 | 6 | 12 | 20 |
Nucleus | 3 | 4 | 2 | 6 | 2 | 2 | 3 | 6 | 3 | 2 | 6 | 5 | 3 | 3 | 3 | 37 | 39 | 27 | 14 | 13 | 15 | 8 | 6 | 2 |
Nuclear Periphery | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 |
Nucleolus | 3 | 1 | 0 | 0 | 1 | 2 | 1 | 3 | 2 | 4 | 2 | 4 | 1 | 0 | 0 | 30 | 15 | 13 | 4 | 1 | 3 | 0 | 0 | 0 |
Peroxisomes | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 3 | 0 | 1 | 0 | 2 | 1 | 1 | 0 | 1 | 0 | 0 | 2 |
SpindlePole | 44 | 20 | 63 | 50 | 92 | 64 | 103 | 107 | 36 | 69 | 16 | 39 | 50 | 35 | 17 | 77 | 54 | 24 | 92 | 66 | 132 | 3 | 11 | 11 |
Vac/Vac Membrane | 50 | 25 | 1 | 1 | 2 | 2 | 2 | 6 | 5 | 3 | 4 | 11 | 21 | 8 | 7 | 78 | 45 | 33 | 38 | 44 | 43 | 33 | 30 | 26 |
Unique Cell Count | 258 | 168 | 533 | 329 | 441 | 472 | 698 | 771 | 497 | 553 | 381 | 455 | 261 | 233 | 222 | 283 | 186 | 144 | 329 | 235 | 506 | 181 | 241 | 273 |
Labelled Cell Count | 287 | 187 | 583 | 378 | 501 | 573 | 849 | 976 | 634 | 725 | 472 | 591 | 297 | 254 | 235 | 381 | 239 | 194 | 329 | 235 | 506 | 181 | 241 | 273 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 4.9 | 3.9 | 3.0 | 3.0 | 3.1 | 2.8 | 2.6 | 2.8 | 2.3 | 2.3 | 2.4 | 2.2 | 4.4 | 4.5 | 3.9 | 6.0 | 6.5 | 6.4 | 4.6 | 4.6 | 5.0 |
Std Deviation (1e-4) | 0.7 | 1.0 | 0.6 | 1.2 | 0.8 | 0.6 | 0.8 | 0.8 | 0.6 | 0.7 | 0.6 | 0.6 | 1.4 | 1.1 | 1.0 | 1.1 | 1.7 | 2.0 | 1.7 | 1.7 | 1.7 |
Intensity Change (Log2) | – | – | – | -0.03 | 0.04 | -0.13 | -0.23 | -0.1 | -0.4 | -0.38 | -0.34 | -0.44 | 0.55 | 0.57 | 0.37 | 0.99 | 1.09 | 1.08 | 0.62 | 0.61 | 0.73 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Endosome | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Golgi | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | 12.6 | 11.5 | 11.1 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 1.208 | 0.6708 | 0.5 | 0.5133 | 0.4864 | 0.4306 | 4.2425 | 3.7363 | 3.9975 | 3.7575 | 3.6933 | 3.9298 | 3.5955 | 3.7032 | 3.5398 | 3.5135 | 3.2259 | 3.9099 |
Actin | 0.0524 | 0.0062 | 0.0111 | 0.0008 | 0.0028 | 0.0006 | 0.0135 | 0.0005 | 0.0163 | 0.0019 | 0.0011 | 0.0077 | 0.0059 | 0.0025 | 0.0014 | 0.0009 | 0.0006 | 0.0019 |
Bud | 0.0135 | 0.0281 | 0.0139 | 0.0035 | 0.0518 | 0.0103 | 0.0014 | 0.0065 | 0.0028 | 0.0033 | 0.0021 | 0.001 | 0.0034 | 0.0116 | 0.002 | 0.0078 | 0.0002 | 0.0004 |
Bud Neck | 0.005 | 0.0019 | 0.0072 | 0.0021 | 0.0023 | 0.0057 | 0.0092 | 0.0018 | 0.0015 | 0.0237 | 0.0001 | 0.0024 | 0.0069 | 0.0015 | 0.0033 | 0.0004 | 0.0004 | 0.0065 |
Bud Periphery | 0.0077 | 0.0068 | 0.005 | 0.0012 | 0.0207 | 0.0035 | 0.0008 | 0.0018 | 0.0018 | 0.0023 | 0.001 | 0.001 | 0.0017 | 0.0023 | 0.001 | 0.0029 | 0.0001 | 0.0005 |
Bud Site | 0.0183 | 0.0282 | 0.0101 | 0.0004 | 0.0044 | 0.0005 | 0.002 | 0.0157 | 0.0046 | 0.0004 | 0.0002 | 0.0005 | 0.0028 | 0.0156 | 0.0083 | 0.001 | 0.0002 | 0.0004 |
Cell Periphery | 0.0009 | 0.0007 | 0.0004 | 0.0001 | 0.0005 | 0.0002 | 0.0001 | 0.0002 | 0.0003 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0003 | 0.0001 | 0.0001 | 0.0001 | 0.0001 |
Cytoplasm | 0.094 | 0.1649 | 0.1098 | 0.1552 | 0.1422 | 0.2273 | 0.0625 | 0.0793 | 0.0583 | 0.0819 | 0.0661 | 0.0874 | 0.0603 | 0.1035 | 0.0723 | 0.0937 | 0.127 | 0.1071 |
Cytoplasmic Foci | 0.183 | 0.062 | 0.1159 | 0.0203 | 0.0283 | 0.0796 | 0.0588 | 0.0344 | 0.041 | 0.0112 | 0.0053 | 0.0499 | 0.0726 | 0.0448 | 0.0453 | 0.0184 | 0.0254 | 0.0377 |
Eisosomes | 0.0005 | 0.0005 | 0.0002 | 0.0001 | 0.0001 | 0 | 0.0004 | 0.0001 | 0.0002 | 0.0001 | 0.0002 | 0.0001 | 0.0003 | 0.0002 | 0.0002 | 0.0001 | 0.0003 | 0.0002 |
Endoplasmic Reticulum | 0.0108 | 0.0029 | 0.0043 | 0.0023 | 0.0027 | 0.0023 | 0.0037 | 0.0016 | 0.0019 | 0.0011 | 0.0009 | 0.0014 | 0.0012 | 0.002 | 0.0012 | 0.0025 | 0.0016 | 0.0013 |
Endosome | 0.0307 | 0.0059 | 0.0084 | 0.0057 | 0.0079 | 0.0052 | 0.0191 | 0.0057 | 0.0051 | 0.002 | 0.0023 | 0.0047 | 0.008 | 0.0052 | 0.0061 | 0.004 | 0.0034 | 0.0068 |
Golgi | 0.0054 | 0.0009 | 0.001 | 0.0003 | 0.0005 | 0.0002 | 0.0045 | 0.0004 | 0.0016 | 0.0002 | 0.0006 | 0.0005 | 0.0007 | 0.0006 | 0.0004 | 0.0002 | 0.0002 | 0.0003 |
Lipid Particles | 0.0275 | 0.0033 | 0.0036 | 0.0007 | 0.001 | 0.0024 | 0.0155 | 0.0027 | 0.0033 | 0.0003 | 0.0192 | 0.0013 | 0.0045 | 0.0027 | 0.0024 | 0.0012 | 0.0021 | 0.0025 |
Mitochondria | 0.0098 | 0.0021 | 0.0017 | 0.0013 | 0.0073 | 0.0009 | 0.0017 | 0.0073 | 0.0028 | 0.0009 | 0.0314 | 0.0007 | 0.001 | 0.0015 | 0.0008 | 0.0019 | 0.0007 | 0.0006 |
None | 0.3075 | 0.4341 | 0.274 | 0.533 | 0.4874 | 0.4066 | 0.5731 | 0.6072 | 0.4797 | 0.6548 | 0.7827 | 0.607 | 0.6195 | 0.5348 | 0.4347 | 0.6562 | 0.6951 | 0.6283 |
Nuclear Periphery | 0.031 | 0.0056 | 0.0076 | 0.0155 | 0.0219 | 0.0069 | 0.0196 | 0.0041 | 0.0092 | 0.0048 | 0.0146 | 0.0051 | 0.0025 | 0.0067 | 0.0054 | 0.0143 | 0.0048 | 0.0037 |
Nucleolus | 0.0077 | 0.0088 | 0.0076 | 0.0043 | 0.0107 | 0.0055 | 0.0062 | 0.0106 | 0.0084 | 0.0019 | 0.0023 | 0.0024 | 0.0029 | 0.0109 | 0.0075 | 0.0021 | 0.0016 | 0.0024 |
Nucleus | 0.0391 | 0.0909 | 0.0989 | 0.0998 | 0.1015 | 0.102 | 0.0406 | 0.0593 | 0.0732 | 0.0598 | 0.044 | 0.0643 | 0.0359 | 0.0842 | 0.0908 | 0.1024 | 0.055 | 0.0647 |
Peroxisomes | 0.0119 | 0.0011 | 0.0018 | 0.0006 | 0.0011 | 0.0007 | 0.0063 | 0.0016 | 0.0065 | 0.0004 | 0.0032 | 0.0011 | 0.0058 | 0.0105 | 0.0014 | 0.0008 | 0.0009 | 0.0014 |
Punctate Nuclear | 0.1361 | 0.1426 | 0.3149 | 0.1512 | 0.099 | 0.1372 | 0.1581 | 0.1569 | 0.2803 | 0.1475 | 0.0207 | 0.1606 | 0.1627 | 0.1557 | 0.3139 | 0.0867 | 0.0794 | 0.1317 |
Vacuole | 0.0052 | 0.0023 | 0.0021 | 0.0013 | 0.0047 | 0.002 | 0.0019 | 0.002 | 0.0009 | 0.001 | 0.0008 | 0.0006 | 0.0011 | 0.0026 | 0.0012 | 0.0018 | 0.0009 | 0.0011 |
Vacuole Periphery | 0.002 | 0.0003 | 0.0004 | 0.0005 | 0.0012 | 0.0004 | 0.0009 | 0.0002 | 0.0003 | 0.0002 | 0.0011 | 0.0002 | 0.0001 | 0.0004 | 0.0002 | 0.0007 | 0.0002 | 0.0002 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 8.6372 | 6.1126 | 3.1034 | 3.0392 | 6.9991 | 8.2728 | 5.3672 | 2.9639 | 1.719 | 10.8485 |
Translational Efficiency | – | – | – | – | – | – | – | – | – | – |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✔ |
Translational Efficiency | – |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
571 | 1525 | 1096 | 658 | 1504 | 1322 | 238 | 1427 | 2075 | 2847 | 1334 | 2085 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 602.00 | 694.41 | 767.69 | 757.16 | 651.32 | 678.93 | 843.69 | 772.27 | 637.75 | 687.22 | 781.25 | 767.50 |
Standard Deviation | 69.62 | 101.52 | 94.09 | 121.03 | 77.16 | 100.57 | 96.61 | 114.81 | 78.32 | 101.37 | 98.92 | 117.02 |
Intensity Change Log 2 | — | 0.206024 | 0.350760 | 0.330835 | — | 0.059896 | 0.373346 | 0.245739 | — | 0.131934 | 0.362542 | 0.287239 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000032 | 0.000205 | 0.000371 | 0.003431 | 0.000057 | 0.000413 | 0.000693 | 0.000600 | 0.000050 | 0.000302 | 0.000428 | 0.001493 |
Bud Neck | 0.002436 | 0.011228 | 0.006355 | 0.010426 | 0.011183 | 0.022523 | 0.001888 | 0.018355 | 0.008776 | 0.016473 | 0.005558 | 0.015853 |
Bud Site | 0.000569 | 0.005850 | 0.010246 | 0.021842 | 0.001356 | 0.012995 | 0.002863 | 0.031840 | 0.001140 | 0.009168 | 0.008929 | 0.028685 |
Cell Periphery | 0.000135 | 0.000124 | 0.000238 | 0.000290 | 0.000115 | 0.000181 | 0.000151 | 0.000271 | 0.000121 | 0.000150 | 0.000222 | 0.000277 |
Cytoplasm | 0.565461* | 0.394071* | 0.155487* | 0.322032* | 0.393875* | 0.334641* | 0.109408* | 0.281248* | 0.441092* | 0.366474* | 0.147266* | 0.294119* |
Cytoplasmic Foci | 0.094660 | 0.104850 | 0.006777 | 0.026116 | 0.131225 | 0.237072* | 0.005408 | 0.021073 | 0.121163 | 0.166247 | 0.006533 | 0.022665 |
Eisosomes | 0.000062 | 0.000060 | 0.000035 | 0.000082 | 0.000067 | 0.000115 | 0.000070 | 0.000052 | 0.000066 | 0.000086 | 0.000041 | 0.000061 |
Endoplasmic Reticulum | 0.000231 | 0.001192 | 0.002550 | 0.002586 | 0.000986 | 0.000868 | 0.007394 | 0.003127 | 0.000779 | 0.001042 | 0.003414 | 0.002957 |
Endosome | 0.002031 | 0.007125 | 0.001452 | 0.013992 | 0.004652 | 0.017580 | 0.002673 | 0.014504 | 0.003931 | 0.011980 | 0.001670 | 0.014342 |
Golgi | 0.000262 | 0.001241 | 0.000179 | 0.003041 | 0.002004 | 0.005016 | 0.000125 | 0.003638 | 0.001525 | 0.002994 | 0.000169 | 0.003450 |
Lipid Particles | 0.002710 | 0.006844 | 0.001190 | 0.001901 | 0.011474 | 0.017856 | 0.002073 | 0.002278 | 0.009062 | 0.011957 | 0.001347 | 0.002159 |
Mitochondria | 0.000489 | 0.005839 | 0.002479 | 0.001716 | 0.005312 | 0.015792 | 0.000836 | 0.007733 | 0.003985 | 0.010461 | 0.002186 | 0.005834 |
Mitotic Spindle | 0.000113 | 0.018509 | 0.010104 | 0.065698 | 0.005908 | 0.026169 | 0.013861 | 0.151823* | 0.004313 | 0.022066 | 0.010774 | 0.124643* |
None | 0.025217 | 0.013817 | 0.011066 | 0.026611 | 0.010234 | 0.010409 | 0.009132 | 0.013546 | 0.014357 | 0.012234 | 0.010721 | 0.017669 |
Nuclear Periphery | 0.000221 | 0.001345 | 0.001478 | 0.002216 | 0.001690 | 0.001202 | 0.001899 | 0.003254 | 0.001286 | 0.001279 | 0.001553 | 0.002926 |
Nuclear Periphery Foci | 0.000797 | 0.002221 | 0.000866 | 0.003184 | 0.001377 | 0.002002 | 0.002447 | 0.003580 | 0.001217 | 0.002119 | 0.001148 | 0.003455 |
Nucleolus | 0.003704 | 0.009325 | 0.006955 | 0.006392 | 0.010854 | 0.014168 | 0.005925 | 0.008381 | 0.008886 | 0.011574 | 0.006771 | 0.007753 |
Nucleus | 0.291565* | 0.388158* | 0.769138* | 0.424366* | 0.390741* | 0.232026* | 0.803973* | 0.359476* | 0.363450* | 0.315659* | 0.775353* | 0.379954* |
Peroxisomes | 0.000678 | 0.007059 | 0.000825 | 0.003428 | 0.006759 | 0.025998 | 0.000467 | 0.003270 | 0.005085 | 0.015853 | 0.000761 | 0.003320 |
Vacuole | 0.008484 | 0.020002 | 0.011524 | 0.058550 | 0.009676 | 0.021399 | 0.028510 | 0.060585 | 0.009348 | 0.020651 | 0.014555 | 0.059943 |
Vacuole Periphery | 0.000142 | 0.000934 | 0.000686 | 0.002101 | 0.000455 | 0.001574 | 0.000202 | 0.011366 | 0.000369 | 0.001232 | 0.000600 | 0.008442 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -9.20 | -10.14 | -2.83 | -2.45 | -1.75 | -7.19 | -7.47 | -16.97 | -7.37 | 3.23 | -10.83 | -12.26 | -5.53 | -4.15 | -1.48 |
Bud Neck | -7.14 | -6.95 | -6.38 | -0.73 | -0.19 | -5.42 | 3.03 | -5.78 | 0.58 | -7.98 | -6.64 | -2.59 | -7.71 | -1.28 | -4.73 |
Bud Site | -6.79 | -7.76 | -7.89 | -4.96 | -2.22 | -6.75 | -4.57 | -12.60 | -6.81 | -7.72 | -9.15 | -8.10 | -14.80 | -9.14 | -6.02 |
Cell Periphery | 1.09 | -3.66 | -3.51 | -4.03 | 1.00 | -3.17 | -4.72 | -5.44 | -1.05 | 0.44 | -2.71 | -4.90 | -6.52 | -3.65 | 1.44 |
Cytoplasm | 14.26 | 33.09 | 22.02 | 11.25 | -7.67 | 6.28 | 24.97 | 18.83 | 11.47 | -9.99 | 10.43 | 36.51 | 26.63 | 17.73 | -10.40 |
Cytoplasmic Foci | -1.66 | 15.36 | 13.15 | 20.03 | -4.11 | -11.36 | 26.48 | 22.69 | 32.99 | -7.83 | -7.00 | 29.91 | 26.10 | 36.69 | -7.70 |
Eisosomes | -0.96 | 0.24 | -6.14 | -5.76 | -6.10 | -8.21 | -4.80 | -4.70 | 3.17 | 2.87 | -6.00 | -1.52 | -7.23 | -2.21 | -4.37 |
Endoplasmic Reticulum | -9.13 | -14.25 | -14.11 | -11.23 | -3.96 | 0.70 | -9.78 | -14.32 | -15.97 | 6.04 | -2.70 | -14.66 | -19.41 | -19.00 | 0.39 |
Endosome | -5.50 | -0.48 | -3.75 | -0.34 | -3.88 | -9.64 | -0.58 | -5.06 | 4.72 | -2.61 | -10.46 | 1.84 | -6.19 | 2.61 | -7.34 |
Golgi | -6.16 | -1.46 | -2.51 | 0.21 | -2.24 | -3.49 | 3.28 | 0.39 | 3.88 | -2.98 | -3.27 | 3.22 | -0.25 | 3.08 | -3.71 |
Lipid Particles | -6.34 | 3.72 | 2.04 | 8.22 | -2.58 | -3.26 | 8.06 | 8.94 | 10.24 | 0.15 | -2.40 | 10.28 | 9.12 | 12.39 | -4.16 |
Mitochondria | -7.60 | -4.00 | -4.98 | 6.02 | 2.01 | -5.30 | 7.44 | 1.53 | 5.88 | -3.75 | -6.07 | 3.17 | 1.34 | 6.64 | -1.42 |
Mitotic Spindle | -10.03 | -9.09 | -12.79 | -9.48 | -8.81 | -7.81 | -3.60 | -23.53 | -18.83 | -12.20 | -11.18 | -8.30 | -26.53 | -21.43 | -19.34 |
None | 4.27 | 5.79 | 3.43 | 0.06 | -1.52 | -1.01 | 1.93 | 1.59 | 2.25 | -0.76 | 1.36 | 4.12 | 3.33 | 2.29 | -0.48 |
Nuclear Periphery | -8.26 | -19.41 | -12.38 | -8.24 | -6.37 | 2.81 | -5.66 | -11.68 | -12.84 | -4.96 | 0.10 | -8.29 | -15.79 | -15.32 | -10.96 |
Nuclear Periphery Foci | -4.14 | -5.33 | -5.44 | -2.56 | -0.62 | -0.70 | -3.35 | -8.01 | -7.91 | 0.33 | -2.79 | -5.46 | -9.39 | -7.66 | -2.27 |
Nucleolus | -7.08 | -7.63 | -2.71 | 3.09 | 4.50 | 0.09 | 0.81 | -1.21 | -1.36 | -1.52 | -1.98 | -3.11 | -1.78 | 0.03 | 1.67 |
Nucleus | -5.64 | -30.90 | -6.94 | -2.82 | 19.26 | 15.55 | -16.01 | 3.69 | -10.35 | 17.57 | 6.85 | -34.44 | -0.37 | -6.54 | 32.02 |
Peroxisomes | -10.35 | -0.69 | -2.71 | 7.29 | -2.17 | -10.09 | 7.23 | 3.46 | 13.08 | -5.07 | -10.51 | 6.65 | 2.91 | 14.21 | -4.96 |
Vacuole | -6.53 | -4.09 | -14.00 | -12.27 | -12.92 | -8.18 | -6.32 | -18.07 | -15.42 | -4.12 | -10.13 | -7.37 | -22.82 | -19.56 | -17.60 |
Vacuole Periphery | -4.49 | -3.08 | -3.09 | -0.34 | -1.06 | -4.76 | 3.43 | -3.76 | -2.64 | -3.94 | -5.85 | -1.58 | -4.12 | -2.87 | -3.71 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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