Standard name
Human Ortholog
Description Protein kinase involved in the cell cycle checkpoint into anaphase; in complex with Mad1p and Bub3p, prevents progression into anaphase in presence of spindle damage; Cdc28p-mediated phosphorylation at Bub1p-T566 is important for degradation in anaphase and adaptation of checkpoint to prolonged mitotic arrest; associates with centromere DNA via Skp1p; involved in Sgo1p relocalization in response to sister kinetochore tension; paralog MAD3 arose from whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0.06 0.06 0.1 0.08 0.07 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.64 0.54 0.8 0.71 0.67 0.69 0.63 0.66 0.58 0.58 0.54 0.47 0.6 0.67 0.73 0.24 0.24 0.34 0.33 0.24 0.39 0.6 0.62 0.64
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.05 0.08 0 0 0 0 0 0
Endosome 0 0.08 0 0 0 0 0 0 0 0 0 0 0.23 0.2 0.11 0.21 0.13 0.21 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0.15 0.08 0.15 0.12 0.26 0.31 0.34 0.54 0.47 0.56 0.61 0 0 0 0 0 0 0.08 0.08 0.11 0 0.05 0.07
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.13 0.21 0.19 0 0.06 0 0.05 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.11 0.08 0.09 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0.17 0.12 0.12 0.15 0.21 0.14 0.15 0.14 0.07 0.12 0 0.09 0.19 0.15 0.08 0.27 0.29 0.17 0.28 0.28 0.26 0 0.05 0
Vac/Vac Membrane 0.19 0.15 0 0 0 0 0 0 0 0 0 0 0.08 0 0 0.28 0.24 0.23 0.12 0.19 0.09 0.19 0.13 0.1
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 5 3 2 3 6
Bud 0 0 7 11 5 9 22 26 8 24 8 13 2 0 9 0 0 0 4 2 2 4 9 8
Bud Neck 8 0 34 20 42 38 46 35 11 20 11 18 0 4 5 0 0 0 3 2 0 2 0 4
Bud Site 2 0 4 5 6 10 17 20 12 16 3 9 0 0 0 0 0 0 – – – – – –
Cell Periphery 1 1 2 0 0 0 0 0 2 0 1 0 0 0 1 0 0 0 0 0 2 0 0 1
Cytoplasm 164 91 424 235 294 325 437 508 288 323 205 216 156 156 162 69 44 49 109 55 195 108 148 173
Endoplasmic Reticulum 2 3 1 0 0 0 0 1 0 0 3 0 0 1 0 18 9 11 1 2 3 2 1 3
Endosome 8 14 1 0 3 0 0 0 0 0 0 0 59 46 24 59 24 30 13 6 11 2 3 3
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 0 1 3 4 0 0 3 0
Mitochondria 2 25 44 50 54 121 218 264 267 262 213 276 2 1 6 10 7 5 25 19 54 6 12 20
Nucleus 3 4 2 6 2 2 3 6 3 2 6 5 3 3 3 37 39 27 14 13 15 8 6 2
Nuclear Periphery 0 1 0 0 0 0 0 0 0 1 0 0 0 0 0 1 0 0 1 1 0 0 0 0
Nucleolus 3 1 0 0 1 2 1 3 2 4 2 4 1 0 0 30 15 13 4 1 3 0 0 0
Peroxisomes 0 1 0 0 0 0 0 0 0 1 0 0 3 0 1 0 2 1 1 0 1 0 0 2
SpindlePole 44 20 63 50 92 64 103 107 36 69 16 39 50 35 17 77 54 24 92 66 132 3 11 11
Vac/Vac Membrane 50 25 1 1 2 2 2 6 5 3 4 11 21 8 7 78 45 33 38 44 43 33 30 26
Unique Cell Count 258 168 533 329 441 472 698 771 497 553 381 455 261 233 222 283 186 144 329 235 506 181 241 273
Labelled Cell Count 287 187 583 378 501 573 849 976 634 725 472 591 297 254 235 381 239 194 329 235 506 181 241 273


Microtubule, Nucleus, SpindlePole

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.9 3.9 3.0 3.0 3.1 2.8 2.6 2.8 2.3 2.3 2.4 2.2 4.4 4.5 3.9 6.0 6.5 6.4 4.6 4.6 5.0
Std Deviation (1e-4) 0.7 1.0 0.6 1.2 0.8 0.6 0.8 0.8 0.6 0.7 0.6 0.6 1.4 1.1 1.0 1.1 1.7 2.0 1.7 1.7 1.7
Intensity Change (Log2) – – – -0.03 0.04 -0.13 -0.23 -0.1 -0.4 -0.38 -0.34 -0.44 0.55 0.57 0.37 0.99 1.09 1.08 0.62 0.61 0.73


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – 0 0 0
Bud Neck – – – – – – – – – – – – 0 0 0
Bud Site – – – – – – – – – – – – 0 0 0
Cell Periphery – – – – – – – – – – – – 0 0 0
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – 0 0 0
Endosome – – – – – – – – – – – – 0 0 0
Golgi – – – – – – – – – – – – 0 0 0
Mitochondria – – – – – – – – – – – – 0 0 0
Nuclear Periphery – – – – – – – – – – – – 0 0 0
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – 0 0 0
Peroxisomes – – – – – – – – – – – – 0 0 0
SpindlePole – – – – – – – – – – – – 0 0 0
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – 0 0 0
Cytoplasm – – – – – – – – – – – – 0 0 0
Nucleus – – – – – – – – – – – – 0 0 0
Vacuole – – – – – – – – – – – – 12.6 11.5 11.1

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 1.208 0.6708 0.5 0.5133 0.4864 0.4306 4.2425 3.7363 3.9975 3.7575 3.6933 3.9298 3.5955 3.7032 3.5398 3.5135 3.2259 3.9099
Actin 0.0524 0.0062 0.0111 0.0008 0.0028 0.0006 0.0135 0.0005 0.0163 0.0019 0.0011 0.0077 0.0059 0.0025 0.0014 0.0009 0.0006 0.0019
Bud 0.0135 0.0281 0.0139 0.0035 0.0518 0.0103 0.0014 0.0065 0.0028 0.0033 0.0021 0.001 0.0034 0.0116 0.002 0.0078 0.0002 0.0004
Bud Neck 0.005 0.0019 0.0072 0.0021 0.0023 0.0057 0.0092 0.0018 0.0015 0.0237 0.0001 0.0024 0.0069 0.0015 0.0033 0.0004 0.0004 0.0065
Bud Periphery 0.0077 0.0068 0.005 0.0012 0.0207 0.0035 0.0008 0.0018 0.0018 0.0023 0.001 0.001 0.0017 0.0023 0.001 0.0029 0.0001 0.0005
Bud Site 0.0183 0.0282 0.0101 0.0004 0.0044 0.0005 0.002 0.0157 0.0046 0.0004 0.0002 0.0005 0.0028 0.0156 0.0083 0.001 0.0002 0.0004
Cell Periphery 0.0009 0.0007 0.0004 0.0001 0.0005 0.0002 0.0001 0.0002 0.0003 0.0001 0.0001 0.0001 0.0001 0.0003 0.0001 0.0001 0.0001 0.0001
Cytoplasm 0.094 0.1649 0.1098 0.1552 0.1422 0.2273 0.0625 0.0793 0.0583 0.0819 0.0661 0.0874 0.0603 0.1035 0.0723 0.0937 0.127 0.1071
Cytoplasmic Foci 0.183 0.062 0.1159 0.0203 0.0283 0.0796 0.0588 0.0344 0.041 0.0112 0.0053 0.0499 0.0726 0.0448 0.0453 0.0184 0.0254 0.0377
Eisosomes 0.0005 0.0005 0.0002 0.0001 0.0001 0 0.0004 0.0001 0.0002 0.0001 0.0002 0.0001 0.0003 0.0002 0.0002 0.0001 0.0003 0.0002
Endoplasmic Reticulum 0.0108 0.0029 0.0043 0.0023 0.0027 0.0023 0.0037 0.0016 0.0019 0.0011 0.0009 0.0014 0.0012 0.002 0.0012 0.0025 0.0016 0.0013
Endosome 0.0307 0.0059 0.0084 0.0057 0.0079 0.0052 0.0191 0.0057 0.0051 0.002 0.0023 0.0047 0.008 0.0052 0.0061 0.004 0.0034 0.0068
Golgi 0.0054 0.0009 0.001 0.0003 0.0005 0.0002 0.0045 0.0004 0.0016 0.0002 0.0006 0.0005 0.0007 0.0006 0.0004 0.0002 0.0002 0.0003
Lipid Particles 0.0275 0.0033 0.0036 0.0007 0.001 0.0024 0.0155 0.0027 0.0033 0.0003 0.0192 0.0013 0.0045 0.0027 0.0024 0.0012 0.0021 0.0025
Mitochondria 0.0098 0.0021 0.0017 0.0013 0.0073 0.0009 0.0017 0.0073 0.0028 0.0009 0.0314 0.0007 0.001 0.0015 0.0008 0.0019 0.0007 0.0006
None 0.3075 0.4341 0.274 0.533 0.4874 0.4066 0.5731 0.6072 0.4797 0.6548 0.7827 0.607 0.6195 0.5348 0.4347 0.6562 0.6951 0.6283
Nuclear Periphery 0.031 0.0056 0.0076 0.0155 0.0219 0.0069 0.0196 0.0041 0.0092 0.0048 0.0146 0.0051 0.0025 0.0067 0.0054 0.0143 0.0048 0.0037
Nucleolus 0.0077 0.0088 0.0076 0.0043 0.0107 0.0055 0.0062 0.0106 0.0084 0.0019 0.0023 0.0024 0.0029 0.0109 0.0075 0.0021 0.0016 0.0024
Nucleus 0.0391 0.0909 0.0989 0.0998 0.1015 0.102 0.0406 0.0593 0.0732 0.0598 0.044 0.0643 0.0359 0.0842 0.0908 0.1024 0.055 0.0647
Peroxisomes 0.0119 0.0011 0.0018 0.0006 0.0011 0.0007 0.0063 0.0016 0.0065 0.0004 0.0032 0.0011 0.0058 0.0105 0.0014 0.0008 0.0009 0.0014
Punctate Nuclear 0.1361 0.1426 0.3149 0.1512 0.099 0.1372 0.1581 0.1569 0.2803 0.1475 0.0207 0.1606 0.1627 0.1557 0.3139 0.0867 0.0794 0.1317
Vacuole 0.0052 0.0023 0.0021 0.0013 0.0047 0.002 0.0019 0.002 0.0009 0.001 0.0008 0.0006 0.0011 0.0026 0.0012 0.0018 0.0009 0.0011
Vacuole Periphery 0.002 0.0003 0.0004 0.0005 0.0012 0.0004 0.0009 0.0002 0.0003 0.0002 0.0011 0.0002 0.0001 0.0004 0.0002 0.0007 0.0002 0.0002

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 8.6372 6.1126 3.1034 3.0392 6.9991 8.2728 5.3672 2.9639 1.719 10.8485
Translational Efficiency – – – – – – – – – –

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✔
Translational Efficiency –

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
571 1525 1096 658 1504 1322 238 1427 2075 2847 1334 2085

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 602.00 694.41 767.69 757.16 651.32 678.93 843.69 772.27 637.75 687.22 781.25 767.50
Standard Deviation 69.62 101.52 94.09 121.03 77.16 100.57 96.61 114.81 78.32 101.37 98.92 117.02
Intensity Change Log 2 — 0.206024 0.350760 0.330835 — 0.059896 0.373346 0.245739 — 0.131934 0.362542 0.287239

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000032 0.000205 0.000371 0.003431 0.000057 0.000413 0.000693 0.000600 0.000050 0.000302 0.000428 0.001493
Bud Neck 0.002436 0.011228 0.006355 0.010426 0.011183 0.022523 0.001888 0.018355 0.008776 0.016473 0.005558 0.015853
Bud Site 0.000569 0.005850 0.010246 0.021842 0.001356 0.012995 0.002863 0.031840 0.001140 0.009168 0.008929 0.028685
Cell Periphery 0.000135 0.000124 0.000238 0.000290 0.000115 0.000181 0.000151 0.000271 0.000121 0.000150 0.000222 0.000277
Cytoplasm 0.565461* 0.394071* 0.155487* 0.322032* 0.393875* 0.334641* 0.109408* 0.281248* 0.441092* 0.366474* 0.147266* 0.294119*
Cytoplasmic Foci 0.094660 0.104850 0.006777 0.026116 0.131225 0.237072* 0.005408 0.021073 0.121163 0.166247 0.006533 0.022665
Eisosomes 0.000062 0.000060 0.000035 0.000082 0.000067 0.000115 0.000070 0.000052 0.000066 0.000086 0.000041 0.000061
Endoplasmic Reticulum 0.000231 0.001192 0.002550 0.002586 0.000986 0.000868 0.007394 0.003127 0.000779 0.001042 0.003414 0.002957
Endosome 0.002031 0.007125 0.001452 0.013992 0.004652 0.017580 0.002673 0.014504 0.003931 0.011980 0.001670 0.014342
Golgi 0.000262 0.001241 0.000179 0.003041 0.002004 0.005016 0.000125 0.003638 0.001525 0.002994 0.000169 0.003450
Lipid Particles 0.002710 0.006844 0.001190 0.001901 0.011474 0.017856 0.002073 0.002278 0.009062 0.011957 0.001347 0.002159
Mitochondria 0.000489 0.005839 0.002479 0.001716 0.005312 0.015792 0.000836 0.007733 0.003985 0.010461 0.002186 0.005834
Mitotic Spindle 0.000113 0.018509 0.010104 0.065698 0.005908 0.026169 0.013861 0.151823* 0.004313 0.022066 0.010774 0.124643*
None 0.025217 0.013817 0.011066 0.026611 0.010234 0.010409 0.009132 0.013546 0.014357 0.012234 0.010721 0.017669
Nuclear Periphery 0.000221 0.001345 0.001478 0.002216 0.001690 0.001202 0.001899 0.003254 0.001286 0.001279 0.001553 0.002926
Nuclear Periphery Foci 0.000797 0.002221 0.000866 0.003184 0.001377 0.002002 0.002447 0.003580 0.001217 0.002119 0.001148 0.003455
Nucleolus 0.003704 0.009325 0.006955 0.006392 0.010854 0.014168 0.005925 0.008381 0.008886 0.011574 0.006771 0.007753
Nucleus 0.291565* 0.388158* 0.769138* 0.424366* 0.390741* 0.232026* 0.803973* 0.359476* 0.363450* 0.315659* 0.775353* 0.379954*
Peroxisomes 0.000678 0.007059 0.000825 0.003428 0.006759 0.025998 0.000467 0.003270 0.005085 0.015853 0.000761 0.003320
Vacuole 0.008484 0.020002 0.011524 0.058550 0.009676 0.021399 0.028510 0.060585 0.009348 0.020651 0.014555 0.059943
Vacuole Periphery 0.000142 0.000934 0.000686 0.002101 0.000455 0.001574 0.000202 0.011366 0.000369 0.001232 0.000600 0.008442

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -9.20 -10.14 -2.83 -2.45 -1.75 -7.19 -7.47 -16.97 -7.37 3.23 -10.83 -12.26 -5.53 -4.15 -1.48
Bud Neck -7.14 -6.95 -6.38 -0.73 -0.19 -5.42 3.03 -5.78 0.58 -7.98 -6.64 -2.59 -7.71 -1.28 -4.73
Bud Site -6.79 -7.76 -7.89 -4.96 -2.22 -6.75 -4.57 -12.60 -6.81 -7.72 -9.15 -8.10 -14.80 -9.14 -6.02
Cell Periphery 1.09 -3.66 -3.51 -4.03 1.00 -3.17 -4.72 -5.44 -1.05 0.44 -2.71 -4.90 -6.52 -3.65 1.44
Cytoplasm 14.26 33.09 22.02 11.25 -7.67 6.28 24.97 18.83 11.47 -9.99 10.43 36.51 26.63 17.73 -10.40
Cytoplasmic Foci -1.66 15.36 13.15 20.03 -4.11 -11.36 26.48 22.69 32.99 -7.83 -7.00 29.91 26.10 36.69 -7.70
Eisosomes -0.96 0.24 -6.14 -5.76 -6.10 -8.21 -4.80 -4.70 3.17 2.87 -6.00 -1.52 -7.23 -2.21 -4.37
Endoplasmic Reticulum -9.13 -14.25 -14.11 -11.23 -3.96 0.70 -9.78 -14.32 -15.97 6.04 -2.70 -14.66 -19.41 -19.00 0.39
Endosome -5.50 -0.48 -3.75 -0.34 -3.88 -9.64 -0.58 -5.06 4.72 -2.61 -10.46 1.84 -6.19 2.61 -7.34
Golgi -6.16 -1.46 -2.51 0.21 -2.24 -3.49 3.28 0.39 3.88 -2.98 -3.27 3.22 -0.25 3.08 -3.71
Lipid Particles -6.34 3.72 2.04 8.22 -2.58 -3.26 8.06 8.94 10.24 0.15 -2.40 10.28 9.12 12.39 -4.16
Mitochondria -7.60 -4.00 -4.98 6.02 2.01 -5.30 7.44 1.53 5.88 -3.75 -6.07 3.17 1.34 6.64 -1.42
Mitotic Spindle -10.03 -9.09 -12.79 -9.48 -8.81 -7.81 -3.60 -23.53 -18.83 -12.20 -11.18 -8.30 -26.53 -21.43 -19.34
None 4.27 5.79 3.43 0.06 -1.52 -1.01 1.93 1.59 2.25 -0.76 1.36 4.12 3.33 2.29 -0.48
Nuclear Periphery -8.26 -19.41 -12.38 -8.24 -6.37 2.81 -5.66 -11.68 -12.84 -4.96 0.10 -8.29 -15.79 -15.32 -10.96
Nuclear Periphery Foci -4.14 -5.33 -5.44 -2.56 -0.62 -0.70 -3.35 -8.01 -7.91 0.33 -2.79 -5.46 -9.39 -7.66 -2.27
Nucleolus -7.08 -7.63 -2.71 3.09 4.50 0.09 0.81 -1.21 -1.36 -1.52 -1.98 -3.11 -1.78 0.03 1.67
Nucleus -5.64 -30.90 -6.94 -2.82 19.26 15.55 -16.01 3.69 -10.35 17.57 6.85 -34.44 -0.37 -6.54 32.02
Peroxisomes -10.35 -0.69 -2.71 7.29 -2.17 -10.09 7.23 3.46 13.08 -5.07 -10.51 6.65 2.91 14.21 -4.96
Vacuole -6.53 -4.09 -14.00 -12.27 -12.92 -8.18 -6.32 -18.07 -15.42 -4.12 -10.13 -7.37 -22.82 -19.56 -17.60
Vacuole Periphery -4.49 -3.08 -3.09 -0.34 -1.06 -4.76 3.43 -3.76 -2.64 -3.94 -5.85 -1.58 -4.12 -2.87 -3.71
Protein Complexes26S Proteasome complex37S mitochondrial small ribosomal subunit54S mitochondrial large ribosomal subunit60S cytosolic large ribosomal subunitAar2-U5 snRNPAnaphase-Promoting Complex variant 1Anaphase-Promoting Complex variant 2Anaphase-Promoting Complex variant 3Anaphase-Promoting Complex variant 4Arp2/3 complexATG1 kinase complexBLOC-1 complexCcr4-Not complexCentral kinetochore CTF19 complexCleavage and polyadenylation specificity factorcomplexCMG helicase complexCMG-Pol epsilon complexCOMPASS complexCOP9 signalosome COPI vesicle coat complexCoQ6 biosynthesis complexCore mediator complexCORVET complexCTF18-RFC complexCURI complex variant 1CURI complex variant 2CURI complex variant 3Cytoplasmic exosome complex, DIS3 variantDASH complexDNA-directed RNA Polymerase I complexDNA-directed RNA polymerase II complexDNA-directed RNA polymerase III complexDNA replication factor C complexEGO complexELG1-RFC complex Elongator holoenzyme complexEndoplasmic Reticulum Membrane ComplexEukaryotic translation initiation factor 2BcomplexEukaryotic translation initiation factor 3core complexExocystExomer complexFAR complexGeneral transcription factor complex TFIIDGeneral transcription factor complex TFIIHGET4-GET5 transmembrane domain recognition complexGID ubiquitin ligase complexGin4 complexGolgi transport complexGSE complexHMC complexHOPS complexINO80 chromatin remodeling complexLSM1-7 complexLsm1-7-Pat1 complexLSM2-7 complexLSM2-8 complexMCM complexMICOS complexMitochondrial electron transport complex IIIMitochondrial inner membrane pre-sequence translocasecomplexMitochondrial outer membrane translocase holocomplexMitochondrial proton-transporting ATP synthase complexMitochondrial pyruvate dehydrogenase complexNineTeen complexNOP8 60s ribosome pre-assembly complexNuA3 histone acetyltransferase complexNuA4 histone acetyltransferase complexNuclear condensin complexNuclear/nucleolar exosome complex, DIS3-RRP6 variantNuclear pore complexNucleolar ribonuclease MRP complexNucleolar ribonuclease P complexOligosaccharyl transferase complex variant 1Oligosaccharyl transferase complex variant 2PAF1 complexPAS complexPhosphatidylinositol 3-kinase complex IPolarisomePrefoldin complexPRP19-associated complexRad17 RFC-like complexRetromer complexRPD3L histone deacetylase complexRPD3S histone deacetylase complexRQC complexRSC complex variant RSC1RSC complex variant RSC2SAGA complexSCF-Met30 ubiquitin ligase complexSCF-Ufo1 ubiquitin ligase complexSEA complexSeptin complexSET3C histone deacetylase complexSignal recognition particleSLIK (SAGA-like) complexSmall ribosomal subunit processome, variant1Small ribosomal subunit processome, variant2Small ribosomal subunit processome, variant3SMC5-SMC6 SUMO ligase complexSm complexSpliceosomal commitment complexSPOTS complexSWI/SNF chromatin remodelling complexSwr1 chromatin remodelling complexTHO complexTranscription factor TFIIIC complexTranslocon complexTRAPPIII protein complexTRAPPII protein complexTRAPPI protein complexTREX complexU1 snRNPU2 snRNPU4 snRNPU4/U6 snRNPU4/U6.U5 tri-snRNP complexU5 snRNPU6 snRNP complexUTP-A complexUTP-B complexUTP-C complex variant 1UTP-C complex variant 2UTP-C complex variant 3Vacuolar proton translocating ATPase complex,Golgi variantVacuolar proton translocating ATPase complex,vacuole variantCellular Components••••••••••••••••actinbud neckcell peripherycytoplasmendosomeERGolgimitochondrionmixednuclear peripherynucleolusnucleusperoxisomespindle polevacuolar membranevacuole
Standard name
Human Ortholog
Description Protein kinase involved in the cell cycle checkpoint into anaphase; in complex with Mad1p and Bub3p, prevents progression into anaphase in presence of spindle damage; Cdc28p-mediated phosphorylation at Bub1p-T566 is important for degradation in anaphase and adaptation of checkpoint to prolonged mitotic arrest; associates with centromere DNA via Skp1p; involved in Sgo1p relocalization in response to sister kinetochore tension; paralog MAD3 arose from whole genome duplication
Localization
Cell Percentages cytoplasm (16%), nucleus (14%), mixed (38%)
Cell Cycle Regulation Yes
nucleus - S/G2 (1.2e-11)
Subcompartmental Group N/A

Bub1

Bub1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Bub1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available