Standard name
Human Ortholog
Description Glyceraldehyde-3-phosphate dehydrogenase (GAPDH), isozyme 3; involved in glycolysis and gluconeogenesis; tetramer that catalyzes the reaction of glyceraldehyde-3-phosphate to 1,3 bis-phosphoglycerate; detected in the cytoplasm and cell wall; GAPDH-derived antimicrobial peptides secreted by S. cerevisiae are active against a wide variety of wine-related yeasts and bacteria; binds AU-rich RNA
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 – – – – – –
Cell Periphery 0.15 0.42 0.52 0.48 0.49 0.48 0.34 0.15 0.52 0.31 0.54 0.36 – 0.44 0.43 0.2 0.26 0.16 0.66 0.67 0.68 0.64 0.59 0.66
Cytoplasm 0.95 0.67 0.74 0.68 0.63 0.67 0.75 0.9 0.74 0.84 0.78 0.84 – 0.69 0.67 0.75 0.86 0.82 0.3 0.25 0.27 0.3 0.35 0.27
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0.28 0.2 0.16 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 1 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 2 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 – – – – – –
Cell Periphery 14 20 101 124 145 272 117 37 327 158 343 171 – 17 18 13 17 8 124 178 522 82 127 207
Cytoplasm 87 32 146 174 185 379 257 216 472 434 494 395 – 27 28 48 57 42 56 67 203 38 74 85
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 18 13 8 0 1 1 0 0 1
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 1 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 4 0 0 0 10 0 2 – 1 0 0 0 0 4 8 4 2 5 7
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 1 6 0 2 1
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 1 1 0 0 0
Unique Cell Count 92 48 196 256 294 566 344 240 634 518 633 473 39 42 64 66 51 189 267 763 128 215 313
Labelled Cell Count 101 52 247 298 330 655 374 254 799 603 837 568 45 46 79 87 58 189 267 763 128 215 313


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 820.9 802.1 900.2 842.3 802.4 793.5 751.1 716.1 792.1 714.1 775.9 725.5 – 827.7 827.2 899.2 869.3 895.3 851.2 816.9 801.8
Std Deviation (1e-4) 146.5 148.3 156.3 146.1 170.4 165.8 168.1 166.4 160.8 175.8 152.0 176.5 – 218.5 151.2 128.7 118.3 126.4 153.0 183.7 173.3
Intensity Change (Log2) – – – -0.1 -0.17 -0.18 -0.26 -0.33 -0.18 -0.33 -0.21 -0.31 – -0.12 -0.12 -0.0 -0.05 -0.01 -0.08 -0.14 -0.17


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0
Cell Periphery -0.7 -0.5 -0.8 -4.0 -8.1 0 -5.2 0.7 -3.7 – -0.9 -1.0 -4.4 -3.6 -4.6
Cytoplasm -1.5 -2.7 -2.0 0.1 4.3 0 2.8 1.0 2.7 – -0.7 -1.0 0.1 2.0 1.2
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration – – – – – – – – – – – – – – – – – –
Actin 0.0041 0.0004 0.0095 0 0.0001 0.0042 0.0009 0.0003 0.0008 0 0.0047 0.0007 0.0116 0 0.0004 0.0001 0.001 0.0126
Bud 0.004 0.0004 0.0007 0 0.0005 0.0008 0.0015 0.0006 0.0003 0.0001 0.0005 0.0004 0.0034 0.0001 0.0006 0.0001 0.0006 0.0034
Bud Neck 0.0004 0 0.0006 0 0.0001 0.0002 0.0001 0.0001 0.0001 0 0.0004 0.0002 0.0005 0 0.0001 0 0.0001 0.0004
Bud Periphery 0.0013 0.0001 0.0008 0 0.0001 0.0016 0.0008 0 0.0002 0 0.0005 0.0001 0.0017 0 0.0002 0 0.0013 0.0015
Bud Site 0.0022 0.0002 0.0013 0 0.0006 0.0007 0.0008 0 0.0006 0 0.0015 0.0002 0.0065 0 0.0003 0.0002 0 0.001
Cell Periphery 0.0015 0.0003 0.0012 0 0.002 0.0002 0.0019 0.0001 0.0024 0 0.0051 0.0005 0.0033 0 0.0024 0.0002 0.0002 0.0003
Cytoplasm 0.0173 0.2877 0.1257 0.0019 0.0009 0.0161 0.0065 0.4388 0.1301 0.0102 0.0854 0.0089 0.0176 0.1315 0.2354 0.003 0.0028 0.1414
Cytoplasmic Foci 0.0105 0.0002 0.0313 0 0.2935 0.016 0.0014 0.0002 0.0167 0 0.0029 0.022 0.0836 0 0.0059 0.0034 0.0001 0.0003
Eisosomes 0.0015 0.0001 0.0016 0 0.01 0.0004 0.0032 0 0.0032 0 0.0227 0.0004 0.0034 0 0.0007 0.0013 0 0.0001
Endoplasmic Reticulum 0.0021 0 0.0095 0 0 0.0018 0.0001 0 0 0 0.0004 0.0001 0.0023 0 0 0 0.0016 0.0002
Endosome 0.0486 0 0.0591 0 0 0.007 0.0001 0 0.0001 0 0.0003 0.0002 0.0025 0 0.0002 0 0.0033 0.0002
Golgi 0.004 0 0.0125 0 0.0001 0.0013 0.0001 0 0.0002 0 0.0006 0.0001 0.0014 0 0.0001 0 0.0001 0.0001
Lipid Particles 0.0086 0 0.0069 0 0.0099 0.0021 0.0008 0 0.0023 0 0.0039 0.0014 0.0089 0 0.001 0.0003 0 0.0001
Mitochondria 0.0465 0.0121 0.0517 0.0017 0.374 0.0227 0.0714 0.0066 0.0497 0.0031 0.0652 0.0101 0.032 0.0038 0.022 0.0067 0.0053 0.0087
None 0.0183 0.0018 0.0311 0 0.0547 0.0042 0.0047 0.0014 0.0121 0.0001 0.0133 0.0167 0.0177 0.0001 0.0022 0.0057 0.0003 0.0016
Nuclear Periphery 0.0533 0.0172 0.0859 0.0013 0.0001 0.0188 0.0138 0.0151 0.0055 0.0024 0.0218 0.0661 0.0776 0.006 0.0158 0.0027 0.6508 0.0161
Nucleolus 0.2956 0.023 0.2255 0.028 0.2241 0.0716 0.3702 0.0048 0.1777 0.0052 0.3418 0.1551 0.2581 0.0061 0.2999 0.381 0.0015 0.046
Nucleus 0.4253 0.6543 0.2863 0.9668 0.0053 0.8026 0.5026 0.5291 0.5815 0.9782 0.3643 0.6961 0.4022 0.8518 0.3933 0.5796 0.0784 0.7589
Peroxisomes 0.0017 0.0001 0.0015 0 0.0157 0.0008 0.0006 0 0.0007 0 0.0014 0.0002 0.0023 0 0.0004 0.0001 0 0.0003
Punctate Nuclear 0.0417 0.0006 0.0381 0 0.0013 0.0202 0.008 0.0008 0.0045 0 0.0509 0.008 0.0428 0.0001 0.0006 0.0148 0.0002 0.0048
Vacuole 0.0055 0.0004 0.0092 0 0.0031 0.0027 0.0029 0.0005 0.0027 0.0001 0.0024 0.0016 0.01 0.0001 0.0046 0.0002 0.0281 0.0007
Vacuole Periphery 0.006 0.0011 0.0101 0.0001 0.0039 0.0041 0.0076 0.0016 0.0087 0.0004 0.01 0.011 0.0106 0.0004 0.0139 0.0006 0.2243 0.0014

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 17673.2478 18137.8682 19503.5782 16161.9002 18696.7683 18148.0748 19135.3418 18271.1041 21473.981 17382.6511
Translational Efficiency 0.8581 0.8727 0.8394 0.8638 0.8131 0.9127 1.0364 1.0042 0.915 1.1651

Hit Data

Dataset Hit
Protein Concentration –
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Glyceraldehyde-3-phosphate dehydrogenase (GAPDH), isozyme 3; involved in glycolysis and gluconeogenesis; tetramer that catalyzes the reaction of glyceraldehyde-3-phosphate to 1,3 bis-phosphoglycerate; detected in the cytoplasm and cell wall; GAPDH-derived antimicrobial peptides secreted by S. cerevisiae are active against a wide variety of wine-related yeasts and bacteria; binds AU-rich RNA
Localization
Cell Percentages cytoplasm (47%), cell periphery (1%), mixed (49%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Tdh3

Tdh3


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Tdh3-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available