Standard name
Human Ortholog
Description Cholinephosphate cytidylyltransferase; a rate-determining enzyme of the CDP-choline pathway for phosphatidylcholine synthesis, inhibited by Sec14p, activated upon lipid-binding; contains an element within the regulatory domain involved in both silencing and activation of enzymatic activity
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.13 0.15 0.06 0.09 0.07 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.07 0 0 0.05 0.06 0.12 0.22 0.12 0.16 0.17 0.24 0.18 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0.39 0.17 0.15 0.15 0.29 0.24 0.32 0.43 0.44 0.56 0.52 0.64 0.22 0.26 0.56 0.64 0.63 0.69 0 0.05 0 0.07 0.09 0.1
Nuclear Periphery 0.76 0.66 0.77 0.84 0.68 0.69 0.51 0.47 0.51 0.38 0.42 0.32 0.78 0.68 0.45 0.2 0.15 0.12 0.9 0.83 0.9 0.78 0.68 0.54
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.28 0.49 0.35 0.2 0.24 0.2 0.16 0.2 0.18 0.16 0.14 0.14 0.23 0.22 0.12 0.23 0.19 0.17 0 0.07 0 0.07 0.14 0.25
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 1 3 6 4 2 3 4 4 0 0 0 0 0 1 2 1 4 3 4 2
Bud Neck 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0
Bud Site 0 0 0 0 1 2 5 4 3 8 9 12 0 2 1 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 1 7 13 10 25 13 29 28 13 21 4 16 18 71 64 13 25 14 0 2 1 2 5 5
Endoplasmic Reticulum 0 0 1 0 1 2 1 0 0 0 1 0 1 0 1 8 8 10 0 0 1 0 0 3
Endosome 0 0 1 0 1 3 3 2 1 0 0 0 1 0 0 1 3 0 0 1 2 2 4 7
Golgi 0 0 1 0 0 0 0 0 0 0 0 0 0 1 0 1 0 0 0 0 1 1 1 2
Mitochondria 23 4 20 23 40 105 245 117 133 160 170 180 2 0 1 1 2 6 4 1 10 8 12 18
Nucleus 128 35 84 69 193 206 356 426 362 533 375 653 64 137 239 140 169 135 5 11 11 25 45 59
Nuclear Periphery 253 136 421 380 448 583 572 475 416 355 306 331 223 358 191 43 41 24 301 193 498 278 340 301
Nucleolus 3 1 6 10 13 18 35 34 20 22 28 31 2 4 4 4 13 6 3 1 3 4 12 15
Peroxisomes 0 0 0 0 0 0 0 1 0 0 0 0 0 2 1 0 0 1 0 0 0 0 0 0
SpindlePole 0 1 1 0 0 1 1 1 1 2 0 0 0 6 9 0 0 0 3 0 2 2 3 1
Vac/Vac Membrane 94 100 189 88 157 167 181 197 147 153 98 139 65 115 53 50 52 34 9 15 14 25 69 140
Unique Cell Count 331 206 546 450 659 844 1112 1001 819 946 722 1019 286 528 429 220 269 196 334 233 556 359 503 562
Labelled Cell Count 502 284 737 580 880 1103 1435 1290 1098 1257 995 1366 376 696 564 261 314 231 334 233 556 359 503 562


Nuclear Periphery

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 6.2 7.9 7.0 6.7 6.6 5.6 5.3 5.6 5.3 5.3 5.1 5.2 9.2 8.7 8.5 10.3 9.4 8.7 8.7 9.0 9.5
Std Deviation (1e-4) 1.1 2.1 1.3 1.5 1.7 1.4 1.3 1.4 1.3 1.2 1.2 1.2 2.0 2.1 2.0 2.5 2.5 2.6 1.6 1.6 1.5
Intensity Change (Log2) – – – -0.05 -0.08 -0.3 -0.38 -0.32 -0.39 -0.38 -0.46 -0.41 0.4 0.33 0.28 0.56 0.44 0.32 0.32 0.38 0.44


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm -0.2 1.4 -1.1 0.3 0.5 -1.1 -0.2 -2.8 -1.1 2.8 6.8 7.2 2.4 4.4 3.1
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 1.1 1.9 5.6 9.6 5.3 7.2 7.6 9.8 7.9 -2.5 -4.4 -3.7 -2.5 -2.4 -0.4
Nucleus 0 5.7 4.0 7.2 10.9 11.1 15.5 13.4 18.4 2.5 4.3 13.3 13.3 13.8 14.1
Nuclear Periphery 2.9 -3.5 -3.3 -10.0 -11.3 -9.8 -14.7 -12.4 -16.8 0.3 -3.4 -10.4 -14.7 -16.8 -15.9
Nucleolus 1.4 1.2 1.4 2.5 2.7 1.8 1.7 3.0 2.4 0 0 0 0 3.3 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole -5.3 -4.1 -6.2 -8.4 -6.5 -7.0 -8.2 -8.9 -9.7 -3.5 -4.7 -8.0 -3.2 -4.5 -4.5

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 11.5589 11.3035 11.1689 10.0896 10.3049 10.7621 8.7984 9.0559 8.2212 7.2612 7.4838 7.4015 9.3874 9.3233 8.2768 7.6379 6.7801 7.6105
Actin 0.0017 0 0.0004 0.0003 0.0001 0 0.009 0.0003 0.0143 0.1318 0.0001 0.0297 0.0109 0 0.005 0.0004 0.0068 0.0005
Bud 0.0001 0 0 0.0003 0 0 0.0006 0.0001 0.0002 0.0013 0 0.0005 0.0001 0 0.0001 0 0.0002 0
Bud Neck 0.0001 0 0 0.0001 0 0 0.0006 0.0003 0.0001 0.001 0 0.0001 0.0002 0 0.0007 0.0003 0.0003 0
Bud Periphery 0.0003 0 0.0002 0.0008 0 0 0.0007 0.0004 0.0003 0.0029 0.0001 0.0018 0.0002 0 0.0003 0 0.0003 0
Bud Site 0.0003 0 0 0.0008 0 0 0.0038 0.0001 0.0011 0.0032 0 0.0002 0.0012 0 0.0008 0 0.0009 0
Cell Periphery 0.0002 0 0.0001 0.0003 0 0 0.0002 0.0002 0.0001 0.0004 0 0 0 0 0.0001 0 0.0001 0
Cytoplasm 0.0005 0.0001 0.0001 0.0005 0 0 0.0032 0.0002 0.0046 0.0027 0 0.0003 0.0003 0 0.0004 0.0127 0.0065 0
Cytoplasmic Foci 0.0008 0 0.0002 0.0003 0 0 0.0023 0.0002 0.0018 0.0064 0 0.0002 0.0177 0 0.0008 0.0001 0.0086 0.0012
Eisosomes 0.0001 0 0 0 0 0 0.0002 0 0.0001 0.0005 0 0.0001 0.0001 0 0 0 0.0001 0
Endoplasmic Reticulum 0.0025 0.0007 0.0059 0.0023 0.0007 0.0003 0.0026 0.001 0.0056 0.009 0.0006 0.0018 0.0007 0.0008 0.0016 0.0017 0.0064 0.0006
Endosome 0.0027 0.0001 0.0022 0.0057 0.0001 0.0001 0.0121 0.0016 0.0098 0.0508 0.0001 0.002 0.0058 0 0.001 0.0004 0.0215 0.0008
Golgi 0.0018 0 0.0009 0.0004 0 0 0.0022 0.0003 0.0043 0.0144 0 0.0012 0.0034 0 0.0005 0 0.0076 0.0003
Lipid Particles 0.0124 0 0.0051 0.0011 0.0001 0 0.0027 0.0014 0.0025 0.01 0.0001 0.0006 0.0137 0 0.0019 0 0.0176 0.0022
Mitochondria 0.0076 0.0014 0.0031 0.0088 0.0004 0.0003 0.0058 0.0029 0.0066 0.241 0.0042 0.0054 0.0038 0.0004 0.0055 0.0007 0.007 0.0026
None 0.0003 0 0.0002 0.0002 0 0 0.003 0.0001 0.0004 0.0021 0.0001 0.0003 0.0005 0 0.0003 0.0108 0.004 0.0001
Nuclear Periphery 0.883 0.8723 0.89 0.8128 0.9639 0.97 0.7676 0.8912 0.8722 0.4386 0.9161 0.9139 0.8846 0.9165 0.9101 0.9092 0.8517 0.9693
Nucleolus 0.0026 0.0002 0.0001 0.0023 0.0002 0.0001 0.0034 0.0009 0.0002 0.0019 0.0001 0.0003 0.0002 0.0001 0.0011 0.0001 0.0009 0.0001
Nucleus 0.0375 0.0896 0.028 0.111 0.0054 0.0103 0.1329 0.032 0.0187 0.0151 0.002 0.0095 0.0095 0.0165 0.0075 0.0118 0.0157 0.0045
Peroxisomes 0.0003 0 0 0.0002 0 0 0.0009 0 0.0005 0.003 0 0.0005 0.0084 0 0.0002 0 0.0023 0.0003
Punctate Nuclear 0.0016 0.0001 0.0002 0.0002 0.0003 0.0001 0.0097 0.0002 0.0019 0.0059 0 0.001 0.0038 0 0.0003 0.0009 0.0077 0.0007
Vacuole 0.0026 0.0005 0.0026 0.0151 0.0002 0.0002 0.0023 0.0064 0.0016 0.0075 0.0003 0.0005 0.0004 0.0005 0.0015 0.0004 0.0018 0.0002
Vacuole Periphery 0.0411 0.035 0.0606 0.0364 0.0287 0.0186 0.0343 0.0602 0.0531 0.0506 0.076 0.0301 0.0346 0.065 0.0602 0.0503 0.0319 0.0166

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 25.097 23.9825 20.6664 20.2927 20.1969 18.1637 30.3108 19.0059 21.9087 21.9927
Translational Efficiency 0.9492 1.3444 1.151 1.3187 1.2906 1.5514 0.9996 1.8256 1.3396 1.2942

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
850 1384 2102 120 2109 14 196 13 2959 1398 2298 133

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 684.44 798.48 1008.62 1138.21 797.58 319.28 917.23 351.78 765.08 793.68 1000.83 1061.34
Standard Deviation 128.12 134.61 147.75 179.81 109.06 14.84 104.99 4.10 125.75 142.19 146.83 289.34
Intensity Change Log 2 — 0.222332 0.559387 0.733771 — -1.320807 0.201654 -1.180956 — -0.406954 0.377930 0.007738

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000246 0.000180 0.000272 0.000318 0.000099 0.000013 0.000276 0.000103 0.000141 0.000178 0.000272 0.000297
Bud Neck 0.000365 0.000669 0.001935 0.002178 0.000532 0.000002 0.000556 0.000002 0.000484 0.000662 0.001817 0.001965
Bud Site 0.000125 0.000715 0.000628 0.000610 0.000345 0.000006 0.000138 0.000009 0.000281 0.000708 0.000586 0.000551
Cell Periphery 0.000139 0.000104 0.000033 0.000026 0.000119 0.000031 0.000035 0.000175 0.000125 0.000103 0.000033 0.000040
Cytoplasm 0.022998 0.032638 0.003467 0.000690 0.016356 0.000013 0.002558 0.000015 0.018264 0.032312 0.003390 0.000624
Cytoplasmic Foci 0.004258 0.005205 0.000434 0.000189 0.002161 0.000006 0.000579 0.000010 0.002763 0.005153 0.000447 0.000171
Eisosomes 0.000021 0.000016 0.000003 0.000003 0.000006 0.000028 0.000016 0.001052 0.000010 0.000016 0.000004 0.000105
Endoplasmic Reticulum 0.021635 0.041932 0.010947 0.011951 0.040493 0.000000 0.015462 0.000000 0.035076 0.041512 0.011332 0.010783
Endosome 0.002709 0.004277 0.000931 0.001032 0.002600 0.000000 0.001010 0.000000 0.002631 0.004234 0.000938 0.000931
Golgi 0.000199 0.000856 0.000237 0.000188 0.000077 0.000003 0.000113 0.000001 0.000112 0.000847 0.000226 0.000170
Lipid Particles 0.013215 0.012855 0.000410 0.000204 0.005020 0.000011 0.001840 0.000010 0.007374 0.012726 0.000532 0.000185
Mitochondria 0.007255 0.005759 0.002946 0.002945 0.003920 0.000005 0.006221 0.000006 0.004878 0.005701 0.003226 0.002658
Mitotic Spindle 0.002053 0.002859 0.008801 0.029536 0.000365 0.000235 0.010312 0.000128 0.000850 0.002833 0.008930 0.026662
None 0.026218 0.011511 0.000919 0.000114 0.004851 0.999575* 0.001383 0.998319* 0.010989 0.021405 0.000958 0.097683
Nuclear Periphery 0.461928* 0.550961* 0.768932* 0.721621* 0.735410* 0.000000 0.665175* 0.000000 0.656850* 0.545444* 0.760082* 0.651087*
Nuclear Periphery Foci 0.153238 0.192789* 0.085366 0.172967 0.119345 0.000015 0.147829 0.000000 0.129081 0.190859* 0.090694 0.156060
Nucleolus 0.051576 0.016041 0.019683 0.011944 0.006862 0.000014 0.018449 0.000001 0.019706 0.015880 0.019578 0.010777
Nucleus 0.226415* 0.108750 0.087388 0.019856 0.048524 0.000006 0.122117 0.000003 0.099625 0.107661 0.090350 0.017915
Peroxisomes 0.000999 0.001142 0.000135 0.000271 0.000277 0.000037 0.000658 0.000166 0.000484 0.001131 0.000179 0.000260
Vacuole 0.002753 0.006545 0.003559 0.012508 0.007666 0.000000 0.004392 0.000000 0.006255 0.006479 0.003630 0.011286
Vacuole Periphery 0.001656 0.004196 0.002974 0.010850 0.004974 0.000000 0.000881 0.000000 0.004021 0.004154 0.002795 0.009789

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 1.56 -1.50 -0.98 -1.71 -0.40 NAN NAN NAN NAN NAN -1.84 -8.43 -2.35 -1.59 -0.05
Bud Neck -2.07 -7.75 -2.53 -2.12 -0.13 NAN NAN NAN NAN NAN -1.16 -7.24 -2.29 -2.03 0.05
Bud Site -1.68 -1.26 -1.89 0.15 -0.05 NAN NAN NAN NAN NAN -1.21 -0.88 -1.18 0.29 0.04
Cell Periphery 1.61 5.29 5.43 6.31 0.87 NAN NAN NAN NAN NAN 1.40 7.42 5.67 4.65 -0.55
Cytoplasm -2.31 7.23 8.33 11.14 5.56 NAN NAN NAN NAN NAN -4.42 10.33 12.58 11.18 6.07
Cytoplasmic Foci -1.02 4.82 5.44 5.58 0.64 NAN NAN NAN NAN NAN -2.55 4.71 6.10 5.61 0.84
Eisosomes 2.05 11.63 11.22 8.42 0.82 NAN NAN NAN NAN NAN -3.78 7.40 -1.81 -1.69 -1.92
Endoplasmic Reticulum -7.08 6.35 3.25 8.48 -0.31 NAN NAN NAN NAN NAN -2.24 14.43 8.79 9.15 0.32
Endosome -2.42 4.16 3.13 5.32 -0.48 NAN NAN NAN NAN NAN -2.98 6.97 4.60 5.61 -0.07
Golgi -2.58 -0.25 -0.16 2.38 0.12 NAN NAN NAN NAN NAN -2.92 -0.85 -0.95 2.47 0.20
Lipid Particles 0.01 9.58 9.93 8.92 0.73 NAN NAN NAN NAN NAN -3.67 10.96 12.60 8.93 1.41
Mitochondria 1.19 3.83 3.25 2.88 0.09 NAN NAN NAN NAN NAN -1.18 2.69 2.73 3.31 0.78
Mitotic Spindle -0.46 -4.64 -2.27 -2.23 -1.72 NAN NAN NAN NAN NAN -2.29 -6.21 -2.37 -2.21 -1.60
None 3.87 7.18 7.45 7.49 2.62 NAN NAN NAN NAN NAN -3.16 8.07 -3.35 -2.93 -3.75
Nuclear Periphery -5.66 -21.66 -10.10 -6.99 1.88 NAN NAN NAN NAN NAN 10.02 -11.03 0.30 -3.62 3.71
Nuclear Periphery Foci -4.24 8.94 -1.26 1.38 -6.19 NAN NAN NAN NAN NAN -8.75 9.15 -1.94 2.45 -4.80
Nucleolus 8.55 7.77 7.38 1.05 2.09 NAN NAN NAN NAN NAN 1.76 -0.27 2.45 1.41 2.58
Nucleus 9.30 11.51 17.36 11.37 10.99 NAN NAN NAN NAN NAN -1.21 0.81 13.46 11.95 12.51
Peroxisomes -0.74 8.76 6.37 3.46 -1.98 NAN NAN NAN NAN NAN -2.71 5.92 3.14 3.51 -1.24
Vacuole -4.41 -1.80 -2.25 -1.42 -2.00 NAN NAN NAN NAN NAN -0.27 2.52 -1.34 -1.27 -1.83
Vacuole Periphery -2.77 -3.03 -2.20 -1.58 -1.89 NAN NAN NAN NAN NAN -0.13 2.33 -1.53 -1.47 -1.85
Standard name
Human Ortholog
Description Cholinephosphate cytidylyltransferase; a rate-determining enzyme of the CDP-choline pathway for phosphatidylcholine synthesis, inhibited by Sec14p, activated upon lipid-binding; contains an element within the regulatory domain involved in both silencing and activation of enzymatic activity
Localization
Cell Percentages nuclear periphery (84%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Pct1

Pct1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Pct1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available