Standard name
Human Ortholog
Description Cytoplasmic trifunctional enzyme C1-tetrahydrofolate synthase; involved in single carbon metabolism and required for biosynthesis of purines, thymidylate, methionine, and histidine; null mutation causes auxotrophy for adenine and histidine
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 – 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 1.0 0.97 0.99 1.0 1.0 0.98 0.95 0.95 0.94 – 0.89 0.85 0.99 0.99 0.98 0.99 0.99 1.0 0.97 0.93 0.96 0.96 0.87 0.9
Endoplasmic Reticulum 0 0 0 0 0 0 0.06 0.05 0.05 – 0.07 0.07 0 0 0 0 0.06 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 1 3 – 10 9 0 0 0 0 0 0 0 0 0 0 1 1
Bud 0 0 1 1 2 0 0 0 0 – 2 2 1 1 0 0 0 0 0 0 0 0 4 3
Bud Neck 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 2 1 5 6 10 18 4 – 11 20 0 1 0 0 0 0 0 0 0 0 0 0
Cytoplasm 135 78 165 292 363 351 497 598 247 – 255 317 184 413 427 513 284 342 121 84 159 156 241 265
Endoplasmic Reticulum 0 2 1 1 1 4 29 29 13 – 19 26 2 0 6 16 17 15 0 1 2 0 0 6
Endosome 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 2
Golgi 0 0 0 0 0 0 0 0 0 – 1 2 0 1 1 2 1 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 1 – 2 2 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 – 1 0 0 0 0 0 0 0 0 0 1 1 14 2
Nuclear Periphery 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 1 0
Nucleolus 0 0 0 0 0 0 0 1 0 – 1 1 0 0 0 0 0 0 0 0 0 0 1 0
Peroxisomes 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 – 0 0 0 1 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 1 0 1 0 – 2 11 1 2 2 3 3 2 0 0 0 0 3 4
Unique Cell Count 135 80 166 293 364 357 525 632 263 288 371 186 418 435 516 287 342 125 90 166 163 276 295
Labelled Cell Count 135 80 169 295 371 362 536 648 268 304 390 188 419 436 534 305 359 125 90 166 163 276 295


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 162.2 133.4 88.2 86.2 90.1 77.1 72.8 66.7 57.5 – 49.7 46.7 155.1 153.6 129.9 133.2 162.4 160.0 123.2 109.9 124.3
Std Deviation (1e-4) 35.1 35.4 15.4 18.0 19.4 17.6 16.5 16.5 17.1 – 14.3 13.5 40.7 38.7 33.9 40.7 44.9 49.0 28.4 36.2 35.5
Intensity Change (Log2) – – – -0.03 0.03 -0.19 -0.28 -0.4 -0.62 – -0.83 -0.92 0.81 0.8 0.56 0.6 0.88 0.86 0.48 0.32 0.49


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 – 0 2.3 0 0 0 0 0 0
Cytoplasm 0.4 0.6 -1.0 -2.6 -2.7 -2.8 – -4.2 -4.9 -0.5 -0.6 -1.1 0 -0.5 1.4
Endoplasmic Reticulum 0 0 0 2.7 2.4 2.5 – 3.0 3.1 0 0 0 0 2.8 2.3
Endosome 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 138.6889 189.5873 160.3022 163.1751 140.9596 161.1963 160.5591 217.8931 207.3603 194.4177 175.998 196.2954 142.7447 180.1413 160.3822 153.3346 152.1619 161.5852
Actin 0.0022 0.0001 0.003 0.0001 0.0034 0.0239 0.0031 0.0002 0 0.0008 0.0027 0.0002 0.002 0.0002 0.0002 0.0001 0.0001 0
Bud 0.001 0.0001 0.001 0.0002 0.0011 0.0002 0.0014 0 0.0001 0 0.0001 0.0004 0.0005 0 0.0001 0.0001 0 0
Bud Neck 0.0002 0.0002 0.0003 0.0002 0.0033 0.0006 0.0001 0.0001 0.0001 0.0002 0.0002 0.0002 0.0002 0.0001 0.0001 0.0001 0.0002 0.0001
Bud Periphery 0.0006 0 0.0004 0 0.0006 0.0001 0.0008 0 0 0 0.0002 0.0001 0.0002 0 0 0 0 0
Bud Site 0.0005 0 0.0003 0 0.0017 0.0001 0.0005 0.0001 0 0.0003 0.0002 0 0.0001 0 0 0 0 0
Cell Periphery 0.0009 0.0001 0.0008 0 0.0007 0.0001 0.0004 0.0003 0 0 0.0001 0.0002 0.0001 0 0.0001 0 0 0
Cytoplasm 0.8051 0.9622 0.8772 0.915 0.8621 0.9111 0.8847 0.9857 0.9792 0.9237 0.8675 0.9258 0.9336 0.9906 0.9663 0.9702 0.9828 0.9942
Cytoplasmic Foci 0.0091 0 0.0131 0.0001 0.0038 0.0059 0.0038 0.0001 0.0005 0.0224 0.0029 0.0028 0.0011 0 0.0001 0.0001 0.0003 0.0001
Eisosomes 0.0003 0 0.0006 0 0.0003 0.0002 0.0003 0.0006 0 0 0 0 0.0001 0 0 0 0 0
Endoplasmic Reticulum 0.0012 0.0002 0.0006 0.0002 0.0004 0.0002 0.0023 0.0001 0.0001 0.0008 0.0042 0.0003 0.0004 0 0.0001 0 0.0001 0
Endosome 0.0023 0 0.0015 0.0001 0.002 0.002 0.0077 0 0 0.0045 0.0415 0.0002 0.0005 0 0 0 0.0001 0
Golgi 0.0004 0 0.0009 0 0.0023 0.0029 0.0008 0.0001 0 0.0015 0.0073 0 0.0001 0 0 0 0 0
Lipid Particles 0.0016 0 0.0035 0 0.0152 0.0088 0.0012 0.0001 0 0.0022 0.0024 0.0001 0.0001 0 0 0 0 0
Mitochondria 0.0103 0.0004 0.0106 0.0003 0.0135 0.0008 0.0048 0.002 0.0001 0.0018 0.0068 0.0022 0.001 0.0001 0.0003 0.0001 0.0001 0
None 0.0081 0.0001 0.0083 0.0002 0.0097 0.0015 0.0176 0.0003 0.0018 0.0001 0.0007 0.0071 0.003 0.0001 0.0003 0.0002 0.0005 0.0001
Nuclear Periphery 0.0178 0.0067 0.006 0.006 0.0071 0.0033 0.0183 0.0008 0.0011 0.026 0.0316 0.0044 0.0065 0.0021 0.0024 0.0025 0.0029 0.0006
Nucleolus 0.0239 0.0001 0.0136 0.0001 0.0051 0.0001 0.006 0.0004 0 0.0001 0.001 0.0119 0.0028 0 0.001 0 0 0
Nucleus 0.1 0.0286 0.0509 0.0742 0.0405 0.0114 0.0376 0.0086 0.0166 0.0102 0.017 0.0366 0.0456 0.0065 0.0282 0.0263 0.0123 0.0046
Peroxisomes 0.0006 0 0.0008 0 0.0166 0.024 0.0006 0 0 0.0037 0.0002 0 0.0001 0 0 0 0 0
Punctate Nuclear 0.0017 0 0.0007 0.0001 0.0006 0.0014 0.0009 0 0.0001 0.0003 0.0026 0.0006 0.0008 0.0002 0.0001 0.0001 0.0002 0
Vacuole 0.0077 0.0007 0.0029 0.002 0.0059 0.0008 0.0041 0.0002 0.0002 0.0002 0.0055 0.0029 0.0007 0 0.0005 0.0001 0.0002 0
Vacuole Periphery 0.0045 0.0005 0.003 0.0012 0.0041 0.0005 0.003 0.0003 0.0001 0.0012 0.0053 0.0039 0.0004 0 0.0003 0.0001 0.0001 0

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 269.3456 221.1621 176.2206 206.9332 206.9629 287.6347 249.7698 233.3789 256.6299 243.0745
Translational Efficiency 1.5094 1.3358 1.3465 1.304 1.341 1.4763 1.1755 1.2675 1.184 1.2016

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
974 1194 717 888 1204 1303 808 146 2178 2497 1525 1034

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 5209.40 8037.77 7032.15 5694.93 6774.19 8161.98 5494.71 6424.63 6074.42 8102.59 6217.56 5797.96
Standard Deviation 1186.81 1988.06 1269.62 1421.10 1670.11 1964.90 1085.45 1525.64 1666.47 1976.99 1403.90 1458.63
Intensity Change Log 2 — 0.625678 0.432849 0.128561 — 0.268871 -0.302005 -0.076435 — 0.434911 0.063965 0.016277

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000036 0.000051 0.000086 0.001188 0.000060 0.000039 0.000103 0.000338 0.000049 0.000045 0.000095 0.001068
Bud Neck 0.000158 0.000225 0.001161 0.006568 0.000286 0.000138 0.001633 0.004537 0.000228 0.000179 0.001411 0.006281
Bud Site 0.000486 0.000439 0.000943 0.017744 0.001306 0.000283 0.000859 0.011315 0.000939 0.000357 0.000899 0.016836
Cell Periphery 0.002548 0.000820 0.002302 0.002990 0.003023 0.000615 0.000338 0.014298 0.002811 0.000713 0.001261 0.004587
Cytoplasm 0.875277* 0.779824* 0.667398* 0.728806* 0.843924* 0.821008* 0.469035* 0.706723* 0.857945* 0.801315* 0.562298* 0.725688*
Cytoplasmic Foci 0.002246 0.003805 0.011240 0.012854 0.004031 0.002807 0.003339 0.021013 0.003233 0.003284 0.007054 0.014006
Eisosomes 0.000001 0.000001 0.000002 0.000023 0.000001 0.000001 0.000001 0.000007 0.000001 0.000001 0.000002 0.000021
Endoplasmic Reticulum 0.000017 0.000012 0.000018 0.000278 0.000067 0.000037 0.000055 0.000014 0.000045 0.000025 0.000037 0.000240
Endosome 0.000016 0.000018 0.000047 0.005913 0.000045 0.000129 0.000099 0.000341 0.000032 0.000076 0.000074 0.005126
Golgi 0.002218 0.000767 0.000871 0.006885 0.003568 0.000440 0.000377 0.000641 0.002965 0.000596 0.000609 0.006004
Lipid Particles 0.000959 0.000531 0.001548 0.002979 0.001134 0.000281 0.000531 0.016128 0.001056 0.000400 0.001009 0.004836
Mitochondria 0.000081 0.000130 0.000168 0.005517 0.000149 0.000090 0.000352 0.000119 0.000119 0.000109 0.000266 0.004755
Mitotic Spindle 0.000069 0.000154 0.000397 0.009694 0.000250 0.000033 0.000749 0.006281 0.000169 0.000090 0.000584 0.009212
None 0.013358 0.012509 0.008535 0.007460 0.011964 0.022170 0.007773 0.004162 0.012587 0.017550 0.008131 0.006994
Nuclear Periphery 0.000093 0.000090 0.000376 0.000466 0.000149 0.000067 0.000757 0.000359 0.000124 0.000078 0.000578 0.000451
Nuclear Periphery Foci 0.001632 0.000681 0.001210 0.002308 0.001318 0.000558 0.001366 0.003311 0.001459 0.000617 0.001293 0.002449
Nucleolus 0.000040 0.000091 0.000071 0.000490 0.000048 0.000195 0.000158 0.000092 0.000044 0.000146 0.000117 0.000434
Nucleus 0.089932 0.194615* 0.290890* 0.120145 0.115604 0.148317* 0.498847* 0.078523 0.104123 0.170455* 0.401073* 0.114268
Peroxisomes 0.000003 0.000007 0.000005 0.000844 0.000007 0.000023 0.000008 0.000142 0.000005 0.000016 0.000006 0.000745
Vacuole 0.007213 0.004327 0.012504 0.062144 0.010025 0.002632 0.013455 0.131292 0.008768 0.003443 0.013008 0.071908
Vacuole Periphery 0.003616 0.000903 0.000228 0.004704 0.003041 0.000137 0.000167 0.000363 0.003298 0.000503 0.000195 0.004091

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -1.72 -5.46 -2.12 -1.96 -1.45 1.66 -6.11 -4.12 -4.49 -2.58 0.03 -8.05 -2.31 -2.31 -1.57
Bud Neck -3.74 -8.73 -8.95 -8.66 -4.30 3.05 -12.70 -4.34 -4.50 0.06 0.10 -15.28 -9.64 -9.65 -2.96
Bud Site 0.02 -4.79 -5.92 -5.92 -5.20 2.03 -1.48 -4.01 -4.62 -3.70 1.98 -2.80 -6.31 -6.86 -5.72
Cell Periphery 2.77 -0.51 1.44 -1.18 2.63 5.41 5.16 -2.76 -4.09 -4.03 5.67 2.77 0.65 -3.35 -1.30
Cytoplasm 15.45 27.44 22.94 10.30 -4.97 3.42 47.39 9.54 8.59 -9.81 12.94 49.49 23.62 15.94 -16.20
Cytoplasmic Foci -5.06 -10.74 -9.47 -7.08 2.81 1.90 -6.05 -6.79 -7.03 -4.78 -1.84 -11.96 -10.74 -10.15 -0.72
Eisosomes -0.76 -6.80 -2.55 -2.50 -1.48 2.13 -9.09 -7.36 -7.83 -3.86 1.42 -11.05 -2.88 -2.96 -1.62
Endoplasmic Reticulum 0.83 -2.55 -2.83 -2.96 -2.41 0.73 -0.37 1.37 0.60 9.01 0.83 -0.59 -1.38 -2.21 -1.27
Endosome -0.98 -5.81 -3.31 -3.30 -3.19 -0.68 -4.58 -1.60 -0.62 -0.31 -0.71 -6.11 -3.34 -3.22 -3.11
Golgi 2.18 1.88 -0.12 -1.51 -1.31 4.42 4.41 3.97 -1.60 -1.56 4.80 4.62 0.82 -1.68 -1.57
Lipid Particles 1.12 -4.64 -7.19 -8.21 -3.18 4.77 -1.68 -5.19 -5.49 -5.03 3.94 -4.68 -8.57 -9.74 -6.62
Mitochondria -1.90 -7.26 -1.54 -1.49 -1.39 2.76 -10.84 1.64 0.28 8.78 0.62 -12.64 -1.49 -1.51 -1.21
Mitotic Spindle -1.35 -3.60 -3.36 -3.27 -2.88 1.35 -3.33 -1.14 -1.22 -0.39 0.76 -4.03 -3.47 -3.54 -2.23
None 1.04 5.07 7.48 7.33 4.70 -5.30 4.65 7.29 10.33 4.56 -3.87 6.84 9.57 11.87 5.36
Nuclear Periphery -0.94 -10.00 -9.72 -9.75 3.09 5.24 -6.48 -5.17 -6.34 3.90 3.37 -9.13 -10.24 -11.64 5.04
Nuclear Periphery Foci 2.17 -0.34 -1.86 -7.43 -2.26 4.31 -0.89 -4.24 -5.45 -3.33 3.95 -0.91 -4.50 -10.04 -3.18
Nucleolus -1.32 -9.41 -3.95 -2.81 -2.99 -1.04 -15.19 -2.69 0.78 10.15 -1.34 -17.08 -3.97 -1.46 -1.50
Nucleus -18.34 -26.90 -9.78 7.40 17.97 -5.94 -43.01 0.89 3.60 28.32 -16.87 -46.71 -7.02 5.71 35.28
Peroxisomes -1.84 -4.57 -1.44 -1.41 -1.41 -0.78 -3.63 -2.00 -1.35 -1.63 -0.99 -4.79 -1.49 -1.42 -1.43
Vacuole 1.57 -6.88 -18.74 -19.31 -14.28 5.07 -10.26 -9.60 -10.11 -7.20 4.96 -12.22 -20.77 -21.92 -14.90
Vacuole Periphery 1.73 2.15 1.22 -1.13 -2.47 3.72 3.63 3.49 -2.60 -1.71 3.36 3.74 1.99 -1.90 -2.57
Standard name
Human Ortholog
Description Cytoplasmic trifunctional enzyme C1-tetrahydrofolate synthase; involved in single carbon metabolism and required for biosynthesis of purines, thymidylate, methionine, and histidine; null mutation causes auxotrophy for adenine and histidine
Localization
Cell Percentages cytoplasm (100%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-2

Ade3

Ade3


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ade3-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available