Standard name | |
---|---|
Human Ortholog | |
Description | ESCRT-I subunit required to stabilize ESCRT-I core complex oligomers; the ESCRT-I core complex (Stp22p, Vps28p, Srn2p) is involved in ubiquitin-dependent sorting of proteins into the endosome; deletion mutant is sensitive to rapamycin and nystatin |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 3 | 11 | 2 | 6 | 5 | 1 | – | 2 | 2 | 4 | 2 | 3 | 6 | 5 | 3 | 2 | 1 | 0 | 6 | 6 | 3 | 0 | 2 | 2 |
Bud | 3 | 6 | 5 | 25 | 40 | 19 | – | 49 | 34 | 62 | 45 | 50 | 4 | 6 | 4 | 1 | 1 | 0 | 1 | 2 | 1 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 4 | 0 | 1 | 0 | – | 1 | 0 | 3 | 0 | 1 | 0 | 0 | 1 | 0 | 1 | 0 | 2 | 1 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | – | 2 | 1 | 7 | 6 | 7 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 1 | 0 | 0 | 0 | 0 | 0 | – | 1 | 1 | 4 | 2 | 2 | 1 | 1 | 0 | 1 | 2 | 2 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 274 | 132 | 86 | 59 | 133 | 144 | – | 292 | 193 | 243 | 169 | 200 | 131 | 117 | 171 | 45 | 59 | 23 | 173 | 64 | 67 | 29 | 27 | 45 |
Endoplasmic Reticulum | 13 | 15 | 5 | 8 | 7 | 8 | – | 5 | 10 | 5 | 5 | 7 | 16 | 11 | 16 | 2 | 3 | 1 | 4 | 0 | 0 | 0 | 1 | 0 |
Endosome | 44 | 39 | 0 | 106 | 297 | 42 | – | 83 | 42 | 68 | 28 | 30 | 21 | 82 | 98 | 6 | 10 | 4 | 154 | 121 | 50 | 31 | 187 | 156 |
Golgi | 0 | 0 | 0 | 0 | 1 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 3 | 1 | 0 | 2 | 1 | 0 | 0 | 0 | 1 |
Mitochondria | 10 | 0 | 13 | 110 | 40 | 173 | – | 328 | 324 | 320 | 286 | 343 | 3 | 0 | 1 | 0 | 3 | 0 | 12 | 2 | 8 | 4 | 10 | 7 |
Nucleus | 1 | 0 | 0 | 0 | 1 | 3 | – | 4 | 3 | 1 | 1 | 2 | 0 | 2 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 1 | – | 2 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 1 | 0 | 0 | 5 | 4 | 3 | – | 3 | 3 | 4 | 1 | 3 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 17 | 40 | 6 | – | 16 | 3 | 22 | 4 | 15 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 |
SpindlePole | 2 | 6 | 0 | 44 | 105 | 24 | – | 84 | 29 | 78 | 45 | 56 | 1 | 8 | 6 | 2 | 6 | 2 | 8 | 6 | 5 | 2 | 8 | 9 |
Vac/Vac Membrane | 17 | 6 | 2 | 3 | 23 | 12 | – | 25 | 11 | 21 | 12 | 11 | 7 | 7 | 11 | 3 | 6 | 0 | 10 | 6 | 8 | 4 | 18 | 19 |
Unique Cell Count | 348 | 190 | 108 | 298 | 537 | 375 | 720 | 521 | 647 | 462 | 573 | 170 | 214 | 286 | 59 | 81 | 28 | 385 | 219 | 154 | 75 | 264 | 249 | |
Labelled Cell Count | 369 | 215 | 117 | 383 | 697 | 436 | 897 | 657 | 842 | 606 | 730 | 190 | 240 | 318 | 65 | 93 | 32 | 385 | 219 | 154 | 75 | 264 | 249 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 6.0 | 6.8 | 4.0 | 4.5 | 5.1 | 3.9 | – | 4.2 | 3.9 | 4.1 | 3.8 | 3.8 | 6.2 | 6.5 | 6.4 | 8.1 | 8.2 | 8.0 | 5.8 | 6.5 | 7.0 |
Std Deviation (1e-4) | 0.8 | 1.0 | 0.7 | 1.0 | 1.2 | 0.9 | – | 1.1 | 0.9 | 0.9 | 0.7 | 0.8 | 0.9 | 1.0 | 0.9 | 1.3 | 1.3 | 1.8 | 0.9 | 0.8 | 1.7 |
Intensity Change (Log2) | – | – | – | 0.19 | 0.34 | -0.03 | – | 0.06 | -0.04 | 0.06 | -0.06 | -0.06 | 0.64 | 0.71 | 0.68 | 1.03 | 1.04 | 1.01 | 0.54 | 0.7 | 0.81 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 1.0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | -11.1 | -11.0 | -7.6 | – | -7.6 | -8.1 | -8.2 | -8.1 | -8.6 | -0.5 | -4.4 | -3.7 | -0.5 | -1.1 | 0.3 |
Endoplasmic Reticulum | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 1.5 | 0.2 | 0.4 | 0 | 0 | 0 |
Endosome | 0 | 10.5 | 0 | – | 0 | 0 | 0 | 0 | 0 | 3.8 | 7.5 | 7.0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | -1.6 | 0 | – | 0 | 0 | 0 | 0 | 0 | -3.6 | 0 | 0 | 0 | -2.0 | 0 |
Nucleus | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 2.9 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 5.0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 1.1487 | 1.6424 | 1.1803 | 1.3014 | 1.0736 | 1.3663 | 0.5508 | 0.9274 | 0.8636 | 0.7656 | 0.4084 | 0.8153 | 2.4355 | 2.8293 | 2.3372 | 3.7346 | 2.5305 | 2.8394 |
Actin | 0.0243 | 0.0018 | 0.0107 | 0.046 | 0.0001 | 0.0022 | 0.0236 | 0.0108 | 0.0064 | 0.0001 | 0.0123 | 0.0034 | 0.02 | 0.0002 | 0.0004 | 0.0453 | 0.0163 | 0.0101 |
Bud | 0.0002 | 0.0001 | 0.0002 | 0.0002 | 0.0001 | 0.0002 | 0.001 | 0.004 | 0.0003 | 0.0002 | 0.0215 | 0.0002 | 0.0004 | 0.0001 | 0.0002 | 0.0016 | 0.0002 | 0.0001 |
Bud Neck | 0.0031 | 0.0003 | 0.0004 | 0.0042 | 0.0003 | 0.0043 | 0.0004 | 0.0004 | 0.0026 | 0.0003 | 0.0033 | 0.0007 | 0.0005 | 0.0002 | 0.0002 | 0.0003 | 0.0005 | 0.0003 |
Bud Periphery | 0.0003 | 0.0001 | 0.0002 | 0.0002 | 0 | 0.0002 | 0.0015 | 0.0025 | 0.0002 | 0.0001 | 0.0208 | 0.0002 | 0.0006 | 0.0001 | 0.0001 | 0.0019 | 0.0003 | 0.0001 |
Bud Site | 0.0012 | 0.0013 | 0.0007 | 0.0007 | 0 | 0.0015 | 0.0026 | 0.0088 | 0.0009 | 0.0004 | 0.018 | 0.0003 | 0.0015 | 0.0009 | 0.0003 | 0.0099 | 0.0004 | 0.0003 |
Cell Periphery | 0.0002 | 0.0001 | 0.0001 | 0.0001 | 0 | 0.0001 | 0.0003 | 0.0002 | 0.0001 | 0 | 0.0004 | 0 | 0.0001 | 0.0003 | 0.0001 | 0.0001 | 0.0001 | 0 |
Cytoplasm | 0.3723 | 0.422 | 0.5121 | 0.362 | 0.6067 | 0.4025 | 0.3138 | 0.4975 | 0.4226 | 0.5353 | 0.3581 | 0.4164 | 0.2525 | 0.4915 | 0.477 | 0.3241 | 0.2535 | 0.3979 |
Cytoplasmic Foci | 0.1884 | 0.1927 | 0.1334 | 0.2085 | 0.0946 | 0.22 | 0.1934 | 0.1526 | 0.1369 | 0.0789 | 0.2201 | 0.2353 | 0.1657 | 0.1276 | 0.0952 | 0.1114 | 0.1429 | 0.2294 |
Eisosomes | 0.0002 | 0 | 0.0003 | 0.0005 | 0 | 0 | 0.0006 | 0 | 0.0001 | 0 | 0 | 0 | 0.0002 | 0 | 0 | 0.0002 | 0.0001 | 0 |
Endoplasmic Reticulum | 0.0111 | 0.0066 | 0.0068 | 0.0088 | 0.0056 | 0.007 | 0.0107 | 0.0054 | 0.0076 | 0.0036 | 0.0098 | 0.0038 | 0.0073 | 0.0077 | 0.0071 | 0.0145 | 0.0019 | 0.0081 |
Endosome | 0.2298 | 0.2032 | 0.1835 | 0.2296 | 0.1694 | 0.2113 | 0.2199 | 0.1623 | 0.2068 | 0.1954 | 0.2096 | 0.2436 | 0.1553 | 0.154 | 0.1544 | 0.1584 | 0.2255 | 0.193 |
Golgi | 0.014 | 0.0067 | 0.0067 | 0.0089 | 0.0025 | 0.0073 | 0.0181 | 0.0086 | 0.0234 | 0.0195 | 0.0415 | 0.0144 | 0.0185 | 0.006 | 0.0047 | 0.0052 | 0.0152 | 0.0068 |
Lipid Particles | 0.0137 | 0.0055 | 0.0027 | 0.0055 | 0.0004 | 0.0077 | 0.0132 | 0.0015 | 0.0076 | 0.004 | 0.0086 | 0.0036 | 0.0253 | 0.0046 | 0.0033 | 0.0036 | 0.0211 | 0.0076 |
Mitochondria | 0.0025 | 0.0006 | 0.0016 | 0.0013 | 0.0008 | 0.0014 | 0.0183 | 0.0009 | 0.014 | 0.0013 | 0.0112 | 0.0018 | 0.0048 | 0.0006 | 0.0016 | 0.005 | 0.0054 | 0.0009 |
None | 0.0972 | 0.1381 | 0.12 | 0.0916 | 0.1107 | 0.1069 | 0.1431 | 0.1262 | 0.1299 | 0.1431 | 0.0087 | 0.0536 | 0.2705 | 0.1897 | 0.2376 | 0.2929 | 0.2189 | 0.1193 |
Nuclear Periphery | 0.0078 | 0.0033 | 0.0046 | 0.004 | 0.0014 | 0.0042 | 0.0113 | 0.0021 | 0.0104 | 0.0013 | 0.0037 | 0.001 | 0.0057 | 0.0018 | 0.0017 | 0.0021 | 0.0078 | 0.0063 |
Nucleolus | 0.0012 | 0.0002 | 0.0001 | 0.0001 | 0.0001 | 0.0003 | 0.0006 | 0.0001 | 0.0004 | 0.0001 | 0.0004 | 0.0001 | 0.0003 | 0.0002 | 0.0002 | 0.0001 | 0.0024 | 0.0001 |
Nucleus | 0.0042 | 0.0021 | 0.002 | 0.0015 | 0.0015 | 0.0019 | 0.0049 | 0.0015 | 0.0091 | 0.0008 | 0.0024 | 0.0012 | 0.0012 | 0.0015 | 0.0008 | 0.0009 | 0.0179 | 0.0017 |
Peroxisomes | 0.0083 | 0.0026 | 0.0035 | 0.0129 | 0.0004 | 0.0076 | 0.0062 | 0.0034 | 0.0094 | 0.0035 | 0.0184 | 0.0135 | 0.0548 | 0.0014 | 0.0013 | 0.0131 | 0.0554 | 0.0028 |
Punctate Nuclear | 0.0115 | 0.0027 | 0.0024 | 0.002 | 0.0007 | 0.0067 | 0.0056 | 0.0021 | 0.0018 | 0.0005 | 0.0021 | 0.0017 | 0.0099 | 0.0013 | 0.0006 | 0.001 | 0.0086 | 0.005 |
Vacuole | 0.0068 | 0.0086 | 0.0066 | 0.009 | 0.0035 | 0.0055 | 0.0089 | 0.0079 | 0.0071 | 0.0089 | 0.0209 | 0.0042 | 0.0038 | 0.0093 | 0.0102 | 0.0061 | 0.0041 | 0.0078 |
Vacuole Periphery | 0.0017 | 0.0013 | 0.0014 | 0.0022 | 0.0012 | 0.0012 | 0.0019 | 0.0011 | 0.0022 | 0.0027 | 0.0081 | 0.0011 | 0.001 | 0.001 | 0.0029 | 0.0023 | 0.0014 | 0.0022 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 10.8177 | 12.8938 | 19.1355 | 21.7052 | 16.3632 | 7.2078 | 9.7777 | 14.2984 | 16.6497 | 15.72 |
Translational Efficiency | 1.2417 | 0.7503 | 0.8343 | 0.4824 | 0.7864 | 1.7493 | 0.9467 | 0.9431 | 1.0788 | 1.3259 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✔ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
145 | 918 | 130 | 243 | 1659 | 1427 | 1197 | 1176 | 1804 | 2345 | 1327 | 1419 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 654.39 | 804.15 | 1045.28 | 959.82 | 753.64 | 801.30 | 933.34 | 891.53 | 745.66 | 802.42 | 944.31 | 903.22 |
Standard Deviation | 80.28 | 122.19 | 128.50 | 125.81 | 100.38 | 107.51 | 107.04 | 123.39 | 102.53 | 113.50 | 114.28 | 126.45 |
Intensity Change Log 2 | — | 0.297314 | 0.675667 | 0.552613 | — | 0.088467 | 0.308527 | 0.242408 | — | 0.189300 | 0.490816 | 0.394900 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000842 | 0.000983 | 0.005343 | 0.005565 | 0.000743 | 0.000557 | 0.000922 | 0.002991 | 0.000751 | 0.000723 | 0.001355 | 0.003432 |
Bud Neck | 0.028615 | 0.012712 | 0.017067 | 0.012395 | 0.021085 | 0.016641 | 0.013501 | 0.009864 | 0.021691 | 0.015103 | 0.013851 | 0.010297 |
Bud Site | 0.010009 | 0.013596 | 0.023468 | 0.048348 | 0.006523 | 0.008359 | 0.021955 | 0.032618 | 0.006803 | 0.010409 | 0.022103 | 0.035312 |
Cell Periphery | 0.000092 | 0.000055 | 0.000233 | 0.000101 | 0.000090 | 0.000076 | 0.000054 | 0.000067 | 0.000090 | 0.000068 | 0.000072 | 0.000073 |
Cytoplasm | 0.087559 | 0.157387* | 0.115784* | 0.142524* | 0.109984* | 0.129868* | 0.129384* | 0.208318* | 0.108181* | 0.140641* | 0.128052* | 0.197051* |
Cytoplasmic Foci | 0.452600* | 0.369676* | 0.132566 | 0.193509* | 0.356479* | 0.388501* | 0.218655* | 0.218283* | 0.364205* | 0.381132* | 0.210221* | 0.214040* |
Eisosomes | 0.000284 | 0.000078 | 0.000135 | 0.000068 | 0.000251 | 0.000135 | 0.000039 | 0.000056 | 0.000254 | 0.000113 | 0.000048 | 0.000058 |
Endoplasmic Reticulum | 0.000326 | 0.001264 | 0.010588 | 0.002655 | 0.001455 | 0.000883 | 0.001789 | 0.001080 | 0.001365 | 0.001032 | 0.002651 | 0.001350 |
Endosome | 0.195341 | 0.219329* | 0.152114 | 0.128636 | 0.283532* | 0.240441* | 0.244030* | 0.135009 | 0.276443* | 0.232176* | 0.235026* | 0.133918 |
Golgi | 0.032914 | 0.018219 | 0.001780 | 0.002812 | 0.035838 | 0.020662 | 0.004493 | 0.006044 | 0.035603 | 0.019706 | 0.004227 | 0.005490 |
Lipid Particles | 0.029992 | 0.019835 | 0.023856 | 0.012693 | 0.045622 | 0.029661 | 0.012600 | 0.006217 | 0.044366 | 0.025814 | 0.013703 | 0.007326 |
Mitochondria | 0.015229 | 0.005462 | 0.000694 | 0.000627 | 0.009358 | 0.009963 | 0.002469 | 0.001787 | 0.009830 | 0.008201 | 0.002295 | 0.001589 |
Mitotic Spindle | 0.001684 | 0.013101 | 0.043864 | 0.059508 | 0.003762 | 0.006175 | 0.065367 | 0.043374 | 0.003595 | 0.008887 | 0.063260 | 0.046137 |
None | 0.000167 | 0.000352 | 0.000493 | 0.000450 | 0.000628 | 0.000422 | 0.000383 | 0.001249 | 0.000591 | 0.000395 | 0.000394 | 0.001112 |
Nuclear Periphery | 0.000124 | 0.000388 | 0.001813 | 0.001156 | 0.000229 | 0.000276 | 0.001759 | 0.000772 | 0.000221 | 0.000320 | 0.001764 | 0.000838 |
Nuclear Periphery Foci | 0.001303 | 0.001574 | 0.019961 | 0.011924 | 0.000771 | 0.001026 | 0.010456 | 0.003645 | 0.000814 | 0.001241 | 0.011387 | 0.005063 |
Nucleolus | 0.000924 | 0.001487 | 0.004707 | 0.001346 | 0.000577 | 0.001077 | 0.001905 | 0.000840 | 0.000605 | 0.001237 | 0.002179 | 0.000927 |
Nucleus | 0.014603 | 0.010098 | 0.121776 | 0.017274 | 0.009621 | 0.009551 | 0.014812 | 0.016649 | 0.010021 | 0.009765 | 0.025291 | 0.016756 |
Peroxisomes | 0.085068 | 0.024618 | 0.039639 | 0.052584 | 0.031656 | 0.029132 | 0.034635 | 0.053689 | 0.035949 | 0.027365 | 0.035125 | 0.053500 |
Vacuole | 0.040846 | 0.125261 | 0.282567* | 0.305004* | 0.078264 | 0.102446 | 0.218830* | 0.254268* | 0.075256 | 0.111377 | 0.225074* | 0.262956* |
Vacuole Periphery | 0.001480 | 0.004527 | 0.001550 | 0.000823 | 0.003531 | 0.004149 | 0.001963 | 0.003180 | 0.003366 | 0.004297 | 0.001922 | 0.002776 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -0.34 | -2.11 | -2.21 | -2.11 | 0.55 | 1.43 | -0.69 | -2.88 | -3.38 | -2.64 | 0.17 | -2.01 | -3.59 | -3.65 | -2.38 |
Bud Neck | 3.03 | 1.69 | 3.38 | 1.20 | 2.27 | 2.22 | 2.23 | 5.69 | 4.38 | 4.49 | 3.72 | 2.57 | 6.40 | 4.24 | 5.00 |
Bud Site | -1.29 | -3.72 | -4.48 | -4.25 | -1.50 | -1.98 | -8.31 | -7.82 | -6.90 | -1.62 | -4.41 | -9.08 | -9.26 | -7.49 | -2.27 |
Cell Periphery | 2.69 | -5.34 | -0.15 | -4.15 | 5.55 | 1.49 | 4.41 | 4.40 | 2.53 | -0.19 | 2.90 | 1.86 | 3.79 | 0.87 | 2.21 |
Cytoplasm | -5.24 | -2.20 | -4.23 | -0.21 | -1.36 | -3.00 | -5.32 | -12.68 | -10.06 | -7.20 | -5.56 | -5.65 | -13.01 | -8.79 | -6.98 |
Cytoplasmic Foci | 4.30 | 15.07 | 14.88 | 18.46 | -1.54 | -3.88 | 23.09 | 24.91 | 28.00 | 1.99 | -2.17 | 26.25 | 28.56 | 33.21 | 1.93 |
Eisosomes | 5.85 | 3.97 | 6.20 | 2.02 | 5.86 | 5.98 | 11.21 | 10.40 | 13.66 | -4.96 | 7.93 | 11.60 | 11.22 | 13.08 | -2.23 |
Endoplasmic Reticulum | -5.18 | -5.22 | -5.52 | -2.92 | 4.03 | 2.93 | -2.58 | 1.80 | -1.36 | 5.01 | 1.76 | -5.16 | 0.19 | -1.97 | 5.71 |
Endosome | -0.99 | 3.53 | 5.79 | 11.62 | 2.10 | 4.84 | 10.67 | 24.74 | 19.87 | 12.62 | 5.91 | 11.33 | 25.74 | 23.05 | 12.93 |
Golgi | 2.45 | 5.24 | 5.22 | 11.15 | -0.19 | 6.49 | 16.25 | 15.87 | 11.85 | -0.09 | 7.70 | 17.08 | 16.89 | 14.85 | 0.08 |
Lipid Particles | 1.90 | 1.81 | 3.79 | 4.65 | 2.71 | 5.23 | 12.82 | 15.85 | 12.76 | 7.12 | 7.06 | 12.86 | 16.09 | 14.12 | 7.24 |
Mitochondria | 1.39 | 2.07 | 2.10 | 5.06 | 1.16 | -0.32 | 6.45 | 7.52 | 6.64 | 2.19 | 1.20 | 6.69 | 7.67 | 7.90 | 2.44 |
Mitotic Spindle | -5.83 | -3.99 | -6.12 | -4.73 | -0.14 | -1.68 | -13.12 | -9.34 | -8.59 | 4.83 | -4.28 | -13.70 | -10.99 | -9.28 | 4.36 |
None | -2.10 | -3.08 | -2.72 | -1.56 | 0.03 | 0.04 | 0.62 | -0.90 | -0.84 | -1.70 | 0.25 | 0.46 | -0.96 | -1.27 | -1.73 |
Nuclear Periphery | -3.56 | -9.66 | -4.96 | -4.23 | 0.11 | -1.22 | -10.15 | -10.16 | -9.76 | 6.03 | -2.45 | -10.90 | -10.85 | -9.77 | 5.65 |
Nuclear Periphery Foci | -0.49 | -5.42 | -4.23 | -4.23 | 1.25 | -0.85 | -8.63 | -7.24 | -6.14 | 5.83 | -2.15 | -9.73 | -7.86 | -7.05 | 5.22 |
Nucleolus | -1.38 | -1.92 | -0.92 | 0.50 | 1.73 | -1.87 | -9.23 | -2.10 | -0.93 | 2.27 | -2.83 | -7.97 | -2.31 | -0.58 | 2.97 |
Nucleus | 1.11 | -5.64 | -1.72 | -3.61 | 5.14 | 0.08 | -8.13 | -7.69 | -7.99 | -0.72 | 0.10 | -9.20 | -8.08 | -8.70 | 2.69 |
Peroxisomes | 5.90 | 4.54 | 4.88 | -0.59 | 0.34 | 1.04 | 1.69 | -0.99 | -1.74 | -2.23 | 3.91 | 3.16 | 0.74 | -2.28 | -1.94 |
Vacuole | -10.25 | -11.87 | -18.16 | -14.27 | -3.49 | -5.44 | -25.77 | -31.45 | -27.78 | -7.53 | -9.36 | -28.03 | -35.62 | -30.63 | -8.69 |
Vacuole Periphery | -5.74 | 0.83 | 3.67 | 8.47 | 3.61 | -1.59 | 7.39 | 2.66 | 3.39 | -0.27 | -2.66 | 7.49 | 3.23 | 4.60 | -0.01 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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