Standard name
Human Ortholog
Description ESCRT-I subunit required to stabilize ESCRT-I core complex oligomers; the ESCRT-I core complex (Stp22p, Vps28p, Srn2p) is involved in ubiquitin-dependent sorting of proteins into the endosome; deletion mutant is sensitive to rapamycin and nystatin

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0.05 0.08 0.07 0.05 0.07 0.07 0.1 0.1 0.09 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0 0 0 0 0 0
Cytoplasm 0.79 0.69 0.8 0.2 0.25 0.38 0.41 0.37 0.38 0.37 0.35 0.77 0.55 0.6 0.76 0.73 0.82 0.45 0.29 0.44 0.39 0.11 0.18
Endoplasmic Reticulum 0 0.08 0.05 0 0 0 0 0 0 0 0 0.09 0.05 0.06 0 0 0 0 0 0 0 0 0
Endosome 0.13 0.21 0 0.36 0.55 0.11 0.12 0.08 0.11 0.06 0.05 0.12 0.38 0.34 0.1 0.12 0.14 0.4 0.55 0.33 0.42 0.71 0.63
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0
Mitochondria 0 0 0.12 0.37 0.07 0.46 0.46 0.62 0.49 0.62 0.6 0 0 0 0 0 0 0 0 0.05 0.06 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0.06 0.07 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0.15 0.2 0.06 0.12 0.06 0.12 0.1 0.1 0 0 0 0 0.07 0.07 0 0 0 0 0 0
Vac/Vac Membrane 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.07 0 0 0 0.06 0.06 0.07 0.08
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 3 11 2 6 5 1 2 2 4 2 3 6 5 3 2 1 0 6 6 3 0 2 2
Bud 3 6 5 25 40 19 49 34 62 45 50 4 6 4 1 1 0 1 2 1 0 0 0
Bud Neck 0 0 4 0 1 0 1 0 3 0 1 0 0 1 0 1 0 2 1 0 0 0 0
Bud Site 0 0 0 0 0 0 2 1 7 6 7 0 0 0 0 0 0
Cell Periphery 1 0 0 0 0 0 1 1 4 2 2 1 1 0 1 2 2 0 0 0 0 0 0
Cytoplasm 274 132 86 59 133 144 292 193 243 169 200 131 117 171 45 59 23 173 64 67 29 27 45
Endoplasmic Reticulum 13 15 5 8 7 8 5 10 5 5 7 16 11 16 2 3 1 4 0 0 0 1 0
Endosome 44 39 0 106 297 42 83 42 68 28 30 21 82 98 6 10 4 154 121 50 31 187 156
Golgi 0 0 0 0 1 0 0 0 0 0 0 0 0 2 3 1 0 2 1 0 0 0 1
Mitochondria 10 0 13 110 40 173 328 324 320 286 343 3 0 1 0 3 0 12 2 8 4 10 7
Nucleus 1 0 0 0 1 3 4 3 1 1 2 0 2 2 0 0 0 0 0 0 0 1 0
Nuclear Periphery 0 0 0 0 0 1 2 1 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0
Nucleolus 1 0 0 5 4 3 3 3 4 1 3 0 1 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 17 40 6 16 3 22 4 15 0 0 2 0 0 0 0 1 0 0 0 1
SpindlePole 2 6 0 44 105 24 84 29 78 45 56 1 8 6 2 6 2 8 6 5 2 8 9
Vac/Vac Membrane 17 6 2 3 23 12 25 11 21 12 11 7 7 11 3 6 0 10 6 8 4 18 19
Unique Cell Count 348 190 108 298 537 375 720 521 647 462 573 170 214 286 59 81 28 385 219 154 75 264 249
Labelled Cell Count 369 215 117 383 697 436 897 657 842 606 730 190 240 318 65 93 32 385 219 154 75 264 249


Endosome

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 6.0 6.8 4.0 4.5 5.1 3.9 4.2 3.9 4.1 3.8 3.8 6.2 6.5 6.4 8.1 8.2 8.0 5.8 6.5 7.0
Std Deviation (1e-4) 0.8 1.0 0.7 1.0 1.2 0.9 1.1 0.9 0.9 0.7 0.8 0.9 1.0 0.9 1.3 1.3 1.8 0.9 0.8 1.7
Intensity Change (Log2) 0.19 0.34 -0.03 0.06 -0.04 0.06 -0.06 -0.06 0.64 0.71 0.68 1.03 1.04 1.01 0.54 0.7 0.81


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 1.0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm -11.1 -11.0 -7.6 -7.6 -8.1 -8.2 -8.1 -8.6 -0.5 -4.4 -3.7 -0.5 -1.1 0.3
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 1.5 0.2 0.4 0 0 0
Endosome 0 10.5 0 0 0 0 0 0 3.8 7.5 7.0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 -1.6 0 0 0 0 0 0 -3.6 0 0 0 -2.0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 2.9 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 5.0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 1.1487 1.6424 1.1803 1.3014 1.0736 1.3663 0.5508 0.9274 0.8636 0.7656 0.4084 0.8153 2.4355 2.8293 2.3372 3.7346 2.5305 2.8394
Actin 0.0243 0.0018 0.0107 0.046 0.0001 0.0022 0.0236 0.0108 0.0064 0.0001 0.0123 0.0034 0.02 0.0002 0.0004 0.0453 0.0163 0.0101
Bud 0.0002 0.0001 0.0002 0.0002 0.0001 0.0002 0.001 0.004 0.0003 0.0002 0.0215 0.0002 0.0004 0.0001 0.0002 0.0016 0.0002 0.0001
Bud Neck 0.0031 0.0003 0.0004 0.0042 0.0003 0.0043 0.0004 0.0004 0.0026 0.0003 0.0033 0.0007 0.0005 0.0002 0.0002 0.0003 0.0005 0.0003
Bud Periphery 0.0003 0.0001 0.0002 0.0002 0 0.0002 0.0015 0.0025 0.0002 0.0001 0.0208 0.0002 0.0006 0.0001 0.0001 0.0019 0.0003 0.0001
Bud Site 0.0012 0.0013 0.0007 0.0007 0 0.0015 0.0026 0.0088 0.0009 0.0004 0.018 0.0003 0.0015 0.0009 0.0003 0.0099 0.0004 0.0003
Cell Periphery 0.0002 0.0001 0.0001 0.0001 0 0.0001 0.0003 0.0002 0.0001 0 0.0004 0 0.0001 0.0003 0.0001 0.0001 0.0001 0
Cytoplasm 0.3723 0.422 0.5121 0.362 0.6067 0.4025 0.3138 0.4975 0.4226 0.5353 0.3581 0.4164 0.2525 0.4915 0.477 0.3241 0.2535 0.3979
Cytoplasmic Foci 0.1884 0.1927 0.1334 0.2085 0.0946 0.22 0.1934 0.1526 0.1369 0.0789 0.2201 0.2353 0.1657 0.1276 0.0952 0.1114 0.1429 0.2294
Eisosomes 0.0002 0 0.0003 0.0005 0 0 0.0006 0 0.0001 0 0 0 0.0002 0 0 0.0002 0.0001 0
Endoplasmic Reticulum 0.0111 0.0066 0.0068 0.0088 0.0056 0.007 0.0107 0.0054 0.0076 0.0036 0.0098 0.0038 0.0073 0.0077 0.0071 0.0145 0.0019 0.0081
Endosome 0.2298 0.2032 0.1835 0.2296 0.1694 0.2113 0.2199 0.1623 0.2068 0.1954 0.2096 0.2436 0.1553 0.154 0.1544 0.1584 0.2255 0.193
Golgi 0.014 0.0067 0.0067 0.0089 0.0025 0.0073 0.0181 0.0086 0.0234 0.0195 0.0415 0.0144 0.0185 0.006 0.0047 0.0052 0.0152 0.0068
Lipid Particles 0.0137 0.0055 0.0027 0.0055 0.0004 0.0077 0.0132 0.0015 0.0076 0.004 0.0086 0.0036 0.0253 0.0046 0.0033 0.0036 0.0211 0.0076
Mitochondria 0.0025 0.0006 0.0016 0.0013 0.0008 0.0014 0.0183 0.0009 0.014 0.0013 0.0112 0.0018 0.0048 0.0006 0.0016 0.005 0.0054 0.0009
None 0.0972 0.1381 0.12 0.0916 0.1107 0.1069 0.1431 0.1262 0.1299 0.1431 0.0087 0.0536 0.2705 0.1897 0.2376 0.2929 0.2189 0.1193
Nuclear Periphery 0.0078 0.0033 0.0046 0.004 0.0014 0.0042 0.0113 0.0021 0.0104 0.0013 0.0037 0.001 0.0057 0.0018 0.0017 0.0021 0.0078 0.0063
Nucleolus 0.0012 0.0002 0.0001 0.0001 0.0001 0.0003 0.0006 0.0001 0.0004 0.0001 0.0004 0.0001 0.0003 0.0002 0.0002 0.0001 0.0024 0.0001
Nucleus 0.0042 0.0021 0.002 0.0015 0.0015 0.0019 0.0049 0.0015 0.0091 0.0008 0.0024 0.0012 0.0012 0.0015 0.0008 0.0009 0.0179 0.0017
Peroxisomes 0.0083 0.0026 0.0035 0.0129 0.0004 0.0076 0.0062 0.0034 0.0094 0.0035 0.0184 0.0135 0.0548 0.0014 0.0013 0.0131 0.0554 0.0028
Punctate Nuclear 0.0115 0.0027 0.0024 0.002 0.0007 0.0067 0.0056 0.0021 0.0018 0.0005 0.0021 0.0017 0.0099 0.0013 0.0006 0.001 0.0086 0.005
Vacuole 0.0068 0.0086 0.0066 0.009 0.0035 0.0055 0.0089 0.0079 0.0071 0.0089 0.0209 0.0042 0.0038 0.0093 0.0102 0.0061 0.0041 0.0078
Vacuole Periphery 0.0017 0.0013 0.0014 0.0022 0.0012 0.0012 0.0019 0.0011 0.0022 0.0027 0.0081 0.0011 0.001 0.001 0.0029 0.0023 0.0014 0.0022

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 10.8177 12.8938 19.1355 21.7052 16.3632 7.2078 9.7777 14.2984 16.6497 15.72
Translational Efficiency 1.2417 0.7503 0.8343 0.4824 0.7864 1.7493 0.9467 0.9431 1.0788 1.3259

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
145 918 130 243 1659 1427 1197 1176 1804 2345 1327 1419

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 654.39 804.15 1045.28 959.82 753.64 801.30 933.34 891.53 745.66 802.42 944.31 903.22
Standard Deviation 80.28 122.19 128.50 125.81 100.38 107.51 107.04 123.39 102.53 113.50 114.28 126.45
Intensity Change Log 2 0.297314 0.675667 0.552613 0.088467 0.308527 0.242408 0.189300 0.490816 0.394900

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000842 0.000983 0.005343 0.005565 0.000743 0.000557 0.000922 0.002991 0.000751 0.000723 0.001355 0.003432
Bud Neck 0.028615 0.012712 0.017067 0.012395 0.021085 0.016641 0.013501 0.009864 0.021691 0.015103 0.013851 0.010297
Bud Site 0.010009 0.013596 0.023468 0.048348 0.006523 0.008359 0.021955 0.032618 0.006803 0.010409 0.022103 0.035312
Cell Periphery 0.000092 0.000055 0.000233 0.000101 0.000090 0.000076 0.000054 0.000067 0.000090 0.000068 0.000072 0.000073
Cytoplasm 0.087559 0.157387* 0.115784* 0.142524* 0.109984* 0.129868* 0.129384* 0.208318* 0.108181* 0.140641* 0.128052* 0.197051*
Cytoplasmic Foci 0.452600* 0.369676* 0.132566 0.193509* 0.356479* 0.388501* 0.218655* 0.218283* 0.364205* 0.381132* 0.210221* 0.214040*
Eisosomes 0.000284 0.000078 0.000135 0.000068 0.000251 0.000135 0.000039 0.000056 0.000254 0.000113 0.000048 0.000058
Endoplasmic Reticulum 0.000326 0.001264 0.010588 0.002655 0.001455 0.000883 0.001789 0.001080 0.001365 0.001032 0.002651 0.001350
Endosome 0.195341 0.219329* 0.152114 0.128636 0.283532* 0.240441* 0.244030* 0.135009 0.276443* 0.232176* 0.235026* 0.133918
Golgi 0.032914 0.018219 0.001780 0.002812 0.035838 0.020662 0.004493 0.006044 0.035603 0.019706 0.004227 0.005490
Lipid Particles 0.029992 0.019835 0.023856 0.012693 0.045622 0.029661 0.012600 0.006217 0.044366 0.025814 0.013703 0.007326
Mitochondria 0.015229 0.005462 0.000694 0.000627 0.009358 0.009963 0.002469 0.001787 0.009830 0.008201 0.002295 0.001589
Mitotic Spindle 0.001684 0.013101 0.043864 0.059508 0.003762 0.006175 0.065367 0.043374 0.003595 0.008887 0.063260 0.046137
None 0.000167 0.000352 0.000493 0.000450 0.000628 0.000422 0.000383 0.001249 0.000591 0.000395 0.000394 0.001112
Nuclear Periphery 0.000124 0.000388 0.001813 0.001156 0.000229 0.000276 0.001759 0.000772 0.000221 0.000320 0.001764 0.000838
Nuclear Periphery Foci 0.001303 0.001574 0.019961 0.011924 0.000771 0.001026 0.010456 0.003645 0.000814 0.001241 0.011387 0.005063
Nucleolus 0.000924 0.001487 0.004707 0.001346 0.000577 0.001077 0.001905 0.000840 0.000605 0.001237 0.002179 0.000927
Nucleus 0.014603 0.010098 0.121776 0.017274 0.009621 0.009551 0.014812 0.016649 0.010021 0.009765 0.025291 0.016756
Peroxisomes 0.085068 0.024618 0.039639 0.052584 0.031656 0.029132 0.034635 0.053689 0.035949 0.027365 0.035125 0.053500
Vacuole 0.040846 0.125261 0.282567* 0.305004* 0.078264 0.102446 0.218830* 0.254268* 0.075256 0.111377 0.225074* 0.262956*
Vacuole Periphery 0.001480 0.004527 0.001550 0.000823 0.003531 0.004149 0.001963 0.003180 0.003366 0.004297 0.001922 0.002776

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -0.34 -2.11 -2.21 -2.11 0.55 1.43 -0.69 -2.88 -3.38 -2.64 0.17 -2.01 -3.59 -3.65 -2.38
Bud Neck 3.03 1.69 3.38 1.20 2.27 2.22 2.23 5.69 4.38 4.49 3.72 2.57 6.40 4.24 5.00
Bud Site -1.29 -3.72 -4.48 -4.25 -1.50 -1.98 -8.31 -7.82 -6.90 -1.62 -4.41 -9.08 -9.26 -7.49 -2.27
Cell Periphery 2.69 -5.34 -0.15 -4.15 5.55 1.49 4.41 4.40 2.53 -0.19 2.90 1.86 3.79 0.87 2.21
Cytoplasm -5.24 -2.20 -4.23 -0.21 -1.36 -3.00 -5.32 -12.68 -10.06 -7.20 -5.56 -5.65 -13.01 -8.79 -6.98
Cytoplasmic Foci 4.30 15.07 14.88 18.46 -1.54 -3.88 23.09 24.91 28.00 1.99 -2.17 26.25 28.56 33.21 1.93
Eisosomes 5.85 3.97 6.20 2.02 5.86 5.98 11.21 10.40 13.66 -4.96 7.93 11.60 11.22 13.08 -2.23
Endoplasmic Reticulum -5.18 -5.22 -5.52 -2.92 4.03 2.93 -2.58 1.80 -1.36 5.01 1.76 -5.16 0.19 -1.97 5.71
Endosome -0.99 3.53 5.79 11.62 2.10 4.84 10.67 24.74 19.87 12.62 5.91 11.33 25.74 23.05 12.93
Golgi 2.45 5.24 5.22 11.15 -0.19 6.49 16.25 15.87 11.85 -0.09 7.70 17.08 16.89 14.85 0.08
Lipid Particles 1.90 1.81 3.79 4.65 2.71 5.23 12.82 15.85 12.76 7.12 7.06 12.86 16.09 14.12 7.24
Mitochondria 1.39 2.07 2.10 5.06 1.16 -0.32 6.45 7.52 6.64 2.19 1.20 6.69 7.67 7.90 2.44
Mitotic Spindle -5.83 -3.99 -6.12 -4.73 -0.14 -1.68 -13.12 -9.34 -8.59 4.83 -4.28 -13.70 -10.99 -9.28 4.36
None -2.10 -3.08 -2.72 -1.56 0.03 0.04 0.62 -0.90 -0.84 -1.70 0.25 0.46 -0.96 -1.27 -1.73
Nuclear Periphery -3.56 -9.66 -4.96 -4.23 0.11 -1.22 -10.15 -10.16 -9.76 6.03 -2.45 -10.90 -10.85 -9.77 5.65
Nuclear Periphery Foci -0.49 -5.42 -4.23 -4.23 1.25 -0.85 -8.63 -7.24 -6.14 5.83 -2.15 -9.73 -7.86 -7.05 5.22
Nucleolus -1.38 -1.92 -0.92 0.50 1.73 -1.87 -9.23 -2.10 -0.93 2.27 -2.83 -7.97 -2.31 -0.58 2.97
Nucleus 1.11 -5.64 -1.72 -3.61 5.14 0.08 -8.13 -7.69 -7.99 -0.72 0.10 -9.20 -8.08 -8.70 2.69
Peroxisomes 5.90 4.54 4.88 -0.59 0.34 1.04 1.69 -0.99 -1.74 -2.23 3.91 3.16 0.74 -2.28 -1.94
Vacuole -10.25 -11.87 -18.16 -14.27 -3.49 -5.44 -25.77 -31.45 -27.78 -7.53 -9.36 -28.03 -35.62 -30.63 -8.69
Vacuole Periphery -5.74 0.83 3.67 8.47 3.61 -1.59 7.39 2.66 3.39 -0.27 -2.66 7.49 3.23 4.60 -0.01
Standard name
Human Ortholog
Description ESCRT-I subunit required to stabilize ESCRT-I core complex oligomers; the ESCRT-I core complex (Stp22p, Vps28p, Srn2p) is involved in ubiquitin-dependent sorting of proteins into the endosome; deletion mutant is sensitive to rapamycin and nystatin
Localization
Cell Percentages endosome (31%), cytoplasm (20%), mixed (20%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Mvb12

Mvb12


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Mvb12-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available