Standard name
Human Ortholog
Description Cytoplasmic thioredoxin isoenzyme; part of thioredoxin system which protects cells against oxidative and reductive stress; forms LMA1 complex with Pbi2p; acts as a cofactor for Tsa1p; required for ER-Golgi transport and vacuole inheritance; with Trx1p, facilitates mitochondrial import of small Tims Tim9p, Tim10p, Tim13p by maintaining them in reduced form; abundance increases under DNA replication stress; TRX2 has a paralog, TRX1, that arose from the whole genome duplication

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.99 0.99 0.99 0.99 0.98 0.96 0.91 0.89 0.89 0.94 0.87 0.98 0.99 1.0 1.0 1.0 1.0 0.95 0.94 0.97 0.85 0.91 0.86
Endoplasmic Reticulum 0 0 0 0 0 0 0.1 0.07 0.06 0.05 0.1 0 0 0 0 0.05 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 1 0 0 0 0 0 2 2 2 2 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 2 0 0 1 0 0 1 0 0 2 1 0 0 0 0 1 4 4 0 1 2 7
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 2 1 0 1 0 0 0 4 5 0 8 0 0 1 2 5 2 0 0 0 0 0 0
Cytoplasm 386 349 161 138 185 161 134 154 152 162 228 257 265 285 313 351 261 370 332 144 127 230 211
Endoplasmic Reticulum 0 2 1 0 4 5 15 12 11 9 25 5 3 0 5 18 8 1 2 0 6 1 3
Endosome 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 1 0 1 0 1 0 1 0 0 0 0 0 0 2 0
Mitochondria 0 0 0 0 0 1 0 0 0 1 3 0 0 0 1 0 0 0 1 0 0 0 0
Nucleus 0 0 1 0 0 0 0 2 2 2 0 1 0 0 0 0 0 5 5 2 3 5 7
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 2 0 0 2 0 0 0 2 2 0 4 1 1 1 0 0 1 2 1 0 2 6 8
Unique Cell Count 388 354 163 139 189 167 148 174 170 173 263 262 269 286 314 351 262 390 353 149 150 253 246
Labelled Cell Count 390 356 163 141 190 167 149 177 175 176 274 265 270 287 322 374 273 390 353 149 150 253 246


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 82.3 75.3 49.9 58.3 58.4 56.4 52.9 54.3 53.0 50.6 52.2 91.0 99.7 99.3 81.8 79.9 80.7 58.5 68.1 70.7
Std Deviation (1e-4) 21.8 23.1 12.2 14.7 13.7 12.9 17.1 18.7 17.1 13.5 18.0 27.3 30.9 30.9 21.8 21.0 22.5 18.4 20.9 29.9
Intensity Change (Log2) 0.22 0.23 0.18 0.09 0.12 0.09 0.02 0.07 0.87 1.0 0.99 0.72 0.68 0.69 0.23 0.45 0.5


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.4 -0.6 -1.4 -3.3 -3.8 -3.6 -2.4 -4.3 -0.5 -0.2 1.1 1.2 2.1 1.0
Endoplasmic Reticulum 0 0 0 3.8 3.0 2.9 0 3.7 0 0 0 0 2.5 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration -0.8733 -0.492 -0.584 -0.9632 -0.754 -0.4702 0.9365 1.7869 1.3937 1.3522 1.377 1.6734 -0.6385 -0.2346 -0.1238 -0.159 0.0101 -0.2029
Actin 0.0205 0.0003 0.0003 0.0004 0.0047 0.0014 0.0001 0.0001 0.0001 0 0.0001 0.0001 0.0023 0.0001 0.0019 0.0047 0.0011 0.016
Bud 0.0062 0.0041 0.0048 0.0075 0.0005 0.0021 0 0 0.0002 0 0 0.0009 0.0004 0.0011 0.0001 0.0003 0.0056 0.0001
Bud Neck 0.002 0.0002 0.0011 0.0004 0.0134 0.0005 0 0 0 0 0 0.0002 0.0014 0.0001 0.0002 0.0006 0.002 0.0003
Bud Periphery 0.0033 0.0034 0.0035 0.0103 0.0006 0.0007 0 0.0002 0.0002 0 0 0.0002 0.0009 0.0012 0.0004 0.0005 0.0294 0.0001
Bud Site 0.0135 0.0155 0.0048 0.0081 0.0011 0.0011 0 0.0001 0.0004 0 0 0.0002 0.0224 0.0044 0.0003 0.0009 0.0047 0.0002
Cell Periphery 0.0004 0.0006 0.0001 0.0003 0.0001 0.0001 0 0.0002 0.0001 0 0 0 0.001 0.0001 0.0003 0.0002 0.0007 0
Cytoplasm 0.0127 0.0121 0.0094 0.0129 0.0202 0.0205 0.0354 0.0321 0.0297 0.0096 0.019 0.0459 0.0314 0.0341 0.0406 0.0488 0.0267 0.0842
Cytoplasmic Foci 0.0213 0.0016 0.0088 0.0022 0.0222 0.0086 0.001 0.0023 0.0011 0.0004 0.0014 0.0037 0.0614 0.0028 0.0097 0.0083 0.0129 0.0082
Eisosomes 0.0003 0.0002 0.0001 0.0006 0.0002 0.0001 0.0002 0.0003 0.0001 0.0001 0.0001 0.0001 0.0014 0.0001 0.0004 0.0009 0.0004 0.0002
Endoplasmic Reticulum 0.001 0.0002 0.0002 0.0016 0.001 0.0004 0.0002 0.0002 0.0003 0.0001 0.0001 0.0002 0.0019 0.0004 0.0025 0.0025 0.0029 0.0004
Endosome 0.0054 0.0001 0.0001 0.0003 0.0035 0.0031 0.0001 0.0004 0.0002 0 0 0.0001 0.0546 0.0026 0.0143 0.0037 0.0877 0.0044
Golgi 0.0015 0 0 0.0001 0.0009 0.0016 0 0.0001 0 0 0 0 0.0112 0.0007 0.0063 0.0004 0.0051 0.01
Lipid Particles 0.0017 0.0001 0.0003 0.0004 0.0016 0.0012 0 0.0001 0.0002 0 0.0001 0.0001 0.0147 0.0022 0.0116 0.001 0.0043 0.0049
Mitochondria 0.0018 0.0005 0.0003 0.0004 0.0003 0.0011 0 0.0008 0.0003 0 0.0001 0.0002 0.0162 0.0002 0.0235 0.0004 0.0158 0.0043
None 0.8935 0.945 0.9547 0.9462 0.9135 0.9389 0.9626 0.9623 0.9643 0.9896 0.9785 0.9458 0.7558 0.948 0.8711 0.9209 0.7731 0.8576
Nuclear Periphery 0.0034 0.0003 0.0003 0.0004 0.0027 0.0013 0 0 0.0003 0 0.0001 0.0001 0.0024 0.0001 0.0022 0.0012 0.0016 0.0001
Nucleolus 0.0007 0.0011 0.0011 0.0008 0.0011 0.0009 0 0.0001 0.0002 0 0 0.0002 0.0031 0.0001 0.0002 0.0003 0.0021 0
Nucleus 0.0012 0.0066 0.0018 0.0026 0.0017 0.0021 0.0001 0.0001 0.0006 0 0.0001 0.0008 0.0029 0.0002 0.0013 0.0006 0.0019 0.0001
Peroxisomes 0.002 0.0002 0.0001 0.0002 0.0006 0.0007 0 0.0001 0.0001 0 0.0001 0.0001 0.0072 0.0005 0.0072 0.001 0.0011 0.0083
Punctate Nuclear 0.0042 0.0073 0.0079 0.0038 0.0096 0.0131 0.0001 0.0001 0.0013 0 0.0002 0.001 0.0015 0.0002 0.0009 0.0015 0.0011 0.0003
Vacuole 0.0026 0.0003 0.0002 0.0005 0.0005 0.0003 0.0001 0.0003 0.0003 0 0 0.0001 0.0047 0.0006 0.0035 0.0011 0.0159 0.0001
Vacuole Periphery 0.0007 0 0 0.0001 0.0001 0.0001 0 0.0001 0.0001 0 0 0 0.0012 0 0.0016 0.0003 0.0038 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 446.6161 535.4614 594.8413 770.076 554.0783 448.1193 683.8793 728.6861 734.5233 644.5424
Translational Efficiency 2.3186 2.3509 2.5578 1.8871 2.7477 3.043 2.3771 2.7943 2.8368 2.4659

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency
Standard name
Human Ortholog
Description Cytoplasmic thioredoxin isoenzyme; part of thioredoxin system which protects cells against oxidative and reductive stress; forms LMA1 complex with Pbi2p; acts as a cofactor for Tsa1p; required for ER-Golgi transport and vacuole inheritance; with Trx1p, facilitates mitochondrial import of small Tims Tim9p, Tim10p, Tim13p by maintaining them in reduced form; abundance increases under DNA replication stress; TRX2 has a paralog, TRX1, that arose from the whole genome duplication
Localization
Cell Percentages
Cell Cycle Regulation No
Subcompartmental Group N/A

Trx2

Trx2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Trx2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available