Standard name | |
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Human Ortholog | |
Description | Major karyopherin; involved in export of proteins, RNAs, and ribosomal subunits from the nucleus; exportin |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 1 | 0 | 0 | 3 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | – | 0 | 0 | 0 | 0 | 1 | – | 0 | 0 | 0 | 2 | 1 | 0 | 1 | 1 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 1 | 3 | 1 | 2 | – | 2 | 4 | 0 | 0 | 2 | – | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 8 | 4 | 10 | 16 | 22 | 14 | 24 | 19 | 15 | – | 17 | 11 | 3 | 12 | 14 | – | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 |
Endoplasmic Reticulum | 1 | 0 | 0 | 0 | 1 | 2 | 0 | 1 | 1 | – | 0 | 0 | 1 | 0 | 2 | – | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | – | 0 | 1 | 0 | 3 | 7 | – | 0 | 0 | 0 | 0 | 2 | 1 | 0 | 5 |
Golgi | 1 | 0 | 0 | 0 | 1 | 0 | 1 | 1 | 0 | – | 0 | 0 | 0 | 0 | 0 | – | 1 | 2 | 0 | 0 | 0 | 0 | 0 | 3 |
Mitochondria | 0 | 0 | 0 | 3 | 1 | 6 | 8 | 13 | 11 | – | 12 | 15 | 0 | 0 | 3 | – | 0 | 0 | 0 | 0 | 1 | 3 | 0 | 2 |
Nucleus | 85 | 145 | 83 | 110 | 107 | 96 | 163 | 190 | 106 | – | 99 | 121 | 38 | 49 | 20 | – | 10 | 1 | 82 | 152 | 66 | 57 | 65 | 37 |
Nuclear Periphery | 2 | 4 | 8 | 27 | 27 | 20 | 29 | 19 | 14 | – | 11 | 19 | 11 | 24 | 42 | – | 0 | 0 | 23 | 9 | 20 | 1 | 3 | 5 |
Nucleolus | 12 | 5 | 7 | 19 | 38 | 51 | 49 | 44 | 21 | – | 33 | 34 | 12 | 37 | 52 | – | 0 | 1 | 31 | 31 | 30 | 13 | 10 | 18 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 1 | – | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 |
Vac/Vac Membrane | 31 | 54 | 27 | 17 | 25 | 30 | 28 | 41 | 13 | – | 9 | 25 | 32 | 63 | 38 | – | 6 | 11 | 18 | 33 | 26 | 28 | 57 | 97 |
Unique Cell Count | 131 | 193 | 126 | 175 | 201 | 200 | 276 | 307 | 171 | 171 | 201 | 85 | 165 | 158 | 17 | 16 | 159 | 232 | 152 | 111 | 143 | 175 | ||
Labelled Cell Count | 140 | 213 | 135 | 192 | 222 | 222 | 306 | 329 | 183 | 183 | 231 | 97 | 188 | 185 | 17 | 19 | 159 | 232 | 152 | 111 | 143 | 175 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 25.7 | 21.9 | 18.6 | 17.8 | 20.5 | 18.5 | 18.3 | 15.9 | 16.9 | – | 16.0 | 15.4 | 20.5 | 19.5 | 17.5 | – | 33.9 | 30.2 | 20.0 | 22.6 | 25.7 |
Std Deviation (1e-4) | 7.4 | 5.4 | 6.7 | 6.2 | 9.0 | 7.2 | 7.6 | 6.1 | 7.4 | – | 6.7 | 6.9 | 5.2 | 6.1 | 5.6 | – | 13.0 | 10.5 | 5.5 | 6.3 | 6.8 |
Intensity Change (Log2) | – | – | – | -0.07 | 0.14 | -0.01 | -0.03 | -0.23 | -0.14 | – | -0.22 | -0.27 | 0.14 | 0.06 | -0.09 | – | 0.86 | 0.7 | 0.11 | 0.28 | 0.47 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 |
Cytoplasm | 0.4 | 0.9 | -0.3 | 0.3 | -0.7 | 0.3 | – | 0.6 | -0.9 | -1.3 | -0.2 | 0.3 | – | 0 | 0 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 |
Mitochondria | 0 | 0 | 0 | 0 | 0 | 0 | – | 3.0 | 3.1 | 0 | 0 | 0 | – | 0 | 0 |
Nucleus | -0.5 | -2.3 | -3.2 | -1.3 | -0.8 | -0.7 | – | -1.4 | -1.0 | -3.0 | -6.1 | -9.3 | – | -0.6 | -4.6 |
Nuclear Periphery | 2.4 | 2.0 | 1.1 | 1.3 | -0.1 | 0.6 | – | 0 | 1.0 | 1.6 | 2.2 | 4.4 | – | 0 | 0 |
Nucleolus | 1.6 | 3.4 | 4.6 | 3.3 | 2.6 | 2.0 | – | 3.4 | 3.0 | 2.1 | 4.0 | 5.6 | – | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 |
Vacuole | -2.8 | -2.2 | -1.5 | -3.1 | -2.1 | -3.4 | – | -4.2 | -2.2 | 2.6 | 3.1 | 0.5 | – | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 19.5745 | 25.3239 | 22.7358 | 21.5222 | 21.2287 | 22.7062 | 22.6759 | 28.2168 | 23.5842 | 19.5951 | 21.9032 | 24.9246 | 25.1905 | 24.8817 | 23.9287 | 22.5404 | 22.4833 | 23.3519 |
Actin | 0.0673 | 0.0003 | 0.0015 | 0.0217 | 0.0332 | 0.0258 | 0.0106 | 0.0003 | 0.007 | 0.0011 | 0.003 | 0.0006 | 0.0005 | 0.0009 | 0.0007 | 0.0004 | 0.0003 | 0.0005 |
Bud | 0.0009 | 0 | 0.0005 | 0.0001 | 0.0004 | 0.0002 | 0.0002 | 0 | 0.0004 | 0.0002 | 0.0003 | 0 | 0.0001 | 0.0002 | 0.0057 | 0 | 0 | 0 |
Bud Neck | 0.0038 | 0.0001 | 0.0002 | 0.0002 | 0.0011 | 0.0019 | 0.0002 | 0.0001 | 0.0003 | 0.0002 | 0.0005 | 0.0007 | 0 | 0.0021 | 0.0001 | 0.0001 | 0.0002 | 0.0005 |
Bud Periphery | 0.0017 | 0.0001 | 0.001 | 0.0002 | 0.0009 | 0.0009 | 0.0004 | 0 | 0.0006 | 0.0004 | 0.0005 | 0.0002 | 0.0002 | 0.0011 | 0.0018 | 0.0001 | 0.0001 | 0.0002 |
Bud Site | 0.0082 | 0 | 0.0003 | 0.0002 | 0.001 | 0.0002 | 0.0005 | 0 | 0.0007 | 0.0006 | 0.0005 | 0 | 0.0001 | 0.0002 | 0.0046 | 0 | 0 | 0 |
Cell Periphery | 0.002 | 0.0001 | 0.0004 | 0.0003 | 0.001 | 0.0004 | 0.0003 | 0.0001 | 0.0003 | 0.0004 | 0.0003 | 0.0001 | 0.0001 | 0.0009 | 0.0001 | 0.0001 | 0.0001 | 0.0001 |
Cytoplasm | 0.0049 | 0.0005 | 0.0009 | 0.0015 | 0.0005 | 0.0005 | 0.0043 | 0.0003 | 0.0027 | 0.002 | 0.0014 | 0.0005 | 0.0002 | 0.0006 | 0.0007 | 0.0011 | 0.0006 | 0.0003 |
Cytoplasmic Foci | 0.0297 | 0 | 0 | 0.0181 | 0.0023 | 0.0005 | 0.0118 | 0 | 0.0071 | 0.002 | 0.0146 | 0.0045 | 0.0003 | 0 | 0 | 0 | 0.0001 | 0 |
Eisosomes | 0.0007 | 0 | 0 | 0.0001 | 0.0004 | 0.0005 | 0.0001 | 0 | 0.0002 | 0.0001 | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0.0061 | 0.0002 | 0.0005 | 0.0005 | 0.0003 | 0.0015 | 0.0029 | 0.0001 | 0.0015 | 0.0014 | 0.001 | 0.0004 | 0.0002 | 0.0006 | 0.0002 | 0.0003 | 0.0003 | 0.0003 |
Endosome | 0.0255 | 0 | 0.0003 | 0.002 | 0.0014 | 0.0028 | 0.0102 | 0.0002 | 0.0085 | 0.0066 | 0.0108 | 0.0005 | 0.0002 | 0.0003 | 0.0001 | 0.0001 | 0.0004 | 0.0001 |
Golgi | 0.0122 | 0 | 0 | 0.0019 | 0.0049 | 0.0035 | 0.0036 | 0.0001 | 0.0035 | 0.001 | 0.0073 | 0.0001 | 0 | 0 | 0 | 0 | 0.0001 | 0 |
Lipid Particles | 0.0183 | 0 | 0.0001 | 0.0052 | 0.0146 | 0.0028 | 0.0162 | 0.0008 | 0.0047 | 0.0194 | 0.0181 | 0.0026 | 0.0004 | 0 | 0 | 0 | 0.0016 | 0 |
Mitochondria | 0.0147 | 0.0014 | 0.0047 | 0.0053 | 0.0429 | 0.0066 | 0.0056 | 0.0039 | 0.0164 | 0.0386 | 0.0268 | 0.0015 | 0.0096 | 0.0014 | 0.0016 | 0.001 | 0.0024 | 0.0014 |
None | 0.0037 | 0 | 0.0002 | 0.0018 | 0.0003 | 0.0026 | 0.0029 | 0 | 0.0014 | 0.0007 | 0.0004 | 0.0002 | 0.0001 | 0 | 0.0002 | 0 | 0.0001 | 0 |
Nuclear Periphery | 0.325 | 0.3114 | 0.3757 | 0.3247 | 0.0636 | 0.2855 | 0.3769 | 0.3355 | 0.4535 | 0.3113 | 0.2988 | 0.3225 | 0.324 | 0.3316 | 0.5473 | 0.4199 | 0.2973 | 0.3309 |
Nucleolus | 0.0125 | 0.0028 | 0.0086 | 0.0128 | 0.0374 | 0.0211 | 0.0129 | 0.0054 | 0.0089 | 0.0088 | 0.0185 | 0.005 | 0.0062 | 0.0055 | 0.002 | 0.0051 | 0.0106 | 0.0051 |
Nucleus | 0.4018 | 0.6744 | 0.5923 | 0.5779 | 0.7721 | 0.6211 | 0.5107 | 0.6383 | 0.4581 | 0.5717 | 0.5774 | 0.6514 | 0.6455 | 0.6224 | 0.4257 | 0.5628 | 0.6739 | 0.651 |
Peroxisomes | 0.0191 | 0 | 0 | 0.0067 | 0.0029 | 0.0006 | 0.0032 | 0 | 0.0013 | 0.0006 | 0.0021 | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0 |
Punctate Nuclear | 0.0081 | 0.0001 | 0.0005 | 0.0028 | 0.0005 | 0.0019 | 0.0089 | 0.0003 | 0.0022 | 0.0029 | 0.0027 | 0.0022 | 0.0034 | 0.0005 | 0.0005 | 0.0004 | 0.0007 | 0.0002 |
Vacuole | 0.0111 | 0.0012 | 0.0024 | 0.0015 | 0.0046 | 0.0034 | 0.0049 | 0.001 | 0.005 | 0.0075 | 0.003 | 0.0009 | 0.0009 | 0.0239 | 0.0006 | 0.002 | 0.0013 | 0.0011 |
Vacuole Periphery | 0.0226 | 0.0073 | 0.0096 | 0.0144 | 0.0138 | 0.0156 | 0.0126 | 0.0135 | 0.0157 | 0.0225 | 0.0119 | 0.0063 | 0.0079 | 0.0077 | 0.008 | 0.0065 | 0.0101 | 0.0082 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 124.4084 | 98.4861 | 102.3133 | 136.9868 | 110.7571 | 107.7384 | 106.3965 | 113.8421 | 108.4325 | 127.5256 |
Translational Efficiency | 1.1861 | 1.3931 | 1.2515 | 0.9176 | 1.1139 | 1.122 | 1.0227 | 0.8283 | 0.9502 | 0.8807 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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