ORF
Human Ortholog
Description Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF MRPL9/YGR220C
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 – 0 0 0.05 0 0.06 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0.06 0 0.06 0 – 0 0 0 0 0 0.06 0.12 0.16 0.12 0.14 0.12 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.1 0 0.34 0.27 0.05 0.31 – 0.3 0.46 0.32 0.48 0.44 0 0 0 0.14 0.14 0.07 0 0 0 0 0 0
Nucleus 0.93 0.92 0.81 0.82 0.83 0.75 – 0.65 0.65 0.67 0.64 0.63 0.85 0.81 0.75 0.69 0.67 0.69 0.88 0.78 0.74 0.69 0.66 0.61
Nuclear Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.14 0.17 0.18 0.26 0.1 0.22 – 0.36 0.3 0.33 0.33 0.37 0.18 0.15 0.1 0.07 0.07 0.08 0.06 0.13 0.12 0.17 0.17 0.22
Peroxisomes 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0.11 0.06 0.09 0 0 0 0.05 0.07 0.08
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0
Bud 0 0 4 1 0 1 – 3 1 2 3 1 0 0 0 0 0 1 1 2 0 0 1 2
Bud Neck 0 0 0 0 0 0 – 0 0 1 0 0 0 0 0 0 0 0 0 0 1 0 0 0
Bud Site 1 1 7 2 5 11 – 11 8 14 11 19 0 0 0 1 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 – 1 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0
Cytoplasm 3 11 15 6 18 12 – 15 8 11 10 5 19 48 56 18 15 12 0 1 0 0 0 0
Endoplasmic Reticulum 0 0 1 1 0 0 – 0 0 0 0 0 1 0 0 5 4 2 0 1 3 1 1 0
Endosome 3 3 0 0 4 1 – 0 0 0 0 0 2 5 2 5 1 4 1 3 2 3 6 4
Golgi 1 0 0 0 0 0 – 0 0 0 0 0 1 1 0 1 0 0 0 1 1 3 7 7
Mitochondria 29 11 93 63 14 119 – 113 113 97 133 135 7 1 2 22 15 7 3 2 3 0 1 4
Nucleus 259 342 218 190 247 288 – 247 161 202 177 193 285 320 263 106 70 70 244 307 188 132 193 200
Nuclear Periphery 0 3 3 2 2 3 – 7 5 2 1 4 3 2 1 0 1 0 0 0 0 0 1 0
Nucleolus 39 64 48 61 31 85 – 137 75 99 90 114 59 58 34 11 7 8 16 50 29 32 48 72
Peroxisomes 0 0 0 0 1 0 – 1 0 0 0 0 0 3 3 0 0 0 0 0 0 0 0 0
SpindlePole 1 1 3 0 6 1 – 4 1 2 0 4 3 2 9 2 1 4 2 7 6 4 5 4
Vac/Vac Membrane 2 1 2 3 8 5 – 2 0 3 2 1 2 3 8 17 6 9 7 13 11 9 19 25
Unique Cell Count 279 373 270 233 297 383 381 248 303 276 307 334 397 350 153 104 102 279 395 256 193 292 327
Labelled Cell Count 338 437 394 329 336 526 541 372 433 427 476 382 443 379 188 120 117 279 395 256 193 292 327


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.6 7.2 5.0 5.4 6.5 4.8 – 4.9 4.7 4.7 4.4 4.5 7.1 6.9 6.9 7.4 7.1 7.7 6.9 7.3 7.4
Std Deviation (1e-4) 1.0 1.5 1.7 1.8 3.6 1.7 – 1.7 1.9 1.6 1.4 1.6 1.6 1.6 1.9 1.5 1.6 1.7 2.0 1.6 1.8
Intensity Change (Log2) – – – 0.1 0.38 -0.06 – -0.05 -0.1 -0.11 -0.18 -0.18 0.49 0.45 0.45 0.56 0.49 0.6 0.45 0.54 0.56


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 -0.8 0 – 0.2 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm -1.7 0.3 -1.5 – -1.0 -1.3 -1.1 -1.1 -2.6 0.1 2.8 4.0 2.3 2.8 2.1
Endoplasmic Reticulum 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Mitochondria -1.8 -9.0 0 – -1.3 0 0 0 0 -10.6 -12.5 -11.6 -4.5 -3.8 -5.4
Nucleus 0.2 0.8 -1.7 – -4.4 -4.1 -3.8 -4.3 -4.7 1.5 0 -1.7 -2.7 -2.8 -2.5
Nuclear Periphery 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 2.3 -2.5 0 – 5.1 0 0 0 0 0 -1.1 -2.9 -3.0 -2.7 -2.4
Peroxisomes 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 – 0 0 0 0 0 0 0 1.3 0 0 0
Vacuole 0 0 0 – 0 0 0 0 0 0 0 0 4.9 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 2.6421 1.6406 1.5535 1.8203 0.8465 1.4385 1.6533 1.2351 1.0903 1.5158 1.3429 1.227 2.8628 1.9005 2.0308 2.4227 2.0213 2.215
Actin 0.0218 0.0002 0.021 0.0047 0.0001 0.0053 0.007 0 0.0054 0.0165 0.0024 0.001 0.0899 0 0.0006 0.1445 0 0
Bud 0.0009 0.0001 0.0012 0.0001 0.0002 0.0004 0.0003 0 0.0001 0.0001 0.0003 0.0001 0.0017 0 0.0004 0.0012 0 0.0001
Bud Neck 0.0054 0.0003 0.0005 0.0002 0.0026 0.0017 0.003 0.0001 0.0033 0.0004 0.0068 0.0029 0.0048 0.0002 0.0005 0.0003 0.0003 0.0012
Bud Periphery 0.0026 0.0001 0.0011 0.0001 0.0005 0.0007 0.001 0 0.0003 0.0001 0.0008 0.0002 0.0019 0 0.0004 0.0027 0 0.0001
Bud Site 0.0041 0.0002 0.0178 0.0002 0.0009 0.001 0.002 0 0.0004 0.0003 0.0007 0.0002 0.035 0.0002 0.0039 0.001 0.0001 0.0002
Cell Periphery 0.0004 0 0.0002 0 0.0002 0.0001 0.0003 0 0.0001 0.0001 0.0001 0.0001 0.0004 0 0.0002 0.0001 0 0.0001
Cytoplasm 0.0098 0.0002 0.0185 0.0006 0.0003 0.0035 0.0019 0.0001 0.0009 0.002 0.0015 0.0002 0.0013 0.0001 0.0399 0.0006 0.0004 0.0183
Cytoplasmic Foci 0.0141 0.0002 0.0122 0.005 0.0001 0.0057 0.009 0 0.0018 0.0011 0.0011 0.0003 0.008 0 0.0076 0.0031 0 0.0017
Eisosomes 0.0003 0 0.0003 0.0001 0 0 0.0001 0 0.0001 0.0001 0.0001 0.0001 0.0005 0 0 0.0006 0 0
Endoplasmic Reticulum 0.0124 0 0.0008 0.0001 0.0001 0.0023 0.0014 0 0.0008 0.0014 0.0006 0.0003 0.0049 0 0.0011 0.0019 0 0.0005
Endosome 0.0395 0.0002 0.0177 0.0051 0.0003 0.0198 0.0092 0 0.0029 0.0072 0.0099 0.002 0.0129 0 0.0188 0.0069 0 0.0159
Golgi 0.0117 0.0002 0.0035 0.0128 0.0001 0.013 0.0031 0 0.0022 0.0017 0.0031 0.0005 0.0114 0 0.0035 0.0154 0 0.0001
Lipid Particles 0.0163 0.0013 0.0038 0.088 0.0002 0.008 0.0189 0 0.0028 0.0014 0.0023 0.0047 0.0039 0 0.0129 0.0088 0 0.0001
Mitochondria 0.0357 0.0078 0.0189 0.0104 0.0033 0.0064 0.0054 0 0.0046 0.0022 0.0035 0.0012 0.0046 0.0002 0.0066 0.0044 0.0002 0.0002
None 0.0046 0.0001 0.0004 0.0002 0.0004 0.0003 0.0021 0 0.0003 0.0069 0.0034 0.0002 0.002 0 0.0008 0.0012 0.0002 0.0007
Nuclear Periphery 0.0799 0.0033 0.0015 0.0078 0.0018 0.0177 0.0101 0.0006 0.0028 0.0122 0.0115 0.0083 0.0096 0.0003 0.0026 0.0032 0.0006 0.0023
Nucleolus 0.0217 0.0353 0.0209 0.0062 0.2273 0.099 0.0336 0.0282 0.0174 0.021 0.089 0.0654 0.015 0.0388 0.0298 0.0298 0.0388 0.0843
Nucleus 0.6731 0.9307 0.846 0.8422 0.7486 0.7974 0.8687 0.9617 0.95 0.9182 0.8304 0.9042 0.7858 0.9577 0.8554 0.7681 0.9582 0.8623
Peroxisomes 0.0193 0.0013 0.0041 0.0143 0.0001 0.0097 0.0073 0 0.0017 0.0003 0.0006 0.0008 0.0033 0 0.0073 0.0042 0 0
Punctate Nuclear 0.0152 0.0175 0.0021 0.0013 0.0121 0.0052 0.0116 0.0093 0.0011 0.0044 0.0304 0.007 0.0009 0.0024 0.0021 0.0012 0.0009 0.0011
Vacuole 0.0054 0.0006 0.0055 0.0002 0.0005 0.0019 0.0027 0 0.0006 0.0013 0.001 0.0002 0.0012 0.0001 0.0046 0.0003 0.0002 0.0094
Vacuole Periphery 0.0056 0.0002 0.0018 0.0004 0.0003 0.0009 0.0011 0 0.0004 0.0009 0.0008 0.0003 0.0012 0 0.001 0.0005 0 0.0014

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression – – – – – – – – – –
Translational Efficiency – – – – – – – – – –

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression –
Translational Efficiency –

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
165 1177 358 1224 223 1864 510 1298 388 3041 868 2522

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 761.92 847.17 980.72 980.98 736.85 849.34 985.03 977.44 747.51 848.50 983.25 979.16
Standard Deviation 105.06 132.89 144.74 151.26 111.62 128.28 127.69 148.41 109.58 130.09 135.00 149.81
Intensity Change Log 2 — 0.153012 0.364202 0.364584 — 0.204971 0.418797 0.407637 — 0.178791 0.391301 0.385911

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000535 0.000766 0.001288 0.001225 0.000543 0.000498 0.001125 0.001534 0.000540 0.000602 0.001192 0.001384
Bud Neck 0.007024 0.012230 0.013928 0.029382 0.008390 0.008356 0.023312 0.036359 0.007809 0.009855 0.019442 0.032973
Bud Site 0.000701 0.000961 0.001349 0.005517 0.000759 0.001098 0.002520 0.007870 0.000734 0.001045 0.002037 0.006728
Cell Periphery 0.000069 0.000063 0.000040 0.000116 0.000089 0.000079 0.000063 0.000152 0.000081 0.000073 0.000054 0.000134
Cytoplasm 0.004182 0.002053 0.000345 0.004586 0.002386 0.001826 0.000615 0.006008 0.003150 0.001914 0.000504 0.005318
Cytoplasmic Foci 0.001808 0.000342 0.000028 0.002701 0.000848 0.001016 0.000031 0.003435 0.001256 0.000755 0.000030 0.003079
Eisosomes 0.000031 0.000033 0.000045 0.000033 0.000034 0.000032 0.000047 0.000041 0.000033 0.000032 0.000046 0.000037
Endoplasmic Reticulum 0.002285 0.004136 0.003914 0.004883 0.002940 0.002628 0.003420 0.005878 0.002661 0.003212 0.003624 0.005395
Endosome 0.000177 0.000374 0.000130 0.002049 0.000196 0.000753 0.000252 0.003976 0.000188 0.000606 0.000201 0.003041
Golgi 0.000131 0.000225 0.000153 0.001362 0.000134 0.000791 0.000163 0.002635 0.000133 0.000572 0.000159 0.002017
Lipid Particles 0.000225 0.000789 0.000086 0.000118 0.000437 0.001030 0.000089 0.000414 0.000347 0.000937 0.000088 0.000271
Mitochondria 0.000944 0.004247 0.001150 0.003937 0.001062 0.004201 0.001360 0.006095 0.001012 0.004219 0.001274 0.005048
Mitotic Spindle 0.001459 0.005918 0.000935 0.017336 0.000902 0.009377 0.000933 0.034321 0.001139 0.008038 0.000934 0.026078
None 0.008332 0.002922 0.001958 0.005112 0.005077 0.003277 0.002926 0.008167 0.006462 0.003139 0.002527 0.006684
Nuclear Periphery 0.000209 0.000614 0.000181 0.001935 0.000179 0.000553 0.000200 0.000971 0.000192 0.000577 0.000192 0.001439
Nuclear Periphery Foci 0.000704 0.000462 0.000136 0.000822 0.000551 0.000628 0.000136 0.000815 0.000616 0.000564 0.000136 0.000819
Nucleolus 0.075738 0.061603 0.051738 0.024836 0.063228 0.083963 0.048750 0.019126 0.068548 0.075309 0.049982 0.021897
Nucleus 0.894441* 0.900556* 0.920635* 0.887273* 0.910801* 0.877251* 0.912149* 0.852289* 0.903844* 0.886271* 0.915649* 0.869268*
Peroxisomes 0.000640 0.001004 0.001045 0.000428 0.000842 0.001550 0.000701 0.001756 0.000756 0.001338 0.000843 0.001111
Vacuole 0.000215 0.000408 0.000754 0.004272 0.000432 0.000558 0.001005 0.005388 0.000340 0.000500 0.000902 0.004846
Vacuole Periphery 0.000150 0.000295 0.000161 0.002077 0.000170 0.000535 0.000201 0.002770 0.000161 0.000442 0.000185 0.002434

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -2.10 -7.60 -8.33 -8.42 -0.81 0.60 -8.56 -9.51 -11.11 -2.86 -0.77 -11.44 -12.70 -14.24 -2.75
Bud Neck -2.22 -3.58 -9.73 -12.36 -8.61 0.40 -6.73 -11.08 -15.71 -3.90 -1.15 -7.54 -14.80 -20.42 -7.58
Bud Site -1.49 -4.83 -5.30 -5.00 -4.10 -1.30 -4.57 -8.36 -7.88 -4.51 -1.92 -5.90 -9.35 -8.81 -5.82
Cell Periphery 0.43 1.43 -2.25 -4.42 -6.95 0.30 0.74 -1.05 -3.52 -4.95 0.39 1.14 -1.72 -4.99 -7.42
Cytoplasm 0.81 1.47 0.12 -2.53 -7.24 0.57 1.69 -2.23 -4.01 -6.78 0.99 2.11 -1.07 -4.67 -9.53
Cytoplasmic Foci 0.83 1.08 0.37 -1.25 -2.12 0.14 2.32 -0.39 -0.60 -2.57 0.80 1.81 0.16 -1.18 -3.32
Eisosomes -0.25 -3.98 -2.29 -3.60 2.85 1.03 -2.94 -2.18 -7.45 1.64 0.79 -4.56 -2.86 -7.87 3.27
Endoplasmic Reticulum -4.36 -4.65 -8.28 -3.14 -3.86 0.61 -1.40 -5.55 -11.72 -7.65 -1.27 -3.14 -8.16 -11.22 -8.35
Endosome -2.29 0.48 -2.18 -1.70 -2.23 -2.44 -0.51 -3.64 -2.37 -3.56 -2.98 -0.31 -4.14 -2.76 -4.10
Golgi -1.64 -1.83 -2.89 -2.30 -2.64 -2.17 -0.61 -2.48 -0.90 -2.42 -2.41 -1.35 -3.49 -1.49 -3.34
Lipid Particles -1.63 2.72 1.67 1.92 -3.19 -1.18 2.53 1.17 1.82 -3.51 -1.83 3.05 1.61 2.57 -4.14
Mitochondria -2.78 -2.00 -4.53 0.42 -4.11 -4.21 -2.67 -5.99 -0.40 -5.16 -4.97 -3.31 -7.41 0.11 -6.48
Mitotic Spindle -1.86 -0.49 -6.29 -4.73 -6.02 -2.32 -0.26 -6.36 -5.02 -6.85 -2.99 -0.46 -8.69 -6.70 -9.06
None 2.49 3.14 1.81 -1.97 -4.60 1.26 1.51 -0.61 -2.89 -3.28 2.64 3.26 0.83 -3.46 -4.83
Nuclear Periphery -4.67 -0.40 -5.53 -4.11 -5.50 -1.83 -2.69 -14.21 -4.94 -9.57 -3.16 -2.65 -9.35 -5.91 -8.27
Nuclear Periphery Foci 0.48 1.10 0.35 -0.36 -2.59 0.88 1.22 0.91 0.18 -3.11 0.97 1.48 0.98 0.02 -3.98
Nucleolus 1.90 2.27 6.54 11.25 6.34 -2.48 1.29 7.23 21.96* 8.36 -0.79 2.46 9.67 23.83 10.33
Nucleus -0.61 -1.42 1.42 3.40 4.32 2.48 0.78 3.75 2.24 4.00 1.63 -0.26 3.72 3.46 5.59
Peroxisomes -1.72 -2.57 1.86 3.60 5.69 -1.38 1.24 0.03 1.29 -0.91 -1.84 -0.03 0.66 2.24 0.85
Vacuole -2.18 -6.07 -5.53 -5.29 -4.68 -0.63 -2.62 -6.75 -6.67 -5.74 -1.31 -4.08 -8.69 -8.47 -7.31
Vacuole Periphery -3.08 -1.02 -2.42 -2.07 -2.36 -1.92 -0.52 -2.27 -1.48 -2.22 -2.40 -0.87 -3.28 -2.32 -3.19
ORF
Human Ortholog
Description Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF MRPL9/YGR220C
Localization
Cell Percentages nucleus (94%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-4

YGR219W

YGR219W


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ygr219w-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available