Standard name | |
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Human Ortholog | |
Description | Protein involved in the regulation of cell wall synthesis; proposed to be involved in coordinating cell cycle progression with cell wall integrity |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 64 | 88 | 30 | 30 | 33 | 39 | 26 | 38 | 73 | 42 | 41 | 45 | 90 | 79 | 44 | 71 | 37 | 37 | 59 | 67 | 27 | 63 | 80 | 70 |
Bud | 16 | 8 | 20 | 12 | 6 | 15 | 18 | 27 | 36 | 34 | 20 | 27 | 3 | 10 | 6 | 8 | 5 | 5 | 5 | 12 | 1 | 5 | 2 | 9 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 4 | 3 | 0 | 0 | 0 | 2 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 2 | 1 | 3 | 4 | 2 | 0 | 5 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 5 | 6 | 0 | 1 | 0 | 2 | 0 | 4 | 10 | 7 | 10 | 7 | 5 | 8 | 3 | 8 | 5 | 3 | 1 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 61 | 79 | 36 | 33 | 8 | 8 | 14 | 15 | 27 | 21 | 30 | 20 | 26 | 44 | 52 | 117 | 67 | 49 | 38 | 54 | 19 | 4 | 4 | 8 |
Endoplasmic Reticulum | 51 | 77 | 14 | 10 | 6 | 14 | 8 | 16 | 14 | 11 | 13 | 7 | 37 | 27 | 20 | 15 | 15 | 8 | 42 | 34 | 20 | 13 | 14 | 21 |
Endosome | 3 | 0 | 6 | 3 | 8 | 6 | 19 | 20 | 8 | 8 | 11 | 12 | 5 | 7 | 14 | 21 | 5 | 2 | 22 | 20 | 22 | 19 | 26 | 23 |
Golgi | 90 | 117 | 28 | 31 | 82 | 34 | 38 | 37 | 41 | 28 | 19 | 32 | 282 | 300 | 275 | 209 | 154 | 122 | 118 | 124 | 46 | 191 | 193 | 225 |
Mitochondria | 69 | 46 | 52 | 61 | 129 | 125 | 164 | 199 | 126 | 196 | 92 | 182 | 193 | 212 | 222 | 31 | 23 | 16 | 4 | 8 | 5 | 5 | 2 | 12 |
Nucleus | 0 | 1 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 1 | 3 | 1 | 2 | 0 | 2 | 3 | 0 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
Nucleolus | 4 | 0 | 4 | 4 | 4 | 9 | 22 | 15 | 3 | 19 | 1 | 12 | 5 | 7 | 16 | 6 | 6 | 4 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 5 | 2 | 2 | 5 | 3 | 2 | 0 | 2 | 2 | 2 | 5 | 1 | 0 | 0 | 1 | 1 | 1 | 2 | 5 | 5 |
SpindlePole | 0 | 0 | 0 | 1 | 0 | 1 | 2 | 6 | 3 | 3 | 1 | 4 | 1 | 1 | 0 | 9 | 4 | 0 | 1 | 3 | 0 | 3 | 2 | 0 |
Vac/Vac Membrane | 0 | 4 | 2 | 2 | 6 | 2 | 11 | 16 | 4 | 1 | 4 | 5 | 7 | 10 | 14 | 24 | 6 | 6 | 1 | 16 | 5 | 5 | 2 | 8 |
Unique Cell Count | 251 | 291 | 124 | 126 | 188 | 158 | 234 | 290 | 219 | 265 | 165 | 247 | 404 | 470 | 488 | 413 | 238 | 197 | 304 | 355 | 155 | 317 | 341 | 395 |
Labelled Cell Count | 363 | 426 | 192 | 188 | 289 | 262 | 327 | 403 | 352 | 376 | 245 | 361 | 657 | 708 | 672 | 523 | 328 | 252 | 304 | 355 | 155 | 317 | 341 | 395 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 19.1 | 20.7 | 13.1 | 10.9 | 12.9 | 11.7 | 10.0 | 10.1 | 11.0 | 10.1 | 9.9 | 10.3 | 20.5 | 19.8 | 19.3 | 18.4 | 22.9 | 23.4 | 18.7 | 18.0 | 19.0 |
Std Deviation (1e-4) | 3.5 | 4.2 | 2.3 | 3.1 | 2.5 | 2.5 | 2.4 | 2.2 | 2.1 | 2.0 | 2.0 | 1.9 | 4.0 | 4.5 | 5.3 | 5.0 | 6.5 | 6.4 | 3.9 | 3.8 | 3.9 |
Intensity Change (Log2) | – | – | – | -0.27 | -0.03 | -0.17 | -0.39 | -0.38 | -0.26 | -0.38 | -0.41 | -0.36 | 0.64 | 0.59 | 0.55 | 0.48 | 0.8 | 0.83 | 0.51 | 0.45 | 0.54 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | -0.1 | -1.4 | 0.1 | -3.2 | -2.8 | 1.8 | -2.0 | 0.1 | -1.4 | -0.4 | -1.9 | -4.6 | -1.7 | -2.0 | -1.2 |
Bud | -1.6 | -4.0 | -1.7 | -2.5 | -2.0 | 0.1 | -0.9 | -1.0 | -1.4 | -7.3 | -6.3 | -7.3 | -6.2 | -5.0 | -4.4 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | -0.5 | -6.2 | -5.5 | -6.0 | -6.8 | -3.8 | -5.5 | -2.2 | -5.3 | -6.8 | -5.7 | -5.2 | -0.2 | -0.2 | -0.8 |
Endoplasmic Reticulum | -0.9 | -2.9 | -0.7 | -3.0 | -2.1 | -1.6 | -2.7 | -1.0 | -3.3 | -0.7 | -2.2 | -3.1 | -3.3 | -1.7 | -2.5 |
Endosome | 0 | -0.2 | -0.4 | 1.2 | 0.8 | -0.5 | 0 | 0.7 | 0 | 0 | 0 | 0 | 0.1 | 0 | 0 |
Golgi | 0.4 | 3.8 | -0.2 | -1.5 | -2.5 | -0.9 | -3.1 | -2.5 | -2.4 | 9.3 | 8.2 | 6.7 | 5.5 | 7.6 | 6.9 |
Mitochondria | 1.0 | 4.7 | 6.4 | 5.2 | 5.1 | 2.8 | 6.1 | 2.3 | 6.0 | 1.1 | 0.6 | 0.7 | -9.3 | -7.2 | -7.2 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 1.0 | 2.1 | 0.9 | 0 | 1.5 | 0 | 0.7 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 0 | 1.8 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1.9 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 18.7112 | 20.953 | 21.3864 | 21.4725 | 20.5385 | 22.0043 | 12.5473 | 17.8306 | 16.7065 | 16.0336 | 16.6629 | 15.4505 | 16.843 | 18.092 | 19.7 | 18.0106 | 17.3675 | 18.1645 |
Actin | 0.1264 | 0.0442 | 0.0585 | 0.0329 | 0.0391 | 0.042 | 0.1751 | 0.1725 | 0.1409 | 0.0813 | 0.0996 | 0.0989 | 0.2382 | 0.1358 | 0.0772 | 0.0172 | 0.0134 | 0.115 |
Bud | 0.0012 | 0.0033 | 0.0084 | 0.0048 | 0.0014 | 0.0002 | 0.0045 | 0.0015 | 0.0108 | 0.0021 | 0.0004 | 0.0003 | 0.0072 | 0.0042 | 0.02 | 0.0018 | 0.0124 | 0.0007 |
Bud Neck | 0.0045 | 0.0013 | 0.0023 | 0.0022 | 0.0026 | 0.0027 | 0.004 | 0.0006 | 0.0016 | 0.0012 | 0.0007 | 0.0011 | 0.002 | 0.0009 | 0.0012 | 0.0009 | 0.0011 | 0.0011 |
Bud Periphery | 0.0015 | 0.0019 | 0.0053 | 0.0052 | 0.0024 | 0.0002 | 0.005 | 0.0006 | 0.0094 | 0.0007 | 0.0003 | 0.0003 | 0.0023 | 0.0015 | 0.0122 | 0.0009 | 0.0156 | 0.0007 |
Bud Site | 0.0027 | 0.0169 | 0.01 | 0.0023 | 0.0016 | 0.0002 | 0.0084 | 0.0018 | 0.0041 | 0.0016 | 0.0004 | 0.0002 | 0.0444 | 0.0054 | 0.0036 | 0.0004 | 0.061 | 0.0006 |
Cell Periphery | 0.001 | 0.001 | 0.0008 | 0.0011 | 0.0006 | 0.0002 | 0.0005 | 0.0007 | 0.0008 | 0.0002 | 0.0001 | 0.0001 | 0.0004 | 0.0002 | 0.0006 | 0.0007 | 0.0018 | 0.0001 |
Cytoplasm | 0.0303 | 0.0389 | 0.0891 | 0.1113 | 0.0319 | 0.0036 | 0.0172 | 0.0208 | 0.0614 | 0.0743 | 0.0385 | 0.0038 | 0.0282 | 0.0563 | 0.0814 | 0.2953 | 0.1007 | 0.0047 |
Cytoplasmic Foci | 0.0726 | 0.1304 | 0.0803 | 0.1002 | 0.1027 | 0.0374 | 0.0683 | 0.0546 | 0.0869 | 0.1108 | 0.081 | 0.0326 | 0.0706 | 0.1338 | 0.0993 | 0.1092 | 0.0649 | 0.0345 |
Eisosomes | 0.0006 | 0.0003 | 0.0001 | 0.0001 | 0.0002 | 0.0001 | 0.001 | 0.0001 | 0.0004 | 0 | 0 | 0.0002 | 0.0003 | 0.0001 | 0.0003 | 0 | 0.0001 | 0.0002 |
Endoplasmic Reticulum | 0.008 | 0.0015 | 0.003 | 0.0045 | 0.0081 | 0.0011 | 0.0049 | 0.0004 | 0.0074 | 0.002 | 0.0059 | 0.0021 | 0.0021 | 0.0013 | 0.0021 | 0.006 | 0.002 | 0.0007 |
Endosome | 0.1905 | 0.2421 | 0.2792 | 0.3305 | 0.3153 | 0.1352 | 0.1597 | 0.158 | 0.1991 | 0.3403 | 0.221 | 0.0931 | 0.1244 | 0.2368 | 0.1921 | 0.2088 | 0.2023 | 0.0874 |
Golgi | 0.4406 | 0.4309 | 0.3898 | 0.3292 | 0.4341 | 0.7505 | 0.4122 | 0.5523 | 0.4338 | 0.3492 | 0.5329 | 0.7274 | 0.453 | 0.402 | 0.4804 | 0.2673 | 0.5102 | 0.7069 |
Lipid Particles | 0.0404 | 0.0279 | 0.0238 | 0.0243 | 0.0222 | 0.0085 | 0.0413 | 0.0017 | 0.0165 | 0.016 | 0.0052 | 0.0134 | 0.0058 | 0.007 | 0.0053 | 0.0714 | 0.0007 | 0.0043 |
Mitochondria | 0.029 | 0.0148 | 0.0162 | 0.0068 | 0.0195 | 0.0121 | 0.0253 | 0.019 | 0.006 | 0.0035 | 0.0026 | 0.0086 | 0.0036 | 0.0061 | 0.0103 | 0.0015 | 0.0051 | 0.0385 |
None | 0.0097 | 0.0003 | 0.0009 | 0.0003 | 0.0003 | 0 | 0.0288 | 0.0001 | 0.001 | 0.0001 | 0.0001 | 0.0009 | 0.0005 | 0.0001 | 0.0002 | 0.0002 | 0.0007 | 0.0001 |
Nuclear Periphery | 0.007 | 0.001 | 0.0018 | 0.001 | 0.0026 | 0.0001 | 0.0026 | 0.0001 | 0.0016 | 0.0005 | 0.0008 | 0.0059 | 0.0003 | 0.0003 | 0.0006 | 0.0023 | 0.0006 | 0.0001 |
Nucleolus | 0.0012 | 0.0007 | 0.001 | 0.0002 | 0.0001 | 0 | 0.0035 | 0.0001 | 0.0001 | 0 | 0 | 0.0002 | 0.0001 | 0 | 0.0001 | 0.0001 | 0.0001 | 0.0001 |
Nucleus | 0.0024 | 0.0009 | 0.0012 | 0.0005 | 0.0005 | 0 | 0.0033 | 0.0002 | 0.0004 | 0.0001 | 0.0001 | 0.0005 | 0.0003 | 0.0001 | 0.0003 | 0.0012 | 0.0009 | 0.0001 |
Peroxisomes | 0.0092 | 0.0047 | 0.0043 | 0.0019 | 0.0034 | 0.0013 | 0.0095 | 0.0037 | 0.0062 | 0.008 | 0.001 | 0.005 | 0.0139 | 0.0034 | 0.0029 | 0.0009 | 0.0015 | 0.0021 |
Punctate Nuclear | 0.0045 | 0.0025 | 0.0016 | 0.0006 | 0.0006 | 0.0001 | 0.0158 | 0.0001 | 0.0008 | 0.0002 | 0.0001 | 0.0034 | 0.0006 | 0.0002 | 0.0003 | 0.0003 | 0.0003 | 0.0002 |
Vacuole | 0.0119 | 0.024 | 0.0158 | 0.0324 | 0.0063 | 0.0017 | 0.006 | 0.0087 | 0.0062 | 0.0047 | 0.0065 | 0.0006 | 0.001 | 0.0025 | 0.0062 | 0.0103 | 0.003 | 0.0007 |
Vacuole Periphery | 0.0048 | 0.0106 | 0.0067 | 0.0076 | 0.0044 | 0.0026 | 0.0031 | 0.0025 | 0.0046 | 0.0031 | 0.0027 | 0.0013 | 0.0008 | 0.0016 | 0.0034 | 0.0033 | 0.0016 | 0.0011 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 87.2256 | 59.1306 | 64.6107 | 70.0383 | 73.5097 | 74.8272 | 62.9076 | 76.2699 | 63.5376 | 71.9949 |
Translational Efficiency | 1.0698 | 1.2551 | 1.0707 | 1.0382 | 0.9657 | 0.9824 | 0.8974 | 0.7355 | 0.9924 | 0.9365 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✔ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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