Standard name
Human Ortholog
Description Evolutionarily conserved 19S regulatory particle assembly-chaperone; proteasome-interacting protein involved in the assembly of the base subcomplex of the 19S proteasomal regulatory particle (RP); ortholog of human oncoprotein gankyrin, also known as p28, which interacts with the Rb tumor suppressor and CDK4/6
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.88 1.0 0.99 0.98 0.95 0.93 0.84 – 0.77 0.74 0.72 0.68 0.99 1.0 1.0 0.94 0.96 0.99 0.89 0.96 0.97 0.94 0.94 0.9
Endoplasmic Reticulum 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.1 0 – 0.1 0.08 0.17 0.16 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0.14 – 0.18 0.18 0.14 0.17 0 0 0 0.06 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 1 1 1 – 0 1 2 1 0 0 0 0 0 0 1 0 0 0 2 6
Bud 0 0 0 0 0 4 1 – 0 2 2 12 0 0 0 0 0 0 2 0 0 0 5 8
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 2 1 4 9 16 4 23 – 11 12 20 30 2 7 7 8 5 5 0 0 0 0 0 0
Cytoplasm 192 137 225 298 447 388 662 – 358 417 469 491 295 447 530 225 165 172 274 138 224 225 551 471
Endoplasmic Reticulum 3 0 0 2 3 1 6 – 6 8 4 10 1 0 0 10 7 7 4 0 0 4 5 7
Endosome 1 0 1 0 1 0 0 – 0 1 1 2 0 0 0 1 0 0 4 0 1 0 2 5
Golgi 0 0 0 0 0 0 2 – 0 0 0 0 1 0 1 0 0 0 0 0 1 2 4 2
Mitochondria 5 0 0 4 3 42 18 – 48 45 111 118 0 0 0 1 0 0 3 1 1 2 1 5
Nucleus 7 0 0 1 3 3 11 – 13 15 19 15 0 0 0 8 2 0 0 0 0 0 0 0
Nuclear Periphery 8 0 0 1 0 0 0 – 0 5 2 7 0 0 0 0 0 0 0 0 0 1 0 1
Nucleolus 2 0 0 0 0 0 0 – 0 0 0 2 0 0 1 0 0 0 0 0 0 0 1 0
Peroxisomes 1 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 1 – 0 0 0 0 0 0 0 0 0 0 3 0 0 0 4 4
Vac/Vac Membrane 7 0 1 1 18 8 114 – 84 104 91 122 2 2 1 14 7 2 6 0 0 2 2 3
Unique Cell Count 219 137 228 303 473 418 788 467 566 650 727 298 449 532 240 171 173 309 144 231 241 589 527
Labelled Cell Count 228 138 231 316 492 451 839 520 610 721 810 301 456 540 267 186 186 309 144 231 241 589 527


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 7.2 6.2 7.1 6.7 8.2 6.0 7.8 – 7.3 7.5 7.1 7.4 8.1 8.9 9.1 13.6 13.4 14.2 6.7 7.3 7.7
Std Deviation (1e-4) 8.1 0.9 1.0 1.4 1.6 1.2 1.6 – 2.0 1.6 1.6 1.7 1.4 1.6 1.7 3.3 2.5 3.0 1.5 1.8 1.6
Intensity Change (Log2) – – – -0.08 0.21 -0.26 0.13 – 0.04 0.07 -0.01 0.04 0.19 0.32 0.35 0.93 0.91 0.99 -0.08 0.03 0.12


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0.9 1.2 0 1.0 – 0 0 1.1 1.7 0 0 0 1.1 0 0
Cytoplasm -0.3 -2.6 -3.2 -5.9 – -7.4 -8.1 -8.5 -9.5 0.3 1.3 1.5 -2.8 -1.5 0.7
Endoplasmic Reticulum 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 4.9 0 – 5.0 4.4 6.7 6.5 0 0 0 0 0 0
Nucleus 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Vacuole 0 2.6 0 5.9 – 6.6 6.8 5.8 6.4 0 0 0 3.3 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 2.0006 2.8076 2.4327 1.7212 2.8588 2.6261 3.5866 4.9137 4.0262 3.6534 2.9519 4.1874 5.4072 7.1083 6.0604 6.2827 5.7767 6.1691
Actin 0.0381 0.0003 0.008 0.0002 0.0001 0.0099 0.0456 0.0009 0.0094 0.0012 0.0124 0.0059 0.0069 0.0004 0.0009 0.0042 0.0016 0.004
Bud 0.0009 0.0002 0.0003 0.0002 0.0001 0.0003 0.005 0.0227 0.0109 0.006 0.0011 0.0088 0.0002 0.0001 0.0002 0.0005 0.0025 0.0001
Bud Neck 0.0013 0.0003 0.0004 0.0003 0.0003 0.0027 0.0076 0.0002 0.0006 0.0009 0.0008 0.0012 0.0007 0.0001 0.0006 0.0011 0.0012 0.0008
Bud Periphery 0.0012 0.0001 0.0003 0 0 0.0002 0.0035 0.0034 0.0038 0.0023 0.0008 0.0039 0.0001 0 0.0001 0.0004 0.0019 0.0001
Bud Site 0.0023 0.001 0.0029 0.0001 0 0.0002 0.009 0.0089 0.0048 0.0013 0.0014 0.0004 0.0004 0.0013 0.0004 0.0016 0.0019 0.0001
Cell Periphery 0.0002 0.0001 0.0002 0 0 0 0.0005 0.0002 0.0003 0.0001 0.0001 0.0001 0.0001 0.0001 0 0.0001 0.0001 0
Cytoplasm 0.6931 0.8826 0.8234 0.9495 0.8945 0.8038 0.6598 0.7811 0.8247 0.7101 0.7148 0.8913 0.8879 0.9199 0.9228 0.9013 0.8867 0.9346
Cytoplasmic Foci 0.0256 0.0042 0.007 0.009 0.0139 0.0069 0.0323 0.0031 0.0096 0.0522 0.0685 0.0071 0.0123 0.0042 0.0077 0.0099 0.0053 0.0056
Eisosomes 0.0003 0 0.0001 0 0 0 0.0005 0.0001 0.0001 0 0.0001 0 0 0 0 0 0 0
Endoplasmic Reticulum 0.0079 0.0031 0.003 0.0019 0.0021 0.0065 0.0053 0.0013 0.0036 0.0014 0.0044 0.0021 0.0035 0.0011 0.0021 0.0025 0.0022 0.002
Endosome 0.037 0.0046 0.0075 0.0157 0.0067 0.0156 0.0447 0.001 0.0149 0.0379 0.0321 0.0044 0.0042 0.0017 0.0029 0.0069 0.0013 0.0036
Golgi 0.0157 0.0002 0.0043 0.0003 0.0001 0.0078 0.0083 0.0002 0.0088 0.0225 0.0149 0.0009 0.0008 0.0002 0.0004 0.0011 0.0002 0.0005
Lipid Particles 0.0184 0.0001 0.0042 0 0.0003 0.0017 0.0181 0.0013 0.0047 0.0065 0.038 0.001 0.0008 0 0.0005 0.0002 0.0001 0.0002
Mitochondria 0.0129 0.0001 0.0096 0.0002 0.0001 0.0029 0.0057 0.0026 0.0103 0.0062 0.0425 0.0049 0.0003 0.0001 0.001 0.0024 0.0002 0.0003
None 0.0468 0.0214 0.0231 0.0107 0.0099 0.0093 0.0443 0.0298 0.0279 0.0297 0.0282 0.0134 0.0703 0.039 0.0355 0.0309 0.0568 0.0271
Nuclear Periphery 0.0106 0.0025 0.0115 0.0015 0.0018 0.0092 0.0161 0.0024 0.0033 0.0024 0.0017 0.0031 0.0011 0.0007 0.0009 0.0021 0.0014 0.001
Nucleolus 0.0004 0.0002 0.0001 0 0.0001 0.0008 0.0017 0.0022 0.0011 0.0002 0.0002 0.0001 0.0001 0 0.0001 0.0002 0.0002 0
Nucleus 0.0696 0.0752 0.0812 0.0075 0.0675 0.1129 0.0448 0.1352 0.0478 0.0953 0.0035 0.0473 0.0022 0.0303 0.0192 0.0304 0.0343 0.0172
Peroxisomes 0.0046 0 0.0103 0.0001 0.0001 0.0006 0.0213 0.0002 0.0031 0.0157 0.0231 0.0004 0.0021 0.0001 0.0032 0.001 0.0001 0.0012
Punctate Nuclear 0.0075 0.0014 0.0005 0.0006 0.0016 0.0066 0.0107 0.0017 0.0078 0.0056 0.0062 0.0022 0.0053 0.0003 0.0008 0.0012 0.0011 0.001
Vacuole 0.0042 0.002 0.0015 0.0019 0.0006 0.0014 0.012 0.0011 0.0015 0.0016 0.0042 0.0009 0.0007 0.0003 0.0007 0.0014 0.0008 0.0004
Vacuole Periphery 0.0015 0.0002 0.0006 0.0005 0.0002 0.0006 0.0032 0.0004 0.0008 0.0008 0.0009 0.0003 0.0001 0 0.0001 0.0005 0.0001 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 56.2142 48.8163 52.2049 73.5908 63.5133 51.3674 68.9562 76.4248 81.3206 70.7024
Translational Efficiency 1.8768 1.7001 1.8386 1.6164 1.397 2.263 1.5557 1.8186 1.5453 1.4688

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1866 1046 304 2071 920 2309 1463 1559 2786 3355 1767 3630

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 752.44 865.45 1211.98 1121.84 750.38 888.19 1046.68 1134.96 751.76 881.10 1075.12 1127.47
Standard Deviation 94.03 115.00 150.14 186.91 90.15 112.91 160.17 178.75 92.77 114.05 170.33 183.56
Intensity Change Log 2 — 0.201874 0.687717 0.576218 — 0.243247 0.480127 0.596948 — 0.222680 0.587795 0.586606

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000518 0.000254 0.000804 0.001230 0.000098 0.000254 0.000188 0.001554 0.000379 0.000254 0.000294 0.001369
Bud Neck 0.010443 0.006660 0.002105 0.003354 0.004044 0.007474 0.000814 0.001323 0.008330 0.007220 0.001036 0.002481
Bud Site 0.007038 0.014365 0.004732 0.029038 0.003703 0.012631 0.004808 0.021335 0.005936 0.013171 0.004795 0.025730
Cell Periphery 0.000081 0.000100 0.000202 0.000108 0.000092 0.000052 0.000075 0.000083 0.000084 0.000067 0.000096 0.000098
Cytoplasm 0.584703* 0.669890* 0.465553* 0.736074* 0.648275* 0.757262* 0.768673* 0.848453* 0.605696* 0.730022* 0.716523* 0.784338*
Cytoplasmic Foci 0.205323* 0.149553 0.012702 0.025748 0.165930 0.082053 0.013036 0.023517 0.192314* 0.103098 0.012979 0.024790
Eisosomes 0.000084 0.000039 0.000033 0.000011 0.000061 0.000025 0.000012 0.000012 0.000077 0.000029 0.000016 0.000012
Endoplasmic Reticulum 0.000998 0.001364 0.003931 0.000553 0.001342 0.000268 0.000780 0.000167 0.001112 0.000610 0.001322 0.000387
Endosome 0.011383 0.011539 0.004726 0.007351 0.010512 0.004727 0.001108 0.001922 0.011095 0.006851 0.001730 0.005019
Golgi 0.003317 0.002565 0.000249 0.007561 0.001311 0.002327 0.000091 0.001226 0.002655 0.002401 0.000118 0.004840
Lipid Particles 0.004129 0.002205 0.002078 0.001098 0.003316 0.001184 0.000443 0.000600 0.003861 0.001502 0.000724 0.000884
Mitochondria 0.003790 0.001212 0.000301 0.001597 0.003733 0.001441 0.000114 0.000437 0.003771 0.001370 0.000146 0.001099
Mitotic Spindle 0.002619 0.002778 0.002497 0.063146 0.002089 0.006756 0.003714 0.009264 0.002444 0.005516 0.003505 0.040005
None 0.001708 0.002360 0.003974 0.002073 0.002655 0.002318 0.008918 0.002890 0.002021 0.002331 0.008067 0.002424
Nuclear Periphery 0.000218 0.000173 0.001247 0.000257 0.000467 0.000105 0.000315 0.000077 0.000300 0.000126 0.000475 0.000180
Nuclear Periphery Foci 0.000385 0.001262 0.008091 0.001308 0.001136 0.000295 0.002691 0.001076 0.000633 0.000597 0.003620 0.001209
Nucleolus 0.000531 0.000544 0.000200 0.000420 0.000673 0.000324 0.000120 0.000093 0.000578 0.000393 0.000134 0.000279
Nucleus 0.087159 0.055632 0.336001* 0.034726 0.089523 0.064551 0.139091* 0.034675 0.087939 0.061770 0.172968* 0.034704
Peroxisomes 0.002690 0.003325 0.000191 0.001246 0.001407 0.002145 0.000104 0.000591 0.002266 0.002513 0.000119 0.000965
Vacuole 0.070861 0.072441 0.150167 0.078479 0.058960 0.053155 0.054827 0.049981 0.066931 0.059168 0.071230 0.066240
Vacuole Periphery 0.002023 0.001741 0.000216 0.004620 0.000672 0.000653 0.000078 0.000725 0.001577 0.000992 0.000102 0.002947

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 0.96 -1.33 -0.19 -2.30 1.97 -1.92 -7.41 -2.03 -1.59 -1.65 0.66 0.13 -1.27 -2.36 -1.91
Bud Neck 4.19 9.94 10.88 7.14 2.27 -3.64 4.11 4.13 8.85 0.04 1.56 12.41 11.81 10.23 -1.95
Bud Site -5.13 0.64 -5.48 -0.33 -5.83 -6.84 -1.94 -7.01 -1.00 -5.54 -7.04 0.48 -8.25 -1.20 -7.93
Cell Periphery -2.89 -8.16 0.92 2.58 7.78 8.11 1.85 3.06 -2.84 1.66 5.35 -4.01 2.22 -0.91 4.73
Cytoplasm -7.87 13.59 -13.87 -3.70 -21.30 -11.35 -4.23 -14.41 -4.64 -12.23 -18.77 -3.90 -20.83 -2.05 -13.81
Cytoplasmic Foci 8.11 41.69 39.44 23.42 -7.02 13.62 26.90 24.40 19.50 -8.35 20.50 48.94 46.04 30.44 -9.16
Eisosomes 10.70 9.20 20.50 13.06 12.09 10.10 12.77 13.45 7.20 3.08 16.50 19.95 24.07 14.77 9.67
Endoplasmic Reticulum -1.00 -8.58 5.52 3.00 10.85 3.15 1.07 3.47 2.72 10.54 2.77 -2.65 6.23 3.21 13.51
Endosome 0.10 7.04 9.07 7.12 2.26 4.12 6.89 6.80 8.89 -0.26 5.28 13.09 11.78 9.24 -1.69
Golgi 1.75 9.05 0.99 -0.19 -3.77 -1.74 3.39 1.62 3.26 -1.97 0.69 9.92 2.01 1.23 -4.36
Lipid Particles 2.45 3.76 6.29 1.76 3.95 4.14 6.05 5.78 1.40 -0.69 5.33 8.35 8.23 2.01 0.55
Mitochondria 3.04 4.71 4.21 1.11 -2.73 1.26 1.99 1.93 4.23 -1.26 3.01 4.67 4.25 3.55 -4.21
Mitotic Spindle -0.05 -1.10 -9.33 -9.44 -8.89 -2.89 -1.33 -2.41 0.27 -1.61 -2.72 -1.43 -9.42 -7.47 -8.83
None -2.25 -3.16 -3.11 -0.10 1.57 0.77 -6.98 0.12 -1.25 8.84 -1.26 -9.92 -2.08 -0.87 9.37
Nuclear Periphery 1.15 -15.48 -4.70 -5.92 14.11 1.90 -0.44 1.67 -4.16 17.10 2.64 -6.85 0.47 -9.07 16.26
Nuclear Periphery Foci -6.54 -9.51 -3.91 1.26 8.57 7.30 -9.48 -1.13 -10.72 9.23 0.30 -15.41 -4.73 -4.71 12.27
Nucleolus -0.18 6.78 2.85 2.31 -0.94 2.59 4.19 4.54 5.49 2.18 2.87 8.14 5.70 3.34 -1.33
Nucleus 8.77 -15.44 12.78 2.02 18.13 5.69 -12.69 9.70 6.29 25.74 9.90 -20.47 16.21 6.54 30.51
Peroxisomes -1.10 10.79 7.73 5.45 -2.54 -1.87 4.96 3.66 5.39 -3.86 -0.76 12.05 8.86 7.22 -4.13
Vacuole -1.88 -12.96 -17.97 -14.32 6.27 1.20 -9.09 -9.23 -11.44 0.58 1.72 -13.47 -19.52 -20.95 -0.74
Vacuole Periphery 0.52 3.37 0.57 0.09 -3.27 0.16 5.47 1.44 1.16 -1.39 1.56 4.14 1.12 -0.38 -3.79
Standard name
Human Ortholog
Description Evolutionarily conserved 19S regulatory particle assembly-chaperone; proteasome-interacting protein involved in the assembly of the base subcomplex of the 19S proteasomal regulatory particle (RP); ortholog of human oncoprotein gankyrin, also known as p28, which interacts with the Rb tumor suppressor and CDK4/6
Localization
Cell Percentages cytoplasm (87%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Nas6

Nas6


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Nas6-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available