ORF
Human Ortholog
Description Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0.08 0.05 0.05 0 0 0 0 0 0 0.05 0 0.05 0 0 0.06 0.07 0.08 0 0 0 0.06 0.06 0.06 0.07
Cytoplasm 0.99 0.98 0.99 0.99 0.99 0.96 0.98 0.94 0.9 0.88 0.73 0.84 0.99 0.99 0.99 0.95 0.95 0.96 0.9 0.88 0.75 0.69 0.67 0.63
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.09 0.06 0.08 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0.08 0.06 0.22 0.27 0.47 0.2 0 0 0 0.05 0 0 0 0 0.07 0.08 0.11 0.06
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.05 0.07
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 2 0 0 1 1 1 2 0 0 0 1 0 0 0 1 2 2 0 4 4 1 4 8
Bud 3 6 0 0 0 2 1 2 0 1 4 1 1 0 0 0 1 0 0 0 3 0 3 4
Bud Neck 0 8 8 2 6 0 4 5 0 0 1 1 0 0 0 8 6 3 1 4 2 3 8 10
Bud Site 0 0 0 0 0 0 0 1 0 0 0 1 0 0 0 0 0 0 – – – – – –
Cell Periphery 7 21 11 4 9 9 7 7 2 4 3 9 8 10 17 33 27 14 2 11 13 4 15 15
Cytoplasm 84 405 230 133 203 215 235 235 97 71 90 145 217 261 274 457 311 329 89 350 157 58 184 141
Endoplasmic Reticulum 0 2 1 0 0 0 0 1 0 0 0 2 0 0 1 45 18 27 1 1 0 1 2 4
Endosome 0 0 0 0 0 0 1 1 0 0 0 0 0 0 0 4 0 3 1 2 2 0 2 2
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 3 2 0 0 0 0 0 1 0
Mitochondria 2 2 0 2 1 10 19 14 24 22 58 35 2 1 0 22 9 4 0 7 15 6 30 12
Nucleus 0 0 2 1 0 2 0 2 1 1 1 0 1 0 1 2 3 0 0 0 1 0 0 0
Nuclear Periphery 0 2 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 1 0 0 0 0 1 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 1 0 0 0 0 0 0
SpindlePole 0 1 0 1 1 0 0 6 1 0 0 3 0 1 0 2 4 1 0 0 0 0 1 1
Vac/Vac Membrane 0 0 1 0 0 0 0 1 0 0 0 0 0 1 0 6 3 3 1 3 2 3 12 15
Unique Cell Count 85 412 233 135 205 223 240 251 108 81 124 172 220 264 276 481 326 342 100 398 212 84 275 225
Labelled Cell Count 96 449 253 143 221 239 268 277 125 99 157 199 229 274 294 583 386 389 100 398 212 84 275 225


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.3 3.9 4.8 4.7 5.2 4.3 4.4 4.8 3.9 3.9 3.9 4.3 4.5 4.5 4.5 6.3 6.8 5.8 4.4 3.9 4.5
Std Deviation (1e-4) 0.7 0.4 1.2 1.4 1.7 1.5 1.3 1.5 1.3 1.5 1.3 1.0 1.0 1.3 1.3 1.2 2.0 1.0 2.0 1.1 1.8
Intensity Change (Log2) – – – -0.04 0.11 -0.16 -0.12 0.02 -0.28 -0.29 -0.3 -0.15 -0.1 -0.09 -0.08 0.4 0.51 0.28 -0.13 -0.28 -0.08


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 0.5704 0.5301 0.322 1.0808 0.2619 0.2355 1.1951 1.1404 1.0611 1.3587 1.0678 1.0272 0.5247 0.9406 0.6081 0.5675 0.8983 0.7916
Actin 0.0378 0.0095 0.0198 0.0501 0.0406 0.0089 0.0351 0.0121 0.024 0.0401 0.0299 0.0094 0.0461 0.0061 0.004 0.0054 0.0006 0.0068
Bud 0.0024 0.0021 0.0006 0.0132 0.0068 0.0033 0.0034 0.0116 0.0047 0.0083 0.0012 0.0058 0.0024 0.0049 0.0044 0.0029 0.0129 0.0006
Bud Neck 0.0038 0.0023 0.0034 0.0073 0.0037 0.0053 0.0089 0.0016 0.0113 0.0138 0.001 0.0044 0.003 0.0016 0.001 0.0115 0.0137 0.0027
Bud Periphery 0.0061 0.0044 0.0015 0.0291 0.0274 0.0126 0.0112 0.0166 0.0115 0.0242 0.0036 0.0305 0.0057 0.0071 0.0088 0.008 0.005 0.0022
Bud Site 0.0047 0.0368 0.0192 0.0538 0.015 0.0011 0.0184 0.056 0.0355 0.0134 0.0013 0.0028 0.0189 0.0485 0.0182 0.0052 0.0177 0.0004
Cell Periphery 0.004 0.0062 0.0059 0.0017 0.0052 0.0023 0.0053 0.0115 0.0087 0.0128 0.0034 0.0044 0.0055 0.0043 0.0037 0.0037 0.0012 0.0012
Cytoplasm 0.3745 0.4902 0.4502 0.2984 0.5161 0.481 0.3794 0.5535 0.5069 0.4096 0.395 0.5848 0.3724 0.5109 0.4611 0.5035 0.452 0.5753
Cytoplasmic Foci 0.0342 0.0071 0.013 0.0162 0.0306 0.0173 0.0283 0.0029 0.0127 0.0127 0.0124 0.0069 0.0243 0.0044 0.0096 0.0108 0.0072 0.0209
Eisosomes 0.004 0.0006 0.0011 0.0008 0.0005 0.0003 0.0008 0.0007 0.0007 0.0008 0.0006 0.0002 0.001 0.0004 0.0004 0.0002 0.0001 0.0002
Endoplasmic Reticulum 0.0071 0.0034 0.0037 0.0037 0.007 0.0027 0.0085 0.0031 0.0037 0.007 0.0107 0.0025 0.0067 0.0016 0.0034 0.0017 0.0009 0.0017
Endosome 0.0282 0.0014 0.0079 0.0146 0.0196 0.0076 0.0403 0.002 0.0187 0.0266 0.0516 0.0059 0.0197 0.0006 0.0068 0.0148 0.0524 0.0098
Golgi 0.0069 0.0007 0.0035 0.0051 0.0072 0.0034 0.0088 0.0007 0.0099 0.0104 0.0653 0.0053 0.0087 0.0007 0.0025 0.0201 0.0105 0.0063
Lipid Particles 0.0114 0.0008 0.0043 0.006 0.0114 0.0042 0.0133 0.0007 0.0092 0.0104 0.0402 0.0021 0.0273 0.0004 0.0059 0.0012 0.011 0.004
Mitochondria 0.0049 0.0003 0.0024 0.0035 0.0036 0.0029 0.0127 0.0008 0.0072 0.0036 0.0157 0.0109 0.0139 0.0003 0.0172 0.0023 0.0405 0.0078
None 0.3927 0.4286 0.4462 0.4179 0.2178 0.4326 0.3693 0.3184 0.3065 0.387 0.3261 0.3101 0.3984 0.4027 0.4362 0.3969 0.3531 0.3388
Nuclear Periphery 0.0197 0.0012 0.0025 0.0156 0.0407 0.0041 0.023 0.0025 0.0015 0.0026 0.0262 0.0016 0.0054 0.0002 0.0015 0.0003 0.0006 0.0033
Nucleolus 0.0023 0.0004 0.0004 0.0025 0.0018 0.0003 0.0015 0.0003 0.0027 0.0006 0.0003 0.0005 0.0012 0.0003 0.0006 0.0014 0.0004 0.0007
Nucleus 0.0174 0.0011 0.001 0.0183 0.0096 0.0007 0.0071 0.0014 0.0058 0.0015 0.0034 0.0052 0.0084 0.0014 0.0039 0.0021 0.0014 0.0035
Peroxisomes 0.012 0.0002 0.0094 0.0045 0.013 0.0021 0.0079 0.0001 0.0035 0.004 0.0032 0.0018 0.0226 0.0014 0.0069 0.0013 0.0049 0.0067
Punctate Nuclear 0.0193 0.0012 0.0012 0.0318 0.0176 0.0051 0.0048 0.0005 0.0015 0.0011 0.0011 0.002 0.0025 0.0008 0.001 0.0007 0.0002 0.0056
Vacuole 0.0048 0.0016 0.0022 0.0049 0.003 0.0019 0.0095 0.0026 0.0122 0.0078 0.004 0.0027 0.005 0.0014 0.0026 0.0053 0.0106 0.0012
Vacuole Periphery 0.0019 0.0001 0.0006 0.0011 0.0016 0.0004 0.0024 0.0006 0.0018 0.0019 0.0037 0.0003 0.0012 0.0001 0.0005 0.0005 0.0029 0.0006

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 7.721 11.7127 9.9329 14.4619 11.2241 14.0305 9.8337 16.557 16.6892 13.3595
Translational Efficiency 0.9563 0.6918 1.1589 0.9896 0.8788 0.9589 1.2079 0.8586 1.0759 1.0506

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1365 1151 171 815 1466 1549 123 2421 2831 2700 294 3236

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 649.79 716.93 913.84 827.29 617.78 682.34 884.74 802.54 633.21 697.09 901.67 808.77
Standard Deviation 68.42 95.66 97.98 118.70 69.60 80.42 103.45 107.90 70.87 88.90 101.33 111.24
Intensity Change Log 2 — 0.141859 0.491968 0.348420 — 0.143398 0.518160 0.377480 — 0.142609 0.504793 0.362656

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000170 0.000740 0.001210 0.006284 0.000337 0.000734 0.000698 0.000230 0.000256 0.000737 0.000996 0.001755
Bud Neck 0.021223 0.038170 0.011343 0.014815 0.031961 0.054859 0.003093 0.008019 0.026784 0.047745 0.007891 0.009731
Bud Site 0.005964 0.025279 0.007301 0.079792 0.008751 0.026192 0.009481 0.034432 0.007407 0.025803 0.008213 0.045856
Cell Periphery 0.000288 0.000195 0.000523 0.000493 0.000284 0.000224 0.000373 0.000225 0.000286 0.000212 0.000460 0.000292
Cytoplasm 0.572725* 0.453278* 0.140981* 0.392851* 0.487476* 0.436568* 0.339067* 0.647521* 0.528580* 0.443692* 0.223854* 0.583381*
Cytoplasmic Foci 0.161108 0.233369* 0.011615 0.035197 0.256757* 0.240682* 0.026178 0.021790 0.210639* 0.237565* 0.017707 0.025167
Eisosomes 0.000420 0.000215 0.000169 0.000135 0.000590 0.000353 0.000158 0.000052 0.000508 0.000294 0.000164 0.000073
Endoplasmic Reticulum 0.000471 0.000790 0.020046 0.003891 0.000632 0.000474 0.005446 0.000541 0.000554 0.000609 0.013938 0.001385
Endosome 0.007406 0.023994 0.005427 0.022657 0.013412 0.025610 0.006042 0.001281 0.010516 0.024922 0.005684 0.006665
Golgi 0.002309 0.005283 0.000151 0.016502 0.004079 0.006390 0.000110 0.000339 0.003225 0.005918 0.000134 0.004410
Lipid Particles 0.005875 0.005511 0.004563 0.001985 0.008983 0.006105 0.004002 0.000522 0.007484 0.005852 0.004328 0.000890
Mitochondria 0.003271 0.009026 0.000927 0.007702 0.004932 0.008964 0.000476 0.000589 0.004132 0.008990 0.000739 0.002380
Mitotic Spindle 0.000600 0.005264 0.007093 0.056486 0.000134 0.004405 0.001158 0.011357 0.000359 0.004771 0.004610 0.022723
None 0.003045 0.002412 0.002164 0.002930 0.003379 0.001318 0.005256 0.004168 0.003218 0.001784 0.003458 0.003857
Nuclear Periphery 0.000188 0.000268 0.002662 0.001205 0.000227 0.000231 0.001726 0.000536 0.000208 0.000247 0.002271 0.000704
Nuclear Periphery Foci 0.000212 0.000540 0.003480 0.002929 0.000213 0.000378 0.002502 0.000336 0.000212 0.000447 0.003071 0.000989
Nucleolus 0.000427 0.001315 0.000940 0.000544 0.000561 0.000958 0.000431 0.000250 0.000497 0.001110 0.000727 0.000324
Nucleus 0.161858* 0.088815 0.640840* 0.111958 0.109881 0.076254 0.331635* 0.172169* 0.134942* 0.081609 0.511479* 0.157005*
Peroxisomes 0.002721 0.015139 0.000544 0.001549 0.009119 0.015964 0.000373 0.000715 0.006034 0.015612 0.000472 0.000925
Vacuole 0.049335 0.088107 0.137764 0.228183* 0.057508 0.091277 0.261620* 0.094428 0.053567 0.089926 0.189581 0.128114
Vacuole Periphery 0.000383 0.002289 0.000257 0.011913 0.000782 0.002058 0.000175 0.000500 0.000589 0.002157 0.000223 0.003375

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -3.94 -8.51 -3.38 -2.61 -1.60 -2.84 -2.00 3.28 3.72 2.34 -4.73 -6.16 -3.09 -0.57 1.74
Bud Neck -5.18 1.88 6.23 9.61 2.64 -6.65 11.65 12.25 16.49 -1.49 -8.71 7.90 14.08 19.26 2.46
Bud Site -7.76 -2.50 -8.33 -3.88 -7.39 -7.61 -0.44 -6.01 1.73 -1.94 -10.86 -1.28 -10.13 -0.59 -5.86
Cell Periphery 3.42 -5.36 -1.78 -6.14 4.30 3.10 -1.16 5.58 2.61 4.67 4.49 -5.37 3.64 -1.02 7.85
Cytoplasm 11.82 32.34 21.71 10.04 -12.71 5.18 12.86 -5.69 -11.05 -15.34 12.08 29.76 6.28 -5.46 -25.67
Cytoplasmic Foci -9.49 33.19 30.57 33.16 -5.96 2.10 39.28 43.12 40.61 -0.75 -4.82 50.68 51.87 52.77 -4.14
Eisosomes 7.55 8.15 11.15 4.28 3.43 5.26 9.87 13.30 13.32 8.61 7.90 12.53 18.00 14.84 10.46
Endoplasmic Reticulum -2.67 -12.00 -7.30 -6.56 9.87 1.84 -8.40 -4.87 -7.98 7.99 -0.89 -13.47 -8.88 -8.60 12.42
Endosome -8.64 1.26 -0.37 6.72 -1.10 -7.48 7.55 14.65 17.52 7.27 -11.63 6.45 11.37 18.89 5.26
Golgi -3.13 3.82 -1.55 0.09 -2.98 -3.75 13.13 12.55 11.51 -1.11 -4.93 9.84 3.57 7.53 -3.05
Lipid Particles 0.47 1.14 4.97 6.32 5.51 3.68 6.51 14.87 9.82 7.28 2.76 5.03 13.85 12.35 10.12
Mitochondria -3.04 2.06 0.43 3.79 -2.38 -2.95 4.40 4.58 8.86 0.64 -4.31 4.48 4.09 9.36 -1.05
Mitotic Spindle -2.77 -1.56 -6.41 -4.96 -3.59 -3.77 -1.81 -7.80 -3.46 -4.40 -4.57 -1.95 -9.88 -5.76 -3.60
None 0.76 1.72 1.83 0.63 0.21 2.25 -0.30 -0.31 -6.01 0.10 2.37 0.68 0.00 -3.60 -0.93
Nuclear Periphery -3.68 -14.77 -11.45 -10.46 7.06 -0.15 -9.71 -18.85 -17.05 6.46 -1.85 -17.40 -22.75 -20.30 11.11
Nuclear Periphery Foci -2.30 -7.10 -6.03 -4.53 2.85 -0.99 -6.90 -7.44 -0.38 6.32 -2.09 -9.57 -7.70 -2.94 7.45
Nucleolus -4.01 -3.55 1.25 4.24 3.77 -2.44 -0.57 3.62 3.87 1.18 -4.61 -1.89 3.67 5.78 2.82
Nucleus 11.84 -20.58 2.23 -7.52 20.98 7.33 -9.78 -19.55 -26.68 5.66 14.03 -20.64 -14.40 -26.73 16.81
Peroxisomes -8.96 5.74 5.30 10.88 -1.10 -4.60 10.25 10.03 12.66 -0.26 -9.33 11.54 11.20 16.68 -0.01
Vacuole -10.03 -7.91 -30.72 -25.71 -10.85 -8.13 -10.75 -22.29 -13.99 6.57 -12.83 -12.61 -35.05 -25.19 1.54
Vacuole Periphery -4.71 2.08 -3.46 -1.39 -3.60 -4.71 9.45 3.61 6.10 -0.84 -6.76 8.15 -2.08 2.94 -3.53
ORF
Human Ortholog
Description Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm
Localization
Cell Percentages cytoplasm (49%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

YGR237C

YGR237C


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ygr237c-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available