Standard name | |
---|---|
Human Ortholog | |
Description | Peroxin required for peroxisomal matrix protein targeting; acts on proteins containing the PTS2 targeting sequence; interacts with Pex7p; constitutively expressed; partially redundant with Pex18p; required for import of the Gpd1p-Pnc1p heterodimer in which only Gpd1p has a peroxisomal targeting signal; relative distribution to cytoplasmic foci increases upon DNA replication stress |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 37 | 94 | 183 | 80 | 29 | 24 | 9 | – | 4 | 1 | 2 | 0 | 66 | 54 | 16 | 4 | 1 | – | 14 | 82 | 101 | 51 | 67 | 75 |
Bud | 19 | 49 | 45 | 38 | 42 | 38 | 34 | – | 45 | 19 | 27 | 25 | 19 | 26 | 14 | 2 | 2 | – | 0 | 4 | 5 | 5 | 2 | 1 |
Bud Neck | 0 | 1 | 2 | 0 | 3 | 3 | 5 | – | 7 | 5 | 0 | 3 | 0 | 0 | 1 | 0 | 0 | – | 1 | 5 | 4 | 3 | 5 | 9 |
Bud Site | 1 | 12 | 5 | 2 | 3 | 10 | 3 | – | 7 | 6 | 5 | 6 | 3 | 0 | 2 | 2 | 0 | – | – | – | – | – | – | – |
Cell Periphery | 1 | 8 | 9 | 1 | 1 | 1 | 2 | – | 0 | 1 | 1 | 1 | 3 | 4 | 0 | 0 | 0 | – | 0 | 0 | 1 | 0 | 0 | 1 |
Cytoplasm | 11 | 43 | 39 | 35 | 72 | 148 | 204 | – | 235 | 186 | 126 | 138 | 27 | 50 | 100 | 6 | 1 | – | 0 | 5 | 5 | 9 | 7 | 3 |
Endoplasmic Reticulum | 7 | 17 | 5 | 9 | 4 | 8 | 5 | – | 1 | 0 | 1 | 0 | 10 | 18 | 10 | 1 | 0 | – | 0 | 2 | 2 | 2 | 1 | 2 |
Endosome | 19 | 79 | 84 | 54 | 43 | 70 | 80 | – | 33 | 19 | 11 | 5 | 48 | 66 | 83 | 9 | 4 | – | 29 | 138 | 112 | 73 | 57 | 62 |
Golgi | 21 | 27 | 44 | 11 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 18 | 5 | 2 | 1 | 3 | – | 7 | 25 | 12 | 7 | 8 | 5 |
Mitochondria | 57 | 132 | 4 | 27 | 41 | 32 | 28 | – | 182 | 112 | 105 | 104 | 4 | 8 | 7 | 2 | 4 | – | 2 | 29 | 14 | 14 | 21 | 15 |
Nucleus | 1 | 0 | 1 | 0 | 3 | 1 | 2 | – | 6 | 3 | 3 | 4 | 2 | 4 | 2 | 0 | 0 | – | 0 | 0 | 1 | 30 | 29 | 33 |
Nuclear Periphery | 1 | 0 | 1 | 1 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | – | 0 | 1 | 1 | 1 | 1 | 0 |
Nucleolus | 2 | 3 | 1 | 0 | 1 | 1 | 0 | – | 5 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 0 | – | 0 | 0 | 0 | 10 | 15 | 13 |
Peroxisomes | 22 | 101 | 90 | 68 | 15 | 17 | 15 | – | 11 | 11 | 11 | 4 | 58 | 38 | 42 | 14 | 4 | – | 99 | 200 | 307 | 128 | 151 | 125 |
SpindlePole | 10 | 44 | 205 | 67 | 71 | 84 | 92 | – | 65 | 44 | 25 | 42 | 66 | 92 | 59 | 14 | 6 | – | 5 | 38 | 49 | 24 | 20 | 18 |
Vac/Vac Membrane | 4 | 15 | 15 | 3 | 10 | 14 | 18 | – | 15 | 11 | 4 | 5 | 0 | 1 | 5 | 2 | 0 | – | 0 | 6 | 0 | 7 | 9 | 9 |
Unique Cell Count | 130 | 422 | 475 | 260 | 249 | 360 | 402 | 488 | 335 | 260 | 266 | 223 | 261 | 279 | 42 | 16 | 163 | 544 | 629 | 380 | 403 | 388 | ||
Labelled Cell Count | 213 | 625 | 733 | 396 | 338 | 451 | 497 | 616 | 419 | 321 | 337 | 326 | 368 | 344 | 58 | 25 | 163 | 544 | 629 | 380 | 403 | 388 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 7.2 | 6.0 | 7.7 | 7.0 | 5.7 | 5.3 | 5.1 | – | 4.1 | 4.4 | 4.2 | 4.1 | 7.3 | 6.4 | 6.0 | 6.9 | 8.2 | – | 7.7 | 7.8 | 7.8 |
Std Deviation (1e-4) | 1.3 | 1.0 | 1.2 | 1.5 | 1.7 | 1.1 | 1.1 | – | 1.2 | 1.4 | 1.3 | 1.2 | 1.1 | 1.3 | 1.3 | 1.8 | 1.6 | – | 1.7 | 1.6 | 1.6 |
Intensity Change (Log2) | – | – | – | -0.13 | -0.43 | -0.54 | -0.59 | – | -0.9 | -0.79 | -0.87 | -0.89 | -0.07 | -0.26 | -0.35 | -0.15 | 0.1 | – | 0.0 | 0.02 | 0.02 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | -2.1 | -7.5 | -10.6 | -12.9 | – | 0 | 0 | 0 | 0 | -2.3 | -5.0 | -9.9 | -3.7 | -2.6 | – |
Bud | 2.1 | 2.9 | 0.5 | -0.5 | – | 0 | 0 | 0 | 0 | -0.4 | 0.2 | -2.2 | 0 | 0 | – |
Bud Neck | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – |
Bud Site | 0 | 0 | 1.9 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – |
Cell Periphery | 0 | 0 | 0 | -1.9 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – |
Cytoplasm | 2.3 | 7.3 | 11.3 | 14.0 | – | 13.7 | 14.8 | 12.5 | 13.4 | 1.6 | 4.4 | 9.4 | 0 | 0 | – |
Endoplasmic Reticulum | 0 | 0 | 1.4 | 0 | – | 0 | 0 | 0 | 0 | 0 | 4.4 | 2.4 | 0 | 0 | – |
Endosome | 1.0 | -0.1 | 0.6 | 0.8 | – | 0 | 0 | 0 | 0 | 1.2 | 2.4 | 3.9 | 0.6 | 0 | – |
Golgi | -2.5 | -5.0 | -5.9 | -6.3 | – | 0 | 0 | 0 | 0 | -0.5 | -3.8 | -4.7 | 0 | 0 | – |
Mitochondria | 6.2 | 8.3 | 5.7 | 4.8 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – |
Nucleus | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – |
Nuclear Periphery | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – |
Nucleolus | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – |
Peroxisomes | 2.3 | -4.7 | -6.1 | -6.9 | – | 0 | 0 | 0 | 0 | 2.1 | -1.5 | -1.4 | 2.2 | 0 | – |
SpindlePole | -4.7 | -3.9 | -6.0 | -6.3 | – | 0 | 0 | 0 | 0 | -3.4 | -2.1 | -6.1 | -1.2 | -0.4 | – |
Vacuole | -1.7 | 0.6 | 0.6 | 1.0 | – | -0.1 | 0.1 | -1.3 | -1.0 | 0 | -2.5 | -1.1 | 0 | 0 | – |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 8.6645 | 9.2587 | 9.2487 | 9.3353 | 8.5334 | 9.4116 | 9.5454 | 12.0131 | 10.4724 | 10.0611 | 10.4228 | 10.511 | 5.7275 | 6.8314 | 6.1903 | 6.3102 | 6.048 | 6.4235 |
Actin | 0.153 | 0.1555 | 0.1635 | 0.0701 | 0.0729 | 0.1183 | 0.2071 | 0.1539 | 0.2315 | 0.1578 | 0.0624 | 0.209 | 0.1809 | 0.1732 | 0.1811 | 0.1587 | 0.0432 | 0.1011 |
Bud | 0.0004 | 0.0027 | 0.0009 | 0.0008 | 0.0006 | 0.0003 | 0.0004 | 0.0013 | 0.0006 | 0.0004 | 0.0051 | 0.0006 | 0.0003 | 0.0006 | 0.0007 | 0.0003 | 0.0002 | 0.002 |
Bud Neck | 0.0011 | 0.0008 | 0.0027 | 0.0018 | 0.0009 | 0.0023 | 0.0008 | 0.0027 | 0.0023 | 0.001 | 0.0038 | 0.0034 | 0.0011 | 0.0018 | 0.0084 | 0.001 | 0.0222 | 0.0048 |
Bud Periphery | 0.0004 | 0.0017 | 0.001 | 0.0006 | 0.0007 | 0.0002 | 0.0004 | 0.001 | 0.0004 | 0.0001 | 0.0063 | 0.0003 | 0.0002 | 0.0003 | 0.0004 | 0.0001 | 0.0001 | 0.0013 |
Bud Site | 0.0024 | 0.0205 | 0.0033 | 0.0013 | 0.001 | 0.0007 | 0.0012 | 0.0235 | 0.0015 | 0.0011 | 0.0213 | 0.0008 | 0.002 | 0.0088 | 0.0033 | 0.0006 | 0.0006 | 0.0006 |
Cell Periphery | 0.0001 | 0 | 0.0001 | 0.0001 | 0 | 0 | 0.0001 | 0.0003 | 0 | 0 | 0.0005 | 0.0001 | 0 | 0.0001 | 0.0001 | 0 | 0 | 0 |
Cytoplasm | 0.0045 | 0.0366 | 0.0185 | 0.0166 | 0.0021 | 0.0202 | 0.0293 | 0.1208 | 0.049 | 0.056 | 0.0125 | 0.0577 | 0.0238 | 0.0865 | 0.0463 | 0.0515 | 0.043 | 0.0233 |
Cytoplasmic Foci | 0.1732 | 0.2225 | 0.1647 | 0.2502 | 0.2205 | 0.2184 | 0.1509 | 0.2366 | 0.1805 | 0.2422 | 0.3493 | 0.2185 | 0.1963 | 0.2931 | 0.1795 | 0.2301 | 0.2484 | 0.2698 |
Eisosomes | 0.0004 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0002 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0 | 0 | 0.0001 |
Endoplasmic Reticulum | 0.002 | 0.0003 | 0.0025 | 0.0005 | 0.0005 | 0.0009 | 0.0037 | 0.0017 | 0.0003 | 0.0028 | 0.0015 | 0.001 | 0.0004 | 0.0007 | 0.0007 | 0.0013 | 0.0001 | 0.0005 |
Endosome | 0.0577 | 0.0375 | 0.0238 | 0.0683 | 0.0233 | 0.0349 | 0.052 | 0.0242 | 0.0137 | 0.0809 | 0.0613 | 0.0236 | 0.0331 | 0.0335 | 0.0187 | 0.0285 | 0.0538 | 0.0419 |
Golgi | 0.0625 | 0.0455 | 0.0488 | 0.0695 | 0.0516 | 0.0542 | 0.0264 | 0.0221 | 0.0223 | 0.0437 | 0.0382 | 0.0294 | 0.0325 | 0.0513 | 0.0418 | 0.0429 | 0.0374 | 0.0444 |
Lipid Particles | 0.0192 | 0.0054 | 0.0056 | 0.0048 | 0.014 | 0.0114 | 0.0097 | 0.0029 | 0.0083 | 0.0146 | 0.0052 | 0.0044 | 0.0104 | 0.0092 | 0.0041 | 0.0028 | 0.0085 | 0.0115 |
Mitochondria | 0.0103 | 0.0018 | 0.0028 | 0.0047 | 0.0065 | 0.0041 | 0.0034 | 0.0013 | 0.002 | 0.002 | 0.0033 | 0.0024 | 0.0017 | 0.0017 | 0.002 | 0.0016 | 0.0016 | 0.002 |
None | 0.0013 | 0.0013 | 0.0011 | 0.0005 | 0.0006 | 0.0006 | 0.0206 | 0.0026 | 0.0009 | 0.0062 | 0.0004 | 0.0018 | 0.0009 | 0.0029 | 0.0009 | 0.0003 | 0.0003 | 0.0032 |
Nuclear Periphery | 0.0096 | 0.0003 | 0.0003 | 0.0001 | 0.0005 | 0.0008 | 0.0109 | 0.0003 | 0.0003 | 0.0016 | 0.0008 | 0.0004 | 0.0001 | 0.0002 | 0.0004 | 0.0001 | 0 | 0.0002 |
Nucleolus | 0.0002 | 0.0001 | 0 | 0 | 0.0001 | 0 | 0.0005 | 0.0001 | 0 | 0.0002 | 0.0003 | 0 | 0 | 0.0001 | 0.0001 | 0 | 0 | 0.0001 |
Nucleus | 0.0005 | 0.0008 | 0.0002 | 0.0002 | 0.0001 | 0.0004 | 0.0054 | 0.0004 | 0.0001 | 0.0009 | 0.0008 | 0.0004 | 0.0001 | 0.0002 | 0.0026 | 0.0001 | 0.0001 | 0.0004 |
Peroxisomes | 0.4959 | 0.4584 | 0.5573 | 0.5064 | 0.6003 | 0.5293 | 0.4651 | 0.3991 | 0.4759 | 0.3777 | 0.4213 | 0.4382 | 0.5146 | 0.3311 | 0.5032 | 0.4793 | 0.5392 | 0.4891 |
Punctate Nuclear | 0.0038 | 0.0078 | 0.0025 | 0.0022 | 0.0033 | 0.0025 | 0.0095 | 0.005 | 0.0102 | 0.0093 | 0.0028 | 0.0073 | 0.0011 | 0.0039 | 0.0052 | 0.0007 | 0.0008 | 0.0032 |
Vacuole | 0.0005 | 0.0002 | 0.0002 | 0.0008 | 0.0002 | 0.0002 | 0.0013 | 0.0002 | 0.0001 | 0.0008 | 0.0023 | 0.0003 | 0.0002 | 0.0003 | 0.0001 | 0.0001 | 0.0004 | 0.0003 |
Vacuole Periphery | 0.0009 | 0.0001 | 0.0001 | 0.0005 | 0.0002 | 0.0001 | 0.001 | 0.0001 | 0.0001 | 0.0004 | 0.0006 | 0.0002 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0002 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 29.9077 | 17.0653 | 11.819 | 15.4356 | 12.3755 | 29.2552 | 33.3592 | 30.279 | 21.0739 | 23.3432 |
Translational Efficiency | 0.8392 | 1.3448 | 1.3801 | 0.7204 | 1.4128 | 1.6171 | 1.0764 | 0.8654 | 1.2318 | 1.2661 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✔ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
2475 | 1726 | 2893 | 104 | 1740 | 2371 | 1103 | 214 | 4215 | 4097 | 3996 | 318 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 809.53 | 885.55 | 911.47 | 944.08 | 800.25 | 937.27 | 859.96 | 871.59 | 805.70 | 915.48 | 897.25 | 895.30 |
Standard Deviation | 97.64 | 124.97 | 130.06 | 152.71 | 91.26 | 120.51 | 124.76 | 118.23 | 95.17 | 125.05 | 130.66 | 134.87 |
Intensity Change Log 2 | — | 0.129489 | 0.171111 | 0.221825 | — | 0.228014 | 0.103819 | 0.123199 | — | 0.179309 | 0.138051 | 0.173638 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.033071 | 0.060674 | 0.148898* | 0.116981* | 0.034695 | 0.030353 | 0.040751 | 0.021031 | 0.033742 | 0.043127 | 0.119046* | 0.052411 |
Bud Neck | 0.003963 | 0.001901 | 0.002637 | 0.003412 | 0.001880 | 0.001403 | 0.004381 | 0.010389 | 0.003103 | 0.001613 | 0.003119 | 0.008107 |
Bud Site | 0.007047 | 0.009527 | 0.023922 | 0.040006 | 0.002552 | 0.003157 | 0.022689 | 0.028400 | 0.005191 | 0.005841 | 0.023582 | 0.032196 |
Cell Periphery | 0.000083 | 0.000054 | 0.000068 | 0.000187 | 0.000076 | 0.000051 | 0.000155 | 0.000230 | 0.000080 | 0.000052 | 0.000092 | 0.000216 |
Cytoplasm | 0.006075 | 0.005689 | 0.014948 | 0.019150 | 0.007322 | 0.003021 | 0.021757 | 0.018437 | 0.006590 | 0.004145 | 0.016827 | 0.018670 |
Cytoplasmic Foci | 0.136797 | 0.149135 | 0.091389 | 0.174684 | 0.157164 | 0.133263 | 0.207265* | 0.260272* | 0.145205 | 0.139949 | 0.123374 | 0.232281* |
Eisosomes | 0.000167 | 0.000085 | 0.000096 | 0.000212 | 0.000228 | 0.000119 | 0.000156 | 0.000214 | 0.000192 | 0.000104 | 0.000113 | 0.000213 |
Endoplasmic Reticulum | 0.000040 | 0.000092 | 0.000204 | 0.001220 | 0.000053 | 0.000028 | 0.000629 | 0.000271 | 0.000045 | 0.000055 | 0.000322 | 0.000581 |
Endosome | 0.020674 | 0.040559 | 0.016350 | 0.045657 | 0.040398 | 0.036794 | 0.045299 | 0.077008 | 0.028816 | 0.038380 | 0.024341 | 0.066755 |
Golgi | 0.041610 | 0.043144 | 0.012148 | 0.021721 | 0.043358 | 0.041826 | 0.017559 | 0.018085 | 0.042332 | 0.042381 | 0.013642 | 0.019274 |
Lipid Particles | 0.008439 | 0.010988 | 0.005606 | 0.011217 | 0.025360 | 0.022547 | 0.029969 | 0.048873 | 0.015424 | 0.017678 | 0.012331 | 0.036558 |
Mitochondria | 0.004997 | 0.002380 | 0.001995 | 0.002713 | 0.004139 | 0.003253 | 0.001511 | 0.001117 | 0.004643 | 0.002886 | 0.001861 | 0.001639 |
Mitotic Spindle | 0.002632 | 0.011198 | 0.021360 | 0.009333 | 0.003341 | 0.002311 | 0.034473 | 0.007387 | 0.002925 | 0.006055 | 0.024979 | 0.008023 |
None | 0.000120 | 0.000041 | 0.000031 | 0.000061 | 0.000061 | 0.000050 | 0.000524 | 0.000224 | 0.000096 | 0.000046 | 0.000167 | 0.000170 |
Nuclear Periphery | 0.000003 | 0.000007 | 0.000016 | 0.000068 | 0.000009 | 0.000004 | 0.000053 | 0.000055 | 0.000006 | 0.000005 | 0.000026 | 0.000060 |
Nuclear Periphery Foci | 0.000051 | 0.000153 | 0.000242 | 0.003052 | 0.000107 | 0.000106 | 0.000906 | 0.000792 | 0.000074 | 0.000126 | 0.000426 | 0.001531 |
Nucleolus | 0.000036 | 0.000030 | 0.000023 | 0.000153 | 0.000239 | 0.000012 | 0.000394 | 0.000751 | 0.000120 | 0.000020 | 0.000126 | 0.000555 |
Nucleus | 0.000343 | 0.000076 | 0.000707 | 0.001835 | 0.000603 | 0.000054 | 0.006322 | 0.011088 | 0.000450 | 0.000063 | 0.002257 | 0.008062 |
Peroxisomes | 0.733168* | 0.663383* | 0.656687* | 0.518908* | 0.677262* | 0.721234* | 0.555681* | 0.482867* | 0.710089* | 0.696862* | 0.628807* | 0.494654* |
Vacuole | 0.000629 | 0.000804 | 0.002609 | 0.028670 | 0.001059 | 0.000341 | 0.009217 | 0.012326 | 0.000807 | 0.000536 | 0.004433 | 0.017671 |
Vacuole Periphery | 0.000054 | 0.000081 | 0.000063 | 0.000758 | 0.000094 | 0.000073 | 0.000308 | 0.000185 | 0.000070 | 0.000076 | 0.000131 | 0.000372 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -6.95 | -23.63 | -3.81 | -2.49 | 1.58 | 1.34 | -1.14 | 3.00 | 2.22 | 3.64 | -3.89 | -22.39 | -2.19 | -0.99 | 7.88 |
Bud Neck | 2.46 | 1.45 | 0.36 | -1.73 | -0.82 | 1.01 | -3.07 | -3.23 | -3.43 | -2.19 | 2.89 | -0.28 | -2.74 | -3.63 | -2.70 |
Bud Site | -1.70 | -10.52 | -3.27 | -3.03 | -1.64 | -0.92 | -8.22 | -3.26 | -3.18 | -0.52 | -0.84 | -14.07 | -4.35 | -4.24 | -1.30 |
Cell Periphery | 2.01 | 0.83 | -3.29 | -4.44 | -3.92 | 1.74 | -4.43 | -3.13 | -3.70 | -1.37 | 2.64 | -1.51 | -3.96 | -4.89 | -3.59 |
Cytoplasm | 0.38 | -8.19 | -2.34 | -2.39 | -0.69 | 3.19 | -6.42 | -2.74 | -3.80 | 1.20 | 3.05 | -10.02 | -3.72 | -4.42 | -0.30 |
Cytoplasmic Foci | -2.22 | 10.51 | -2.17 | -1.49 | -4.70 | 4.54 | -7.11 | -6.91 | -8.61 | -3.44 | 1.54 | 5.52 | -7.47 | -7.96 | -9.18 |
Eisosomes | 3.67 | 3.09 | -1.52 | -5.11 | -4.62 | 3.35 | 2.06 | 0.25 | -2.45 | -1.39 | 4.55 | 4.14 | -0.76 | -4.04 | -3.71 |
Endoplasmic Reticulum | -2.38 | -3.55 | -2.36 | -2.26 | -1.99 | 1.86 | -3.76 | -3.35 | -3.73 | 2.31 | -0.88 | -5.13 | -3.12 | -3.06 | -1.28 |
Endosome | -7.95 | 2.55 | -2.42 | -0.43 | -2.85 | 1.16 | -1.15 | -3.67 | -4.11 | -3.17 | -4.94 | 2.60 | -5.10 | -3.79 | -5.73 |
Golgi | -0.57 | 14.35 | 2.89 | 3.11 | -1.24 | 0.52 | 10.84 | 8.92 | 8.89 | -0.38 | -0.03 | 18.57 | 7.90 | 8.04 | -1.98 |
Lipid Particles | -1.73 | 2.57 | -0.98 | -0.08 | -1.95 | 1.06 | -1.65 | -2.66 | -2.99 | -1.97 | -1.39 | 1.94 | -3.48 | -3.11 | -3.90 |
Mitochondria | 2.86 | 3.44 | 1.14 | -0.12 | -0.29 | 0.96 | 3.29 | 3.56 | 3.03 | 0.87 | 2.65 | 4.41 | 3.46 | 1.60 | 0.39 |
Mitotic Spindle | -5.98 | -12.75 | -2.50 | 0.54 | 3.92 | 1.48 | -9.52 | -1.77 | -2.25 | 7.21 | -4.65 | -15.68 | -2.94 | -1.06 | 8.00 |
None | 1.26 | 1.36 | 0.89 | -1.17 | -1.57 | 0.44 | -2.03 | -1.77 | -1.85 | 1.26 | 1.26 | -1.08 | -1.06 | -1.98 | 0.06 |
Nuclear Periphery | -2.24 | -4.13 | -2.63 | -2.52 | -1.92 | 3.65 | -6.84 | -3.35 | -3.70 | 1.08 | 0.86 | -7.35 | -4.36 | -4.41 | -1.88 |
Nuclear Periphery Foci | -1.25 | -2.10 | -1.44 | -1.41 | -1.26 | 0.47 | -4.09 | -2.48 | -2.59 | 1.54 | -0.38 | -4.06 | -2.07 | -2.05 | -1.14 |
Nucleolus | 1.18 | 0.94 | -1.50 | -2.30 | -2.17 | 1.30 | -0.32 | -0.87 | -3.16 | -0.67 | 1.46 | 0.24 | -1.70 | -3.36 | -2.24 |
Nucleus | 2.07 | -1.76 | -2.53 | -2.98 | -1.46 | 2.48 | -4.35 | -2.59 | -2.73 | -0.75 | 3.27 | -4.55 | -2.81 | -2.95 | -1.90 |
Peroxisomes | 7.56 | 10.24 | 7.37 | 5.06 | 4.73 | -4.87 | 11.37 | 8.60 | 10.68 | 2.60 | 1.96 | 13.40 | 12.12 | 11.42 | 7.25 |
Vacuole | -0.71 | -6.46 | -3.18 | -3.17 | -2.95 | 3.22 | -6.87 | -2.97 | -3.15 | -0.16 | 1.74 | -9.16 | -4.30 | -4.36 | -3.22 |
Vacuole Periphery | -1.07 | -0.75 | -1.29 | -1.23 | -1.26 | 0.69 | -1.29 | -2.01 | -2.24 | 0.70 | -0.26 | -1.31 | -1.68 | -1.64 | -1.30 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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