Standard name
Human Ortholog
Description Peroxin required for peroxisomal matrix protein targeting; acts on proteins containing the PTS2 targeting sequence; interacts with Pex7p; constitutively expressed; partially redundant with Pex18p; required for import of the Gpd1p-Pnc1p heterodimer in which only Gpd1p has a peroxisomal targeting signal; relative distribution to cytoplasmic foci increases upon DNA replication stress
DOWNLOAD

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.28 0.22 0.39 0.31 0.12 0.07 0 – 0 0 0 0 0.3 0.21 0.06 0.1 0.06 – 0.09 0.15 0.16 0.14 0.17 0.19
Bud 0.15 0.12 0.09 0.15 0.17 0.11 0.08 – 0.09 0.06 0.1 0.09 0.09 0.1 0.05 0.05 0.12 – 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0.05 0 – – – – – – –
Cell Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
Cytoplasm 0.08 0.1 0.08 0.13 0.29 0.41 0.51 – 0.48 0.56 0.48 0.52 0.12 0.19 0.36 0.14 0.06 – 0 0 0 0 0 0
Endoplasmic Reticulum 0.05 0 0 0 0 0 0 – 0 0 0 0 0 0.07 0 0 0 – 0 0 0 0 0 0
Endosome 0.15 0.19 0.18 0.21 0.17 0.19 0.2 – 0.07 0.06 0 0 0.22 0.25 0.3 0.21 0.25 – 0.18 0.26 0.18 0.19 0.14 0.16
Golgi 0.16 0.06 0.09 0 0 0 0 – 0 0 0 0 0.08 0 0 0 0.19 – 0.05 0.05 0 0 0 0
Mitochondria 0.44 0.31 0 0.1 0.16 0.09 0.07 – 0.37 0.33 0.4 0.39 0 0 0 0.05 0.25 – 0 0.05 0 0 0.05 0
Nucleus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 – 0 0 0 0.08 0.07 0.09
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
Peroxisomes 0.17 0.24 0.19 0.26 0.06 0.05 0 – 0 0 0 0 0.26 0.15 0.15 0.33 0.25 – 0.61 0.37 0.49 0.34 0.38 0.32
SpindlePole 0.08 0.1 0.43 0.26 0.29 0.23 0.23 – 0.13 0.13 0.1 0.16 0.3 0.35 0.21 0.33 0.38 – 0 0.07 0.08 0.06 0.05 0.05
Vac/Vac Membrane 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0.05 0 – 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 37 94 183 80 29 24 9 – 4 1 2 0 66 54 16 4 1 – 14 82 101 51 67 75
Bud 19 49 45 38 42 38 34 – 45 19 27 25 19 26 14 2 2 – 0 4 5 5 2 1
Bud Neck 0 1 2 0 3 3 5 – 7 5 0 3 0 0 1 0 0 – 1 5 4 3 5 9
Bud Site 1 12 5 2 3 10 3 – 7 6 5 6 3 0 2 2 0 – – – – – – –
Cell Periphery 1 8 9 1 1 1 2 – 0 1 1 1 3 4 0 0 0 – 0 0 1 0 0 1
Cytoplasm 11 43 39 35 72 148 204 – 235 186 126 138 27 50 100 6 1 – 0 5 5 9 7 3
Endoplasmic Reticulum 7 17 5 9 4 8 5 – 1 0 1 0 10 18 10 1 0 – 0 2 2 2 1 2
Endosome 19 79 84 54 43 70 80 – 33 19 11 5 48 66 83 9 4 – 29 138 112 73 57 62
Golgi 21 27 44 11 0 0 0 – 0 0 0 0 18 5 2 1 3 – 7 25 12 7 8 5
Mitochondria 57 132 4 27 41 32 28 – 182 112 105 104 4 8 7 2 4 – 2 29 14 14 21 15
Nucleus 1 0 1 0 3 1 2 – 6 3 3 4 2 4 2 0 0 – 0 0 1 30 29 33
Nuclear Periphery 1 0 1 1 0 0 0 – 0 0 0 0 1 1 0 0 0 – 0 1 1 1 1 0
Nucleolus 2 3 1 0 1 1 0 – 5 1 0 0 1 1 1 1 0 – 0 0 0 10 15 13
Peroxisomes 22 101 90 68 15 17 15 – 11 11 11 4 58 38 42 14 4 – 99 200 307 128 151 125
SpindlePole 10 44 205 67 71 84 92 – 65 44 25 42 66 92 59 14 6 – 5 38 49 24 20 18
Vac/Vac Membrane 4 15 15 3 10 14 18 – 15 11 4 5 0 1 5 2 0 – 0 6 0 7 9 9
Unique Cell Count 130 422 475 260 249 360 402 488 335 260 266 223 261 279 42 16 163 544 629 380 403 388
Labelled Cell Count 213 625 733 396 338 451 497 616 419 321 337 326 368 344 58 25 163 544 629 380 403 388


Peroxisomes

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 7.2 6.0 7.7 7.0 5.7 5.3 5.1 – 4.1 4.4 4.2 4.1 7.3 6.4 6.0 6.9 8.2 – 7.7 7.8 7.8
Std Deviation (1e-4) 1.3 1.0 1.2 1.5 1.7 1.1 1.1 – 1.2 1.4 1.3 1.2 1.1 1.3 1.3 1.8 1.6 – 1.7 1.6 1.6
Intensity Change (Log2) – – – -0.13 -0.43 -0.54 -0.59 – -0.9 -0.79 -0.87 -0.89 -0.07 -0.26 -0.35 -0.15 0.1 – 0.0 0.02 0.02


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches -2.1 -7.5 -10.6 -12.9 – 0 0 0 0 -2.3 -5.0 -9.9 -3.7 -2.6 –
Bud 2.1 2.9 0.5 -0.5 – 0 0 0 0 -0.4 0.2 -2.2 0 0 –
Bud Neck 0 0 0 0 – 0 0 0 0 0 0 0 0 0 –
Bud Site 0 0 1.9 0 – 0 0 0 0 0 0 0 0 0 –
Cell Periphery 0 0 0 -1.9 – 0 0 0 0 0 0 0 0 0 –
Cytoplasm 2.3 7.3 11.3 14.0 – 13.7 14.8 12.5 13.4 1.6 4.4 9.4 0 0 –
Endoplasmic Reticulum 0 0 1.4 0 – 0 0 0 0 0 4.4 2.4 0 0 –
Endosome 1.0 -0.1 0.6 0.8 – 0 0 0 0 1.2 2.4 3.9 0.6 0 –
Golgi -2.5 -5.0 -5.9 -6.3 – 0 0 0 0 -0.5 -3.8 -4.7 0 0 –
Mitochondria 6.2 8.3 5.7 4.8 – 0 0 0 0 0 0 0 0 0 –
Nucleus 0 0 0 0 – 0 0 0 0 0 0 0 0 0 –
Nuclear Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0 0 –
Nucleolus 0 0 0 0 – 0 0 0 0 0 0 0 0 0 –
Peroxisomes 2.3 -4.7 -6.1 -6.9 – 0 0 0 0 2.1 -1.5 -1.4 2.2 0 –
SpindlePole -4.7 -3.9 -6.0 -6.3 – 0 0 0 0 -3.4 -2.1 -6.1 -1.2 -0.4 –
Vacuole -1.7 0.6 0.6 1.0 – -0.1 0.1 -1.3 -1.0 0 -2.5 -1.1 0 0 –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 8.6645 9.2587 9.2487 9.3353 8.5334 9.4116 9.5454 12.0131 10.4724 10.0611 10.4228 10.511 5.7275 6.8314 6.1903 6.3102 6.048 6.4235
Actin 0.153 0.1555 0.1635 0.0701 0.0729 0.1183 0.2071 0.1539 0.2315 0.1578 0.0624 0.209 0.1809 0.1732 0.1811 0.1587 0.0432 0.1011
Bud 0.0004 0.0027 0.0009 0.0008 0.0006 0.0003 0.0004 0.0013 0.0006 0.0004 0.0051 0.0006 0.0003 0.0006 0.0007 0.0003 0.0002 0.002
Bud Neck 0.0011 0.0008 0.0027 0.0018 0.0009 0.0023 0.0008 0.0027 0.0023 0.001 0.0038 0.0034 0.0011 0.0018 0.0084 0.001 0.0222 0.0048
Bud Periphery 0.0004 0.0017 0.001 0.0006 0.0007 0.0002 0.0004 0.001 0.0004 0.0001 0.0063 0.0003 0.0002 0.0003 0.0004 0.0001 0.0001 0.0013
Bud Site 0.0024 0.0205 0.0033 0.0013 0.001 0.0007 0.0012 0.0235 0.0015 0.0011 0.0213 0.0008 0.002 0.0088 0.0033 0.0006 0.0006 0.0006
Cell Periphery 0.0001 0 0.0001 0.0001 0 0 0.0001 0.0003 0 0 0.0005 0.0001 0 0.0001 0.0001 0 0 0
Cytoplasm 0.0045 0.0366 0.0185 0.0166 0.0021 0.0202 0.0293 0.1208 0.049 0.056 0.0125 0.0577 0.0238 0.0865 0.0463 0.0515 0.043 0.0233
Cytoplasmic Foci 0.1732 0.2225 0.1647 0.2502 0.2205 0.2184 0.1509 0.2366 0.1805 0.2422 0.3493 0.2185 0.1963 0.2931 0.1795 0.2301 0.2484 0.2698
Eisosomes 0.0004 0.0001 0.0001 0.0001 0.0001 0.0001 0.0002 0.0001 0.0001 0.0001 0.0001 0.0001 0.0001 0.0001 0.0001 0 0 0.0001
Endoplasmic Reticulum 0.002 0.0003 0.0025 0.0005 0.0005 0.0009 0.0037 0.0017 0.0003 0.0028 0.0015 0.001 0.0004 0.0007 0.0007 0.0013 0.0001 0.0005
Endosome 0.0577 0.0375 0.0238 0.0683 0.0233 0.0349 0.052 0.0242 0.0137 0.0809 0.0613 0.0236 0.0331 0.0335 0.0187 0.0285 0.0538 0.0419
Golgi 0.0625 0.0455 0.0488 0.0695 0.0516 0.0542 0.0264 0.0221 0.0223 0.0437 0.0382 0.0294 0.0325 0.0513 0.0418 0.0429 0.0374 0.0444
Lipid Particles 0.0192 0.0054 0.0056 0.0048 0.014 0.0114 0.0097 0.0029 0.0083 0.0146 0.0052 0.0044 0.0104 0.0092 0.0041 0.0028 0.0085 0.0115
Mitochondria 0.0103 0.0018 0.0028 0.0047 0.0065 0.0041 0.0034 0.0013 0.002 0.002 0.0033 0.0024 0.0017 0.0017 0.002 0.0016 0.0016 0.002
None 0.0013 0.0013 0.0011 0.0005 0.0006 0.0006 0.0206 0.0026 0.0009 0.0062 0.0004 0.0018 0.0009 0.0029 0.0009 0.0003 0.0003 0.0032
Nuclear Periphery 0.0096 0.0003 0.0003 0.0001 0.0005 0.0008 0.0109 0.0003 0.0003 0.0016 0.0008 0.0004 0.0001 0.0002 0.0004 0.0001 0 0.0002
Nucleolus 0.0002 0.0001 0 0 0.0001 0 0.0005 0.0001 0 0.0002 0.0003 0 0 0.0001 0.0001 0 0 0.0001
Nucleus 0.0005 0.0008 0.0002 0.0002 0.0001 0.0004 0.0054 0.0004 0.0001 0.0009 0.0008 0.0004 0.0001 0.0002 0.0026 0.0001 0.0001 0.0004
Peroxisomes 0.4959 0.4584 0.5573 0.5064 0.6003 0.5293 0.4651 0.3991 0.4759 0.3777 0.4213 0.4382 0.5146 0.3311 0.5032 0.4793 0.5392 0.4891
Punctate Nuclear 0.0038 0.0078 0.0025 0.0022 0.0033 0.0025 0.0095 0.005 0.0102 0.0093 0.0028 0.0073 0.0011 0.0039 0.0052 0.0007 0.0008 0.0032
Vacuole 0.0005 0.0002 0.0002 0.0008 0.0002 0.0002 0.0013 0.0002 0.0001 0.0008 0.0023 0.0003 0.0002 0.0003 0.0001 0.0001 0.0004 0.0003
Vacuole Periphery 0.0009 0.0001 0.0001 0.0005 0.0002 0.0001 0.001 0.0001 0.0001 0.0004 0.0006 0.0002 0.0001 0.0001 0.0001 0.0001 0.0001 0.0002

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 29.9077 17.0653 11.819 15.4356 12.3755 29.2552 33.3592 30.279 21.0739 23.3432
Translational Efficiency 0.8392 1.3448 1.3801 0.7204 1.4128 1.6171 1.0764 0.8654 1.2318 1.2661

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘
Standard name
Human Ortholog
Description Peroxin required for peroxisomal matrix protein targeting; acts on proteins containing the PTS2 targeting sequence; interacts with Pex7p; constitutively expressed; partially redundant with Pex18p; required for import of the Gpd1p-Pnc1p heterodimer in which only Gpd1p has a peroxisomal targeting signal; relative distribution to cytoplasmic foci increases upon DNA replication stress
Localization
Cell Percentages peroxisome (37%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Pex21

Pex21


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Pex21-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available