Standard name
Human Ortholog
Description Highly conserved subunit of the mitochondrial pyruvate carrier (MPC); expressed during growth on nonfermentable carbon sources, and heterodimerizes with Mpc1p to form the respiratory isoform of MPC; MPC localizes to the mitochondrial inner membrane and mediates pyruvate uptake; MPC3 paralog, MPC2, heterodimerizes with Mpc1p to form the fermentative MPC isoform; protein abundance increases in response to DNA replication stress
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0.05
Bud 0.06 0 0.06 0.06 0.05 0 – 0 0.05 0.05 0.06 0.05 0 0 0 0 0 0 0 0 0 0 0 0.05
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.23 0.38 0.37 0.17 0.11 0.07 – 0.05 0 0.05 0 0.05 0.09 0.11 0.12 0.44 0.5 0.45 0.07 0.06 0.05 0.06 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0.05 0.05 0.06 0 0 0 0 0 0
Endosome 0 0.07 0 0 0.08 0 – 0 0 0 0 0 0.14 0.14 0.17 0.12 0.15 0.14 0.08 0 0.09 0.06 0.06 0.07
Golgi 0 0 0 0 0 0 – 0 0 0 0 0 0 0.07 0.05 0 0 0 0 0 0 0 0 0
Mitochondria 0.7 0.51 0.66 0.9 0.77 0.9 – 0.91 0.94 0.93 0.97 0.95 0.73 0.63 0.56 0.12 0.12 0.13 0.62 0.7 0.63 0.63 0.63 0.57
Nucleus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0.09 0 – 0 0 0 0 0 0.11 0.11 0.16 0 0 0.08 0 0 0 0 0 0
SpindlePole 0 0 0.07 0 0.05 0 – 0.05 0 0 0 0 0 0 0.05 0.09 0.12 0.13 0 0 0 0 0 0
Vac/Vac Membrane 0 0.07 0 0 0.05 0 – 0 0 0 0 0 0.13 0.1 0.15 0.2 0.14 0.09 0.09 0.07 0 0.07 0.07 0.06
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 6 2 1 0 0 0 – 0 0 0 0 1 0 3 0 8 5 1 5 3 23 4 13 21
Bud 10 5 32 25 25 25 – 29 44 46 46 41 3 5 4 5 2 10 1 2 5 6 9 21
Bud Neck 3 0 9 0 0 0 – 1 0 0 0 0 0 0 0 1 2 2 1 1 8 7 5 11
Bud Site 0 0 4 3 3 9 – 15 9 17 17 28 0 0 0 0 0 2 – – – – – –
Cell Periphery 6 3 5 7 4 1 – 4 6 14 4 13 13 21 12 4 1 10 0 0 3 0 2 3
Cytoplasm 42 56 214 70 54 50 – 53 35 44 22 39 35 50 51 103 54 106 17 10 31 19 13 11
Endoplasmic Reticulum 3 3 7 2 5 7 – 2 7 3 3 1 6 10 1 12 5 13 2 2 7 3 2 4
Endosome 4 11 2 0 41 13 – 18 1 3 0 0 56 65 75 27 16 32 20 5 59 20 21 27
Golgi 0 0 0 0 1 0 – 0 0 0 0 0 9 34 22 8 1 8 0 3 6 8 6 18
Mitochondria 127 76 375 374 379 634 – 924 781 831 705 725 289 292 247 29 13 30 156 130 401 207 231 235
Nucleus 4 2 1 1 6 8 – 24 12 9 4 9 2 1 0 5 2 4 1 1 0 0 0 1
Nuclear Periphery 1 1 0 0 2 9 – 13 16 12 16 16 4 5 1 0 0 1 1 1 3 1 1 1
Nucleolus 2 0 1 1 6 13 – 21 11 10 8 14 4 2 2 0 1 1 0 0 0 2 1 2
Peroxisomes 0 3 9 9 42 18 – 18 7 15 2 5 44 50 72 8 3 19 3 1 25 7 10 7
SpindlePole 4 2 39 10 24 24 – 47 31 22 25 26 8 3 20 20 13 31 8 2 20 10 12 10
Vac/Vac Membrane 5 10 13 5 24 14 – 43 24 19 15 19 53 46 68 46 15 22 23 13 19 23 25 25
Unique Cell Count 181 149 572 417 491 706 1011 827 889 729 761 398 461 443 234 107 236 254 185 634 331 367 411
Labelled Cell Count 217 174 712 507 616 825 1212 984 1045 867 937 526 587 575 276 133 292 254 185 634 331 367 411


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.5 4.8 3.5 3.5 4.4 3.6 – 3.7 3.5 3.8 3.7 3.5 6.5 6.5 6.5 5.6 5.8 5.8 5.3 5.8 6.8
Std Deviation (1e-4) 0.4 1.4 1.0 1.5 1.6 1.3 – 1.1 1.3 1.2 1.1 1.1 4.9 1.6 1.8 1.5 1.7 2.0 2.6 3.2 5.3
Intensity Change (Log2) – – – 0.02 0.34 0.07 – 0.08 -0.01 0.12 0.07 -0.01 0.9 0.9 0.89 0.7 0.74 0.74 0.61 0.73 0.96


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – 0 – 0
Bud Neck – – – – – – – – – – – – 0 – 0
Bud Site – – – – – – – – – – – – 0 – 0
Cell Periphery – – – – – – – – – – – – 0 – 0
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – 0 – 0
Endosome – – – – – – – – – – – – 0 – 0
Golgi – – – – – – – – – – – – 0 – 0
Mitochondria – – – – – – – – – – – – 0 – 0
Nuclear Periphery – – – – – – – – – – – – 0 – 0
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – 0 – 0
Peroxisomes – – – – – – – – – – – – 0 – 0
SpindlePole – – – – – – – – – – – – 0 – 0
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – 0 – 0
Cytoplasm – – – – – – – – – – – – 0 – 0
Nucleus – – – – – – – – – – – – 0 – 0
Vacuole – – – – – – – – – – – – 8.6 – 4.5

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 6.4695 7.1878 6.3041 5.9123 6.0289 6.7495 5.5118 6.9132 6.6088 4.4261 4.1706 5.2139 7.1661 7.9 8.3167 7.0248 7.4185 7.4235
Actin 0.0046 0.001 0.0109 0.0005 0.0081 0.0022 0.0117 0.0029 0.0123 0.0007 0.0017 0.0011 0.0013 0.0157 0.0014 0.0002 0.0005 0.0002
Bud 0.0002 0.0001 0.0011 0 0.0004 0.0001 0.0005 0.0003 0.0006 0.0003 0.0004 0.0001 0.0003 0.0007 0.0003 0.0047 0.0001 0
Bud Neck 0.0003 0.0001 0.0007 0.0001 0.0009 0.0001 0.0005 0.0001 0.0002 0 0 0.0001 0.0001 0.0037 0.0013 0 0 0
Bud Periphery 0.0005 0.0003 0.0016 0.0001 0.001 0.0003 0.001 0.0006 0.0013 0.0011 0.0017 0.0002 0.0008 0.0011 0.0008 0.0259 0.0003 0.0001
Bud Site 0.0006 0.0001 0.0023 0.0001 0.0106 0.0001 0.001 0.0004 0.0017 0.0002 0.0002 0.0003 0.001 0.005 0.001 0.0001 0.0001 0
Cell Periphery 0.0003 0.0003 0.0003 0.0001 0.0005 0.0001 0.0004 0.0002 0.0002 0.0001 0.0001 0.0001 0.0003 0.0005 0.0015 0.0002 0.0001 0.0001
Cytoplasm 0.0015 0.0001 0.0005 0.0001 0.0007 0.0001 0.0012 0.0001 0.0001 0 0.0005 0 0.0001 0.0001 0.0001 0.0001 0 0
Cytoplasmic Foci 0.0076 0.0022 0.0052 0.0134 0.0017 0.0015 0.0109 0.0033 0.0025 0.0005 0.0023 0.0014 0.0027 0.0045 0.0011 0.0002 0.0011 0.0006
Eisosomes 0.0003 0.0001 0.0002 0.0001 0.0004 0.0001 0.0006 0.0001 0.0003 0.0001 0.0001 0.0001 0.0002 0.0002 0.0005 0.0001 0 0.0001
Endoplasmic Reticulum 0.001 0.0001 0.0002 0 0.0012 0.0002 0.0018 0.0001 0.0002 0.0001 0.0003 0 0.0002 0.0001 0.0002 0.0001 0 0.0001
Endosome 0.0197 0.0116 0.0085 0.0158 0.0072 0.0086 0.0353 0.0165 0.004 0.0032 0.0095 0.0041 0.0221 0.0174 0.0098 0.0035 0.0027 0.0081
Golgi 0.0333 0.0303 0.0153 0.009 0.0263 0.0236 0.0237 0.0195 0.0069 0.0025 0.0043 0.0099 0.0282 0.0208 0.0114 0.0031 0.0038 0.0199
Lipid Particles 0.0116 0.0059 0.0028 0.0071 0.0017 0.0024 0.009 0.0035 0.0041 0.0006 0.0484 0.0088 0.0211 0.011 0.0051 0.0005 0.0012 0.0051
Mitochondria 0.8936 0.9324 0.9318 0.9185 0.93 0.9496 0.8677 0.9353 0.9574 0.9864 0.8997 0.968 0.88 0.8798 0.9394 0.9574 0.9783 0.9588
None 0.0019 0 0.0039 0 0.0011 0.0001 0.0028 0 0.0001 0 0.0035 0 0.0005 0.0001 0.0001 0.0001 0 0
Nuclear Periphery 0.0009 0.0001 0.0005 0 0.0011 0.0016 0.0034 0.0001 0.0001 0 0.0071 0 0.0017 0.0001 0.0001 0.0001 0 0.0002
Nucleolus 0.0003 0.0001 0.0006 0.0001 0.0005 0.0001 0.0007 0.0002 0.0003 0.0001 0.0021 0.0002 0.0102 0.0013 0.0004 0 0 0.0001
Nucleus 0.0003 0.0001 0.0005 0 0.0009 0.0002 0.001 0.0001 0.0001 0.0001 0.0015 0.0001 0.0046 0.0002 0.0002 0.0001 0 0.0001
Peroxisomes 0.007 0.0018 0.0054 0.0269 0.0013 0.0015 0.0108 0.005 0.0053 0.0015 0.0044 0.0034 0.0052 0.0075 0.0023 0.0003 0.0076 0.0011
Punctate Nuclear 0.0004 0 0.0013 0.0002 0.0005 0.0001 0.0011 0 0.0001 0 0.0059 0 0.0012 0 0 0 0 0
Vacuole 0.0054 0.004 0.0031 0.002 0.0013 0.0011 0.0062 0.0037 0.0007 0.0011 0.0017 0.0004 0.0058 0.0107 0.0093 0.0012 0.0008 0.0011
Vacuole Periphery 0.0088 0.0092 0.0031 0.0057 0.0026 0.0064 0.0086 0.0078 0.0015 0.0013 0.0045 0.0016 0.0125 0.0196 0.0137 0.0024 0.0033 0.0041

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 5.0041 6.71 3.3194 7.8676 6.488 20.0053 10.1768 7.6318 12.3496 6.7841
Translational Efficiency 2.2941 0.7184 1.9061 1.2091 1.0563 1.1553 1.0419 2.2035 1.3143 1.2379

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
74 218 92 19 365 249 2474 8 439 467 2566 27

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 653.40 650.67 875.26 760.71 918.85 692.85 786.71 329.22 874.10 673.16 789.88 632.86
Standard Deviation 72.58 88.24 117.23 112.13 163.85 77.53 106.14 4.67 181.89 85.33 107.82 218.34
Intensity Change Log 2 — -0.006040 0.421745 0.219380 — -0.407286 -0.223997 -1.480777 — -0.226813 0.080064 -0.528595

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.002837 0.000364 0.006537 0.000585 0.003942 0.002374 0.001688 0.000115 0.003756 0.001436 0.001862 0.000446
Bud Neck 0.030728 0.033750 0.034347 0.120864 0.002928 0.045383 0.096706 0.000005 0.007614 0.039953 0.094470 0.085054
Bud Site 0.015162 0.019025 0.104430* 0.098165 0.005340 0.022554 0.117319* 0.000016 0.006995 0.020907 0.116857* 0.069084
Cell Periphery 0.000865 0.000633 0.000272 0.000504 0.003404 0.000672 0.000320 0.000247 0.002976 0.000654 0.000319 0.000428
Cytoplasm 0.167751* 0.022239 0.072227 0.087816 0.002845 0.104221* 0.065890 0.000025 0.030642 0.065951 0.066117 0.061804
Cytoplasmic Foci 0.305259* 0.118497 0.139291 0.076464 0.006675 0.295439* 0.040330 0.000011 0.057006 0.212841* 0.043878 0.053811
Eisosomes 0.000559 0.000136 0.000132 0.000145 0.000074 0.000450 0.000084 0.001429 0.000156 0.000303 0.000085 0.000526
Endoplasmic Reticulum 0.003561 0.001011 0.006841 0.000477 0.000735 0.001393 0.001966 0.000001 0.001212 0.001215 0.002141 0.000336
Endosome 0.033069 0.093802 0.067028 0.167607 0.005006 0.052699 0.067335 0.000000 0.009736 0.071886 0.067324 0.117946
Golgi 0.013458 0.097997 0.045847 0.079940 0.010870 0.034495 0.025632 0.000003 0.011306 0.064138 0.026356 0.056255
Lipid Particles 0.030488 0.011938 0.004006 0.004667 0.001278 0.019367 0.001150 0.000030 0.006202 0.015899 0.001252 0.003293
Mitochondria 0.092474 0.430305* 0.092251 0.084788 0.936141* 0.161360* 0.275270* 0.000012 0.793929* 0.286906* 0.268709* 0.059669
Mitotic Spindle 0.007303 0.019668 0.154395* 0.108545* 0.002430 0.008036 0.085458 0.000163 0.003251 0.013466 0.087929 0.076432
None 0.002293 0.003201 0.000825 0.001221 0.000648 0.000650 0.000892 0.997751* 0.000925 0.001841 0.000889 0.296490*
Nuclear Periphery 0.000516 0.000194 0.000918 0.000169 0.000090 0.000497 0.000623 0.000000 0.000162 0.000355 0.000633 0.000119
Nuclear Periphery Foci 0.000623 0.000750 0.002132 0.000340 0.000311 0.000276 0.000334 0.000001 0.000363 0.000497 0.000398 0.000239
Nucleolus 0.003485 0.001152 0.003566 0.010950 0.000580 0.001674 0.002162 0.000002 0.001069 0.001431 0.002212 0.007706
Nucleus 0.149581* 0.047397 0.040177 0.022122 0.003783 0.048278 0.029682 0.000004 0.028359 0.047867 0.030059 0.015568
Peroxisomes 0.103901* 0.067693 0.095145 0.064740 0.003399 0.173225* 0.031452 0.000184 0.020340 0.123962* 0.033735 0.045612
Vacuole 0.033167 0.014854 0.112316 0.058380 0.004191 0.022987 0.137927 0.000000 0.009075 0.019190 0.137009 0.041082
Vacuole Periphery 0.002920 0.015392 0.017316 0.011511 0.005331 0.003972 0.017782 0.000000 0.004924 0.009303 0.017765 0.008100

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 1.76 -1.38 1.60 -1.30 2.79 NaN NaN NaN NaN NaN 1.47 1.23 2.20 1.88 5.63
Bud Neck -0.29 -0.51 -1.75 -1.70 -1.66 NaN NaN NaN NaN NaN -5.95 -23.51 -2.09 -1.20 0.28
Bud Site -0.61 -5.08 -2.41 -2.30 0.07 NaN NaN NaN NaN NaN -3.81 -28.73 -2.44 -1.88 1.80
Cell Periphery 0.44 1.51 0.80 0.30 -1.13 NaN NaN NaN NaN NaN 1.75 2.02 1.92 0.98 -0.82
Cytoplasm 5.81 2.98 1.50 -1.37 -0.18 NaN NaN NaN NaN NaN -4.44 -6.89 -0.94 0.14 0.27
Cytoplasmic Foci 6.50 5.57 7.08 2.00 2.24 NaN NaN NaN NaN NaN -12.58 1.87 0.23 9.27 -0.74
Eisosomes 3.70 3.76 3.40 -0.32 -0.36 NaN NaN NaN NaN NaN -3.58 3.01 -1.04 -0.63 -1.23
Endoplasmic Reticulum 1.42 -1.91 1.79 1.86 4.70 NaN NaN NaN NaN NaN -0.09 -3.30 2.59 3.62 12.54
Endosome -4.65 -2.54 -4.00 -2.17 -3.11 NaN NaN NaN NaN NaN -10.01 -19.86 -3.95 -1.64 -1.92
Golgi -7.64 -3.02 -1.90 0.49 -1.06 NaN NaN NaN NaN NaN -8.67 -6.14 -1.76 0.30 -1.22
Lipid Particles 2.20 3.89 3.54 1.16 -0.35 NaN NaN NaN NaN NaN -3.01 3.45 1.17 3.61 -1.17
Mitochondria -9.78 0.21 0.10 6.35 -0.02 NaN NaN NaN NaN NaN 21.34* 29.01 19.39 5.99 5.77
Mitotic Spindle -2.13 -5.66 -2.00 -1.70 0.65 NaN NaN NaN NaN NaN -3.53 -25.38 -2.00 -1.68 0.33
None -0.48 1.86 1.18 1.02 -0.31 NaN NaN NaN NaN NaN -1.07 0.13 -3.31 -3.30 -3.31
Nuclear Periphery 3.11 -2.64 2.51 0.25 3.95 NaN NaN NaN NaN NaN -1.59 -9.39 0.42 1.61 6.63
Nuclear Periphery Foci -0.26 -2.73 1.74 0.87 3.28 NaN NaN NaN NaN NaN -0.56 -0.67 1.08 1.14 2.06
Nucleolus 2.10 -0.03 -1.14 -1.50 -1.13 NaN NaN NaN NaN NaN -0.86 -3.29 -1.42 -1.34 -1.15
Nucleus 3.29 2.61 4.04 1.32 1.81 NaN NaN NaN NaN NaN -2.58 -1.45 0.77 2.30 1.45
Peroxisomes 1.84 0.58 1.68 0.11 1.00 NaN NaN NaN NaN NaN -10.25 -3.25 -2.07 5.12 -1.05
Vacuole 3.05 -4.51 -1.07 -1.90 2.20 NaN NaN NaN NaN NaN -4.27 -33.98 -1.92 -1.29 6.19
Vacuole Periphery -3.39 -3.19 -1.77 0.62 0.68 NaN NaN NaN NaN NaN -2.17 -10.38 -0.85 0.29 2.49
Standard name
Human Ortholog
Description Highly conserved subunit of the mitochondrial pyruvate carrier (MPC); expressed during growth on nonfermentable carbon sources, and heterodimerizes with Mpc1p to form the respiratory isoform of MPC; MPC localizes to the mitochondrial inner membrane and mediates pyruvate uptake; MPC3 paralog, MPC2, heterodimerizes with Mpc1p to form the fermentative MPC isoform; protein abundance increases in response to DNA replication stress
Localization
Cell Percentages mitochondrion (87%)
Cell Cycle Regulation No
Subcompartmental Group mito-2

Mpc3

Mpc3


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Mpc3-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available