Standard name
Human Ortholog
Description Flavin-dependent monooxygenase involved in ubiquinone biosynthesis; responsible for hydroxylation at position C5 and deamination at C4 during ubiquinone (Coenzyme Q) biosynthesis; localizes to matrix face of mitochondrial inner membrane in a large complex with other ubiquinone biosynthetic enzymes; human homolog COQ6 can complement yeast null mutant and is implicated in steroid-resistant nephrotic syndrome (SRNS)
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0.06 0.09 0.06 0.07 0 0.07 0.09 0.11 0.08 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.08 0.14 0.1 0 0.08 0.05 0.06 0 0.05 0 0 0 0.17 0.18 0.17 0.09 0.06 0.1 0 0 0 0.08 0.05 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0.1 0.17 0 0 0.08 0 0 0 0 0 0 0 0.31 0.31 0.31 0.2 0.08 0.16 0.23 0.19 0.25 0.16 0.21 0.22
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0.27 0.08 0 0 0 0 0 0
Mitochondria 0.61 0.45 0.56 0.75 0.66 0.79 0.84 0.82 0.85 0.9 0.92 0.95 0.27 0.32 0.24 0.1 0.14 0.07 0.25 0.28 0.25 0.17 0.15 0.17
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0.06 0.09 0 0 0.05 0.05 0.08 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0.09 0 0 0 0.07 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0.18 0.29 0.23 0.15 0.22 0.13 0.06 0.09 0 0 0.05 0 0.46 0.42 0.5 0.52 0.32 0.46 0.22 0.15 0.3 0.24 0.22 0.24
SpindlePole 0.2 0.28 0.29 0.24 0.18 0.11 0.12 0.15 0.1 0.08 0 0 0.24 0.23 0.15 0.32 0.29 0.32 0.18 0.21 0.12 0.23 0.25 0.19
Vac/Vac Membrane 0 0.07 0 0 0 0 0 0 0 0 0 0 0.06 0 0.05 0 0 0.05 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 1 2 1 0 0 0 0 0 1 0 1 1 1 0 0 3 0 3 4 15 1 7 8 15
Bud 7 28 11 4 8 6 12 23 19 14 8 7 19 20 7 12 6 13 1 15 0 4 11 14
Bud Neck 5 7 2 1 1 0 1 1 3 1 0 0 4 2 1 0 0 0 1 6 0 5 3 9
Bud Site 4 10 2 0 1 3 4 4 4 2 3 1 5 0 0 2 0 6 – – – – – –
Cell Periphery 3 11 1 0 1 0 0 0 1 2 0 2 12 6 4 10 3 9 0 0 0 0 1 0
Cytoplasm 21 72 12 3 9 9 10 11 9 5 8 4 99 106 112 47 16 28 3 17 0 28 19 14
Endoplasmic Reticulum 1 0 0 2 0 0 2 0 1 1 1 1 1 0 1 8 5 3 3 0 0 1 1 3
Endosome 25 84 1 1 9 7 5 4 1 1 0 0 184 181 201 101 20 47 65 104 31 58 88 115
Golgi 1 0 1 0 0 0 0 0 0 0 0 1 4 3 11 42 68 24 6 16 2 9 9 11
Mitochondria 154 227 69 51 76 138 145 221 142 154 177 148 159 188 153 48 36 21 71 152 31 64 62 91
Nucleus 3 0 1 0 0 1 1 1 1 1 1 0 1 1 1 5 2 1 0 0 1 0 0 0
Nuclear Periphery 2 4 0 1 1 11 15 9 4 8 10 12 0 1 1 2 4 0 1 0 0 2 1 2
Nucleolus 4 7 1 0 4 15 7 7 7 12 7 8 3 4 4 13 3 11 2 2 0 2 4 3
Peroxisomes 46 144 28 10 25 23 10 23 3 5 9 3 275 248 320 255 82 136 64 82 38 88 94 128
SpindlePole 50 142 36 16 21 19 21 39 17 13 7 5 146 132 98 156 74 94 50 115 15 87 106 98
Vac/Vac Membrane 11 34 1 1 3 1 0 6 1 0 1 2 33 20 34 17 11 14 3 8 0 5 6 9
Unique Cell Count 254 500 123 68 115 175 173 268 168 172 192 156 597 584 641 495 256 294 286 552 129 374 427 528
Labelled Cell Count 338 772 167 90 159 233 233 349 214 219 233 195 946 912 948 721 330 410 286 552 129 374 427 528


Mitochondria, Punctate Nuclear

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.3 4.9 4.2 3.7 4.6 4.5 4.3 4.8 4.8 5.0 5.3 5.4 5.8 5.7 6.0 8.0 8.9 8.5 4.6 4.6 5.6
Std Deviation (1e-4) 0.9 1.1 1.4 1.0 1.2 1.3 1.2 1.5 1.5 1.6 1.6 1.6 1.4 1.5 1.8 4.6 2.0 1.8 1.4 1.4 1.5
Intensity Change (Log2) – – – -0.19 0.11 0.08 0.02 0.18 0.17 0.25 0.31 0.36 0.45 0.42 0.49 0.92 1.06 1.0 0.11 0.12 0.39


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 4.5978 4.0775 4.5044 4.2622 4.2841 4.2958 4.9985 3.9614 3.965 3.1972 4.0037 3.8381 5.807 4.8537 5.3568 5.9309 4.6089 5.2957
Actin 0.0414 0.0152 0.0093 0.0052 0.0021 0.0103 0.0886 0.0423 0.0128 0.0059 0.0355 0.0177 0.018 0.0286 0.0238 0.0072 0.0871 0.0262
Bud 0.0011 0.0061 0.0009 0.0007 0.0222 0.0001 0.0004 0.0022 0.0012 0.0008 0.0005 0.0005 0.0009 0.005 0.0028 0.0002 0.0008 0.0005
Bud Neck 0.0018 0.0019 0.0005 0.0011 0.0013 0.0007 0.001 0.0004 0.0111 0.0004 0.0012 0.0103 0.0035 0.0039 0.0065 0.0062 0.0008 0.0014
Bud Periphery 0.0018 0.0032 0.0018 0.0007 0.0475 0.0002 0.0011 0.0021 0.0032 0.0013 0.0008 0.0011 0.0013 0.0049 0.0044 0.0003 0.0011 0.0005
Bud Site 0.0052 0.0319 0.0057 0.0041 0.0205 0.0004 0.0019 0.0072 0.0055 0.0015 0.0016 0.0006 0.0049 0.0566 0.0185 0.006 0.0022 0.0008
Cell Periphery 0.0003 0.0005 0.0001 0.0002 0.0007 0.0001 0.0005 0.0001 0.0002 0.0001 0.0001 0.0002 0.0003 0.0004 0.0004 0.0001 0.0004 0.0001
Cytoplasm 0.0023 0.0092 0.0016 0.0049 0.006 0.0013 0.0193 0.0024 0.0046 0.0071 0.0015 0.0039 0.0017 0.007 0.0061 0.0003 0.0095 0.0078
Cytoplasmic Foci 0.0815 0.1863 0.0849 0.2217 0.0683 0.1042 0.179 0.1981 0.1488 0.2587 0.1785 0.1634 0.1594 0.218 0.1667 0.1572 0.1365 0.1837
Eisosomes 0.0006 0.0002 0.0002 0.0001 0.0009 0.0002 0.0008 0.0002 0.0002 0.0001 0.0011 0.0001 0.0001 0.0003 0.0002 0.0001 0.0003 0.0001
Endoplasmic Reticulum 0.0009 0.0009 0.0004 0.0002 0.0008 0 0.0063 0.0006 0.0008 0.0006 0.0007 0.0017 0.0005 0.001 0.0014 0.0001 0.0012 0.0004
Endosome 0.0641 0.0726 0.042 0.0553 0.0375 0.0115 0.0534 0.0359 0.0176 0.0523 0.0184 0.0259 0.066 0.0506 0.044 0.0381 0.0582 0.0327
Golgi 0.0564 0.0464 0.034 0.0682 0.0466 0.0241 0.0295 0.0418 0.0263 0.0627 0.0291 0.0422 0.0483 0.053 0.0671 0.0445 0.0424 0.0516
Lipid Particles 0.1653 0.0639 0.1183 0.0662 0.0606 0.1316 0.0834 0.0779 0.0409 0.061 0.1283 0.0791 0.2014 0.0609 0.0887 0.0787 0.1603 0.1519
Mitochondria 0.0795 0.0384 0.1151 0.1718 0.0391 0.1962 0.0601 0.055 0.0927 0.0271 0.0219 0.081 0.0613 0.1053 0.0859 0.1687 0.0427 0.0897
None 0.0026 0.0602 0.0351 0.0011 0.1373 0.054 0.0362 0.0427 0.0373 0.0529 0.0517 0.015 0.0012 0.0103 0.0245 0.0001 0.0712 0.0094
Nuclear Periphery 0.0009 0.0009 0.0004 0.0001 0.0002 0.0001 0.0181 0.0004 0.0004 0.0018 0.0015 0.0032 0.0029 0.0014 0.0011 0.0001 0.0006 0.0002
Nucleolus 0.0009 0.0015 0.0006 0.0002 0.0022 0.0002 0.0005 0.0003 0.001 0.0006 0.0012 0.0016 0.0037 0.0095 0.0015 0.0004 0.001 0.003
Nucleus 0.0005 0.0015 0.0003 0.0001 0.0007 0.0001 0.0019 0.0002 0.0006 0.0006 0.0006 0.0049 0.0017 0.0009 0.0006 0.0001 0.0009 0.0003
Peroxisomes 0.4856 0.4093 0.5455 0.3838 0.5 0.4601 0.4029 0.4816 0.5414 0.4591 0.5187 0.5177 0.4017 0.3538 0.4454 0.4452 0.3773 0.4239
Punctate Nuclear 0.0013 0.0459 0.0019 0.0123 0.0012 0.0036 0.013 0.0072 0.052 0.0028 0.0062 0.0274 0.0025 0.0122 0.0062 0.0451 0.0018 0.0127
Vacuole 0.0037 0.0028 0.0008 0.0014 0.0037 0.0004 0.0011 0.0009 0.0009 0.0016 0.0004 0.0012 0.0104 0.0102 0.003 0.0006 0.0027 0.0016
Vacuole Periphery 0.0021 0.0011 0.0006 0.0007 0.0006 0.0007 0.0011 0.0004 0.0004 0.001 0.0003 0.0011 0.0083 0.0063 0.0014 0.0006 0.0011 0.0013

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 28.3514 11.6447 18.8066 28.1216 22.8501 19.9116 23.3748 32.4877 28.7925 30.3497
Translational Efficiency 0.9695 1.1069 0.7682 0.5359 0.6272 1.4037 0.725 0.4904 0.7251 0.7179

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Flavin-dependent monooxygenase involved in ubiquinone biosynthesis; responsible for hydroxylation at position C5 and deamination at C4 during ubiquinone (Coenzyme Q) biosynthesis; localizes to matrix face of mitochondrial inner membrane in a large complex with other ubiquinone biosynthetic enzymes; human homolog COQ6 can complement yeast null mutant and is implicated in steroid-resistant nephrotic syndrome (SRNS)
Localization
Cell Percentages mitochondrion (34%), peroxisome (14%), mixed (13%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Coq6

Coq6


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Coq6-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available