Standard name
Human Ortholog
Description Single-stranded DNA endonuclease; cleaves single-stranded DNA during nucleotide excision repair to excise damaged DNA; subunit of Nucleotide Excision Repair Factor 3 (NEF3); homolog of human XPG protein
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0.12 0.07 0.1 0.08 0.1 0.12 0.11 0.09 0.06 0.05 0.08 0.16 0.26 0.27 0.07 0.1 0.11 0 0 0 0.06 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.18 0.39 0.57 0.61 0.29 0.59 0.59 0.62 0.81 0.77 0.79 0.84 0 0 0.09 0 0 0.05 0 0 0 0 0 0
Nucleus 0.84 0.68 0.7 0.7 0.75 0.56 0.59 0.55 0.51 0.5 0.48 0.43 0.67 0.57 0.52 0.83 0.78 0.76 0.82 0.74 0.61 0.71 0.67 0.54
Nuclear Periphery 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0
Vac/Vac Membrane 0.12 0.2 0 0 0.06 0.08 0 0 0 0 0 0.07 0.19 0.21 0.18 0.1 0.14 0.15 0.1 0.07 0.31 0.13 0.18 0.25
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 1 2
Bud 0 0 0 2 1 2 2 7 6 4 5 4 0 0 6 0 1 1 2 2 1 1 12 12
Bud Neck 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 1 0 0 0 0 0 2 1 4
Bud Site 0 0 0 1 2 3 6 13 14 14 5 9 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0
Cytoplasm 4 5 7 26 30 36 65 67 34 33 12 39 45 133 170 45 37 93 2 1 4 9 17 21
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 1 0 0 5 2 18 5 43 0 0 0 1 1 2
Endosome 0 0 0 0 2 0 2 0 0 0 0 0 0 4 8 10 4 19 2 1 0 0 2 16
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 2 2 0 3 0 0 0 2 2
Mitochondria 38 16 55 164 108 213 334 392 303 403 196 421 12 7 56 20 7 41 3 1 0 0 4 17
Nucleus 179 28 67 188 277 202 335 349 190 264 119 214 190 296 322 528 274 665 184 52 75 101 295 365
Nuclear Periphery 1 2 0 0 1 0 0 0 0 2 0 0 0 0 2 1 0 3 0 0 0 0 0 2
Nucleolus 1 0 0 0 1 2 0 2 0 3 4 1 1 0 1 0 0 0 0 1 0 1 4 16
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0
SpindlePole 0 0 0 3 0 1 3 8 2 1 0 1 1 0 0 2 0 5 2 3 0 2 9 26
Vac/Vac Membrane 26 8 2 4 23 29 24 25 6 21 6 33 53 109 113 66 48 129 23 4 38 18 80 168
Unique Cell Count 214 41 96 270 371 362 564 631 375 525 247 499 283 517 623 636 353 871 226 70 124 142 441 673
Labelled Cell Count 249 59 131 388 445 488 771 865 555 745 348 722 302 556 682 693 378 999 226 70 124 142 441 673


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.9 4.1 3.6 3.7 3.9 3.6 3.4 3.4 3.1 3.1 3.2 2.9 5.6 5.4 4.8 6.6 7.1 7.3 5.1 5.3 5.4
Std Deviation (1e-4) 0.7 0.8 0.5 1.0 1.0 0.9 0.8 0.8 1.1 0.8 1.1 0.8 1.2 1.2 1.0 1.3 1.3 1.3 1.1 1.4 1.1
Intensity Change (Log2) – – – 0.04 0.13 -0.02 -0.09 -0.1 -0.21 -0.22 -0.16 -0.33 0.62 0.58 0.42 0.88 0.98 1.03 0.51 0.55 0.57


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – 0 0 0
Bud Neck – – – – – – – – – – – – 0 0 0
Bud Site – – – – – – – – – – – – 0 0 0
Cell Periphery – – – – – – – – – – – – 0 0 0
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – 0 0 0
Endosome – – – – – – – – – – – – 0 0 0
Golgi – – – – – – – – – – – – 0 0 0
Mitochondria – – – – – – – – – – – – 0 0 0
Nuclear Periphery – – – – – – – – – – – – 0 0 0
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – 0 0 0
Peroxisomes – – – – – – – – – – – – 0 0 0
SpindlePole – – – – – – – – – – – – 0 0 0
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – 0 0 0
Cytoplasm – – – – – – – – – – – – 0 0 0
Nucleus – – – – – – – – – – – – 0 0 0
Vacuole – – – – – – – – – – – – 2.6 3.2 3.5

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 1.2529 0.8847 1.0645 0.4755 0.5816 0.7649 1.4862 1.2914 1.2409 0.9597 0.615 0.9195 -0.3104 0.1377 -0.1853 0.1571 -0.2703 -0.3091
Actin 0.0943 0.0002 0.0205 0.0097 0.0044 0.0007 0.0471 0.0001 0.019 0.0933 0.0019 0.0051 0.0226 0.0002 0.0094 0.0124 0.0002 0.0008
Bud 0.0008 0.0003 0.0006 0.0004 0.0002 0.0005 0.0012 0.0001 0.0004 0.0019 0.0018 0.0002 0.0007 0.0001 0.0003 0.0001 0 0.0001
Bud Neck 0.0055 0.0007 0.0028 0.0052 0.001 0.0055 0.0097 0.0005 0.0061 0.0072 0.0007 0.0017 0.0019 0.0005 0.002 0.0006 0.0004 0.0028
Bud Periphery 0.0019 0.0001 0.002 0.0005 0.0005 0.0013 0.0016 0 0.0007 0.0044 0.0023 0.0004 0.001 0.0001 0.0006 0.0001 0 0.0001
Bud Site 0.0081 0.0021 0.0085 0.001 0.0002 0.0034 0.0236 0.0007 0.0022 0.0138 0.0097 0.0004 0.0025 0.0008 0.0036 0.0003 0.0001 0.0002
Cell Periphery 0.0006 0.0001 0.0005 0.0001 0.0001 0.0002 0.0006 0 0.0001 0.0023 0.0001 0 0.0002 0.0001 0.0004 0 0 0
Cytoplasm 0.0382 0.1381 0.0451 0.0914 0.0717 0.0535 0.0566 0.0987 0.0456 0.0294 0.0164 0.0383 0.0551 0.0715 0.0561 0.0646 0.0434 0.0489
Cytoplasmic Foci 0.0141 0.0035 0.0116 0.0137 0.0027 0.0021 0.0279 0.0012 0.0042 0.0062 0.0068 0.0028 0.0097 0.0028 0.0029 0.0077 0.001 0.0023
Eisosomes 0.0008 0 0.0007 0.0001 0.0001 0 0.0003 0 0.0001 0.0006 0 0 0.0004 0 0.0004 0.0001 0 0
Endoplasmic Reticulum 0.008 0.0076 0.0031 0.0034 0.003 0.0024 0.0056 0.0037 0.0023 0.0023 0.0003 0.0014 0.0029 0.0029 0.0027 0.0023 0.0013 0.0012
Endosome 0.0251 0.0027 0.0079 0.0087 0.0145 0.0034 0.0413 0.0013 0.0063 0.0168 0.004 0.0037 0.0057 0.0031 0.003 0.0104 0.001 0.0021
Golgi 0.0086 0.0001 0.0031 0.0036 0.0045 0.0006 0.0105 0 0.0084 0.0143 0.0037 0.0023 0.0029 0.0001 0.0006 0.0031 0 0.0006
Lipid Particles 0.0212 0.0004 0.0085 0.0037 0.0241 0.0083 0.0253 0.0001 0.0095 0.0047 0.0037 0.0035 0.0064 0.0005 0.0017 0.0139 0.0001 0.0041
Mitochondria 0.0093 0.0004 0.0171 0.0039 0.008 0.0008 0.0063 0.0002 0.0026 0.0172 0.0109 0.009 0.0042 0.0005 0.0066 0.0112 0.0004 0.0092
None 0.0373 0.0675 0.0299 0.0537 0.0456 0.0263 0.0371 0.0502 0.043 0.0352 0.0044 0.0203 0.1275 0.1559 0.1227 0.1589 0.2213 0.0931
Nuclear Periphery 0.0545 0.0271 0.0285 0.0195 0.0451 0.0199 0.0274 0.0149 0.0123 0.0097 0.005 0.01 0.0179 0.0209 0.0181 0.0178 0.0275 0.0095
Nucleolus 0.0056 0.0066 0.007 0.0035 0.0043 0.0112 0.0044 0.0027 0.0042 0.0048 0.0032 0.0029 0.0036 0.0045 0.0059 0.003 0.0029 0.0074
Nucleus 0.6259 0.7243 0.7727 0.7185 0.7524 0.8133 0.6073 0.8088 0.8101 0.7176 0.9098 0.878 0.6891 0.7145 0.7398 0.6381 0.6928 0.7788
Peroxisomes 0.0126 0.0001 0.0072 0.0059 0.001 0.0021 0.0092 0 0.002 0.0076 0.0079 0.0055 0.0096 0.0001 0.0021 0.0295 0 0.0015
Punctate Nuclear 0.0153 0.0152 0.0174 0.051 0.0111 0.041 0.0488 0.015 0.0194 0.0038 0.0057 0.0133 0.0341 0.019 0.0187 0.0248 0.0064 0.0361
Vacuole 0.0085 0.0025 0.004 0.002 0.0022 0.0032 0.0056 0.0013 0.001 0.0052 0.0014 0.0008 0.0015 0.0018 0.002 0.0006 0.0009 0.0008
Vacuole Periphery 0.0038 0.0004 0.0013 0.0006 0.0031 0.0004 0.0025 0.0002 0.0005 0.0014 0.0003 0.0003 0.0004 0.0003 0.0004 0.0004 0.0003 0.0003

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 4.6332 6.0533 4.4918 10.022 6.9313 5.6992 7.248 13.4799 12.6542 8.8865
Translational Efficiency 0.8383 0.5616 1.1119 0.6085 0.5359 0.7859 0.6627 0.3505 0.4385 0.4941

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1645 730 541 738 1167 1712 962 413 2812 2442 1503 1151

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 631.19 742.84 1052.50 882.87 625.08 725.35 775.74 891.97 628.65 730.58 875.36 886.14
Standard Deviation 80.23 91.81 121.81 156.59 72.73 85.49 122.95 103.87 77.26 87.79 180.73 140.05
Intensity Change Log 2 — 0.234977 0.737674 0.484127 — 0.214636 0.311532 0.512954 — 0.224892 0.541309 0.498542

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000034 0.000217 0.001189 0.000924 0.000078 0.000243 0.001133 0.001108 0.000052 0.000235 0.001153 0.000990
Bud Neck 0.001569 0.004064 0.006733 0.015416 0.002046 0.008900 0.014479 0.014473 0.001767 0.007454 0.011691 0.015077
Bud Site 0.000746 0.001524 0.004197 0.012458 0.000472 0.004309 0.013925 0.010538 0.000632 0.003476 0.010423 0.011769
Cell Periphery 0.000102 0.000104 0.000138 0.000161 0.000102 0.000127 0.000173 0.000123 0.000102 0.000120 0.000161 0.000148
Cytoplasm 0.200512* 0.102372* 0.030250 0.115323* 0.160113* 0.120681* 0.030016 0.056837 0.183746* 0.115207* 0.030100 0.094337
Cytoplasmic Foci 0.015049 0.011442 0.001719 0.003155 0.013075 0.026071 0.001332 0.004517 0.014230 0.021698 0.001471 0.003644
Eisosomes 0.000021 0.000026 0.000049 0.000034 0.000020 0.000040 0.000026 0.000037 0.000020 0.000036 0.000035 0.000035
Endoplasmic Reticulum 0.000419 0.002713 0.011718 0.006265 0.001034 0.002525 0.006947 0.008624 0.000675 0.002581 0.008665 0.007112
Endosome 0.000744 0.001821 0.001466 0.003165 0.000491 0.003016 0.001670 0.007096 0.000639 0.002659 0.001597 0.004575
Golgi 0.000767 0.000296 0.000102 0.001069 0.000105 0.001411 0.000815 0.002080 0.000492 0.001077 0.000559 0.001432
Lipid Particles 0.001012 0.001882 0.001109 0.000845 0.001659 0.003562 0.000731 0.000885 0.001281 0.003060 0.000867 0.000859
Mitochondria 0.001209 0.005086 0.001558 0.003550 0.001979 0.007928 0.002812 0.003100 0.001528 0.007079 0.002361 0.003389
Mitotic Spindle 0.000118 0.001168 0.002421 0.048820 0.001475 0.011244 0.005946 0.033594 0.000681 0.008232 0.004677 0.043357
None 0.021665 0.007541 0.002465 0.012790 0.015165 0.004861 0.010262 0.006866 0.018967 0.005662 0.007455 0.010664
Nuclear Periphery 0.000348 0.001396 0.001521 0.002201 0.000844 0.002249 0.000879 0.003026 0.000554 0.001994 0.001110 0.002497
Nuclear Periphery Foci 0.000212 0.000530 0.000868 0.001036 0.000319 0.000929 0.001011 0.001018 0.000256 0.000809 0.000960 0.001029
Nucleolus 0.001884 0.003237 0.000940 0.001918 0.002581 0.004603 0.002274 0.000864 0.002174 0.004195 0.001794 0.001540
Nucleus 0.749885* 0.847308* 0.905234* 0.742414* 0.795255* 0.777521* 0.895508* 0.817390* 0.768714* 0.798383* 0.899009* 0.769317*
Peroxisomes 0.001203 0.001724 0.000230 0.000498 0.000635 0.004603 0.001332 0.001857 0.000967 0.003743 0.000935 0.000985
Vacuole 0.002282 0.005085 0.025903 0.023418 0.002405 0.013523 0.008321 0.024262 0.002333 0.011001 0.014650 0.023721
Vacuole Periphery 0.000219 0.000464 0.000190 0.004541 0.000145 0.001654 0.000407 0.001704 0.000188 0.001298 0.000329 0.003523

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -4.47 -16.57 -3.89 -3.36 -0.54 -4.76 -4.11 -10.10 -8.46 -0.34 -6.86 -6.81 -5.53 -4.78 -0.71
Bud Neck -3.20 -8.39 -9.50 -7.43 -5.37 -7.51 -6.82 -7.30 -4.09 -0.84 -8.41 -8.41 -11.98 -7.05 -2.39
Bud Site -1.96 -3.87 -6.81 -6.12 -4.18 -5.15 -5.23 -5.37 -2.19 1.89 -5.22 -5.82 -8.51 -5.26 -0.11
Cell Periphery -0.28 -3.50 -4.51 -3.62 -1.20 -2.28 -3.84 -1.96 0.06 2.21 -2.15 -4.87 -4.97 -2.79 0.77
Cytoplasm 11.76 27.40 16.66 4.07 -8.07 5.14 19.01 15.10 12.93 -1.97 12.35 32.84 21.66 11.16 -7.67
Cytoplasmic Foci 1.47 10.62 9.42 5.16 -1.38 -5.42 7.59 6.78 12.49 -0.68 -4.57 13.04 11.50 13.57 -1.63
Eisosomes -2.69 -10.72 -13.11 -8.37 -0.41 -7.59 -4.00 -9.61 -4.71 -7.33 -8.31 -10.17 -16.23 -8.27 -7.33
Endoplasmic Reticulum -9.24 -19.10 -17.85 -12.48 2.86 -7.67 -13.80 -15.34 -13.20 -6.59 -12.56 -22.79 -23.37 -18.38 -3.67
Endosome -1.78 -3.10 -3.16 -0.40 -1.08 -7.35 -2.68 -2.13 0.58 -0.96 -6.39 -3.27 -3.70 0.98 -1.35
Golgi 1.01 1.48 0.71 -0.47 -1.41 -3.75 -1.65 -1.41 1.92 0.28 -1.70 0.06 0.09 2.07 0.03
Lipid Particles -2.68 -0.93 -1.19 1.64 -0.56 -4.19 2.14 2.16 6.65 -0.75 -5.86 1.45 0.34 6.05 -1.15
Mitochondria -2.19 -0.36 -2.49 1.11 -3.06 -4.80 -0.91 -0.54 5.06 0.64 -5.57 -1.33 -2.42 4.39 -1.19
Mitotic Spindle -2.56 -2.56 -6.85 -6.59 -6.34 -4.66 -3.11 -3.59 -1.62 -2.23 -5.40 -3.99 -7.67 -5.19 -5.89
None 6.73 11.02 6.70 -0.59 -6.79 5.48 2.63 5.29 -0.10 2.26 9.71 7.71 8.29 -1.82 -0.65
Nuclear Periphery -9.28 -16.45 -15.98 -10.23 -8.41 -6.81 -3.98 -6.94 -3.27 -5.88 -9.88 -13.30 -16.04 -7.68 -11.08
Nuclear Periphery Foci -1.85 -4.61 -6.39 -2.97 -2.19 -1.79 -1.87 -2.54 -0.87 0.42 -2.33 -2.43 -6.04 -2.00 0.01
Nucleolus -2.48 5.00 -1.29 1.72 -4.75 -3.75 -0.13 2.92 5.49 2.23 -5.06 0.41 0.56 5.17 0.03
Nucleus -9.06 -14.44 -1.78 5.81 9.92 2.13 -9.31 -2.04 -3.78 5.15 -3.50 -16.95 -2.33 0.50 11.11
Peroxisomes -1.03 1.93 1.43 3.80 -2.12 -5.26 -1.38 0.37 5.09 1.37 -4.69 0.31 1.49 6.00 1.34
Vacuole -5.28 -9.52 -12.03 -11.16 -2.22 -8.15 -6.38 -9.53 -6.99 -7.74 -9.03 -11.17 -15.30 -11.97 -8.49
Vacuole Periphery -1.31 0.17 -1.34 -1.04 -1.38 -3.66 -3.20 -1.37 2.27 -0.58 -3.70 -1.40 -1.58 0.39 -1.37
Standard name
Human Ortholog
Description Single-stranded DNA endonuclease; cleaves single-stranded DNA during nucleotide excision repair to excise damaged DNA; subunit of Nucleotide Excision Repair Factor 3 (NEF3); homolog of human XPG protein
Localization
Cell Percentages nucleus (61%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-3

Rad2

Rad2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Rad2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available