Standard name | |
---|---|
Human Ortholog | |
Description | Single-stranded DNA endonuclease; cleaves single-stranded DNA during nucleotide excision repair to excise damaged DNA; subunit of Nucleotide Excision Repair Factor 3 (NEF3); homolog of human XPG protein |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 2 |
Bud | 0 | 0 | 0 | 2 | 1 | 2 | 2 | 7 | 6 | 4 | 5 | 4 | 0 | 0 | 6 | 0 | 1 | 1 | 2 | 2 | 1 | 1 | 12 | 12 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 2 | 1 | 4 |
Bud Site | 0 | 0 | 0 | 1 | 2 | 3 | 6 | 13 | 14 | 14 | 5 | 9 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 4 | 5 | 7 | 26 | 30 | 36 | 65 | 67 | 34 | 33 | 12 | 39 | 45 | 133 | 170 | 45 | 37 | 93 | 2 | 1 | 4 | 9 | 17 | 21 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 5 | 2 | 18 | 5 | 43 | 0 | 0 | 0 | 1 | 1 | 2 |
Endosome | 0 | 0 | 0 | 0 | 2 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 4 | 8 | 10 | 4 | 19 | 2 | 1 | 0 | 0 | 2 | 16 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 2 | 2 | 0 | 3 | 0 | 0 | 0 | 2 | 2 |
Mitochondria | 38 | 16 | 55 | 164 | 108 | 213 | 334 | 392 | 303 | 403 | 196 | 421 | 12 | 7 | 56 | 20 | 7 | 41 | 3 | 1 | 0 | 0 | 4 | 17 |
Nucleus | 179 | 28 | 67 | 188 | 277 | 202 | 335 | 349 | 190 | 264 | 119 | 214 | 190 | 296 | 322 | 528 | 274 | 665 | 184 | 52 | 75 | 101 | 295 | 365 |
Nuclear Periphery | 1 | 2 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 2 | 1 | 0 | 3 | 0 | 0 | 0 | 0 | 0 | 2 |
Nucleolus | 1 | 0 | 0 | 0 | 1 | 2 | 0 | 2 | 0 | 3 | 4 | 1 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 4 | 16 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 |
SpindlePole | 0 | 0 | 0 | 3 | 0 | 1 | 3 | 8 | 2 | 1 | 0 | 1 | 1 | 0 | 0 | 2 | 0 | 5 | 2 | 3 | 0 | 2 | 9 | 26 |
Vac/Vac Membrane | 26 | 8 | 2 | 4 | 23 | 29 | 24 | 25 | 6 | 21 | 6 | 33 | 53 | 109 | 113 | 66 | 48 | 129 | 23 | 4 | 38 | 18 | 80 | 168 |
Unique Cell Count | 214 | 41 | 96 | 270 | 371 | 362 | 564 | 631 | 375 | 525 | 247 | 499 | 283 | 517 | 623 | 636 | 353 | 871 | 226 | 70 | 124 | 142 | 441 | 673 |
Labelled Cell Count | 249 | 59 | 131 | 388 | 445 | 488 | 771 | 865 | 555 | 745 | 348 | 722 | 302 | 556 | 682 | 693 | 378 | 999 | 226 | 70 | 124 | 142 | 441 | 673 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 4.9 | 4.1 | 3.6 | 3.7 | 3.9 | 3.6 | 3.4 | 3.4 | 3.1 | 3.1 | 3.2 | 2.9 | 5.6 | 5.4 | 4.8 | 6.6 | 7.1 | 7.3 | 5.1 | 5.3 | 5.4 |
Std Deviation (1e-4) | 0.7 | 0.8 | 0.5 | 1.0 | 1.0 | 0.9 | 0.8 | 0.8 | 1.1 | 0.8 | 1.1 | 0.8 | 1.2 | 1.2 | 1.0 | 1.3 | 1.3 | 1.3 | 1.1 | 1.4 | 1.1 |
Intensity Change (Log2) | – | – | – | 0.04 | 0.13 | -0.02 | -0.09 | -0.1 | -0.21 | -0.22 | -0.16 | -0.33 | 0.62 | 0.58 | 0.42 | 0.88 | 0.98 | 1.03 | 0.51 | 0.55 | 0.57 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Endosome | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Golgi | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | 2.6 | 3.2 | 3.5 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 1.2529 | 0.8847 | 1.0645 | 0.4755 | 0.5816 | 0.7649 | 1.4862 | 1.2914 | 1.2409 | 0.9597 | 0.615 | 0.9195 | -0.3104 | 0.1377 | -0.1853 | 0.1571 | -0.2703 | -0.3091 |
Actin | 0.0943 | 0.0002 | 0.0205 | 0.0097 | 0.0044 | 0.0007 | 0.0471 | 0.0001 | 0.019 | 0.0933 | 0.0019 | 0.0051 | 0.0226 | 0.0002 | 0.0094 | 0.0124 | 0.0002 | 0.0008 |
Bud | 0.0008 | 0.0003 | 0.0006 | 0.0004 | 0.0002 | 0.0005 | 0.0012 | 0.0001 | 0.0004 | 0.0019 | 0.0018 | 0.0002 | 0.0007 | 0.0001 | 0.0003 | 0.0001 | 0 | 0.0001 |
Bud Neck | 0.0055 | 0.0007 | 0.0028 | 0.0052 | 0.001 | 0.0055 | 0.0097 | 0.0005 | 0.0061 | 0.0072 | 0.0007 | 0.0017 | 0.0019 | 0.0005 | 0.002 | 0.0006 | 0.0004 | 0.0028 |
Bud Periphery | 0.0019 | 0.0001 | 0.002 | 0.0005 | 0.0005 | 0.0013 | 0.0016 | 0 | 0.0007 | 0.0044 | 0.0023 | 0.0004 | 0.001 | 0.0001 | 0.0006 | 0.0001 | 0 | 0.0001 |
Bud Site | 0.0081 | 0.0021 | 0.0085 | 0.001 | 0.0002 | 0.0034 | 0.0236 | 0.0007 | 0.0022 | 0.0138 | 0.0097 | 0.0004 | 0.0025 | 0.0008 | 0.0036 | 0.0003 | 0.0001 | 0.0002 |
Cell Periphery | 0.0006 | 0.0001 | 0.0005 | 0.0001 | 0.0001 | 0.0002 | 0.0006 | 0 | 0.0001 | 0.0023 | 0.0001 | 0 | 0.0002 | 0.0001 | 0.0004 | 0 | 0 | 0 |
Cytoplasm | 0.0382 | 0.1381 | 0.0451 | 0.0914 | 0.0717 | 0.0535 | 0.0566 | 0.0987 | 0.0456 | 0.0294 | 0.0164 | 0.0383 | 0.0551 | 0.0715 | 0.0561 | 0.0646 | 0.0434 | 0.0489 |
Cytoplasmic Foci | 0.0141 | 0.0035 | 0.0116 | 0.0137 | 0.0027 | 0.0021 | 0.0279 | 0.0012 | 0.0042 | 0.0062 | 0.0068 | 0.0028 | 0.0097 | 0.0028 | 0.0029 | 0.0077 | 0.001 | 0.0023 |
Eisosomes | 0.0008 | 0 | 0.0007 | 0.0001 | 0.0001 | 0 | 0.0003 | 0 | 0.0001 | 0.0006 | 0 | 0 | 0.0004 | 0 | 0.0004 | 0.0001 | 0 | 0 |
Endoplasmic Reticulum | 0.008 | 0.0076 | 0.0031 | 0.0034 | 0.003 | 0.0024 | 0.0056 | 0.0037 | 0.0023 | 0.0023 | 0.0003 | 0.0014 | 0.0029 | 0.0029 | 0.0027 | 0.0023 | 0.0013 | 0.0012 |
Endosome | 0.0251 | 0.0027 | 0.0079 | 0.0087 | 0.0145 | 0.0034 | 0.0413 | 0.0013 | 0.0063 | 0.0168 | 0.004 | 0.0037 | 0.0057 | 0.0031 | 0.003 | 0.0104 | 0.001 | 0.0021 |
Golgi | 0.0086 | 0.0001 | 0.0031 | 0.0036 | 0.0045 | 0.0006 | 0.0105 | 0 | 0.0084 | 0.0143 | 0.0037 | 0.0023 | 0.0029 | 0.0001 | 0.0006 | 0.0031 | 0 | 0.0006 |
Lipid Particles | 0.0212 | 0.0004 | 0.0085 | 0.0037 | 0.0241 | 0.0083 | 0.0253 | 0.0001 | 0.0095 | 0.0047 | 0.0037 | 0.0035 | 0.0064 | 0.0005 | 0.0017 | 0.0139 | 0.0001 | 0.0041 |
Mitochondria | 0.0093 | 0.0004 | 0.0171 | 0.0039 | 0.008 | 0.0008 | 0.0063 | 0.0002 | 0.0026 | 0.0172 | 0.0109 | 0.009 | 0.0042 | 0.0005 | 0.0066 | 0.0112 | 0.0004 | 0.0092 |
None | 0.0373 | 0.0675 | 0.0299 | 0.0537 | 0.0456 | 0.0263 | 0.0371 | 0.0502 | 0.043 | 0.0352 | 0.0044 | 0.0203 | 0.1275 | 0.1559 | 0.1227 | 0.1589 | 0.2213 | 0.0931 |
Nuclear Periphery | 0.0545 | 0.0271 | 0.0285 | 0.0195 | 0.0451 | 0.0199 | 0.0274 | 0.0149 | 0.0123 | 0.0097 | 0.005 | 0.01 | 0.0179 | 0.0209 | 0.0181 | 0.0178 | 0.0275 | 0.0095 |
Nucleolus | 0.0056 | 0.0066 | 0.007 | 0.0035 | 0.0043 | 0.0112 | 0.0044 | 0.0027 | 0.0042 | 0.0048 | 0.0032 | 0.0029 | 0.0036 | 0.0045 | 0.0059 | 0.003 | 0.0029 | 0.0074 |
Nucleus | 0.6259 | 0.7243 | 0.7727 | 0.7185 | 0.7524 | 0.8133 | 0.6073 | 0.8088 | 0.8101 | 0.7176 | 0.9098 | 0.878 | 0.6891 | 0.7145 | 0.7398 | 0.6381 | 0.6928 | 0.7788 |
Peroxisomes | 0.0126 | 0.0001 | 0.0072 | 0.0059 | 0.001 | 0.0021 | 0.0092 | 0 | 0.002 | 0.0076 | 0.0079 | 0.0055 | 0.0096 | 0.0001 | 0.0021 | 0.0295 | 0 | 0.0015 |
Punctate Nuclear | 0.0153 | 0.0152 | 0.0174 | 0.051 | 0.0111 | 0.041 | 0.0488 | 0.015 | 0.0194 | 0.0038 | 0.0057 | 0.0133 | 0.0341 | 0.019 | 0.0187 | 0.0248 | 0.0064 | 0.0361 |
Vacuole | 0.0085 | 0.0025 | 0.004 | 0.002 | 0.0022 | 0.0032 | 0.0056 | 0.0013 | 0.001 | 0.0052 | 0.0014 | 0.0008 | 0.0015 | 0.0018 | 0.002 | 0.0006 | 0.0009 | 0.0008 |
Vacuole Periphery | 0.0038 | 0.0004 | 0.0013 | 0.0006 | 0.0031 | 0.0004 | 0.0025 | 0.0002 | 0.0005 | 0.0014 | 0.0003 | 0.0003 | 0.0004 | 0.0003 | 0.0004 | 0.0004 | 0.0003 | 0.0003 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 4.6332 | 6.0533 | 4.4918 | 10.022 | 6.9313 | 5.6992 | 7.248 | 13.4799 | 12.6542 | 8.8865 |
Translational Efficiency | 0.8383 | 0.5616 | 1.1119 | 0.6085 | 0.5359 | 0.7859 | 0.6627 | 0.3505 | 0.4385 | 0.4941 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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