Standard name | |
---|---|
Human Ortholog | |
Description | Beta3-like subunit of the yeast AP-3 complex; functions in transport of alkaline phosphatase to the vacuole via the alternate pathway; exists in both cytosolic and peripherally associated membrane-bound pools |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 42 | 40 | 30 | 6 | 6 | 5 | 13 | 27 | 16 | 19 | 20 | 12 | 14 | 14 | 12 | 19 | 19 | 32 | 20 | 20 | 45 | 10 | 18 | 26 |
Bud | 3 | 5 | 9 | 1 | 6 | 20 | 26 | 23 | 22 | 24 | 34 | 15 | 1 | 1 | 1 | 14 | 3 | 5 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 4 | 4 | 5 | 3 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 2 | 1 | 2 | 0 | 0 | 1 | 0 | 3 | 1 | 5 | 2 | 2 | 0 | 0 | 0 | 3 | 1 | 2 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 4 | 10 | 10 | 6 | 20 | 12 | 16 | 27 | 11 | 10 | 6 | 5 | 7 | 7 | 12 | 72 | 26 | 31 | 0 | 0 | 0 | 1 | 0 | 0 |
Endoplasmic Reticulum | 19 | 15 | 9 | 2 | 6 | 17 | 12 | 32 | 17 | 14 | 7 | 15 | 17 | 17 | 14 | 22 | 11 | 8 | 1 | 2 | 1 | 2 | 3 | 3 |
Endosome | 50 | 17 | 92 | 39 | 60 | 26 | 85 | 100 | 52 | 62 | 37 | 52 | 19 | 18 | 18 | 162 | 103 | 80 | 65 | 41 | 87 | 28 | 35 | 39 |
Golgi | 237 | 163 | 201 | 47 | 99 | 28 | 21 | 35 | 11 | 24 | 16 | 28 | 47 | 54 | 70 | 126 | 89 | 149 | 299 | 195 | 309 | 142 | 197 | 244 |
Mitochondria | 151 | 158 | 175 | 175 | 205 | 364 | 363 | 469 | 397 | 441 | 340 | 262 | 12 | 12 | 5 | 35 | 26 | 37 | 3 | 7 | 6 | 5 | 8 | 5 |
Nucleus | 1 | 0 | 0 | 1 | 1 | 1 | 0 | 3 | 1 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 1 | 2 | 3 | 3 | 6 | 3 | 8 | 6 | 5 | 4 | 6 | 0 | 0 | 0 | 0 | 1 | 4 | 1 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 4 | 2 | 3 | 8 | 7 | 8 | 13 | 23 | 9 | 15 | 8 | 10 | 1 | 1 | 1 | 3 | 1 | 2 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 2 | 30 | 14 | 28 | 8 | 36 | 32 | 45 | 36 | 26 | 31 | 1 | 0 | 0 | 9 | 9 | 5 | 10 | 4 | 32 | 12 | 17 | 19 |
SpindlePole | 2 | 2 | 12 | 2 | 3 | 2 | 6 | 13 | 12 | 9 | 6 | 10 | 4 | 4 | 2 | 41 | 33 | 25 | 1 | 0 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 22 | 16 | 17 | 1 | 13 | 8 | 6 | 9 | 6 | 12 | 4 | 6 | 13 | 14 | 19 | 21 | 17 | 15 | 3 | 4 | 0 | 0 | 0 | 2 |
Unique Cell Count | 347 | 255 | 409 | 238 | 345 | 430 | 483 | 638 | 490 | 555 | 412 | 350 | 101 | 101 | 126 | 440 | 261 | 307 | 411 | 278 | 488 | 205 | 284 | 346 |
Labelled Cell Count | 537 | 432 | 592 | 305 | 457 | 506 | 600 | 808 | 610 | 681 | 514 | 457 | 136 | 142 | 154 | 527 | 340 | 395 | 411 | 278 | 488 | 205 | 284 | 346 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 11.1 | 11.7 | 9.9 | 9.0 | 8.9 | 7.6 | 7.6 | 7.6 | 7.3 | 7.3 | 7.1 | 7.5 | 10.5 | 10.5 | 11.0 | 7.8 | 8.6 | 10.0 | 11.2 | 11.9 | 12.6 |
Std Deviation (1e-4) | 1.5 | 1.6 | 1.4 | 1.6 | 1.8 | 1.4 | 1.7 | 1.7 | 1.4 | 1.7 | 1.6 | 1.7 | 1.4 | 1.4 | 1.4 | 1.2 | 1.5 | 1.7 | 1.9 | 2.1 | 2.2 |
Intensity Change (Log2) | – | – | – | -0.14 | -0.16 | -0.4 | -0.4 | -0.39 | -0.45 | -0.44 | -0.48 | -0.4 | 0.08 | 0.08 | 0.14 | -0.35 | -0.21 | 0.01 | 0.17 | 0.26 | 0.34 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | -2.6 | -3.6 | -4.5 | -3.2 | -2.2 | -2.8 | -2.7 | -1.5 | -2.3 | 2.1 | 2.1 | 0.8 | -1.9 | 0 | 1.5 |
Bud | 0 | -0.5 | 1.9 | 2.4 | 1.3 | 1.9 | 1.8 | 3.9 | 1.6 | 0 | 0 | 0 | 0.9 | 0 | -0.5 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 0.1 | 2.3 | 0.3 | 0.8 | 1.5 | -0.2 | -0.7 | -1.0 | -1.0 | 0 | 0 | 3.5 | 6.9 | 4.2 | 4.4 |
Endoplasmic Reticulum | 0 | -0.5 | 1.5 | 0.3 | 2.3 | 1.1 | 0.3 | -0.5 | 1.6 | 6.0 | 6.0 | 4.3 | 2.2 | 1.5 | 0.4 |
Endosome | -1.9 | -1.7 | -6.8 | -1.8 | -2.8 | -4.8 | -4.7 | -5.3 | -2.7 | -0.8 | -1.0 | -2.0 | 4.6 | 4.7 | 1.1 |
Golgi | -7.4 | -5.7 | -13.9 | -15.4 | -16.5 | -16.5 | -16.3 | -14.7 | -12.3 | -0.5 | 0.8 | 1.3 | -6.1 | -3.8 | -0.2 |
Mitochondria | 7.6 | 4.6 | 12.6 | 9.8 | 10.0 | 11.9 | 11.7 | 11.8 | 8.9 | -5.8 | -5.8 | -8.1 | -11.8 | -9.0 | -8.9 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 1.4 | 2.2 | 2.9 | 1.4 | 2.2 | 1.5 | 2.2 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | -0.7 | 0.4 | -3.8 | 0.1 | -1.6 | 1.0 | -0.5 | -0.6 | 0.8 | -2.4 | -2.8 | -3.1 | -3.7 | -2.1 | -3.5 |
SpindlePole | -1.8 | -2.0 | -2.8 | -1.8 | -0.9 | -0.4 | -1.4 | -1.4 | -0.1 | 0 | 0 | 0 | 3.8 | 4.9 | 3.1 |
Vacuole | -2.8 | -0.3 | -2.0 | -2.7 | -2.8 | -2.8 | -1.8 | -2.9 | -2.0 | 3.3 | 3.7 | 4.3 | 0.4 | 1.4 | 0.5 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 5.2919 | 6.7047 | 6.4852 | 6.227 | 5.2571 | 6.3086 | 7.5376 | 8.8978 | 8.184 | 7.5625 | 7.9911 | 8.1265 | 9.0973 | 9.8172 | 9.239 | 8.9904 | 8.6611 | 9.31 |
Actin | 0.0294 | 0.0076 | 0.0244 | 0.0131 | 0.0479 | 0.0116 | 0.0463 | 0.0185 | 0.0287 | 0.016 | 0.0041 | 0.0182 | 0.0106 | 0.0293 | 0.0086 | 0.0028 | 0.0021 | 0.0253 |
Bud | 0.0004 | 0.0006 | 0.0004 | 0.0002 | 0.0004 | 0.0001 | 0.0004 | 0.0006 | 0.0004 | 0 | 0.0001 | 0.0001 | 0.0002 | 0.0003 | 0.0001 | 0.0001 | 0 | 0.0001 |
Bud Neck | 0.0008 | 0.0005 | 0.0005 | 0.0002 | 0.0015 | 0.0006 | 0.0076 | 0.0006 | 0.0004 | 0.0002 | 0.0001 | 0.0003 | 0.0003 | 0.0002 | 0.0002 | 0.0001 | 0.0002 | 0.0003 |
Bud Periphery | 0.0003 | 0.0002 | 0.0002 | 0.0001 | 0.0003 | 0.0001 | 0.0005 | 0.0002 | 0.0005 | 0 | 0 | 0.0001 | 0.0001 | 0.0001 | 0 | 0 | 0 | 0.0001 |
Bud Site | 0.0053 | 0.0099 | 0.0009 | 0.0003 | 0.0009 | 0.0002 | 0.0054 | 0.0142 | 0.0025 | 0.0001 | 0.0001 | 0.0002 | 0.0022 | 0.0008 | 0.0002 | 0.0001 | 0.0001 | 0.0001 |
Cell Periphery | 0.0001 | 0.0001 | 0.0001 | 0 | 0.0001 | 0 | 0.0003 | 0 | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 0.0045 | 0.0317 | 0.0044 | 0.0041 | 0.0037 | 0.0029 | 0.0078 | 0.0081 | 0.0026 | 0.0017 | 0.0017 | 0.0035 | 0.0041 | 0.005 | 0.0033 | 0.0046 | 0.0042 | 0.0025 |
Cytoplasmic Foci | 0.128 | 0.1475 | 0.1268 | 0.0941 | 0.1321 | 0.1012 | 0.1155 | 0.1664 | 0.1411 | 0.1247 | 0.147 | 0.0901 | 0.1644 | 0.1541 | 0.1464 | 0.1249 | 0.1439 | 0.1183 |
Eisosomes | 0.0001 | 0 | 0.0001 | 0 | 0.0003 | 0 | 0.0007 | 0 | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0.0023 | 0.0014 | 0.0007 | 0.0009 | 0.001 | 0.0008 | 0.0024 | 0.0004 | 0.0008 | 0.0002 | 0.0002 | 0.0004 | 0.0007 | 0.0019 | 0.0003 | 0.0003 | 0.0004 | 0.0007 |
Endosome | 0.2773 | 0.3084 | 0.2761 | 0.4432 | 0.3638 | 0.2351 | 0.2576 | 0.2292 | 0.2286 | 0.3085 | 0.2304 | 0.1571 | 0.2218 | 0.2427 | 0.2166 | 0.2836 | 0.2286 | 0.1791 |
Golgi | 0.4659 | 0.4632 | 0.5283 | 0.4151 | 0.4046 | 0.615 | 0.4633 | 0.5234 | 0.5476 | 0.5325 | 0.5929 | 0.7086 | 0.5779 | 0.5434 | 0.6046 | 0.5726 | 0.5986 | 0.6449 |
Lipid Particles | 0.0381 | 0.0051 | 0.0058 | 0.0034 | 0.0115 | 0.0078 | 0.0072 | 0.0024 | 0.0036 | 0.002 | 0.0041 | 0.0025 | 0.0038 | 0.0018 | 0.0022 | 0.002 | 0.0028 | 0.0025 |
Mitochondria | 0.0078 | 0.0035 | 0.0074 | 0.0118 | 0.0043 | 0.0122 | 0.0041 | 0.0023 | 0.0034 | 0.0015 | 0.0012 | 0.0094 | 0.0018 | 0.0016 | 0.0026 | 0.0021 | 0.0014 | 0.0037 |
None | 0.0019 | 0.0002 | 0.003 | 0 | 0.0007 | 0.0001 | 0.0082 | 0.0001 | 0.0145 | 0 | 0 | 0 | 0.0001 | 0.0002 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0.0045 | 0.0003 | 0.0011 | 0.0002 | 0.0011 | 0.0002 | 0.0269 | 0.0001 | 0.0006 | 0.0001 | 0 | 0.0001 | 0.0003 | 0.0002 | 0 | 0.0001 | 0.0001 | 0.0001 |
Nucleolus | 0.0003 | 0.0001 | 0.0005 | 0 | 0.0001 | 0 | 0.0017 | 0 | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0.0007 | 0.0002 | 0.0014 | 0 | 0.0002 | 0.0001 | 0.0139 | 0 | 0.0003 | 0 | 0 | 0 | 0.0001 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0.0189 | 0.0138 | 0.0129 | 0.0083 | 0.0198 | 0.0085 | 0.0104 | 0.0321 | 0.0223 | 0.0076 | 0.017 | 0.0082 | 0.0104 | 0.0146 | 0.0135 | 0.0041 | 0.0162 | 0.0206 |
Punctate Nuclear | 0.0069 | 0.0003 | 0.002 | 0.0001 | 0.0016 | 0.0001 | 0.0172 | 0.0002 | 0.0004 | 0.0001 | 0.0001 | 0.0001 | 0.0002 | 0.0026 | 0.0001 | 0.0001 | 0.0001 | 0.0001 |
Vacuole | 0.004 | 0.0037 | 0.0015 | 0.0017 | 0.0022 | 0.0013 | 0.0011 | 0.0006 | 0.0007 | 0.0033 | 0.0003 | 0.0004 | 0.0005 | 0.0005 | 0.0004 | 0.001 | 0.0005 | 0.0005 |
Vacuole Periphery | 0.0025 | 0.0016 | 0.0016 | 0.0031 | 0.0021 | 0.0018 | 0.0016 | 0.0006 | 0.0007 | 0.0016 | 0.0007 | 0.0007 | 0.0006 | 0.0007 | 0.0007 | 0.0015 | 0.0009 | 0.001 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 69.4736 | 45.4626 | 40.9642 | 51.5645 | 47.0983 | 58.0895 | 46.5828 | 44.7366 | 50.3915 | 55.5724 |
Translational Efficiency | 0.5956 | 0.6363 | 0.6928 | 0.5305 | 0.5699 | 0.618 | 0.6264 | 0.5942 | 0.5881 | 0.5771 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✔ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
654 | 1284 | 237 | 1459 | 2562 | 2004 | 144 | 1518 | 3216 | 3288 | 381 | 2977 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 1061.20 | 1053.67 | 1292.56 | 1252.85 | 1001.94 | 1049.64 | 1260.77 | 1050.28 | 1013.99 | 1051.21 | 1280.54 | 1149.56 |
Standard Deviation | 137.79 | 167.53 | 220.32 | 203.20 | 136.62 | 147.58 | 224.64 | 153.63 | 138.92 | 155.69 | 222.50 | 206.22 |
Intensity Change Log 2 | — | -0.010273 | 0.284535 | 0.239517 | — | 0.067098 | 0.331509 | 0.067978 | — | 0.027820 | 0.307538 | 0.158754 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.015325 | 0.009094 | 0.015238 | 0.033444 | 0.007053 | 0.006053 | 0.020779 | 0.001109 | 0.008735 | 0.007241 | 0.017332 | 0.016956 |
Bud Neck | 0.000872 | 0.000202 | 0.000855 | 0.000676 | 0.000073 | 0.000113 | 0.001049 | 0.002930 | 0.000235 | 0.000148 | 0.000928 | 0.001825 |
Bud Site | 0.018395 | 0.002961 | 0.002823 | 0.018121 | 0.000539 | 0.001054 | 0.006079 | 0.011634 | 0.004170 | 0.001799 | 0.004053 | 0.014813 |
Cell Periphery | 0.000126 | 0.000063 | 0.000127 | 0.000239 | 0.000046 | 0.000089 | 0.000108 | 0.000082 | 0.000062 | 0.000079 | 0.000119 | 0.000159 |
Cytoplasm | 0.009506 | 0.002581 | 0.000448 | 0.011381 | 0.000510 | 0.001096 | 0.000780 | 0.013670 | 0.002340 | 0.001676 | 0.000574 | 0.012548 |
Cytoplasmic Foci | 0.222820* | 0.259556* | 0.172954 | 0.150997 | 0.167419 | 0.210200* | 0.234625* | 0.230181* | 0.178685 | 0.229474* | 0.196263* | 0.191374* |
Eisosomes | 0.000059 | 0.000050 | 0.000073 | 0.000114 | 0.000069 | 0.000045 | 0.000065 | 0.000028 | 0.000067 | 0.000047 | 0.000070 | 0.000070 |
Endoplasmic Reticulum | 0.000441 | 0.000513 | 0.002229 | 0.001403 | 0.000349 | 0.000187 | 0.001084 | 0.000468 | 0.000367 | 0.000314 | 0.001796 | 0.000927 |
Endosome | 0.331138* | 0.166982 | 0.174756 | 0.195292 | 0.267639* | 0.207906* | 0.256148* | 0.428649* | 0.280552* | 0.191924 | 0.205518* | 0.314283* |
Golgi | 0.331583* | 0.523976* | 0.561478* | 0.529749* | 0.520466* | 0.547186* | 0.392929* | 0.138667* | 0.482055* | 0.538122* | 0.497774* | 0.330333* |
Lipid Particles | 0.004256 | 0.012575 | 0.031610 | 0.008476 | 0.011922 | 0.003548 | 0.029790 | 0.021965 | 0.010363 | 0.007073 | 0.030922 | 0.015354 |
Mitochondria | 0.004433 | 0.004530 | 0.000931 | 0.003877 | 0.011367 | 0.006909 | 0.000471 | 0.007124 | 0.009957 | 0.005980 | 0.000757 | 0.005533 |
Mitotic Spindle | 0.008686 | 0.002669 | 0.002439 | 0.008409 | 0.000697 | 0.000997 | 0.004431 | 0.049689 | 0.002322 | 0.001650 | 0.003191 | 0.029458 |
None | 0.000014 | 0.000048 | 0.000012 | 0.000028 | 0.000011 | 0.000094 | 0.000010 | 0.000221 | 0.000012 | 0.000076 | 0.000011 | 0.000126 |
Nuclear Periphery | 0.000064 | 0.000031 | 0.000212 | 0.000066 | 0.000021 | 0.000048 | 0.000207 | 0.000522 | 0.000030 | 0.000041 | 0.000210 | 0.000298 |
Nuclear Periphery Foci | 0.000323 | 0.000231 | 0.001753 | 0.000434 | 0.000104 | 0.000322 | 0.006011 | 0.005778 | 0.000148 | 0.000287 | 0.003362 | 0.003159 |
Nucleolus | 0.000008 | 0.000060 | 0.000006 | 0.000008 | 0.000018 | 0.000082 | 0.000013 | 0.001514 | 0.000016 | 0.000073 | 0.000009 | 0.000776 |
Nucleus | 0.000025 | 0.000179 | 0.000016 | 0.000028 | 0.000015 | 0.000329 | 0.000043 | 0.001256 | 0.000017 | 0.000271 | 0.000026 | 0.000654 |
Peroxisomes | 0.013808 | 0.005178 | 0.004281 | 0.006939 | 0.002485 | 0.006094 | 0.008530 | 0.016959 | 0.004788 | 0.005736 | 0.005886 | 0.012048 |
Vacuole | 0.028304 | 0.004856 | 0.020957 | 0.022819 | 0.004051 | 0.003407 | 0.030959 | 0.056460 | 0.008983 | 0.003973 | 0.024737 | 0.039973 |
Vacuole Periphery | 0.009815 | 0.003664 | 0.006805 | 0.007501 | 0.005146 | 0.004242 | 0.005890 | 0.011095 | 0.006096 | 0.004016 | 0.006459 | 0.009334 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | 2.12 | 0.06 | -4.49 | -7.26 | -3.63 | 0.69 | -1.74 | 6.00 | 4.31 | 2.51 | 1.19 | -2.15 | -4.63 | -5.55 | 0.09 |
Bud Neck | 5.24 | 0.20 | 1.26 | -6.38 | 0.96 | -1.56 | -4.63 | -7.02 | -6.91 | -4.22 | 2.74 | -5.77 | -7.48 | -7.93 | -3.90 |
Bud Site | 6.03 | 6.09 | 0.14 | -9.55 | -9.65 | -2.67 | -3.95 | -9.43 | -8.93 | -3.19 | 4.17 | 0.32 | -9.75 | -13.34 | -9.60 |
Cell Periphery | 3.28 | 0.01 | -4.20 | -8.13 | -4.52 | -0.83 | -4.11 | -1.15 | 0.11 | 0.72 | -0.52 | -4.82 | -4.95 | -2.19 | -1.87 |
Cytoplasm | 3.62 | 5.13 | -1.00 | -6.20 | -9.06 | -1.59 | -1.62 | -8.18 | -7.70 | -8.02 | 1.33 | 4.69 | -9.59 | -10.18 | -11.78 |
Cytoplasmic Foci | -4.91 | 4.43 | 10.27 | 19.12 | 2.21 | -10.11 | -5.03 | -11.71 | -3.38 | 0.39 | -13.86 | -2.17 | -3.20 | 9.61 | 0.64 |
Eisosomes | 1.23 | -1.17 | -5.07 | -7.19 | -3.12 | 4.31 | 0.37 | 8.86 | 3.68 | 3.80 | 4.57 | -0.41 | -0.59 | -4.67 | 0.00 |
Endoplasmic Reticulum | -0.68 | -3.47 | -3.95 | -3.63 | 1.44 | 1.90 | -3.71 | -1.42 | -4.74 | 3.16 | 0.72 | -4.30 | -4.23 | -5.03 | 2.46 |
Endosome | 17.11 | 12.56 | 13.97 | -5.05 | -2.10 | 11.32 | 0.76 | -20.21 | -28.50 | -10.49 | 19.80 | 8.56 | -5.45 | -23.04 | -11.62 |
Golgi | -16.81 | -11.63 | -16.22 | -0.25 | 1.86 | -4.06 | 5.79 | 58.69* | 61.11* | 11.60 | -9.78 | -1.07 | 21.85 | 31.15 | 11.17 |
Lipid Particles | -3.92 | -4.21 | -2.63 | 1.97 | 3.55 | 6.09 | -2.02 | -4.12 | -8.44 | 0.85 | 2.59 | -3.91 | -3.25 | -6.02 | 2.90 |
Mitochondria | -0.10 | 3.51 | 0.49 | 0.63 | -4.31 | 3.02 | 9.51 | 2.81 | -0.12 | -6.40 | 3.47 | 9.71 | 4.03 | 0.54 | -7.50 |
Mitotic Spindle | 3.15 | 3.06 | 0.22 | -4.18 | -3.85 | -0.66 | -2.16 | -13.12 | -13.07 | -11.20 | 1.39 | -0.68 | -13.08 | -13.63 | -11.95 |
None | -1.37 | 0.53 | -3.15 | 0.81 | -4.94 | -1.53 | 0.62 | -3.37 | -1.71 | -3.39 | -1.86 | 0.36 | -3.57 | -1.26 | -3.59 |
Nuclear Periphery | 1.99 | -3.31 | -0.05 | -1.53 | 3.08 | -0.77 | -3.34 | -4.38 | -3.97 | -2.52 | -0.50 | -5.37 | -4.52 | -4.09 | -1.37 |
Nuclear Periphery Foci | 0.90 | -2.35 | -1.39 | -1.94 | 2.14 | -0.85 | -1.73 | -6.56 | -6.12 | -0.10 | -0.89 | -2.38 | -6.76 | -6.17 | -0.08 |
Nucleolus | -1.60 | 0.71 | -0.40 | 1.58 | -1.09 | -0.94 | 0.33 | -3.35 | -3.20 | -3.37 | -1.33 | 0.65 | -3.34 | -3.07 | -3.37 |
Nucleus | -1.19 | 2.16 | -0.87 | 1.15 | -3.81 | -1.23 | -1.16 | -3.77 | -2.41 | -3.69 | -1.55 | -0.75 | -3.79 | -1.87 | -3.75 |
Peroxisomes | 4.20 | 3.93 | 3.49 | -1.50 | -1.53 | -3.85 | -2.53 | -11.73 | -7.35 | -3.27 | -1.21 | -0.79 | -8.80 | -6.64 | -4.16 |
Vacuole | 8.60 | 2.00 | 1.64 | -9.52 | -0.83 | 1.26 | -3.79 | -18.07 | -18.30 | -3.58 | 6.89 | -4.54 | -16.95 | -20.46 | -4.43 |
Vacuole Periphery | 4.60 | 1.70 | 1.63 | -4.68 | -0.51 | 1.40 | -0.36 | -5.44 | -6.07 | -2.20 | 3.77 | -0.32 | -4.41 | -7.54 | -2.24 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
---|
Gene | Images |
---|