ORF | |
---|---|
Human Ortholog | |
Description | Protein of unknown function; predicted to contain a single transmembrane domain; mutant has increased aneuploidy tolerance; localized to both the mitochondrial outer membrane and the plasma membrane; protein abundance increases in response to DNA replication stress |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 53 | 51 | 34 | 36 | 124 | 69 | 72 | 58 | 41 | – | 23 | 52 | 26 | 45 | 49 | 25 | 24 | 18 | 12 | 29 | 9 | 6 | 4 | 9 |
Bud | 20 | 34 | 13 | 6 | 33 | 14 | 26 | 18 | 21 | – | 42 | 23 | 8 | 4 | 12 | 6 | 3 | 2 | 2 | 2 | 0 | 0 | 0 | 3 |
Bud Neck | 0 | 0 | 1 | 1 | 3 | 1 | 4 | 2 | 1 | – | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 11 | 44 | 30 | 4 | 5 | 7 |
Bud Site | 0 | 0 | 2 | 1 | 0 | 0 | 0 | 0 | 0 | – | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 181 | 253 | 230 | 236 | 269 | 249 | 262 | 139 | 132 | – | 74 | 169 | 149 | 205 | 239 | 436 | 276 | 321 | 245 | 288 | 342 | 84 | 222 | 263 |
Cytoplasm | 88 | 247 | 202 | 217 | 207 | 256 | 292 | 230 | 191 | – | 211 | 333 | 87 | 87 | 114 | 76 | 46 | 68 | 16 | 107 | 31 | 4 | 2 | 7 |
Endoplasmic Reticulum | 12 | 19 | 2 | 7 | 12 | 16 | 24 | 9 | 3 | – | 1 | 11 | 9 | 9 | 17 | 2 | 4 | 5 | 9 | 26 | 14 | 0 | 4 | 5 |
Endosome | 0 | 0 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 3 | 1 | 0 | 0 | 2 |
Golgi | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 3 | 2 | 1 | 1 | 1 | 1 | 0 | 0 | 0 |
Mitochondria | 2 | 4 | 2 | 10 | 23 | 69 | 78 | 37 | 105 | – | 127 | 92 | 1 | 1 | 0 | 9 | 2 | 3 | 4 | 9 | 10 | 1 | 3 | 3 |
Nucleus | 0 | 1 | 4 | 2 | 2 | 2 | 3 | 5 | 4 | – | 6 | 4 | 2 | 2 | 2 | 22 | 5 | 19 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 2 | 0 | 0 | 0 | 1 | 0 | 0 | 2 | 0 | – | 7 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | – | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 1 | 2 | 6 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 4 | 2 | 5 | 6 | 9 | 3 | 3 | 4 | 1 | – | 5 | 7 | 0 | 0 | 0 | 1 | 2 | 4 | 0 | 0 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 0 | 0 | 6 | 2 | 6 | 0 | 2 | 4 | 0 | – | 9 | 10 | 0 | 0 | 2 | 8 | 1 | 5 | 1 | 5 | 7 | 0 | 1 | 1 |
Unique Cell Count | 254 | 448 | 357 | 369 | 503 | 466 | 545 | 387 | 351 | 379 | 521 | 201 | 247 | 307 | 511 | 324 | 382 | 311 | 522 | 457 | 106 | 246 | 310 | |
Labelled Cell Count | 363 | 611 | 503 | 525 | 690 | 679 | 766 | 508 | 500 | 508 | 705 | 283 | 355 | 442 | 588 | 365 | 446 | 311 | 522 | 457 | 106 | 246 | 310 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 7.9 | 8.2 | 7.6 | 7.1 | 7.4 | 6.0 | 6.0 | 6.4 | 5.3 | – | 5.0 | 5.8 | 8.3 | 8.3 | 8.2 | 15.2 | 15.2 | 14.6 | 7.4 | 7.2 | 7.7 |
Std Deviation (1e-4) | 1.1 | 1.3 | 0.8 | 0.9 | 1.0 | 0.9 | 0.7 | 0.8 | 0.8 | – | 0.9 | 0.8 | 1.1 | 1.4 | 1.0 | 2.6 | 2.7 | 2.4 | 1.3 | 1.1 | 1.3 |
Intensity Change (Log2) | – | – | – | -0.08 | -0.04 | -0.33 | -0.33 | -0.24 | -0.5 | – | -0.59 | -0.38 | 0.12 | 0.14 | 0.11 | 1.01 | 1.01 | 0.95 | -0.03 | -0.07 | 0.03 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0.1 | 5.6 | 2.3 | 1.7 | 2.3 | 0.9 | – | -1.8 | 0.2 | 1.2 | 3.1 | 2.5 | -2.7 | -1.0 | -2.6 |
Bud | -1.7 | 1.9 | -0.5 | 0.8 | 0.7 | 1.5 | – | 3.8 | 0.6 | 0.2 | -1.5 | 0.2 | -2.4 | -2.3 | -3.0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | -0.1 | -3.2 | -3.2 | -4.8 | -7.8 | -7.1 | – | -12.4 | -9.4 | 2.4 | 5.0 | 3.8 | 7.2 | 6.2 | 6.1 |
Cytoplasm | 0.6 | -4.5 | -0.5 | -0.9 | 0.8 | -0.6 | – | -0.2 | 2.2 | -3.0 | -5.2 | -5.0 | -13.0 | -11.5 | -10.9 |
Endoplasmic Reticulum | 0 | 2.1 | 2.8 | 3.4 | 2.0 | 0 | – | 0 | 1.9 | 0 | 0 | 3.8 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 2.3 | 3.5 | 7.2 | 7.1 | 5.5 | 10.9 | – | 11.8 | 8.0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 2.7 | 0 | 3.0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0.2 | 0.4 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 0 | 0 | 0 | – | 0.7 | 0.3 | 0 | 0 | 0 | -0.1 | 0 | -0.4 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 3.4152 | 3.5633 | 3.3763 | 2.9743 | 2.733 | 3.5304 | 5.0699 | 5.4276 | 5.2286 | 4.4858 | 3.9967 | 5.0332 | 6.223 | 5.4919 | 5.4244 | 5.3459 | 5.1532 | 5.4263 |
Actin | 0.0569 | 0.0134 | 0.0292 | 0.0073 | 0.066 | 0.0178 | 0.0144 | 0.0006 | 0.0046 | 0.0115 | 0.0342 | 0.0036 | 0.0094 | 0.0068 | 0.0028 | 0.0172 | 0.0002 | 0.0025 |
Bud | 0.0011 | 0.0011 | 0.0026 | 0.0023 | 0.0008 | 0.0011 | 0.0007 | 0.0002 | 0.0007 | 0.0005 | 0.0021 | 0.0004 | 0.0003 | 0.0007 | 0.0005 | 0.0006 | 0.0004 | 0.0004 |
Bud Neck | 0.0141 | 0.0044 | 0.0217 | 0.0141 | 0.0373 | 0.0426 | 0.0032 | 0.0021 | 0.0041 | 0.0067 | 0.0233 | 0.0245 | 0.0079 | 0.0041 | 0.0092 | 0.0106 | 0.0055 | 0.0269 |
Bud Periphery | 0.0066 | 0.0066 | 0.0192 | 0.0283 | 0.0087 | 0.0121 | 0.0034 | 0.0013 | 0.0059 | 0.0055 | 0.0131 | 0.0079 | 0.0017 | 0.0032 | 0.0082 | 0.0087 | 0.0056 | 0.007 |
Bud Site | 0.0179 | 0.0938 | 0.057 | 0.0032 | 0.0026 | 0.0033 | 0.0054 | 0.0643 | 0.0177 | 0.0064 | 0.0076 | 0.0008 | 0.0056 | 0.0868 | 0.0227 | 0.0027 | 0.0107 | 0.0012 |
Cell Periphery | 0.5454 | 0.387 | 0.4248 | 0.572 | 0.5023 | 0.5281 | 0.6927 | 0.6616 | 0.7047 | 0.6846 | 0.5331 | 0.7538 | 0.6989 | 0.535 | 0.6486 | 0.6567 | 0.7683 | 0.7054 |
Cytoplasm | 0.2181 | 0.4498 | 0.381 | 0.3461 | 0.2637 | 0.344 | 0.1864 | 0.2573 | 0.2283 | 0.1814 | 0.1616 | 0.1826 | 0.2199 | 0.3179 | 0.282 | 0.2491 | 0.1826 | 0.2349 |
Cytoplasmic Foci | 0.0085 | 0.0018 | 0.0048 | 0.0003 | 0.0089 | 0.0024 | 0.0096 | 0.0001 | 0.0032 | 0.0098 | 0.0108 | 0.0009 | 0.0012 | 0.0008 | 0.0003 | 0.0012 | 0.0001 | 0.0009 |
Eisosomes | 0.0059 | 0.0019 | 0.0032 | 0.0012 | 0.0023 | 0.001 | 0.0184 | 0.0014 | 0.001 | 0.015 | 0.0063 | 0.0011 | 0.0077 | 0.0014 | 0.0018 | 0.0015 | 0.0004 | 0.0005 |
Endoplasmic Reticulum | 0.0241 | 0.0141 | 0.0178 | 0.0166 | 0.0295 | 0.018 | 0.0119 | 0.008 | 0.0153 | 0.0246 | 0.0253 | 0.0125 | 0.0232 | 0.0282 | 0.0159 | 0.0244 | 0.0205 | 0.0115 |
Endosome | 0.022 | 0.0005 | 0.0014 | 0.0001 | 0.0241 | 0.0045 | 0.0181 | 0 | 0.0024 | 0.0025 | 0.0457 | 0.0003 | 0.001 | 0.0029 | 0 | 0.0008 | 0 | 0.0007 |
Golgi | 0.0153 | 0.0011 | 0.0029 | 0.0004 | 0.0111 | 0.0067 | 0.0064 | 0.0001 | 0.0025 | 0.0043 | 0.0308 | 0.0008 | 0.0012 | 0.0012 | 0.0003 | 0.0019 | 0.0001 | 0.0004 |
Lipid Particles | 0.0081 | 0.0018 | 0.0104 | 0.0011 | 0.0133 | 0.0034 | 0.0096 | 0.0005 | 0.002 | 0.0142 | 0.0069 | 0.0012 | 0.0024 | 0.004 | 0.0008 | 0.0033 | 0.0009 | 0.001 |
Mitochondria | 0.0055 | 0.0001 | 0.0015 | 0 | 0.0025 | 0.002 | 0.0091 | 0 | 0.0014 | 0.0091 | 0.052 | 0.0047 | 0.0034 | 0.0001 | 0 | 0.0113 | 0 | 0.0004 |
None | 0.0153 | 0.0178 | 0.0146 | 0.0016 | 0.0061 | 0.0043 | 0.0015 | 0.0003 | 0.0007 | 0.002 | 0.0012 | 0.0003 | 0.0052 | 0.0013 | 0.0038 | 0.0051 | 0.0005 | 0.0011 |
Nuclear Periphery | 0.0133 | 0.001 | 0.0008 | 0.0006 | 0.0061 | 0.0026 | 0.002 | 0.0004 | 0.0005 | 0.0009 | 0.0022 | 0.0003 | 0.0009 | 0.0011 | 0.0005 | 0.0006 | 0.0005 | 0.0018 |
Nucleolus | 0.0007 | 0.0002 | 0.0002 | 0.0001 | 0.0004 | 0.0004 | 0.0002 | 0 | 0.0001 | 0.0002 | 0.0002 | 0 | 0.0002 | 0.0001 | 0 | 0.0001 | 0 | 0.0001 |
Nucleus | 0.0083 | 0.0006 | 0.0006 | 0.0004 | 0.0011 | 0.001 | 0.0004 | 0.0002 | 0.0002 | 0.0003 | 0.0007 | 0.0002 | 0.0004 | 0.0005 | 0.0002 | 0.0003 | 0.0002 | 0.0005 |
Peroxisomes | 0.0034 | 0.0001 | 0.0016 | 0 | 0.0041 | 0.0003 | 0.0026 | 0 | 0.0024 | 0.0167 | 0.0269 | 0.0016 | 0.0062 | 0 | 0 | 0.0007 | 0 | 0 |
Punctate Nuclear | 0.0046 | 0.0002 | 0.0002 | 0 | 0.0053 | 0.0012 | 0.0009 | 0 | 0.0002 | 0.0002 | 0.0003 | 0 | 0.0003 | 0.0001 | 0 | 0.0001 | 0 | 0.0002 |
Vacuole | 0.004 | 0.0028 | 0.0042 | 0.004 | 0.0029 | 0.0029 | 0.0028 | 0.0014 | 0.0018 | 0.0034 | 0.0085 | 0.0024 | 0.0029 | 0.0037 | 0.0024 | 0.0028 | 0.0036 | 0.0028 |
Vacuole Periphery | 0.001 | 0.0001 | 0.0001 | 0.0001 | 0.0007 | 0.0003 | 0.0004 | 0 | 0.0001 | 0.0002 | 0.0073 | 0.0001 | 0.0002 | 0.0001 | 0 | 0.0002 | 0 | 0.0001 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 26.4587 | 22.0131 | 22.5905 | 27.8424 | 22.0773 | 21.4694 | 27.8218 | 29.2455 | 29.4474 | 29.1363 |
Translational Efficiency | 0.904 | 1.2233 | 1.2229 | 1.08 | 1.0835 | 1.0952 | 1.1656 | 1.0049 | 0.9161 | 0.8541 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
1843 | 538 | 118 | 817 | 1840 | 1369 | 159 | 141 | 3683 | 1907 | 277 | 958 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 813.50 | 1015.98 | 1288.08 | 1305.70 | 918.04 | 991.22 | 1310.63 | 1359.22 | 865.73 | 998.21 | 1301.02 | 1313.58 |
Standard Deviation | 93.10 | 143.19 | 146.56 | 218.00 | 122.09 | 140.58 | 166.12 | 192.11 | 120.49 | 141.76 | 158.48 | 215.22 |
Intensity Change Log 2 | — | 0.320658 | 0.663008 | 0.682609 | — | 0.110648 | 0.513632 | 0.566150 | — | 0.213129 | 0.585740 | 0.622037 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.007601 | 0.010187 | 0.054045 | 0.065553 | 0.007342 | 0.016529 | 0.062047 | 0.068590 | 0.007472 | 0.014740 | 0.058639 | 0.066000 |
Bud Neck | 0.071903 | 0.036077 | 0.058226 | 0.063279 | 0.047017 | 0.059086 | 0.045262 | 0.044699 | 0.059470 | 0.052595 | 0.050784 | 0.060544 |
Bud Site | 0.053082 | 0.113877* | 0.205924* | 0.234912* | 0.063062 | 0.096167 | 0.192874* | 0.272483* | 0.058068 | 0.101164* | 0.198433* | 0.240442* |
Cell Periphery | 0.184592* | 0.399865* | 0.166223* | 0.219159* | 0.244223* | 0.346218* | 0.157769* | 0.194333* | 0.214383* | 0.361353* | 0.161370* | 0.215505* |
Cytoplasm | 0.535663* | 0.382786* | 0.115300* | 0.154104* | 0.552256* | 0.397859* | 0.123301* | 0.146152* | 0.543953* | 0.393607* | 0.119893* | 0.152934* |
Cytoplasmic Foci | 0.014954 | 0.005725 | 0.029093 | 0.004574 | 0.008543 | 0.011067 | 0.026722 | 0.005458 | 0.011751 | 0.009560 | 0.027732 | 0.004704 |
Eisosomes | 0.050330 | 0.009590 | 0.009306 | 0.046872 | 0.025904 | 0.023309 | 0.023688 | 0.033167 | 0.038127 | 0.019439 | 0.017561 | 0.044855 |
Endoplasmic Reticulum | 0.009040 | 0.013171 | 0.115303* | 0.017544 | 0.009619 | 0.007600 | 0.051795 | 0.021321 | 0.009329 | 0.009171 | 0.078849 | 0.018100 |
Endosome | 0.001474 | 0.001157 | 0.008244 | 0.002704 | 0.001138 | 0.002010 | 0.004312 | 0.000830 | 0.001306 | 0.001770 | 0.005987 | 0.002428 |
Golgi | 0.005778 | 0.003562 | 0.005319 | 0.003680 | 0.004180 | 0.005532 | 0.001273 | 0.000339 | 0.004980 | 0.004976 | 0.002997 | 0.003189 |
Lipid Particles | 0.005077 | 0.002029 | 0.021762 | 0.009009 | 0.003421 | 0.002369 | 0.009400 | 0.003807 | 0.004249 | 0.002273 | 0.014666 | 0.008244 |
Mitochondria | 0.003585 | 0.001124 | 0.000270 | 0.000967 | 0.001518 | 0.001639 | 0.000124 | 0.000151 | 0.002552 | 0.001494 | 0.000186 | 0.000847 |
Mitotic Spindle | 0.000283 | 0.000264 | 0.000441 | 0.002206 | 0.000284 | 0.000580 | 0.000271 | 0.000145 | 0.000283 | 0.000491 | 0.000343 | 0.001903 |
None | 0.001896 | 0.000080 | 0.000049 | 0.000300 | 0.000507 | 0.000120 | 0.000130 | 0.002103 | 0.001202 | 0.000108 | 0.000095 | 0.000565 |
Nuclear Periphery | 0.000148 | 0.000138 | 0.000328 | 0.000217 | 0.000146 | 0.000120 | 0.000277 | 0.000157 | 0.000147 | 0.000125 | 0.000299 | 0.000208 |
Nuclear Periphery Foci | 0.000695 | 0.000917 | 0.009343 | 0.001052 | 0.000697 | 0.000609 | 0.007001 | 0.001573 | 0.000696 | 0.000696 | 0.007999 | 0.001129 |
Nucleolus | 0.000165 | 0.000040 | 0.000039 | 0.000028 | 0.000072 | 0.000080 | 0.000046 | 0.000016 | 0.000119 | 0.000069 | 0.000043 | 0.000026 |
Nucleus | 0.029791 | 0.003223 | 0.026065 | 0.054071 | 0.010801 | 0.006435 | 0.032422 | 0.037643 | 0.020304 | 0.005528 | 0.029714 | 0.051653 |
Peroxisomes | 0.006860 | 0.003397 | 0.000728 | 0.000477 | 0.004260 | 0.003339 | 0.000499 | 0.000308 | 0.005561 | 0.003355 | 0.000597 | 0.000452 |
Vacuole | 0.016753 | 0.012407 | 0.173754 | 0.117716 | 0.014727 | 0.018922 | 0.260704* | 0.164162 | 0.015741 | 0.017084 | 0.223664* | 0.124552 |
Vacuole Periphery | 0.000330 | 0.000384 | 0.000239 | 0.001574 | 0.000282 | 0.000412 | 0.000084 | 0.002562 | 0.000306 | 0.000404 | 0.000150 | 0.001720 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -2.59 | -7.10 | -17.90 | -16.67 | -2.10 | -6.84 | -6.31 | -7.19 | -6.02 | -0.96 | -7.24 | -9.07 | -19.38 | -16.30 | -2.09 |
Bud Neck | 8.40 | 1.68 | 2.50 | -5.89 | -0.62 | -3.35 | 1.11 | 1.68 | 4.09 | 0.20 | 2.38 | 2.36 | 0.31 | -1.72 | -2.04 |
Bud Site | -7.65 | -8.84 | -22.18 | -10.95 | -1.71 | -6.63 | -7.20 | -10.37 | -8.46 | -3.19 | -10.44 | -11.18 | -24.19 | -16.73 | -3.51 |
Cell Periphery | -15.08 | 1.62 | -2.78 | 12.54 | -2.85 | -10.62 | 6.67 | 4.63 | 9.72 | -1.01 | -18.91 | 5.82 | 1.67 | 16.69 | -4.32 |
Cytoplasm | 11.89 | 28.58 | 48.64 | 17.42 | -2.57 | 17.60 | 32.68 | 23.53 | 14.12 | -1.38 | 21.48 | 43.47 | 57.93 | 29.81 | -3.10 |
Cytoplasmic Foci | 7.25 | -3.65 | 7.71 | 0.61 | 5.93 | -1.81 | -4.88 | 1.43 | 2.48 | 5.16 | 1.95 | -5.92 | 7.07 | 3.58 | 7.98 |
Eisosomes | 21.82* | 21.25 | 1.85 | -13.66 | -13.63 | 1.69 | 1.86 | -0.36 | -0.97 | -1.40 | 14.41 | 11.96 | -1.86 | -9.99 | -10.03 |
Endoplasmic Reticulum | -1.98 | -7.25 | -5.81 | -1.99 | 6.62 | 1.71 | -6.49 | -3.26 | -3.86 | 4.04 | 0.15 | -9.17 | -6.77 | -6.30 | 7.84 |
Endosome | 1.00 | -6.75 | -1.29 | -1.67 | 4.54 | -2.59 | -5.45 | 1.01 | 2.94 | 5.58 | -1.82 | -8.44 | -1.40 | -0.71 | 4.32 |
Golgi | 2.09 | 0.95 | 1.84 | 0.10 | 1.15 | -1.52 | 6.83 | 9.07 | 6.60 | 4.22 | 0.00 | 4.95 | 1.89 | 1.68 | -0.17 |
Lipid Particles | 5.90 | -5.90 | -3.27 | -5.63 | 3.94 | 2.40 | -6.06 | -0.99 | -2.65 | 4.85 | 5.72 | -7.59 | -3.92 | -5.70 | 3.38 |
Mitochondria | 5.35 | 9.05 | 5.72 | 0.48 | -2.23 | -0.43 | 9.19 | 9.05 | 6.22 | -0.90 | 3.88 | 11.93 | 5.47 | 2.17 | -2.53 |
Mitotic Spindle | 0.34 | -2.25 | -1.75 | -1.76 | -1.61 | -1.13 | -1.10 | 2.20 | 1.59 | 1.50 | -1.11 | -1.41 | -1.72 | -1.44 | -1.40 |
None | 8.99 | 9.16 | 7.74 | -4.79 | -5.63 | 5.78 | 4.28 | -0.77 | -1.02 | -0.99 | 10.17 | 9.88 | 2.77 | -1.72 | -1.68 |
Nuclear Periphery | 0.52 | -5.34 | -5.75 | -3.58 | 3.15 | 2.31 | -4.51 | -1.30 | -2.68 | 3.73 | 2.27 | -6.79 | -6.08 | -6.54 | 3.96 |
Nuclear Periphery Foci | -0.83 | -7.99 | -4.41 | -0.81 | 7.55 | 1.09 | -7.86 | -3.03 | -3.23 | 6.20 | -0.05 | -11.17 | -5.48 | -4.09 | 10.28 |
Nucleolus | 6.36 | 6.75 | 7.55 | 1.56 | 2.66 | -0.31 | 1.83 | 15.84 | 1.93 | 2.12 | 1.83 | 6.13 | 9.91 | 1.81 | 2.11 |
Nucleus | 20.46 | 0.62 | -9.48 | -19.64 | -7.14 | 6.53 | -6.74 | -7.51 | -8.50 | -1.66 | 19.30 | -4.42 | -14.13 | -20.41 | -7.13 |
Peroxisomes | 6.12 | 14.19 | 15.16 | 7.62 | 2.50 | 1.78 | 9.96 | 10.64 | 8.35 | 2.39 | 5.58 | 17.28 | 18.09 | 10.28 | 2.31 |
Vacuole | 2.49 | -9.17 | -23.15 | -23.02 | 3.40 | -3.44 | -13.72 | -10.96 | -10.71 | 3.32 | -1.42 | -16.11 | -25.07 | -24.45 | 7.53 |
Vacuole Periphery | -0.59 | 1.61 | -1.27 | -1.21 | -1.38 | -1.62 | 5.08 | -0.88 | -0.81 | -0.98 | -1.53 | 4.49 | -1.52 | -1.39 | -1.72 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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