Standard name
Human Ortholog
Description Cytoplasmic protein containing a zinc finger domain; sequence similarity to that of Type I J-proteins; computational analysis of large-scale protein-protein interaction data suggests a possible role in actin patch assembly
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.95 0.97 0.96 0.96 0.92 0.89 0.88 0.87 0.83 0.78 0.65 0.69 0.98 0.96 0.96 0.74 0.68 0.7 0.9 0.94 0.94 0.92 0.86 0.84
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.09 0 0.07 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.16 0.05 0.12 0.27 0.26 0.45 0.35 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.12 0.1 0.1 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0.05 0.17 0 0.15 0.09 0.05 0.06 0.05 0.07 0 0 0 0.05 0.13 0.05 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 1 1 2 5 1 3 1 0 1 2 7 8
Bud 0 1 0 0 0 1 0 2 1 10 2 6 0 1 1 0 1 0 0 1 1 1 11 15
Bud Neck 0 0 0 1 0 0 0 0 0 1 1 3 0 0 0 0 0 0 0 0 0 1 2 1
Bud Site 0 0 0 0 0 0 0 1 0 0 1 2 0 0 0 0 1 0 – – – – – –
Cell Periphery 0 1 4 2 1 1 0 2 7 1 0 1 0 1 1 0 0 1 0 0 0 0 0 0
Cytoplasm 213 261 187 151 189 220 377 461 265 317 200 265 323 390 364 84 46 43 211 258 181 256 302 316
Endoplasmic Reticulum 2 0 3 1 0 0 0 0 0 0 1 0 0 0 2 2 2 3 0 0 0 1 2 2
Endosome 2 0 0 1 2 0 3 2 1 4 1 2 4 12 8 10 2 4 4 3 0 2 7 6
Golgi 0 0 0 0 1 0 1 0 0 0 0 0 0 0 0 1 1 0 4 0 0 0 0 2
Mitochondria 1 1 0 0 1 40 21 65 86 105 137 135 0 0 0 1 3 2 1 1 0 5 3 5
Nucleus 2 0 2 1 2 6 8 9 6 2 7 10 2 0 1 2 1 0 2 1 0 0 1 0
Nuclear Periphery 1 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 1 0 0 0 1 1 0
Nucleolus 0 0 0 0 0 0 1 0 0 0 2 0 0 0 0 0 2 1 0 0 0 0 0 1
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 1 1 0 0 1 2 0 0 0 0 0 0 0
SpindlePole 1 0 2 1 3 0 2 2 0 1 0 0 0 6 4 14 7 6 0 1 3 2 5 9
Vac/Vac Membrane 7 9 5 8 34 5 64 47 17 23 16 27 7 15 9 6 9 3 5 4 1 0 3 1
Unique Cell Count 225 269 195 158 205 248 428 532 321 408 306 384 331 407 381 114 68 61 235 276 193 279 354 379
Labelled Cell Count 229 273 203 166 233 273 477 591 383 464 368 453 338 426 392 126 78 67 235 276 193 279 354 379


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.3 6.0 7.0 6.3 7.0 4.4 5.6 5.4 4.4 4.7 4.2 4.4 6.5 6.8 6.7 8.3 8.0 8.3 5.3 5.8 6.1
Std Deviation (1e-4) 0.7 0.8 1.8 1.3 1.6 1.2 1.6 1.6 1.0 1.3 0.7 1.1 1.2 1.4 1.5 1.4 1.6 1.5 1.1 1.3 1.6
Intensity Change (Log2) – – – -0.16 -0.01 -0.69 -0.34 -0.38 -0.67 -0.6 -0.73 -0.66 -0.11 -0.05 -0.07 0.23 0.19 0.23 -0.4 -0.28 -0.2


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm -0.2 -1.6 -2.7 -3.1 -3.5 -4.5 -5.6 -7.9 -7.4 1.1 0 -0.2 -5.7 -6.3 -5.7
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 3.1 5.1 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 4.3 0 0
Vacuole 1.2 4.7 0 4.6 2.9 1.5 1.7 1.5 2.2 0 0.7 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 2.0386 1.6995 1.8619 0.7932 1.8503 1.539 3.7736 3.3667 3.3611 2.9488 2.8119 3.3679 6.1091 4.6413 4.9221 5.2599 5.1171 4.4373
Actin 0.0253 0.0098 0.0551 0.0003 0.007 0.0015 0.0496 0.0103 0.0239 0.0041 0.0045 0.0137 0.0002 0.0009 0.0551 0.1278 0.0001 0.0007
Bud 0.0005 0.0001 0.0013 0.0001 0.0034 0 0.0026 0.0011 0.0011 0.0076 0.0008 0.0003 0 0.0001 0.0006 0.001 0 0
Bud Neck 0.0007 0.0002 0.0067 0.0006 0.0009 0.001 0.0077 0.0019 0.0011 0.0006 0.0013 0.0024 0 0.0001 0.0004 0.0076 0.0002 0.0008
Bud Periphery 0.0008 0.0001 0.0016 0 0.0059 0 0.004 0.001 0.0015 0.004 0.0008 0.0006 0 0 0.0008 0.0015 0 0
Bud Site 0.0007 0.0013 0.0138 0.0001 0.004 0.0001 0.0109 0.0064 0.0021 0.002 0.0004 0.0002 0.0002 0.0008 0.0016 0.0129 0.0001 0
Cell Periphery 0.0002 0.0002 0.0006 0 0.0004 0 0.0004 0.0004 0.0002 0.0001 0.0001 0 0.0001 0.0001 0.0002 0.0017 0 0
Cytoplasm 0.6035 0.7025 0.6551 0.8534 0.6644 0.8309 0.6459 0.8458 0.7776 0.8614 0.6713 0.8449 0.4756 0.8347 0.7432 0.5605 0.8177 0.8375
Cytoplasmic Foci 0.0167 0.0042 0.0091 0.0027 0.0162 0.0049 0.0147 0.0057 0.0059 0.0065 0.0252 0.0174 0.003 0.0058 0.005 0.0075 0.0024 0.0045
Eisosomes 0.0002 0.0001 0.0007 0 0.0001 0 0.0003 0.0001 0.0003 0 0.0002 0 0.0001 0 0.0003 0.0009 0 0
Endoplasmic Reticulum 0.0139 0.0059 0.0117 0.0018 0.0152 0.0075 0.0134 0.0053 0.0055 0.0053 0.0042 0.0036 0.002 0.0054 0.0077 0.0052 0.0038 0.0038
Endosome 0.0304 0.0014 0.0155 0.0008 0.034 0.0052 0.0181 0.003 0.0097 0.0046 0.0105 0.0091 0.0006 0.0036 0.0096 0.007 0.0033 0.0018
Golgi 0.0038 0.0003 0.009 0 0.0029 0.0002 0.0064 0.0004 0.0015 0.0003 0.0013 0.0014 0 0.0003 0.0046 0.0084 0.0001 0.0001
Lipid Particles 0.0023 0.0003 0.0032 0 0.0023 0.0003 0.0113 0.0002 0.0037 0.0001 0.0069 0.0008 0.0001 0.0003 0.0015 0.0039 0.0001 0.0001
Mitochondria 0.0028 0.0001 0.0064 0.0001 0.0042 0.0002 0.0059 0.0002 0.0025 0.0005 0.0006 0.0016 0.0001 0.0002 0.0012 0.0011 0.0001 0.0001
None 0.216 0.2622 0.1839 0.1345 0.1198 0.1239 0.12 0.0997 0.1323 0.0769 0.2089 0.0601 0.4849 0.141 0.1519 0.2405 0.146 0.1424
Nuclear Periphery 0.0356 0.0015 0.0116 0.0008 0.034 0.0048 0.0362 0.0034 0.0073 0.0037 0.0057 0.0061 0.0027 0.0018 0.0053 0.0023 0.005 0.0015
Nucleolus 0.0015 0.0001 0.0004 0 0.0008 0.0001 0.001 0.0001 0.0003 0.0001 0.0015 0.0006 0.0001 0 0.0001 0.0002 0.0001 0
Nucleus 0.0265 0.0072 0.0067 0.0038 0.0574 0.0147 0.0309 0.0114 0.0086 0.0175 0.0127 0.0238 0.0285 0.0029 0.0071 0.0063 0.0182 0.0053
Peroxisomes 0.0004 0.0001 0.0018 0 0.0012 0.0002 0.008 0.0002 0.0061 0.0002 0.0017 0.0005 0 0.0001 0.0004 0.0015 0 0
Punctate Nuclear 0.0087 0.0013 0.0024 0.0006 0.0078 0.0036 0.0079 0.0019 0.0025 0.0023 0.04 0.0114 0.0012 0.0008 0.0016 0.0011 0.0017 0.0008
Vacuole 0.0067 0.001 0.0024 0.0003 0.0122 0.0008 0.0032 0.0013 0.0042 0.0018 0.0011 0.0011 0.0005 0.0008 0.0012 0.001 0.001 0.0006
Vacuole Periphery 0.0029 0.0001 0.0011 0.0001 0.006 0.0002 0.0017 0.0002 0.0023 0.0004 0.0003 0.0006 0 0.0001 0.0004 0.0003 0.0002 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 20.3307 9.9133 13.4862 22.9147 20.3688 25.8612 23.8055 18.604 27.0735 21.6177
Translational Efficiency 0.9453 1.5325 1.3603 0.9027 0.9331 1.27 1.4585 1.9531 1.3197 1.3329

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
2251 1787 2492 1729 2049 2747 2207 83 4300 4534 4699 1812

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 684.10 816.76 925.19 824.00 719.75 833.99 884.36 994.79 701.09 827.20 906.01 831.82
Standard Deviation 79.83 104.33 108.95 516.75 81.13 102.46 109.61 123.62 82.40 103.54 111.15 506.73
Intensity Change Log 2 — 0.255705 0.435542 0.268437 — 0.212534 0.297138 0.466896 — 0.233733 0.366242 0.373590

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000159 0.000147 0.000198 0.000423 0.000048 0.000299 0.000095 0.000592 0.000106 0.000239 0.000150 0.000431
Bud Neck 0.014094 0.006226 0.007502 0.004152 0.006337 0.016042 0.003864 0.001213 0.010398 0.012173 0.005793 0.004018
Bud Site 0.004798 0.004389 0.008825 0.006892 0.003699 0.012954 0.004318 0.002518 0.004275 0.009578 0.006708 0.006692
Cell Periphery 0.000242 0.000079 0.000072 0.000426 0.000101 0.000102 0.000062 0.000217 0.000175 0.000093 0.000068 0.000416
Cytoplasm 0.502267* 0.628994* 0.587543* 0.538985* 0.586402* 0.613477* 0.608170* 0.385175* 0.542358* 0.619593* 0.597231* 0.531940*
Cytoplasmic Foci 0.173698 0.089294 0.004478 0.036067 0.112247 0.074409 0.009502 0.027466 0.144416 0.080275 0.006838 0.035673
Eisosomes 0.000156 0.000037 0.000010 0.000105 0.000072 0.000048 0.000015 0.000100 0.000116 0.000044 0.000012 0.000105
Endoplasmic Reticulum 0.001209 0.001002 0.000773 0.001367 0.000423 0.000326 0.000926 0.005862 0.000835 0.000592 0.000845 0.001573
Endosome 0.003522 0.005041 0.000509 0.003278 0.003670 0.004420 0.000923 0.006607 0.003593 0.004665 0.000703 0.003431
Golgi 0.001589 0.000780 0.000190 0.000484 0.000838 0.000966 0.000145 0.002588 0.001231 0.000893 0.000169 0.000581
Lipid Particles 0.005145 0.001706 0.000239 0.001628 0.003118 0.001276 0.000345 0.003215 0.004179 0.001445 0.000289 0.001701
Mitochondria 0.004937 0.000902 0.001102 0.000654 0.001610 0.003422 0.000252 0.020483 0.003352 0.002429 0.000703 0.001562
Mitotic Spindle 0.000414 0.001756 0.005356 0.006895 0.002348 0.007215 0.001820 0.002387 0.001336 0.005063 0.003695 0.006688
None 0.004974 0.005415 0.004052 0.005811 0.003980 0.005777 0.005885 0.001048 0.004500 0.005634 0.004913 0.005592
Nuclear Periphery 0.000282 0.000340 0.001044 0.000770 0.000365 0.000299 0.000630 0.001429 0.000322 0.000315 0.000849 0.000801
Nuclear Periphery Foci 0.000259 0.000908 0.000185 0.000903 0.000309 0.000212 0.000692 0.003844 0.000282 0.000487 0.000423 0.001038
Nucleolus 0.001193 0.000644 0.000150 0.000735 0.000531 0.000749 0.000148 0.000180 0.000878 0.000708 0.000149 0.000709
Nucleus 0.250441* 0.205136* 0.356467* 0.318771* 0.241729* 0.216027* 0.326748* 0.295555* 0.246290* 0.211735* 0.342509* 0.317708*
Peroxisomes 0.003436 0.001547 0.000402 0.001135 0.001621 0.003177 0.000087 0.000864 0.002571 0.002535 0.000254 0.001122
Vacuole 0.026552 0.045023 0.020684 0.069762 0.030287 0.038268 0.035312 0.238369* 0.028331 0.040930 0.027554 0.077485
Vacuole Periphery 0.000633 0.000634 0.000220 0.000756 0.000264 0.000535 0.000062 0.000287 0.000457 0.000574 0.000146 0.000734

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 0.05 -0.47 -0.96 -0.99 -0.83 -2.06 -5.91 -4.49 -1.61 -3.76 -1.78 -1.58 -1.29 -0.66 -1.04
Bud Neck 6.68 4.97 9.35 2.80 4.14 -7.52 2.97 7.57 12.28 3.00 -1.87 5.72 9.11 9.77 2.86
Bud Site 0.44 -2.59 -1.71 -2.06 0.64 -6.98 -0.25 0.11 7.77 0.42 -6.33 -2.26 -2.19 2.19 -0.61
Cell Periphery 4.00 4.16 -2.65 -10.95 -11.08 0.07 8.44 -4.67 -4.63 -6.38 3.83 4.93 -5.70 -10.65 -11.59
Cytoplasm -15.37 -3.80 -1.79 12.07 1.61 -3.59 5.97 10.34 11.39 8.57 -13.82 1.28 4.50 14.49 3.56
Cytoplasmic Foci 16.74 42.10 32.40 16.12 -18.88 9.59 32.47 21.21 14.56 -2.28 19.88 52.32 36.76 19.68 -17.88
Eisosomes 11.17 13.76 5.17 -13.03 -19.88 6.93 19.08 -3.20 -5.45 -8.25 12.33 17.74 2.52 -12.45 -20.07
Endoplasmic Reticulum 0.69 0.17 -1.99 -3.74 -3.12 1.95 -11.40 -7.34 -7.45 -6.29 1.89 -3.44 -5.73 -8.06 -4.18
Endosome -2.67 11.70 0.52 2.83 -8.01 -1.64 7.91 -0.82 -0.14 -3.42 -3.10 13.44 0.64 3.07 -7.89
Golgi 3.65 8.04 4.98 1.38 -1.27 -0.43 2.93 -0.11 0.04 -0.96 1.78 7.29 3.46 1.97 -1.63
Lipid Particles 9.21 14.04 9.51 0.46 -9.73 5.22 7.75 0.03 -4.51 -6.67 10.71 15.54 8.94 -1.72 -10.23
Mitochondria 5.74 5.49 6.07 1.10 1.52 -2.45 3.40 -0.80 -0.54 -0.99 1.74 6.41 4.50 2.87 -0.64
Mitotic Spindle -2.22 -5.53 -4.26 -3.04 -0.30 -3.19 0.42 1.03 3.96 0.83 -4.18 -4.11 -3.49 -0.91 -1.45
None -0.23 1.52 -0.14 0.08 -1.50 -1.77 -2.40 4.90 6.49 14.77 -1.45 -0.63 -0.54 0.65 -0.21
Nuclear Periphery -3.03 -24.59 -14.94 -13.10 6.39 1.33 -13.49 -10.53 -10.82 -6.48 -0.08 -27.47 -15.24 -14.28 2.50
Nuclear Periphery Foci -8.03 0.09 -8.85 -0.53 -9.36 2.13 -6.44 -5.38 -5.48 -4.81 -5.22 -5.52 -10.18 -7.23 -7.30
Nucleolus 1.96 3.54 1.82 -0.10 -4.26 -1.88 1.17 5.04 7.51 0.94 1.23 3.67 1.43 0.59 -2.88
Nucleus 7.00 -21.58 -8.48 -14.35 10.70 4.17 -18.75 -2.21 -3.21 2.91 7.76 -28.58 -10.03 -15.38 10.67
Peroxisomes 5.21 8.81 4.91 1.17 -2.06 -3.50 5.64 3.56 6.78 -1.50 0.05 10.41 3.76 3.65 -2.53
Vacuole -9.60 -4.23 -18.40 -11.35 -15.51 -4.51 -9.93 -10.07 -9.77 -9.10 -10.11 -10.34 -19.72 -15.73 -14.75
Vacuole Periphery 0.09 3.86 -0.04 -0.08 -1.51 -2.06 7.69 1.37 2.53 -2.10 -1.09 5.27 -0.59 -0.19 -1.76
Standard name
Human Ortholog
Description Cytoplasmic protein containing a zinc finger domain; sequence similarity to that of Type I J-proteins; computational analysis of large-scale protein-protein interaction data suggests a possible role in actin patch assembly
Localization
Cell Percentages cytoplasm (62%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Hua1

Hua1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Hua1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available