Standard name
Human Ortholog
Description TFIID subunit, involved in RNA pol II transcription initiation; possesses in vitro histone acetyltransferase activity but its role in vivo appears to be minor; involved in promoter binding and G1/S progression; relocalizes to the cytosol in response to hypoxia
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0.08 – 0.06 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0 0.05 0 – 0 0 0.07 0.14 0.15 0.09 0.09 0.08 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0.12 0.56 0.59 0.5 0.49 0.64 0.55 0.68 – 0.69 0.72 0 0 0.18 0.08 0.05 0.14 0 0 0 0 0 0
Nucleus 0.91 0.91 0.91 0.83 0.89 0.82 0.74 0.74 0.72 – 0.68 0.68 0.91 0.82 0.76 0.83 0.75 0.81 0.83 0.82 0.76 0.81 0.76 0.68
Nuclear Periphery 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.17 0.18 0.18 0.14 0.08 0.11 0.15 0.13 0.17 – 0.21 0.26 0.16 0.08 0.13 0.1 0.11 0.1 0.12 0.1 0.16 0.15 0.14 0.22
Peroxisomes 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 1 1 1 3 4 2 – 2 3 0 0 3 0 1 1 0 2 1 0 1 3
Bud Neck 0 0 0 0 0 0 0 3 0 – 1 0 0 0 0 0 0 0 0 0 1 0 0 0
Bud Site 0 1 5 0 2 4 6 10 13 – 9 12 0 0 2 0 0 3 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 1 0 0 0 0 0 0 0
Cytoplasm 5 4 1 3 6 4 6 19 5 – 3 3 26 59 79 36 25 39 0 0 0 0 1 0
Endoplasmic Reticulum 0 0 0 0 0 1 0 0 0 – 1 0 0 2 1 2 3 5 0 0 0 0 2 1
Endosome 0 0 0 0 0 0 0 0 0 – 0 0 1 2 0 9 11 5 1 1 0 0 3 3
Golgi 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 1 1 0 0 2
Mitochondria 5 35 64 46 80 82 175 206 107 – 100 194 7 11 96 29 15 69 1 0 0 0 1 4
Nucleus 147 273 104 65 142 136 204 278 113 – 98 181 327 359 400 317 206 400 135 251 89 150 271 272
Nuclear Periphery 1 0 0 0 0 1 3 6 4 – 2 0 1 1 5 1 2 4 0 0 0 0 0 1
Nucleolus 28 54 20 11 13 19 40 50 27 – 30 69 59 34 71 37 30 50 20 29 18 27 50 87
Peroxisomes 0 0 0 0 0 0 0 0 1 – 0 0 0 1 3 1 0 1 0 0 0 0 0 0
SpindlePole 1 4 1 0 1 0 2 3 1 – 3 4 1 5 2 13 9 16 0 1 1 2 5 8
Vac/Vac Membrane 0 5 0 0 1 2 0 1 0 – 0 1 1 1 3 1 7 11 3 14 2 3 10 12
Unique Cell Count 162 301 114 78 159 166 275 374 157 144 268 361 436 526 381 274 493 164 305 118 187 356 403
Labelled Cell Count 187 376 195 126 246 250 439 580 273 249 467 423 475 665 446 310 604 164 305 118 187 356 403


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 6.3 5.6 4.0 4.1 4.3 3.9 3.5 3.8 3.4 – 3.3 3.2 6.3 5.9 5.0 7.7 8.1 8.0 5.8 5.8 6.4
Std Deviation (1e-4) 0.9 0.9 0.7 1.0 1.1 1.2 0.9 1.3 1.0 – 0.6 0.8 1.4 1.4 1.2 1.9 2.1 1.9 1.2 1.0 1.4
Intensity Change (Log2) – – – 0.04 0.08 -0.04 -0.19 -0.08 -0.25 – -0.28 -0.34 0.64 0.55 0.32 0.93 1.0 0.99 0.54 0.52 0.67


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 – 0 0 2.5 3.9 4.1 3.1 3.0 2.7
Endoplasmic Reticulum 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 – 0 0 -14.1 -14.9 -8.5 -11.6 -11.3 -9.8
Nucleus -1.7 -0.5 -2.2 -3.8 -3.8 -3.9 – -4.5 -4.9 -0.2 -2.3 -3.6 -2.1 -3.6 -2.6
Nuclear Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 – 0 0 -0.3 -3.1 -1.1 -2.3 -1.8 -2.2
Peroxisomes 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 3.4439 3.3474 2.9587 2.7267 2.9707 3.089 5.2688 4.4882 4.4866 3.7541 3.9963 3.8904 2.9396 2.7364 2.9761 3.2885 2.5312 2.9217
Actin 0.009 0 0.0176 0.0029 0.0559 0.0014 0.0526 0 0.0113 0.0063 0.0024 0.0018 0.0108 0.002 0 0.0148 0 0.0001
Bud 0.0003 0 0.0002 0.0001 0.0006 0 0.002 0 0.0003 0.0002 0.0002 0.0005 0.0004 0 0 0.0001 0 0.0001
Bud Neck 0.0025 0.0001 0.0002 0.0001 0.001 0.0013 0.0053 0 0.0008 0.0004 0.0008 0.0013 0.0021 0.0001 0.0002 0.0002 0.0005 0.0008
Bud Periphery 0.0008 0 0.0002 0.0001 0.0007 0.0001 0.0066 0 0.0005 0.0003 0.0006 0.0011 0.0005 0 0 0.0002 0.0001 0.0005
Bud Site 0.0019 0 0.0013 0.0002 0.0053 0.0001 0.0052 0 0.002 0.0019 0.001 0.0006 0.0052 0.0002 0.0006 0.0005 0.0001 0.0002
Cell Periphery 0.0003 0 0 0 0.0002 0 0.0008 0 0.0002 0.0001 0.0002 0.0002 0.0002 0 0 0 0 0.0001
Cytoplasm 0.0024 0.0001 0.0001 0.004 0.0013 0.0001 0.0369 0 0.002 0.0008 0.0006 0.0005 0.0117 0.0002 0.003 0.0002 0 0.0001
Cytoplasmic Foci 0.004 0 0.0025 0.0021 0.0034 0.0002 0.0224 0 0.009 0.004 0.0099 0.0004 0.0021 0.0017 0.0008 0.0026 0 0
Eisosomes 0.0004 0 0.0001 0 0.0002 0 0.0004 0 0.0001 0.0001 0.0001 0 0.0001 0 0 0.0001 0 0
Endoplasmic Reticulum 0.0015 0 0.0001 0.0018 0.0026 0.0001 0.0072 0 0.0019 0.0019 0.0007 0.0016 0.0037 0 0.0001 0.0005 0 0
Endosome 0.0077 0 0.0012 0.008 0.0096 0.0007 0.0351 0 0.015 0.0134 0.033 0.006 0.0064 0.0007 0.0002 0.0042 0 0.0001
Golgi 0.0026 0 0.0019 0.0006 0.0055 0.0006 0.0098 0 0.0054 0.0041 0.0191 0.0021 0.0018 0.0021 0 0.0042 0 0
Lipid Particles 0.0115 0 0.0027 0.001 0.0037 0.0019 0.0211 0 0.0049 0.0123 0.017 0.003 0.001 0.0082 0 0.007 0 0
Mitochondria 0.0111 0.0001 0.0005 0.0004 0.0017 0.0005 0.0244 0.0001 0.0077 0.0038 0.0078 0.01 0.0011 0.0007 0.0001 0.0078 0.0003 0.0011
None 0.0042 0 0.0002 0.0036 0.0012 0.0001 0.0088 0 0.0004 0.0006 0.0002 0.0001 0.0173 0.0001 0.0013 0.0004 0 0.0001
Nuclear Periphery 0.0252 0.0043 0.0003 0.0101 0.0026 0.0005 0.0444 0.0002 0.0063 0.0079 0.0012 0.005 0.0117 0.0006 0.0006 0.001 0.0007 0.0009
Nucleolus 0.0561 0.0248 0.0174 0.0176 0.1159 0.0904 0.0352 0.0217 0.0186 0.024 0.1047 0.0966 0.0392 0.0253 0.0486 0.0083 0.1243 0.0957
Nucleus 0.8199 0.9696 0.9464 0.9392 0.7819 0.8999 0.6234 0.9775 0.9029 0.9098 0.7917 0.8644 0.87 0.9516 0.938 0.945 0.8672 0.8983
Peroxisomes 0.0022 0 0.0065 0.0002 0.0024 0.0005 0.0229 0 0.0034 0.0018 0.0035 0.0004 0.0006 0.0044 0 0.0017 0 0.0001
Punctate Nuclear 0.0329 0.0008 0.0006 0.007 0.0033 0.0014 0.0175 0.0004 0.0032 0.0035 0.0018 0.0007 0.0111 0.002 0.0062 0.0008 0.0064 0.0013
Vacuole 0.0017 0 0 0.0004 0.0005 0.0001 0.0125 0 0.0026 0.0019 0.0026 0.0023 0.0022 0 0.0001 0.0002 0.0001 0.0003
Vacuole Periphery 0.0016 0 0 0.0004 0.0003 0.0001 0.0056 0 0.0013 0.0008 0.001 0.0013 0.0006 0 0 0.0003 0 0.0002

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 15.8565 16.948 13.9481 18.3645 18.7709 23.427 19.395 20.9235 18.6056 19.7191
Translational Efficiency 0.8959 0.6587 0.9538 0.7444 0.6306 0.5951 0.6207 0.5309 0.6058 0.6845

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description TFIID subunit, involved in RNA pol II transcription initiation; possesses in vitro histone acetyltransferase activity but its role in vivo appears to be minor; involved in promoter binding and G1/S progression; relocalizes to the cytosol in response to hypoxia
Localization
Cell Percentages nucleus (97%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-4

Taf1

Taf1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Taf1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available