ORF | |
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Human Ortholog | |
Description | Putative methyltransferase; may interact with ribosomes, based on co-purification experiments; predicted to be involved in ribosome biogenesis; null mutant is resistant to fluconazole; GFP-fusion protein localizes to the nucleolus; YGR283C has a paralog, YMR310C, that arose from the whole genome duplication |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 1 | 0 | 0 | 0 | 6 | 11 | 25 | 29 | 20 | 30 | 23 | 16 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 3 | 2 | 2 | 7 |
Bud Neck | 1 | 0 | 14 | 21 | 47 | 25 | 24 | 45 | 23 | 42 | 21 | 26 | 5 | 13 | 11 | 6 | 1 | 4 | 0 | 1 | 2 | 1 | 5 | 9 |
Bud Site | 0 | 0 | 2 | 7 | 9 | 19 | 38 | 36 | 32 | 36 | 24 | 26 | 0 | 0 | 0 | 8 | 2 | 5 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 5 | 16 | 11 | 2 | 24 | 22 | 29 | 46 | 35 | 36 | 26 | 31 | 10 | 47 | 56 | 13 | 14 | 18 | 0 | 5 | 2 | 1 | 1 | 1 |
Endoplasmic Reticulum | 0 | 0 | 1 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 3 | 4 | 3 | 0 | 0 | 0 | 0 | 1 | 0 |
Endosome | 0 | 7 | 9 | 10 | 15 | 15 | 11 | 5 | 14 | 9 | 4 | 13 | 8 | 22 | 32 | 13 | 13 | 14 | 9 | 9 | 22 | 14 | 23 | 31 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 3 | 2 | 0 | 3 | 0 |
Mitochondria | 37 | 17 | 8 | 11 | 10 | 57 | 100 | 85 | 139 | 109 | 151 | 101 | 8 | 0 | 1 | 28 | 14 | 29 | 2 | 1 | 3 | 2 | 4 | 2 |
Nucleus | 44 | 56 | 91 | 50 | 40 | 39 | 32 | 53 | 29 | 33 | 21 | 24 | 43 | 56 | 46 | 102 | 62 | 88 | 20 | 29 | 19 | 8 | 8 | 7 |
Nuclear Periphery | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 4 | 2 | 1 | 0 | 0 | 0 | 2 | 3 |
Nucleolus | 109 | 127 | 130 | 180 | 145 | 83 | 100 | 97 | 81 | 61 | 44 | 38 | 90 | 192 | 188 | 243 | 137 | 212 | 89 | 120 | 155 | 71 | 87 | 96 |
Peroxisomes | 0 | 0 | 0 | 0 | 1 | 7 | 4 | 1 | 6 | 5 | 6 | 7 | 0 | 0 | 1 | 5 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 0 |
SpindlePole | 23 | 39 | 144 | 219 | 262 | 216 | 247 | 323 | 294 | 287 | 151 | 218 | 77 | 196 | 217 | 142 | 68 | 83 | 32 | 27 | 62 | 78 | 109 | 103 |
Vac/Vac Membrane | 1 | 0 | 2 | 0 | 1 | 1 | 1 | 0 | 0 | 2 | 2 | 2 | 0 | 1 | 1 | 5 | 2 | 6 | 6 | 2 | 5 | 6 | 5 | 5 |
Unique Cell Count | 160 | 193 | 265 | 291 | 353 | 331 | 392 | 484 | 449 | 432 | 304 | 341 | 155 | 334 | 343 | 352 | 208 | 317 | 170 | 206 | 289 | 191 | 261 | 280 |
Labelled Cell Count | 221 | 263 | 412 | 501 | 561 | 497 | 611 | 720 | 673 | 650 | 473 | 502 | 243 | 528 | 554 | 569 | 321 | 465 | 170 | 206 | 289 | 191 | 261 | 280 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 5.1 | 6.7 | 6.2 | 5.3 | 5.3 | 4.1 | 3.8 | 4.2 | 3.6 | 3.8 | 3.3 | 3.7 | 5.8 | 6.1 | 6.0 | 7.3 | 7.8 | 7.5 | 6.1 | 6.4 | 6.5 |
Std Deviation (1e-4) | 0.9 | 1.3 | 1.6 | 1.1 | 2.0 | 1.3 | 1.4 | 1.4 | 1.1 | 1.2 | 1.1 | 0.8 | 1.2 | 1.2 | 1.2 | 1.7 | 1.9 | 2.0 | 1.2 | 1.7 | 1.9 |
Intensity Change (Log2) | – | – | – | -0.22 | -0.24 | -0.6 | -0.71 | -0.55 | -0.8 | -0.72 | -0.9 | -0.76 | -0.1 | -0.01 | -0.04 | 0.24 | 0.32 | 0.27 | -0.03 | 0.05 | 0.06 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0.9 | 3.3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | -1.0 | -0.8 | -1.3 | -2.5 | -3.0 | -2.8 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | -2.7 | 1.4 | 1.3 | 1.7 | 2.6 | 1.9 | 2.1 | 2.1 | 2.4 | 1.0 | 4.1 | 4.8 | -0.3 | 1.2 | 0.8 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0.5 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0.9 | 1.8 | 2.9 | 0.2 | 1.5 | 0.6 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0.5 | -0.1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1.1 | 0 | 0 | 2.6 | 1.9 | 3.0 |
Nucleus | -4.6 | -6.9 | -6.6 | -8.4 | -7.8 | -9.6 | -8.9 | -8.2 | -8.5 | -1.4 | -5.0 | -6.1 | -1.4 | -1.0 | -1.7 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 3.0 | -2.0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1.8 | 2.1 | 1.4 | 5.0 | 3.7 | 4.3 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 5.2 | 5.2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | -0.9 | 1.1 | 2.2 | -3.5 | -4.7 | -6.9 |
Vacuole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 1.0603 | 1.3707 | 1.2091 | 0.8416 | 0.6355 | 1.2235 | 1.7109 | 2.5486 | 2.4314 | 1.8222 | 1.983 | 2.0289 | 1.66 | 2.1786 | 1.7685 | 1.8579 | 1.4981 | 1.646 |
Actin | 0.0029 | 0 | 0.0032 | 0 | 0.0018 | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0.0002 | 0.0003 | 0.0001 | 0.0007 | 0.0011 | 0 | 0 | 0 | 0 | 0.0002 | 0 | 0 | 0 | 0 | 0 | 0 | 0.0001 | 0 |
Bud Neck | 0.0021 | 0.0013 | 0.0015 | 0.0022 | 0.0026 | 0.0013 | 0.0023 | 0.0026 | 0.0014 | 0.0148 | 0.0017 | 0.0012 | 0.0009 | 0.0008 | 0.0009 | 0.0007 | 0.0009 | 0.0011 |
Bud Periphery | 0.0006 | 0.0003 | 0.0001 | 0.0004 | 0.002 | 0.0001 | 0 | 0.0001 | 0 | 0.0004 | 0.0001 | 0 | 0.0001 | 0.0001 | 0 | 0.0001 | 0.0001 | 0 |
Bud Site | 0.0015 | 0.0057 | 0.0005 | 0.0007 | 0.0352 | 0.0005 | 0.0003 | 0.001 | 0.0002 | 0.0033 | 0.0002 | 0.0001 | 0.0003 | 0.0004 | 0.0003 | 0.0002 | 0.0005 | 0.0002 |
Cell Periphery | 0.0007 | 0.0003 | 0.0001 | 0.0004 | 0.0005 | 0.0001 | 0.0001 | 0.0002 | 0.0001 | 0.0011 | 0.0001 | 0 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0001 |
Cytoplasm | 0.002 | 0.0017 | 0.0002 | 0.0007 | 0.0008 | 0.0003 | 0 | 0.0001 | 0.0001 | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasmic Foci | 0.0056 | 0.0008 | 0.0023 | 0.0038 | 0.0067 | 0.0036 | 0.001 | 0.0007 | 0.0005 | 0.0009 | 0.0019 | 0.0015 | 0.0017 | 0.0005 | 0.0008 | 0.0005 | 0.0004 | 0.0018 |
Eisosomes | 0.0001 | 0 | 0 | 0 | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0.0018 | 0.0001 | 0.0002 | 0 | 0.0003 | 0.0001 | 0 | 0 | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0.0075 | 0.0006 | 0.0028 | 0.0003 | 0.0178 | 0.0012 | 0.0002 | 0.0006 | 0.0004 | 0.0018 | 0.0002 | 0.0001 | 0.0003 | 0.0004 | 0.0002 | 0.0001 | 0.0001 | 0.0002 |
Golgi | 0.0022 | 0.0001 | 0.0013 | 0.0002 | 0.0086 | 0.0004 | 0.0001 | 0.0001 | 0.0001 | 0.0002 | 0.0003 | 0.0002 | 0.0002 | 0.0001 | 0.0001 | 0 | 0.0001 | 0.0002 |
Lipid Particles | 0.0138 | 0.002 | 0.0152 | 0.0052 | 0.0158 | 0.0167 | 0.0036 | 0.0023 | 0.0015 | 0.0027 | 0.01 | 0.0073 | 0.0076 | 0.0015 | 0.0035 | 0.0007 | 0.0014 | 0.0072 |
Mitochondria | 0.0027 | 0.0001 | 0.0004 | 0.003 | 0.0088 | 0.0002 | 0.0001 | 0 | 0.0001 | 0.0001 | 0.0002 | 0 | 0.0002 | 0.0001 | 0.0001 | 0 | 0.0002 | 0 |
None | 0.0065 | 0.0001 | 0.0039 | 0.0008 | 0.0008 | 0.0001 | 0 | 0.0001 | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0.0062 | 0.001 | 0.0007 | 0.0005 | 0.0008 | 0.0044 | 0.0001 | 0.0001 | 0.0041 | 0.0001 | 0.0003 | 0.0001 | 0.0005 | 0.0061 | 0 | 0 | 0.0001 | 0.0009 |
Nucleolus | 0.7057 | 0.7352 | 0.8167 | 0.7368 | 0.6004 | 0.7451 | 0.8112 | 0.8373 | 0.8665 | 0.8122 | 0.7643 | 0.8134 | 0.8422 | 0.7951 | 0.8709 | 0.8184 | 0.7755 | 0.8159 |
Nucleus | 0.1324 | 0.2018 | 0.1168 | 0.1785 | 0.0663 | 0.0784 | 0.0935 | 0.117 | 0.1002 | 0.1316 | 0.0857 | 0.0807 | 0.0857 | 0.1603 | 0.1089 | 0.1512 | 0.11 | 0.0723 |
Peroxisomes | 0.0007 | 0.0001 | 0.0004 | 0.0006 | 0.0084 | 0.0007 | 0.0004 | 0.0001 | 0.0001 | 0.0004 | 0.0004 | 0.0002 | 0.0005 | 0.0001 | 0.0001 | 0 | 0.0002 | 0.0002 |
Punctate Nuclear | 0.0981 | 0.0427 | 0.0294 | 0.063 | 0.2182 | 0.1453 | 0.0869 | 0.0354 | 0.024 | 0.0138 | 0.1342 | 0.0947 | 0.0593 | 0.0324 | 0.0136 | 0.0277 | 0.1098 | 0.0997 |
Vacuole | 0.0057 | 0.0052 | 0.0038 | 0.0015 | 0.0024 | 0.001 | 0.0003 | 0.0021 | 0.0004 | 0.016 | 0.0003 | 0.0001 | 0.0004 | 0.0018 | 0.0005 | 0.0002 | 0.0004 | 0.0002 |
Vacuole Periphery | 0.001 | 0.0006 | 0.0005 | 0.0005 | 0.0005 | 0.0002 | 0 | 0.0001 | 0.0002 | 0.0003 | 0.0001 | 0 | 0.0001 | 0.0002 | 0 | 0 | 0.0001 | 0 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 28.4992 | 11.5366 | 16.4079 | 19.3238 | 20.2182 | 11.8233 | 16.0388 | 15.7456 | 10.1026 | 21.0979 |
Translational Efficiency | 0.9617 | 2.1365 | 1.2465 | 0.6514 | 1.0217 | 1.6693 | 1.016 | 0.7289 | 1.3686 | 0.9746 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
553 | 208 | 2443 | 1146 | 1414 | 1799 | 234 | 708 | 1967 | 2007 | 2677 | 1854 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 701.28 | 876.23 | 921.68 | 985.40 | 719.46 | 801.82 | 1006.74 | 913.53 | 714.35 | 809.53 | 929.12 | 957.95 |
Standard Deviation | 92.05 | 119.36 | 140.46 | 195.06 | 89.16 | 103.76 | 137.51 | 194.52 | 90.36 | 107.89 | 142.25 | 197.96 |
Intensity Change Log 2 | — | 0.321319 | 0.394275 | 0.490719 | — | 0.156364 | 0.484705 | 0.344538 | — | 0.240143 | 0.440777 | 0.418544 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000398 | 0.000966 | 0.000253 | 0.000584 | 0.000205 | 0.000375 | 0.000558 | 0.000770 | 0.000259 | 0.000436 | 0.000279 | 0.000655 |
Bud Neck | 0.004730 | 0.020831 | 0.008946 | 0.017727 | 0.005359 | 0.010362 | 0.008193 | 0.025926 | 0.005182 | 0.011447 | 0.008880 | 0.020858 |
Bud Site | 0.001497 | 0.004143 | 0.001269 | 0.004549 | 0.000955 | 0.001960 | 0.001666 | 0.017744 | 0.001107 | 0.002186 | 0.001304 | 0.009588 |
Cell Periphery | 0.000083 | 0.000091 | 0.000033 | 0.000134 | 0.000075 | 0.000102 | 0.000047 | 0.000886 | 0.000077 | 0.000101 | 0.000034 | 0.000421 |
Cytoplasm | 0.002936 | 0.004170 | 0.000622 | 0.002195 | 0.006024 | 0.006623 | 0.001045 | 0.052836 | 0.005156 | 0.006369 | 0.000659 | 0.021534 |
Cytoplasmic Foci | 0.005741 | 0.003300 | 0.000273 | 0.002094 | 0.004881 | 0.003148 | 0.000293 | 0.009831 | 0.005123 | 0.003164 | 0.000275 | 0.005049 |
Eisosomes | 0.000101 | 0.000126 | 0.000054 | 0.000090 | 0.000093 | 0.000099 | 0.000075 | 0.000112 | 0.000095 | 0.000102 | 0.000056 | 0.000098 |
Endoplasmic Reticulum | 0.002412 | 0.004710 | 0.000822 | 0.001090 | 0.001665 | 0.002735 | 0.002089 | 0.002378 | 0.001875 | 0.002940 | 0.000933 | 0.001582 |
Endosome | 0.003180 | 0.005450 | 0.000377 | 0.002156 | 0.003171 | 0.006045 | 0.000615 | 0.010256 | 0.003174 | 0.005984 | 0.000398 | 0.005249 |
Golgi | 0.002534 | 0.002898 | 0.000569 | 0.001165 | 0.002077 | 0.002261 | 0.000762 | 0.005373 | 0.002205 | 0.002327 | 0.000586 | 0.002772 |
Lipid Particles | 0.003377 | 0.001691 | 0.000084 | 0.000321 | 0.004156 | 0.002163 | 0.000208 | 0.003007 | 0.003937 | 0.002114 | 0.000095 | 0.001347 |
Mitochondria | 0.006363 | 0.002343 | 0.001069 | 0.005367 | 0.002952 | 0.010577 | 0.000878 | 0.006400 | 0.003911 | 0.009723 | 0.001052 | 0.005762 |
Mitotic Spindle | 0.010540 | 0.087933 | 0.034985 | 0.034361 | 0.016936 | 0.050060 | 0.033055 | 0.034057 | 0.015137 | 0.053985 | 0.034817 | 0.034245 |
None | 0.007196 | 0.001284 | 0.000648 | 0.001341 | 0.004709 | 0.005396 | 0.002102 | 0.026209 | 0.005408 | 0.004970 | 0.000775 | 0.010838 |
Nuclear Periphery | 0.000947 | 0.002187 | 0.000770 | 0.001924 | 0.000765 | 0.001866 | 0.000403 | 0.001004 | 0.000816 | 0.001899 | 0.000738 | 0.001573 |
Nuclear Periphery Foci | 0.020353 | 0.032221 | 0.003452 | 0.004354 | 0.021872 | 0.015515 | 0.005028 | 0.001364 | 0.021445 | 0.017246 | 0.003589 | 0.003212 |
Nucleolus | 0.622910* | 0.479120* | 0.600983* | 0.627545* | 0.680646* | 0.518170* | 0.567993* | 0.354071* | 0.664414* | 0.514123* | 0.598099* | 0.523112* |
Nucleus | 0.295614* | 0.329099* | 0.339929* | 0.275804* | 0.236345* | 0.352224* | 0.360288* | 0.419078* | 0.253008* | 0.349827* | 0.341709* | 0.330517* |
Peroxisomes | 0.007820 | 0.011836 | 0.003561 | 0.008086 | 0.006023 | 0.005222 | 0.008918 | 0.007144 | 0.006528 | 0.005907 | 0.004029 | 0.007726 |
Vacuole | 0.000746 | 0.004232 | 0.000635 | 0.005535 | 0.000639 | 0.003349 | 0.005218 | 0.015424 | 0.000669 | 0.003441 | 0.001036 | 0.009311 |
Vacuole Periphery | 0.000521 | 0.001371 | 0.000666 | 0.003579 | 0.000453 | 0.001748 | 0.000568 | 0.006130 | 0.000472 | 0.001709 | 0.000658 | 0.004553 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -3.57 | 2.08 | -2.89 | 2.37 | -8.05 | -5.13 | -5.91 | -10.70 | -7.49 | -1.19 | -4.76 | -1.68 | -10.92 | -5.92 | -10.95 |
Bud Neck | -5.47 | -5.93 | -10.99 | -0.09 | -7.66 | -7.14 | -3.75 | -12.71 | -10.46 | -9.51 | -9.08 | -7.64 | -16.86 | -11.31 | -12.23 |
Bud Site | -3.51 | -0.12 | -5.08 | -1.02 | -5.84 | -4.02 | -3.96 | -7.58 | -7.16 | -7.00 | -4.30 | -1.95 | -9.49 | -8.40 | -8.95 |
Cell Periphery | -0.67 | 5.17 | -2.69 | -2.12 | -5.58 | -2.14 | 2.21 | -3.55 | -3.41 | -3.69 | -2.17 | 6.05 | -4.02 | -3.70 | -4.64 |
Cytoplasm | -0.04 | 2.07 | 0.82 | 0.43 | -2.44 | -0.32 | 4.14 | -8.29 | -8.17 | -9.48 | -0.64 | 4.63 | -6.68 | -6.03 | -9.44 |
Cytoplasmic Foci | 2.27 | 4.28 | 3.16 | 1.46 | -4.03 | 3.01 | 7.47 | -1.44 | -3.16 | -5.23 | 3.74 | 8.05 | 1.88 | -1.64 | -6.17 |
Eisosomes | -2.67 | 11.03 | 3.21 | 4.81 | -9.46 | -1.06 | 3.79 | -1.64 | -1.14 | -3.74 | -1.33 | 16.40 | 0.76 | 1.80 | -10.85 |
Endoplasmic Reticulum | -5.26 | 8.62 | 5.78 | 8.22 | -3.44 | -7.40 | -3.05 | -4.07 | 0.53 | -0.15 | -7.67 | 10.13 | 0.94 | 7.56 | -6.62 |
Endosome | -1.78 | 3.53 | 1.19 | 2.68 | -4.46 | -3.35 | 7.23 | -3.65 | -1.70 | -5.70 | -3.70 | 8.55 | -1.89 | 1.60 | -7.14 |
Golgi | -0.68 | 5.17 | 3.52 | 4.75 | -3.16 | 0.27 | 6.45 | -1.23 | -1.28 | -2.23 | 0.45 | 9.54 | 0.26 | 0.06 | -2.96 |
Lipid Particles | 2.19 | 5.05 | 4.66 | 3.29 | -4.22 | 2.25 | 5.10 | 1.37 | -0.20 | -2.30 | 2.68 | 6.72 | 4.12 | 2.20 | -2.88 |
Mitochondria | 1.66 | 2.19 | 0.38 | -2.23 | -3.24 | -4.88 | 3.03 | -2.02 | 2.53 | -3.83 | -3.83 | 3.41 | -1.29 | 2.66 | -4.69 |
Mitotic Spindle | -6.40 | -6.96 | -5.93 | 4.19 | -0.58 | -8.08 | -2.72 | -4.86 | 1.10 | -0.21 | -10.40 | -7.30 | -7.20 | 3.00 | -0.94 |
None | 3.26 | 3.64 | 3.28 | 0.07 | -3.63 | -0.26 | 3.73 | -5.00 | -4.86 | -5.96 | 0.88 | 7.48 | -2.14 | -2.75 | -6.62 |
Nuclear Periphery | -1.61 | 0.50 | -1.63 | 0.19 | -2.06 | -4.18 | 2.72 | -3.66 | 2.18 | -6.34 | -4.20 | 0.28 | -2.51 | 0.47 | -2.48 |
Nuclear Periphery Foci | -1.90 | 6.12 | 5.61 | 4.83 | -0.94 | 3.25 | 5.93 | 11.40 | 10.95 | 1.62 | 2.41 | 11.45 | 11.60 | 10.13 | 0.32 |
Nucleolus | 8.62 | 1.90 | 0.63 | -8.57 | -1.34 | 20.38* | 7.76 | 25.42* | 13.32 | 9.97 | 21.03* | 10.44 | 17.67 | 0.51 | 10.11 |
Nucleus | -1.55 | -4.88 | 1.31 | 2.53 | 7.71 | -17.72 | -8.49 | -15.39 | -5.48 | -2.40 | -15.95 | -15.96 | -11.57 | 1.94 | 1.25 |
Peroxisomes | -1.65 | 4.72 | 0.63 | 2.23 | -11.02 | 1.70 | -1.50 | -0.23 | -1.71 | 1.16 | 1.66 | 5.63 | -0.46 | -2.55 | -8.65 |
Vacuole | -2.04 | 0.96 | -3.58 | -0.52 | -5.02 | -6.25 | -1.38 | -8.54 | -7.16 | -3.54 | -5.61 | -0.82 | -9.42 | -6.65 | -8.95 |
Vacuole Periphery | -4.64 | -1.15 | -6.61 | -4.37 | -6.06 | -6.48 | -1.89 | -4.64 | -3.37 | -4.48 | -7.01 | -1.76 | -7.66 | -4.89 | -7.00 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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