Standard name | |
---|---|
Human Ortholog | |
Description | Protein localized to COPII-coated vesicles; involved in vesicle formation and incorporation of specific secretory cargo; protein abundance increases in response to DNA replication stress |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 3 | 80 | 61 | 38 | 55 | 37 | 64 | 44 | 70 | 53 | 42 | 53 | 33 | 31 | 20 | 3 | 6 | – | 6 | 43 | 18 | 34 | 22 | 16 |
Bud | 0 | 5 | 8 | 4 | 2 | 17 | 21 | 17 | 25 | 22 | 5 | 8 | 1 | 1 | 1 | 0 | 2 | – | 1 | 5 | 0 | 1 | 2 | 9 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 1 | 4 | 0 | 3 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – | – |
Cell Periphery | 3 | 10 | 11 | 6 | 10 | 23 | 27 | 24 | 35 | 27 | 39 | 40 | 45 | 51 | 66 | 5 | 4 | – | 0 | 0 | 0 | 0 | 0 | 1 |
Cytoplasm | 20 | 152 | 42 | 45 | 109 | 139 | 187 | 163 | 152 | 130 | 111 | 123 | 38 | 35 | 50 | 15 | 5 | – | 5 | 17 | 2 | 8 | 7 | 14 |
Endoplasmic Reticulum | 16 | 484 | 253 | 159 | 202 | 202 | 340 | 317 | 207 | 248 | 172 | 235 | 347 | 415 | 421 | 1 | 1 | – | 39 | 502 | 282 | 276 | 239 | 317 |
Endosome | 0 | 1 | 5 | 3 | 2 | 2 | 10 | 3 | 5 | 7 | 2 | 3 | 0 | 0 | 0 | 0 | 0 | – | 3 | 21 | 5 | 15 | 4 | 8 |
Golgi | 6 | 45 | 33 | 44 | 35 | 7 | 16 | 18 | 10 | 9 | 7 | 9 | 47 | 26 | 22 | 4 | 8 | – | 6 | 54 | 31 | 53 | 39 | 29 |
Mitochondria | 0 | 4 | 5 | 0 | 3 | 1 | 8 | 5 | 11 | 12 | 18 | 27 | 0 | 0 | 0 | 0 | 0 | – | 0 | 3 | 3 | 8 | 3 | 3 |
Nucleus | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 2 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 1 | 1 | 0 | 1 | 0 | 3 | 2 | 3 | 5 | 4 | 3 | 0 | 0 | 0 | 0 | 0 | – | 0 | 2 | 1 | 2 | 0 | 0 |
Nucleolus | 4 | 1 | 0 | 1 | 2 | 4 | 5 | 7 | 5 | 5 | 2 | 3 | 0 | 0 | 1 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 2 | 0 | 1 | 0 | 0 |
Vac/Vac Membrane | 2 | 6 | 3 | 6 | 7 | 3 | 26 | 34 | 17 | 45 | 7 | 20 | 1 | 9 | 16 | 2 | 4 | – | 1 | 6 | 1 | 5 | 5 | 9 |
Unique Cell Count | 45 | 624 | 325 | 233 | 342 | 376 | 588 | 555 | 443 | 464 | 335 | 441 | 418 | 471 | 506 | 27 | 25 | 72 | 670 | 349 | 414 | 333 | 417 | |
Labelled Cell Count | 55 | 789 | 422 | 306 | 428 | 435 | 707 | 637 | 540 | 565 | 410 | 524 | 512 | 569 | 597 | 30 | 30 | 72 | 670 | 349 | 414 | 333 | 417 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 24.4 | 25.0 | 17.2 | 17.1 | 17.8 | 17.1 | 16.7 | 16.8 | 17.6 | 15.4 | 16.6 | 16.1 | 26.9 | 27.9 | 28.8 | 31.9 | 31.3 | – | 25.8 | 26.9 | 27.6 |
Std Deviation (1e-4) | 11.4 | 5.1 | 3.4 | 3.1 | 3.1 | 3.2 | 3.6 | 3.6 | 4.1 | 4.0 | 4.5 | 4.4 | 6.4 | 6.2 | 6.3 | 9.2 | 8.0 | – | 8.6 | 9.8 | 6.5 |
Intensity Change (Log2) | – | – | – | -0.01 | 0.05 | -0.01 | -0.04 | -0.03 | 0.04 | -0.16 | -0.05 | -0.09 | 0.65 | 0.7 | 0.74 | 0.89 | 0.87 | – | 0.59 | 0.65 | 0.68 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | -0.8 | -0.9 | -3.4 | -3.3 | -4.8 | -1.1 | -2.9 | -2.2 | -2.6 | -4.4 | -5.3 | -7.0 | 0 | 0 | – |
Bud | -0.6 | 0 | 1.5 | 0.9 | 0.5 | 2.1 | 1.6 | -0.9 | -0.6 | 0 | 0 | 0 | 0 | 0 | – |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – |
Cell Periphery | -0.5 | -0.3 | 1.7 | 0.9 | 0.7 | 2.6 | 1.6 | 4.0 | 3.1 | 3.8 | 3.9 | 4.7 | 0 | 0 | – |
Cytoplasm | 2.1 | 5.8 | 7.3 | 6.3 | 5.6 | 6.7 | 5.1 | 6.2 | 5.0 | -1.7 | -2.6 | -1.4 | 0 | 0 | – |
Endoplasmic Reticulum | -2.5 | -5.2 | -6.7 | -6.1 | -6.2 | -8.7 | -7.0 | -7.1 | -7.0 | 1.8 | 3.9 | 1.9 | -8.3 | -8.0 | – |
Endosome | 0 | 0 | 0 | 0.2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – |
Golgi | 2.9 | 0 | -4.7 | -4.8 | -4.2 | -4.7 | -5.1 | -4.3 | -4.9 | 0.5 | -2.5 | -3.3 | 0 | 0 | – |
Mitochondria | 0 | 0 | 0 | 0 | 0 | 0.9 | 1.0 | 2.7 | 3.1 | 0 | 0 | 0 | 0 | 0 | – |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – |
Vacuole | 0 | 0 | 0 | 2.9 | 3.7 | 2.5 | 5.1 | 0 | 2.9 | 0 | 0 | 2.1 | 0 | 0 | – |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 22.5679 | 21.1745 | 21.6261 | 22.0955 | 21.9925 | 22.2984 | 23.7809 | 26.5855 | 24.0848 | 21.5448 | 19.8819 | 25.3906 | 25.4918 | 27.2119 | 25.2591 | 24.5852 | 25.079 | 25.4975 |
Actin | 0.0042 | 0.0086 | 0.0109 | 0.0078 | 0.0016 | 0.0089 | 0.0269 | 0.0092 | 0.0199 | 0.0022 | 0.0553 | 0.0091 | 0.0062 | 0.0066 | 0.0015 | 0.0003 | 0.0044 | 0.0068 |
Bud | 0.0001 | 0.0002 | 0.0011 | 0.0001 | 0 | 0.0001 | 0.0002 | 0.0001 | 0.0001 | 0.0003 | 0.0006 | 0.0001 | 0 | 0.0002 | 0.0001 | 0 | 0 | 0.0001 |
Bud Neck | 0.0003 | 0.0008 | 0.0012 | 0.0008 | 0.0009 | 0.0012 | 0.0003 | 0.0005 | 0.0022 | 0.0006 | 0.0006 | 0.001 | 0.0002 | 0.0003 | 0.0003 | 0.0001 | 0.0004 | 0.0027 |
Bud Periphery | 0.0002 | 0.0002 | 0.001 | 0.0003 | 0.0001 | 0.0001 | 0.0004 | 0.0001 | 0.0001 | 0.0001 | 0.0008 | 0.0001 | 0.0001 | 0.0004 | 0.0002 | 0 | 0 | 0.0002 |
Bud Site | 0.0004 | 0.0022 | 0.001 | 0.0002 | 0 | 0.0001 | 0.0005 | 0.0011 | 0.0003 | 0.0014 | 0.0023 | 0.0002 | 0.0002 | 0.0015 | 0.0001 | 0 | 0 | 0.0001 |
Cell Periphery | 0.0019 | 0.0076 | 0.0038 | 0.0041 | 0.001 | 0.001 | 0.0011 | 0.0028 | 0.0012 | 0.0003 | 0.0006 | 0.0008 | 0.0023 | 0.0067 | 0.0032 | 0.0009 | 0.0006 | 0.0013 |
Cytoplasm | 0.0575 | 0.1884 | 0.0771 | 0.0883 | 0.0306 | 0.0551 | 0.0238 | 0.0607 | 0.0205 | 0.0471 | 0.0156 | 0.0136 | 0.0302 | 0.0573 | 0.0289 | 0.0101 | 0.0225 | 0.0178 |
Cytoplasmic Foci | 0.0096 | 0.0189 | 0.0043 | 0.0016 | 0.0028 | 0.0178 | 0.0175 | 0.0268 | 0.0096 | 0.0208 | 0.0145 | 0.0135 | 0.0036 | 0.0121 | 0.0052 | 0.0009 | 0.0117 | 0.0102 |
Eisosomes | 0.0002 | 0.0002 | 0.0001 | 0.0001 | 0 | 0.0002 | 0.0003 | 0.0004 | 0.0002 | 0 | 0.0002 | 0.0001 | 0.0001 | 0.0003 | 0.0002 | 0 | 0 | 0.0001 |
Endoplasmic Reticulum | 0.7747 | 0.5581 | 0.8312 | 0.8551 | 0.8984 | 0.6511 | 0.7552 | 0.7231 | 0.8269 | 0.8594 | 0.7421 | 0.6114 | 0.8145 | 0.7604 | 0.8472 | 0.9722 | 0.7837 | 0.6023 |
Endosome | 0.0274 | 0.0434 | 0.0098 | 0.0053 | 0.0102 | 0.0621 | 0.0376 | 0.0356 | 0.0185 | 0.0341 | 0.0342 | 0.0542 | 0.0214 | 0.0179 | 0.0114 | 0.0024 | 0.0329 | 0.0509 |
Golgi | 0.0464 | 0.0507 | 0.0135 | 0.009 | 0.0055 | 0.1156 | 0.0643 | 0.089 | 0.0486 | 0.0031 | 0.0207 | 0.2492 | 0.0534 | 0.0505 | 0.03 | 0.0059 | 0.064 | 0.2434 |
Lipid Particles | 0.0394 | 0.0875 | 0.0296 | 0.011 | 0.0322 | 0.062 | 0.0503 | 0.0419 | 0.0313 | 0.0042 | 0.0249 | 0.035 | 0.0376 | 0.0725 | 0.0595 | 0.0014 | 0.0544 | 0.0519 |
Mitochondria | 0.0021 | 0.0005 | 0.0004 | 0.0001 | 0 | 0.0013 | 0.0018 | 0.0004 | 0.0045 | 0.0011 | 0.0037 | 0.001 | 0.0031 | 0.0012 | 0.0006 | 0.0001 | 0.0003 | 0.0021 |
None | 0.0015 | 0.0005 | 0.0011 | 0.0002 | 0.0001 | 0.0002 | 0.001 | 0.0002 | 0.0002 | 0.0007 | 0.004 | 0.0001 | 0.0003 | 0.0004 | 0.0002 | 0 | 0.0002 | 0.0004 |
Nuclear Periphery | 0.0215 | 0.0167 | 0.0078 | 0.0125 | 0.0128 | 0.0168 | 0.0107 | 0.004 | 0.007 | 0.0125 | 0.0382 | 0.0067 | 0.0201 | 0.0062 | 0.008 | 0.0031 | 0.0212 | 0.0056 |
Nucleolus | 0.0004 | 0.0002 | 0.0001 | 0 | 0 | 0 | 0.0001 | 0.0001 | 0 | 0.0001 | 0.0008 | 0 | 0 | 0.0004 | 0 | 0 | 0 | 0 |
Nucleus | 0.003 | 0.003 | 0.0022 | 0.001 | 0.001 | 0.0011 | 0.0008 | 0.0003 | 0.0003 | 0.0069 | 0.0112 | 0.0002 | 0.0009 | 0.0006 | 0.0003 | 0.0001 | 0.0003 | 0.0004 |
Peroxisomes | 0.0002 | 0.0002 | 0.0001 | 0 | 0 | 0.0003 | 0.002 | 0.0004 | 0.0059 | 0.0014 | 0.0051 | 0.0002 | 0.0001 | 0.0002 | 0.0001 | 0 | 0.0001 | 0.0001 |
Punctate Nuclear | 0.0029 | 0.001 | 0.0004 | 0.0003 | 0.0002 | 0.0005 | 0.0021 | 0.0003 | 0.0004 | 0.0013 | 0.0197 | 0.0003 | 0.0002 | 0.0003 | 0.0001 | 0 | 0.0005 | 0.0002 |
Vacuole | 0.0042 | 0.0102 | 0.0029 | 0.0019 | 0.0023 | 0.0029 | 0.0021 | 0.0026 | 0.0015 | 0.0017 | 0.0033 | 0.0017 | 0.0039 | 0.0034 | 0.0023 | 0.0022 | 0.0014 | 0.0019 |
Vacuole Periphery | 0.0019 | 0.001 | 0.0004 | 0.0003 | 0.0003 | 0.0017 | 0.0011 | 0.0005 | 0.0006 | 0.0006 | 0.0016 | 0.0014 | 0.0017 | 0.0006 | 0.0006 | 0.0003 | 0.0013 | 0.0014 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 100.5245 | 83.5195 | 86.2944 | 135.7342 | 84.334 | 44.9155 | 113.308 | 128.4207 | 123.7126 | 117.1992 |
Translational Efficiency | 1.349 | 1.6926 | 2.0069 | 1.0915 | 1.4079 | 3.235 | 1.5716 | 1.4602 | 1.6354 | 1.4144 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✔ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
737 | 1539 | 2562 | 1259 | 1964 | 1596 | 2337 | 2339 | 2701 | 3135 | 4899 | 3598 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 1686.49 | 1596.09 | 2006.21 | 3077.07 | 1600.02 | 1602.08 | 2388.03 | 2880.86 | 1623.61 | 1599.14 | 2188.35 | 2949.52 |
Standard Deviation | 282.98 | 353.92 | 364.05 | 816.13 | 319.19 | 363.43 | 426.82 | 643.30 | 312.12 | 358.80 | 438.84 | 714.74 |
Intensity Change Log 2 | — | -0.079482 | 0.250449 | 0.867533 | — | 0.001856 | 0.577731 | 0.848410 | — | -0.039310 | 0.419057 | 0.858255 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.002145 | 0.005858 | 0.002742 | 0.002294 | 0.003845 | 0.007599 | 0.004667 | 0.002265 | 0.003381 | 0.006744 | 0.003660 | 0.002275 |
Bud Neck | 0.000142 | 0.000951 | 0.000970 | 0.003091 | 0.000568 | 0.000875 | 0.001451 | 0.000854 | 0.000451 | 0.000912 | 0.001199 | 0.001637 |
Bud Site | 0.000940 | 0.005495 | 0.003658 | 0.010964 | 0.003976 | 0.006217 | 0.005068 | 0.006603 | 0.003147 | 0.005863 | 0.004330 | 0.008129 |
Cell Periphery | 0.002023 | 0.002337 | 0.003155 | 0.035904 | 0.002076 | 0.003127 | 0.004326 | 0.022810 | 0.002061 | 0.002739 | 0.003714 | 0.027392 |
Cytoplasm | 0.009000 | 0.006054 | 0.006431 | 0.009544 | 0.006172 | 0.006089 | 0.006307 | 0.015648 | 0.006943 | 0.006072 | 0.006372 | 0.013512 |
Cytoplasmic Foci | 0.017678 | 0.041702 | 0.025103 | 0.006469 | 0.028119 | 0.037241 | 0.014400 | 0.014122 | 0.025270 | 0.039431 | 0.019997 | 0.011444 |
Eisosomes | 0.000280 | 0.000290 | 0.000194 | 0.000323 | 0.000246 | 0.000283 | 0.000262 | 0.000378 | 0.000255 | 0.000286 | 0.000227 | 0.000358 |
Endoplasmic Reticulum | 0.734613* | 0.462444* | 0.490156* | 0.604726* | 0.682322* | 0.505407* | 0.555955* | 0.596671* | 0.696590* | 0.484316* | 0.521544* | 0.599490* |
Endosome | 0.052012 | 0.106459 | 0.079597 | 0.022735 | 0.066915 | 0.111713 | 0.065270 | 0.023191 | 0.062848 | 0.109133 | 0.072762 | 0.023031 |
Golgi | 0.059385 | 0.125133* | 0.127312* | 0.032759 | 0.078001 | 0.102103 | 0.082145 | 0.040849 | 0.072921 | 0.113408* | 0.105766 | 0.038018 |
Lipid Particles | 0.097391 | 0.206763* | 0.217687* | 0.204070* | 0.097982 | 0.191470* | 0.222522* | 0.237433* | 0.097821 | 0.198978* | 0.219994* | 0.225759* |
Mitochondria | 0.001025 | 0.000626 | 0.000507 | 0.002091 | 0.000707 | 0.001795 | 0.000704 | 0.001710 | 0.000794 | 0.001221 | 0.000601 | 0.001843 |
Mitotic Spindle | 0.000068 | 0.000812 | 0.000515 | 0.003482 | 0.000398 | 0.000819 | 0.000413 | 0.000352 | 0.000308 | 0.000816 | 0.000466 | 0.001447 |
None | 0.012314 | 0.001382 | 0.002830 | 0.001731 | 0.002422 | 0.001049 | 0.001384 | 0.003686 | 0.005122 | 0.001213 | 0.002140 | 0.003002 |
Nuclear Periphery | 0.000247 | 0.000310 | 0.000573 | 0.002822 | 0.000336 | 0.000327 | 0.000646 | 0.001047 | 0.000312 | 0.000318 | 0.000608 | 0.001668 |
Nuclear Periphery Foci | 0.003195 | 0.012078 | 0.013218 | 0.010833 | 0.010631 | 0.008236 | 0.009682 | 0.005365 | 0.008602 | 0.010122 | 0.011531 | 0.007278 |
Nucleolus | 0.000013 | 0.000119 | 0.000008 | 0.000033 | 0.000008 | 0.000013 | 0.000006 | 0.000023 | 0.000009 | 0.000065 | 0.000007 | 0.000027 |
Nucleus | 0.000105 | 0.000275 | 0.000105 | 0.000481 | 0.000110 | 0.000174 | 0.000106 | 0.000310 | 0.000109 | 0.000224 | 0.000105 | 0.000370 |
Peroxisomes | 0.002407 | 0.000896 | 0.000746 | 0.000692 | 0.000759 | 0.000762 | 0.000538 | 0.004466 | 0.001208 | 0.000828 | 0.000647 | 0.003145 |
Vacuole | 0.003879 | 0.016053 | 0.021059 | 0.037997 | 0.010738 | 0.011099 | 0.021774 | 0.019269 | 0.008867 | 0.013531 | 0.021400 | 0.025822 |
Vacuole Periphery | 0.001141 | 0.003964 | 0.003435 | 0.006959 | 0.003670 | 0.003603 | 0.002376 | 0.002947 | 0.002980 | 0.003780 | 0.002930 | 0.004351 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -4.05 | -0.91 | -0.47 | 3.60 | 0.39 | -3.21 | -0.98 | 1.62 | 4.70 | 2.76 | -4.21 | -0.55 | 1.41 | 5.83 | 2.26 |
Bud Neck | -6.10 | -5.29 | -7.85 | -5.62 | -5.40 | -1.95 | -4.48 | -2.37 | -0.12 | 3.13 | -3.97 | -5.54 | -7.56 | -4.77 | -2.51 |
Bud Site | -6.74 | -5.94 | -6.61 | -3.70 | -4.80 | -2.73 | -1.74 | -2.96 | -0.68 | -1.20 | -4.63 | -2.62 | -6.28 | -3.11 | -4.47 |
Cell Periphery | -1.09 | -3.75 | -15.24 | -15.15 | -14.76 | -2.06 | -7.28 | -17.24 | -15.14 | -14.99 | -2.36 | -8.28 | -22.88 | -21.73 | -21.22 |
Cytoplasm | 2.53 | 2.37 | -0.63 | -2.41 | -2.23 | -0.04 | -0.32 | -7.66 | -7.65 | -7.65 | 1.07 | 0.62 | -6.78 | -7.72 | -7.70 |
Cytoplasmic Foci | -9.01 | -3.49 | 5.90 | 16.31 | 13.33 | -3.72 | 7.82 | 7.68 | 11.02 | 0.17 | -7.64 | 3.77 | 9.84 | 18.26 | 8.53 |
Eisosomes | -0.21 | 2.65 | -0.86 | -0.73 | -3.32 | -0.91 | -0.40 | -3.15 | -2.73 | -3.38 | -0.99 | 1.01 | -3.10 | -2.65 | -5.44 |
Endoplasmic Reticulum | 17.39 | 17.34 | 8.62 | -9.63 | -8.78 | 14.23 | 11.40 | 7.99 | -7.47 | -3.84 | 22.06 | 20.46 | 11.14 | -12.33 | -9.51 |
Endosome | -12.30 | -7.49 | 8.39 | 26.42 | 28.13* | -12.35 | 0.65 | 20.42 | 28.53 | 21.99* | -16.96 | -4.70 | 21.79 | 39.03 | 36.15* |
Golgi | -10.66 | -12.04 | 5.65 | 18.53 | 22.03* | -4.70 | -0.83 | 9.94 | 14.29 | 11.55 | -10.11 | -8.96 | 11.67 | 22.97 | 23.98* |
Lipid Particles | -11.55 | -14.32 | -10.55 | 0.25 | 1.51 | -12.71 | -18.02 | -19.75 | -5.41 | -1.80 | -17.82 | -24.08 | -22.24 | -4.16 | -0.95 |
Mitochondria | 1.80 | 2.83 | -1.45 | -1.97 | -2.15 | -1.48 | 0.02 | -3.38 | 0.09 | -2.75 | -1.07 | 0.95 | -3.20 | -1.35 | -3.75 |
Mitotic Spindle | -3.22 | -6.20 | -3.69 | -2.81 | -3.20 | -1.66 | -0.42 | 0.08 | 1.72 | 0.53 | -2.88 | -2.14 | -3.48 | -1.80 | -2.97 |
None | 4.43 | 3.81 | 4.20 | -0.61 | 1.38 | 2.30 | 1.85 | -1.49 | -3.69 | -3.34 | 4.88 | 3.65 | 2.33 | -3.50 | -1.63 |
Nuclear Periphery | -1.19 | -5.67 | -13.89 | -13.83 | -12.28 | 0.22 | -5.09 | -10.53 | -10.39 | -4.81 | -0.22 | -7.39 | -17.04 | -17.20 | -12.91 |
Nuclear Periphery Foci | -7.01 | -10.64 | -7.31 | 0.63 | 1.82 | 1.88 | 0.53 | 4.32 | 2.99 | 5.59 | -1.48 | -3.40 | 1.15 | 3.18 | 6.24 |
Nucleolus | -1.33 | 0.75 | -2.91 | 1.05 | -6.06 | -1.44 | 1.55 | -6.01 | -2.90 | -7.24 | -1.42 | 1.09 | -6.38 | 0.95 | -9.09 |
Nucleus | -1.27 | -0.27 | -3.57 | -1.61 | -3.56 | -1.26 | -0.13 | -5.47 | -2.29 | -5.50 | -1.63 | -0.16 | -5.74 | -2.12 | -5.76 |
Peroxisomes | 3.00 | 3.26 | 3.41 | 1.09 | 0.25 | -0.03 | 1.67 | -6.74 | -6.71 | -7.22 | 2.13 | 3.14 | -4.98 | -6.33 | -6.83 |
Vacuole | -8.44 | -13.42 | -12.55 | -8.04 | -6.35 | -0.31 | -7.63 | -6.53 | -6.13 | 1.86 | -4.86 | -12.74 | -13.09 | -9.44 | -3.12 |
Vacuole Periphery | -4.99 | -9.51 | -6.37 | -2.88 | -3.82 | 0.14 | 3.55 | 1.80 | 1.27 | -2.06 | -1.88 | 0.09 | -3.33 | -1.25 | -3.82 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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