Standard name | |
---|---|
Human Ortholog | |
Description | Endosomal protein involved in turnover of plasma membrane proteins; member of the DUP380 subfamily of conserved, often subtelomeric COS genes; required for the multivesicular vesicle body sorting pathway that internalizes plasma membrane proteins for degradation; Cos proteins provide ubiquitin in trans for nonubiquitinated cargo proteins |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 1 | 2 | 6 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 2 |
Bud | 0 | 0 | 0 | 1 | 2 | 0 | 4 | 3 | 1 | 3 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 1 | 2 | 3 | 4 | 4 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 11 | 0 | 53 | 97 | 106 | 221 | 313 | 293 | 199 | 239 | 263 | 261 | 141 | 253 | 281 | 21 | 25 | 5 | 2 | 0 | 0 | 1 | 6 | 8 |
Endoplasmic Reticulum | 2 | 0 | 1 | 0 | 7 | 6 | 5 | 0 | 1 | 2 | 3 | 4 | 15 | 65 | 31 | 2 | 2 | 1 | 1 | 1 | 7 | 1 | 5 | 5 |
Endosome | 1 | 0 | 11 | 8 | 15 | 3 | 2 | 15 | 2 | 0 | 0 | 0 | 33 | 40 | 52 | 0 | 1 | 0 | 3 | 0 | 7 | 9 | 20 | 22 |
Golgi | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 4 | 3 | 1 | 1 | 0 | 1 | 0 | 0 | 1 |
Mitochondria | 12 | 0 | 3 | 4 | 5 | 9 | 23 | 19 | 15 | 21 | 31 | 22 | 1 | 0 | 1 | 5 | 4 | 0 | 2 | 0 | 0 | 1 | 3 | 4 |
Nucleus | 22 | 2 | 29 | 24 | 22 | 42 | 76 | 93 | 43 | 62 | 22 | 35 | 5 | 8 | 6 | 6 | 4 | 1 | 2 | 0 | 6 | 5 | 10 | 11 |
Nuclear Periphery | 3 | 0 | 0 | 0 | 1 | 1 | 2 | 0 | 2 | 4 | 1 | 3 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 4 | 1 | 11 | 11 |
Nucleolus | 2 | 0 | 1 | 0 | 1 | 0 | 2 | 4 | 1 | 1 | 2 | 1 | 0 | 0 | 0 | 2 | 1 | 0 | 0 | 0 | 1 | 0 | 2 | 2 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 |
SpindlePole | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 2 | 7 | 6 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 1 | 2 |
Vac/Vac Membrane | 108 | 19 | 183 | 245 | 249 | 338 | 499 | 577 | 354 | 413 | 337 | 313 | 171 | 168 | 136 | 34 | 53 | 12 | 140 | 59 | 230 | 214 | 358 | 422 |
Unique Cell Count | 142 | 20 | 251 | 305 | 349 | 492 | 755 | 821 | 494 | 594 | 526 | 518 | 315 | 475 | 447 | 60 | 82 | 16 | 157 | 64 | 266 | 244 | 432 | 503 |
Labelled Cell Count | 161 | 21 | 282 | 380 | 408 | 621 | 926 | 1007 | 621 | 747 | 660 | 639 | 369 | 543 | 521 | 74 | 93 | 21 | 157 | 64 | 266 | 244 | 432 | 503 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 8.3 | 9.7 | 10.8 | 9.2 | 10.8 | 9.7 | 9.3 | 9.1 | 8.5 | 8.0 | 7.5 | 7.7 | 9.7 | 9.2 | 7.9 | 10.9 | 12.7 | 14.2 | 10.4 | 11.2 | 12.7 |
Std Deviation (1e-4) | 2.2 | 2.5 | 3.5 | 2.3 | 3.0 | 3.0 | 3.0 | 3.1 | 2.8 | 2.4 | 2.4 | 2.5 | 2.0 | 1.8 | 1.7 | 5.1 | 6.4 | 7.2 | 3.2 | 3.7 | 4.6 |
Intensity Change (Log2) | – | – | – | -0.24 | -0.0 | -0.16 | -0.21 | -0.25 | -0.34 | -0.43 | -0.53 | -0.48 | -0.15 | -0.23 | -0.46 | 0.02 | 0.23 | 0.39 | -0.05 | 0.05 | 0.23 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 2.8 | 2.5 | 6.4 | 5.8 | 4.3 | 5.2 | 5.3 | 7.7 | 7.7 | 5.9 | 8.3 | 10.6 | 2.3 | 1.7 | 0 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3.1 | 5.9 | 4.0 | 0 | 0 | 0 |
Endosome | -1.1 | -0.1 | 0 | 0 | -2.3 | 0 | 0 | 0 | 0 | 2.7 | 2.0 | 3.2 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | 1.6 | 1.1 | 1.5 | 1.9 | 3.0 | 2.2 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | -1.5 | -2.3 | -1.3 | -0.7 | -0.1 | -1.2 | -0.5 | -3.9 | -2.3 | -5.0 | -5.8 | -5.9 | -0.3 | -1.8 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 2.1 | -0.4 | -1.2 | -2.0 | -0.8 | -0.4 | -1.0 | -2.4 | -3.4 | -4.5 | -9.6 | -10.8 | -2.5 | -1.4 | 0.2 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 3.8374 | 6.7303 | 5.774 | 5.2023 | 4.871 | 5.623 | 6.0315 | 9.647 | 7.9508 | 6.9218 | 7.1653 | 8.0447 | 4.8981 | 8.8662 | 6.7854 | 7.2597 | 7.426 | 6.4717 |
Actin | 0.0021 | 0 | 0.0111 | 0.0047 | 0.0171 | 0.0013 | 0.003 | 0 | 0.0026 | 0 | 0.0017 | 0.0017 | 0.0087 | 0 | 0.0002 | 0.0044 | 0.0028 | 0 |
Bud | 0.0003 | 0.0005 | 0.0003 | 0.0005 | 0.0006 | 0.0003 | 0.0004 | 0.0003 | 0.0004 | 0.0007 | 0.0002 | 0.0004 | 0.0004 | 0.0002 | 0.0004 | 0.0005 | 0.0005 | 0.0005 |
Bud Neck | 0.0007 | 0.0012 | 0.0014 | 0.0011 | 0.0033 | 0.0011 | 0.0008 | 0.0014 | 0.0017 | 0.0014 | 0.0014 | 0.002 | 0.0016 | 0.0025 | 0.0015 | 0.0014 | 0.0014 | 0.0018 |
Bud Periphery | 0.0013 | 0.0019 | 0.0013 | 0.0014 | 0.0019 | 0.0017 | 0.0014 | 0.0013 | 0.0014 | 0.0015 | 0.0007 | 0.0021 | 0.0016 | 0.0009 | 0.0015 | 0.0007 | 0.0013 | 0.0021 |
Bud Site | 0.0009 | 0.0013 | 0.0009 | 0.0011 | 0.0028 | 0.0006 | 0.0011 | 0.0005 | 0.0024 | 0.0011 | 0.0006 | 0.001 | 0.0011 | 0.0007 | 0.001 | 0.0069 | 0.0012 | 0.0011 |
Cell Periphery | 0.009 | 0.0133 | 0.011 | 0.0085 | 0.0085 | 0.0096 | 0.0095 | 0.0164 | 0.0114 | 0.0057 | 0.0092 | 0.0144 | 0.0101 | 0.0098 | 0.0088 | 0.0046 | 0.0097 | 0.0076 |
Cytoplasm | 0.0027 | 0.0025 | 0.0055 | 0.0019 | 0.0021 | 0.0018 | 0.004 | 0.0023 | 0.0037 | 0.0014 | 0.0009 | 0.0025 | 0.0028 | 0.0012 | 0.0021 | 0.0021 | 0.0016 | 0.0021 |
Cytoplasmic Foci | 0.0153 | 0.0009 | 0.0032 | 0.0194 | 0.015 | 0.0125 | 0.0062 | 0.0008 | 0.0039 | 0.0038 | 0.0084 | 0.0019 | 0.0029 | 0.0009 | 0.003 | 0.0168 | 0.0111 | 0.0012 |
Eisosomes | 0 | 0 | 0.0001 | 0 | 0.0002 | 0 | 0.0001 | 0 | 0.0001 | 0 | 0 | 0 | 0.0004 | 0 | 0 | 0.0001 | 0 | 0 |
Endoplasmic Reticulum | 0.0082 | 0.0194 | 0.0124 | 0.0078 | 0.0027 | 0.0078 | 0.0067 | 0.0056 | 0.0184 | 0.0037 | 0.0055 | 0.0084 | 0.0087 | 0.0094 | 0.0111 | 0.0018 | 0.0023 | 0.0051 |
Endosome | 0.1507 | 0.0559 | 0.1111 | 0.1314 | 0.0965 | 0.1008 | 0.1123 | 0.037 | 0.096 | 0.087 | 0.0771 | 0.0543 | 0.0808 | 0.0289 | 0.0994 | 0.0825 | 0.0405 | 0.041 |
Golgi | 0.0068 | 0.0013 | 0.0057 | 0.017 | 0.0098 | 0.0091 | 0.0059 | 0.001 | 0.0053 | 0.0036 | 0.0125 | 0.0022 | 0.0046 | 0.0023 | 0.0062 | 0.0079 | 0.0034 | 0.0011 |
Lipid Particles | 0.0265 | 0.0023 | 0.0088 | 0.0127 | 0.0165 | 0.0215 | 0.0173 | 0.003 | 0.0211 | 0.0181 | 0.0396 | 0.0079 | 0.0224 | 0.0083 | 0.0142 | 0.0294 | 0.0305 | 0.0059 |
Mitochondria | 0.0298 | 0.0019 | 0.0158 | 0.0388 | 0.003 | 0.0153 | 0.012 | 0.0029 | 0.0159 | 0.0046 | 0.0107 | 0.0016 | 0.008 | 0.0014 | 0.0106 | 0.0045 | 0.0011 | 0.0014 |
None | 0.0011 | 0.0001 | 0.0019 | 0.0004 | 0.0012 | 0.0075 | 0.002 | 0 | 0.0017 | 0.0001 | 0.0002 | 0.0006 | 0.0018 | 0 | 0.0001 | 0.0016 | 0.0006 | 0.0001 |
Nuclear Periphery | 0.0108 | 0.0141 | 0.0195 | 0.0057 | 0.028 | 0.0101 | 0.0215 | 0.0111 | 0.0184 | 0.0027 | 0.0031 | 0.01 | 0.0223 | 0.0163 | 0.0204 | 0.0028 | 0.0041 | 0.0149 |
Nucleolus | 0.0033 | 0.0025 | 0.0025 | 0.0038 | 0.0083 | 0.0021 | 0.016 | 0.0029 | 0.0047 | 0.0052 | 0.0024 | 0.0069 | 0.0121 | 0.0048 | 0.0028 | 0.0043 | 0.0074 | 0.0179 |
Nucleus | 0.0059 | 0.0045 | 0.0075 | 0.0023 | 0.0052 | 0.0034 | 0.0196 | 0.005 | 0.0051 | 0.0036 | 0.0021 | 0.0095 | 0.0099 | 0.007 | 0.0034 | 0.0024 | 0.0045 | 0.0112 |
Peroxisomes | 0.0049 | 0.0001 | 0.0008 | 0.024 | 0.0035 | 0.0006 | 0.0096 | 0.0001 | 0.0011 | 0.001 | 0.0201 | 0.0004 | 0.0011 | 0.0001 | 0.0003 | 0.0043 | 0.0034 | 0.0002 |
Punctate Nuclear | 0.0053 | 0 | 0.0028 | 0.0007 | 0.0052 | 0.0003 | 0.0022 | 0 | 0.0011 | 0.0001 | 0.0006 | 0.0006 | 0.0018 | 0 | 0.0001 | 0.009 | 0.0043 | 0.0013 |
Vacuole | 0.5618 | 0.7502 | 0.6358 | 0.5711 | 0.673 | 0.645 | 0.58 | 0.7689 | 0.6186 | 0.7127 | 0.6915 | 0.7282 | 0.58 | 0.708 | 0.6601 | 0.6765 | 0.7161 | 0.7172 |
Vacuole Periphery | 0.1524 | 0.126 | 0.1407 | 0.1457 | 0.0957 | 0.1474 | 0.1682 | 0.1394 | 0.1651 | 0.1421 | 0.1116 | 0.1437 | 0.2172 | 0.1972 | 0.1527 | 0.1354 | 0.1523 | 0.1665 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 139.1291 | 123.9426 | 97.7189 | 93.7248 | 111.1034 | 139.5332 | 110.3061 | 104.8456 | 122.7943 | 126.7949 |
Translational Efficiency | 0.4348 | 0.3712 | 0.3934 | 0.4355 | 0.4129 | 0.5605 | 0.5017 | 0.4169 | 0.4636 | 0.4594 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
1684 | 417 | 2254 | 272 | 1485 | 1254 | 175 | 348 | 3169 | 1671 | 2429 | 620 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 991.62 | 960.39 | 1130.99 | 1085.27 | 874.46 | 983.97 | 1190.91 | 975.64 | 936.72 | 978.09 | 1135.31 | 1023.74 |
Standard Deviation | 170.78 | 183.59 | 168.92 | 238.12 | 183.22 | 152.39 | 172.99 | 211.15 | 186.14 | 161.07 | 169.93 | 229.91 |
Intensity Change Log 2 | — | -0.046167 | 0.189727 | 0.130195 | — | 0.170222 | 0.445600 | 0.157957 | — | 0.059285 | 0.315295 | 0.143271 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000015 | 0.000105 | 0.000219 | 0.002724 | 0.000063 | 0.000054 | 0.000335 | 0.001318 | 0.000037 | 0.000067 | 0.000228 | 0.001935 |
Bud Neck | 0.002018 | 0.003882 | 0.004873 | 0.012573 | 0.005128 | 0.004052 | 0.007236 | 0.009608 | 0.003475 | 0.004010 | 0.005044 | 0.010909 |
Bud Site | 0.003176 | 0.009917 | 0.005183 | 0.032028 | 0.008179 | 0.003336 | 0.006088 | 0.020187 | 0.005520 | 0.004978 | 0.005248 | 0.025381 |
Cell Periphery | 0.002466 | 0.002692 | 0.000449 | 0.003759 | 0.003082 | 0.003708 | 0.001277 | 0.002136 | 0.002755 | 0.003455 | 0.000509 | 0.002848 |
Cytoplasm | 0.008267 | 0.017354 | 0.016850 | 0.067723 | 0.021520 | 0.004415 | 0.035221 | 0.167289* | 0.014477 | 0.007644 | 0.018173 | 0.123609* |
Cytoplasmic Foci | 0.004050 | 0.013646 | 0.012617 | 0.030534 | 0.009632 | 0.002767 | 0.034479 | 0.058095 | 0.006666 | 0.005482 | 0.014192 | 0.046004 |
Eisosomes | 0.000007 | 0.000022 | 0.000017 | 0.000130 | 0.000014 | 0.000022 | 0.000083 | 0.000112 | 0.000011 | 0.000022 | 0.000022 | 0.000120 |
Endoplasmic Reticulum | 0.018288 | 0.009116 | 0.155156* | 0.141526* | 0.024689 | 0.021735 | 0.168897* | 0.158255* | 0.021288 | 0.018586 | 0.156146* | 0.150916* |
Endosome | 0.022288 | 0.046990 | 0.165222 | 0.096529 | 0.036290 | 0.020198 | 0.098822 | 0.060933 | 0.028849 | 0.026884 | 0.160438 | 0.076549 |
Golgi | 0.001094 | 0.003390 | 0.002713 | 0.005643 | 0.001730 | 0.001409 | 0.001080 | 0.003182 | 0.001392 | 0.001903 | 0.002595 | 0.004262 |
Lipid Particles | 0.000554 | 0.005269 | 0.019483 | 0.036785 | 0.002754 | 0.001163 | 0.033600 | 0.030606 | 0.001585 | 0.002188 | 0.020500 | 0.033316 |
Mitochondria | 0.000244 | 0.001029 | 0.000618 | 0.000234 | 0.000819 | 0.002906 | 0.000158 | 0.000452 | 0.000514 | 0.002438 | 0.000585 | 0.000357 |
Mitotic Spindle | 0.000026 | 0.000117 | 0.001249 | 0.001050 | 0.000156 | 0.000113 | 0.000759 | 0.003147 | 0.000087 | 0.000114 | 0.001214 | 0.002227 |
None | 0.000167 | 0.000164 | 0.000141 | 0.000212 | 0.000788 | 0.000551 | 0.000104 | 0.000401 | 0.000458 | 0.000455 | 0.000138 | 0.000318 |
Nuclear Periphery | 0.000353 | 0.001314 | 0.017197 | 0.003418 | 0.001161 | 0.000943 | 0.019526 | 0.007443 | 0.000731 | 0.001036 | 0.017365 | 0.005678 |
Nuclear Periphery Foci | 0.000452 | 0.003101 | 0.030216 | 0.029969 | 0.001484 | 0.000773 | 0.053290 | 0.057343 | 0.000936 | 0.001354 | 0.031879 | 0.045333 |
Nucleolus | 0.000436 | 0.002355 | 0.000971 | 0.000301 | 0.002028 | 0.001423 | 0.001183 | 0.000558 | 0.001182 | 0.001655 | 0.000986 | 0.000445 |
Nucleus | 0.000958 | 0.005567 | 0.005759 | 0.031966 | 0.005921 | 0.004809 | 0.020155 | 0.036288 | 0.003284 | 0.004998 | 0.006796 | 0.034392 |
Peroxisomes | 0.000190 | 0.001629 | 0.000631 | 0.001124 | 0.000504 | 0.000447 | 0.002229 | 0.000924 | 0.000337 | 0.000742 | 0.000746 | 0.001012 |
Vacuole | 0.873091* | 0.800768* | 0.540396* | 0.496507* | 0.814861* | 0.899063* | 0.510483* | 0.377563* | 0.845804* | 0.874534* | 0.538241* | 0.429745* |
Vacuole Periphery | 0.061859 | 0.071571 | 0.020038 | 0.005264 | 0.059196 | 0.026112 | 0.004997 | 0.004160 | 0.060611 | 0.037457 | 0.018954 | 0.004645 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -3.07 | -4.20 | -1.95 | -1.88 | -1.80 | 0.75 | -4.25 | -1.89 | -1.90 | -1.62 | -2.60 | -4.15 | -2.72 | -2.69 | -2.49 |
Bud Neck | -3.00 | -6.34 | -3.96 | -3.21 | -2.84 | 1.34 | -1.20 | -3.12 | -3.95 | -1.04 | -1.12 | -3.33 | -5.27 | -4.81 | -4.00 |
Bud Site | -4.11 | -3.33 | -4.98 | -3.74 | -4.61 | 4.36 | 1.13 | -3.16 | -4.31 | -3.47 | 0.60 | 0.12 | -5.95 | -6.03 | -5.97 |
Cell Periphery | -0.34 | 7.25 | -1.17 | -0.78 | -3.69 | -1.26 | 3.92 | 1.91 | 2.63 | -1.27 | -1.74 | 11.37 | 0.17 | 1.38 | -4.79 |
Cytoplasm | -2.38 | -4.78 | -7.81 | -6.20 | -6.69 | 6.66 | -2.14 | -11.99 | -13.60 | -9.40 | 3.81 | -2.28 | -14.04 | -14.93 | -13.47 |
Cytoplasmic Foci | -4.12 | -10.26 | -9.82 | -4.87 | -6.52 | 5.77 | -5.14 | -10.14 | -11.69 | -3.40 | 1.21 | -8.39 | -13.21 | -13.41 | -10.52 |
Eisosomes | -3.46 | -8.34 | -4.24 | -3.67 | -3.89 | -3.68 | -5.02 | -5.59 | -5.09 | -1.18 | -6.26 | -7.38 | -6.81 | -6.04 | -6.06 |
Endoplasmic Reticulum | 4.62 | -26.54 | -8.25 | -8.90 | 1.49 | 1.19 | -8.03 | -9.60 | -9.85 | 0.84 | 1.58 | -27.36 | -12.73 | -12.99 | 1.32 |
Endosome | -4.99 | -32.03* | -8.18 | -4.64 | 7.69 | 6.25 | -5.94 | -4.27 | -7.48 | 3.43 | 0.95 | -31.24 | -9.28 | -9.40 | 13.96 |
Golgi | -2.29 | -3.73 | -2.71 | -1.06 | -1.68 | 0.63 | 1.44 | -1.32 | -1.59 | -1.99 | -1.13 | -3.49 | -3.01 | -2.27 | -1.61 |
Lipid Particles | -2.51 | -14.50 | -6.33 | -5.20 | -2.92 | 2.94 | -5.68 | -7.69 | -8.21 | 0.43 | -1.10 | -14.59 | -9.85 | -9.57 | -3.70 |
Mitochondria | -2.48 | -5.94 | 0.02 | 2.47 | 5.29 | -2.09 | 4.04 | 1.88 | 2.49 | -2.07 | -2.57 | -0.87 | 1.52 | 2.79 | 2.70 |
Mitotic Spindle | -1.87 | -4.24 | -2.87 | -2.70 | -0.36 | 0.44 | -1.07 | -1.98 | -2.01 | -1.53 | -0.51 | -4.14 | -2.67 | -2.64 | -1.28 |
None | 0.00 | 0.23 | -0.47 | -0.56 | -0.94 | 0.74 | 2.89 | 1.62 | 0.79 | -3.24 | -0.05 | 2.64 | 1.06 | 0.93 | -3.09 |
Nuclear Periphery | -1.56 | -11.60 | -4.41 | -2.27 | 8.58 | 0.57 | -3.55 | -5.67 | -6.05 | 2.34 | -1.12 | -11.84 | -7.21 | -6.58 | 7.72 |
Nuclear Periphery Foci | -2.51 | -15.68 | -7.82 | -6.87 | 0.09 | 2.83 | -6.81 | -8.95 | -9.07 | -0.27 | -1.38 | -16.72 | -11.41 | -11.28 | -2.90 |
Nucleolus | -2.24 | -2.24 | 1.18 | 2.39 | 2.76 | 0.87 | 1.07 | 2.35 | 2.36 | 1.82 | -1.08 | 0.39 | 2.43 | 3.51 | 2.39 |
Nucleus | -2.59 | -5.71 | -4.87 | -4.14 | -4.14 | 0.77 | -2.29 | -6.92 | -7.08 | -2.20 | -1.75 | -3.76 | -8.26 | -7.70 | -7.16 |
Peroxisomes | -2.25 | -2.99 | -4.95 | 0.72 | -2.26 | 0.39 | -2.23 | -2.61 | -2.54 | 1.50 | -2.08 | -2.85 | -5.24 | -1.51 | -1.76 |
Vacuole | 6.00 | 45.49* | 20.61 | 14.31 | 2.29 | -11.05 | 13.31 | 25.76 | 31.36* | 4.61 | -4.81 | 44.66* | 33.05 | 34.19 | 7.87 |
Vacuole Periphery | -1.63 | 16.12 | 21.62 | 12.27 | 13.97 | 10.55 | 19.82 | 19.82 | 11.37 | 0.82 | 8.78 | 21.67 | 29.27 | 16.39 | 15.72 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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